R Valenta

Summary

Affiliation: University of Vienna
Country: Austria

Publications

  1. ncbi request reprint Recombinant allergen molecules: tools to study effector cell activation
    R Valenta
    Department of Pathophysiology, Vienna General Hospital, University of Vienna, Austria
    Immunol Rev 179:119-27. 2001
  2. ncbi request reprint From allergen structure to new forms of allergen-specific immunotherapy
    Rudolf Valenta
    Department of Pathophysiology, University of Vienna Medical School, Vienna General Hospital, Waehringer Guertel 18 20, A 1090, Vienna, Austria
    Curr Opin Immunol 14:718-27. 2002
  3. ncbi request reprint Intranasal treatment with a recombinant hypoallergenic derivative of the major birch pollen allergen Bet v 1 prevents allergic sensitization and airway inflammation in mice
    U Wiedermann
    Department of Clinical Pharmacology, University of Vienna, Austria
    Int Arch Allergy Immunol 126:68-77. 2001
  4. ncbi request reprint Molecular, immunological, and structural characterization of Phl p 6, a major allergen and P-particle-associated protein from Timothy grass (Phleum pratense) pollen
    S Vrtala
    Institute of General and Experimental Pathology, Vienna General Hospital, University of Vienna, Austria
    J Immunol 163:5489-96. 1999
  5. ncbi request reprint Mucosal tolerance induction with hypoallergenic molecules in a murine model of allergic asthma
    U Wiedermann
    Department of Pathophysiology, University of Vienna, Austria
    Int Arch Allergy Immunol 124:391-4. 2001
  6. doi request reprint Carrier-bound nonallergenic Der p 2 peptides induce IgG antibodies blocking allergen-induced basophil activation in allergic patients
    K W Chen
    Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria
    Allergy 67:609-21. 2012
  7. ncbi request reprint Non-anaphylactic surface-exposed peptides of the major birch pollen allergen, Bet v 1, for preventive vaccination
    M Focke
    Department of Pathophysiology, Vienna General Hospital, Medical University of Vienna, Austria
    Clin Exp Allergy 34:1525-33. 2004
  8. pmc Purification, biochemical, and immunological characterisation of a major food allergen: different immunoglobulin E recognition of the apo- and calcium-bound forms of carp parvalbumin
    A Bugajska-Schretter
    Institute of Medical and Chemical Laboratory Diagnostics, AKH, University of Vienna, Austria
    Gut 46:661-9. 2000
  9. ncbi request reprint Nonanaphylactic synthetic peptides derived from B cell epitopes of the major grass pollen allergen, Phl p 1, for allergy vaccination
    M Focke
    Department of Pathophysiology, Vienna General Hospital, AKH, University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria
    FASEB J 15:2042-4. 2001
  10. doi request reprint Recombinant allergen-based monitoring of antibody responses during injection grass pollen immunotherapy and after 5 years of discontinuation
    E Gadermaier
    Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna General Hospital, Wahringer Gurtel 18 20, Vienna, Austria
    Allergy 66:1174-82. 2011

Detail Information

Publications128 found, 100 shown here

  1. ncbi request reprint Recombinant allergen molecules: tools to study effector cell activation
    R Valenta
    Department of Pathophysiology, Vienna General Hospital, University of Vienna, Austria
    Immunol Rev 179:119-27. 2001
    ..Such allergen derivatives may be used for allergen-specific immunotherapy with reduced risk of inducing anaphylactic side effects...
  2. ncbi request reprint From allergen structure to new forms of allergen-specific immunotherapy
    Rudolf Valenta
    Department of Pathophysiology, University of Vienna Medical School, Vienna General Hospital, Waehringer Guertel 18 20, A 1090, Vienna, Austria
    Curr Opin Immunol 14:718-27. 2002
    ....
  3. ncbi request reprint Intranasal treatment with a recombinant hypoallergenic derivative of the major birch pollen allergen Bet v 1 prevents allergic sensitization and airway inflammation in mice
    U Wiedermann
    Department of Clinical Pharmacology, University of Vienna, Austria
    Int Arch Allergy Immunol 126:68-77. 2001
    ..The major birch pollen allergen Bet v 1 represents one of the most prevalent environmental allergens responsible for allergic airway inflammation...
  4. ncbi request reprint Molecular, immunological, and structural characterization of Phl p 6, a major allergen and P-particle-associated protein from Timothy grass (Phleum pratense) pollen
    S Vrtala
    Institute of General and Experimental Pathology, Vienna General Hospital, University of Vienna, Austria
    J Immunol 163:5489-96. 1999
    ....
  5. ncbi request reprint Mucosal tolerance induction with hypoallergenic molecules in a murine model of allergic asthma
    U Wiedermann
    Department of Pathophysiology, University of Vienna, Austria
    Int Arch Allergy Immunol 124:391-4. 2001
    ..We conclude from our studies that mucosal tolerance induction with hypoallergenic molecules could provide a safe and convenient treatment strategy against type I allergies...
  6. doi request reprint Carrier-bound nonallergenic Der p 2 peptides induce IgG antibodies blocking allergen-induced basophil activation in allergic patients
    K W Chen
    Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria
    Allergy 67:609-21. 2012
    ..The aim of this study was to develop and characterize an allergy vaccine based on carrier-bound Der p 2 peptides, which should allow reducing IgE- and T-cell-mediated side-effects during specific immunotherapy (SIT)...
  7. ncbi request reprint Non-anaphylactic surface-exposed peptides of the major birch pollen allergen, Bet v 1, for preventive vaccination
    M Focke
    Department of Pathophysiology, Vienna General Hospital, Medical University of Vienna, Austria
    Clin Exp Allergy 34:1525-33. 2004
    ..Almost 100 million allergic patients are sensitized to the major birch pollen allergen, Bet v 1, a 17 kDa protein containing most of the IgE epitopes present in pollens of trees belonging to the Fagales order and plant-derived food...
