W Becker

Summary

Publications

  1. ncbi Fluorescence lifetime images and correlation spectra obtained by multidimensional time-correlated single photon counting
    W Becker
    Becker and Hickl GmbH, 12277 Berlin, Germany
    Microsc Res Tech 69:186-95. 2006
  2. ncbi Fluorescence lifetime imaging by time-correlated single-photon counting
    W Becker
    Becker and Hickl GmbH, D 12277 Berlin, Germany
    Microsc Res Tech 63:58-66. 2004
  3. ncbi Sequence characteristics, subcellular localization, and substrate specificity of DYRK-related kinases, a novel family of dual specificity protein kinases
    W Becker
    Institut fur Pharmakologie und Toxikologie, RWTH Aachen, D 52057 Aachen, Germany
    J Biol Chem 273:25893-902. 1998
  4. ncbi Cloning and characterization of DYRK1B, a novel member of the DYRK family of protein kinases
    S Leder
    Institut fur Pharmakologie und Toxikologie, RWTH Aachen, Aachen, D 52057, Germany
    Biochem Biophys Res Commun 254:474-9. 1999
  5. ncbi Dyrk, a dual specificity protein kinase with unique structural features whose activity is dependent on tyrosine residues between subdomains VII and VIII
    H Kentrup
    Institut fur Pharmakologie und Toxikologie, Rheinisch Westfälische Technische Hochschule Aachen, D 52057 Aachen, Federal Republic of Germany
    J Biol Chem 271:3488-95. 1996
  6. ncbi STAT3 is enriched in nuclear bodies
    Andreas Herrmann
    Institut fur Biochemie, Universitätsklinikum RWTH Aachen, Pauwelsstrasse 30, 52057 Aachen, Germany
    J Cell Sci 117:339-49. 2004
  7. ncbi Identification of the autophosphorylation sites and characterization of their effects in the protein kinase DYRK1A
    S Himpel
    , , Wendlingweg 2, D-52057 Aachen, Germany
    Biochem J 359:497-505. 2001
  8. ncbi The kinase DYRK1A phosphorylates the transcription factor FKHR at Ser329 in vitro, a novel in vivo phosphorylation site
    Y L Woods
    MRC Protein Phosphorylation Unit, MSI WTB complex, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, UK
    Biochem J 355:597-607. 2001
  9. ncbi cDNA cloning and characterization of rat Clk3, a LAMMER kinase predominately expressed in testis
    W Becker
    Institut fur Pharmakologie und Toxikologie, Medizinische Fakultat, RWTH Aachen, Germany
    Biochim Biophys Acta 1312:63-7. 1996
  10. ncbi Molecular cloning and characterization of a novel mammalian protein kinase harboring a homology domain that defines a subfamily of serine/threonine kinases
    W Becker
    Institut für Pharmakologie und Toxikologie der RWTH Aachen, Germany
    Eur J Biochem 235:736-43. 1996

Collaborators

  • K Konig
  • P Schwille
  • L Kelbauskas
  • P Cohen
  • A Ruck
  • Andreas Herrmann
  • H G Joost
  • H Kentrup
  • Y L Woods
  • S Himpel
  • S Leder
  • I Kinzler
  • Ch Hülshoff
  • R Steiner
  • Peter C Heinrich
  • Gerhard Muller-Newen
  • Serge Haan
  • Albert L Pranada
  • Ulrike Sommer
  • Bernd Giese
  • Andrea Küster
  • S Guo
  • L C Packman
  • T G Unterman
  • N Morrice
  • A Barthel
  • G Rena
  • M Sayed
  • K Eirmbter
  • P Panzer
  • T Blundell
  • H Czajkowska
  • X Altafaj
  • Y Weber
  • X Estivill
  • J Heukelbach
  • C Huppertz
  • A Wilmes
  • A Schurmann
  • H Kainulainen

