Pieter Mestdagh

Summary

Affiliation: Ghent University
Country: Belgium

Publications

  1. pmc The microRNA body map: dissecting microRNA function through integrative genomics
    Pieter Mestdagh
    Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium and Life Technologies, Foster City, CA, USA
    Nucleic Acids Res 39:e136. 2011
  2. pmc A novel and universal method for microRNA RT-qPCR data normalization
    Pieter Mestdagh
    Center for Medical Genetics, Ghent University Hospital, De Pintelaan 185, Ghent, Belgium
    Genome Biol 10:R64. 2009
  3. pmc MicroRNA expression profiling to identify and validate reference genes for relative quantification in colorectal cancer
    Kah Hoong Chang
    Department of Surgery, National University of Ireland, Galway, Republic of Ireland
    BMC Cancer 10:173. 2010
  4. pmc Focal DNA copy number changes in neuroblastoma target MYCN regulated genes
    Candy Kumps
    Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium
    PLoS ONE 8:e52321. 2013
  5. pmc Effective Alu repeat based RT-Qpcr normalization in cancer cell perturbation experiments
    Ali Rihani
    Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium
    PLoS ONE 8:e71776. 2013
  6. pmc High-throughput stem-loop RT-qPCR miRNA expression profiling using minute amounts of input RNA
    Pieter Mestdagh
    Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium
    Nucleic Acids Res 36:e143. 2008
  7. doi request reprint Comparison of miRNA profiles of microdissected Hodgkin/Reed-Sternberg cells and Hodgkin cell lines versus CD77+ B-cells reveals a distinct subset of differentially expressed miRNAs
    Pieter Van Vlierberghe
    Centre for Medical Genetics CMGG, Ghent University Hospital, Ghent, Belgium
    Br J Haematol 147:686-90. 2009
  8. pmc miRNA expression profiling enables risk stratification in archived and fresh neuroblastoma tumor samples
    Katleen De Preter
    Center for Medical Genetics Department of Paediatric Hematology and Oncology, Ghent University Hospital, Ghent, Belgium
    Clin Cancer Res 17:7684-92. 2011
  9. doi request reprint Dickkopf-3 is regulated by the MYCN-induced miR-17-92 cluster in neuroblastoma
    Sara De Brouwer
    Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium
    Int J Cancer 130:2591-8. 2012
  10. pmc External oligonucleotide standards enable cross laboratory comparison and exchange of real-time quantitative PCR data
    Joelle Vermeulen
    Center for Medical Genetics, Ghent University Hospital, Belgium
    Nucleic Acids Res 37:e138. 2009

