W Fiers

Summary

Affiliation: Ghent University
Country: Belgium

Publications

  1. ncbi A "universal" human influenza A vaccine
    W Fiers
    Laboratory Molecular Biology, University Ghent and VIB, Ledeganckstreet 35, 9000 Ghent, Belgium
    Virus Res 103:173-6. 2004
  2. pmc Soluble recombinant influenza vaccines
    W Fiers
    Laboratory of Molecular Biology, University of Ghent and Flanders Interuniversity Institute for Biotechnology, K L Ledeganckstraat 35, B 9000 Ghent, Belgium
    Philos Trans R Soc Lond B Biol Sci 356:1961-3. 2001
  3. pmc The p38/RK mitogen-activated protein kinase pathway regulates interleukin-6 synthesis response to tumor necrosis factor
    R Beyaert
    Laboratory of Molecular Biology, University of Ghent, Belgium
    EMBO J 15:1914-23. 1996
  4. ncbi A20, an inhibitor of cell death, self-associates by its zinc finger domain
    D De Valck
    Laboratory of Molecular Biology, University of Gent, Belgium
    FEBS Lett 384:61-4. 1996
  5. ncbi Identification of a locus on distal mouse chromosome 12 that controls resistance to tumor necrosis factor-induced lethal shock
    C Libert
    Department of Molecular Biology, Flanders Interuniversity Institute for Biotechnology and University of Ghent, Belgium
    Genomics 55:284-9. 1999
  6. ncbi Tight transcriptional control mechanism ensures stable high-level expression from T7 promoter-based expression plasmids
    N Mertens
    Laboratory of Molecular Biology, University of Gent, Belgium
    Biotechnology (N Y) 13:175-9. 1995
  7. ncbi An antibody which binds to the membrane-proximal end of influenza virus haemagglutinin (H3 subtype) inhibits the low-pH-induced conformational change and cell-cell fusion but does not neutralize virus
    P Vanlandschoot
    Division of Virology, MRC, National Institute for Medical Research, London, UK
    J Gen Virol 79:1781-91. 1998
  8. ncbi High-level constitutive expression of alpha 1-acid glycoprotein and lack of protection against tumor necrosis factor-induced lethal shock in transgenic mice
    C Libert
    Flanders Interuniversity Institute for Biotechnology, Ghent, Belgium
    Transgenic Res 7:429-35. 1998
  9. pmc The zinc finger protein A20 inhibits TNF-induced NF-kappaB-dependent gene expression by interfering with an RIP- or TRAF2-mediated transactivation signal and directly binds to a novel NF-kappaB-inhibiting protein ABIN
    K Heyninck
    Department of Molecular Biology, Flanders Interuniversity Institute for Biotechnology, University of Ghent, B 9000 Ghent, Belgium
    J Cell Biol 145:1471-82. 1999
  10. ncbi Tumor necrosis factor-induced microtubule stabilization mediated by hyperphosphorylated oncoprotein 18 promotes cell death
    K Vancompernolle
    Department of Molecular Biology, Ghent University and Flanders Interuniversity Institute for Biotechnology, K L Ledeganckstraat 35, B 9000 Ghent, Belgium
    J Biol Chem 275:33876-82. 2000

Collaborators

  • P Cohen
  • N Takahashi
  • J R Kinghorn
  • P Huang
  • L Steidler
  • J C Lee
  • C Libert
  • A Cuenda
  • R Willemze
  • K Vancompernolle
  • Rosemary Elliott
  • D Burger
  • J Tavernier
  • Jean Michel Dayer
  • Anje Cauwels
  • R Beyaert
  • P Vandenabeele
  • M Van de Craen
  • W Declercq
  • P Ameloot
  • G van Loo
  • D De Valck
  • G Denecker
  • K Heyninck
  • W Van Criekinge
  • G Haegeman
  • D Vercammen
  • P Brouckaert
  • R Contreras
  • J Grooten
  • I Van den Brande
  • G De Wilde
  • N Mertens
  • K J Baeyens
  • E Decoster
  • F Molemans
  • P Vanlandschoot
  • P Schotte
  • W Vanden Berghe
  • R M Barge
  • R Lucas
  • W A Buurman
  • A Marchant
  • B Vanhaesebroeck
  • T Vanden Berghe
  • P De Bleser
  • C F Ware
  • G Brouckaert
  • M Steemans
  • E Boone
  • B De Vreese
  • B Zhivotovsky
  • J Van Beeumen
  • A Raeymaekers
  • K T Jeang
  • H L De Bondt
  • C J De Ranter
  • D Y Jin
  • W M Jou
  • B Barrere
  • V Goossens
  • S Wharton
  • B Everaerdt
  • S Pype
  • S Mandruzzato
  • L Calder
  • P van der Bruggen
  • E Beirnaert
  • B Millar
  • P Juillard
  • R Gururajan
  • V J Kidd
  • H Bluethmann
  • T De Kesel
  • Y Donati
  • G E Grau
  • J M Lahti
  • S Cornelis
  • M Redard
  • C Giroud
  • C Monso-Hinard
  • M W Moore
  • Z Amraoui
  • O Le Moine
  • M Goldman
  • S Plaisance
  • C Gueydan
  • C Bruyns
  • J R Vandenheede
  • J van Lint

