Wout Boerjan

Summary

Affiliation: Ghent University
Country: Belgium

Publications

  1. ncbi request reprint Lignin biosynthesis
    Wout Boerjan
    Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, K L Ledeganckstraat 35, B 9000 Gent, Belgium
    Annu Rev Plant Biol 54:519-46. 2003
  2. pmc Downregulation of cinnamoyl-coenzyme A reductase in poplar: multiple-level phenotyping reveals effects on cell wall polymer metabolism and structure
    Jean Charles Leplé
    Department of Plant Systems Biology, Flanders Institute for Biotechnology, 9052 Gent, Belgium
    Plant Cell 19:3669-91. 2007
  3. pmc A systems biology view of responses to lignin biosynthesis perturbations in Arabidopsis
    Ruben Vanholme
    Department of Plant Systems Biology, VIB, B 9052 Ghent, Belgium
    Plant Cell 24:3506-29. 2012
  4. pmc Mass spectrometry-based sequencing of lignin oligomers
    Kris Morreel
    Department of Plant Systems Biology, Flanders Institute for Biotechnology, B 9052 Ghent, Belgium
    Plant Physiol 153:1464-78. 2010
  5. doi request reprint Engineering traditional monolignols out of lignin by concomitant up-regulation of F5H1 and down-regulation of COMT in Arabidopsis
    Ruben Vanholme
    Department of Plant Systems Biology, VIB, 9052 Gent, Belgium
    Plant J 64:885-97. 2010
  6. pmc Phenolic profiling of caffeic acid O-methyltransferase-deficient poplar reveals novel benzodioxane oligolignols
    Kris Morreel
    Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, B 9052 Ghent, Belgium
    Plant Physiol 136:4023-36. 2004
  7. pmc Improved saccharification and ethanol yield from field-grown transgenic poplar deficient in cinnamoyl-CoA reductase
    Rebecca Van Acker
    Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium
    Proc Natl Acad Sci U S A 111:845-50. 2014
  8. doi request reprint Caffeoyl shikimate esterase (CSE) is an enzyme in the lignin biosynthetic pathway in Arabidopsis
    Ruben Vanholme
    Department of Plant Systems Biology, VIB Flanders Institute for Biotechnology, Technologiepark 927, B 9052 Ghent, Belgium
    Science 341:1103-6. 2013
  9. doi request reprint Breeding with rare defective alleles (BRDA): a natural Populus nigra HCT mutant with modified lignin as a case study
    Bartel Vanholme
    Department of Plant Systems Biology, Flanders Institute for Biotechnology, Technologiepark 927, 9052, Gent, Belgium
    New Phytol 198:765-76. 2013
  10. doi request reprint Mass spectrometry-based fragmentation as an identification tool in lignomics
    Kris Morreel
    Department of Plant Systems Biology, VIB, B 9052 Ghent, Belgium
    Anal Chem 82:8095-105. 2010

