Yves F Dufrêne

Summary

Country: Belgium

Publications

  1. doi request reprint Chemical force microscopy of stimuli-responsive adhesive copolymers
    Audrey Beaussart
    Universite Catholique de Louvain, Institute of Life Sciences, Croix du Sud 1, bte L7 04 01, B 1348 Louvain la Neuve, Belgium
    Nanoscale 6:565-71. 2014
  2. pmc Single-cell force spectroscopy of the medically important Staphylococcus epidermidis-Candida albicans interaction
    Audrey Beaussart
    Universite Catholique de Louvain, Institute of Life Sciences, Croix du Sud, 1, bte L7 04 01, B 1348 Louvain la Neuve, Belgium
    Nanoscale 5:10894-900. 2013
  3. doi request reprint Multiparametric imaging of biological systems by force-distance curve-based AFM
    Yves F Dufrêne
    Institute of Life Sciences, Universite Catholique de Louvain, Louvain la Neuve, Belgium
    Nat Methods 10:847-54. 2013
  4. doi request reprint Single-cell force spectroscopy of pili-mediated adhesion
    Ruby May A Sullan
    Universite Catholique de Louvain, Institute of Life Sciences, Croix du Sud, 1, bte L7 04 01, B 1348 Louvain la Neuve, Belgium
    Nanoscale 6:1134-43. 2014
  5. doi request reprint Force nanoscopy of cell mechanics and cell adhesion
    Yves F Dufrêne
    Universite Catholique de Louvain, Institute of Life Sciences, Croix du Sud, 1, bte L7 04 01, B 1348 Louvain la Neuve, Belgium
    Nanoscale 5:4094-104. 2013
  6. doi request reprint Atomic force microscopy and chemical force microscopy of microbial cells
    Yves F Dufrêne
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    Nat Protoc 3:1132-8. 2008
  7. doi request reprint Towards nanomicrobiology using atomic force microscopy
    Yves F Dufrêne
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    Nat Rev Microbiol 6:674-80. 2008
  8. doi request reprint Atomic force microscopy: a powerful molecular toolkit in nanoproteomics
    Yves F Dufrêne
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Louvain la Neuve, Belgium
    Proteomics 9:5400-5. 2009
  9. doi request reprint Atomic force microscopy of fungal cell walls: an update
    Yves F Dufrêne
    Institute of Condensed Matter and Nanosciences, Universite Catholique de Louvain, Louvain la Neuve, Belgium
    Yeast 27:465-71. 2010
  10. doi request reprint Force generation: ATP-powered proteasomes pull the rope
    Yves F Dufrêne
    Institute of Condensed Matter and Nanosciences, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    Curr Biol 21:R427-30. 2011

Collaborators

Detail Information

Publications80

  1. doi request reprint Chemical force microscopy of stimuli-responsive adhesive copolymers
    Audrey Beaussart
    Universite Catholique de Louvain, Institute of Life Sciences, Croix du Sud 1, bte L7 04 01, B 1348 Louvain la Neuve, Belgium
    Nanoscale 6:565-71. 2014
    ..These nanoscale experiments show how solvent-induced conformational changes of stimuli-responsive copolymers can be used to modulate surface adhesion. ..
  2. pmc Single-cell force spectroscopy of the medically important Staphylococcus epidermidis-Candida albicans interaction
    Audrey Beaussart
    Universite Catholique de Louvain, Institute of Life Sciences, Croix du Sud, 1, bte L7 04 01, B 1348 Louvain la Neuve, Belgium
    Nanoscale 5:10894-900. 2013
    ..In the future, we anticipate that this SCFS platform will be used in nanomedicine to decipher the molecular mechanisms of a wide variety of pathogen-pathogen interactions and may help in designing novel anti-adhesion agents...
  3. doi request reprint Multiparametric imaging of biological systems by force-distance curve-based AFM
    Yves F Dufrêne
    Institute of Life Sciences, Universite Catholique de Louvain, Louvain la Neuve, Belgium
    Nat Methods 10:847-54. 2013
    ..Here we discuss the principles and applications of advanced FD-based AFM tools for the quantitative multiparametric characterization of complex cellular and biomolecular systems under physiological conditions. ..
