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Genomes and Genes | Lucien BettendorffSummaryCountry: Belgium Publications
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Thiamin diphosphate in biological chemistry: new aspects of thiamin metabolism, especially triphosphate derivatives acting other than as cofactorsLucien Bettendorff
GIGA Neurosciences, University of Liege, Belgium
FEBS J 276:2917-25. 2009..coli, but the mechanism of ThTP synthesis remains elusive. The data reviewed here illustrate the complexity of thiamin biochemistry, which is not restricted to the cofactor role of ThDP...
A general method for the chemical synthesis of gamma-32P-labeled or unlabeled nucleoside 5(')-triphosphates and thiamine triphosphateLucien Bettendorff
Center for Cellular and Molecular Neurobiology, University of Liege, 17, place Delcour, 4020, Liege, Belgium
Anal Biochem 322:190-7. 2003..Our method can easily be scaled up for industrial synthesis of a variety of labeled and unlabeled triphosphoric derivatives from their mono- or diphosphate precursors...- Discovery of a natural thiamine adenine nucleotideLucien Bettendorff
Center for Cellular and Molecular Neurobiology, University of Liege, Avenue de l Hopital 1, B 4000 Liege, Belgium
Nat Chem Biol 3:211-2. 2007..We discovered AThTP in Escherichia coli and found that it accumulates specifically in response to carbon starvation, thereby acting as a signal rather than a cofactor. We detected smaller amounts in yeast and in plant and animal tissues... - Pig tissues express a catalytically inefficient 25-kDa thiamine triphosphatase: insight in the catalytic mechanisms of this enzymePiotr Szyniarowski
Center for Cellular and Molecular Neurobiology, University of Liege, B 4000 Liege, Belgium
Biochim Biophys Acta 1725:93-102. 2005..This raises the possibility that this protein may play a physiological role other than ThTP hydrolysis... 
Adenosine thiamine triphosphate accumulates in Escherichia coli cells in response to specific conditions of metabolic stressTiziana Gigliobianco
GIGA Neurosciences, University of Liege, Liege, Belgium
BMC Microbiol 10:148. 2010..Here, we address the question whether AThTP accumulation in E. coli is triggered by the absence of a carbon source in the medium, the resulting drop in energy charge or other forms of metabolic stress...- High inorganic triphosphatase activities in bacteria and mammalian cells: identification of the enzymes involvedGregory Kohn
GIGA Neurosciences, University of Liege, Liege, Belgium
PLoS ONE 7:e43879. 2012..The aim of the present study is to determine whether other members of the CYTH protein family also have a PPPase activity, if there are PPPase activities in animal tissues and what enzymes are responsible for these activities... - Human recombinant thiamine triphosphatase: purification, secondary structure and catalytic propertiesBernard Lakaye
Center for Cellular and Molecular Neurobiology, University of Liege, 17 Place Delcour, B 4020 Liege, Belgium
Int J Biochem Cell Biol 36:1348-64. 2004..The secondary structure of the enzyme, as determined by Fourier-transform infrared spectroscopy, was predominantly beta-sheet and alpha-helix... - Thiamine triphosphate, a new signal required for optimal growth of Escherichia coli during amino acid starvationBernard Lakaye
Center for Cellular and Molecular Neurobiology, University of Liege, 17, place Delcour, 4020 Liege, Belgium
J Biol Chem 279:17142-7. 2004..This is the first demonstration of a physiological role of this ubiquitous compound in any organism... - Thiamine triphosphate synthesis in rat brain occurs in mitochondria and is coupled to the respiratory chainMarjorie Gangolf
GIGA Neurosciences B36, University of Liege, Avenue de l Hopital 1, B 4000 Liege 1 Sart Tilman, Belgium B 4000 Liège, Belgium
J Biol Chem 285:583-94. 2010..This might shed a new light on our understanding of the mechanisms of thiamine deficiency-induced brain lesions... - Adenylate kinase-independent thiamine triphosphate accumulation under severe energy stress in Escherichia coliTiziana Gigliobianco
Center for Cellular and Molecular Neurobiology, University of Liege, Liege, Belgium
BMC Microbiol 8:16. 2008..However, it is also known that vertebrate adenylate kinase 1 catalyzes ThTP synthesis at a very low rate and it has been postulated that this enzyme is responsible for ThTP synthesis in vivo... - Expression of 25 kDa thiamine triphosphatase in rodent tissues using quantitative PCR and characterization of its mRNABernard Lakaye
Center for Cellular and Molecular Neurobiology, University of Liege, 17 Place Delcour, 4020 Liege, Belgium
Int J Biochem Cell Biol 36:2032-41. 2004..This is the first study demonstrating a cell-specific 25 kDa ThTPase mRNA expression, suggesting that this enzyme might be related to the degree of differentiation or the metabolic state of the cell... - Thiamine diphosphate adenylyl transferase from E. coli: functional characterization of the enzyme synthesizing adenosine thiamine triphosphateAlexander F Makarchikov