Detail Information

Publications41

  1. ncbi A "universal" human influenza A vaccine
    W Fiers
    Laboratory Molecular Biology, University Ghent and VIB, Ledeganckstreet 35, 9000 Ghent, Belgium
    Virus Res 103:173-6. 2004
    ..Appropriate combinations of vaccine construct and adjuvant allowed to obtain anti-M2e IgG2a serum titers above 10,000, and this provided complete protection...
  2. pmc Soluble recombinant influenza vaccines
    W Fiers
    Laboratory of Molecular Biology, University of Ghent and Flanders Interuniversity Institute for Biotechnology, K L Ledeganckstraat 35, B 9000 Ghent, Belgium
    Philos Trans R Soc Lond B Biol Sci 356:1961-3. 2001
    ..The potential of the resulting protein as a vaccine candidate remains to be evaluated...
  3. pmc The p38/RK mitogen-activated protein kinase pathway regulates interleukin-6 synthesis response to tumor necrosis factor
    R Beyaert
    Laboratory of Molecular Biology, University of Ghent, Belgium
    EMBO J 15:1914-23. 1996
    ..Interestingly, TNF-induced cytotoxicity was not affected by SB203580, indicating that p38 MAP kinase might be an interesting target to interfere selectively with TNF-induced gene activation...
  4. ncbi A20, an inhibitor of cell death, self-associates by its zinc finger domain
    D De Valck
    Laboratory of Molecular Biology, University of Gent, Belgium
    FEBS Lett 384:61-4. 1996
    ..Here we report that A20 is able to self-associate, and demonstrate that the latter interaction is mediated by its zinc finger domain...
  5. ncbi Identification of a locus on distal mouse chromosome 12 that controls resistance to tumor necrosis factor-induced lethal shock
    C Libert
    Department of Molecular Biology, Flanders Interuniversity Institute for Biotechnology and University of Ghent, Belgium
    Genomics 55:284-9. 1999
    ..This study also demonstrates that loci affecting important biological responses can be identified with very high resolution using recombinant inbred mice...
  6. ncbi Tight transcriptional control mechanism ensures stable high-level expression from T7 promoter-based expression plasmids
    N Mertens
    Laboratory of Molecular Biology, University of Gent, Belgium
    Biotechnology (N Y) 13:175-9. 1995
    ..The applicability of the system is illustrated by the strictly regulatable, high-level production of several cytokines of human and murine origin...
  7. ncbi An antibody which binds to the membrane-proximal end of influenza virus haemagglutinin (H3 subtype) inhibits the low-pH-induced conformational change and cell-cell fusion but does not neutralize virus
    P Vanlandschoot
    Division of Virology, MRC, National Institute for Medical Research, London, UK
    J Gen Virol 79:1781-91. 1998
    ..However, LMBH6 cannot prevent infection of MDCK cells but slows the growth of virus when included in a plaque assay overlay...
  8. ncbi High-level constitutive expression of alpha 1-acid glycoprotein and lack of protection against tumor necrosis factor-induced lethal shock in transgenic mice
    C Libert
    Flanders Interuniversity Institute for Biotechnology, Ghent, Belgium
    Transgenic Res 7:429-35. 1998
    ..This study provides an interesting example of differences in outcome to a lethal challenge between an acute administered and a chronically produced protective protein...
  9. pmc The zinc finger protein A20 inhibits TNF-induced NF-kappaB-dependent gene expression by interfering with an RIP- or TRAF2-mediated transactivation signal and directly binds to a novel NF-kappaB-inhibiting protein ABIN
    K Heyninck
    Department of Molecular Biology, Flanders Interuniversity Institute for Biotechnology, University of Ghent, B 9000 Ghent, Belgium
    J Cell Biol 145:1471-82. 1999
    ....
  10. ncbi Tumor necrosis factor-induced microtubule stabilization mediated by hyperphosphorylated oncoprotein 18 promotes cell death
    K Vancompernolle
    Department of Molecular Biology, Ghent University and Flanders Interuniversity Institute for Biotechnology, K L Ledeganckstraat 35, B 9000 Ghent, Belgium
    J Biol Chem 275:33876-82. 2000
    ..The data suggest that Op18 and the MT network play a functional role in transduction of the cell death signal to the mitochondria...