  4. doi request reprint Single-cell force spectroscopy of pili-mediated adhesion
    Ruby May A Sullan
    Universite Catholique de Louvain, Institute of Life Sciences, Croix du Sud, 1, bte L7 04 01, B 1348 Louvain la Neuve, Belgium
    Nanoscale 6:1134-43. 2014
    ....
  5. doi request reprint Force nanoscopy of cell mechanics and cell adhesion
    Yves F Dufrêne
    Universite Catholique de Louvain, Institute of Life Sciences, Croix du Sud, 1, bte L7 04 01, B 1348 Louvain la Neuve, Belgium
    Nanoscale 5:4094-104. 2013
    ....
  6. doi request reprint Atomic force microscopy and chemical force microscopy of microbial cells
    Yves F Dufrêne
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    Nat Protoc 3:1132-8. 2008
    ..We also discuss recording conditions and data interpretation, and provide recommendations for reliable experiments. For well-trained AFM users, the entire protocol can be completed in 2-3 d...
  7. doi request reprint Towards nanomicrobiology using atomic force microscopy
    Yves F Dufrêne
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    Nat Rev Microbiol 6:674-80. 2008
    ..In addition, recent developments in cantilever nanosensors have opened up new avenues for the label-free detection of microorganisms and bioanalytes...
  8. doi request reprint Atomic force microscopy: a powerful molecular toolkit in nanoproteomics
    Yves F Dufrêne
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Louvain la Neuve, Belgium
    Proteomics 9:5400-5. 2009
    ..specific molecules, can be used to answer pertinent questions related to surface-associated proteins, such as what is their spatial arrangement on the cell surface, and what are the forces driving their interaction with the environment?..
  9. doi request reprint Atomic force microscopy of fungal cell walls: an update
    Yves F Dufrêne
    Institute of Condensed Matter and Nanosciences, Universite Catholique de Louvain, Louvain la Neuve, Belgium
    Yeast 27:465-71. 2010
    ..Here I highlight the advances made by my team in this area during the past 3 years...
  10. doi request reprint Force generation: ATP-powered proteasomes pull the rope
    Yves F Dufrêne
    Institute of Condensed Matter and Nanosciences, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    Curr Biol 21:R427-30. 2011
    ..A new study provides fascinating insights into the sophisticated mechanism by which an ATP-fueled proteolytic machine generates mechanical forces to unfold and translocate multidomain substrates...
  11. doi request reprint Microbial nanoscopy: a closer look at microbial cell surfaces
    Vincent Dupres
    Institute of Condensed Matter and Nanosciences, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    Trends Microbiol 18:397-405. 2010
    ....
  12. doi request reprint High-resolution imaging of chemical and biological sites on living cells using peak force tapping atomic force microscopy
    David Alsteens
    Institute of Life Sciences and Institute of Condensed Matter and Nanosciences, Universite Catholique de Louvain, Croix du Sud, 1, bte L7 04 01, B 1348 Louvain la Neuve, Belgium
    Langmuir 28:16738-44. 2012
    ....
  13. doi request reprint Nanoscale imaging of microbial pathogens using atomic force microscopy
    David Alsteens
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    Wiley Interdiscip Rev Nanomed Nanobiotechnol 1:168-80. 2009
    ..These noninvasive nanoscale analyses provide new avenues in pathogenesis research, particularly for investigating the action mode of antimicrobial drugs, and for elucidating the molecular basis of pathogen-host interactions...
  14. pmc Single-molecule imaging and functional analysis of Als adhesins and mannans during Candida albicans morphogenesis
    Audrey Beaussart
    Institute of Life Sciences and Institute of Condensed Matter and Nanosciences, Universite Catholique de Louvain, Croix du Sud, 1, bte L7 04 01, B 1348 Louvain la Neuve, Belgium
    ACS Nano 6:10950-64. 2012
    ..The single-molecule experiments presented here offer promising prospects for understanding how microbial pathogens use cell surface molecules to modulate biofilm and immune interactions...
