Kim Nasmyth

Summary

Affiliation: University of Vienna
Country: Austria

Publications

  1. ncbi request reprint How do so few control so many?
    Kim Nasmyth
    Research Institute of Molecular Pathology, Vienna
    Cell 120:739-46. 2005
  2. ncbi request reprint A physical assay for sister chromatid cohesion in vitro
    Dmitri Ivanov
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, Vienna, Austria
    Mol Cell 27:300-10. 2007
  3. ncbi request reprint A topological interaction between cohesin rings and a circular minichromosome
    Dmitri Ivanov
    Research Institute of Molecular Pathology, Vienna, Austria
    Cell 122:849-60. 2005
  4. ncbi request reprint Kleisins: a superfamily of bacterial and eukaryotic SMC protein partners
    Alexander Schleiffer
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030, Vienna, Austria
    Mol Cell 11:571-5. 2003
  5. ncbi request reprint Division of the nucleolus and its release of CDC14 during anaphase of meiosis I depends on separase, SPO12, and SLK19
    Sara B C Buonomo
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030, Vienna, Austria
    Dev Cell 4:727-39. 2003
  6. pmc Shugoshin prevents dissociation of cohesin from centromeres during mitosis in vertebrate cells
    Barry E McGuinness
    Research Institute of Molecular Pathology, Vienna, Austria
    PLoS Biol 3:e86. 2005
  7. pmc Tandem affinity purification of functional TAP-tagged proteins from human cells
    Juraj Gregan
    Department of Chromosome Biology, Max F Perutz Laboratories, University of Vienna, Dr Bohr Gasse 1, 1030 Vienna, Austria
    Nat Protoc 2:1145-51. 2007
  8. ncbi request reprint The structure and function of SMC and kleisin complexes
    Kim Nasmyth
    Institute of Molecular Pathology, A 1030 Vienna, Austria
    Annu Rev Biochem 74:595-648. 2005
  9. pmc High-throughput knockout screen in fission yeast
    Juraj Gregan
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, 1030 Vienna, Austria
    Nat Protoc 1:2457-64. 2006
  10. pmc How might cohesin hold sister chromatids together?
    Kim Nasmyth
    IMP Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Philos Trans R Soc Lond B Biol Sci 360:483-96. 2005

