Bernhard Seiboth

Summary

Affiliation: Vienna University of Technology
Country: Austria

Publications

  1. pmc A novel L-xylulose reductase essential for L-arabinose catabolism in Trichoderma reesei
    Benjamin Metz
    Research Division Biotechnology and Microbiology, Institute of Chemical Engineering, Vienna University of Technology, Vienna, Austria
    Biochemistry 52:2453-60. 2013
  2. pmc A homologous production system for Trichoderma reesei secreted proteins in a cellulase-free background
    Fatma Uzbas
    Biological Sciences and Bioengineering, Sabanci University, Orhanli, Tuzla, Istanbul, Turkey
    Appl Microbiol Biotechnol 93:1601-8. 2012
  3. pmc Fungal arabinan and L-arabinose metabolism
    Bernhard Seiboth
    Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Wien, Austria
    Appl Microbiol Biotechnol 89:1665-73. 2011
  4. pmc The Hypocrea jecorina (Trichoderma reesei) hypercellulolytic mutant RUT C30 lacks a 85 kb (29 gene-encoding) region of the wild-type genome
    Verena Seidl
    Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Getreidemarkt 9 166 5, A 1060 Wien, Austria
    BMC Genomics 9:327. 2008
  5. pmc Molecular regulation of arabinan and L-arabinose metabolism in Hypocrea jecorina (Trichoderma reesei)
    Eda Akel
    Molecular Biotechnology Group, Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Getreidemarkt 9, Vienna, Austria
    Eukaryot Cell 8:1837-44. 2009
  6. ncbi request reprint The D-xylose reductase of Hypocrea jecorina is the major aldose reductase in pentose and D-galactose catabolism and necessary for beta-galactosidase and cellulase induction by lactose
    Bernhard Seiboth
    Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Austria
    Mol Microbiol 66:890-900. 2007
  7. ncbi request reprint Induction of the gal pathway and cellulase genes involves no transcriptional inducer function of the galactokinase in Hypocrea jecorina
    Lukas Hartl
    Molecular Biotechnology Group, Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Technische Universitat Wien, Getreidemarkt 9 166 5, A 1060 Vienna, Austria
    J Biol Chem 282:18654-9. 2007
  8. ncbi request reprint A complete survey of Trichoderma chitinases reveals three distinct subgroups of family 18 chitinases
    Verena Seidl
    Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, TU Vienna, Austria
    FEBS J 272:5923-39. 2005
  9. ncbi request reprint The fungal STRE-element-binding protein Seb1 is involved but not essential for glycerol dehydrogenase (gld1) gene expression and glycerol accumulation in Trichoderma atroviride during osmotic stress
    Verena Seidl
    Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Getreidemarkt 9 166 5, A 1060 Wien, Austria
    Fungal Genet Biol 41:1132-40. 2004
  10. pmc Functional analyses of Trichoderma reesei LAE1 reveal conserved and contrasting roles of this regulator
    Razieh Karimi-Aghcheh
    Institute of Chemical Engineering, Research Division Biotechnology and Microbiology, University of Technology, 1060 Vienna, Austria
    G3 (Bethesda) 3:369-78. 2013