  15. doi request reprint Stretching polysaccharides on live cells using single molecule force spectroscopy
    Gregory Francius
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Louvain la Neuve, Belgium
    Nat Protoc 4:939-46. 2009
    ..Even though the presented protocol is for L. rhamnosus, it can be easily modified for other cell types. For users having expertise in the field, the entire protocol can be completed in about 5 d...
  16. doi request reprint Molecular mapping of lipoarabinomannans on mycobacteria
    Claire Verbelen
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    Langmuir 25:4324-7. 2009
    ..This single-molecule study provides novel insight into the architecture of mycobacterial cell walls and offers promising perspectives for understanding the action modes of antimycobacterial drugs...
  17. doi request reprint Single-molecule analysis of the major glycopolymers of pathogenic and non-pathogenic yeast cells
    Sofiane El-Kirat-Chatel
    Universite Catholique de Louvain, Institute of Life Sciences, Croix du Sud, 1, bte L7 04 01, B 1348 Louvain la Neuve, Belgium
    Nanoscale 5:4855-63. 2013
    ..In nanomedicine, we anticipate that this new form of AFM-based nanoglycobiology will contribute to the development of sugar-based drugs, immunotherapeutics, vaccines and diagnostics...
  18. ncbi request reprint Ethambutol-induced alterations in Mycobacterium bovis BCG imaged by atomic force microscopy
    Claire Verbelen
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Louvain la Neuve, Belgium
    FEMS Microbiol Lett 264:192-7. 2006
    ..These noninvasive ultrastructural investigations provide novel information on the macromolecular architecture of the mycobacterial envelope as well as into the destructuring effects of ethambutol...
  19. doi request reprint Detection, localization, and conformational analysis of single polysaccharide molecules on live bacteria
    Gregory Francius
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    ACS Nano 2:1921-9. 2008
    ..Applicable to a wide variety of cells, this single molecule method offers exciting prospects for analyzing the heterogeneity and diversity of macromolecules constituting cell membranes and cell walls...
  20. pmc Single-molecule force spectroscopy of mycobacterial adhesin-adhesin interactions
    Claire Verbelen
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    J Bacteriol 189:8801-6. 2007
    ..Together with the fact that HBHA mediates bacterial aggregation, these observations suggest that the single homophilic HBHA interactions measured here reflect the formation of multimers that may promote mycobacterial aggregation...
  21. doi request reprint The yeast Wsc1 cell surface sensor behaves like a nanospring in vivo
    Vincent Dupres
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Louvain la Neuve, Belgium
    Nat Chem Biol 5:857-62. 2009
    ....
  22. doi request reprint Adhesion and nanomechanics of pili from the probiotic Lactobacillus rhamnosus GG
    Prachi Tripathi
    Institute of Life Sciences, Universite Catholique de Louvain, Croix du Sud, 1, bte L7 04 01, B 1348 Louvain la Neuve, Belgium
    ACS Nano 7:3685-97. 2013
    ..The single-molecule experiments presented here may help us to design molecules capable of promoting or inhibiting bacterial-host interactions...
  23. ncbi request reprint Chemical force microscopy of single live cells
    Etienne Dague
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    Nano Lett 7:3026-30. 2007
    ....
  24. ncbi request reprint Organization of the mycobacterial cell wall: a nanoscale view
    David Alsteens
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, 1348 Louvain la Neuve, Belgium
    Pflugers Arch 456:117-25. 2008
    ..Taken together, these structural, chemical, and immunological data provide novel insight into the action mode of antimycobacterial drugs, as well as into the spatial organization of the mycobacterial cell wall...
  25. pmc Interaction of the mycobacterial heparin-binding hemagglutinin with actin, as evidenced by single-molecule force spectroscopy
    Claire Verbelen
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    J Bacteriol 190:7614-20. 2008
    ..Taken together, our data indicate that HBHA is able to specifically bind actin, via both its N-terminal and C-terminal domains, strongly suggesting a role of the HBHA-actin interaction in the pathogenesis of mycobacterial diseases...
