Bharati Bapat

Summary

Publications

  1. ncbi request reprint Germline MYH mutations in a clinic-based series of Canadian multiple colorectal adenoma patients
    Marina E Croitoru
    Centre for Cancer Genetics, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    J Surg Oncol 95:499-506. 2007
  2. ncbi request reprint Suppression of nuclear Wnt signaling leads to stabilization of Rac1 isoforms
    Susmita Esufali
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 60 Murray Street, Room L6 304 4, Toronto, Canada
    FEBS Lett 581:4850-6. 2007
  3. doi request reprint Cyclin D1 Pro241Pro (CCND1-G870A) polymorphism is associated with increased cancer risk in human populations: a meta-analysis
    Noel Pabalan
    Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, 60 Murray Street, Room L6 304, Box 29, Toronto, Ontario, Canada M5T 3L9
    Cancer Epidemiol Biomarkers Prev 17:2773-81. 2008
  4. doi request reprint A large novel deletion in the APC promoter region causes gene silencing and leads to classical familial adenomatous polyposis in a Manitoba Mennonite kindred
    George S Charames
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 60 Murray St, Toronto, Box 30, ON, M5T 3L9, Canada
    Hum Genet 124:535-41. 2008
  5. doi request reprint Rac1b recruits Dishevelled and β-catenin to Wnt target gene promoters independent of Wnt3A stimulation
    Vaijayanti V Pethe
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, M5T 3L9, Canada
    Int J Oncol 39:805-10. 2011
  6. pmc Strategies for discovery and validation of methylated and hydroxymethylated DNA biomarkers
    Ekaterina Olkhov-Mitsel
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital Toronto, Ontario, Canada Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada
    Cancer Med 1:237-60. 2012
  7. pmc Novel multiplex MethyLight protocol for detection of DNA methylation in patient tissues and bodily fluids
    Ekaterina Olkhov-Mitsel
    1 Lunenfeld Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, Canada 2 Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada
    Sci Rep 4:4432. 2014
  8. pmc MLH1 region polymorphisms show a significant association with CpG island shore methylation in a large cohort of healthy individuals
    Andrea J Savio
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada
    PLoS ONE 7:e51531. 2012
  9. pmc Discovery of novel hypermethylated genes in prostate cancer using genomic CpG island microarrays
    Ken Kron
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    PLoS ONE 4:e4830. 2009
  10. pmc Specific variants in the MLH1 gene region may drive DNA methylation, loss of protein expression, and MSI-H colorectal cancer
    Miralem Mrkonjic
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada
    PLoS ONE 5:e13314. 2010

Collaborators

Detail Information

Publications47

  1. ncbi request reprint Germline MYH mutations in a clinic-based series of Canadian multiple colorectal adenoma patients
    Marina E Croitoru
    Centre for Cancer Genetics, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    J Surg Oncol 95:499-506. 2007
    ..Our objective was to determine the significance of MYH mutations in a series of Canadian patients with multiple adenomas...
  2. ncbi request reprint Suppression of nuclear Wnt signaling leads to stabilization of Rac1 isoforms
    Susmita Esufali
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 60 Murray Street, Room L6 304 4, Toronto, Canada
    FEBS Lett 581:4850-6. 2007
    ..In addition, we show that Rac1b may be under proteasomal regulation. We propose that Rac1 and Rac1b levels are regulated by being targeted for degradation through a negative feedback loop initiated by Wnt signaling...
  3. doi request reprint Cyclin D1 Pro241Pro (CCND1-G870A) polymorphism is associated with increased cancer risk in human populations: a meta-analysis
    Noel Pabalan
    Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, 60 Murray Street, Room L6 304, Box 29, Toronto, Ontario, Canada M5T 3L9
    Cancer Epidemiol Biomarkers Prev 17:2773-81. 2008
    ..6-7.1). In conclusion, our study strongly supports the increased cancer risk associated with CCND1-A870G polymorphism in the human population...
