Research Topics
Genomes and Genes
| Kris GevaertSummaryAffiliation: Ghent University Country: Belgium Publications
| Collaborators
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Detail Information
Publications
The expression, processing and localization of polymorphic membrane proteins in Chlamydia pneumoniae strain CWL029Brian Berg Vandahl
Department of Medical Microbiology and Immunology, University of Aarhus, Denmark
BMC Microbiol 2:36. 2002..In the present study, the expression, processing and localisation of the sixteen full-length Pmps in C. pneumoniae strain CWL029 have been further investigated by two-dimensional gel electrophoresis and immunofluorescence microscopy...
Protein identification based on matrix assisted laser desorption/ionization-post source decay-mass spectrometryK Gevaert
Flanders Interuniversity, Department of Medical Protein Research, Ghent University, Belgium
Electrophoresis 22:1645-51. 2001..The combination of peptide mass fingerprinting and PSD and analysis described here generally leads to unambiguous protein identification in the amount of material range generally encountered in most proteome studies...- Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptidesKris Gevaert
Department of Medical Protein Research, Flanders Interuniversity Institute for Biotechnology, Ghent University, A Baertsoenkaai 3, B 9000 Ghent, Belgium
Nat Biotechnol 21:566-9. 2003..Omitting the acetylation step results in the isolation of non-lysine-containing N-terminal peptides from in vivo blocked proteins... - In vitro and in vivo protein-bound tyrosine nitration characterized by diagonal chromatographyBart Ghesquière
Department of Medical Protein Research, VIB, B 9000 Ghent, Belgium
Mol Cell Proteomics 8:2642-52. 2009.... - GalNAc/Gal-binding Rhizoctonia solani agglutinin has antiproliferative activity in Drosophila melanogaster S2 cells via MAPK and JAK/STAT signalingMohamad Hamshou
Laboratory of Agrozoology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium
PLoS ONE 7:e33680. 2012..Finally, RSA affinity chromatography of membrane proteins from S2 cells allowed the identification of several cell surface receptors involved in both signaling transduction pathways... 
Selecting protein N-terminal peptides by combined fractional diagonal chromatographyAn Staes
Department of Medical Protein Research, Vlaams Instituut voor Biotechnologie VIB, Ghent, Belgium
Nat Protoc 6:1130-41. 2011..Completion of the N-terminal COFRADIC procedure takes ~5 d...- MS-driven protease substrate degradomicsFrancis Impens
Department of Medical Protein Research, VIB, Ghent, Belgium
Proteomics 10:1284-96. 2010..The most promising techniques are discussed in this review and we further elaborate on the bioinformatics challenges that accompany mainly qualitative, MS-driven protease substrate degradome studies... - The substrate specificity profile of human granzyme APetra Van Damme
Department of Medical Protein Research, Flanders Interuniversity Institute for Biotechnology, Ghent, Belgium
Biol Chem 391:983-97. 2010..Further substrate characterization was used to delineate physical properties in the substrate specificity profiles, which further highlights important aspects in protease/substrate biology... - Diversity in protein glycosylation among insect speciesGianni Vandenborre
Laboratory of Agrozoology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium
PLoS ONE 6:e16682. 2011..Although the Class of the Insecta is the largest animal taxon on Earth, almost all information concerning glycosylation in insects is derived from studies with only one species, namely the fruit fly Drosophila melanogaster... - PRIDE: the proteomics identifications databaseLennart Martens
Department of Biochemistry, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
Proteomics 5:3537-45. 2005..The complete PRIDE database, source code, data, and support tools are freely available for web access or download and local installation... - Redox proteomics of protein-bound methionine oxidationBart Ghesquière
Department of Medical Protein Research, VIB, B 9000 Ghent, Belgium