Center for Cellular and Molecular Neurobiology, University of Liege, Liege, Belgium
BMC Biochem 8:17. 2007..Thus, we hypothesized that AThTP may be a signal produced in response to carbon starvation... - Bisindole alkaloids from Strychnos guianensis are effective antagonists of nicotinic acetylcholine receptors in cultured human TE671 cellsPierre Wins
Center for Cellular and Molecular Neurobiology, , place Delcour, 17, , Belgium
Naunyn Schmiedebergs Arch Pharmacol 367:253-9. 2003..The competitive component (K(I)=0.21 microM) is presumably due to binding at the acetylcholine site, while the uncompetitive component (K'(I)=0.92 microM) may be due to open channel block... - Thiamine status in humans and content of phosphorylated thiamine derivatives in biopsies and cultured cellsMarjorie Gangolf
GIGA Neurosciences, University of Liege, Liege, Belgium
PLoS ONE 5:e13616. 2010..This is the first comprehensive study on the distribution of thiamine derivatives in human biopsies, body fluids and cell lines... - An alternative role of FoF1-ATP synthase in Escherichia coli: synthesis of thiamine triphosphateTiziana Gigliobianco
Unit of Bioenergetics and cerebral Excitability, GIGA Neurosciences, University of Liege, Liege, Belgium
Sci Rep 3:1071. 2013..Moreover, this chemiosmotic mechanism for ThTP production is conserved from E. coli to mammalian brain mitochondria... - Molecular characterization of a specific thiamine triphosphatase widely expressed in mammalian tissuesBernard Lakaye
Center for Cellular and Molecular Neurobiology, Department of Human Histology, University of Liege, 4020 Liege, Belgium
J Biol Chem 277:13771-7. 2002..The mRNA was expressed in most human tissues but at relatively low levels. This is the first report of a molecular characterization of a specific ThTPase... - ATP-driven, Na(+)-independent inward Cl- pumping in neuroblastoma cellsLucien Bettendorff
Center for Cellular and Molecular Neurobiology, University of Liege, 17 Place Delcour, B 4020 Liege, Belgium
J Neurochem 81:792-801. 2002..These results suggest that a highly active ATP-dependent mechanism, distinct from Na(+) -K(+) -2Cl(-) co-transport, is responsible for most of the inward Cl- pumping in N2a cells... - A specific inorganic triphosphatase from Nitrosomonas europaea: structure and catalytic mechanismDavid Delvaux
GIGA Neuroscience, University of Liege, B 4000 Liege, Belgium
J Biol Chem 286:34023-35. 2011..The present data provide the first characterization of a new type of phosphohydrolase (unrelated to pyrophosphatases or exopolyphosphatases), able to hydrolyze inorganic triphosphate with high specificity... - Adenylate kinase 1 knockout mice have normal thiamine triphosphate levelsAlexander F Makarchikov
Center for Cellular and Molecular Neurobiology, University of Liege, 17 Place Delcour, Belgium
Biochim Biophys Acta 1592:117-21. 2002.... - Benfotiamine, a synthetic S-acyl thiamine derivative, has different mechanisms of action and a different pharmacological profile than lipid-soluble thiamine disulfide derivativesMarie Laure Volvert
Center for Cellular and Molecular Neurobiology, University of Liege, Avenue de l Hopital, 1, 4000 Liege, Belgium
BMC Pharmacol 8:10. 2008..As the brain is particularly sensitive to thiamine deficiency, we wanted to test whether intracellular thiamine and thiamine phosphate levels are increased in the brain after oral benfotiamine administration... - Overexpression of CD39 in mouse airways promotes bacteria-induced inflammationEmilie Theatre
Interdisciplinary Cluster of Applied Genoproteomics Inflammation, Infection, Immunity, Unit of Human Genetics, Laboratory of Thrombosis and Haemostasis, University of Liege, Liege 4000, Belgium
J Immunol 189:1966-74. 2012..Thus, a chronic increase of epithelial CD39 expression and activity promotes airway inflammation in response to bacterial challenge by enhancing P1 and P2 receptor activation... - Autocrine/paracrine activation of the GABA(A) receptor inhibits the proliferation of neurogenic polysialylated neural cell adhesion molecule-positive (PSA-NCAM+) precursor cells from postnatal striatumLaurent Nguyen
Center for Cellular and Molecular Neurobiology, University of Liege, B 4020 Liege, Belgium
J Neurosci 23:3278-94. 2003..This demonstration of GABA synthesis and GABA(A)R function in striatal PSA-NCAM+ cells may shed new light on the understanding of key extrinsic cues that regulate the developmental potential of postnatal neuronal precursors in the CNS... - At the crossroad of thiamine degradation and biosynthesisLucien Bettendorff
Nat Chem Biol 3:454-5. 2007 - Structural basis for the catalytic mechanism of mammalian 25-kDa thiamine triphosphataseJikui Song
Center for Eukaryotic Structural Genomics and National Magnetic Resonance Facility at Madison, Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706 1544, USA
J Biol Chem 283:10939-48. 2008..We propose a mechanism underlying the high substrate specificity of mThTPase and discuss the possible role of water molecules in enzymatic catalysis...