Detail Information

Publications11

  1. ncbi Fluorescence lifetime images and correlation spectra obtained by multidimensional time-correlated single photon counting
    W Becker
    Becker and Hickl GmbH, 12277 Berlin, Germany
    Microsc Res Tech 69:186-95. 2006
    ..We show that the same technique and the same hardware can be used for precision fluorescence decay analysis and fluorescence correlation spectroscopy (FCS) in selected spots of a sample...
  2. ncbi Fluorescence lifetime imaging by time-correlated single-photon counting
    W Becker
    Becker and Hickl GmbH, D 12277 Berlin, Germany
    Microsc Res Tech 63:58-66. 2004
    ..The technique can be used with almost any confocal or two-photon laser scanning microscope and works at any scanning rate. We demonstrate the application to samples stained with several dyes and to CFP-YFP FRET...
  3. ncbi Sequence characteristics, subcellular localization, and substrate specificity of DYRK-related kinases, a novel family of dual specificity protein kinases
    W Becker
    Institut fur Pharmakologie und Toxikologie, RWTH Aachen, D 52057 Aachen, Germany
    J Biol Chem 273:25893-902. 1998
    ..The heterogeneity of their subcellular localization and substrate specificity suggests that the kinases are involved in different cellular functions...
  4. ncbi Cloning and characterization of DYRK1B, a novel member of the DYRK family of protein kinases
    S Leder
    Institut fur Pharmakologie und Toxikologie, RWTH Aachen, Aachen, D 52057, Germany
    Biochem Biophys Res Commun 254:474-9. 1999
    ..These data suggest that DYRK1B is a muscle- and testis-specific isoform of DYRK1A and is involved in the regulation of nuclear functions...
  5. ncbi Dyrk, a dual specificity protein kinase with unique structural features whose activity is dependent on tyrosine residues between subdomains VII and VIII
    H Kentrup
    Institut fur Pharmakologie und Toxikologie, Rheinisch Westfälische Technische Hochschule Aachen, D 52057 Aachen, Federal Republic of Germany
    J Biol Chem 271:3488-95. 1996
    ..The data suggest that Dyrk is a dual specificity protein kinase that is regulated by tyrosine phosphorylation in the activation loop and might be a component of a signaling pathway regulating nuclear functions...
  6. ncbi STAT3 is enriched in nuclear bodies
    Andreas Herrmann
    Institut fur Biochemie, Universitätsklinikum RWTH Aachen, Pauwelsstrasse 30, 52057 Aachen, Germany
    J Cell Sci 117:339-49. 2004
    ..Taken together, our data suggest that STAT3 nuclear bodies are either directly involved in active gene transcription or they serve as reservoirs of activated STAT3...
  7. ncbi Identification of the autophosphorylation sites and characterization of their effects in the protein kinase DYRK1A
    S Himpel
    , , Wendlingweg 2, D-52057 Aachen, Germany
    Biochem J 359:497-505. 2001
    ..The present experimental data and the molecular model indicate that the activity of DYRK1A is dependent on the autophosphorylation of a conserved tyrosine residue in the activation loop...
  8. ncbi The kinase DYRK1A phosphorylates the transcription factor FKHR at Ser329 in vitro, a novel in vivo phosphorylation site
    Y L Woods
    MRC Protein Phosphorylation Unit, MSI WTB complex, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, UK
    Biochem J 355:597-607. 2001
    ..We find that FKHR and DYRK1A co-localize in discrete regions of the nucleus and can be co-immunoprecipitated from cell extracts. These experiments suggest that DYRK1A may phosphorylate FKHR at Ser(329) in vivo...
  9. ncbi cDNA cloning and characterization of rat Clk3, a LAMMER kinase predominately expressed in testis
    W Becker
    Institut fur Pharmakologie und Toxikologie, Medizinische Fakultat, RWTH Aachen, Germany
    Biochim Biophys Acta 1312:63-7. 1996
    ..By Northern blot analysis of different rat tissues, mRNA of Clk3 was detected predominately in testis, suggesting that this kinase regulates a predominately testicular function...
  10. ncbi Molecular cloning and characterization of a novel mammalian protein kinase harboring a homology domain that defines a subfamily of serine/threonine kinases
    W Becker
    Institut für Pharmakologie und Toxikologie der RWTH Aachen, Germany
    Eur J Biochem 235:736-43. 1996
    ..This domain (designated the SNH domain) appears to define the subfamily of SNF1-related protein kinases and might represent a new type of regulatory domain of protein kinases...
  11. ncbi SLIM: a new method for molecular imaging
    A Ruck
    Institute for Laser Technologies in Medicine and Metrology, Helmholtzstr 12, 89081 Ulm, Germany
    Microsc Res Tech 70:485-92. 2007
    ..Different lifetimes could be attributed to the various compounds. In addition, a detailed analysis of the autofluorescence by SLIM could explain changes of mitochondrial metabolism during Photofrin-PDT...