Mol Cell Proteomics 10:M110.006866. 2011..This was supported by a structural modeling of the MsrA catalytic center. Finally, we applied our method onto a serum proteome from a mouse sepsis model and identified 35 in vivo methionine oxidation events in 27 different proteins... - Analysis of protein processing by N-terminal proteomics reveals novel species-specific substrate determinants of granzyme B orthologsPetra Van Damme
Department of Medical Protein Research, Flanders Institute for Biotechnology VIB, Ghent, Belgium
Mol Cell Proteomics 8:258-72. 2009.... - A new approach for mapping sialylated N-glycosites in serum proteomesBart Ghesquière
Department of Medical Protein Research, VIB, B 9000 Ghent, Belgium
J Proteome Res 6:4304-12. 2007.... - Probing the efficiency of proteolytic events by positional proteomicsKim Plasman
Department of Medical Protein Research, VIB, Ghent, Belgium
Mol Cell Proteomics 10:M110.003301. 2011..Several of our proteomics results were further confirmed by substrate immunoblotting and by using peptide substrates incubated with human granzyme B... - A new functional, chemical proteomics technology to identify purine nucleotide binding sites in complex proteomesXavier Hanoulle
Department of Medical Protein Research and Biochemistry, Flanders Interuniversity Institute for Biotechnology and Faculty of Medicine and Health Sciences, Ghent University, A. Baertsoenkaai 3, B-9000 Ghent, Belgium
J Proteome Res 5:3438-45. 2006..When applied on a cellular level, 185 different FSBA-labeled sites in a human Jurkat cell lysate were identified. Functional and structural aspects of the use of FSBA on a proteome-wide scale are discussed... - A la carte proteomics with an emphasis on gel-free techniquesKris Gevaert
Department of Medical Protein Research, VIB, Ghent, Belgium
Proteomics 7:2698-718. 2007..Furthermore, different approaches for (multiplexed) gel-free proteome analysis are discussed... - Do we want our data raw? Including binary mass spectrometry data in public proteomics data repositoriesLennart Martens
Department of Biochemistry, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
Proteomics 5:3501-5. 2005..Finally, some suggestions are made for both immediate and future storage of MS data in public repositories... - Combining quantitative proteomics data processing workflows for greater sensitivityNiklaas Colaert
Department of Medical Protein Research, Vlaams Instituut voor Biotechnologie VIB, Ghent, Belgium
Nat Methods 8:481-3. 2011..Our integrative workflow is freely available through our user-friendly, open-source Rover software (http://compomics-rover.googlecode.com/)... - NatF contributes to an evolutionary shift in protein N-terminal acetylation and is important for normal chromosome segregationPetra Van Damme
Department of Medical Protein Research, Ghent University, Ghent, Belgium
PLoS Genet 7:e1002169. 2011..With the characterization of NatF, the co-translational N-Ac machinery appears complete since all the major substrate groups in eukaryotes are accounted for... - Bioinformatics analysis of a Saccharomyces cerevisiae N-terminal proteome provides evidence of alternative translation initiation and post-translational N-terminal acetylationKenny Helsens
Department of Medical Protein Research, VIB, B 9000 Ghent, Belgium
J Proteome Res 10:3578-89. 2011..Finally, we also observed an unexpected high number of N(α)-acetylated peptides that could not be related to TIS and therefore suggest events of post-translational N(α)-acetylation... - Interaction of the tobacco lectin with histone proteinsDieter Schouppe
Laboratory of Biochemistry and Glycobiology, Department of Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, B 9000 Ghent, Belgium
Plant Physiol 155:1091-102. 2011..Since the lectin-histone interaction was shown to be carbohydrate dependent, it is proposed that Nictaba might fulfill a signaling role in response to stress by interacting with O-GlcNAcylated proteins in the plant cell nucleus... - Applications of diagonal chromatography for proteome-wide characterization of protein modifications and activity-based analysesKris Gevaert
Department of Medical Protein Research, VIB, Ghent, Belgium
FEBS J 274:6277-89. 2007..Additionally, we discuss an activity-based proteome study in which purine-binding proteins were profiled by diagonal chromatography... - Improved recovery of proteome-informative, protein N-terminal peptides by combined fractional diagonal chromatography (COFRADIC)An Staes