  4. doi request reprint A large novel deletion in the APC promoter region causes gene silencing and leads to classical familial adenomatous polyposis in a Manitoba Mennonite kindred
    George S Charames
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 60 Murray St, Toronto, Box 30, ON, M5T 3L9, Canada
    Hum Genet 124:535-41. 2008
    ..This study strengthens the likelihood that this novel APC promoter mutation is linked to this unique population as a founder mutation...
  5. doi request reprint Rac1b recruits Dishevelled and β-catenin to Wnt target gene promoters independent of Wnt3A stimulation
    Vaijayanti V Pethe
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, M5T 3L9, Canada
    Int J Oncol 39:805-10. 2011
    ..Our studies suggest a novel transcriptional co-activator role of Rac1b in β-catenin/TCF-mediated transcription...
  6. pmc Strategies for discovery and validation of methylated and hydroxymethylated DNA biomarkers
    Ekaterina Olkhov-Mitsel
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital Toronto, Ontario, Canada Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada
    Cancer Med 1:237-60. 2012
    ..The development of DNA methylation- and hydroxymethylation-based biomarkers is an exciting and rapidly evolving area of research that holds promise for potential applications in diverse clinical settings...
  7. pmc Novel multiplex MethyLight protocol for detection of DNA methylation in patient tissues and bodily fluids
    Ekaterina Olkhov-Mitsel
    1 Lunenfeld Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, Canada 2 Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada
    Sci Rep 4:4432. 2014
    ..These data show that the multiplex MethyLight assay offers a significant advantage when working with limited quantities of DNA and has potential applications in research and clinical settings. ..
  8. pmc MLH1 region polymorphisms show a significant association with CpG island shore methylation in a large cohort of healthy individuals
    Andrea J Savio
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada
    PLoS ONE 7:e51531. 2012
    ..These results indicate that CpG island shore methylation in PBCs may be influenced by genotype as well as the normal aging process...
  9. pmc Discovery of novel hypermethylated genes in prostate cancer using genomic CpG island microarrays
    Ken Kron
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    PLoS ONE 4:e4830. 2009
    ..Such occurrences often lead to the silencing of these key genes and thus they may contribute to the development of cancer, including prostate cancer...
  10. pmc Specific variants in the MLH1 gene region may drive DNA methylation, loss of protein expression, and MSI-H colorectal cancer
    Miralem Mrkonjic
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada
    PLoS ONE 5:e13314. 2010
    ..We hypothesized that specific polymorphisms in the MLH1 gene region predispose it to DNA methylation, resulting in the loss of MLH1 gene expression, mismatch-repair function, and consequently to genome-wide microsatellite instability...
  11. pmc Association of apolipoprotein E polymorphisms and dietary factors in colorectal cancer
    M Mrkonjic
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada M5G 1L5
    Br J Cancer 100:1966-74. 2009
    ..62, 95% CI 1.20-17.77) when compared with the controls. ApoE isoforms modulate the risk of MSI-H and MSS/L CRCs among high red meat consumers...
  12. pmc Promoter methylation of Wnt5a is associated with microsatellite instability and BRAF V600E mutation in two large populations of colorectal cancer patients
    J B Rawson
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada M5S 1A1
    Br J Cancer 104:1906-12. 2011
    ..Consequently, we investigated the frequency and prognostic significance of Wnt5a methylation in a large cohort of MSI-stratified CRCs...
  13. pmc The association of tumor microsatellite instability phenotype with family history of colorectal cancer
    Bharati Bapat
    Department of Pathology and Lab Medicine, Mount Sinai Hospital, and Samuel Lunenfeld Research Institute, University of Toronto, 60 Murray Street, Box 30, Toronto, M5T 3L9, Ontario, Canada
    Cancer Epidemiol Biomarkers Prev 18:967-75. 2009
    ..Familial risk associated with MSI-High tumors is primarily driven by the Amsterdam-criteria patients. MSI-Low tumors may represent a distinct subtype of colorectal cancer with respect to certain epidemiologic variables studied here...
  14. ncbi request reprint MLH1 -93G>A promoter polymorphism and the risk of microsatellite-unstable colorectal cancer
    Stavroula Raptis
    Department of Pathology, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada M5T 3L9
    J Natl Cancer Inst 99:463-74. 2007
    ..We investigated whether polymorphisms in DNA mismatch repair genes are associated with the risk of colorectal cancer...