Detail Information

Publications50

  1. ncbi request reprint How do so few control so many?
    Kim Nasmyth
    Research Institute of Molecular Pathology, Vienna
    Cell 120:739-46. 2005
    ..Recent experiments shed important insight into how Mad2 molecules bound to centromeres through their association with a protein called Mad1 might be transferred to Cdc20 and thereby inhibit securin's destruction...
  2. ncbi request reprint A physical assay for sister chromatid cohesion in vitro
    Dmitri Ivanov
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, Vienna, Austria
    Mol Cell 27:300-10. 2007
    ..These data represent the best evidence so far that the cohesin ring physically holds sister DNAs together and are consistent with the notion that it does so by using a topological principle involving the trapping DNAs inside its ring...
  3. ncbi request reprint A topological interaction between cohesin rings and a circular minichromosome
    Dmitri Ivanov
    Research Institute of Molecular Pathology, Vienna, Austria
    Cell 122:849-60. 2005
    ..This suggests that the stable association between cohesin and chromatin detected here is topological rather than physical, which is consistent with the notion that DNA is trapped inside cohesin rings...
  4. ncbi request reprint Kleisins: a superfamily of bacterial and eukaryotic SMC protein partners
    Alexander Schleiffer
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030, Vienna, Austria
    Mol Cell 11:571-5. 2003
    ..Scc1 interacts with SMC proteins through N- and C-terminal domains to form a ring-like structure. Since these are the only domains conserved among kleisins, we suggest that ring formation with SMC proteins may define this family...
  5. ncbi request reprint Division of the nucleolus and its release of CDC14 during anaphase of meiosis I depends on separase, SPO12, and SLK19
    Sara B C Buonomo
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030, Vienna, Austria
    Dev Cell 4:727-39. 2003
    ..It has two mechanistically different roles during meiosis I. Loss of the protease-independent function alone results in a second meiotic division occurring on anaphase I spindles in spo12delta and slk19delta mutants...
  6. pmc Shugoshin prevents dissociation of cohesin from centromeres during mitosis in vertebrate cells
    Barry E McGuinness
    Research Institute of Molecular Pathology, Vienna, Austria
    PLoS Biol 3:e86. 2005
    ..Centromeric cohesion is one of the hallmarks of mitotic chromosomes. Our results imply that it is not an intrinsically stable property, because it can easily be destroyed by mitotic kinases, which are kept in check by shugoshin...
  7. pmc Tandem affinity purification of functional TAP-tagged proteins from human cells
    Juraj Gregan
    Department of Chromosome Biology, Max F Perutz Laboratories, University of Vienna, Dr Bohr Gasse 1, 1030 Vienna, Austria
    Nat Protoc 2:1145-51. 2007
    ..The purification protocol can be completed in 1 day and it should be applicable to other proteins...
  8. ncbi request reprint The structure and function of SMC and kleisin complexes
    Kim Nasmyth
    Institute of Molecular Pathology, A 1030 Vienna, Austria
    Annu Rev Biochem 74:595-648. 2005
    ..Here we describe the discovery of SMC complexes and discuss recent advances in determining how members of this ancient protein family may function at a mechanistic level...
  9. pmc High-throughput knockout screen in fission yeast
    Juraj Gregan
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, 1030 Vienna, Austria
    Nat Protoc 1:2457-64. 2006
    ..Here, we present our protocol with detailed instructions. Using this protocol, one person can knock out 96 S. pombe genes in 8 days...
  10. pmc How might cohesin hold sister chromatids together?
    Kim Nasmyth
    IMP Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Philos Trans R Soc Lond B Biol Sci 360:483-96. 2005
    ..This paper reviews the evidence that cohesin is directly responsible for holding sister DNAs together and considers how it might perform this function in the light of recent data on its structure...
  11. pmc The kinetochore proteins Pcs1 and Mde4 and heterochromatin are required to prevent merotelic orientation
    Juraj Gregan
    Max F Perutz Laboratories, Department of Chromosome Biology, University of Vienna, Dr Bohr Gasse 1, 1030 Vienna, Austria
    Curr Biol 17:1190-200. 2007
    ..A protein called Pcs1 has been implicated in preventing merotelic attachment in mitosis and meiosis II in the fission yeast S. pombe...
  12. pmc Construction of conditional analog-sensitive kinase alleles in the fission yeast Schizosaccharomyces pombe
    Juraj Gregan
    Department of Chromosome Biology, Max F Perutz Laboratories, University of Vienna, Dr Bohr Gasse 1, 1030 Vienna, Austria
    Nat Protoc 2:2996-3000. 2007
    ..Here, we describe a protocol to construct conditional analog-sensitive kinase alleles in the fission yeast Schizosaccharomyces pombe. This protocol can be completed in about 3 weeks and should be applicable to other organisms as well...
  13. pmc From a single double helix to paired double helices and back
    Kim Nasmyth
    Research Institute for Molecular Biology IMP, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Philos Trans R Soc Lond B Biol Sci 359:99-108. 2004
    ..Cohesion between sister chromatids is destroyed at the metaphase to anaphase transition by proteolytic cleavage of cohesin's Scc1 subunit by a thiol protease called separase, which severs the ring and thereby releases sister DNAs...
  14. ncbi request reprint Eco1 is a novel acetyltransferase that can acetylate proteins involved in cohesion
    Dmitri Ivanov
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030, Vienna, Austria
    Curr Biol 12:323-8. 2002
    ..In vitro Eco1 acetylates itself and components of the cohesin complex but not histones. Thus, the establishment of cohesion between sister chromatids appears to be regulated, directly or indirectly, by a specific acetyltransferase...
  15. ncbi request reprint A REC8-dependent plant Shugoshin is required for maintenance of centromeric cohesion during meiosis and has no mitotic functions
    Olivier Hamant