Department of Medical Protein Research, VIB, Ghent, Belgium
Proteomics 8:1362-70. 2008..We further show that now close to 95% of all COFRADIC-sorted peptides are alpha-amino-acetylated and, using the same amount of starting material, our novel procedure leads to an increased number of protein identifications... - Rover: a tool to visualize and validate quantitative proteomics data from different sourcesNiklaas Colaert
Department of Medical Protein Research, VIB, Ghent, Belgium
Proteomics 10:1226-9. 2010.... - The actin propulsive machinery: the proteome of Listeria monocytogenes tailsMarleen Van Troys
Department of Medical Protein Research, VIB, B 9000 Ghent, Belgium
Biochem Biophys Res Commun 375:194-9. 2008..This work forms a new basis to expand current models of cellular protrusion... - Proteome-derived peptide libraries allow detailed analysis of the substrate specificities of N(alpha)-acetyltransferases and point to hNaa10p as the post-translational actin N(alpha)-acetyltransferasePetra Van Damme
Department of Medical Protein Research, VIB, B 9000 Ghent, Belgium
Mol Cell Proteomics 10:M110.004580. 2011..Thus, complex formation of NatA might alter the substrate specificity profile as compared with its isolated catalytic subunits, and, furthermore, NatA or hNaa10p may function as a post-translational actin N(α)-acetyltransferase... - A stringent approach to improve the quality of nitrotyrosine peptide identificationsBart Ghesquière
Department of Medical Protein Research, VIB, Ghent, Belgium
Proteomics 11:1094-8. 2011..Central in our approach is the reduction of nitrotyrosine into aminotyrosine and the use of the Peptizer algorithm to inspect MS/MS quality-related assumptions... - Assessing a novel microfluidic interface for shotgun proteome analysesAn Staes
Department of Medical Protein Research, VIB, Ghent, Belgium
J Sep Sci 30:1468-76. 2007.... - Caspase-specific and nonspecific in vivo protein processing during Fas-induced apoptosisPetra Van Damme
Department of Medical Protein Research, Flanders Interuniversity Institute for Biotechnology, Belgium
Nat Methods 2:771-7. 2005..The spliceosome complex appeared a preferred target, as 14 of its members were processed. Differential isotopic labeling further revealed specific release of nucleosomal components from apoptotic nuclei... - The human platelet proteome mapped by peptide-centric proteomics: a functional protein profileLennart Martens
Department of Medical Protein Research, Flanders Interuniversity Institute for Biotechnology, Department of Biochemistry, Ghent University, A. Baertsoenkaai 3, B-9000 Ghent, Belgium
Proteomics 5:3193-204. 2005.... - N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatBPetra Van Damme
Department of Medical Protein Research, Vlaams Instituut voor Biotechnologie, Ghent University, B 9000 Ghent, Belgium
Proc Natl Acad Sci U S A 109:12449-54. 2012.... - Proteome-wide characterization of N-glycosylation events by diagonal chromatographyBart Ghesquière
Department of Biochemistry and Medical Protein Research, Faculty of Medicine and Health Sciences, Ghent University, A Baertsoenkaai 3, B9000 Ghent, Belgium
J Proteome Res 5:2438-47. 2006..These properties--high sensitivity and dependence on glycan imprinting--can be exploited for glycan-biomarker analysis... - Improved tandem mass spectrometric characterization of 3-nitrotyrosine sites in peptidesBart Ghesquière
Department of Medical Protein Research and Biochemistry, Flanders Interuniversity Institute for Biotechnology and Faculty of Medicine and Health Sciences, Ghent University, A Baertsoenkaai 3, B 9000 Ghent, Belgium
Rapid Commun Mass Spectrom 20:2885-93. 2006..In conclusion, we here illustrate that this two-step process, heme-mediated reduction and acetylation, can be used for more efficient characterization of protein-bound nitrated tyrosines... - Protein processing and other modifications analyzed by diagonal peptide chromatographyKris Gevaert
Department of Medical Protein Research and Biochemistry, Flanders Interuniversity Institute for Biotechnology and Faculty of Medicine and Health Sciences, Ghent University, A Baertsoenkaai 3, B 9000 Ghent, Belgium