  15. doi request reprint Association of tissue promoter methylation levels of APC, TGFβ2, HOXD3 and RASSF1A with prostate cancer progression
    Liyang Liu
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada
    Int J Cancer 129:2454-62. 2011
    ..017). Our study demonstrated that quantitative increase in promoter methylation levels of APC, HOXD3 and TGFβ2 are associated with PCa progression...
  16. doi request reprint Patients with Lynch syndrome mismatch repair gene mutations are at higher risk for not only upper tract urothelial cancer but also bladder cancer
    Sean C Skeldon
    Department of Surgical Oncology, Urology, Princess Margaret Hospital, University Health Network, Toronto, Ontario, Canada
    Eur Urol 63:379-85. 2013
    ..An increased risk for upper tract urothelial carcinoma (UTUC) has been described in this population; however, data regarding the risk for bladder cancer (BCa) are sparse...
  17. doi request reprint Vitamin D intake is negatively associated with promoter methylation of the Wnt antagonist gene DKK1 in a large group of colorectal cancer patients
    James B Rawson
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada
    Nutr Cancer 64:919-28. 2012
    ..001) and a similar trend was observed in Ontario. These results suggest that vitamin D and other dietary/lifestyle factors may alter CRC risk by mediating extracellular Wnt inhibition...
  18. ncbi request reprint MSH2 118T>C and MSH6 159C>T promoter polymorphisms and the risk of colorectal cancer
    Miralem Mrkonjic
    Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, Ontario M5T 3L9, Canada
    Carcinogenesis 28:2575-80. 2007
    ..003 and P = 0.0001, respectively). The MSH2 -118T>C polymorphism was associated with strong family history of CRC in Ontario patients...
  19. pmc Promoter methylation of Wnt antagonists DKK1 and SFRP1 is associated with opposing tumor subtypes in two large populations of colorectal cancer patients
    James B Rawson
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada
    Carcinogenesis 32:741-7. 2011
    ..This is the first large study to identify associations between Wnt antagonist promoter hypermethylation and CRC MSI subtype. These events provide insight into subtype-specific epigenetic mediation of Wnt signaling in CRC...
  20. ncbi request reprint A novel complex mutation in MSH2 contributes to both Muir-Torre and Lynch Syndrome
    Sheron Perera
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada
    J Hum Genet 55:37-41. 2010
    ....
  21. doi request reprint Functional effects of the MLH1-93G>A polymorphism on MLH1/EPM2AIP1 promoter activity
    Sheron Perera
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 1 King s College Circle, Toronto, ON, Canada
    Oncol Rep 25:809-15. 2011
    ..004) cells. The EMSA results suggest that this polymorphism alters the affinity of nuclear factors that bind to this region. Our findings indicate that the -93G>A polymorphism modifies the efficiency of MLH1/EPM2AIP1 transcription...
  22. doi request reprint Prognostic value of molecular markers, sub-stage and European Organisation for the Research and Treatment of Cancer risk scores in primary T1 bladder cancer
    Bas W G van Rhijn
    Division of Urology, University Health Network, Princess Margaret Hospital, Toronto, ON, Canada
    BJU Int 110:1169-76. 2012
    ..Future studies may lead to the incorporation of sub-stage (T1m/T1e) in the TNM classification system for urinary BC to guide clinical decision-making in T1 BC...
  23. doi request reprint Altered DNA methylation landscapes of polycomb-repressed loci are associated with prostate cancer progression and ERG oncogene expression in prostate cancer
    Ken Kron
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Clin Cancer Res 19:3450-61. 2013
    ....
  24. pmc Quantitative DNA methylation analysis of genes coding for kallikrein-related peptidases 6 and 10 as biomarkers for prostate cancer
    Ekaterina Olkhov-Mitsel
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada
    Epigenetics 7:1037-45. 2012
    ..A similar trend for KLK6 DNA methylation was observed. The results suggest that KLK6 and KLK10 DNA methylation distinguishes organ confined from locally invasive PCa and may have prognostic value...
