Thu Ha Nguyen
Affiliation: University of Natural Resources and Applied Life Sciences
Kittibunchakul S, Pham M, Tran A, Nguyen T. β-Galactosidase from Lactobacillus helveticus DSM 20075: Biochemical Characterization and Recombinant Expression for Applications in Dairy Industry. Int J Mol Sci. 2019;20: pubmed publisher
..coli revealed high transgalactosylation activity of β-galactosidases from L. helveticus; hence, this enzyme is an interesting candidate for applications in lactose conversion and GOS formation processes. ..
Nguyen T, Nguyen H, Geiger B, Mathiesen G, Eijsink V, Peterbauer C, et al
. Heterologous expression of a recombinant lactobacillal Î²-galactosidase in Lactobacillus plantarum: effect of different parameters on the sakacin P-based expression system. Microb Cell Fact. 2015;14:30 pubmed publisher
..The results provide more detailed insight into these lactobacillal expression systems and confirm the potential of the pSIP system for efficient, tightly controlled expression of enzymes and proteins in lactobacilli. ..
Nguyen H, Mathiesen G, Stelzer E, Pham M, Kuczkowska K, Mackenzie A, et al
. Display of a Î²-mannanase and a chitosanase on the cell surface of Lactobacillus plantarum towards the development of whole-cell biocatalysts. Microb Cell Fact. 2016;15:169 pubmed
..This approach, with the enzyme of interest being displayed on the cell surface of a food-grade organism, may also be applied in production processes relevant for food industry. ..
Schumann C, Michlmayr H, Eder R, del Hierro A, Kulbe K, Mathiesen G, et al
. Heterologous expression of Oenococcus oeni malolactic enzyme in Lactobacillus plantarum for improved malolactic fermentation. AMB Express. 2012;2:19 pubmed publisher
..plantarum cells converted in a modified wine 15% (0.4 g/l) of initial L-malic acid concentration in 2 days. In conclusion, recombinant L. plantarum cells expressing MLE accelerate the malolactic fermentation. ..