  8. pmc Purification, biochemical, and immunological characterisation of a major food allergen: different immunoglobulin E recognition of the apo- and calcium-bound forms of carp parvalbumin
    A Bugajska-Schretter
    Institute of Medical and Chemical Laboratory Diagnostics, AKH, University of Vienna, Austria
    Gut 46:661-9. 2000
    ..Almost 4% of the population suffer from food allergy which is an adverse reaction to food with an underlying immunological mechanism...
  9. ncbi request reprint Nonanaphylactic synthetic peptides derived from B cell epitopes of the major grass pollen allergen, Phl p 1, for allergy vaccination
    M Focke
    Department of Pathophysiology, Vienna General Hospital, AKH, University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria
    FASEB J 15:2042-4. 2001
    ..We thus demonstrate that synthetic hypoallergenic peptides derived from B cell epitopes of major allergens represent safe vaccine candidates for the treatment of IgE- mediated allergies...
  10. doi request reprint Recombinant allergen-based monitoring of antibody responses during injection grass pollen immunotherapy and after 5 years of discontinuation
    E Gadermaier
    Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna General Hospital, Wahringer Gurtel 18 20, Vienna, Austria
    Allergy 66:1174-82. 2011
    ..Subcutaneous injection immunotherapy (SCIT) is considered as antigen-specific and disease-modifying treatment with long-lasting effect...
  11. doi request reprint Reducing allergenicity by altering allergen fold: a mosaic protein of Phl p 1 for allergy vaccination
    T Ball
    Department of Pathophysiology, Center for Physiology, Pathophysiology and Immunology, Medical University of Vienna, Austria
    Allergy 64:569-80. 2009
    ..Our objective was the construction, purification and immunologic characterization of a genetically modified derivative of the major timothy grass pollen allergen, Phl p 1 for immunotherapy of grass pollen allergy...
  12. pmc Vaccination with genetically engineered allergens prevents progression of allergic disease
    V Niederberger
    Department of Otorhinolaryngology, Vienna General Hospital, University of Vienna, 1090 Vienna, Austria
    Proc Natl Acad Sci U S A 101:14677-82. 2004
    ..Vaccination with genetically engineered allergen derivatives is a therapy for allergy that not only ameliorates allergic reactions but also reduces the IgE production underlying the disease...
  13. doi request reprint Carrier-bound Alt a 1 peptides without allergenic activity for vaccination against Alternaria alternata allergy
    T E Twaroch
    Christian Doppler Laboratory for Allergy Research, Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria
    Clin Exp Allergy 42:966-75. 2012
    ..The mould Alternaria alternata is a major elicitor of allergic asthma. Diagnosis and specific immunotherapy (SIT) of Alternaria allergy are often limited by the insufficient quality of natural mould extracts...
  14. doi request reprint Molecular characterization of wheat allergens specifically recognized by patients suffering from wheat-induced respiratory allergy
    S Pahr
    Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna General Hospital, Waehringer Guertel 18 20, Vienna, Austria
    Clin Exp Allergy 42:597-609. 2012
    ..Wheat (Triticum aestivum) is an important allergen source responsible for various clinical manifestations of allergy (i.e. food allergy, pollen allergy, respiratory allergy to flour-Baker's asthma)...
  15. doi request reprint Variability of IgE reactivity profiles among European mite allergic patients
    M Weghofer
    Division of Immunopathology, Department of Pathophysiology, Centre for Physiology and Pathophysiology, Medical University of Vienna, Austria
    Eur J Clin Invest 38:959-65. 2008
    ..Our aim was to determine the frequencies of IgE reactivity to purified HDM allergen molecules in mite allergic patients from different parts of Europe in order to establish an allergen panel for diagnosis of HDM allergy...
  16. doi request reprint Patients suffering from non-IgE-mediated cow's milk protein intolerance cannot be diagnosed based on IgG subclass or IgA responses to milk allergens
    H Hochwallner
    Department of Medical and Chemical Laboratory Diagnostics, Medical University of Vienna, Vienna, Austria
    Allergy 66:1201-7. 2011
    ....
  17. ncbi request reprint Comparison of purified Dermatophagoides pteronyssinus allergens and extract by two-dimensional immunoblotting and quantitative immunoglobulin E inhibitions
    M Weghofer
    Division of Immunopathology, Department of Pathophysiology, Center for Physiology and Pathophysiology, Medical University of Vienna, Vienna, Austria
    Clin Exp Allergy 35:1384-91. 2005
    ..These variations may influence allergenic activity and thus may have impact on diagnostic tests and specific immunotherapy...
  18. ncbi request reprint Characterization of recombinant cat albumin
    R Reininger
    Institute of Medical and Chemical Laboratory Diagnostics, University of Vienna, Vienna, Austria
    Clin Exp Allergy 33:1695-702. 2003
    ..Indoor allergens derived from animals and mites often contribute to exacerbation of skin manifestations in atopic dermatitis (AD) patients...
  19. ncbi request reprint Detection of an allergen in dog dander that cross-reacts with the major cat allergen, Fel d 1
    R Reininger
    Clinical Institute for Medical and Chemical Laboratory Diagnostics, Medical University of Vienna, Vienna, Austria
    Clin Exp Allergy 37:116-24. 2007
    ..A considerable proportion of animal-allergic patients are sensitized to both cat and dog allergens but knowledge about cross-reactive allergens in cat and dog dander is limited...
  20. doi request reprint Characterization of Der p 21, a new important allergen derived from the gut of house dust mites
    M Weghofer
    Division of Immunopathology, Department of Pathophysiology, Center for Physiology and Pathophysiology, Medical University of Vienna, Vienna, Austria
    Allergy 63:758-67. 2008
    ..The house dust mite (HDM) Dermatophagoides pteronyssinus is a major allergen source eliciting allergic asthma. The aim of the study was to identify new important HDM allergens associated with allergic asthma...
  21. ncbi request reprint IgA cross-reactivity between a nuclear autoantigen and wheat proteins suggests molecular mimicry as a possible pathomechanism in celiac disease
    S Natter
    Department of Pathophysiology, AKH, University of Vienna, Vienna, Austria
    Eur J Immunol 31:918-28. 2001
    ....