  25. doi request reprint Correlation of ERG expression and DNA methylation biomarkers with adverse clinicopathologic features of prostate cancer
    Ken Kron
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Clin Cancer Res 18:2896-904. 2012
    ..The aim of this study was to analyze the relationship between ERG expression, DNA methylation of three biomarkers, and clinicopathologic features of prostate cancer...
  26. ncbi request reprint Apoptotic and growth regulatory genes as mutational targets in mismatch repair deficient endometrioid adenocarcinomas of young patients
    Vessela Vassileva
    Samuel Lunenfeld Research Institute, Toronto, Canada
    Oncol Rep 11:931-7. 2004
    ..06) for the overall mutation status for both pathways combined. Our results suggest that genes implicated in apoptosis may serve as targets in the progression of MSI+ EC in young patients...
  27. ncbi request reprint Comparison of clinical schemas and morphologic features in predicting Lynch syndrome in mutation-positive patients with endometrial cancer encountered in the context of familial gastrointestinal cancer registries
    Paul Ryan
    Department of Pathology, Mount Sinai Hospital, Toronto, Ontario, Canada
    Cancer 118:681-8. 2012
    ..In this study, the authors determined the efficacy of family/personal history and tumor morphology in predicting LS in a cohort of patients with EC who had mutation-proven LS...
  28. doi request reprint Evaluation and prognostic significance of ACAT1 as a marker of prostate cancer progression
    Punit Saraon
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada
    Prostate 74:372-80. 2014
    ..Previously, we identified enzymes of the ketogenic pathway, particularly ACAT1, to be elevated in aggressive prostate cancer...
  29. pmc Rac1 GTPase and the Rac1 exchange factor Tiam1 associate with Wnt-responsive promoters to enhance beta-catenin/TCF-dependent transcription in colorectal cancer cells
    Pinella Buongiorno
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, M5T 3L9, Canada
    Mol Cancer 7:73. 2008
    ..We hypothesised that Rac1 plays a direct role in enhancing the transcription of endogenous Wnt target genes by modulating the beta-catenin/TCF transcription factor complex...
  30. doi request reprint DNA methylation of HOXD3 as a marker of prostate cancer progression
    Ken J Kron
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada
    Lab Invest 90:1060-7. 2010
    ..028). The results indicate that HOXD3 methylation distinguishes low-grade prostate cancers from intermediate and high-grade ones and may also have prognostic value when considered together with pathological stage...
  31. ncbi request reprint Activation of tumor-specific splice variant Rac1b by dishevelled promotes canonical Wnt signaling and decreased adhesion of colorectal cancer cells
    Susmita Esufali
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Cancer Res 67:2469-79. 2007
    ....
  32. ncbi request reprint Does early colectomy increase desmoid risk in familial adenomatous polyposis?
    Carol Durno
    Familial Gastrointestinal Cancer Registry and Department of Surgery, Mount Sinai Hospital, University of Toronto, Toronto, Ontario, Canada
    Clin Gastroenterol Hepatol 5:1190-4. 2007
    ..We hypothesized that prophylactic colectomy at an early age might increase the risk of developing desmoids. The aim of this study was to determine whether colectomy earlier in life is a risk factor for the development of desmoid tumors...
  33. ncbi request reprint Genes involved in DNA repair are mutational targets in endometrial cancers with microsatellite instability
    Vessela Vassileva
    Samuel Lunenfeld Research Institute, Toronto, Ontario, M5G 1X5 Canada
    Cancer Res 62:4095-9. 2002
    ....
  34. doi request reprint Epigenetic biomarkers in colorectal cancer diagnostics
    James B Rawson
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, L6 304B, Box 30, Toronto, ON M5G 1X5, Canada
    Expert Rev Mol Diagn 12:499-509. 2012
    ..This review will aim to summarize the established and emerging roles of epigenetic modifications in CRC detection, prognostication and prediction of treatment response...
  35. pmc A novel and rapid method of determining the effect of unclassified MLH1 genetic variants on differential allelic expression
    Sheron Perera
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada
    J Mol Diagn 12:757-64. 2010
    ....