  11. ncbi Treatment of murine colitis by Lactococcus lactis secreting interleukin-10
    L Steidler
    Department of Molecular Biology, Ghent University and Flanders Interuniversity Institute for Biotechnology, K L Ledeganckstraat 35, 9000 Gent, Belgium
    Science 289:1352-5. 2000
    ..This approach may lead to better methods for cost-effective and long-term management of IBD in humans...
  12. ncbi Protection against TNF-induced lethal shock by soluble guanylate cyclase inhibition requires functional inducible nitric oxide synthase
    A Cauwels
    MPET, Department of Molecular Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, Belgium
    Immunity 13:223-31. 2000
    ..These data imply that iNOS- (and not eNOS-) derived NO is an endogenous protective molecule indispensable to survive a TNF challenge and exerting this beneficial effect via sGC-independent mechanisms...
  13. ncbi Recombinant IL-1 receptor antagonist protects against TNF-induced lethality in mice
    B Everaerdt
    Laboratory of Molecular Biology, University, Gent, Belgium
    J Immunol 152:5041-9. 1994
    ..Furthermore, the increased susceptibility of tumor-bearing mice to human TNF, relative to control mice, or the sensitization by co-administration of RU38486 or D(+)-galactosamine do not seem to be mediated by IL-1...
  14. pmc Protection by alpha 1-acid glycoprotein against tumor necrosis factor-induced lethality
    C Libert
    Laboratory of Molecular Biology, University of Gent, Belgium
    J Exp Med 180:1571-5. 1994
    ..The known platelet aggregation-inhibitory activity of alpha 1-acid glycoprotein provides a possible explanation for this protective capacity...
  15. ncbi Generation and biological characterization of membrane-bound, uncleavable murine tumor necrosis factor
    E Decoster
    Laboratory of Molecular Biology, Gent University, Belgium
    J Biol Chem 270:18473-8. 1995
    ..This indicates that membrane-bound TNF cannot be passed from the 75-kDa to the 55-kDa TNF receptor...
  16. ncbi Expression in non-lymphoid cells of mouse recombinant immunoglobulin directed against the tumour marker human placental alkaline phosphatase
    P de Waele
    Laboratory of Molecular Biology, State University of Gent, Belgium
    Eur J Biochem 176:287-95. 1988
    ..This illustrates that non-lymphoid cells also have the capacity to assemble active immunoglobulins...
  17. ncbi Response of interleukin-6-deficient mice to tumor necrosis factor-induced metabolic changes and lethality
    C Libert
    Laboratory of Molecular Biology, University, Gent, Belgium
    Eur J Immunol 24:2237-42. 1994
    ..The results indicate that IL-6 is not contributing in a major way to the pathogenesis leading to TNF-induced shock, and that neither IL-6 nor the APP studied are essential for a protective feedback system...
  18. ncbi Cleavage of PITSLRE kinases by ICE/CASP-1 and CPP32/CASP-3 during apoptosis induced by tumor necrosis factor
    R Beyaert
    Laboratory of Molecular Biology, Flanders Interuniversity Institute for Biotechnology and University of Ghent, B 9000 Ghent, Belgium
    J Biol Chem 272:11694-7. 1997
    ..These data implicate CASP-3 as a potentially important CASP family protease responsible for the cleavage of PITSLRE kinases during TNF-induced apoptosis...
  19. ncbi TNF-mediated IL6 gene expression and cytotoxicity are co-inducible in TNF-resistant L929 cells
    V Vandevoorde
    Laboratory of Molecular Biology, Gent University, Belgium
    FEBS Lett 302:235-8. 1992
    ..These results confirm our earlier conclusions regarding the close relationship between TNF-mediated IL6 gene expression and the pathway leading to cytotoxicity...
  20. ncbi Efficient KEX2-like processing of a glucoamylase-interleukin-6 fusion protein by Aspergillus nidulans and secretion of mature interleukin-6
    R Contreras
    Laboratory of Molecular Biology, State University, Gent, Belgium
    Biotechnology (N Y) 9:378-81. 1991
    ..The KEX2-type processing signal, Lys-Arg, is recognized and cleaved efficiently by an enzyme present in A. nidulans resulting in the secretion of an authentic mature hIL-6 protein at levels of up to 5 mg/l...