  22. doi request reprint The majority of allergen-specific IgE in the blood of allergic patients does not originate from blood-derived B cells or plasma cells
    J Eckl-Dorna
    Department of Otorhinolaryngology, Medical University of Vienna, Vienna, Austria
    Clin Exp Allergy 42:1347-55. 2012
    ..The production of allergen-specific IgE antibodies is a hallmark of IgE-mediated allergy but the contribution of blood cells to allergen-specific IgE production in allergic patients has not been studied in detail...
  23. doi request reprint Molecular characterization of Der p 10: a diagnostic marker for broad sensitization in house dust mite allergy
    Y Resch
    Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, AustriaAllergy Centre Vienna West, Vienna, Austria
    Clin Exp Allergy 41:1468-77. 2011
    ..Tropomyosins represent clinically relevant seafood allergens but the role of mite tropomyosin, Der p 10, in house dust mite (HDM) allergy has not been studied in detail...
  24. doi request reprint Association of allergic patients' phenotypes with IgE reactivity to recombinant pollen marker allergens
    A Twardosz-Kropfmüller
    Division of Immunopathology, Department of Pathophysiology, Center for Physiology and Pathophysiology, Medical University of Vienna, Vienna, Austria
    Allergy 65:296-303. 2010
    ..The aim of this study was to investigate whether IgE reactivity to recombinant pollen allergens with broad and narrow cross-reactivity is associated with clinical phenotypes of allergic sensitization...
  25. doi request reprint Analysis of the antibody responses induced by subcutaneous injection immunotherapy with birch and Fagales pollen extracts adsorbed onto aluminum hydroxide
    E Gadermaier
    Division of Immunopathology, Department of Pathophysiology, Center for Physiology and Pathophysiology, Medical University of Vienna, Vienna General Hospital, Vienna, Austria
    Int Arch Allergy Immunol 151:17-27. 2010
    ..In the present study, we analyze the epitope specificities of antibody responses induced by SCIT with allergen extracts from pollen of trees belonging to the order Fagales (birch, alder, hazel) adsorbed onto aluminum hydroxide...
  26. ncbi request reprint A molecular model of type I allergy: identification and characterization of a nonanaphylactic anti-human IgE antibody fragment that blocks the IgE-FcepsilonRI interaction and reacts with receptor-bound IgE
    S Laffer
    Department of Pathophysiology, AKH, University of Vienna, Vienna, Austria
    J Allergy Clin Immunol 108:409-16. 2001
    ..CONCLUSION: Fab12 represents a candidate molecule for therapy of atopy and asthma because it can be used for the depletion of circulating IgE antibodies, as well as for the depletion of IgE-bearing cells...
  27. ncbi request reprint Allergen-specific immunotherapy with a monophosphoryl lipid A-adjuvanted vaccine: reduced seasonally boosted immunoglobulin E production and inhibition of basophil histamine release by therapy-induced blocking antibodies
    N Mothes
    Department of Pathophysiology, Vienna General Hospital, University of Vienna, Vienna, Austria
    Clin Exp Allergy 33:1198-208. 2003
    ..The allergen extract-based analyses of immunotherapy-induced effects yielded highly controversial results regarding a beneficial role of therapy-induced IgG antibodies...
  28. ncbi request reprint The immunoglobulin E-allergen interaction: a target for therapy of type I allergic diseases
    R Valenta
    Department of General and Experimental Pathology, University of Vienna, Austria
    Int Arch Allergy Immunol 116:167-76. 1998
    ..Here we discuss active and passive strategies for therapy of type I allergy, which are based on interfering with the IgE-allergen interaction...
  29. doi request reprint A high-affinity monoclonal anti-IgE antibody for depletion of IgE and IgE-bearing cells
    S Laffer
    Division of Immunopathology, Department of Pathophysiology, Center for Physiology and Pathophysiology, Medical University of Vienna, Vienna, Austria
    Allergy 63:695-702. 2008
    ..In this study, we assessed the binding kinetics and affinity of monoclonal antibody 12 (mAb12) for IgE and investigated whether mAb12 can be used for depletion of IgE and isolation of IgE-bearing cells from peripheral blood...
  30. ncbi request reprint Skin test results but not serology reflect immediate type respiratory sensitivity: a study performed with recombinant allergen molecules
    V Niederberger
    Department of Otorhinolaryngology, University of Vienna, Austria
    J Invest Dermatol 117:848-51. 2001
    ..These results have implications for allergy diagnosis and, in particular, for the selection of relevant allergen components for specific immunotherapy...
  31. doi request reprint Developments in allergen-specific immunotherapy: from allergen extracts to allergy vaccines bypassing allergen-specific immunoglobulin E and T cell reactivity
    M Focke
    Christian Doppler Laboratory for Allergy Research, Division of Immunopathology, Department of Pathophysiology, Center for Pathophysiology, Immunology and Infectiology, Immunology and Infectiology, Medical University of Vienna, Vienna, Austria
    Clin Exp Allergy 40:385-97. 2010
    ..Should these peptide vaccines prove efficacious and safe in clinical trials, it may become possible to develop convenient, safe and broadly applicable forms of SIT as true alternatives to symptomatic, drug-based allergy treatment...
  32. ncbi request reprint Autoantibody reactivity in a case of Schnitzler's syndrome: evidence for a Th1-like response and detection of IgG2 anti-FcepsilonRIalpha antibodies
    W R Sperr
    Department of Internal Medicine I, Division of Hematology and Hemostaseology, University of Vienna, Austria
    Int Arch Allergy Immunol 122:279-86. 2000
    ..Whether such autoantibodies are recurrently produced in patients with Schnitzler's syndrome and play a role in the pathophysiology of the disease remains to be determined...
