T Coenye

Summary

Affiliation: Ghent University
Country: Belgium

Publications

  1. ncbi Classification of Centers for Disease Control Group Eugonic Fermenter (EF)-4a and EF-4b as Neisseria animaloris sp. nov. and Neisseria zoodegmatis sp. nov., respectively
    Peter Vandamme
    Laboratorium voor Microbiologie, Universiteit Gent, Gent, Belgium
    Int J Syst Evol Microbiol 56:1801-5. 2006
  2. ncbi Cinnamaldehyde and cinnamaldehyde derivatives reduce virulence in Vibrio spp. by decreasing the DNA-binding activity of the quorum sensing response regulator LuxR
    Gilles Brackman
    Laboratory of Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B 9000 Ghent, Belgium
    BMC Microbiol 8:149. 2008
  3. ncbi Phytosphingosine-1-phosphate is a signaling molecule involved in miconazole resistance in sessile Candida albicans cells
    Davy Vandenbosch
    Laboratory of Pharmaceutical Microbiology, Ghent University, Ghent, Belgium
    Antimicrob Agents Chemother 56:2290-4. 2012
  4. ncbi Eradication of Propionibacterium acnes biofilms by plant extracts and putative identification of icariin, resveratrol and salidroside as active compounds
    Tom Coenye
    Laboratory of Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B 9000 Ghent, Belgium
    Phytomedicine 19:409-12. 2012
  5. ncbi Development and evaluation of different normalization strategies for gene expression studies in Candida albicans biofilms by real-time PCR
    Heleen Nailis
    Laboratory for Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B 9000, Ghent, Belgium
    BMC Mol Biol 7:25. 2006
  6. ncbi Real-time PCR expression profiling of genes encoding potential virulence factors in Candida albicans biofilms: identification of model-dependent and -independent gene expression
    Heleen Nailis
    Laboratory for Pharmaceutical Microbiology, Universiteit Gent, Harelbekestraat 72, B 9000, Ghent, Belgium
    BMC Microbiol 10:114. 2010
  7. ncbi Transcriptional response of Burkholderia cenocepacia J2315 sessile cells to treatments with high doses of hydrogen peroxide and sodium hypochlorite
    Elke Peeters
    Laboratory of Pharmaceutical Microbiology, Ghent University, Ghent, Belgium
    BMC Genomics 11:90. 2010
  8. ncbi Extracting phylogenetic information from whole-genome sequencing projects: the lactic acid bacteria as a test case
    Tom Coenye
    Laboratorium voor Microbiologie, Universiteit Gent, K L Ledeganckstraat 35, B 9000 Gent, Belgium
    Microbiology 149:3507-17. 2003
  9. ncbi Advenella incenata gen. nov., sp. nov., a novel member of the Alcaligenaceae, isolated from various clinical samples
    Tom Coenye
    Laboratorium voor Microbiologie, Universiteit Gent, Gent, Belgium
    Int J Syst Evol Microbiol 55:251-6. 2005
  10. ncbi Organisation of the S10, spc and alpha ribosomal protein gene clusters in prokaryotic genomes
    Tom Coenye
    Laboratorium voor Microbiologie, Universiteit Gent, K L Ledeganckstraat 35, B 9000 Gent, Belgium
    FEMS Microbiol Lett 242:117-26. 2005

Detail Information

Publications104 found, 100 shown here

  1. ncbi Classification of Centers for Disease Control Group Eugonic Fermenter (EF)-4a and EF-4b as Neisseria animaloris sp. nov. and Neisseria zoodegmatis sp. nov., respectively
    Peter Vandamme
    Laboratorium voor Microbiologie, Universiteit Gent, Gent, Belgium
    Int J Syst Evol Microbiol 56:1801-5. 2006
    ..nov. (type strain LMG 23011(T)=NCTC 12228(T)) and Neisseria zoodegmatis sp. nov. (type strain LMG 23012(T)=NCTC 12230(T)), respectively...
  2. ncbi Cinnamaldehyde and cinnamaldehyde derivatives reduce virulence in Vibrio spp. by decreasing the DNA-binding activity of the quorum sensing response regulator LuxR
    Gilles Brackman
    Laboratory of Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B 9000 Ghent, Belgium
    BMC Microbiol 8:149. 2008
    ..The compounds were also evaluated in an in vivo assay measuring the reduction of Vibrio harveyi virulence towards Artemia shrimp...
  3. ncbi Phytosphingosine-1-phosphate is a signaling molecule involved in miconazole resistance in sessile Candida albicans cells
    Davy Vandenbosch
    Laboratory of Pharmaceutical Microbiology, Ghent University, Ghent, Belgium
    Antimicrob Agents Chemother 56:2290-4. 2012
    ..albicans cells...
  4. ncbi Eradication of Propionibacterium acnes biofilms by plant extracts and putative identification of icariin, resveratrol and salidroside as active compounds
    Tom Coenye
    Laboratory of Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B 9000 Ghent, Belgium
    Phytomedicine 19:409-12. 2012
    ....
  5. ncbi Development and evaluation of different normalization strategies for gene expression studies in Candida albicans biofilms by real-time PCR
    Heleen Nailis
    Laboratory for Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B 9000, Ghent, Belgium
    BMC Mol Biol 7:25. 2006
    ..To validate our normalization strategies we determined differences in ALS1 and ALS3 expression levels between C. albicans biofilm cells and their planktonic counterparts...
  6. ncbi Real-time PCR expression profiling of genes encoding potential virulence factors in Candida albicans biofilms: identification of model-dependent and -independent gene expression
    Heleen Nailis
    Laboratory for Pharmaceutical Microbiology, Universiteit Gent, Harelbekestraat 72, B 9000, Ghent, Belgium
    BMC Microbiol 10:114. 2010
    ....