Biochim Biophys Acta 1764:1801-10. 2006..Additional modifications that can be analyzed include phosphorylation and N-glycosylation. The potential of COFRADIC for isolating peptides holding such modified amino acids are discussed here... - Stable isotopic labeling in proteomicsKris Gevaert
Department of Medical Protein Research, VIB, Ghent University, Ghent, Belgium
Proteomics 8:4873-85. 2008..In this review article, commonly used isotope labeling strategies are described, both for quantitative differential protein profiling and for targeted analysis of protein modifications... - Peptizer, a tool for assessing false positive peptide identifications and manually validating selected resultsKenny Helsens
Department of Medical Protein Research, VIB, B 9000 Ghent, Belgium
Mol Cell Proteomics 7:2364-72. 2008..Peptizer is open source software under the Apache2 license and is written in Java... - ms_lims, a simple yet powerful open source laboratory information management system for MS-driven proteomicsKenny Helsens
Department of Medical Protein Research, VIB, Ghent, Belgium
Proteomics 10:1261-4. 2010..We here present ms_lims (http://genesis.UGent.be/ms_lims), a freely available, open-source system based on a central database to automate data management and processing in MS-driven proteomics analyses... - Hybrid N-glycans on the host protective activation-associated secreted proteins of Ostertagia ostertagi and their importance in immunogenicityYves Meyvis
Laboratory of Parasitology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B 9820 Merelbeke, Belgium
Mol Biochem Parasitol 161:67-71. 2008..While removal of these glycans had little effect on antibody recognition by vaccinated animals, denaturing and reducing the proteins dramatically reduced recognition, suggesting the importance of conformational protein backbone epitopes... - Complementary positional proteomics for screening substrates of endo- and exoproteasesPetra Van Damme
Department of Medical Protein Research, Vlaams Instituut voor Biotechnologie, Ghent, Belgium
Nat Methods 7:512-5. 2010.... - A quantitative proteomics design for systematic identification of protease cleavage eventsFrancis Impens
Department of Medical Protein Research, VIB, B 9000 Ghent, Belgium
Mol Cell Proteomics 9:2327-33. 2010.... - Four stage liquid chromatographic selection of methionyl peptides for peptide-centric proteome analysis: the proteome of human multipotent adult progenitor cellsKris Gevaert
Department of Biochemistry and Medical Protein Research, Faculty of Medicine and Health Sciences, Ghent University, A Baertsoenkaai 3, B9000 Ghent, Belgium
J Proteome Res 5:1415-28. 2006.... - Global phosphoproteome analysis on human HepG2 hepatocytes using reversed-phase diagonal LCKris Gevaert
Department of Medical Protein Research, Flanders Interuniversity Institute for Biotechnology, Ghent University, Ghent, Belgium
Proteomics 5:3589-99. 2005..The method was validated with alpha-casein phosphopeptides and consecutively applied on HepG2 cells. We identified 190 phosphorylated peptides from 152 different proteins. This dataset includes 38 novel protein phosphorylation sites... - A comparison of MS2-based label-free quantitative proteomic techniques with regards to accuracy and precisionNiklaas Colaert
Department of Medical Protein Research, VIB, Ghent, Belgium
Proteomics 11:1110-3. 2011..We derived our test data from two well-characterized publicly available quantitative data sets... - The C-terminus of CIS defines its interaction patternDelphine Lavens
Flanders Interuniversity Institute for Biotechnology, Department of Medical Protein Research VIB09, Faculty of Medicine and Health Sciences, Ghent University, Baertsoenkaai 3, 9000 Ghent, Belgium
Biochem J 401:257-67. 2007..Mutagenesis revealed that one single tyrosine residue at position 253 is a critical binding determinant. In contrast, substrate binding by the highly related SOCS2 protein, and also by SOCS1 and SOCS3, does not require their SOCS-box... - A reproducibility-based evaluation procedure for quantifying the differences between MS/MS peak intensity normalization methodsSven Degroeve
Department of Medical Protein Research, VIB, Ghent, Belgium