Collaborators

Detail Information

Publications39

  1. ncbi request reprint Lignin biosynthesis
    Wout Boerjan
    Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, K L Ledeganckstraat 35, B 9000 Gent, Belgium
    Annu Rev Plant Biol 54:519-46. 2003
    ..In some cases, the potential value for agriculture of transgenic plants with modified lignin structure has been demonstrated. This review presents a current picture of monolignol biosynthesis, polymerization, and lignin structure...
  2. pmc Downregulation of cinnamoyl-coenzyme A reductase in poplar: multiple-level phenotyping reveals effects on cell wall polymer metabolism and structure
    Jean Charles Leplé
    Department of Plant Systems Biology, Flanders Institute for Biotechnology, 9052 Gent, Belgium
    Plant Cell 19:3669-91. 2007
    ..Finally, chemical pulping of wood derived from 5-year-old, field-grown transgenic lines revealed improved pulping characteristics, but growth was affected in all transgenic lines tested...
  3. pmc A systems biology view of responses to lignin biosynthesis perturbations in Arabidopsis
    Ruben Vanholme
    Department of Plant Systems Biology, VIB, B 9052 Ghent, Belgium
    Plant Cell 24:3506-29. 2012
    ..Together, our data provide insight into lignin biosynthesis and the metabolic network it is embedded in and provide a systems view of the plant's response to pathway perturbations...
  4. pmc Mass spectrometry-based sequencing of lignin oligomers
    Kris Morreel
    Department of Plant Systems Biology, Flanders Institute for Biotechnology, B 9052 Ghent, Belgium
    Plant Physiol 153:1464-78. 2010
    ..Interestingly, based on molecular mass data of the unknown monomeric and dimeric substructures, at least 10 unknown monomeric units or interunit linkage types were observed, one of which was identified as an arylglycerol end unit...
  5. doi request reprint Engineering traditional monolignols out of lignin by concomitant up-regulation of F5H1 and down-regulation of COMT in Arabidopsis
    Ruben Vanholme
    Department of Plant Systems Biology, VIB, 9052 Gent, Belgium
    Plant J 64:885-97. 2010
    ..These genetic interactions must be taken into account in future cell-wall engineering strategies...
  6. pmc Phenolic profiling of caffeic acid O-methyltransferase-deficient poplar reveals novel benzodioxane oligolignols
    Kris Morreel
    Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, B 9052 Ghent, Belgium
    Plant Physiol 136:4023-36. 2004
    ....
  7. pmc Improved saccharification and ethanol yield from field-grown transgenic poplar deficient in cinnamoyl-CoA reductase
    Rebecca Van Acker
    Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium
    Proc Natl Acad Sci U S A 111:845-50. 2014
    ..We conclude that CCR down-regulation may become a successful strategy to improve biomass processing if the variability in down-regulation and the yield penalty can be overcome...
  8. doi request reprint Caffeoyl shikimate esterase (CSE) is an enzyme in the lignin biosynthetic pathway in Arabidopsis
    Ruben Vanholme
    Department of Plant Systems Biology, VIB Flanders Institute for Biotechnology, Technologiepark 927, B 9052 Ghent, Belgium
    Science 341:1103-6. 2013
    ..Collectively, these data necessitate the revision of currently accepted models of the lignin biosynthetic pathway. ..
  9. doi request reprint Breeding with rare defective alleles (BRDA): a natural Populus nigra HCT mutant with modified lignin as a case study
    Bartel Vanholme
    Department of Plant Systems Biology, Flanders Institute for Biotechnology, Technologiepark 927, 9052, Gent, Belgium
    New Phytol 198:765-76. 2013
    ..The proposed breeding strategy, called 'breeding with rare defective alleles' (BRDA), should be widely applicable, independent of the target gene or the species...
  10. doi request reprint Mass spectrometry-based fragmentation as an identification tool in lignomics
    Kris Morreel
    Department of Plant Systems Biology, VIB, B 9052 Ghent, Belgium
    Anal Chem 82:8095-105. 2010
    ..Consequently, this work lays the foundation for a detailed characterization of the lignome in different plant species, mutants, and transgenics and for the MS-based sequencing of lignin oligomers and (neo)lignans...
  11. ncbi request reprint Gene expression during the induction, maintenance, and release of dormancy in apical buds of poplar
    Antje Rohde
    Department of Plant Systems Biology, Flanders Institute for Biotechnology VIB, 9052 Gent, Belgium
    J Exp Bot 58:4047-60. 2007
    ..These regulatory genes could be involved in the differentiation of stipule-like leaf organs protecting the bud, or act during the growth-dormancy transition in the meristem, revealing commonalities between para- and endodormancy...
  12. ncbi request reprint Genetical metabolomics of flavonoid biosynthesis in Populus: a case study
    Kris Morreel
    Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology VIB, Ghent University, B 9052 Gent, Belgium
    Plant J 47:224-37. 2006
    ..The data indicate that the combination of metabolite profiling with QTL analysis is a valuable tool to identify control points in a complex metabolic pathway of closely related compounds...
  13. pmc Systematic structural characterization of metabolites in Arabidopsis via candidate substrate-product pair networks
    Kris Morreel
    Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium
    Plant Cell 26:929-45. 2014
    ..This method will greatly advance the value of metabolomics in systems biology. ..
  14. doi request reprint Metabolic engineering of novel lignin in biomass crops
    Ruben Vanholme
    Department of Plant Systems Biology, VIB, Technologiepark 927, Gent, Belgium
    New Phytol 196:978-1000. 2012
    ..Finally, the status of detection and identification of compounds by phenolic profiling is discussed, as phenolic profiling serves in pathway elucidation and for the detection of incorporation of alternative lignin monomers...
  15. doi request reprint Lignin engineering
    Ruben Vanholme
    Department of Plant Systems Biology, Flanders Institute for Biotechnology VIB, Technologiepark 927, Gent, Belgium
    Curr Opin Plant Biol 11:278-85. 2008
    ....
  16. pmc Mapping methyl jasmonate-mediated transcriptional reprogramming of metabolism and cell cycle progression in cultured Arabidopsis cells
    Laurens Pauwels
    Department of Plant Systems Biology, Flanders Institute for Biotechnology, Ghent University, 9052 Ghent, Belgium
    Proc Natl Acad Sci U S A 105:1380-5. 2008
    ....
  17. pmc A molecular timetable for apical bud formation and dormancy induction in poplar
    Tom Ruttink
    Department of Plant Systems Biology, Flanders Institute for Biotechnology, 9052 Gent, Belgium
    Plant Cell 19:2370-90. 2007
    ....
  18. doi request reprint Bud set in poplar--genetic dissection of a complex trait in natural and hybrid populations
    Antje Rohde
    Department of Plant Systems Biology, Flanders Institute for Biotechnology VIB, Gent, Belgium
    New Phytol 189:106-21. 2011
    ..These regions of 1.83-4.25 Mbp in size, containing between 202 and 394 genes, form the basis for further molecular-genetic dissection of bud set...
  19. ncbi request reprint Molecular phenotyping of lignin-modified tobacco reveals associated changes in cell-wall metabolism, primary metabolism, stress metabolism and photorespiration
    Rebecca Dauwe
    Department of Plant Systems Biology, Flanders Institute for Biotechnology, Technologiepark 927, 9052 Gent, Belgium
    Plant J 52:263-85. 2007
    ..Our data provide a molecular picture of a plant's response to altered monolignol biosynthesis...
  20. pmc Accumulation of N-acetylglucosamine oligomers in the plant cell wall affects plant architecture in a dose-dependent and conditional manner
    Bartel Vanholme
    Department of Plant Systems Biology, Flanders Institute for Biotechnology, 9052 Ghent, Belgium
    Plant Physiol 165:290-308. 2014
    ....
  21. pmc Transcription factor WRKY23 assists auxin distribution patterns during Arabidopsis root development through local control on flavonol biosynthesis
    Wim Grunewald
    Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium
    Proc Natl Acad Sci U S A 109:1554-9. 2012
    ..Our results suggest a model in which WRKY23 is part of a transcriptional feedback loop of auxin on its own transport through local regulation of flavonol biosynthesis...
  22. doi request reprint Temperature signals contribute to the timing of photoperiodic growth cessation and bud set in poplar
    Antje Rohde
    Department of Plant Systems Biology, VIB, 9052 Gent, Belgium
    Tree Physiol 31:472-82. 2011
    ..The ability of perennial plants to integrate additional environmental signals with photoperiod signaling may add to short-term acclimatization to the predicted longer growing seasons in future climates...
  23. pmc Lignin biosynthesis perturbations affect secondary cell wall composition and saccharification yield in Arabidopsis thaliana
    Rebecca Van Acker
    Department of Plant Systems Biology, VIB, Technologiepark 927, Gent, 9052, Belgium
    Biotechnol Biofuels 6:46. 2013
    ..Saccharification models were built to elucidate which cell wall parameters played a role in cell wall recalcitrance...
  24. doi request reprint Potential of Arabidopsis systems biology to advance the biofuel field
    Ruben Vanholme
    Department of Plant Systems Biology, Flanders Institute for Biotechnology VIB, 9052 Ghent, Belgium
    Trends Biotechnol 28:543-7. 2010
    ..We develop our ideas with a focus on the lignin biosynthetic pathway, because lignin is among the most important factors determining cell wall recalcitrance...
  25. pmc Perturbation of indole-3-butyric acid homeostasis by the UDP-glucosyltransferase UGT74E2 modulates Arabidopsis architecture and water stress tolerance
    Vanesa B Tognetti
    Department of Plant Systems Biology, VIB, Ghent University, 9052 Gent, Belgium
    Plant Cell 22:2660-79. 2010
    ....
  26. pmc Modeling lignin polymerization. I. Simulation model of dehydrogenation polymers
    Frederik R D van Parijs
    Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium
    Plant Physiol 153:1332-44. 2010
    ..These are cleaved in analytical methods used to study lignin composition, namely thioacidolysis and derivatization followed by reductive cleavage, under pulping conditions, and in some lignocellulosic biomass pretreatments...
  27. ncbi request reprint Joint GC-MS and LC-MS platforms for comprehensive plant metabolomics: repeatability and sample pre-treatment
    Ruben t'Kindt
    Laboratory of Medical Biochemistry and Clinical Analysis, Ghent University, Harelbekestraat 72, B 9000 Ghent, Belgium
    J Chromatogr B Analyt Technol Biomed Life Sci 877:3572-80. 2009