  7. ncbi Transcriptional response of Burkholderia cenocepacia J2315 sessile cells to treatments with high doses of hydrogen peroxide and sodium hypochlorite
    Elke Peeters
    Laboratory of Pharmaceutical Microbiology, Ghent University, Ghent, Belgium
    BMC Genomics 11:90. 2010
    ..cenocepacia J2315 cells following exposure to high levels of H2O2 or NaOCl...
  8. ncbi Extracting phylogenetic information from whole-genome sequencing projects: the lactic acid bacteria as a test case
    Tom Coenye
    Laboratorium voor Microbiologie, Universiteit Gent, K L Ledeganckstraat 35, B 9000 Gent, Belgium
    Microbiology 149:3507-17. 2003
    ..Although these conclusions are based on a relatively small data set, they are largely in agreement with other studies and it is anticipated that similar trends will be observed when comparing other genomes...
  9. ncbi Advenella incenata gen. nov., sp. nov., a novel member of the Alcaligenaceae, isolated from various clinical samples
    Tom Coenye
    Laboratorium voor Microbiologie, Universiteit Gent, Gent, Belgium
    Int J Syst Evol Microbiol 55:251-6. 2005
    ..nov. (type strain LMG 22250T=CCUG 45225T) and five currently unnamed genomic species. The DNA G+C content of members of the novel genus Advenella is between 54.0 and 57.7 mol%...
  10. ncbi Organisation of the S10, spc and alpha ribosomal protein gene clusters in prokaryotic genomes
    Tom Coenye
    Laboratorium voor Microbiologie, Universiteit Gent, K L Ledeganckstraat 35, B 9000 Gent, Belgium
    FEMS Microbiol Lett 242:117-26. 2005
    ....
  11. ncbi Burkholderia phenoliruptrix sp. nov., to accommodate the 2,4,5-trichlorophenoxyacetic acid and halophenol-degrading strain AC1100
    Tom Coenye
    Laboratorium voor Microbiologie, Universiteit Gent, Gent, Belgium
    Syst Appl Microbiol 27:623-7. 2004
    ..Our data show that strain AC1100 represents a novel species for which the name Burkholderia phenoliruptrix sp. nov. is proposed. The type strain is AC1100T (= LMG 22037T = CCUG 48558T)...
  12. ncbi Recovery of Burkholderia cenocepacia strain PHDC from cystic fibrosis patients in Europe
    T Coenye
    Laboratory of Microbiology, Ghent University, Ghent, Belgium
    Thorax 59:952-4. 2004
    ....
  13. ncbi Stenotrophomonas africana Drancourt et al. 1997 is a later synonym of Stenotrophomonas maltophilia (Hugh 1981) Palleroni and Bradbury 1993
    Tom Coenye
    Laboratorium voor Microbiologie, Universiteit Gent, Gent, Belgium
    Int J Syst Evol Microbiol 54:1235-7. 2004
    ..A DNA-DNA binding level of 70% between both type strains confirmed that S. africana and S. maltophilia represent the same taxon. It is concluded that S. africana is a later synonym of S. maltophilia...
  14. ncbi A genomic perspective on the relationship between the Aquificales and the epsilon-Proteobacteria
    Tom Coenye
    Laboratorium voor Microbiologie, Universiteit Gent, Gent, Belgium
    Syst Appl Microbiol 27:313-22. 2004
    ..In addition, there is considerable evidence that confirms the placement of the Aquificales near the root of the bacterial tree...
  15. ncbi Intragenomic heterogeneity between multiple 16S ribosomal RNA operons in sequenced bacterial genomes
    Tom Coenye
    Laboratorium voor Microbiologie, Ghent University, K L Ledeganckstraat 35, B 9000 Ghent, Belgium
    FEMS Microbiol Lett 228:45-9. 2003
    ..Most heterogeneity occurred in variable regions V1, V2, and V6...
  16. ncbi Use of the genomic signature in bacterial classification and identification
    Tom Coenye
    Laboratorium voor Microbiologie, Ghent University, Gent, Belgium
    Syst Appl Microbiol 27:175-85. 2004
    ..Using the genomic signature we have also predicted DNA-DNA binding values for fastidious or unculturable endosymbionts belonging to the genera Rickettsia, Wigglesworthia and Buchnera...
  17. ncbi Identification of genomic groups in the genus Stenotrophomonas using gyrB RFLP analysis
    Tom Coenye
    Laboratorium voor Microbiologie, Universiteit Gent, KL Ledeganckstraat 35, B 9000 Gent, Belgium
    FEMS Immunol Med Microbiol 40:181-5. 2004
    ..Our data also indicate that the majority of CF isolates grouped in two clusters. This may indicate that isolates from specific genomic groups have an increased potential for colonisation of the respiratory tract of CF patients...
  18. ncbi Towards a prokaryotic genomic taxonomy
    Tom Coenye
    Laboratory of Microbiology, Ghent University, Ledeganckstraat 35, B 9000 Ghent, Belgium
    FEMS Microbiol Rev 29:147-67. 2005
    ....
  19. ncbi Displacement of epsilon-proteobacterial core genes by horizontally transferred homologous genes
    Tom Coenye
    Laboratorium voor Microbiologie, Universiteit Ghent, Ghent, Belgium
    Res Microbiol 156:738-47. 2005
    ..We did not observe HGT from Archaea to the epsilon-Proteobacteria. The majority of acquired genes were operational genes involved in transport, metabolism, signal transduction and energy production and conversion...
  20. ncbi Overrepresentation of immunostimulatory CpG motifs in Burkholderia genomes
    Tom Coenye
    Laboratorium voor Farmaceutische, Microbiologie, Universiteit Gent, Belgium
    J Cyst Fibros 4:193-6. 2005
    ..This suggests that DNA from Burkholderia sp. has the potential to cause significant inflammatory response. Whether this contributes significantly to the airway inflammation often observed in infected CF patients remains to be determined...