  33. ncbi request reprint Immunotherapy of allergic disease
    R Valenta
    Division of Immunopathology, Department of Pathophysiology, University of Vienna, Medical School, Austria
    Adv Immunol 82:105-53. 2004
  34. ncbi request reprint Molecular and immunological characterization of arginine kinase from the Indianmeal moth, Plodia interpunctella, a novel cross-reactive invertebrate pan-allergen
    M Binder
    Division of Specific Prophylaxis and Tropical Medicine and Immunopathology, Department of Pathophysiology, University of Vienna, Vienna, Austria
    J Immunol 167:5470-7. 2001
    ..Recombinant arginine kinase may be used to identify a group of polysensitized indoor allergic patients and for immunotherapy of these individuals...
  35. doi request reprint Basophils are not the key antigen-presenting cells in allergic patients
    J Eckl-Dorna
    Department of Otorhinolaryngology, Medical University of Vienna, AKH, Vienna, Austria
    Allergy 67:601-8. 2012
    ..Here, we investigate whether basophils are of importance for the presentation of allergen and the induction of T cell proliferation in allergic patients...
  36. doi request reprint The allergen profile of beech and oak pollen
    C Egger
    Christian Doppler Laboratory for Allergy Research, Division of Immunopathology, Department of Pathophysiology, Center for Physiology and Pathophysiology, Medical University of Vienna, Vienna, Austria
    Clin Exp Allergy 38:1688-96. 2008
    ..Beech and oak pollen are potential allergen sources with a world-wide distribution...
  37. doi request reprint Microarray and allergenic activity assessment of milk allergens
    H Hochwallner
    Department of Medical and Chemical Laboratory Diagnostics, Medical University of Vienna, Vienna, Austria
    Clin Exp Allergy 40:1809-18. 2010
    ..5% of infants in the first years of their life. However, only limited information regarding the allergenic activity of individual cow's milk allergens is available...
  38. doi request reprint Molecular composition and biological activity of commercial birch pollen allergen extracts
    M Focke
    Medical University of Vienna, Vienna, Austria
    Eur J Clin Invest 39:429-36. 2009
    ..The aim of this study was to analyse birch pollen allergen extracts produced for in vivo diagnosis of birch pollen allergy regarding their contents of individual birch pollen allergens (Bet v 1, Bet v 2 and Bet v 4)...
  39. ncbi request reprint Component-resolved diagnosis of house-dust mite allergy with purified natural and recombinant mite allergens
    G Pittner
    Division of Immunopathology, Department of Pathophysiology, Vienna General Hospital, Medical University of Vienna, Austria
    Clin Exp Allergy 34:597-603. 2004
    ..Immunotherapy with mite allergen extracts is frequently performed if allergen avoidance is not possible or successful. However, highly controversial results have been reported for mite-specific immunotherapy...
  40. ncbi request reprint The role of Foxp3+ T cells in long-term efficacy of prophylactic and therapeutic mucosal tolerance induction in mice
    B Winkler
    Department of Specific Prophylaxis and Tropical Medicine, Center for Physiology and Pathophysiology, Medical University of Vienna, Vienna, Austria
    Allergy 61:173-80. 2006
    ....
  41. ncbi request reprint Recombinant allergens promote expression of aminopeptidase-n (CD13) on basophils in allergic patients
    K Sonneck
    Department of Internal Medicine I, Division of Hematology and Hemostaseology, Medical University of Vienna, Vienna, Austria
    Int J Immunopathol Pharmacol 21:11-21. 2008
    ..In aggregate, our data show that recombinant allergens promote expression of CD13 on basophils in sensitized individuals. The functional significance and diagnostic implications of this observation remain to be determined...
  42. ncbi request reprint Identification of oilseed rape (Brassica napus) pollen profilin as a cross-reactive allergen
    Margarete Focke
    FAZ Floridsdorf Allergy Centre, University of Vienna, Vienna, Austria
    Int Arch Allergy Immunol 132:116-23. 2003
    ..Major allergens of oilseed rape (OSR) pollen with molecular weights of 6/8, 14 and between 27 and 69 kD have been described. The aim of the present study was to further characterize the 14-kD allergen...
  43. doi request reprint Heterogeneity of commercial timothy grass pollen extracts
    M Focke
    Christian Doppler Laboratory for Allergy Research, Department of Pathophysiology, Center for Physiology and Pathophysiology, Vienna General Hospital, Medical University of Vienna, Austria
    Clin Exp Allergy 38:1400-8. 2008
    ..The diagnosis and specific immunotherapy of allergy is currently performed with allergen extracts prepared from natural allergen sources...
  44. ncbi request reprint Analysis of the sensitization profile towards allergens in central Africa
    K Westritschnig
    Department of Pathophysiology, University of Vienna, Vienna, Austria
    Clin Exp Allergy 33:22-7. 2003
    ..Almost no information is available regarding the prevalence of IgE-mediated allergies and the disease-eliciting allergens in tropical Africa...
  45. ncbi request reprint The allergen profile of ash (Fraxinus excelsior) pollen: cross-reactivity with allergens from various plant species
    V Niederberger
    Department of Otorhinolaryngology, Vienna General Hospital, University of Vienna, Vienna, Austria
    Clin Exp Allergy 32:933-41. 2002
    ..Ash, a wind-pollinated tree belonging to the family Oleaceae, is distributed world-wide and has been suggested as a potent allergen source in spring time...
  46. doi request reprint Recombinant monoclonal human immunoglobulin E to investigate the allergenic activity of major grass pollen allergen Phl p 5
    C Madritsch
    Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria
    Clin Exp Allergy 41:270-80. 2011
    ..Allergen recognition by IgE antibodies is a key event in allergic inflammation...
  47. doi request reprint Micro-arrayed wheat seed and grass pollen allergens for component-resolved diagnosis
    C Constantin
    Division of Immunopathology, Department of Pathophysiology, Center of Physiology and Pathophysiology, Medical University of Vienna, Waehringer Guertel 18 20, Vienna, Austria
    Allergy 64:1030-7. 2009
    ..The aim of the study was to develop an allergen micro-array for differential diagnosis of baker's asthma, wheat-induced food allergy and grass pollen allergy...