    ..Below room temperature (instead of heated) extraction does not significantly degrade GC-MS performance but one should be more cautious with respect to residual enzymatic activity in the plant extract...
  28. pmc Towards a carbon-negative sustainable bio-based economy
    Bartel Vanholme
    Department of Plant Systems Biology, Flanders Institute for Biotechnology Gent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University Gent, Belgium
    Front Plant Sci 4:174. 2013
    ..These three different multidisciplinary pillars interact through the value chain of the bio-based economy...
  29. ncbi request reprint Biotechnology and the domestication of forest trees
    Wout Boerjan
    Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology VIB, Ghent University, Technologiepark 927, B 9052 Gent, Belgium
    Curr Opin Biotechnol 16:159-66. 2005
    ..Not only has the potential of transgenic trees with optimized yield and quality traits been demonstrated in field trials, but progress in genetical genomics and association genetics promise quantum leaps forward for tree improvement...
  30. pmc Profiling of oligolignols reveals monolignol coupling conditions in lignifying poplar xylem
    Kris Morreel
    Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, B 9052 Ghent, Belgium
    Plant Physiol 136:3537-49. 2004
    ..Importantly, the structures of two oligomers, each containing a gamma-p-hydroxybenzoylated syringyl unit, strongly suggest that sinapyl p-hydroxybenzoate is an authentic precursor for lignin polymerization in poplar...
  31. pmc PtABI3 impinges on the growth and differentiation of embryonic leaves during bud set in poplar
    Antje Rohde
    Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, K L Ledeganckstraat 35, B 9000 Gent, Belgium
    Plant Cell 14:1885-901. 2002
    ..These experiments reveal that ABI3 plays a role in the cellular differentiation of vegetative tissues, in addition to its function in seeds...
  32. pmc Genome-wide characterization of the lignification toolbox in Arabidopsis
    Jeroen Raes
    Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, Technologiepark 927, B 9052 Gent, Belgium
    Plant Physiol 133:1051-71. 2003
    ..Together, these data describe the full complement of monolignol biosynthesis genes in Arabidopsis, provide a unified nomenclature, and serve as a basis for further functional studies...
  33. ncbi request reprint Lignin: genetic engineering and impact on pulping
    Marie Baucher
    Laboratoire de Biotechnologie Végétale, Universite Libre de Bruxelles, Brussels, Belgium
    Crit Rev Biochem Mol Biol 38:305-50. 2003
    ..Fine-tuned modification of lignin content, composition, or both is now achievable and could have important economic and environmental benefits...
  34. doi request reprint Identification of the structure and origin of a thioacidolysis marker compound for ferulic acid incorporation into angiosperm lignins (and an indicator for cinnamoyl CoA reductase deficiency)
    John Ralph
    US Dairy Forage Research Center, USDA Agricultural Research Service, Madison, WI 53706, USA
    Plant J 53:368-79. 2008
    ..Moreover, ferulic acid incorporation provides a new mechanism for producing branch points in the polymer. The findings sharply contradict those reported in a recent study on CCR-deficient Arabidopsis...
  35. ncbi request reprint Molecular changes associated with the setting up of secondary growth in aspen
    Damien van Raemdonck
    Laboratory of Plant Biotechnology, Universite Libre de Bruxelles, chaussée de Wavre 1850, B 1160 Brussels, Belgium
    J Exp Bot 56:2211-27. 2005
    ..These results suggest that these genes have a potential role in vascular tissue development and/or functioning...
  36. ncbi request reprint Metabolite profiling reveals a role for atypical cinnamyl alcohol dehydrogenase CAD1 in the synthesis of coniferyl alcohol in tobacco xylem
    Isabelle Damiani
    UMR UPS CNRS 5546 Surfaces Cellulaires et Signalisation chez les Végétaux, Pole de Biotechnologie Vegetale, 24 chemin de Borderouge, BP 42617, F 31326, Castanet, France
    Plant Mol Biol 59:753-69. 2005
    ..Together, our results support a significant contribution of CAD1 to the synthesis of coniferyl alcohol in planta, along with the previously characterized CAD2 enzymes...
  37. ncbi request reprint Genetically modified lignin below ground
    David W Hopkins
    Nat Biotechnol 25:168-9. 2007
  38. doi request reprint Genome-wide identification of NBS resistance genes in Populus trichocarpa
    Annegret Kohler
    Unité Mixte de Recherche INRA UHP 1136 Interactions Arbres Microorganismes, Institut National de la Recherche Agronomique, Centre de Recherches de Nancy, 54280 Champenoux, France
    Plant Mol Biol 66:619-36. 2008
    ..The transcripts of only 34 NBS-LRR genes were detected in rust-infected and non-infected leaves using a whole-genome oligoarray. None showed an altered expression two days post inoculation...
  39. ncbi request reprint Field and pulping performances of transgenic trees with altered lignification
    Gilles Pilate
    Unité Amélioration, Génétique et Physiologie Forestières, INRA Orleans, BP 20619, Ardon, 45166 Olivet, France
    Nat Biotechnol 20:607-12. 2002
    ..This work highlights the potential of engineering wood quality for more environmentally benign papermaking without interfering with tree growth or fitness...