  21. ncbi Molecular mechanisms of chlorhexidine tolerance in Burkholderia cenocepacia biofilms
    Tom Coenye
    Laboratory of Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, 9000 Ghent, Belgium
    Antimicrob Agents Chemother 55:1912-9. 2011
    ..We also observed the differential expression of 19 genes carrying putative small RNA molecules, indicating a novel role for these regulatory elements in chlorhexidine tolerance...
  22. ncbi In vitro and in vivo model systems to study microbial biofilm formation
    Tom Coenye
    Laboratory of Pharmaceutical Microbiology, Ghent University, Gent, Belgium
    J Microbiol Methods 83:89-105. 2010
    ..In this review a comprehensive overview of various in vitro and in vivo systems is presented, and their advantages and disadvantages are discussed...
  23. ncbi Social interactions in the Burkholderia cepacia complex: biofilms and quorum sensing
    Tom Coenye
    Laboratory of Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B 9000 Gent, Belgium
    Future Microbiol 5:1087-99. 2010
    ..cepacia complex biofilm formation, the increased resistance of sessile B. cepacia complex cells and the role of quorum sensing in B. cepacia complex biofilm formation...
  24. ncbi Response of sessile cells to stress: from changes in gene expression to phenotypic adaptation
    Tom Coenye
    Laboratory of Pharmaceutical Microbiology, Ghent University, Gent, Belgium
    FEMS Immunol Med Microbiol 59:239-52. 2010
    ....
  25. ncbi Factors influencing the trailing endpoint observed in Candida albicans susceptibility testing using the CLSI procedure
    T Coenye
    Laboratorium voor Farmaceutische Microbiologie, Universiteit Gent, Gent, Belgium
    Clin Microbiol Infect 14:495-7. 2008
    ..In order to reduce the incidence of trailing growth according to the CLSI procedure, the use of higher glucose concentrations and flat-bottomed microtitre plates could be valuable improvements...
  26. ncbi Use of the modified Robbins device to study the in vitro biofilm removal efficacy of NitrAdine, a novel disinfecting formula for the maintenance of oral medical devices
    T Coenye
    Laboratorium voor Farmaceutische Microbiologie, Universiteit Gent, Gent, Belgium
    J Appl Microbiol 105:733-40. 2008
    ....
  27. ncbi Identification of putative noncoding RNA genes in the Burkholderia cenocepacia J2315 genome
    Tom Coenye
    Laboratorium voor Microbiologie, Universiteit Gent, Gent, Belgium
    FEMS Microbiol Lett 276:83-92. 2007
    ..The presence of a large number of ncRNA genes in this organism may help to explain its complexity, phenotypic variability and ability to survive in a remarkably wide range of environments...
  28. ncbi Biofilm formation by Propionibacterium acnes is associated with increased resistance to antimicrobial agents and increased production of putative virulence factors
    Tom Coenye
    Laboratorium voor Farmaceutische Microbiologie, Universiteit Gent, Harelbekestraat 72, B 9000 Gent, Belgium
    Res Microbiol 158:386-92. 2007
    ..In addition, sessile cells produce more extracellular lipases as well as significant amounts of the quorum-sensing molecule autoinducer-2...
  29. ncbi In vitro inhibition of Streptococcus mutans biofilm formation on hydroxyapatite by subinhibitory concentrations of anthraquinones
    Tom Coenye
    Laboratorium voor Farmaceutische Microbiologie, Universiteit Gent, Harelbekestraat 72, B 9000 Gent, Belgium
    Antimicrob Agents Chemother 51:1541-4. 2007
    ..Although AQs are known to generate reactive oxygen species, the latter do not underlie the observed effect. Our results suggest that AQs inhibit S. mutans biofilm formation by causing membrane perturbation...
  30. ncbi Characterization of mononucleotide repeats in sequenced prokaryotic genomes
    Tom Coenye
    Laboratorium voor Microbiologie, Universiteit Gent K L, Gent, Belgium
    DNA Res 12:221-33. 2005
    ..Finally, we also noted that genomes that contain a high fraction of horizontally transferred genes have a lower mononucleotide SSR density and that A and T are generally overrepresented in mononucleotide SSRs...
  31. ncbi A golden age for microbial genomics
    Tom Coenye
    Laboratorium voor Microbiologie, Ghent University, K, L, Ledeganckstraat 35, B-9000 Ghent, Belgium
    Genome Biol 4:347. 2003
  32. ncbi Ralstonia respiraculi sp. nov., isolated from the respiratory tract of cystic fibrosis patients
    Tom Coenye
    Department of Pediatrics, University of Michigan, 1150 W Med Ctr Dr, Ann Arbor, MI 48109 0646, USA
    Int J Syst Evol Microbiol 53:1339-42. 2003
    ..nov. is proposed; the other isolate was phylogenetically closely related to R. respiraculi, but is likely to represent another novel Ralstonia species. The type strain of R. respiraculi is AU3313(T) (=LMG 21510(T)=CCUG 46809(T))...
  33. ncbi Diversity and significance of Burkholderia species occupying diverse ecological niches
    Tom Coenye
    Laboratory of Microbiology, Ghent University, K L Ledeganckstraat 35, B 9000 Gent, Belgium
    Environ Microbiol 5:719-29. 2003
    ....
  34. ncbi Biofilms in skin infections: Propionibacterium acnes and acne vulgaris
    T Coenye
    Laboratory for Pharmaceutical Microbiology, Ghent University, Ghent, Belgium, Oystershell NV, Drongen, Belgium
    Infect Disord Drug Targets 8:156-9. 2008
    ..In addition, in vitro and in vivo biofilm formation has also been demonstrated for other microorganisms involved in skin diseases (including Staphylococcus aureus and Streptococcus pyogenes)...
