Koen Deventer

..The results indicate that the developed method can successfully be applied as a fast and reliable method for the detection and identification of hydroxyethylstarch...

..Finally, the applicability of the developed approach for the analysis of dietary supplements was checked by the analysis of a prohormone where several impurities and/or degradation products were found...

..Analysis of 82 routine doping samples, declared positive for formoterol during routine analysis, did not exhibit concentrations which could be attributed to misuse...

..This study confirms that thiazide degradation products can be formed in urine during transport. Hence doping control laboratories shall include them into their routine testing methods as required by WADA...

..With both techniques all 26 samples were found to be positive, showing the applicability of direct injection for the analysis of diuretics...

..The study shows that 4-amino-6-chloro-1,3-benzenedisulphonamide is an important target compound for the long time detection of thiazide diuretics in urine...

..The results were compared with those obtained with the common GC-NPD method. Results show a good correlation between both methods with correlation coefficients higher than 0.95 for all analytes...

..Because stimulants are also regularly detected in nutritional (food) supplements a section on this topic is also included...

..Hence, cathine is not a good indicator to control pseudopehedrine intake. To control the (ab)use of ephedrines in sports it is recommended that WADA reintroduce a threshold for pseudoephedrine...

..Finally, the described method was applied to the analysis of urine samples previously found to contain narcotics by our routine gas chromatography/mass spectrometry (GC/MS) method...

..8 to 16.4% for budesonide. After administration of 200microg of budesonide to five healthy volunteers budesonide could not be detected in any urine sample whereas 16alpha-hydroxyprednisolone was detectable up to 12h post-administration...

..The total run time was 15 min. All compounds were analysed in MS2 or MS3. The detection limit for all compounds was lower than 25 ng/mL except for chlorphentermine (detection limit: 250 ng/mL)...

..The excretion of carvedilol was followed after intake of one tablet of Dimitone. Other doping agents including strychnine, norbuprenorphine and mesocarb hydroxysulfate could also be detected with this method...

..The analytical run time was 11 min. The LOD for all compounds varied between 1 and 10 ng/mL. Left-over A samples, which were declared positive by GC/MS for the presence of 3'-hydroxystanozolol, were assessed using the described method...

..The metabolic nature of the unreported metabolites was also confirmed. A global strategy for the detection of steroid metabolites combining both human and chimeric mouse urine is proposed...

..6-Ene-epimethyltestosterone was found in all samples, showing its applicability in the detection of methyltestosterone misuse...

..Additionally, the intake of methandienone was confirmed by the post-target detection of a long-term metabolite...

..The presence of these compounds has also been confirmed by the analysis of five other positive fluoxymesterone urine samples...

..Limits of detection between 1 and 10 ng/ml were obtained for most analytes which fulfil current requirements. The applicability of the method was shown by analysing positive samples...

..A correlation between the steroid structure and the product ions obtained has been postulated. The application of these correlations can be useful in the elucidation of feasible structures for unknown steroids and/or their metabolites...

..Using this approach, several endogenous interferences have been elucidated in this case study. The knowledge about the structures of these interferences has been found useful to circumvent their detection in the screening method for AS...

..97. A slope close to 1 was obtained for all analytes except for AG possibly due to losses during the extraction process prior to GC-MS...

..Finally, a tentative structure for each detected metabolite has been proposed based on the product ion spectra measured with accurate masses using UPLC-QTOF MS...

..Despite the ionization limitations due to their chemical structure, most of tested anabolic steroids could be ionized using the adduct formation approach...

..Analysis of 47 routine doping samples, declared to contain salmeterol during routine analysis, did not exhibit concentrations that could be considered originating from supratherapeutic doses...

..2 ng/g. The applicability of the method was checked by the analysis of three samples from patients using T-C11. A peak for the analyte was detected in all samples with concentrations between 0.4 and 8.4 ng/g...

..Copyright © 2012 John Wiley & Sons, Ltd...

..Only after intravenous administration could desmopressin be successfully detected...

..Moreover, using a short capillary column and hydrogen as a carrier gas the run time of the method is less than 8min...

..A good correlation between the two methods was obtained (r(2) > 0.98) with a slope close to 1...

..All diuretics could be detected for periods up to 96 h after the intake of therapeutic amounts...

..The study also shows that degradation can be dependent on the urinary matrix and that the results from stability studies using stabilized, filtered, or sterilized urine can underestimate degradation effects...

..Maximum detected urinary concentrations ranged from 22.6 to 176 ng/mL. The results of this study show that the use of this type of preparation by athletes can lead to a positive doping case...

..In this work, two preparations from the black market containing an unknown MGF analogue were characterized...

..Betamethasone could be detected for up to 12 days after administration. Validation of the chromatographic separation and mass spectrometric identification of mixtures of betamethasone and dexamethasone are also presented...

..On top of that, by only changing the injection technique, the detection parameters of the IRMS are not affected and there is no loss in linearity. Copyright © 2012 John Wiley & Sons, Ltd...

..Ac-LKKTETQ was also synthesized by solid-phase peptide synthesis, and an analytical strategy for detection in plasma and urine by high-performance liquid chromatography/low resolution triple-quadrupole mass spectrometry was suggested...

..While the parent compound is predominantly excreted in conjugated form, both metabolites are solely excreted as conjugates...

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