  48. ncbi request reprint Genetic engineering of a hypoallergenic trimer of the major birch pollen allergen Bet v 1
    S Vrtala
    Department of Pathophysiology, Vienna General Hospital, University of Vienna Medical School, Waeringer Guertel 18-20, A-1090 Vienna, Austria
    FASEB J 15:2045-7. 2001
    ..Thus, rBet v 1 trimer represents a novel hypoallergenic vaccine prototype for treatment of one of the most frequent allergy forms...
  49. doi request reprint Inhibition of CD23-dependent facilitated allergen binding to B cells following vaccination with genetically modified hypoallergenic Bet v 1 molecules
    I Pree
    Department of Otorhinolaryngology, Medical University of Vienna, Wahringer Gurtel 18 20, Vienna, Austria
    Clin Exp Allergy 40:1346-52. 2010
    ..Vaccination with hypoallergenic recombinant Bet v 1 derivatives (Bet v 1 fragments and Bet v 1 trimer) is associated with the induction of IgG antibodies specific to natural Bet v 1...
  50. doi request reprint Antibodies induced with recombinant VP1 from human rhinovirus exhibit cross-neutralisation
    J Edlmayr
    Division of Immunopathology, Dept of Pathophysiology, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria
    Eur Respir J 37:44-52. 2011
    ..They exhibited strong cross-neutralising activity for different HRV strains. Therefore, recombinant VP1 may be considered a candidate HRV vaccine to prevent HRV-induced asthma exacerbations...
  51. ncbi request reprint Sensitization to human milk
    U Schulmeister
    Institute of Clinical and Medical Laboratory Diagnostics, Medical University of Vienna, Vienna, Austria
    Clin Exp Allergy 38:60-8. 2008
    ..Allergy to milk is one of the earliest manifestations of IgE-mediated allergies and affects about 2.5% of newborn children. Several reports indicate that milk-allergic patients may be sensitized also to human milk proteins...
  52. ncbi request reprint Molecular characterization of a human immunoglobulin G4 antibody specific for the major birch pollen allergen, Bet v 1
    S Flicker
    Division of Immunopathology, Department of Pathophysiology, Center for Physiology and Pathophysiology, Medical University of Vienna, Waehringer Guertel, Vienna, Austria
    Clin Exp Allergy 38:365-73. 2008
    ..Objective Our aim was the molecular characterization of a human IgG4 antibody (BAB5) specific for the major birch pollen allergen Bet v 1 that was derived from an immunotherapy-treated patient...
  53. ncbi request reprint Microarrayed recombinant allergens for diagnosis of allergy
    C Harwanegg
    VBC Genomics Bioscience Research GmbH, Vienna, Austria
    Clin Exp Allergy 33:7-13. 2003
    ..The wealth of diagnostic information gained through microarray-based allergy testing will likely improve diagnosis, prevention and treatment of allergy...
  54. doi request reprint Cigarette smoke facilitates allergen penetration across respiratory epithelium
    K Gangl
    Department of Otorhinolaryngology, Vienna General Hospital, Medical University of Vienna, Vienna, Austria
    Allergy 64:398-405. 2009
    ..We sought to investigate in an in vitro system whether active smoking reduces the integrity and barrier function of the respiratory epithelium and thus facilitates allergen penetration...
  55. pmc IgG subclass reactivity to human cardiac myosin in cardiomyopathy patients is indicative of a Th1-like autoimmune disease
    P Skyllouriotis
    General and Experimental Pathology, Vienna General Hospital, University of Vienna, Medical School, Vienna, Austria
    Clin Exp Immunol 115:236-47. 1999
    ....
  56. pmc Persistent molecular microchimerism induces long-term tolerance towards a clinically relevant respiratory allergen
    U Baranyi
    Division of Transplantation, Department of Surgery, Medical University of Vienna, Vienna, Austria
    Clin Exp Allergy 42:1282-92. 2012
    ..e. macrochimerism) and completely abrogated Phl p 5-specific immunity despite repeated immunizations with Phl p 5...
  57. pmc Immunoglobulin E antibody reactivity to bacterial antigens in atopic dermatitis patients
    K Reginald
    Department of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria
    Clin Exp Allergy 41:357-69. 2011
    ..AD patients are often sensitized against a broad variety of allergens and more than 90% of them suffer from skin superinfections with Staphylococcus aureus...
  58. ncbi request reprint Evaluation of the allergenic potential of Ginkgo biloba extracts
    R Mossabeb
    Department of Pathophysiology, University of Vienna, Austria
    Wien Klin Wochenschr 113:580-7. 2001
    ..In conclusion, no evidence for the presence of type I allergens in Ginkgo extracts was found. We recommend serological and/or skin testing to exclude sensitisation to components of Ginkgo biloba extracts...
  59. ncbi request reprint Component-resolved diagnosis to optimize allergen-specific immunotherapy in the Mediterranean area
    R Valenta
    Christian Doppler Laboratory for Allergy Research, Division of Immunopathology, Department of Pathophysiology, Center for Physiology and Pathophysiology, Medical University of Vienna, Austria
    J Investig Allergol Clin Immunol 17:36-40. 2007
    ..Here, we describe allergen molecules from these sources that can be used for component-resolved diagnosis of allergy to facilitate the selection of patients for SIT and monitor the immunological effects of treatment...
  60. pmc IgA autoreactivity: a feature common to inflammatory bowel and connective tissue diseases
    L Kazemi-Shirazi
    Department of Internal Medicine IV, Division of Gastroenterology and Hepatology, University of Vienna, Austria
    Clin Exp Immunol 128:102-9. 2002
    ..Our data indicate immunopathogenic similarities between IBD and CTD...
  61. ncbi request reprint Recombinant allergen-based concepts for diagnosis and therapy of type I allergy
    R Valenta
    Department of Pathophysiology, Vienna General Hospital AKH, University of Vienna Medical School, Waehringer Guertel 18 20, A 1090 Vienna, Austria
    Allergy 57:66-7. 2002
  62. ncbi request reprint Possible modes of allergen-specific sensitization and boosting in an atopic child
    R Reininger
    Clinical Institute for Medical and Chemical Laboratory Diagnostics, University of Vienna, Austria
    Int Arch Allergy Immunol 130:275-9. 2003
    ....