  26. ncbi request reprint Direct measurement of hydrophobic forces on cell surfaces using AFM
    David Alsteens
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, Louvain la Neuve, Belgium
    Langmuir 23:11977-9. 2007
    ..Then, we measured hydrophobic forces associated with mycolic acids on the surface of mycobacteria, supporting the notion that these hydrophobic compounds represent an important permeation barrier to drugs...
  27. doi request reprint Force spectroscopy of the interaction between mycobacterial adhesins and heparan sulphate proteoglycan receptors
    Vincent Dupres
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    Chemphyschem 10:1672-5. 2009
    ..These single-molecule measurements provide new avenues in pathogenesis research, particularly for elucidating the molecular basis of pathogen-host interactions...
  28. doi request reprint In vivo imaging of S-layer nanoarrays on Corynebacterium glutamicum
    Vincent Dupres
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    Langmuir 25:9653-5. 2009
    ..These nanoscale analyses open new avenues for understanding the structure of protein monomolecular arrays, which is a crucial challenge in current nanoscience and life science research...
  29. doi request reprint Nanoscale membrane activity of surfactins: influence of geometry, charge and hydrophobicity
    Gregory Francius
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgique
    Biochim Biophys Acta 1778:2058-68. 2008
    ..Also, this study suggests that synthetic analogs are excellent candidates for developing new surfactants with tunable, well-defined properties for medical and biotechnological applications...
  30. doi request reprint AFM for nanoscale microbe analysis
    Yves F Dufrêne
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    Analyst 133:297-301. 2008
    ..With this Highlight on AFM, it is hoped that more and more microbiologists and biophysicists will take advantage of this powerful, multifunctional nanotechnique...
  31. doi request reprint Interactions of oritavancin, a new semi-synthetic lipoglycopeptide, with lipids extracted from Staphylococcus aureus
    Oscar Domenech
    Universite Catholique de Louvain, Louvain Drug Research Institute, Unité de Pharmacologie Cellulaire et Moléculaire, UCL 73 70, Avenue E Mounier 73, B 1200 Bruxelles, Belgium
    Biochim Biophys Acta 1798:1876-85. 2010
    ..This work shows how AFM and biophysical methods may help in characterizing drug-membrane interactions, and sheds further light on the mode of action of oritavancin...
  32. pmc Single-cell force spectroscopy of probiotic bacteria
    Audrey Beaussart
    Universite Catholique de Louvain, Institute of Life Sciences, Louvain la Neuve, Belgium
    Biophys J 104:1886-92. 2013
    ..plantarum, and represent a generic platform for studying the molecular mechanisms of cell adhesion in probiotic and pathogenic bacteria...
  33. doi request reprint Deciphering the nanometer-scale organization and assembly of Lactobacillus rhamnosus GG pili using atomic force microscopy
    Prachi Tripathi
    Institute of Condensed Matter and Nanosciences, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    Langmuir 28:2211-6. 2012
    ..Our data suggest that bacterial pili may provide a generic approach in nanobiotechnology for elaborating functional supramolecular interfaces assembled from bioactive building blocks...
  34. ncbi request reprint Towards a nanoscale view of fungal surfaces
    Etienne Dague
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    Yeast 24:229-37. 2007
    ....
  35. ncbi request reprint Fusogenic tilted peptides induce nanoscale holes in supported phosphatidylcholine bilayers
    Karim El Kirat
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    Langmuir 21:3116-21. 2005
    ..A molecular mechanism underlying spontaneous insertion of the SIV peptide within lipid bilayers and the subsequent removal of bilayer patches is proposed, and its relevance to membrane fusion processes is discussed...
  36. pmc High-resolution cell surface dynamics of germinating Aspergillus fumigatus conidia
    Etienne Dague
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Louvain la Neuve, Belgium
    Biophys J 94:656-60. 2008
    ..This study illustrates the potential of real-time atomic force microscopy imaging and force spectroscopy for tracking cell-surface dynamics...