Proteomics 11:1172-80. 2011..We demonstrate that our results are independent of the data set used in the evaluation procedure, allowing us to provide generic guidance on the choice of normalization method to apply in a certain MS/MS pipeline application... - Thermo-msf-parser: an open source Java library to parse and visualize Thermo Proteome Discoverer msf filesNiklaas Colaert
Department of Medical Protein Research, VIB, Ghent, Belgium
J Proteome Res 10:3840-3. 2011..All code, binaries, and documentation is freely available at http://thermo-msf-parser.googlecode.com... - The Online Protein Processing Resource (TOPPR): a database and analysis platform for protein processing eventsNiklaas Colaert
Department of Medical Protein Research, VIB, Ghent University, A Baertsoenkaai 3, B 9000 Ghent, Belgium
Nucleic Acids Res 41:D333-7. 2013..Across substrates, substrate orthologs and conserved sequence stretches can also be shown, with iceLogo visualization provided for the latter... - Cells lacking β-actin are genetically reprogrammed and maintain conditional migratory capacityDavina Tondeleir
Department of Medical Protein Research, VIB, Ghent, Belgium
Mol Cell Proteomics 11:255-71. 2012.... - A case study on the comparison of different software tools for automated quantification of peptidesNiklaas Colaert
Department of Medical Protein Research, VIB, Ghent University, B 9000, Ghent, Belgium
Methods Mol Biol 753:373-98. 2011..Recently, new tools were developed for automatic peptide quantification in quantitative proteomic experiments. Here we present a case study where the most recent and frequently used tools are analyzed and compared... - Protein processing characterized by a gel-free proteomics approachPetra Van Damme
Ghent University, Ghent, Belgium
Methods Mol Biol 484:245-62. 2008..As such, our gel-free proteomics technology is widely applicable and amenable for a variety of proteome-driven protease degradomics research... - RIBAR and xRIBAR: Methods for reproducible relative MS/MS-based label-free protein quantificationNiklaas Colaert
Department of Medical Protein Research, VIB, B 9000 Ghent, Belgium
J Proteome Res 10:3183-9. 2011..Both the accuracy and the precision of our novel methods are analyzed and compared to the existing methods to illustrate the increased robustness of our new methods over existing ones... - HyperISGylation of Old World monkey ISG15 in human cellsEls Pattyn
Department of Medical Protein Research, VIB, Ghent, Belgium
PLoS ONE 3:e2427. 2008..Upon the cooperative activity of specific Ubiquitinating enzymes, ISG15 can be conjugated to its substrates. Increasing evidence points to a role for protein ISGylation in anti-viral and anti-tumoral defense... - Analysis of the translationally controlled tumour protein in the nematodes Ostertagia ostertagi and Caenorhabditis elegans suggests a pivotal role in egg productionYves Meyvis
Laboratory of Parasitology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B 9820 Merelbeke, Belgium
Int J Parasitol 39:1205-13. 2009..elegans. Knock-down of C. elegans tctp (tct-1) transcription reduced the numbers of eggs laid by the hermaphrodite in the F(0) and F(1) generations by 90% and 72%, respectively, indicating a pivotal role of TCTP in reproduction... - Angiotensin I-converting enzyme inhibitory activity of gelatin hydrolysates and identification of bioactive peptidesGriet Herregods
Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, B 9000 Ghent, Belgium
J Agric Food Chem 59:552-8. 2011..As these peptides showed ACE inhibitory activity and were more prominent in Bh2t than in Bh2, the current data provide evidence that these contribute to the antihypertensive effect of Bh2t... - Global differential non-gel proteomics by quantitative and stable labeling of tryptic peptides with oxygen-18An Staes
Department of Medical Protein Research, Faculty of Medicine and Health Sciences, Ghent University, A Baertsoenkaai 3, B-9000 Ghent, Belgium
J Proteome Res 3:786-91. 2004.... - Reverse-phase diagonal chromatography for phosphoproteome researchKris Gevaert
Department of Medical Protein Research, Ghent University, Ghent, Belgium
Methods Mol Biol 527:219-27, ix. 2009..The complete COFRADIC sorting procedure is described here... - A comparison of the HUPO Brain Proteome Project pilot with other proteomics studiesLennart Martens
