Central glucagon-like peptide 1 receptor-induced anorexia requires glucose metabolism-mediated suppression of AMPK and is impaired by central fructoseMelissa A Burmeister
Sanford Burnham Medical Research Institute at Lake Nona, Diabetes and Obesity Research Center, Metabolic Signaling and Disease Program, 6400 Sanger Rd, Orlando, FL 32827
Am J Physiol Endocrinol Metab 304:E677-85. 2013
..We also suggest that fructose stimulates food intake by impairing central GLP-1R action. This has significant implications given the correlation between sugar consumption and obesity...
Regulation of glucose kinetics during exercise by the glucagon-like peptide-1 receptorM A Burmeister
Metabolic Signaling and Disease Program, Diabetes and Obesity Research Center, Sanford Burnham Medical Research Institute at Lake Nona, 6400 Sanger Road, Orlando, FL 32827, USA
J Physiol 590:5245-55. 2012
..These results suggest an essential role for basal Glp1r signalling in the suppression of alpha cell secretion during exercise...
- Standard operating procedures for describing and performing metabolic tests of glucose homeostasis in mice
Julio E Ayala
Vanderbilt NIH Mouse Metabolic Phenotyping Center, Nashville, TN 37232, USA
Dis Model Mech 3:525-34. 2010
..We also propose guidelines for the description of methods, presentation of data and interpretation of results. The recommendations presented in this article are based on the experience of the MMPC Consortium and other investigators...
- Glucagon-like peptide-1 receptor knockout mice are protected from high-fat diet-induced insulin resistance
Julio E Ayala
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
Endocrinology 151:4678-87. 2010
..These results show that mice lacking the Glp1r are protected from HF diet-induced muscle and hepatic insulin resistance independent of effects on total fat mass...
- The glucagon-like peptide-1 receptor regulates endogenous glucose production and muscle glucose uptake independent of its incretin action
Julio E Ayala
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
Endocrinology 150:1155-64. 2009
..In conclusion, these results show that the endogenous Glp1r regulates hepatic and muscle glucose flux independent of its ability to enhance insulin secretion...
- Endothelial nitric oxide synthase is central to skeletal muscle metabolic regulation and enzymatic signaling during exercise in vivo
Robert S Lee-Young
Department of Molecular Physiology and Biophysics, Vanderbilt University, School of Medicine, 2200 Pierce Ave, Nashville, TN 37232, U S A
Am J Physiol Regul Integr Comp Physiol 298:R1399-408. 2010
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Insulin action in the double incretin receptor knockout mouseJulio E Ayala
Vanderbilt University Medical Center, 2200 Pierce Ave, 702 Light Hall, Nashville, TN 37232, USA
Diabetes 57:288-97. 2008
..We used the hyperinsulinemic-euglycemic clamp to determine sites where insulin action may be modulated in double incretin receptor knockout (DIRKO) mice, which lack endogenous incretin action...
- Diet-induced muscle insulin resistance is associated with extracellular matrix remodeling and interaction with integrin alpha2beta1 in mice
Li Kang
Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA
Diabetes 60:416-26. 2011
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- Glucose kinetics and exercise tolerance in mice lacking the GLUT4 glucose transporter
Patrick T Fueger
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
J Physiol 582:801-12. 2007
..Finally, studies in GLUT4(-/-)HK(Tg) show that HK II improves exercise tolerance, independent of its effects on MGU...
- Glucose metabolism in vivo in four commonly used inbred mouse strains
Eric D Berglund
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
Diabetes 57:1790-9. 2008
..To characterize differences in whole-body glucose metabolism between commonly used inbred mouse strains...
- The physiological regulation of glucose flux into muscle in vivo
David H Wasserman
Department of Molecular Physiology and Biophysics and the Mouse Metabolic Phenotyping Center, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
J Exp Biol 214:254-62. 2011
..More recent research definitively shows that the distributed control paradigm more accurately defines the regulation of muscle glucose uptake as each of the three steps that define this process are important sites of flux control...