Collaborators

Detail Information

Publications33

  1. pmc The microRNA body map: dissecting microRNA function through integrative genomics
    Pieter Mestdagh
    Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium and Life Technologies, Foster City, CA, USA
    Nucleic Acids Res 39:e136. 2011
    ..The miRNA bodymap project provides users with a single one-stop data-mining solution and has great potential to become a community resource...
  2. pmc A novel and universal method for microRNA RT-qPCR data normalization
    Pieter Mestdagh
    Center for Medical Genetics, Ghent University Hospital, De Pintelaan 185, Ghent, Belgium
    Genome Biol 10:R64. 2009
    ..We demonstrate that the mean expression value outperforms the current normalization strategy in terms of better reduction of technical variation and more accurate appreciation of biological changes...
  3. pmc MicroRNA expression profiling to identify and validate reference genes for relative quantification in colorectal cancer
    Kah Hoong Chang
    Department of Surgery, National University of Ireland, Galway, Republic of Ireland
    BMC Cancer 10:173. 2010
    ..However, few reports detailed a robust identification and validation strategy for suitable reference genes for normalisation in miRNA RT-qPCR studies...
  4. pmc Focal DNA copy number changes in neuroblastoma target MYCN regulated genes
    Candy Kumps
    Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium
    PLoS ONE 8:e52321. 2013
    ....
  5. pmc Effective Alu repeat based RT-Qpcr normalization in cancer cell perturbation experiments
    Ali Rihani
    Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium
    PLoS ONE 8:e71776. 2013
    ....
  6. pmc High-throughput stem-loop RT-qPCR miRNA expression profiling using minute amounts of input RNA
    Pieter Mestdagh
    Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium
    Nucleic Acids Res 36:e143. 2008
    ..The high specificity of RT-qPCR together with a superior sensitivity makes this approach the method of choice for high-throughput miRNA expression profiling...
  7. doi request reprint Comparison of miRNA profiles of microdissected Hodgkin/Reed-Sternberg cells and Hodgkin cell lines versus CD77+ B-cells reveals a distinct subset of differentially expressed miRNAs
    Pieter Van Vlierberghe
    Centre for Medical Genetics CMGG, Ghent University Hospital, Ghent, Belgium
    Br J Haematol 147:686-90. 2009
    ..Our data suggest that miRNAs are implicated in the pathogenesis of Hodgkin lymphoma and prompt further investigations concerning their role in cHL...
  8. pmc miRNA expression profiling enables risk stratification in archived and fresh neuroblastoma tumor samples
    Katleen De Preter
    Center for Medical Genetics Department of Paediatric Hematology and Oncology, Ghent University Hospital, Ghent, Belgium
    Clin Cancer Res 17:7684-92. 2011
    ..In this study, we aimed to establish and validate a prognostic miRNA signature for children with NB and tested it in both fresh frozen and archived formalin-fixed paraffin-embedded (FFPE) samples...
  9. doi request reprint Dickkopf-3 is regulated by the MYCN-induced miR-17-92 cluster in neuroblastoma
    Sara De Brouwer
    Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium
    Int J Cancer 130:2591-8. 2012
    ..The strict MYCN-mediated regulation of DKK3 is suggestive for an important downstream function of the MYCN protein and thus warrants further investigations to unravel the role of DKK3 in NB...
  10. pmc External oligonucleotide standards enable cross laboratory comparison and exchange of real-time quantitative PCR data
    Joelle Vermeulen
    Center for Medical Genetics, Ghent University Hospital, Belgium
    Nucleic Acids Res 37:e138. 2009
    ....
  11. doi request reprint EVI1-mediated down regulation of MIR449A is essential for the survival of EVI1 positive leukaemic cells
    An De Weer
    Centre for Medical Genetics, Ghent University Hospital, Ghent, Belgium
    Br J Haematol 154:337-48. 2011
    ..This EVI1-MIR449A-NOTCH1/BCL2 regulatory axis might open new possibilities for the development of therapeutic strategies in this poor prognostic leukaemia subgroup...
  12. doi request reprint Whole-genome RT-qPCR microRNA expression profiling
    Pieter Mestdagh
    Center for Medical Genetics, Ghent University, De Pintelaan 185, Ghent 9000, Belgium
    Methods Mol Biol 815:121-30. 2012