Collaborators

Detail Information

Publications30

  1. pmc A novel L-xylulose reductase essential for L-arabinose catabolism in Trichoderma reesei
    Benjamin Metz
    Research Division Biotechnology and Microbiology, Institute of Chemical Engineering, Vienna University of Technology, Vienna, Austria
    Biochemistry 52:2453-60. 2013
    ....
  2. pmc A homologous production system for Trichoderma reesei secreted proteins in a cellulase-free background
    Fatma Uzbas
    Biological Sciences and Bioengineering, Sabanci University, Orhanli, Tuzla, Istanbul, Turkey
    Appl Microbiol Biotechnol 93:1601-8. 2012
    ..Hence this system can be used as a vehicle for rapid and high-throughput testing of cellulase muteins in a homologous background...
  3. pmc Fungal arabinan and L-arabinose metabolism
    Bernhard Seiboth
    Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Wien, Austria
    Appl Microbiol Biotechnol 89:1665-73. 2011
    ..Further genetic and biochemical analysis provided evidence that L-arabinose and the interconnected D-xylose pathway are also involved in the oxidoreductive degradation of the hexose D-galactose...
  4. pmc The Hypocrea jecorina (Trichoderma reesei) hypercellulolytic mutant RUT C30 lacks a 85 kb (29 gene-encoding) region of the wild-type genome
    Verena Seidl
    Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Getreidemarkt 9 166 5, A 1060 Wien, Austria
    BMC Genomics 9:327. 2008
    ..To date this and an additional frame-shift mutation in the glycoprotein-processing beta-glucosidase II encoding gene are the only known genetic differences in strain RUT C30...
  5. pmc Molecular regulation of arabinan and L-arabinose metabolism in Hypocrea jecorina (Trichoderma reesei)
    Eda Akel
    Molecular Biotechnology Group, Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Getreidemarkt 9, Vienna, Austria
    Eukaryot Cell 8:1837-44. 2009
    ..jecorina for growth on arabinan requires an early pathway intermediate (L-arabinitol or L-arabinose), the first enzyme of the pathway XYL1, and in the case of abf2 and bxl1 also the function of the cellulase regulator XYR1...
  6. ncbi request reprint The D-xylose reductase of Hypocrea jecorina is the major aldose reductase in pentose and D-galactose catabolism and necessary for beta-galactosidase and cellulase induction by lactose
    Bernhard Seiboth
    Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Austria
    Mol Microbiol 66:890-900. 2007
    ..Our results show that the role of the H. jecorina XYL1 is not restricted to D-xylose catabolism and demonstrates its importance for induction of cellulases and beta-galactosidases...
  7. ncbi request reprint Induction of the gal pathway and cellulase genes involves no transcriptional inducer function of the galactokinase in Hypocrea jecorina
    Lukas Hartl
    Molecular Biotechnology Group, Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Technische Universitat Wien, Getreidemarkt 9 166 5, A 1060 Vienna, Austria
    J Biol Chem 282:18654-9. 2007
    ....
  8. ncbi request reprint A complete survey of Trichoderma chitinases reveals three distinct subgroups of family 18 chitinases
    Verena Seidl
    Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, TU Vienna, Austria
    FEBS J 272:5923-39. 2005
    ..solani. Therefore, group C and the novel clade in group B may contain chitinases of potential relevance for the biocontrol properties of Trichoderma...
  9. ncbi request reprint The fungal STRE-element-binding protein Seb1 is involved but not essential for glycerol dehydrogenase (gld1) gene expression and glycerol accumulation in Trichoderma atroviride during osmotic stress
    Verena Seidl
    Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Getreidemarkt 9 166 5, A 1060 Wien, Austria
    Fungal Genet Biol 41:1132-40. 2004
    ..The data suggest that Seb1 is only involved in, but not essential for osmotic stress response which is in contrast to the yeast orthologues Msn2/4...
  10. pmc Functional analyses of Trichoderma reesei LAE1 reveal conserved and contrasting roles of this regulator
    Razieh Karimi-Aghcheh
    Institute of Chemical Engineering, Research Division Biotechnology and Microbiology, University of Technology, 1060 Vienna, Austria
    G3 (Bethesda) 3:369-78. 2013
    ..Our data show that the biological roles of A. nidulans LaeA and T. reesei LAE1 are much less conserved than hitherto thought. In T. reesei, LAE1 appears predominantly to regulate genes increasing relative fitness in its environment...
