Pieter Mestdagh

Summary

Affiliation: Ghent University
Country: Belgium

Publications

  1. pmc LNCipedia: a database for annotated human lncRNA transcript sequences and structures
    Pieter Jan Volders
    Center for Medical Genetics, Ghent University, 9000 Ghent, Belgium
    Nucleic Acids Res 41:D246-51. 2013
  2. pmc The microRNA body map: dissecting microRNA function through integrative genomics
    Pieter Mestdagh
    Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium and Life Technologies, Foster City, CA, USA
    Nucleic Acids Res 39:e136. 2011
  3. pmc A novel and universal method for microRNA RT-qPCR data normalization
    Pieter Mestdagh
    Center for Medical Genetics, Ghent University Hospital, De Pintelaan 185, Ghent, Belgium
    Genome Biol 10:R64. 2009
  4. pmc MicroRNA expression profiling to identify and validate reference genes for relative quantification in colorectal cancer
    Kah Hoong Chang
    Department of Surgery, National University of Ireland, Galway, Republic of Ireland
    BMC Cancer 10:173. 2010
  5. pmc Focal DNA copy number changes in neuroblastoma target MYCN regulated genes
    Candy Kumps
    Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium
    PLoS ONE 8:e52321. 2013
  6. pmc Effective Alu repeat based RT-Qpcr normalization in cancer cell perturbation experiments
    Ali Rihani
    Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium
    PLoS ONE 8:e71776. 2013
  7. pmc High-throughput stem-loop RT-qPCR miRNA expression profiling using minute amounts of input RNA
    Pieter Mestdagh
    Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium
    Nucleic Acids Res 36:e143. 2008
  8. doi request reprint Comparison of miRNA profiles of microdissected Hodgkin/Reed-Sternberg cells and Hodgkin cell lines versus CD77+ B-cells reveals a distinct subset of differentially expressed miRNAs
    Pieter Van Vlierberghe
    Centre for Medical Genetics CMGG, Ghent University Hospital, Ghent, Belgium
    Br J Haematol 147:686-90. 2009
  9. doi request reprint EVI1-mediated down regulation of MIR449A is essential for the survival of EVI1 positive leukaemic cells
    An De Weer
    Centre for Medical Genetics, Ghent University Hospital, Ghent, Belgium
    Br J Haematol 154:337-48. 2011
  10. doi request reprint miRNA expression profiling enables risk stratification in archived and fresh neuroblastoma tumor samples
    Katleen De Preter
    Center for Medical Genetics Department of Paediatric Hematology and Oncology, Ghent University Hospital, Ghent, Belgium
    Clin Cancer Res 17:7684-92. 2011