Department of Biochemistry and Medical Protein Research, Faculty of Medicine and Health Sciences, Flanders Interuniversity Institute for Biotechnology, Ghent University, Ghent, Belgium
Proteomics 6:5076-86. 2006..Finally, as any comparison between large-scale proteomics datasets is decidedly non-trivial, we also evaluate and discuss several ways to go about comparing such different result sets... - The tandem PDZ protein Syntenin interacts with the aminoacyl tRNA synthetase complex in a lysyl-tRNA synthetase-dependent mannerKris Meerschaert
Department of Medical Protein Research, VIB, B 9000 Ghent, Belgium
J Proteome Res 7:4962-73. 2008..As a result, other components of the ARS complex no longer co-immunoprecipitate with syntenin-1. We further show that syntenin-1 regulates KRS activity. These findings suggest that syntenin-1 is an adaptor modulating the activity of KRS... - Mass spectrometry-driven proteomics: an introductionKenny Helsens
Department of Medical Protein Research, VIB, Ghent University, B 9000, Ghent, Belgium
Methods Mol Biol 753:1-27. 2011..To accomplish this, many methods have been developed over the last decades, and their general principles and important recent advances in large-scale protein analysis or proteomics are discussed in this review... - Protein alpha-N-acetylation studied by N-terminomicsPetra Van Damme
Department of Medical Protein Research, VIB, Ghent, Belgium
FEBS J 278:3822-34. 2011..In this review, we report on such emerging technologies as well as on breakthroughs in our understanding of protein N-terminal biology... - DBToolkit: processing protein databases for peptide-centric proteomicsLennart Martens
Department of Biochemistry, Faculty of Medicine and Health Sciences, Ghent University, A Baertsoenkaai 3, B 9000 Ghent, Belgium
Bioinformatics 21:3584-5. 2005..The source code, full user and developer documentation and cross-platform binaries are freely downloadable from the project website at http://genesis.UGent.be/dbtoolkit/ CONTACT: lennart.martens@UGent.be.. - Diagonal reverse-phase chromatography applications in peptide-centric proteomics: ahead of catalogue-omics?Kris Gevaert
Department of Medical Protein Research, Flanders Interuniversity Institute for Biotechnology, Department of Biochemistry, Ghent University, A Baertsoenkaai 3, B 9000 Ghent, Belgium
Anal Biochem 345:18-29. 2005..We were able to identify an unexpectedly high number of intracellular proteins next to known biomarkers... - MascotDatfile: an open-source library to fully parse and analyse MASCOT MS/MS search resultsKenny Helsens
Department of Biochemistry and Medical Protein Research, Faculty of Medicine and Health Sciences, Flanders Interuniversity Institute for Biotechnology, Ghent University, Ghent, Belgium
Proteomics 7:364-6. 2007..ugent.be/MascotDatfile). Several scripts based on this library are provided as examples, allowing direct automation of important routine tasks involved in processing peptide identifications... - A review of COFRADIC techniques targeting protein N-terminal acetylationPetra Van Damme
Department of Medical Protein Research, VIB, B 9000 Ghent, Belgium
BMC Proc 3:S6. 2009.... - Multiple isoforms of the tumor suppressor myopodin are simultaneously transcribed in cancer cellsAriane De Ganck
Department of Medical Protein Research, VIB, B 9000 Ghent, Belgium
Biochem Biophys Res Commun 370:269-73. 2008..These findings shed new light on the expression of this tumor suppressor gene and necessitate closer examination of both mouse and human myopodin polypeptides currently under study... - Who gets cut during cell death?Francis Impens
Department of Medical Protein Research, VIB, B 9000 Ghent, Belgium
Curr Opin Cell Biol 22:859-64. 2010..This review provides an overview of the results obtained at the exciting interface between proteomics, protease biology and cell death... - Glycosylation signatures in Drosophila: fishing with lectinsGianni Vandenborre
Laboratory of Agrozoology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, Ghent, Belgium
J Proteome Res 9:3235-42. 2010..Furthermore, it was demonstrated that a specific glycosylation signature can be associated with a functionally related group of glycoproteins in Drosophila, both in terms of biological process and molecular function... - A small heat shock protein of Ostertagia ostertagi: stage-specific expression, heat inducibility, and protection trialIsabel Vercauteren