  35. ncbi Classification of Ralstonia pickettii-like isolates from the environment and clinical samples as Ralstonia insidiosa sp. nov
    Tom Coenye
    Department of Pediatrics and Communicable Diseases, University of Michigan, 1150 W Med Ctr Dr, MSRB III, Rm 8323, Ann Arbor, MI 48109 0646, USA
    Int J Syst Evol Microbiol 53:1075-80. 2003
    ..Some outstanding issues in the taxonomy of the genus Ralstonia are also discussed...
  36. ncbi Description of Pandoraea gen. nov. with Pandoraea apista sp. nov., Pandoraea pulmonicola sp. nov., Pandoraea pnomenusa sp. nov., Pandoraea sputorum sp. nov. and Pandoraea norimbergensis comb. nov
    T Coenye
    Laboratorium voor Microbiologie, Universiteit Gent, Belgium
    Int J Syst Evol Microbiol 50:887-99. 2000
    ..1997) comb. nov. (type strain is LMG 18379T). The available clinical data indicate that at least some of these organisms may cause chronic infection in, and can be transmitted amongst, cystic fibrosis patients...
  37. ncbi Assignment of Centers for Disease Control group IVc-2 to the genus Ralstonia as Ralstonia paucula sp. nov
    P Vandamme
    Laboratorium voor Microbiologie, Gent, Belgium
    Int J Syst Bacteriol 49:663-9. 1999
    ..Strains have been isolated from various human clinical and environmental sources...
  38. ncbi Burkholderia cepacia genomovar VI, a new member of the Burkholderia cepacia complex isolated from cystic fibrosis patients
    T Coenye
    Laboratorium voor Microbiologie, Universiteit Gent, Belgium
    Int J Syst Evol Microbiol 51:271-9. 2001
    ..Retrospective analysis of epidemiological and genotypic data suggests that strains of B. cepacia genomovar VI have been involved in chronic colonization of CF patients and have been spread from person to person...
  39. ncbi Classification of Alcaligenes faecalis-like isolates from the environment and human clinical samples as Ralstonia gilardii sp. nov
    T Coenye
    Laboratorium voor Microbiologie, Universiteit Gent, Belgium
    Int J Syst Bacteriol 49:405-13. 1999
    ..The six environmental and four human isolates belonged to the genus Ralstonia and were assigned to a new species for which the name Ralstonia gilardii sp. nov. is proposed. The type strain is LMG 5886T...
  40. ncbi Burkholderia ambifaria sp. nov., a novel member of the Burkholderia cepacia complex including biocontrol and cystic fibrosis-related isolates
    T Coenye
    Laboratorium voor Microbiologie, Universiteit Gent, Ghent, Belgium
    Int J Syst Evol Microbiol 51:1481-90. 2001
    ..cepacia complex would be ill-advised until more is known about their potential pathogenic mechanisms...
  41. ncbi Polyphasic characterisation of Burkholderia cepacia-like isolates leading to the emended description of Burkholderia pyrrocinia
    Virginie Storms
    Laboratory for Microbiology, Faculty of Sciences, University of Ghent, Ghent, Belgium
    Syst Appl Microbiol 27:517-26. 2004
    ..cepacia complex species thus far comprising one single soil isolate only. An emended description of Burkholderia pyrrocinia is proposed. The taxonomic status of the remaining two isolates requires further analysis...
  42. ncbi Occurrence of multiple genomovars of Burkholderia cepacia in cystic fibrosis patients and proposal of Burkholderia multivorans sp. nov
    P Vandamme
    Laboratory of Microbiology, Universiteit Gent, Belgium
    Int J Syst Bacteriol 47:1188-200. 1997
    ..The data presented also demonstrate that Pseudomonas glathei and Pseudomonas pyrrocinia should be reclassified as Burkholderia species...
  43. ncbi High-resolution genotyping of Aspergillus fumigatus isolates recovered from chronically colonised patients with cystic fibrosis
    L M E Vanhee
    Laboratory for Pharmaceutical Microbiology, Ghent University, Ghent, Belgium
    Eur J Clin Microbiol Infect Dis 27:1005-7. 2008
    ..Additionally, some genotypes were isolated recurrently, indicating that they are capable of prolonged colonisation...
  44. ncbi Burkholderia fungorum sp. nov. and Burkholderia caledonica sp. nov., two new species isolated from the environment, animals and human clinical samples
    T Coenye
    Laboratorium voor Microbiologie, Universiteit Gent, Belgium
    Int J Syst Evol Microbiol 51:1099-107. 2001
    ..phenazinium, B. caledonica and B. fungorum include the following: assimilation of trehalose, citrate, DL-norleucine, adipate and sucrose; nitrate reduction; growth in the presence of 0.5% NaCl; and beta-galactosidase activity...
  45. ncbi Identification and population structure of Burkholderia stabilis sp. nov. (formerly Burkholderia cepacia genomovar IV)
    P Vandamme
    Laboratorium voor Microbiologie, Faculteit Wetenschappen, Universiteit Gent, Ghent, Belgium
    J Clin Microbiol 38:1042-7. 2000
    ..cepacia strains...
  46. ncbi Identification of Burkholderia species and genomovars from cystic fibrosis patients by AFLP fingerprinting
    T Coenye
    Laboratorium voor Microbiologie, Universiteit Gent, Belgium
    Int J Syst Bacteriol 49:1657-66. 1999
    ....
  47. ncbi Classification of metal-resistant bacteria from industrial biotopes as Ralstonia campinensis sp. nov., Ralstonia metallidurans sp. nov. and Ralstonia basilensis Steinle et al. 1998 emend
    J Goris
    Laboratorium voor Microbiologie, Universiteit Gent, Belgium
    Int J Syst Evol Microbiol 51:1773-82. 2001
    ..Six isolates were allocated to Ralstonia basilensis, which presently contains only the type strain; an emendation of the latter species description is therefore proposed...
