- Caspase-3-independent apoptotic pathways contribute to interleukin-32γ-mediated control of Mycobacterium tuberculosis infection in THP-1 cells
Department of Medicine, Denver Veterans Affairs Medical Center, Denver, CO, USA
BMC Microbiol 15:366. 2015
..Herein, we assessed the potential roles of cathepsin-mediated apoptosis, caspase-1-mediated pyroptosis, and apoptosis-inducing factor (AIF) in mediating IL-32γ control of MTB infection in THP-1 cells...
- Inhibition of nuclear factor-kappa B activation decreases survival of Mycobacterium tuberculosis in human macrophages
Department of Medicine, Denver Veterans Affairs Medical Center, Denver, Colorado, United States of America
PLoS ONE 8:e61925. 2013
..We conclude that NFκB inhibition decreases viability of intracellular MTB in human macrophages via induction of apoptosis and autophagy...
- IL-32 expression in the airway epithelial cells of patients with Mycobacterium avium complex lung disease
Department of Medicine, Denver Veterans Affairs Medical Center, Denver, CO 80220, USA
Int Immunol 23:679-91. 2011
..These findings indicate that IL-32 facilitates host defense against MAC organisms but may also contribute to the airway inflammation associated with MAC pulmonary disease...
- Mycobacterium tuberculosis increases IP-10 and MIG protein despite inhibition of IP-10 and MIG transcription
Denver Veterans Affair Medical Center, University of Colorado Denver, Aurora, CO, United States
Tuberculosis (Edinb) 91:26-35. 2011
..The synergy between MTB and IFNγ in the induction of IP-10 and MIG protein appears to involve novel post-transcriptional events that incorporates non-canonical functions of NFκB and p38(mapk)...
- Identification and characterization of a novel testis-specific gene CKT2, which encodes a substrate for protein kinase CK2
McLaughlin Research Institute for Biomedical Science, Great Falls, MT 59405, USA
Nucleic Acids Res 37:2699-711. 2009
..Findings reported herein demonstrate that CKT2 is a target protein of native CK2alpha' in testes and suggest that CKT2 plays a role in chromatin regulation of male germ cells...
- IL-32 is a host protective cytokine against Mycobacterium tuberculosis in differentiated THP-1 human macrophages
Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado Denver at Anschutz Medical Center, Denver, CO 80045, USA
J Immunol 184:3830-40. 2010
..tuberculosis bacteria, which was modestly but significantly abrogated with a caspase-3 inhibitor. We conclude that IL-32 plays a host defense role against M. tuberculosis in differentiated THP-1 human macrophages...
- Cigarette smoke increases susceptibility to tuberculosis--evidence from in vivo and in vitro models
Mycobacteria Research Laboratories, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO, USA
J Infect Dis 203:1240-8. 2011
..Cigarette smoke (CS) exposure is an epidemiological risk factor for tuberculosis, although the biological basis has not been elucidated...
- Patients with non-tuberculous mycobacterial lung disease have elevated transforming growth factor-beta following ex vivo stimulation of blood with live Mycobacterium intracellulare
Alida R Ovrutsky
Denver Veterans Affairs Medical Center, Colorado, USA
Scand J Infect Dis 45:711-4. 2013
- Suppression of IFNgamma+mycobacterial lipoarabinomannan-induced NO by IL-4 is due to decreased IRF-1 expression
Kristin R Morris
Department of Medicine, University of Colorado Health Sciences Center, Denver, CO, USA
Tuberculosis (Edinb) 89:294-303. 2009
- Alpha-1-antitrypsin (AAT) anomalies are associated with lung disease due to rapidly growing mycobacteria and AAT inhibits Mycobacterium abscessus infection of macrophages
Edward D Chan
Department of Medicine, National Jewish Medical and Research Center, Denver, CO, USA
Scand J Infect Dis 39:690-6. 2007
..abscessus infection of monocyte-derived macrophages by up to 65% (p<0.01). AAT may be an anti-RGM host-defense factor, and anomalous AAT phenotypes or AAT deficiency may constitute risk factors for pulmonary disease due to RGM...