  63. ncbi request reprint Escherichia coli expression and purification of recombinant dog albumin, a cross-reactive animal allergen
    B Pandjaitan
    Institute of Medical and Chemical Laboratory Diagnostics, AKH, University of Vienna, Vienna, Austria
    J Allergy Clin Immunol 105:279-85. 2000
    ..Diagnosis and therapy of animal allergy would benefit from the availability of defined recombinant allergens. They may be preferred to animal-derived proteins, particularly for in vivo application in patients...
  64. ncbi request reprint Recombinant fish parvalbumins: Candidates for diagnosis and treatment of fish allergy
    Ines Swoboda
    Institute of Medical and Chemical Laboratory Diagnostics, AKH, University of Vienna, Wahringer Gurtel 18 20, A 1090 Vienna, Austria
    Allergy 57:94-6. 2002
    ..These clones will be the basis for the production of recombinant carp parvalbumin, a useful tool for in vitro and in vivo diagnosis of fish allergy...
  65. ncbi request reprint Molecular characterization of an autoallergen, Hom s 1, identified by serum IgE from atopic dermatitis patients
    R Valenta
    Institute of General and Experimental Pathology, Vienna General Hospital, University of Vienna Medical School, Austria
    J Invest Dermatol 111:1178-83. 1998
    ..If so, the respective recombinant molecule may serve as a paradigmatic tool for the diagnosis and treatment of patients with "intrinsic" atopy...
  66. ncbi request reprint Expression of mast cell tryptase by myeloblasts in a group of patients with acute myeloid leukemia
    W R Sperr
    Department of Internal Medicine I, Division of Hematology and Hemostaseology, University of Vienna, Austria
    Blood 98:2200-9. 2001
    ..Blast cell persistence or regrowth was associated with a persistently elevated level or recurrent increase of tryptase. Together, tryptase is expressed in myeloblasts in a group of AML and may serve as a useful disease-related marker...
  67. ncbi request reprint Isolation of cDNA clones coding for IgE autoantigens with serum IgE from atopic dermatitis patients
    S Natter
    Institute of General and Experimental Pathology, AKH, University of Vienna, Austria
    FASEB J 12:1559-69. 1998
    ..Via binding to antigen presenting cells as well as to effector cells, IgE autoantigen immune complexes may contribute to exacerbation and/or perpetuation of severe atopic diseases even in the absence of exogenous allergens...
  68. ncbi request reprint Cerivastatin and atorvastatin inhibit IL-3-dependent differentiation and IgE-mediated histamine release in human basophils and downmodulate expression of the basophil-activation antigen CD203c/E-NPP3
    Yasamin Majlesi
    Department of Internal Medicine I, Division of Hematology and Hemostaseology, University of Vienna, Wahringer Gurtel 18 20, A 1090 Vienna, Austria
    J Leukoc Biol 73:107-17. 2003
    ..Together, these data identify cerivastatin and atorvastatin as novel inhibitors of growth and activation of human basophils...
  69. doi request reprint Characterization of folded recombinant Der p 5, a potential diagnostic marker allergen for house dust mite allergy
    Margit Weghofer
    Division of Immunopathology, Department of Pathophysiology, Center for Physiology and Pathophysiology, Medical University of Vienna, Vienna, Austria
    Int Arch Allergy Immunol 147:101-9. 2008
    ..The aim of this study was to produce, by recombinant DNA technology, a folded Der p 5 allergen for diagnostic, therapeutic and preventive purposes...
  70. ncbi request reprint Formation of disulfide bonds and homodimers of the major cat allergen Fel d 1 equivalent to the natural allergen by expression in Escherichia coli
    Hans Grönlund
    Unit of Clinical Immunology and Allergy, Karolinska Institutet, Stockholm, Sweden
    J Biol Chem 278:40144-51. 2003
    ..Moreover, the recombinant Fel d 1 construct provides a tool for defining the three-dimensional structure of Fel d 1 and represents a reagent for diagnosis and allergen-specific immunotherapy of cat allergy...
  71. doi request reprint A hypoallergenic hybrid molecule with increased immunogenicity consisting of derivatives of the major grass pollen allergens, Phl p 2 and Phl p 6
    Birgit Linhart
    Division of Immunopathology, Department of Pathophysiology, Center of Physiology, Pathophysiology and Immunology, Medical University of Vienna, Vienna, Austria
    Biol Chem 389:925-33. 2008
    ..These antibodies could inhibit patients' IgE binding to the wild-type allergens. Thus, the described strategy allows the development of safer and more efficacious vaccines for the treatment of grass pollen allergy...
  72. ncbi request reprint Identification of cross-reactive and genuine Parietaria judaica pollen allergens
    Sabine Stumvoll
    Department of Pathophysiology, AKH, University of Vienna, Vienna, Austria
    J Allergy Clin Immunol 111:974-9. 2003
    ..The weed Parietaria judaica is one of the most important pollen allergen sources in the Mediterranean area...
  73. doi request reprint Reduction of the in vivo allergenicity of Der p 2, the major house-dust mite allergen, by genetic engineering
    Kuan Wei Chen
    Department of Pathophysiology, Center for Physiology and Pathophysiology, Medical University of Vienna, Waehringer Guertel 18 20, 1090 Vienna, Austria
    Mol Immunol 45:2486-98. 2008
    ..Thus the rDer p 2 derivatives exhibited less in vivo allergenic activity and allergenicity than the Der p 2 allergen but preserved immunogenicity and may hence represent candidates for specific immunotherapy of house-dust mite allergy...
  74. ncbi request reprint Prevention of allergen-specific IgE production and suppression of an established Th2-type response by immunization with DNA encoding hypoallergenic allergen derivatives of Bet v 1, the major birch-pollen allergen
    Romana Hochreiter
    Institute of Chemistry and Biochemistry, University of Salzburg, Austria
    Eur J Immunol 33:1667-76. 2003
    ..This concept may be applied for the development of safe and specific DNA vaccines for the prophylaxis and therapy of allergic diseases...