  48. ncbi Burkholderia anthina sp. nov. and Burkholderia pyrrocinia, two additional Burkholderia cepacia complex bacteria, may confound results of new molecular diagnostic tools
    Peter Vandamme
    Laboratorium voor Microbiologie, Universiteit Gent, Ghent, Belgium
    FEMS Immunol Med Microbiol 33:143-9. 2002
    ..cepacia complex. This highlights another potential source for diagnostic problems with B. cepacia-like bacteria...
  49. ncbi Pseudomonas antimicrobica Attafuah and Bradbury 1990 is a junior synonym of Burkholderia gladioli (Severini 1913) Yabuuchi et al. 1993
    T Coenye
    Laboratory of Microbiology, Universiteit Gent, Ghent, Belgium
    Int J Syst Evol Microbiol 50:2135-9. 2000
    ..As Burkholderia gladioli is known to cause infections in patients with cystic fibrosis and chronic granulomatous disease, the eventual use of strain LMG 18920T as a biological control agent should be approached with caution...
  50. ncbi Evaluation of Nd:YAG and Er:YAG irradiation, antibacterial photodynamic therapy and sodium hypochlorite treatment on Enterococcus faecalis biofilms
    M A Meire
    Department of Operative Dentistry and Endodontology, Dental School, Ghent University, Gent, Belgium
    Int Endod J 45:482-91. 2012
    ....
  51. ncbi Evaluation of the efficacy of disinfection procedures against Burkholderia cenocepacia biofilms
    E Peeters
    Laboratory for Pharmaceutical Microbiology, Ghent University, Ghent, Belgium
    J Hosp Infect 70:361-8. 2008
    ..In conclusion, our study indicates that testing of the efficacy of disinfectants should be performed on both planktonic and sessile cells, with particular attention to their effects on cellular viability...
  52. ncbi Genotypic and chemotaxonomic evidence for the reclassification of Pseudomonas woodsii (Smith 1911) Stevens 1925 as Burkholderia andropogonis (Smith 1911) Gillis et al. 1995
    T Coenye
    Laboratorium voor Microbiologie, Universiteit Gent, Ghent, Belgium
    Int J Syst Evol Microbiol 51:183-5. 2001
    ..andropogonis LMG 2129T (99.0%). The chemotaxonomic and genotypic data confirm that [P.] woodsii and B. andropogonis represent the same species, for which it is proposed to retain the name B. andropogonis...
  53. ncbi Burkholderia cocovenenans (van Damme et al. 1960) Gillis et al. 1995 and Burkholderia vandii Urakami et al. 1994 are junior synonyms of Burkholderia gladioli (Severini 1913) Yabuuchi et al. 1993 and Burkholderia plantarii (Azegami et al. 1987) Urakami et
    T Coenye
    Laboratory of Microbiology, Universiteit Gent, Belgium
    Int J Syst Bacteriol 49:37-42. 1999
    ..gladioli and B. cocovenenans, and (ii) B. plantarii and B. vandii each represent a single species. It is concluded that B. cocovenenans and B. vandii are junior synonyms of B. gladioli and B. plantarii, respectively...
  54. ncbi Identification of Pandoraea species by 16S ribosomal DNA-based PCR assays
    T Coenye
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan, USA
    J Clin Microbiol 39:4452-5. 2001
    ..The use of these PCR assays facilitates the identification of Pandoraea spp. and avoids the misidentification of a Pandoraea sp. as a B. cepacia complex isolate...
  55. ncbi Characterization of unusual bacteria isolated from respiratory secretions of cystic fibrosis patients and description of Inquilinus limosus gen. nov., sp. nov
    Tom Coenye
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    J Clin Microbiol 40:2062-9. 2002
    ....
  56. ncbi Use of the gyrB gene for the identification of Pandoraea species
    Tom Coenye
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, 8301 MSRB III, Box 0646, 1150 W Med Ctr Dr, Ann Arbor, MI 48109 0646, USA
    FEMS Microbiol Lett 208:15-9. 2002
    ..Our data also indicate that the taxonomic status of Pandoraea genomospecies 2 should be reinvestigated...
  57. ncbi AI-2 quorum-sensing inhibitors affect the starvation response and reduce virulence in several Vibrio species, most likely by interfering with LuxPQ
    Gilles Brackman
    Laboratory of Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B 9000 Ghent, Belgium
    Microbiology 155:4114-22. 2009
    ..anguillarum, and to protect gnotobiotic Artemia from V. harveyi-induced mortality...
  58. ncbi Population structure analysis of Burkholderia cepacia genomovar III: varying degrees of genetic recombination characterize major clonal complexes
    Tom Coenye
    Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, MI 48109, USA
    Microbiology 149:77-88. 2003
    ..The observation that genetic recombination is frequent within some B. cepacia genomovar III populations has important implications for the biotechnological use of B. cepacia complex species...
  59. ncbi Use of PCR analyses to define the distribution of Ralstonia species recovered from patients with cystic fibrosis
    Tom Coenye
    Laboratory for Microbiology, Faculty of Sciences, University of Ghent, Ghent, Belgium
    J Clin Microbiol 43:3463-6. 2005
    ..respiraculi and Ralstonia pickettii accounted for 19% and 18%, respectively. Ralstonia basilensis and Ralstonia metallidurans, species not previously recovered from human samples, were also identified...
  60. ncbi Infection by Ralstonia species in cystic fibrosis patients: identification of R. pickettii and R. mannitolilytica by polymerase chain reaction
    Tom Coenye
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, 48109 0646, USA
    Emerg Infect Dis 8:692-6. 2002
    ..To facilitate identification of R. mannitolilytica and R. pickettii, we developed 16S ribosomal DNA-based polymerase chain reaction assays that allow sensitive and specific identification of these species...