- NIH experiment in centralized mouse phenotyping: the Vanderbilt experience and recommendations for evaluating glucose homeostasis in the mouse
Owen P McGuinness
Vanderbilt NIH Mouse Metabolic Phenotyping Center, Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232 0615, USA
Am J Physiol Endocrinol Metab 297:E849-55. 2009
..To facilitate and maximize the exchange of scientific information, we suggest that guidelines be developed for methods used to assess glucose tolerance and insulin action in vivo...
- Chronic treatment with sildenafil improves energy balance and insulin action in high fat-fed conscious mice
Julio E Ayala
Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, 2200 Pierce Ave, 702 Light Hall, Nashville, TN 37232, USA
Diabetes 56:1025-33. 2007
..These results show that phosphodiesterase-5 is a potential target for therapies aimed at preventing diet-induced energy imbalance and insulin resistance...
- Considerations in the design of hyperinsulinemic-euglycemic clamps in the conscious mouse
Julio E Ayala
Vanderbilt NIDDK National Institutes of Diabetes and Digestive and Kidney Diseases Mouse Metabolic Phenotyping Center, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
Diabetes 55:390-7. 2006
..kg(-1) . min(-1)), but endoR(a) was fully suppressed with doses higher than 0.8 mU . kg(-1) . min(-1). Thus, common variations in experimental factors yield different results and should be considered in designing and interpreting clamps...
- Skeletal muscle AMP-activated protein kinase is essential for the metabolic response to exercise in vivo
Robert S Lee-Young
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
J Biol Chem 284:23925-34. 2009
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- Acute activation of central GLP-1 receptors enhances hepatic insulin action and insulin secretion in high-fat-fed, insulin resistant mice
Melissa A Burmeister
Metabolic Signaling and Disease Program, Diabetes and Obesity Research Center, Orlando, Florida, USA
Am J Physiol Endocrinol Metab 302:E334-43. 2012
..Contrasting this, activation of the central GLP-1R improves glucose homeostasis in HF-fed mice by increasing insulin levels and enhancing hepatic insulin action...
- Exendin-4 attenuates high glucose-induced cardiomyocyte apoptosis via inhibition of endoplasmic reticulum stress and activation of SERCA2a
Craig W Younce
Metabolic Signaling and Disease Program, Diabetes and Obesity Research Center, Sanford Burnham Medical Research Institute at Lake Nona, 6400 Sanger Rd, Orlando, FL 32837, USA
Am J Physiol Cell Physiol 304:C508-18. 2013
..These findings identify a novel mechanism whereby Glp1-based therapies could be used as treatments for diabetic cardiomyopathy...
- Regulation of endogenous glucose production in glucose transporter 4 over-expressing mice
Eric D Berglund
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
PLoS ONE 7:e52355. 2012
..These data emphasize that anti-diabetic therapies that target skeletal muscle, heart, and/or adipose tissue likely positively impact the liver...
- Interaction of physiological mechanisms in control of muscle glucose uptake
David H Wasserman
Department of Molecular Physiological and Biophysics, Mouse Metabolic Phenotyping Center, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
Clin Exp Pharmacol Physiol 32:319-23. 2005
..4. There is a growing body of data that suggests that insulin resistance to muscle glucose uptake can be because of impairments in any one or more of the three steps that comprise the process...
- The nuclear receptor PPARβ/δ programs muscle glucose metabolism in cooperation with AMPK and MEF2
Zhenji Gan
Diabetes and Obesity Research Center, Sanford Burnham Medical Research Institute, Orlando, Florida 32827, USA
Genes Dev 25:2619-30. 2011
..These results identify a transcriptional regulatory mechanism that increases capacity for muscle glucose utilization in a pattern that resembles the effects of exercise training...
- Accessory elements, flanking DNA sequence, and promoter context play key roles in determining the efficacy of insulin and phorbol ester signaling through the malic enzyme and collagenase-1 AP-1 motifs
Julio E Ayala
Department of Molecular Physiology and Biophysics, Vanderbilt University Medical School, Nashville, Tennessee 37232, USA
J Biol Chem 277:27935-44. 2002
..But even in the context of the collagenase-1 promoter, the effects of both insulin and phorbol esters, mediated through the ME AP-1 motif are dependent on accessory factors...