  11. pmc The CRE1 carbon catabolite repressor of the fungus Trichoderma reesei: a master regulator of carbon assimilation
    Thomas Portnoy
    Research Area Gene Technology and Applied Biochemistry, Institute of ChemicalEngineering, Technische Universitat Wien, Getreidemarkt, Vienna, Austria
    BMC Genomics 12:269. 2011
    ..In lower multicellular fungi, the C2H2 zinc finger CreA/CRE1 protein has been shown to act as the transcriptional repressor in this process. However, the complete list of its gene targets is not known...
  12. ncbi request reprint The metabolic role and evolution of L-arabinitol 4-dehydrogenase of Hypocrea jecorina
    Manuela Pail
    Division of Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, TU Wien, Vienna, Austria
    Eur J Biochem 271:1864-72. 2004
    ....
  13. pmc Role of the bga1-encoded extracellular {beta}-galactosidase of Hypocrea jecorina in cellulase induction by lactose
    Bernhard Seiboth
    Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, TU Vienna, Getreidemarkt 9 1665, A 1060 Vienna, Austria
    Appl Environ Microbiol 71:851-7. 2005
    ..The data indicate that bga1-encoded beta-galactosidase activity is a critical factor for cellulase production on lactose...
  14. ncbi request reprint Characterization of the bga1-encoded glycoside hydrolase family 35 beta-galactosidase of Hypocrea jecorina with galacto-beta-D-galactanase activity
    Christian Gamauf
    Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Austria
    FEBS J 274:1691-700. 2007
    ..beta-Galactosidase is inhibited by its reaction product D-galactose and the enzyme also shows a significant transferase activity which results in the formation of galacto-oligosaccharides...
  15. doi request reprint Gene targeting in a nonhomologous end joining deficient Hypocrea jecorina
    Zhang Guangtao
    Molecular Biotechnology, Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, TU Wien, Getreidemarkt 9, A 1060 Wien, Austria
    J Biotechnol 139:146-51. 2009
    ..Together with the recently annotated genomic sequence of H. jecorina, this system provides a useful tool for a genome-wide functional gene analysis on a high-throughput scale to improve the biotechnological potential of this fungus...
  16. pmc Expression of biomass-degrading enzymes is a major event during conidium development in Trichoderma reesei
    Benjamin Metz
    Institute of Chemical Engineering, University of Technology of Vienna, Vienna, Austria
    Eukaryot Cell 10:1527-35. 2011
    ..reesei, contributes to the hypothesis that the ability to hydrolyze plant biomass is a major trait of this fungus enabling it to break dormancy and reinitiate vegetative growth after a period of facing unfavorable conditions...
  17. pmc The putative protein methyltransferase LAE1 controls cellulase gene expression in Trichoderma reesei
    Bernhard Seiboth
    Institute of Chemical Engineering, University of Technology of Vienna, Gumpendorferstrasse 1a, A 1060 Vienna, Austria
    Mol Microbiol 84:1150-64. 2012
    ..Our data demonstrate that the putative protein methyltransferase LAE1 is essential for cellulase gene expression in T. reesei through mechanisms that remain to be identified...
  18. pmc Systems analysis of lactose metabolism in Trichoderma reesei identifies a lactose permease that is essential for cellulase induction
    Christa Ivanova
    Institute of Chemical Engineering, University of Technology of Vienna, Vienna, Austria
    PLoS ONE 8:e62631. 2013
    ..reesei. These data shed new light on the mechanism by which T. reesei metabolizes lactose and offers strategies for its improvement. They also illuminate the key role of ß-D-galactosides in habitat specificity of this fungus...
  19. pmc Xylanase gene transcription in Trichoderma reesei is triggered by different inducers representing different hemicellulosic pentose polymers
    Silvia Herold
    Research Division Biotechnology and Microbiology, Institute of Chemical Engineering, Vienna University of Technology, Vienna, Austria
    Eukaryot Cell 12:390-8. 2013
    ..We conclude that the expression of the T. reesei xylanases can be induced by both D-xylose and L-arabinose, but independently of each other and by using different inducing metabolites...
  20. ncbi request reprint Antagonism of Pythium blight of zucchini by Hypocrea jecorina does not require cellulase gene expression but is improved by carbon catabolite derepression
    Verena Seidl
    Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, TU Vienna, Vienna, Austria
    FEMS Microbiol Lett 257:145-51. 2006