Laboratory of Parasitology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan, 133, B-9820 Merelbeke, Belgium
J Parasitol 92:1244-50. 2006..The levels of Oo-hsp18 mRNAs are sharply induced by heat shock but not by other stressors such as levamisole and H2O2. A vaccination trial with recombinant Oo-HSP18 failed to protect calves against a challenge infection... - Reversible labeling of cysteine-containing peptides allows their specific chromatographic isolation for non-gel proteome studiesKris Gevaert
Department of Biochemistry, Faculty of Medicine and Health Sciences, Ghent University and Flanders Interuniversity Institute for Biotechnology, Ghent, Belgium
Proteomics 4:897-908. 2004..In both proteomes, a significant number of low abundance proteins were identified next to extremely abundant ones. A dynamic range for protein identification spanning 4-5 orders of magnitude is demonstrated... - Comparative proteome analysis of Chlamydia trachomatis serovar A, D and L2Allan C Shaw
Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus, Denmark
Proteomics 2:164-86. 2002..trachomatis on a large scale and the integration of our data in a 2-D PAGE database will create a basis for post genomic research, important for the understanding of chlamydial development and pathogenesis... - Proteome-wide Identification of HtrA2/Omi SubstratesLieselotte Vande Walle
Department of Molecular Biomedical Research, VIB, 9052, Ghent, Belgium
J Proteome Res 6:1006-15. 2007..Our results suggest that HtrA2/Omi has a rather narrow cleavage site preference and that cytoskeletal proteins are prime targets of this protease... - Chromatographic isolation of methionine-containing peptides for gel-free proteome analysis: identification of more than 800 Escherichia coli proteinsKris Gevaert
Department of Biochemistry, Faculty of Medicine and Health Sciences, Ghent University and Flanders Interuniversity Institute for Biotechnology, Belgium
Mol Cell Proteomics 1:896-903. 2002..adding a new dimension to future proteome research... - Abolition of stress-induced protein synthesis sensitizes leukemia cells to anthracycline-induced deathGro Gausdal
Department of Biomedicine, University of Bergen, Jonas Lies vei 91, Bergen, Norway
Blood 111:2866-77. 2008..We propose that judiciously timed inhibition of cap-independent translation is considered for combination therapy with anthracyclines in AML... - Human Proteinpedia enables sharing of human protein dataSuresh Mathivanan
Nat Biotechnol 26:164-7. 2008 - Improving the reliability and throughput of mass spectrometry-based proteomics by spectrum quality filteringKristian Flikka
Computational Biology Unit, Bergen Center for Computational Science, Bergen, Norway
Proteomics 6:2086-94. 2006..The implementation of the classifiers is available under the GNU General Public License at http://www.bioinfo.no/software/spectrumquality... - The proteome of the human neuroblastoma cell line SH-SY5Y: an enlarged proteomeKambiz Gilany
Department of Biomedical Sciences, Protein Chemistery, University of Antwerp, Universiteitsplein 1, B 2610 Antwerp, Belgium
Biochim Biophys Acta 1784:983-5. 2008..The obtained proteome profile of this cell line is discussed... - Large-scale identification of N-terminal peptides in the halophilic archaea Halobacterium salinarum and Natronomonas pharaonisMichalis Aivaliotis
Department of Membrane Biochemistry, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany
J Proteome Res 6:2195-204. 2007..The protein sets described in this paper are available by FTP and might be used as reference sets to test the performance of new gene finders... - Identification by two-dimensional electrophoresis of a new adhesin expressed by a low-passaged strain of Mycoplasma bovisAnne Thomas
Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liege, B43A, Sart Tilman, 4000 Liege, Belgium
Res Microbiol 156:713-8. 2005..Serum from a mouse immunized with these proteins significantly reduced adherence of M. bovis to BBE cells. This result underlines the function of this new Vsp in adherence of M. bovis to host cells...