  37. doi request reprint Combined use of atomic force microscopy, X-ray photoelectron spectroscopy, and secondary ion mass spectrometry for cell surface analysis
    Etienne Dague
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    Langmuir 24:2955-9. 2008
    ..fumigatus. The results demonstrate the power of using multiple complementary techniques for probing microbial cell surfaces...
  38. ncbi request reprint Using nanotechniques to explore microbial surfaces
    Yves F Dufrêne
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    Nat Rev Microbiol 2:451-60. 2004
  39. pmc Nanoscale analysis of caspofungin-induced cell surface remodelling in Candida albicans
    Sofiane El-Kirat-Chatel
    Universite Catholique de Louvain, Institute of Life Sciences and Institute of Condensed Matter and Nanosciences, Croix du Sud, 1, bte L7 04 01, B 1348 Louvain la Neuve, Belgium
    Nanoscale 5:1105-15. 2013
    ..albicans. The results show that AFM opens up new avenues for understanding the molecular bases of microbe-drug interactions and for developing new therapeutic agents...
  40. doi request reprint Towards a nanoscale view of lactic acid bacteria
    Prachi Tripathi
    Universite Catholique de Louvain, Institute of Condensed Matter and Nanosciences, Croix du Sud, 1, bte L7 04 01, B 1348 Louvain la Neuve, Belgium
    Micron 43:1323-30. 2012
    ..In parallel, single-molecule force spectroscopy has been used to localize and force probe single cell surface constituents, providing novel insights into their spatial distribution and molecular elasticity...
  41. pmc Dual role for the O-acetyltransferase OatA in peptidoglycan modification and control of cell septation in Lactobacillus plantarum
    Elvis Bernard
    Institut National de la Recherche Agronomique, UMR Micalis, Jouy en Josas, France
    PLoS ONE 7:e47893. 2012
    ..Altogether, the results suggest that OatA plays a key role in the spatio-temporal control of septation, irrespective of its catalytic activity...
  42. doi request reprint Atomic force microscopy demonstrates that disulfide bridges are required for clustering of the yeast cell wall integrity sensor Wsc1
    Vincent Dupres
    Institute of Condensed Matter and Nanosciences, Universite Catholique de Louvain, Place Croix du Sud 1, B 1348 Louvain la Neuve, Belgium
    Langmuir 27:15129-34. 2011
    ..Our combined method of AFM with genetic manipulations offers great prospects to explore the mechanisms underlying the clustering of cell surface proteins...
  43. doi request reprint The Pseudomonas aeruginosa membranes: a target for a new amphiphilic aminoglycoside derivative?
    Myriam Ouberai
    Universite Catholique de Louvain, Louvain Drug Research Institute, Unité de Pharmacologie Cellulaire et Moléculaire, UCL 73 70, Avenue E Mounier 73, B 1200 Bruxelles, Belgium
    Biochim Biophys Acta 1808:1716-27. 2011
    ..All together, the data showed that naphthylmethyl neamine derivatives target the membrane of P. aeruginosa. This should offer promising prospects in the search for new antibacterials against drug- or biocide-resistant strains...
  44. pmc Unraveling the nanoscale surface properties of chitin synthase mutants of Aspergillus fumigatus and their biological implications
    David Alsteens
    Universite Catholique de Louvain, Institute of Life Sciences, Louvain la Neuve, Belgium
    Biophys J 105:320-7. 2013
    ..fumigatus and demonstrate the power of atomic force microscopy in fungal genetic studies for assessing the phenotypic characteristics of mutants altered in cell surface organization...
  45. pmc Force-induced formation and propagation of adhesion nanodomains in living fungal cells
    David Alsteens
    Institute of Condensed Matter and Nanosciences, Universite Catholique de Louvain, B 1348 Louvain la Neuve, Belgium
    Proc Natl Acad Sci U S A 107:20744-9. 2010
    ..These results demonstrate that mechanical stimuli can trigger adhesion nanodomains in fungal cells and suggest that the force-induced clustering of adhesins may be a mechanism for activating cell adhesion...
  46. ncbi request reprint Probing molecular recognition sites on biosurfaces using AFM
    Vincent Dupres
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    Biomaterials 28:2393-402. 2007
    ..In this review, we describe the general principles of the AFM technique, present procedures commonly used to prepare samples and tips, and discuss a number of applications that are relevant to the field of biomaterials...