  61. ncbi Proposal to accommodate Burkholderia cepacia genomovar VI as Burkholderia dolosa sp. nov
    Karen Vermis
    Laboratory for Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B 9000 Ghent, Belgium
    Int J Syst Evol Microbiol 54:689-91. 2004
    ..cepacia-selective medium PCAT. Both 16S rDNA and recA RFLP analysis revealed unique B. dolosa restriction patterns. In addition, new 16S rDNA- and recA-based PCR assays allowed its specific identification...
  62. ncbi Evaluation of species-specific recA-based PCR tests for genomovar level identification within the Burkholderia cepacia complex
    Karen Vermis
    Laboratory of Pharmaceutical Microbiology, Ghent University, Belgium
    J Med Microbiol 51:937-40. 2002
    ..cepacia-like isolate and by the B. ubonensis type strain. The latter organism is a recently described soil bacterium. Its relationship to the various B. cepacia complex genomovars needs further study...
  63. ncbi PCR-based assay for differentiation of Pseudomonas aeruginosa from other Pseudomonas species recovered from cystic fibrosis patients
    Theodore Spilker
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan, 48109. Laboratorium voor Microbiologie, Universiteit Gent, Ghent, Belgium
    J Clin Microbiol 42:2074-9. 2004
    ..Thus, we have designed two PCR assays: one is specific for the genus Pseudomonas, while the other is specific for P. aeruginosa. Both assays show 100% sensitivity and specificity...
  64. ncbi Multilocus restriction typing: a novel tool for studying global epidemiology of Burkholderia cepacia complex infection in cystic fibrosis
    Tom Coenye
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, MI 48109 0646, USA
    J Infect Dis 185:1454-62. 2002
    ..The portability of MLRT data will facilitate comparison of data obtained in different laboratories. In addition, data obtained with MLRT can be used in studies of bacterial population structure...
  65. ncbi Burkholderia cenocepacia sp. nov.--a new twist to an old story
    Peter Vandamme
    Laboratory of Microbiology, University of Ghent, Ghent, Belgium
    Res Microbiol 154:91-6. 2003
    ..cepacia (genomovar I). A formal classification of B. cepacia genomovar III encompassing the recA lineages IIIA and IIIB, and the new recA lineages IIIC and IIID, as B. cenocepacia sp. nov., with LMG 16656 as the type strain, is proposed...
  66. ncbi Comparison of multiple typing methods for Aspergillus fumigatus
    L M E Vanhee
    Laboratory of Pharmaceutical Microbiology, Ghent University, Harelbekestraat, Ghent, Belgium
    Clin Microbiol Infect 15:643-50. 2009
    ..Therefore, this study suggests the use of STR typing for research concerning the local epidemiology of A. fumigatus, which requires a high discriminatory power...
  67. ncbi Ribosomal DNA-directed PCR for identification of Achromobacter (Alcaligenes) xylosoxidans recovered from sputum samples from cystic fibrosis patients
    Lixia Liu
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    J Clin Microbiol 40:1210-3. 2002
    ..The availability of this assay will enhance identification of A. xylosoxidans, thereby facilitating study of the pathogenic role of this species and improving infection control efforts in CF...
  68. ncbi Comparative assessment of genotyping methods for epidemiologic study of Burkholderia cepacia genomovar III
    Tom Coenye
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan 48109-0646, USA
    J Clin Microbiol 40:3300-7. 2002
    ..e., local epidemiology), whereas BOX-PCR fingerprinting is more appropriate for large-scale studies (i.e., global epidemiology). In this regard, BOX-PCR fingerprinting can be considered a rapid and easy alternative to MLRT...
  69. ncbi Identification by subtractive hybridization of a novel insertion element specific for two widespread Burkholderia cepacia genomovar III strains
    Lixia Liu
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan 48109-0646, USA
    J Clin Microbiol 41:2471-6. 2003
    ....
  70. ncbi Transcriptional response to fluconazole and amphotericin B in Candida albicans biofilms
    Heleen Nailis
    Laboratory of Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B 9000, Ghent, Belgium
    Res Microbiol 161:284-92. 2010
    ..albicans cells that might contribute to a resistant biofilm phenotype...
  71. ncbi Pilot study using doxycycline-releasing stents to ameliorate postoperative healing quality after sinus surgery
    Wouter Huvenne
    Upper Airway Research Laboratory URL, ENT Department, Ghent University Hospital, Ghent, Belgium
    Wound Repair Regen 16:757-67. 2008
    ....
  72. ncbi Use of quorum sensing inhibitors to interfere with biofilm formation and development in Burkholderia multivorans and Burkholderia cenocepacia
    Gilles Brackman
    Laboratory of Pharmaceutical Microbiology, Ghent University, Ghent, Belgium
    Res Microbiol 160:144-51. 2009
    ..When used at subinhibitory concentrations, several compounds interfered with biofilm formation by Burkholderia spp. Our results suggest that the QS inhibitors affect later stages of biofilm formation and detachment...
  73. ncbi Detection and quantification of viable airborne bacteria and fungi using solid-phase cytometry
    Lies M E Vanhee
    Laboratory of Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B 9000, Ghent, Belgium
    Nat Protoc 4:224-31. 2009
    ..The whole procedure takes 5 h to complete and results in the accurate quantification of airborne bacteria and fungi for samples with a low or high microbial load...
  74. ncbi In vitro activity of ceftazidime, ciprofloxacin, meropenem, minocycline, tobramycin and trimethoprim/sulfamethoxazole against planktonic and sessile Burkholderia cepacia complex bacteria
    Elke Peeters
    Laboratory of Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium
    J Antimicrob Chemother 64:801-9. 2009
    ..The goal of the present study was to obtain a comprehensive overview of the bacteriostatic and bactericidal effects of six commonly used antibiotics on planktonic as well as on sessile Burkholderia cepacia complex cells...
