Research Topics
| Pieter MestdaghSummaryAffiliation: Ghent University Country: Belgium Publications
| Collaborators
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Detail Information
Publications
The microRNA body map: dissecting microRNA function through integrative genomicsPieter Mestdagh
Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium and Life Technologies, Foster City, CA, USA
Nucleic Acids Res 39:e136. 2011..The miRNA bodymap project provides users with a single one-stop data-mining solution and has great potential to become a community resource...- A novel and universal method for microRNA RT-qPCR data normalizationPieter Mestdagh
Center for Medical Genetics, Ghent University Hospital, De Pintelaan 185, Ghent, Belgium
Genome Biol 10:R64. 2009..We demonstrate that the mean expression value outperforms the current normalization strategy in terms of better reduction of technical variation and more accurate appreciation of biological changes... - MicroRNA expression profiling to identify and validate reference genes for relative quantification in colorectal cancerKah Hoong Chang
Department of Surgery, National University of Ireland, Galway, Republic of Ireland
BMC Cancer 10:173. 2010..However, few reports detailed a robust identification and validation strategy for suitable reference genes for normalisation in miRNA RT-qPCR studies... - High-throughput stem-loop RT-qPCR miRNA expression profiling using minute amounts of input RNAPieter Mestdagh
Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium
Nucleic Acids Res 36:e143. 2008..The high specificity of RT-qPCR together with a superior sensitivity makes this approach the method of choice for high-throughput miRNA expression profiling... 
Comparison of miRNA profiles of microdissected Hodgkin/Reed-Sternberg cells and Hodgkin cell lines versus CD77+ B-cells reveals a distinct subset of differentially expressed miRNAsPieter Van Vlierberghe
Centre for Medical Genetics CMGG, Ghent University Hospital, Ghent, Belgium
Br J Haematol 147:686-90. 2009..Our data suggest that miRNAs are implicated in the pathogenesis of Hodgkin lymphoma and prompt further investigations concerning their role in cHL...- miRNA expression profiling enables risk stratification in archived and fresh neuroblastoma tumor samplesKatleen De Preter
Center for Medical Genetics Department of Paediatric Hematology and Oncology, Ghent University Hospital, Ghent, Belgium
Clin Cancer Res 17:7684-92. 2011..In this study, we aimed to establish and validate a prognostic miRNA signature for children with NB and tested it in both fresh frozen and archived formalin-fixed paraffin-embedded (FFPE) samples... - Dickkopf-3 is regulated by the MYCN-induced miR-17-92 cluster in neuroblastomaSara De Brouwer
Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium
Int J Cancer 130:2591-8. 2012..The strict MYCN-mediated regulation of DKK3 is suggestive for an important downstream function of the MYCN protein and thus warrants further investigations to unravel the role of DKK3 in NB... - External oligonucleotide standards enable cross laboratory comparison and exchange of real-time quantitative PCR dataJoelle Vermeulen
Center for Medical Genetics, Ghent University Hospital, Belgium
Nucleic Acids Res 37:e138. 2009.... - Whole-genome RT-qPCR microRNA expression profilingPieter Mestdagh
Center for Medical Genetics, Ghent University, De Pintelaan 185, Ghent 9000, Belgium
Methods Mol Biol 815:121-30. 2012..Owing to their small size (20-22 nucleotides), accurate quantification of miRNA expression is particularly challenging. In this chapter, we present different RT-qPCR technologies that enable whole genome miRNA expression quantification... - EVI1-mediated down regulation of MIR449A is essential for the survival of EVI1 positive leukaemic cellsAn De Weer
Centre for Medical Genetics, Ghent University Hospital, Ghent, Belgium
Br J Haematol 154:337-48. 2011..This EVI1-MIR449A-NOTCH1/BCL2 regulatory axis might open new possibilities for the development of therapeutic strategies in this poor prognostic leukaemia subgroup... - Focal DNA copy number changes in neuroblastoma target MYCN regulated genesCandy Kumps
Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium
PLoS ONE 8:e52321. 2013.... 
Predicting outcomes for children with neuroblastomaJoelle Vermeulen
Center for Medical Genetics, Ghent University Hospital, Ghent, B 9000, Belgium
Discov Med 10:29-36. 2010..Various studies have put forward new prognostic markers, including copy number aberrations, gene expression signatures, and epigenetic markers...- MicroRNA expression in induced sputum of smokers and patients with chronic obstructive pulmonary diseaseGeert R Van Pottelberge
Department of Respiratory Medicine, Ghent University Hospital, Ghent, Belgium
Am J Respir Crit Care Med 183:898-906. 2011..MicroRNAs (miRNAs) have the ability to regulate these processes by interfering with gene transcription and translation... - The emerging molecular pathogenesis of neuroblastoma: implications for improved risk assessment and targeted therapyNadine Van Roy
Center for Medical Genetics, Ghent University Hospital, De Pintelaan 185, B 9000 Ghent, Belgium
Genome Med 1:74. 2009..The recent successes in unraveling the genetic basis of neuroblastoma are now opening opportunities for development of targeted therapies... - The miR-17-92 microRNA cluster regulates multiple components of the TGF-β pathway in neuroblastomaPieter Mestdagh
Center for Medical Genetics, Ghent University Hospital, B 9000 Ghent, Belgium
Mol Cell 40:762-73. 2010..By functioning both upstream and downstream of pSMAD2, miR-17-92 activation triggers downregulation of multiple key effectors along the TGF-β signaling cascade as well as direct inhibition of TGF-β-responsive genes... - Identification of miR-145 as a key regulator of the pigmentary processPeter Dynoodt
Department of Dermatology, Ghent University Hospital, Ghent, Belgium
J Invest Dermatol 133:201-9. 2013..The significant down- or upregulation of major pigmentation genes, after modulating miR-145 expression, suggests a key role for miR-145 in regulating melanogenesis... - LNCipedia: a database for annotated human lncRNA transcript sequences and structuresPieter Jan Volders
Center for Medical Genetics, Ghent University, 9000 Ghent, Belgium
Nucleic Acids Res 41:D246-51. 2013..The database may serve as a resource to initiate small- and large-scale lncRNA studies. As an example, the LNCipedia content was used to develop a custom microarray for expression profiling of all available lncRNAs...