  47. pmc Direct observation of Staphylococcus aureus cell wall digestion by lysostaphin
    Gregory Francius
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Louvain la Neuve, Belgium
    J Bacteriol 190:7904-9. 2008
    ..This study provides new insight into the lytic activity of lysostaphin and offers promising prospects for the study of new antistaphylococcal agents...
  48. doi request reprint Frontiers in microbial nanoscopy
    David Alsteens
    Institute of Condensed Matter and Nanosciences, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    Nanomedicine (Lond) 6:395-403. 2011
    ..In the future, AFM-based nanoscopy should have an important impact on nanomedicine, particularly for understanding microbe-drug and microbe-host interactions, and for developing new antimicrobial strategies...
  49. pmc Imaging the nanoscale organization of peptidoglycan in living Lactococcus lactis cells
    Guillaume Andre
    Institute of Condensed Matter and Nanosciences Bio and Soft Matter, Universite Catholique de Louvain, Croix du Sud 2 18, Louvain la Neuve B 1348, Belgium
    Nat Commun 1:27. 2010
    ..The non-invasive live cell experiments presented here open new avenues for understanding the architecture and assembly of peptidoglycan in Gram-positive bacteria...
  50. ncbi request reprint Probing the interaction forces between hydrophobic peptides and supported lipid bilayers using AFM
    Guillaume Andre
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, 1348 Louvain la Neuve, Belgium
    J Mol Recognit 20:538-45. 2007
    ....
  51. doi request reprint Atomic force microscopy of supported lipid bilayers
    Marie Paule Mingeot-Leclercq
    Unité de Pharmacologie Cellulaire et Moléculaire, Universite Catholique de Louvain, Brussels, Belgium
    Nat Protoc 3:1654-9. 2008
    ..The entire protocol can be completed in 10 h...
  52. doi request reprint Direct measurement of Mycobacterium-fibronectin interactions
    Claire Verbelen
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348, Louvain la Neuve, Belgium
    Integr Biol (Camb) 1:296-300. 2009
    ..The nanoscale analyses presented here complement traditional proteomic and molecular biology approaches for the functional analysis of surface-associated proteins, and may help in the search for novel anti-adhesive drugs...
  53. doi request reprint Forces driving the attachment of Staphylococcus epidermidis to fibrinogen-coated surfaces
    Philippe Herman
    Institute of Life Sciences, Universite Catholique de Louvain, Croix du Sud 1, bte L7 04 01, B 1348 Louvain la Neuve, Belgium
    Langmuir 29:13018-22. 2013
    ..Collectively, our results are consistent with a dynamic, multi-step ligand-binding mechanism called "dock, lock, and latch"...
  54. ncbi request reprint Antigen binding forces of single antilysozyme Fv fragments explored by atomic force microscopy
    Alexandre Berquand
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    Langmuir 21:5517-23. 2005
    ..The kinetic off-rate constant of dissociation (k(off) approximately = 1 x 10(-3) s(-1)) obtained by extrapolating the data of the low-strength regime to zero force was in the range of the k(off) estimated by SPR...
  55. pmc Binding mechanism of the peptidoglycan hydrolase Acm2: low affinity, broad specificity
    Audrey Beaussart
    Universite Catholique de Louvain, Institute of Life Sciences, Croix du Sud, Louvain la Neuve, Belgium
    Biophys J 105:620-9. 2013
    ..The binding mechanism (low affinity and broad specificity) of Acm2 may represent a generic mechanism among cell-wall hydrolases for guiding cell division and cell adhesion...
  56. doi request reprint Nanoscale imaging of the Candida-macrophage interaction using correlated fluorescence-atomic force microscopy
    Sofiane El-Kirat-Chatel
    Institute of Life Sciences, Universite Catholique de Louvain, Croix du Sud, 1, bte L7 04 01, B 1348 Louvain la Neuve, Belgium
    ACS Nano 6:10792-9. 2012
    ..As fungal pathogenesis is mainly controlled by the ability of fungi to escape from immune cells, the nanoimaging platform established here has great potential in nanomedicine for understanding and controlling fungal infections...