Collaborators

Detail Information

Publications21

  1. pmc LNCipedia: a database for annotated human lncRNA transcript sequences and structures
    Pieter Jan Volders
    Center for Medical Genetics, Ghent University, 9000 Ghent, Belgium
    Nucleic Acids Res 41:D246-51. 2013
    ..The database may serve as a resource to initiate small- and large-scale lncRNA studies. As an example, the LNCipedia content was used to develop a custom microarray for expression profiling of all available lncRNAs...
  2. pmc The microRNA body map: dissecting microRNA function through integrative genomics
    Pieter Mestdagh
    Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium and Life Technologies, Foster City, CA, USA
    Nucleic Acids Res 39:e136. 2011
    ..The miRNA bodymap project provides users with a single one-stop data-mining solution and has great potential to become a community resource...
  3. pmc A novel and universal method for microRNA RT-qPCR data normalization
    Pieter Mestdagh
    Center for Medical Genetics, Ghent University Hospital, De Pintelaan 185, Ghent, Belgium
    Genome Biol 10:R64. 2009
    ..We demonstrate that the mean expression value outperforms the current normalization strategy in terms of better reduction of technical variation and more accurate appreciation of biological changes...
  4. pmc MicroRNA expression profiling to identify and validate reference genes for relative quantification in colorectal cancer
    Kah Hoong Chang
    Department of Surgery, National University of Ireland, Galway, Republic of Ireland
    BMC Cancer 10:173. 2010
    ..However, few reports detailed a robust identification and validation strategy for suitable reference genes for normalisation in miRNA RT-qPCR studies...
  5. pmc Focal DNA copy number changes in neuroblastoma target MYCN regulated genes
    Candy Kumps
    Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium
    PLoS ONE 8:e52321. 2013
    ....
  6. pmc Effective Alu repeat based RT-Qpcr normalization in cancer cell perturbation experiments
    Ali Rihani
    Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium
    PLoS ONE 8:e71776. 2013
    ....
  7. pmc High-throughput stem-loop RT-qPCR miRNA expression profiling using minute amounts of input RNA
    Pieter Mestdagh
    Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium
    Nucleic Acids Res 36:e143. 2008
    ..The high specificity of RT-qPCR together with a superior sensitivity makes this approach the method of choice for high-throughput miRNA expression profiling...
  8. doi request reprint Comparison of miRNA profiles of microdissected Hodgkin/Reed-Sternberg cells and Hodgkin cell lines versus CD77+ B-cells reveals a distinct subset of differentially expressed miRNAs
    Pieter Van Vlierberghe
    Centre for Medical Genetics CMGG, Ghent University Hospital, Ghent, Belgium
    Br J Haematol 147:686-90. 2009
    ..Our data suggest that miRNAs are implicated in the pathogenesis of Hodgkin lymphoma and prompt further investigations concerning their role in cHL...
  9. doi request reprint EVI1-mediated down regulation of MIR449A is essential for the survival of EVI1 positive leukaemic cells
    An De Weer
    Centre for Medical Genetics, Ghent University Hospital, Ghent, Belgium
    Br J Haematol 154:337-48. 2011
    ..This EVI1-MIR449A-NOTCH1/BCL2 regulatory axis might open new possibilities for the development of therapeutic strategies in this poor prognostic leukaemia subgroup...
  10. doi request reprint miRNA expression profiling enables risk stratification in archived and fresh neuroblastoma tumor samples
    Katleen De Preter
    Center for Medical Genetics Department of Paediatric Hematology and Oncology, Ghent University Hospital, Ghent, Belgium
    Clin Cancer Res 17:7684-92. 2011
    ..In this study, we aimed to establish and validate a prognostic miRNA signature for children with NB and tested it in both fresh frozen and archived formalin-fixed paraffin-embedded (FFPE) samples...
  11. doi request reprint Dickkopf-3 is regulated by the MYCN-induced miR-17-92 cluster in neuroblastoma
    Sara De Brouwer
    Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium
    Int J Cancer 130:2591-8. 2012
    ..The strict MYCN-mediated regulation of DKK3 is suggestive for an important downstream function of the MYCN protein and thus warrants further investigations to unravel the role of DKK3 in NB...
  12. pmc External oligonucleotide standards enable cross laboratory comparison and exchange of real-time quantitative PCR data
    Joelle Vermeulen
    Center for Medical Genetics, Ghent University Hospital, Belgium
    Nucleic Acids Res 37:e138. 2009
    ....
  13. doi request reprint Whole-genome RT-qPCR microRNA expression profiling
    Pieter Mestdagh
    Center for Medical Genetics, Ghent University, De Pintelaan 185, Ghent 9000, Belgium
    Methods Mol Biol 815:121-30. 2012
    ..Owing to their small size (20-22 nucleotides), accurate quantification of miRNA expression is particularly challenging. In this chapter, we present different RT-qPCR technologies that enable whole genome miRNA expression quantification...
  14. pmc Regulatory microRNA network identification in bovine blastocyst development
    Karen Goossens
    Department of Nutrition, Genetics and Ethology, Ghent University, Merelbeke, Belgium
    Stem Cells Dev 22:1907-20. 2013
    ..The results of this study expand our knowledge about the miRNA signature of the bovine blastocyst and of the interactions between miRNAs and cell fate regulating transcription factors...
  15. ncbi request reprint Predicting outcomes for children with neuroblastoma
    Joelle Vermeulen
    Center for Medical Genetics, Ghent University Hospital, Ghent, B 9000, Belgium
    Discov Med 10:29-36. 2010
    ..Various studies have put forward new prognostic markers, including copy number aberrations, gene expression signatures, and epigenetic markers...
  16. pmc CLL cells respond to B-Cell receptor stimulation with a microRNA/mRNA signature associated with MYC activation and cell cycle progression
    Valerie Pede
    Department of Clinical Chemistry, Microbiology and Immunology Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
    PLoS ONE 8:e60275. 2013
    ....
  17. doi request reprint Identification of miR-145 as a key regulator of the pigmentary process
    Peter Dynoodt
    Department of Dermatology, Ghent University Hospital, Ghent, Belgium
    J Invest Dermatol 133:201-9. 2013
    ..The significant down- or upregulation of major pigmentation genes, after modulating miR-145 expression, suggests a key role for miR-145 in regulating melanogenesis...
  18. doi request reprint MicroRNA expression in induced sputum of smokers and patients with chronic obstructive pulmonary disease
    Geert R Van Pottelberge
    Department of Respiratory Medicine, Ghent University Hospital, Ghent, Belgium
    Am J Respir Crit Care Med 183:898-906. 2011
    ..MicroRNAs (miRNAs) have the ability to regulate these processes by interfering with gene transcription and translation...
  19. pmc The miR-17-92 microRNA cluster regulates multiple components of the TGF-β pathway in neuroblastoma
    Pieter Mestdagh
    Center for Medical Genetics, Ghent University Hospital, B 9000 Ghent, Belgium
    Mol Cell 40:762-73. 2010
    ..By functioning both upstream and downstream of pSMAD2, miR-17-92 activation triggers downregulation of multiple key effectors along the TGF-β signaling cascade as well as direct inhibition of TGF-β-responsive genes...
  20. pmc The emerging molecular pathogenesis of neuroblastoma: implications for improved risk assessment and targeted therapy
    Nadine Van Roy
    Center for Medical Genetics, Ghent University Hospital, De Pintelaan 185, B 9000 Ghent, Belgium
    Genome Med 1:74. 2009
    ..The recent successes in unraveling the genetic basis of neuroblastoma are now opening opportunities for development of targeted therapies...
  21. doi request reprint IL-1β potently stabilizes IL-6 mRNA in human astrocytes
    Anneleen Spooren
    Laboratory of Eukaryotic Gene Expression and Signal Transduction LEGEST, University of Ghent, K L Ledeganckstraat 35, 9000 Ghent, Belgium
    Biochem Pharmacol 81:1004-15. 2011
    ..Our data using pharmacological inhibitors suggest PKC is an important modulator of IL-6 expression in the central nervous system and this might have therapeutic implications...