Research Topics
| Bernhard SeibothSummaryAffiliation: Vienna University of Technology Country: Austria Publications
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Detail Information
Publications
A homologous production system for Trichoderma reesei secreted proteins in a cellulase-free backgroundFatma Uzbas
Biological Sciences and Bioengineering, Sabanci University, Orhanli, Tuzla, Istanbul, Turkey
Appl Microbiol Biotechnol 93:1601-8. 2012..Hence this system can be used as a vehicle for rapid and high-throughput testing of cellulase muteins in a homologous background...- Fungal arabinan and L-arabinose metabolismBernhard Seiboth
Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Wien, Austria
Appl Microbiol Biotechnol 89:1665-73. 2011..Further genetic and biochemical analysis provided evidence that L-arabinose and the interconnected D-xylose pathway are also involved in the oxidoreductive degradation of the hexose D-galactose... - The Hypocrea jecorina (Trichoderma reesei) hypercellulolytic mutant RUT C30 lacks a 85 kb (29 gene-encoding) region of the wild-type genomeVerena Seidl
Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Getreidemarkt 9 166 5, A 1060 Wien, Austria
BMC Genomics 9:327. 2008..To date this and an additional frame-shift mutation in the glycoprotein-processing beta-glucosidase II encoding gene are the only known genetic differences in strain RUT C30... 
The D-xylose reductase of Hypocrea jecorina is the major aldose reductase in pentose and D-galactose catabolism and necessary for beta-galactosidase and cellulase induction by lactoseBernhard Seiboth
Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Austria
Mol Microbiol 66:890-900. 2007..Our results show that the role of the H. jecorina XYL1 is not restricted to D-xylose catabolism and demonstrates its importance for induction of cellulases and beta-galactosidases...- A complete survey of Trichoderma chitinases reveals three distinct subgroups of family 18 chitinasesVerena Seidl
Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, TU Vienna, Austria
FEBS J 272:5923-39. 2005..solani. Therefore, group C and the novel clade in group B may contain chitinases of potential relevance for the biocontrol properties of Trichoderma... - Induction of the gal pathway and cellulase genes involves no transcriptional inducer function of the galactokinase in Hypocrea jecorinaLukas Hartl
Molecular Biotechnology Group, Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Technische Universitat Wien, Getreidemarkt 9 166 5, A 1060 Vienna, Austria
J Biol Chem 282:18654-9. 2007.... - The fungal STRE-element-binding protein Seb1 is involved but not essential for glycerol dehydrogenase (gld1) gene expression and glycerol accumulation in Trichoderma atroviride during osmotic stressVerena Seidl
Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Getreidemarkt 9 166 5, A 1060 Wien, Austria
Fungal Genet Biol 41:1132-40. 2004..The data suggest that Seb1 is only involved in, but not essential for osmotic stress response which is in contrast to the yeast orthologues Msn2/4... - The CRE1 carbon catabolite repressor of the fungus Trichoderma reesei: a master regulator of carbon assimilationThomas Portnoy
Research Area Gene Technology and Applied Biochemistry, Institute of ChemicalEngineering, Technische Universitat Wien, Getreidemarkt, Vienna, Austria
BMC Genomics 12:269. 2011..In lower multicellular fungi, the C2H2 zinc finger CreA/CRE1 protein has been shown to act as the transcriptional repressor in this process. However, the complete list of its gene targets is not known... - Role of the bga1-encoded extracellular {beta}-galactosidase of Hypocrea jecorina in cellulase induction by lactoseBernhard Seiboth
Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, TU Vienna, Getreidemarkt 9 1665, A 1060 Vienna, Austria
Appl Environ Microbiol 71:851-7. 2005..The data indicate that bga1-encoded beta-galactosidase activity is a critical factor for cellulase production on lactose... - Characterization of the bga1-encoded glycoside hydrolase family 35 beta-galactosidase of Hypocrea jecorina with galacto-beta-D-galactanase activityChristian Gamauf
Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Austria
FEBS J 274:1691-700. 2007..beta-Galactosidase is inhibited by its reaction product D-galactose and the enzyme also shows a significant transferase activity which results in the formation of galacto-oligosaccharides... 