  57. doi request reprint Atomic force microscopy: a powerful tool for studying bacterial swarming motility
    Annika Gillis
    Laboratory of Food and Environmental Microbiology, Earth and Life Institute, Universite Catholique de Louvain, Croix du Sud 2, Box L7 05 12, B 1348 Louvain la Neuve, Belgium
    Micron 43:1304-11. 2012
    ..This study shows that AFM imaging can readily discriminate between swarming and non-swarming cells, and quantify their morphological details, thus offering an important tool to study the dynamics of bacterial populations...
  58. pmc Detection and localization of single LysM-peptidoglycan interactions
    Guillaume Andre
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    J Bacteriol 190:7079-86. 2008
    ..These results provide novel insight into the binding forces of bacterial LysMs and show that SMFS is a promising tool for studying the heterologous display of proteins or peptides on bacterial surfaces...
  59. ncbi request reprint Single-molecule force spectroscopy and imaging of the vancomycin/D-Ala-D-Ala interaction
    Yann Gilbert
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    Nano Lett 7:796-801. 2007
    ..The single-molecule approach presented here provides new opportunities for understanding the binding mechanisms of antibiotics and for exploring the architecture of bacterial cell walls...
  60. ncbi request reprint Aggregation of yeast cells: direct measurement of discrete lectin-carbohydrate interactions
    Ahmed Touhami
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    Microbiology 149:2873-8. 2003
    ..The single molecule force spectroscopy measurements presented here provide a means to study a variety of cellular interactions at the molecular level, such as the adhesion of bacteria to animal and plant tissues...
  61. pmc Atomic force microscopy, a powerful tool in microbiology
    Yves F Dufrêne
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, B 1348 Louvain la Neuve, Belgium
    J Bacteriol 184:5205-13. 2002
  62. pmc Unzipping a functional microbial amyloid
    David Alsteens
    Institute of Life Sciences and Institute of Condensed Matter and Nanosciences, Universite Catholique de Louvain, Croix du Sud, 1 bte L7 04 01, B 1348 Louvain la Neuve, Belgium
    ACS Nano 6:7703-11. 2012
    ..In nanotechnology, these single-molecule manipulation experiments provide new opportunities to understand the molecular mechanisms driving the cohesion of functional amyloid-based nanostructures...
  63. ncbi request reprint Nanoscale chemical patterns fabricated by using colloidal lithography and self-assembled monolayers
    Frédéric A Denis
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    Langmuir 20:9335-9. 2004
    ....
  64. ncbi request reprint Real-time imaging of the surface topography of living yeast cells by atomic force microscopy
    Francois Ahimou
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    Yeast 20:25-30. 2003
    ..These results indicate that AFM is a complementary tool to electron microscopy in that it allows the surface of living cells to be explored directly in real time...
  65. doi request reprint Nanoscale imaging of Bacillus thuringiensis flagella using atomic force microscopy
    Annika Gillis
    Laboratory of Food and Environmental Microbiology, Earth and Life Institute, Universite Catholique de Louvain, Croix du Sud 2, Box L7 05 12, B 1348, Louvain la Neuve, Belgium
    Nanoscale 4:1585-91. 2012
    ....
  66. doi request reprint Measuring cell wall thickness in living yeast cells using single molecular rulers
    Vincent Dupres
    Institute of Condensed Matter and Nanosciences, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    ACS Nano 4:5498-504. 2010
    ..The single molecular ruler method presented here not only avoids cell fixation artifacts but also provides new opportunities for studying the dynamics of microbial cell walls during growth, drug action, or enzymatic modification...
  67. pmc Characterization of the interactions between fluoroquinolone antibiotics and lipids: a multitechnique approach
    Hayet Bensikaddour
    Universite Catholique de Louvain, Faculty of Medicine, Unité de Pharmacologie Cellulaire et Moléculaire, Brussels, Belgium
    Biophys J 94:3035-46. 2008
    ..All together, differences of ciprofloxacin as compared to moxifloxacin in their interactions with lipids could explain differences in their cellular accumulation and susceptibility to efflux transporters...
