Functional evaluation of primary renal cell/biomaterial neo-kidney augment prototypes for renal tissue engineeringJoydeep Basu
Tengion Inc, Winston Salem, NC 27103, USA
Cell Transplant 20:1771-90. 2011
..Conditioned medium was observed to attenuate TGF-β-induced epithelial-mesenchymal transition (EMT) in vitro in a human proximal tubular cell line (HK2)...
- Regeneration of rodent small intestine tissue following implantation of scaffolds seeded with a novel source of smooth muscle cells
Joydeep Basu
Tengion Inc, 3929 Westpoint Boulevard, Suite G, Winston Salem, NC 27103, USA
Regen Med 6:721-31. 2011
..To apply an organ regeneration platform technology of autologous smooth muscle cell/biomaterial combination products, previously demonstrated to be successful for urinary tissue regeneration, to the regeneration of the small intestine...
- Regeneration of native-like neo-urinary tissue from nonbladder cell sources
Joydeep Basu
Bioprocess Research and Assay Development, Tengion Inc, Winston Salem, North Carolina 27103, USA
Tissue Eng Part A 18:1025-34. 2012
..The ability to create urologic structures de novo from scaffolds seeded by autologous adipose- or peripheral blood-derived SMCs will greatly facilitate the translation of urologic tissue engineering technologies into clinical practice...
- Extension of bladder-based organ regeneration platform for tissue engineering of esophagus
Joydeep Basu
Tengion Inc, 3929 Westpoint Blvd, Suite G, Winston Salem, NC 27103, USA
Med Hypotheses 78:231-4. 2012
..By implication, such regenerative constructs may potentially be used to mediate the regeneration of any laminarly organized tubular organ...
- Platform technologies for tubular organ regeneration
Joydeep Basu
Bioprocess Research and Assay Development, Tengion Inc, 3929 Westpoint Blvd, Suite G, Winston Salem, NC 27103, USA
Trends Biotechnol 28:526-33. 2010
..We focus on identifying the elements of this platform that have facilitated the transition of tubular organ regeneration from academic proof-of-concept to commercial viability...
- Expansion of the human adipose-derived stromal vascular cell fraction yields a population of smooth muscle-like cells with markedly distinct phenotypic and functional properties relative to mesenchymal stem cells
Joydeep Basu
Bioprocess Research and Assay Development, Tengion Inc, Winston Salem, North Carolina 27103, USA
Tissue Eng Part C Methods 17:843-60. 2011
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Organ specific regenerative markers in peri-organ adipose: kidneyJoydeep Basu
Bioprocess Research and Assay Development, Tengion Inc, 3929 Westpoint Blvd, Suite G, Winston Salem, NC 27103, USA
Lipids Health Dis 10:171. 2011
..To characterize this topographical association, we explored the potential of cells isolated from the stromal vascular fraction (SVF) of kidney sourced adipose to express key renal associated factors...
- Increased urothelial cell detection in the primary bladder smooth muscle cell cultures with dual MACS/qRT-PCR approach
Chistopher W Genheimer
Bioprocess Research and Assay Development, Tengion Inc, Winston Salem, NC 27103, USA
Appl Immunohistochem Mol Morphol 19:184-9. 2011
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- Smooth muscle phenotypic diversity is mediated through alterations in myocardin gene splicing
Roger M Ilagan
Tengion, Inc, Science and Technology, Winston Salem, North Carolina, USA
J Cell Physiol 226:2702-11. 2011
..Together, these data suggest that MYOCD ΔExon 11 may participate in modulating SMC phenotype, potentially acting as a dominant-negative repressor of contraction-related genes...
- Tissue engineering of esophagus and small intestine in rodent injury models
Joydeep Basu
Tengion Inc, Winston Salem, NC, USA
Methods Mol Biol 1001:311-24. 2013
..Here, we describe how such constructs may be applied to catalyze regeneration of esophagus and small intestine in preclinical rodent models...
Molecular characterization of the regenerative response induced by intrarenal transplantation of selected renal cells in a rodent model of chronic kidney diseaseChristopher W Genheimer
Bioprocess Research and Assay Development, Tengion, Inc, Winston Salem, NC 27103, USA
Cells Tissues Organs 196:374-84. 2012
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- Developmental engineering the kidney: leveraging principles of morphogenesis for renal regeneration
Joydeep Basu
Tengion, Inc, Winston Salem, North Carolina 27103, USA joydeep
Birth Defects Res C Embryo Today 96:30-8. 2012
..Ultimately, such methodologies may facilitate the establishment of clinically relevant therapeutic strategies for regeneration of renal structure and function, greatly impacting treatment regimens for chronic kidney disease...
- Migration assay to evaluate cellular interactions with biomaterials for tissue engineering/regenerative medicine applications
Kelly I Guthrie
Tengion Inc, Winston Salem, NC, USA
Methods Mol Biol 1001:189-96. 2013
..Principles of tissue engineering, Academic Press, 2007; Mieszawska and Kaplan. BMC Biol 8:59, 2010; Matragotri and Lahann J. Nat Mater 8:15-23, 2009)...
- Linear measurement of cell contraction in a capillary collagen gel system
Roger Ilagan
Tengion, Inc, Bioprocess Research and Development, Winston Salem, NC 27103, USA
Biotechniques 48:153-5. 2010
..In addition, capillary tube gels require significantly fewer cells and far less reagents than standard methods...
- Construction of a tubular scaffold that mimics J-shaped stress/strain mechanics using an innovative electrospinning technique
Harry Scott Rapoport
Bioprocess Research and Assay Development, Tengion, Inc, Winston Salem, NC 27103, USA
Tissue Eng Part C Methods 18:567-74. 2012
..3±1.9 MJ/m(3). The ability to employ different materials and different formation parameters utilizing this technique promises the ability to match complex stress-strain behaviors in soft tissues with a high degree of fidelity...