  21. ncbi Identification of tumor necrosis factor (TNF) amino acids crucial for binding to the murine p75 TNF receptor and construction of receptor-selective mutants
    P Ameloot
    Department of Molecular Biology, Flanders Interuniversity Institute for Biotechnology and Ghent University, 9000 Ghent, Belgium
    J Biol Chem 276:37426-30. 2001
    ..In conclusion, the mutation of TNF amino acids 71-73, 89, and 102 is sufficient to obtain a mTNF mutein selective for mTNF-RI and a hTNF mutein that, unlike wild-type hTNF, also acts on mTNF-RII...
  22. ncbi Casein kinase-1 phosphorylates the p75 tumor necrosis factor receptor and negatively regulates tumor necrosis factor signaling for apoptosis
    R Beyaert
    Laboratory of Molecular Biology, Flemish Institute for Biotechnology, University of Ghent, Belgium
    J Biol Chem 270:23293-9. 1995
    ..Taken together, our data demonstrate that the p75 TNF receptor is phosphorylated and associated with CK-1, which negatively regulates p75-mediated TNF signaling...
  23. ncbi Molecular mechanisms of tumor necrosis factor-induced cytotoxicity. What we do understand and what we do not
    R Beyaert
    Laboratory of Molecular Biology, Gent University, Belgium
    FEBS Lett 340:9-16. 1994
    ..Here we review TNF-induced post-receptor signaling mechanisms which seem to be involved in the pathway to cytotoxicity...
  24. ncbi Heterotrimers formed by tumor necrosis factors of different species or muteins
    P Ameloot
    Department of Molecular Biology, Unit of Molecular Pathophysiology and Experimental Therapy, and Unit of Molecular Signaling and Cell Death, Flanders Interuniversity Institute for Biotechnology and Ghent University, 9000 Ghent, Belgium
    J Biol Chem 276:27098-103. 2001
    ....
  25. ncbi Molecular cloning and identification of murine caspase-8
    M Van de Craen
    Department of Molecular Biology, Flanders Interuniversity Institute for Biotechnology and University of Ghent, K L Ledeganckstraat 35, Ghent, B 9000, Belgium
    J Mol Biol 284:1017-26. 1998
    ..In addition, murine caspase-8 induced cell death that was inhibited both by cytokine response modifier A and p35. In vitro experiments demonstrated that p35 inhibits caspase-8 directly...
  26. pmc Localization of the active site of human tumour necrosis factor (hTNF) by mutational analysis
    X Van Ostade
    Laboratory of Molecular Biology, State University of Ghent, Belgium
    EMBO J 10:827-36. 1991
    ..Our results favor a model in which each TNF molecule has three receptor-interaction sites (between the three subunits), thus allowing signal transmission by receptor clustering...
  27. pmc Inhibition of caspases increases the sensitivity of L929 cells to necrosis mediated by tumor necrosis factor
    D Vercammen
    Laboratory of Molecular Biology, Flanders Interuniversity Institute for Biotechnology and University of Ghent, B 9000 Ghent, Belgium
    J Exp Med 187:1477-85. 1998
    ..These results indicate an involvement of caspases in protection against TNF-induced formation of oxygen radicals and necrosis...
  28. ncbi Differential involvement of caspases in apoptosis of myeloid leukemic cells induced by chemotherapy versus growth factor withdrawal
    R M Barge
    Laboratory of Molecular Biology, Flanders Interuniversity Institute for Biotechnology, University of Gent, Belgium
    FEBS Lett 409:207-10. 1997
    ..In contrast, DEVD-fmk as well as ZVAD-fmk protect 32D cells from ActD-induced apoptosis. These results indicate that different caspases are involved in apoptosis induced by growth factor withdrawal and by chemotherapy...
  29. ncbi Modulation of cellular susceptibility to the cytotoxic/cytostatic action of tumor necrosis factor by adenovirus E1 gene expression is cell type-dependent
    B Vanhaesebroeck
    Laboratory of Molecular Biology, State University, Gent, Belgium
    Virology 176:362-8. 1990
    ..Differences in E1A expression levels between cell types cannot explain this discrepancy regarding modulation of TNF sensitivity by E1A...
  30. ncbi Death receptor-induced apoptotic and necrotic cell death: differential role of caspases and mitochondria
    G Denecker