Gene targeting in a nonhomologous end joining deficient Hypocrea jecorinaZhang Guangtao
Molecular Biotechnology, Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, TU Wien, Getreidemarkt 9, A 1060 Wien, Austria
J Biotechnol 139:146-51. 2009..Together with the recently annotated genomic sequence of H. jecorina, this system provides a useful tool for a genome-wide functional gene analysis on a high-throughput scale to improve the biotechnological potential of this fungus...- The metabolic role and evolution of L-arabinitol 4-dehydrogenase of Hypocrea jecorinaManuela Pail
Division of Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, TU Wien, Vienna, Austria
Eur J Biochem 271:1864-72. 2004.... - The galactokinase of Hypocrea jecorina is essential for cellulase induction by lactose but dispensable for growth on d-galactoseBernhard Seiboth
Division of Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, TU Wein, Vienna, Austria
Mol Microbiol 51:1015-25. 2004.... - Antagonism of Pythium blight of zucchini by Hypocrea jecorina does not require cellulase gene expression but is improved by carbon catabolite derepressionVerena Seidl
Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, TU Vienna, Vienna, Austria
FEMS Microbiol Lett 257:145-51. 2006..ultimum as strain QM9414 did. We conclude that cellulase formation is dispensable for biocontrol of P. ultimum, whereas carbon catabolite derepression increases the antagonistic ability by apparently acting on other target genes... - Expression of biomass-degrading enzymes is a major event during conidium development in Trichoderma reeseiBenjamin Metz
Institute of Chemical Engineering, University of Technology of Vienna, Vienna, Austria
Eukaryot Cell 10:1527-35. 2011..reesei, contributes to the hypothesis that the ability to hydrolyze plant biomass is a major trait of this fungus enabling it to break dormancy and reinitiate vegetative growth after a period of facing unfavorable conditions... - Sequential gene deletions in Hypocrea jecorina using a single blaster cassetteLukas Hartl
Molecular Biotechnology, Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, TU Wien, Getreidemarkt 9-166.5, 1060 Wien, Austria
Curr Genet 48:204-11. 2005..The double Deltaglk1Deltahxk1 deletion strain was completely unable to grow on either d-glucose or d-fructose... - Molecular regulation of arabinan and L-arabinose metabolism in Hypocrea jecorina (Trichoderma reesei)Eda Akel
Molecular Biotechnology Group, Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Getreidemarkt 9, Vienna, Austria
Eukaryot Cell 8:1837-44. 2009..jecorina for growth on arabinan requires an early pathway intermediate (L-arabinitol or L-arabinose), the first enzyme of the pathway XYL1, and in the case of abf2 and bxl1 also the function of the cellulase regulator XYR1... - The putative protein methyltransferase LAE1 controls cellulase gene expression in Trichoderma reeseiBernhard Seiboth
Institute of Chemical Engineering, University of Technology of Vienna, Gumpendorferstrasse 1a, A 1060 Vienna, Austria
Mol Microbiol 84:1150-64. 2012..Our data demonstrate that the putative protein methyltransferase LAE1 is essential for cellulase gene expression in T. reesei through mechanisms that remain to be identified... - The Hypocrea jecorina gal10 (uridine 5'-diphosphate-glucose 4-epimerase-encoding) gene differs from yeast homologues in structure, genomic organization and expressionBernhard Seiboth
Mikrobielle Biochemie und Gentechnik, Institut fur Verfahrenstechnik, Umwelttechnik und Technische Biowissenschaften, TU Wien, Getreidemarkt 9, A 1060 Vienna, Austria
Gene 295:143-9. 2002..5-kilobase. gal10 is constitutively expressed, and--unlike H. jecorina gal7--not further stimulated by D-galactose or L-arabinose or its corresponding polyols... - D-xylose metabolism in Hypocrea jecorina: loss of the xylitol dehydrogenase step can be partially compensated for by lad1-encoded L-arabinitol-4-dehydrogenaseBernhard Seiboth
Abteilung für Angewandte Biochemie und Gentechnologie, Institut fur Verfahrenstechnik, Umwelttechnik, und Technische Biowissenschaften, A 1060 Wien, Austria
Eukaryot Cell 2:867-75. 2003.... - Global carbon utilization profiles of wild-type, mutant, and transformant strains of Hypocrea jecorinaIrina S Druzhinina
Research Area of Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Getreidemarkt 9 1665, A 1060 Vienna, Austria
Appl Environ Microbiol 72:2126-33. 2006.... - The Hypocrea jecorina (syn. Trichoderma reesei) lxr1 gene encodes a D-mannitol dehydrogenase and is not involved in L-arabinose catabolismBenjamin Metz
Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, TU Vienna, Getreidemarkt 9, A 1060 Wien, Austria
FEBS Lett 583:1309-13. 2009..We conclude that LXR1 is a D-mannitol 2-dehydrogenase and that a true LXR1 is still awaiting discovery... - A versatile toolkit for high throughput functional genomics with Trichoderma reeseiAndre Schuster
Research Area of Gene Technology and Applied Biochemistry, Institute for Chemical Engineering, Vienna University of Technology, Gumpendorfer Strasse 1a 1665, A 1060 Wien, Austria
Biotechnol Biofuels 5:1. 2012..abstract:.. - Metabolic Engineering of Inducer Formation for Cellulase and Hemicellulase Gene Expression in Trichoderma reeseiBernhard Seiboth
Research Division Biotechnology and Microbiology, Institute of Chemical Engineering, Vienna University of Technology, Gumpendorferstraße 1a, 166 5, A 1060, Vienna, Austria
Subcell Biochem 64:367-90. 2012..This review aims at a description of the state of art in this area... - The Snf1 kinase of the filamentous fungus Hypocrea jecorina phosphorylates regulation-relevant serine residues in the yeast carbon catabolite repressor Mig1 but not in the filamentous fungal counterpart Cre1Angela Cziferszky
Division of Applied Biochemistry and Gene Technology, Institute of Chemical Engineering, TU Wien, Getreidemarkt 9/166.5, A-1060 Wien, Austria
Fungal Genet Biol 40:166-75. 2003..We conclude that the Snf1 kinase from H. jecorina is not involved in the phosphorylation of Cre1...