Björn Heindryckx


Affiliation: Ghent University
Country: Belgium


  1. Taelman J, Popovic M, Bialecka M, Tilleman L, Warrier S, Van der Jeught M, et al. WNT inhibition and increased FGF signalling promotes derivation of less heterogeneous primed human embryonic stem cells, compatible with differentiation. Stem Cells Dev. 2019;: pubmed publisher
    ..Overall, DhiFI hESCs retained advantages of both primed and naive pluripotency and may ultimately represent a more favourable starting point for differentiation towards clinically desired cell types. ..
  2. Ferrer Buitrago M, Bonte D, Dhaenens L, Vermorgen S, Lu Y, De Sutter P, et al. Assessment of the calcium releasing machinery in oocytes that failed to fertilize after conventional ICSI and assisted oocyte activation. Reprod Biomed Online. 2019;38:497-507 pubmed publisher
  3. Heindryckx B, Neupane J, Vandewoestyne M, Christodoulou C, Jackers Y, Gerris J, et al. Mutation-free baby born from a mitochondrial encephalopathy, lactic acidosis and stroke-like syndrome carrier after blastocyst trophectoderm preimplantation genetic diagnosis. Mitochondrion. 2014;18:12-7 pubmed publisher
    ..95). This is the first case of mutation-free baby born from a MELAS patient after TE biopsy and supports the applicability of blastocyst PGD for patients with mtDNA disorders to establish healthy offspring. ..
  4. Neupane J, Vandewoestyne M, Ghimire S, Lu Y, Qian C, Van Coster R, et al. Assessment of nuclear transfer techniques to prevent the transmission of heritable mitochondrial disorders without compromising embryonic development competence in mice. Mitochondrion. 2014;18:27-33 pubmed publisher
    ..We show that NT in the germ line is potent to prevent transmission of heritable mtDNA disorders with the applicability for patients attempting reproduction. ..
  5. Ghimire S, Van der Jeught M, Neupane J, Roost M, Anckaert J, Popovic M, et al. Comparative analysis of naive, primed and ground state pluripotency in mouse embryonic stem cells originating from the same genetic background. Sci Rep. 2018;8:5884 pubmed publisher
    ..We further demonstrate that the differentiation propensity of mESCs to specific germ layers is highly dependent on their respective state of pluripotency. ..
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    Heindryckx B, Van der Elst J, De Sutter P, Dhont M. Treatment option for sperm- or oocyte-related fertilization failure: assisted oocyte activation following diagnostic heterologous ICSI. Hum Reprod. 2005;20:2237-41 pubmed
    ..Assisted oocyte activation enables normal fertilization and pregnancy in sperm- and oocyte-related fertilization failure. ..
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    Heindryckx B, De Sutter P, Gerris J, Dhont M, Van der Elst J. Embryo development after successful somatic cell nuclear transfer to in vitro matured human germinal vesicle oocytes. Hum Reprod. 2007;22:1982-90 pubmed
    ..To our knowledge, this is the first report describing development of cloned human embryos using IVM oocytes and non-autologous transfer using a conventional method of SCNT. ..
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    Heindryckx B, De Gheselle S, Gerris J, Dhont M, De Sutter P. Efficiency of assisted oocyte activation as a solution for failed intracytoplasmic sperm injection. Reprod Biomed Online. 2008;17:662-8 pubmed
    ..When MOAT reveals no activation deficiency in spermatozoa, AOA still allows for high fertilization and acceptable pregnancy rates. The obstetric and neonatal outcomes after AOA were normal as no malformations were observed. ..
  9. Duggal G, Heindryckx B, Warrier S, Taelman J, Van der Jeught M, Deforce D, et al. Exogenous supplementation of Activin A enhances germ cell differentiation of human embryonic stem cells. Mol Hum Reprod. 2015;21:410-23 pubmed publisher
    ..We propose a novel relationship between the endoderm and germ cell lineage during hESC differentiation. ..

More Information


  1. Van der Jeught M, O Leary T, Duggal G, De Sutter P, Chuva de Sousa Lopes S, Heindryckx B. The post-inner cell mass intermediate: implications for stem cell biology and assisted reproductive technology. Hum Reprod Update. 2015;21:616-26 pubmed publisher
    ..We will now further investigate how these intermediate and/or different pluripotent stages may impact the use of human stem cells in regenerative medicine and assisted reproductive technology. ..
  2. Neupane J, Ghimire S, Vandewoestyne M, Lu Y, Gerris J, Van Coster R, et al. Cellular Heterogeneity in the Level of mtDNA Heteroplasmy in Mouse Embryonic Stem Cells. Cell Rep. 2015;13:1304-1309 pubmed publisher
    ..Furthermore, proliferation of one mtDNA genotype over another may pose the risk of accumulating mutant mtDNAs during subsequent cell divisions. ..
  3. Nikiforaki D, Vanden Meerschaut F, De Roo C, Lu Y, Ferrer Buitrago M, De Sutter P, et al. Effect of two assisted oocyte activation protocols used to overcome fertilization failure on the activation potential and calcium releasing pattern. Fertil Steril. 2016;105:798-806.e2 pubmed publisher
    ..Bearing in mind that mammalian fertilization is successful when the total dose of Ca(2+) released reaches a minimal threshold, the use of ionomycin for human AOA might be justified instead of the use of A23187. ..
  4. Warrier S, Popovic M, Van der Jeught M, Heindryckx B. Establishment and Characterization of Naïve Pluripotency in Human Embryonic Stem Cells. Methods Mol Biol. 2016;1516:13-46 pubmed publisher
    ..Conversion of primed human embryonic stem cells towards the naive pluripotent state should be confirmed by a detailed characterization of the cells, as described in this chapter. ..
  5. Ferrer Buitrago M, Bonte D, De Sutter P, Leybaert L, Heindryckx B. Single Ca2+ transients vs oscillatory Ca2+ signaling for assisted oocyte activation: limitations and benefits. Reproduction. 2018;155:R105-R119 pubmed publisher
    ..Furthermore, mechanisms of AOA and the limitations and benefits associated with the application of different activation agents are discussed. ..