    Molecular Signaling and Cell Death Unit, Department of Molecular Biology, Flanders Interuniversity Institute for Biotechnology and Ghent University, 9000 Gent, Belgium
    Cell Death Differ 8:829-40. 2001
    ....
  31. ncbi The zinc finger protein A20 interacts with a novel anti-apoptotic protein which is cleaved by specific caspases
    D De Valck
    Department of Molecular Biology, Flanders Interuniversity Institute for Biotechnology and University of Gent, Belgium
    Oncogene 18:4182-90. 1999
    ..caspase-3, caspase-6 and caspase-7. Thus TXBP151 appears to be a novel A20-binding protein which might mediate the anti-apoptotic activity of A20, and which can be processed by specific caspases...
  32. ncbi A human high affinity interleukin-5 receptor (IL5R) is composed of an IL5-specific alpha chain and a beta chain shared with the receptor for GM-CSF
    J Tavernier
    Roche Research Gent, Belgium
    Cell 66:1175-84. 1991
    ....
  33. ncbi Expression in Escherichia coli of the death domain of the human p55 tumor necrosis factor receptor
    G De Wilde
    Department of Molecular Biology, Gent University and Flanders Interuniversity Institute for Biotechnology, 9000 Gent, Belgium
    Protein Expr Purif 23:226-32. 2001
    ..We conclude that TRX-DD is correctly folded and can be used for further structure/function analysis...
  34. pmc Genes for the tumor necrosis factors alpha and beta are linked to the human major histocompatibility complex
    T Spies
    Proc Natl Acad Sci U S A 83:8699-702. 1986
    ..This assignment is based on Southern blot analysis of a number of different MHC deletion mutants and is corroborated by chromosome in situ hybridization...
  35. ncbi Identification of a new caspase homologue: caspase-14
    M Van de Craen
    Department of Molecular Biology, Flanders Interuniversity Institute for Biotechnology and University of Ghent, Ghent, Belgium
    Cell Death Differ 5:838-46. 1998
    ..The high constitutive expression levels in embryos and specific expression in adult skin suggest a role in ontogenesis and skin physiology...
  36. ncbi The structure of mouse tumour-necrosis factor at 1.4 A resolution: towards modulation of its selectivity and trimerization
    K J Baeyens
    Laboratorium voor Analytische Chemie en Medicinale Fysicochemie, E Van Evenstraat 4, B 3000 Leuven, Belgium
    Acta Crystallogr D Biol Crystallogr 55:772-8. 1999
    ..Compared with human TNFs, the channel formed by the three monomers in mTNF is narrower. One 2-propanol molecule trapped in the trimeric channel could be a lead compound for the design of TNF inhibitors...
  37. ncbi Crucial role of tumor necrosis factor (TNF) receptor 2 and membrane-bound TNF in experimental cerebral malaria
    R Lucas
    Department of Anesthesiology, Pharmacology and Surgical Intensive Care, University Medical Center, Geneva, Switzerland
    Eur J Immunol 27:1719-25. 1997
    ..In conclusion, these results indicate that the interaction between membrane TNF and TNFR2 is crucial in the development of this neurological syndrome...
  38. ncbi Complete nucleotide sequence of the nucleoprotein gene from the human influenza strain A/PR/8/34 (HON1)
    L van Rompuy
    Eur J Biochem 116:347-53. 1981
    ..The described nucleoprotein structure brings the number of sequenced genes of influenza A/PR/8/34 to five out of eight genes...
  39. ncbi Characterization of seven murine caspase family members
    M Van de Craen
    Flanders Interuniversity Institute for Biotechnology and University of Ghent, Belgium
    FEBS Lett 403:61-9. 1997
    ..Only two, mCASP-1 as well as mCASP-3, were able to process precursor interleukin (IL)-1beta to biologically active IL-1beta. In addition, mCASP-3 is the predominant PARP-cleaving enzyme in vivo...
  40. pmc Methylprednisolone differentially regulates IL-10 and tumour necrosis factor (TNF) production during murine endotoxaemia
    A Marchant
    Laboratoire d Immunologie Expérimentale, Universite Libre de Bruxelles, Belgium
    Clin Exp Immunol 106:91-6. 1996
    ..We conclude that methylprednisolone differentially regulates IL-10 and TNF production induced by LPS both in vivo and in vitro at the macrophage level...
  41. ncbi A universal vaccine against influenza
    W Fiers
    Bull Mem Acad R Med Belg 161:237-9. 2006