  75. ncbi request reprint Three-dimensional structure of the cross-reactive pollen allergen Che a 3: visualizing cross-reactivity on the molecular surfaces of weed, grass, and tree pollen allergens
    Petra Verdino
    Institute of Chemistry, Structural Biology, University of Graz, Heinrichstrasse 28, Graz, Austria
    J Immunol 180:2313-21. 2008
    ....
  76. ncbi request reprint Renaissance of the blocking antibody concept in type I allergy
    Sabine Flicker
    Division of Immunopathology, Department of Pathophysiology, University of Vienna, Vienna General Hospital, Vienna, Austria
    Int Arch Allergy Immunol 132:13-24. 2003
    ..In addition, passive administration of blocking antibodies may be considered as a potential therapeutic strategy for allergic diseases...
  77. pmc Carbohydrate-based particles: a new adjuvant for allergen-specific immunotherapy
    Hans Grönlund
    Unit of Clinical Immunology and Allergy, Karolinska Institutet, Stockholm, Sweden
    Immunology 107:523-9. 2002
    ..Based on these results, CBP is suggested as a potentially useful adjuvant for specific immunotherapy of IgE-mediated allergies...
  78. pmc Studies on the association between immunoglobulin E autoreactivity and immunoglobulin E-dependent histamine-releasing factors
    Ilona Kleine Budde
    Department of Immunopathology, Sanquin Research at CLB, Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Plesmanlaan 125, Amsterdam, The Netherlands
    Immunology 107:243-51. 2002
    ..A 20 000 MW IgE-defined autoantigen cross-reacted with a shrimp allergen. Our results indicate that IgE-reactivity to immunoblotted human protein and IgE-dependent HRF activity are distinct entities that may co-occur in atopic patients...
  79. ncbi request reprint Different IgE reactivity profiles in birch pollen-sensitive patients from six European populations revealed by recombinant allergens: an imprint of local sensitization
    Robert Movérare
    Department of Medical Sciences, Respiratory Medicine and Allergology, Uppsala University, Uppsala, Sweden
    Int Arch Allergy Immunol 128:325-35. 2002
    ..In this in vitro study, we examined the specific serum IgE binding profiles to individual birch pollen allergens in birch-sensitive patients from six European populations...
  80. ncbi request reprint Mutants of the major ryegrass pollen allergen, Lol p 5, with reduced IgE-binding capacity: candidates for grass pollen-specific immunotherapy
    Ines Swoboda
    Plant Molecular Biology and Biotechnology Laboratory, Institute of Land and Food Resources, The University of Melbourne, Parkville, Australia
    Eur J Immunol 32:270-80. 2002
    ..Our results demonstrate that a few point mutations in the Lol p 5 sequence yield mutants with reduced allergenic activity that represent potential vaccine candidates for immunotherapy of grass pollen allergy...
  81. ncbi request reprint Microarrayed allergen molecules: diagnostic gatekeepers for allergy treatment
    Reinhard Hiller
    VBC GENOMICS, A 1030 Vienna, Austria
    FASEB J 16:414-6. 2002
    ..This method may change established practice in allergy diagnosis, prevention, and therapy. In addition, microarrayed antigens may be applied to the diagnosis of autoimmune and infectious diseases...
  82. ncbi request reprint Evolution of IgM, IgE and IgG(1-4 )antibody responses in early childhood monitored with recombinant allergen components: implications for class switch mechanisms
    Verena Niederberger
    Department of Otorhinolaryngology, Vienna General Hospital, University of Vienna, Waeringer Guertel 18 20, A 1090 Vienna, Austria
    Eur J Immunol 32:576-84. 2002
    ....
  83. ncbi request reprint Molecular, structural, and immunologic relationships between different families of recombinant calcium-binding pollen allergens
    Raffaella Tinghino
    Department of Immunology, Istituto Superiore di Sanita, Rome
    J Allergy Clin Immunol 109:314-20. 2002
    ..Calcium-binding plant allergens can be grouped in different families according to the number of calcium-binding domains (EF hands)...
  84. ncbi request reprint Recombinant carp parvalbumin, the major cross-reactive fish allergen: a tool for diagnosis and therapy of fish allergy
    Ines Swoboda
    Institute of Medical and Chemical Laboratory Diagnostics, University of Vienna, Vienna, Austria
    J Immunol 168:4576-84. 2002
    ..01. The latter results suggest that it will be possible to develop strategies for immunotherapy for fish allergy that are based on calcium-free hypoallergenic rCyp c 1.01 derivatives...
  85. ncbi request reprint Recombinant marker allergens: diagnostic gatekeepers for the treatment of allergy
    Lili Kazemi-Shirazi
    Division of Gastroenterology and Hepatology, Department of Internal Medicine IV, University of Vienna Medical School, Wahringer Gurtel 18 20, A 1090 Vienna, Austria
    Int Arch Allergy Immunol 127:259-68. 2002
    ..Drawing from those examples, it is suggested how diagnostic tests based on such recombinant marker allergens may be used to improve the choice and monitoring of currently available forms of specific immunotherapy...
  86. ncbi request reprint The future of antigen-specific immunotherapy of allergy
    Rudolf Valenta
    Department of Pathophysiology, University of Vienna Medical School, Vienna General Hospital AKH, Australia
    Nat Rev Immunol 2:446-53. 2002
    ..It is proposed that these modified molecules will improve the current practice of specific immunotherapy and form a basis for prophylactic vaccination...
  87. ncbi request reprint Recombinant allergens promote expression of CD203c on basophils in sensitized individuals
    Alexander W Hauswirth
    Department of Internal Medicine I, Division of Hematology and Hemostaseology, University of Vienna, Vienna, Austria
    J Allergy Clin Immunol 110:102-9. 2002
    ..During the past few years, several attempts have been made to refine diagnostic assays in clinical allergy by introducing recombinant allergens and novel markers of IgE-dependent cell activation...