    ..Owing to their small size (20-22 nucleotides), accurate quantification of miRNA expression is particularly challenging. In this chapter, we present different RT-qPCR technologies that enable whole genome miRNA expression quantification...
  13. pmc Genome wide expression profiling of p53 regulated miRNAs in neuroblastoma
    Ali Rihani
    Center for Medical Genetics, Ghent University, De Pintelaan 185, B 9000 Ghent, Belgium
    Sci Rep 5:9027. 2015
    ..Taken together, our findings yield new insights in the network of p53-regulated miRNAs in neuroblastoma. ..
  14. pmc MicroRNA-128-3p is a novel oncomiR targeting PHF6 in T-cell acute lymphoblastic leukemia
    Evelien Mets
    Center for Medical Genetics, Ghent University, Belgium
    Haematologica 99:1326-33. 2014
    ..We conclude that miR-128-3p is a strong novel candidate oncogenic microRNA in T-cell acute lymphoblastic leukemia which targets the PHF6 tumor suppressor gene. ..
  15. pmc MYCN-targeting miRNAs are predominantly downregulated during MYCN‑driven neuroblastoma tumor formation
    Anneleen Beckers
    Center for Medical Genetics CMGG, Ghent University, Ghent, Belgium
    Oncotarget 6:5204-16. 2015
    ..Therefore, we provide evidence that MYCN-targeting miRNAs are preferentially downregulated in MYCN-driven neuroblastoma, suggesting that MYCN negatively controls the expression of these miRNAs, to safeguard its expression. ..
  16. pmc Regulatory microRNA network identification in bovine blastocyst development
    Karen Goossens
    Department of Nutrition, Genetics and Ethology, Ghent University, Merelbeke, Belgium
    Stem Cells Dev 22:1907-20. 2013
    ..The results of this study expand our knowledge about the miRNA signature of the bovine blastocyst and of the interactions between miRNAs and cell fate regulating transcription factors...
  17. ncbi request reprint Predicting outcomes for children with neuroblastoma
    Joelle Vermeulen
    Center for Medical Genetics, Ghent University Hospital, Ghent, B 9000, Belgium
    Discov Med 10:29-36. 2010
    ..Various studies have put forward new prognostic markers, including copy number aberrations, gene expression signatures, and epigenetic markers...
  18. pmc CLL cells respond to B-Cell receptor stimulation with a microRNA/mRNA signature associated with MYC activation and cell cycle progression
    Valerie Pede
    Department of Clinical Chemistry, Microbiology and Immunology Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
    PLoS ONE 8:e60275. 2013
    ....
  19. doi request reprint Identification of miR-145 as a key regulator of the pigmentary process
    Peter Dynoodt
    Department of Dermatology, Ghent University Hospital, Ghent, Belgium
    J Invest Dermatol 133:201-9. 2013
    ..The significant down- or upregulation of major pigmentation genes, after modulating miR-145 expression, suggests a key role for miR-145 in regulating melanogenesis...
  20. doi request reprint MicroRNA expression in induced sputum of smokers and patients with chronic obstructive pulmonary disease
    Geert R Van Pottelberge
    Department of Respiratory Medicine, Ghent University Hospital, Ghent, Belgium
    Am J Respir Crit Care Med 183:898-906. 2011
    ..MicroRNAs (miRNAs) have the ability to regulate these processes by interfering with gene transcription and translation...
  21. pmc An update on LNCipedia: a database for annotated human lncRNA sequences
    Pieter Jan Volders
    Center for Medical Genetics, Ghent University, Ghent 9000, Belgium
    Nucleic Acids Res 43:D174-80. 2015
    ..In addition, a tool to assess lncRNA gene conservation between human, mouse and zebrafish has been implemented...
  22. pmc The impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling
    Jan Van Deun
    Laboratory of Experimental Cancer Research, Department of Radiation Oncology and Experimental Cancer Research, Ghent University Hospital, Ghent, Belgium
    J Extracell Vesicles 3:. 2014
    ..Our results demonstrate that implementation of high purification techniques is a prerequisite to obtain reliable omics data and identify exosome-specific functions and biomarkers...
  23. doi request reprint Non-coding RNAs in the pathogenesis of COPD
    Elise G De Smet
    Laboratory for Translational Research in Obstructive Pulmonary Diseases, Department of Respiratory Medicine, Ghent University Hospital, Ghent, Belgium
    Thorax 70:782-91. 2015