  36. ncbi request reprint Methylation of adenomatous polyposis coli in endometrial cancer occurs more frequently in tumors with microsatellite instability phenotype
    Michele Zysman
    Samuel Lunenfeld Research Institute, Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, Ontario, M5G 1X5, Canada
    Cancer Res 62:3663-6. 2002
    ..Our results demonstrate APC methylation in endometrial cancer for the first time and show that APC hypermethylation occurs at an increased frequency in MSI+ endometrial tumors (P = 0.01)...
  37. doi request reprint The MLH1 variants p.Arg265Cys and p.Lys618Ala affect protein stability while p.Leu749Gln affects heterodimer formation
    Sheron Perera
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada
    Hum Mutat 29:332. 2008
    ..793C>T) and p.Lys618Ala (c.1852_1853AA>GC) significantly decrease the stability of the protein, while another missense variant p.Leu749Gln (c.2246T>A) is able to compromise heterodimerization of the MLH1-PMS2 complex...
  38. ncbi request reprint Hereditary nonpolyposis colorectal cancer--molecular basis
    Christine Brezden-Masley
    Department of Surgery, Centre for Cancer Genetics, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, University of Toronto, Toronto, Ontario, Canada
    Surgery 134:29-33. 2003
  39. ncbi request reprint MYH mutations are rare in prostate cancer
    Eyun Jung Shin
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada
    J Cancer Res Clin Oncol 133:373-8. 2007
    ..A compromised base-excision repair function due to defective MYH may contribute to prostate carcinogenesis. Here, we examine the genetic contribution of MYH to prostate cancer risk...
  40. ncbi request reprint Cyclooxygenase-2 knockdown by RNA interference in colon cancer
    George S Charames
    Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, ON, Canada
    Int J Oncol 28:543-9. 2006
    ....
  41. ncbi request reprint Genomic instability and cancer
    George S Charames
    Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, Canada
    Curr Mol Med 3:589-96. 2003
    ..This review examines various molecular mechanisms of genomic instability and their relevance to cancer...
  42. pmc Considerations when analyzing the methylation status of PTEN tumor suppressor gene
    Michele A Zysman
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Am J Pathol 160:795-800. 2002
    ..Without careful consideration of the critical nucleotide differences between the two sequences, results obtained from PTEN analysis may not necessarily represent the methylation status of PTEN...
  43. ncbi request reprint An association between the 4G polymorphism in the PAI-1 promoter and the development of aggressive fibromatosis (desmoid tumor) in familial adenomatous polyposis patients
    Catherine F Li
    Program in Developmental Biology, The Hospital for Sick Children, University of Toronto, Ontario, Canada
    Fam Cancer 6:89-95. 2007
    ..This data provides additional evidence supporting an important role for PAI-1 in the pathogenesis of aggressive fibromatosis...
  44. ncbi request reprint Epigenetic regulation of kallikrein-related peptidases: there is a whole new world out there
    Maria D Pasic
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Ontario, Canada
    Biol Chem 393:319-30. 2012
    ..Experimental validation remains an essential step to confirm the KLK-miRNA interaction. Epigenetic regulation of KLKs holds promise for an array of therapeutic applications in many diseases including cancer...
  45. doi request reprint The FGFR3 mutation is related to favorable pT1 bladder cancer
    Bas W G van Rhijn
    Division of Urology, Princess Margaret Hospital, Toronto, Ontario, Canada
    J Urol 187:310-4. 2012
    ..We determined the frequency of FGFR3 mutations and P53 alterations in patients with pT1 bladder cancer and correlated these data to histopathological variables and clinical outcomes...
  46. doi request reprint Systemic Erdheim-Chester disease
    Brendan Craig Dickson
    Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, ON, Canada
    Virchows Arch 452:221-7. 2008
    ..Moreover, in confirming clonality, our observations lend additional evidence to the view that Erdheim-Chester disease represents a neoplastic process...
  47. ncbi request reprint Rho GTPases and cancer
    Pinella Buongiorno
    Samuel Lunenfeld Research Institute, 9th Floor, Room 992B, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, M5G 1X5 Canada
    Prog Mol Subcell Biol 40:29-53. 2005