- Patients with nontuberculous mycobacterial lung disease exhibit unique body and immune phenotypes
Division of Infectious Diseases, University of Colorado Denver Anschutz Medical Campus, Aurora, CO, USA
Am J Respir Crit Care Med 187:197-205. 2013
..We hypothesize that unidentified factors predispose these individuals to pulmonary nontuberculous mycobacterial disease...
- Host immune response to rapidly growing mycobacteria, an emerging cause of chronic lung disease
Edward D Chan
Division of Mycobacterial and Respiratory Infections, National Jewish Health, Denver, CO 80206, USA
Am J Respir Cell Mol Biol 43:387-93. 2010
..However, much remains to be discovered on why seemingly healthy individuals, particularly slender postmenopausal women with thoracic cage anomalies, appear to be at increased risk...
- An oxidative environment promotes growth of Mycobacterium abscessus
Rebecca E Oberley-Deegan
Department of Medicine, National Jewish Health, Denver, CO 80206, USA
Free Radic Biol Med 49:1666-73. 2010
..abscessus growth is inhibited. These results lead us to postulate that antioxidants may aid in the treatment of M. abscessus infections...
- The protein of a new gene, Tctex4, interacts with protein kinase CK2beta subunit and is highly expressed in mouse testis
McLaughlin Research Institute for Biomedical Sciences, Great Falls, MT 59405, USA
Biochem Biophys Res Commun 307:86-91. 2003
..CK2beta interacts specifically with Tctex4 both in a yeast two-hybrid system and in an in vitro interaction assay. Northern blot and in situ hybridization showed that Tctex4 is a novel gene that is expressed in mouse testis...
- A surface with a biomimetic micropattern reduces colonization of Mycobacterium abscessus
Department of Medicine and Academic Affairs, National Jewish Health, Denver, CO, USA
FEMS Microbiol Lett 360:17-22. 2014
..In summary, a micropattern surface reduces the colonization of a pathogenic NTM. It remains to be seen whether this micropattern can reduce infections in humans. ..
- Tobacco exposure and susceptibility to tuberculosis: is there a smoking gun?
Edward D Chan
Department of Medicine, Denver Veterans Affairs Medical Center, 1055 Clermont St, Denver, CO 80220, USA Departments of Medicine and Academic Affairs, D509, Neustadt Building, National Jewish Health, 1400 Jackson St, Denver, CO 80206, USA Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado Anschutz Medical Campus, Research 2, Box C 272, 9th Floor, 12700 East 19th Avenue, Aurora, CO 80045, USA Electronic address
Tuberculosis (Edinb) 94:544-50. 2014
- Morphometric analysis of Th(1) and Th(2) cytokine expression in human pulmonary tuberculosis
Department of Medicine, University of Colorado School of Medicine, Denver, CO 80206, USA
Tuberculosis (Edinb) 84:375-85. 2004
..In particular, the balance between Th(1) and Th(2) cytokine responses influences the expression of disease in individuals with pulmonary TB...
- Cooccurrence of free-living amoebae and nontuberculous Mycobacteria in hospital water networks, and preferential growth of Mycobacterium avium in Acanthamoeba lenticulata
Alida R Ovrutsky
National Jewish Health, Denver, CO, USA
Appl Environ Microbiol 79:3185-92. 2013
..avium in drinking water networks and the epidemiology of disease due to this species...
- Induction of IL-8 by Mycoplasma pneumoniae membrane in BEAS-2B cells
Department of Medicin, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
Am J Physiol Lung Cell Mol Physiol 295:L220-30. 2008
..pneumoniae are also known to have anti-inflammatory effects. Whereas clarithromycin, azithromycin, and moxifloxacin individually were able to inhibit TNF-alpha-induction of IL-8, each failed to inhibit MMF-induction of IL-8...