  75. ncbi Taxonomy of the genus Cupriavidus: a tale of lost and found
    Peter Vandamme
    Laboratorium voor Microbiologie, Faculteit Wetenschappen, Universiteit Gent, K L Ledeganckstraat 35, B 9000 Gent, Belgium
    Int J Syst Evol Microbiol 54:2285-9. 2004
    ..nov. (type strain LMG 3244(T)=CCUG 12507(T)), Cupriavidus respiraculi comb. nov. (type strain LMG 21510(T)=CCUG 46809(T)) and Cupriavidus taiwanensis comb. nov. (type strain LMG 19424(T)=CCUG 44338(T))...
  76. ncbi Arcobacter cibarius sp. nov., isolated from broiler carcasses
    Kurt Houf
    Department of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium
    Int J Syst Evol Microbiol 55:713-7. 2005
    ..These data demonstrate that the 20 isolates represent a single novel Arcobacter species, for which the name Arcobacter cibarius sp. nov. is proposed, with LMG 21996(T) (=CCUG 48482(T)) as the type strain...
  77. ncbi Arcobacter thereius sp. nov., isolated from pigs and ducks
    Kurt Houf
    Department of Veterinary Public Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium
    Int J Syst Evol Microbiol 59:2599-604. 2009
    ..nov. is proposed. The type strain is LMG 24486(T) (=CCUG 56902(T))...
  78. ncbi A novel strategy for the isolation and identification of environmental Burkholderia cepacia complex bacteria
    Elke Vanlaere
    Laboratory of Microbiology, Ghent University, Ledeganckstraat 35, B 9000 Ghent, Belgium
    FEMS Microbiol Lett 249:303-7. 2005
    ..Our results suggest that these organisms may be more widespread in the home environment than previously assumed and that plant associated soil and pond water may be reservoirs of B. cepacia complex infection in CF patients...
  79. ncbi Simple sequence repeats and compositional bias in the bipartite Ralstonia solanacearum GMI1000 genome
    Tom Coenye
    Laboratorium voor Microbiologie, Ghent University, K, L, Ledeganckstraat 35, B 9000 Gent, Belgium
    BMC Genomics 4:10. 2003
    ....
  80. ncbi Comparison of multiple methods for quantification of microbial biofilms grown in microtiter plates
    Elke Peeters
    Laboratory for Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, Ghent, Belgium
    J Microbiol Methods 72:157-65. 2008
    ..Nevertheless, our data show that some assays are less suitable for the quantification of biofilms of particular isolates (e.g. the CV assay for P. aeruginosa)...
  81. ncbi Opinion: Re-evaluating prokaryotic species
    Dirk Gevers
    Laboratory of Microbiology and the Bioinformatics and Evolutionary Genomics Research Group, Ghent University VIB, Ghent, Belgium
    Nat Rev Microbiol 3:733-9. 2005
    ..We also consider the potential, and difficulties, of assigning species status to biologically or ecologically meaningful sequence clusters...
  82. ncbi Quality control of fifteen probiotic products containing Saccharomyces boulardii
    L M E Vanhee
    Laboratory of Pharmaceutical Microbiology, Ghent University, Ghent, Belgium
    J Appl Microbiol 109:1745-52. 2010
    ..Significance and Impact of the Study:? To our knowledge, this is the first time SPC is used for the quality control of probiotics with S. boulardii. Additionally, we demonstrated the need for gastric protection and accurate storage...
  83. ncbi Inhibition of Candida albicans biofilm formation by antimycotics released from modified polydimethyl siloxane
    Kristof De Prijck
    Laboratory of Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, Ghent, 9000, Belgium
    Mycopathologia 169:167-74. 2010
    ..It is hypothesized that the reduction in biofilm formation by these compounds in a flow system occurs through a contact-dependent effect...
  84. ncbi Fungicidal activity of miconazole against Candida spp. biofilms
    Davy Vandenbosch
    Laboratory of Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B 9000 Ghent, Belgium
    J Antimicrob Chemother 65:694-700. 2010
    ..albicans cells. In the present study, the fungicidal activity of miconazole against in vitro-grown mature Candida biofilms, and its relationship with the induction of ROS and ROS-dependent apoptosis were examined...
  85. ncbi Bacterial whole-genome sequences: minimal information and strain availability
    Tom Coenye
    Laboratorium voor Microbiologie, Universiteit Gent, B-9000 Ghent, Belgium
    Microbiology 150:2017-8. 2004
  86. ncbi Resistance of planktonic and biofilm-grown Burkholderia cepacia complex isolates to the transition metal gallium
    Elke Peeters
    Laboratorium voor Farmaceutische Microbiologie, Universiteit Gent, Gent, Belgium
    J Antimicrob Chemother 61:1062-5. 2008
    ..The aim of the present study was to investigate the in vitro activity of gallium against planktonic and biofilm-grown B. cepacia complex isolates...
  87. ncbi Candida albicans biofilm formation on peptide functionalized polydimethylsiloxane
    Kristof De Prijck
    Laboratory of Pharmaceutical Microbiology, Ghent University, Ghent, Belgium
    Biofouling 26:269-75. 2010
    ..albicans biofilms, with reductions of 93% and 91%, respectively. The results indicate that the reductions are peptide dependent...
  88. ncbi Monitoring ALS1 and ALS3 gene expression during in vitro Candida albicans biofilm formation under continuous flow conditions
    Heleen Nailis
    Laboratory for Pharmaceutical Microbiology, Ghent University, Ghent, Belgium
    Mycopathologia 167:9-17. 2009
    ..Together, our data indicate an important role for ALS3 in the early phases of biofilm formation in the CDC reactor, probably related to adhesion of filaments, while the role of ALS1 is less clear...