  88. ncbi request reprint Synthetic and genetically engineered allergen derivatives for specific immunotherapy of type I allergy
    Rudolf Valenta
    Vienna General Hospital AKH, University of Vienna Medical School, Vienna, Austria
    Clin Allergy Immunol 16:495-517. 2002
  89. ncbi request reprint Transition from a botanical to a molecular classification in tree pollen allergy: implications for diagnosis and therapy
    Nadine Mothes
    Center for Physiology and Pathophysiology, Department of Pathophysiology, Vienna General Hospital, Medical University of Vienna, Vienna, Austria
    Int Arch Allergy Immunol 135:357-73. 2004
    ..Information obtained by diagnostic testing with marker allergens will be important for the appropriate selection of patients for allergen-specific forms of therapy...
  90. ncbi request reprint Can we genetically engineer safer and more effective immunotherapy reagents?
    Kerstin Westritschnig
    Department of Pathophysiology, Vienna General Hospital, University of Vienna, Austria
    Curr Opin Allergy Clin Immunol 3:495-500. 2003
    ..Today the genetic informations for the most common allergens have been obtained. Here we review recombinant allergen-based technologies for the improvement of diagnosis and therapy of allergy...
  91. ncbi request reprint A hypoallergenic vaccine obtained by tail-to-head restructuring of timothy grass pollen profilin, Phl p 12, for the treatment of cross-sensitization to profilin
    Kerstin Westritschnig
    Christian Doppler Laboratory for Allergy Research, Division of Immunopathology, Department of Pathophysiology, Center for Physiology and Pathophysiology, Medical University of Vienna, Vienna, Austria
    J Immunol 179:7624-34. 2007
    ..The strategy of tail-to-head reassembly of hypoallergenic allergen fragments within one molecule represents a generally applicable strategy for the generation of allergy vaccines...
  92. ncbi request reprint Assays for measuring in vitro basophil activation induced by recombinant allergens
    Peter Valent
    Division of Hematology and Hemostaseology, Department of Internal Medicine I, The University of Vienna, Vienna, Austria
    Methods 32:265-70. 2004
    ..In the current article, the biochemical, functional, and technical background of these basophil tests is discussed...
  93. ncbi request reprint Characterization of wild-type recombinant Bet v 1a as a candidate vaccine against birch pollen allergy
    Thierry Batard
    Stallergènes, R and D, Antony, France
    Int Arch Allergy Immunol 136:239-49. 2005
    ..We describe the production in Escherichia coli as a recombinant protein of clinical grade wild-type Bet v 1a (rBet v 1a), to be used as a candidate vaccine against birch pollen allergy...
  94. ncbi request reprint IFN-gamma-enhanced allergen penetration across respiratory epithelium augments allergic inflammation
    Jürgen Reisinger
    Department of Otorhinolaryngology, Medical University of Vienna, Austria
    J Allergy Clin Immunol 115:973-81. 2005
    ..Respiratory allergen contact is the critical event in the elicitation and boosting of allergen-specific immune responses, as well as in the induction of immediate and late inflammatory reactions...
  95. ncbi request reprint A hybrid molecule resembling the epitope spectrum of grass pollen for allergy vaccination
    Birgit Linhart
    Cenetr of Physiology and Pathophysiology, Department of Pathophysiology, Medical University of Vienna, Austria
    J Allergy Clin Immunol 115:1010-6. 2005
    ..Allergy vaccines based on natural allergen extracts contain greatly varying amounts of individual allergens with different immunogenicity...
  96. ncbi request reprint Allergy testing: the role of recombinant allergens
    Nadine Mothes
    Zentrum für Physiologie und Pathophysiologie, Institut fur Pathophysiologie, Abteilung Immunpathologie, Medizinische Universität Wien, Vienna, Austria
    Clin Chem Lab Med 44:125-32. 2006
    ..Besides their diagnostic application, recombinant allergens and hypoallergenic derivatives thereof have also been used as vaccines in clinical trials, and recent results have shown their usefulness for the treatment of type I allergy...
  97. ncbi request reprint Generation of an allergy vaccine by disruption of the three-dimensional structure of the cross-reactive calcium-binding allergen, Phl p 7
    Kerstin Westritschnig
    Department of Pathophysiology, Vienna General Hospital, AKH, Medical University of Vienna, Vienna, Austria
    J Immunol 172:5684-92. 2004
    ....
  98. ncbi request reprint Identification of a villin-related tobacco protein as a novel cross-reactive plant allergen
    Irene Mittermann
    Division of Immunopathology, Department of Pathophysiology, Center for Physiology and Pathophysiology, Medical University of Vienna, Waehringer Guertel 18 20, A 1090 Vienna, Austria
    FEBS Lett 579:3807-13. 2005
    ..They may be used for the diagnosis and treatment of patients suffering from multivalent plant allergies...
  99. ncbi request reprint Hom s 4, an IgE-reactive autoantigen belonging to a new subfamily of calcium-binding proteins, can induce Th cell type 1-mediated autoreactivity
    Karl J Aichberger
    Division of Immunopathology, Department of Pathophysiology, Center for Physiology and Pathophysiology, Vienna General Hospital, Medical University of Vienna, Waehringer Guertel 18 20, A 1090 Vienna, Austria
    J Immunol 175:1286-94. 2005
    ..It may thus contribute to chronic skin inflammation in atopic as well as in nonatopic persons...
  100. ncbi request reprint Molecular design of allergy vaccines
    Birgit Linhart
    Division of Immunopathology, Department of Pathophysiology, Center for Physiology and Pathophysiology, Medical University of Vienna, Waehringer Guertel 18 20, A 1090 Vienna, Austria
    Curr Opin Immunol 17:646-55. 2005
    ....
  101. ncbi request reprint Gain of structure and IgE epitopes by eukaryotic expression of the major Timothy grass pollen allergen, Phl p 1
    Tanja Ball
    Department of Pathophysiology, Center for Physiology and Pathophysiology, Medical University of Vienna, Austria
    FEBS J 272:217-27. 2005
    ..This study emphasizes the important contribution of conformational epitopes for the IgE recognition of respiratory allergens and provides a paradigmatic tool for the structural analysis of the IgE allergen interaction...