  68. ncbi request reprint The NTA-His6 bond is strong enough for AFM single-molecular recognition studies
    Claire Verbelen
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    J Mol Recognit 20:490-4. 2007
    ..We conclude that the NTA-His6 system is a powerful, well-suited platform for the stable, oriented immobilization of proteins in AFM single-molecule studies...
  69. ncbi request reprint Structure, cell wall elasticity and polysaccharide properties of living yeast cells, as probed by AFM
    David Alsteens
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    Nanotechnology 19:384005. 2008
    ..This study demonstrates the power of using multiple complementary AFM modalities for probing the organization and interactions of the various macromolecules of microbial cell walls...
  70. ncbi request reprint Nanoscale exploration of microbial surfaces using the atomic force microscope
    Yves F Dufrêne
    Universite Catholique de Louvain, Unité de chimie des interfaces Nanobio team, Croix du Sud 2 18, Louvain la Neuve, Belgium
    Future Microbiol 1:387-96. 2006
    ....
  71. doi request reprint Controlled manipulation of bacteriophages using single-virus force spectroscopy
    David Alsteens
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    ACS Nano 3:3063-8. 2009
    ....
  72. doi request reprint Nanostructure and nanomechanics of live Phaeodactylum tricornutum morphotypes
    Gregory Francius
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgique
    Environ Microbiol 10:1344-56. 2008
    ..Last, we observed mucilaginous footprints of moderate stiffness (approximately 100 kPa) in the vicinity of ovoid diatoms, which we believe are secreted extracellular polymers...
  73. pmc Refining our perception of bacterial surfaces with the atomic force microscope
    Yves F Dufrêne
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, B 1348 Louvain la Neuve, Belgium
    J Bacteriol 186:3283-5. 2004
  74. pmc Unfolding individual als5p adhesion proteins on live cells
    David Alsteens
    Unité de Chimie des Interfaces, Université catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    ACS Nano 3:1677-82. 2009
    ....
  75. ncbi request reprint Blistering of supported lipid membranes induced by Phospholipase D, as observed by real-time atomic force microscopy
    Karim El Kirat
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    Biochim Biophys Acta 1778:276-82. 2008
    ..These results point to the importance of lipid packing on PLD activity and illustrate the potential of AFM for visualizing remodeling enzymatic activities...
  76. ncbi request reprint Exploring the molecular forces within and between CbsA S-layer proteins using single molecule force spectroscopy
    Claire Verbelen
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    Ultramicroscopy 107:1004-11. 2007
    ..We expect that the intra- and intermolecular forces measured here may play a significant role in controlling the stability and assembly of the CbsA protein...
  77. ncbi request reprint Recent progress in AFM molecular recognition studies
    Yves F Dufrêne
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, 1348 Louvain la Neuve, Belgium
    Pflugers Arch 456:237-45. 2008
    ..In this review, we describe the principles of molecular recognition studies using AFM and provide a flavor of recent progress made in the field...
  78. ncbi request reprint Recent progress in the application of atomic force microscopy imaging and force spectroscopy to microbiology
    Yves F Dufrêne
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    Curr Opin Microbiol 6:317-23. 2003
    ....
  79. ncbi request reprint Nanoscale mapping and functional analysis of individual adhesins on living bacteria
    Vincent Dupres
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    Nat Methods 2:515-20. 2005
    ..We then mapped the distribution of single HBHA molecules on the surface of living mycobacteria and found that the adhesin is not randomly distributed over the mycobacterial surface, but concentrated into nanodomains...
  80. doi request reprint Five challenges to bringing single-molecule force spectroscopy into living cells
    Yves F Dufrêne
    Universite Catholique de Louvain, Institute of Condensed Matter and Nanosciences, Louvain la Neuve, Belgium
    Nat Methods 8:123-7. 2011
    ..Here we discuss the problems and challenges that need to be addressed to bring these technologies into living cells and to learn how cellular machinery is controlled in vivo...