  89. ncbi Effectiveness of different laser systems to kill Enterococcus faecalis in aqueous suspension and in an infected tooth model
    M A Meire
    Department of Operative Dentistry and Endodontology, Dental School, Ghent University, Gent, Belgium
    Int Endod J 42:351-9. 2009
    ..To assess the antibacterial action of laser irradiation (Nd:YAG, KTP), photo activated disinfection (PAD) and 2.5% sodium hypochlorite (NaOCl) on Enterococcus faecalis, in an aqueous suspension and in an infected tooth model...
  90. ncbi Quorum sensing inhibitors increase the susceptibility of bacterial biofilms to antibiotics in vitro and in vivo
    Gilles Brackman
    Laboratory of Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B 9000 Ghent, Belgium
    Antimicrob Agents Chemother 55:2655-61. 2011
    ..Our data suggest that QSI may increase the success of antibiotic treatment by increasing the susceptibility of bacterial biofilms and/or by increasing host survival following infection...
  91. ncbi Rapid and direct quantification of viable Candida species in whole blood by use of immunomagnetic separation and solid-phase cytometry
    Lies M E Vanhee
    Laboratory of Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B 9000 Ghent, Belgium
    J Clin Microbiol 48:1126-31. 2010
    ..albicans and at least one other Candida species were found in five samples. Additionally, in two cases, high numbers of cells (175 to 480 cells/ml of blood) were associated with an endovascular source of infection...
  92. ncbi Updated version of the Burkholderia cepacia complex experimental strain panel
    Tom Coenye
    J Clin Microbiol 41:2797-8. 2003
  93. ncbi DNA-DNA hybridization values and their relationship to whole-genome sequence similarities
    Johan Goris
    Center for Microbial Ecology, Michigan State University, East Lansing, MI 48824, USA
    Int J Syst Evol Microbiol 57:81-91. 2007
    ..It is concluded that ANI can accurately replace DDH values for strains for which genome sequences are available...
  94. ncbi Chryseobacterium vrystaatense sp. nov., isolated from raw chicken in a chicken-processing plant
    Hanli de Beer
    School for Agriculture and Environmental Science, Central University of Technology Free State, P Bag X20539, 1 Pres Brandt Street, Bloemfontein 9300, South Africa
    Int J Syst Evol Microbiol 55:2149-53. 2005
    ..Levels of DNA-DNA hybridization between strains of C. vrystaatense and Chryseobacterium reference species were below 46%. Strain LMG 22846(T) (=CCUG 50970(T)) was chosen as the type strain and has a DNA G+C content of 37.1 mol%...
  95. ncbi Genome update: rRNAs in sequenced microbial genomes
    David W Ussery
    Center for Biological Sequence Analysis, Department of Biotechnology, Building 208, The Technical University of Denmark, Lyngby, DK-2800, Denmark
    Microbiology 150:1113-5. 2004
  96. ncbi Pannonibacter phragmitetus, described from a Hungarian soda lake in 2003, had been recognized several decades earlier from human blood cultures as Achromobacter groups B and E
    Barry Holmes
    National Collection of Type Cultures, Health Protection Agency Centre for Infections, Colindale, London, UK
    Int J Syst Evol Microbiol 56:2945-8. 2006
    ..We present here the characteristics of the organism to facilitate its recognition in human clinical specimens and hence to determine its clinical significance...
  97. ncbi Burkholderia mimosarum sp. nov., isolated from root nodules of Mimosa spp. from Taiwan and South America
    Wen Ming Chen
    Department of Seafood Science, National Kaohsiung Marine University, No 142, Hai Chuan Road, Nan Tzu, Kaohsiung City 811, Taiwan
    Int J Syst Evol Microbiol 56:1847-51. 2006
    ..nov. is proposed. The type strain, PAS44(T) (=LMG 23256(T) =BCRC 17516(T)), was isolated from Mimosa pigra nodules in Taiwan...
  98. ncbi Enterococcus hermanniensis sp. nov., from modified-atmosphere-packaged broiler meat and canine tonsils
    Joanna Koort
    Department of Food and Environmental Hygiene, University of Helsinki, Helsinki, Finland
    Int J Syst Evol Microbiol 54:1823-7. 2004
    ..pallens and Enterococcus raffinosus type strains. The name Enterococcus hermanniensis sp. nov. is proposed, with strain LMG 12317T (= CCUG 48100T) as the type strain...
  99. ncbi Chryseobacterium piscium sp. nov., isolated from fish of the South Atlantic Ocean off South Africa
    Hanli de Beer
    School for Agriculture and Environmental Science, Central University of Technology Free State, Bloemfontein, South Africa
    Int J Syst Evol Microbiol 56:1317-22. 2006
    ..balustinum and C. scophthalmum. The four isolates are formally classified as Chryseobacterium piscium sp. nov., with strain LMG 23089(T) (=CCUG 51923(T)) as the type strain. Its DNA G + C content is 33.6 mol%...
  100. ncbi Diversity of Weissella viridescens strains associated with "Morcilla de Burgos"
    Joanna Koort
    Department of Food and Environmental Hygiene, University of Helsinki, Finland
    Int J Food Microbiol 109:164-8. 2006
    ..The somewhat divergent results of the pheno- and HindIII ribotyping indicate that W. viridescens is far more heterogeneous species than previously reported...
  101. ncbi Characterization of Pseudoalteromonas citrea and P. nigrifaciens isolated from different ecological habitats based on REP-PCR genomic fingerprints
    Elena P Ivanova
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore 21202, USA
    Syst Appl Microbiol 25:275-83. 2002
    ..It is concluded that REP, ERIC and BOX-PCR are effective in generating strain specific patterns that can be used to elucidate geographic distribution, with these genomic patterns providing a valuable biogeographic criterion...