    ..In addition, we briefly discuss a specific lncRNA that is upregulated upon cigarette smoke exposure, providing a short introduction to this more recently discovered group of non-coding RNAs. ..
  24. pmc The emerging molecular pathogenesis of neuroblastoma: implications for improved risk assessment and targeted therapy
    Nadine Van Roy
    Center for Medical Genetics, Ghent University Hospital, De Pintelaan 185, B 9000 Ghent, Belgium
    Genome Med 1:74. 2009
    ..The recent successes in unraveling the genetic basis of neuroblastoma are now opening opportunities for development of targeted therapies...
  25. pmc The miR-17-92 microRNA cluster regulates multiple components of the TGF-β pathway in neuroblastoma
    Pieter Mestdagh
    Center for Medical Genetics, Ghent University Hospital, B 9000 Ghent, Belgium
    Mol Cell 40:762-73. 2010
    ..By functioning both upstream and downstream of pSMAD2, miR-17-92 activation triggers downregulation of multiple key effectors along the TGF-β signaling cascade as well as direct inhibition of TGF-β-responsive genes...
  26. pmc LNCipedia: a database for annotated human lncRNA transcript sequences and structures
    Pieter Jan Volders
    Center for Medical Genetics, Ghent University, 9000 Ghent, Belgium
    Nucleic Acids Res 41:D246-51. 2013
    ..The database may serve as a resource to initiate small- and large-scale lncRNA studies. As an example, the LNCipedia content was used to develop a custom microarray for expression profiling of all available lncRNAs...
  27. doi request reprint RT-qPCR-Based Quantification of Small Non-Coding RNAs
    Fjoralba Zeka
    Center for Medical Genetics, Ghent University, Ghent, Belgium
    Methods Mol Biol 1296:85-102. 2015
    ..We conclude by demonstrating raw data preprocessing and normalization using expression data obtained from high-throughput miRNA profiling of human RNA samples. ..
  28. pmc The Notch driven long non-coding RNA repertoire in T-cell acute lymphoblastic leukemia
    Kaat Durinck
    Center for Medical Genetics, Ghent University, Belgium
    Haematologica 99:1808-16. 2014
    ....
  29. doi request reprint Cancer-associated adipose tissue promotes breast cancer progression by paracrine oncostatin M and Jak/STAT3 signaling
    Lore Lapeire
    Department of Medical Oncology, Ghent University Hospital, Ghent, Belgium
    Cancer Res 74:6806-19. 2014
    ..Our work reveals the potential of novel therapeutic strategies targeting the OSM and STAT3 axis in patients with breast cancer harboring nuclear pSTAT3-Y705...
  30. doi request reprint Evaluation of quantitative miRNA expression platforms in the microRNA quality control (miRQC) study
    Pieter Mestdagh
    Center for Medical Genetics, Ghent University, Ghent, Belgium
    Nat Methods 11:809-15. 2014
    ..The results indicate that each method has its strengths and weaknesses, which help to guide informed selection of a quantitative microRNA gene expression platform for particular study goals. ..
  31. doi request reprint Non-coding RNAs and respiratory disease
    Pieter Mestdagh
    Center for Medical Genetics, Ghent University, Ghent, Belgium
    Thorax 70:388-90. 2015
    ..In this concise review, we will provide essential concepts of ncRNA science, with special emphasis on discoveries relevant to the pulmonary physician. ..
  32. doi request reprint Lung tumours reprogram pulmonary dendritic cell immunogenicity at the microRNA level
    Lotte Pyfferoen
    Department of Respiratory Medicine, Tumor Immunology Laboratory, Ghent University Hospital, Ghent, Belgium VIB Inflammation Research Center, Ghent, Belgium Department of Respiratory Medicine, Ghent University, Ghent, Belgium
    Int J Cancer 135:2868-77. 2014
    ..Taken together, our data reveal that lung tumours induce an immunosuppressive microRNA signature in pulmonary DCs. Interfering with the DC-intrinsic capacity to remodel microRNA repertoires affects lung tumour outcome...
  33. doi request reprint IL-1β potently stabilizes IL-6 mRNA in human astrocytes
    Anneleen Spooren
    Laboratory of Eukaryotic Gene Expression and Signal Transduction LEGEST, University of Ghent, K L Ledeganckstraat 35, 9000 Ghent, Belgium
    Biochem Pharmacol 81:1004-15. 2011
    ..Our data using pharmacological inhibitors suggest PKC is an important modulator of IL-6 expression in the central nervous system and this might have therapeutic implications...