    ..ultimum as strain QM9414 did. We conclude that cellulase formation is dispensable for biocontrol of P. ultimum, whereas carbon catabolite derepression increases the antagonistic ability by apparently acting on other target genes...
  21. ncbi request reprint The galactokinase of Hypocrea jecorina is essential for cellulase induction by lactose but dispensable for growth on d-galactose
    Bernhard Seiboth
    Division of Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, TU Wein, Vienna, Austria
    Mol Microbiol 51:1015-25. 2004
    ....
  22. doi request reprint The Hypocrea jecorina (syn. Trichoderma reesei) lxr1 gene encodes a D-mannitol dehydrogenase and is not involved in L-arabinose catabolism
    Benjamin Metz
    Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, TU Vienna, Getreidemarkt 9, A 1060 Wien, Austria
    FEBS Lett 583:1309-13. 2009
    ..We conclude that LXR1 is a D-mannitol 2-dehydrogenase and that a true LXR1 is still awaiting discovery...
  23. ncbi request reprint Sequential gene deletions in Hypocrea jecorina using a single blaster cassette
    Lukas Hartl
    Molecular Biotechnology, Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, TU Wien, Getreidemarkt 9 166 5, 1060 Wien, Austria
    Curr Genet 48:204-11. 2005
    ..The double Deltaglk1Deltahxk1 deletion strain was completely unable to grow on either d-glucose or d-fructose...
  24. doi request reprint Heterologous expression of an Agaricus meleagris pyranose dehydrogenase-encoding gene in Aspergillus spp. and characterization of the recombinant enzyme
    Ines Pisanelli
    Department of Food Sciences and Technology, University of Natural Resources and Applied Life Sciences BOKU, Vienna, Austria
    Appl Microbiol Biotechnol 86:599-606. 2010
    ..We report here, for the first time, the heterologous expression of one of these genes, encoding the major PDH protein in Agaricus meleagris, in the filamentous fungi Aspergillus nidulans, and Aspergillus niger...
  25. pmc D-xylose metabolism in Hypocrea jecorina: loss of the xylitol dehydrogenase step can be partially compensated for by lad1-encoded L-arabinitol-4-dehydrogenase
    Bernhard Seiboth
    Abteilung für Angewandte Biochemie und Gentechnologie, Institut fur Verfahrenstechnik, Umwelttechnik, und Technische Biowissenschaften, A 1060 Wien, Austria
    Eukaryot Cell 2:867-75. 2003
    ....
  26. ncbi request reprint The Hypocrea jecorina gal10 (uridine 5'-diphosphate-glucose 4-epimerase-encoding) gene differs from yeast homologues in structure, genomic organization and expression
    Bernhard Seiboth
    Mikrobielle Biochemie und Gentechnik, Institut fur Verfahrenstechnik, Umwelttechnik und Technische Biowissenschaften, TU Wien, Getreidemarkt 9, A 1060 Vienna, Austria
    Gene 295:143-9. 2002
    ..5-kilobase. gal10 is constitutively expressed, and--unlike H. jecorina gal7--not further stimulated by D-galactose or L-arabinose or its corresponding polyols...
  27. pmc A versatile toolkit for high throughput functional genomics with Trichoderma reesei
    Andre Schuster
    Research Area of Gene Technology and Applied Biochemistry, Institute for Chemical Engineering, Vienna University of Technology, Gumpendorfer Strasse 1a 1665, A 1060 Wien, Austria
    Biotechnol Biofuels 5:1. 2012
    ..abstract:..
  28. pmc Global carbon utilization profiles of wild-type, mutant, and transformant strains of Hypocrea jecorina
    Irina S Druzhinina
    Research Area of Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Getreidemarkt 9 1665, A 1060 Vienna, Austria
    Appl Environ Microbiol 72:2126-33. 2006
    ....
  29. doi request reprint Metabolic Engineering of Inducer Formation for Cellulase and Hemicellulase Gene Expression in Trichoderma reesei
    Bernhard Seiboth
    Research Division Biotechnology and Microbiology, Institute of Chemical Engineering, Vienna University of Technology, Gumpendorferstraße 1a, 166 5, A 1060, Vienna, Austria
    Subcell Biochem 64:367-90. 2012
    ..This review aims at a description of the state of art in this area...
  30. ncbi request reprint The Snf1 kinase of the filamentous fungus Hypocrea jecorina phosphorylates regulation-relevant serine residues in the yeast carbon catabolite repressor Mig1 but not in the filamentous fungal counterpart Cre1
    Angela Cziferszky
    Division of Applied Biochemistry and Gene Technology, Institute of Chemical Engineering, TU Wien, Getreidemarkt 9 166 5, A 1060 Wien, Austria
    Fungal Genet Biol 40:166-75. 2003
    ..We conclude that the Snf1 kinase from H. jecorina is not involved in the phosphorylation of Cre1...