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720, USA
    Curr Biol 15:948-54. 2005
    ..However, in contrast to other Shugoshins, we observed an early and REC8-dependent recruitment of ZmSGO1 in maize, suggesting that control of SGO1 recruitment to chromosomes is different in plants than in other model organisms...
  16. ncbi request reprint Novel genes required for meiotic chromosome segregation are identified by a high-throughput knockout screen in fission yeast
    Juraj Gregan
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Curr Biol 15:1663-9. 2005
    ..sgo1 is required for retention of centromeric sister-chromatid cohesion after anaphase I. We show here that mde2 is required for formation of the double-strand breaks necessary for meiotic recombination...
  17. ncbi request reprint Control of Shugoshin function during fission-yeast meiosis
    Sabine Vaur
    Centre National de la Recherche Scientifique, Institut de Biochimie et Génétique Cellulaires, Unité Mixte de Recherche 5095, Bordeaux, F 33077, France
    Curr Biol 15:2263-70. 2005
    ..Sgo1-mediated protection of centromere cohesion might therefore be regulated by the mode of kinetochore attachment...
  18. pmc Separase: a universal trigger for sister chromatid disjunction but not chromosome cycle progression
    Karin G Wirth
    Research Institute of Molecular Pathology, A 1030 Vienna, Austria
    J Cell Biol 172:847-60. 2006
    ..Separase depletion in bone marrow causes aplasia and the presumed death of hematopoietic cells other than erythrocytes. Destruction of sister chromatid cohesion by Separase may be a universal feature of mitosis in eukaryotic cells...
  19. ncbi request reprint Protein phosphatase 2A protects centromeric sister chromatid cohesion during meiosis I
    Christian G Riedel
    Research Institute of Molecular Pathology IMP, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Nature 441:53-61. 2006
    ..Our data are consistent with the notion that efficient cleavage of Rec8 requires phosphorylation of cohesin and that this is blocked by PP2A at meiosis I centromeres...
  20. ncbi request reprint Resolution of chiasmata in oocytes requires separase-mediated proteolysis
    Nobuaki R Kudo
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Cell 126:135-46. 2006
    ..Both types of mRNA restore PBE. Proteolytic activity of separase is therefore essential for Rec8's removal from chromosome arms and for chiasma resolution but not for PBE...
  21. ncbi request reprint Monopolar attachment of sister kinetochores at meiosis I requires casein kinase 1
    Mark Petronczki
    Research Institute of Molecular Pathology, Dr Bohrgasse 7, A 1030 Vienna, Austria
    Cell 126:1049-64. 2006
    ....
  22. ncbi request reprint Cohesin's ATPase activity is stimulated by the C-terminal Winged-Helix domain of its kleisin subunit
    Prakash Arumugam
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Curr Biol 16:1998-2008. 2006
    ..However, the precise role of the ATPase domains remains enigmatic...
  23. ncbi request reprint Evidence that loading of cohesin onto chromosomes involves opening of its SMC hinge
    Stephan Gruber
    Institute of Molecular Pathology, Dr Bohr Gasse 7, 1030 Vienna, Austria
    Cell 127:523-37. 2006
    ..Our results suggest that entry of DNA into cohesin's ring requires transient dissociation of Smc1 and Smc3 hinge domains...
  24. ncbi request reprint Human Scc4 is required for cohesin binding to chromatin, sister-chromatid cohesion, and mitotic progression
    Erwan Watrin
    Research Institute of Molecular Pathology I M P, Dr Bohr Gasse 7, 1030 Vienna, Austria
    Curr Biol 16:863-74. 2006
    ..Mutation of Scc2 orthologs causes defects in cohesion, transcription, and development, resulting in Cornelia de Lange syndrome in humans...
  25. pmc Cell-type-specific TEV protease cleavage reveals cohesin functions in Drosophila neurons
    Andrea Pauli
    Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK
    Dev Cell 14:239-51. 2008
    ..These data demonstrate essential roles for cohesin in nondividing cells and also introduce a powerful tool by which to investigate protein function in metazoa...
  26. doi request reprint The cohesin ring concatenates sister DNA molecules
    Christian H Haering
    University of Oxford, Department of Biochemistry, South Parks Road, Oxford OX1 3QU, UK
    Nature 454:297-301. 2008
    ..As predicted by the ring entrapment model, this procedure produced dimeric DNA-cohesin structures that are resistant to protein denaturation. We conclude that cohesin rings concatenate individual sister minichromosome DNA molecules...
  27. ncbi request reprint Spo13 facilitates monopolin recruitment to kinetochores and regulates maintenance of centromeric cohesion during yeast meiosis
    Vittorio L Katis
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Curr Biol 14:2183-96. 2004
    ..Crucially, cohesin at centromeres is retained to allow sister centromeres to biorient at the second division. Little is known about how these meiosis I-specific events are regulated...
  28. ncbi request reprint Building and breaking bridges between sister chromatids
    Christian H Haering
    Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Bioessays 25:1178-91. 2003
    ..New research suggests that these ancient regulators of chromosome structure might function as topological devices that trap chromosomal DNA between 50 nm long coiled coils...
  29. ncbi request reprint ATP hydrolysis is required for cohesin's association with chromosomes
    Prakash Arumugam
    Research Institute of Molecular Pathology IMP, Dr Bohr Gasse 7, 1030 Vienna, Austria
    Curr Biol 13:1941-53. 2003
    ..These Smc "heads" are connected by Scc1, creating a tripartite proteinaceous ring...
  30. ncbi request reprint Polo-like kinase Cdc5 promotes chiasmata formation and cosegregation of sister centromeres at meiosis I
    Rosemary K Clyne
    Research Institute of Molecular Pathology IMP, Vienna A 1030, Austria
    Nat Cell Biol 5:480-5. 2003
    ..3) and Mam1 (ref. 4) monopolin proteins, resulting in bipolar orientation of sister kinetochores. Cdc5 is thus required both for the formation of chiasmata and for cosegregation of sister centromeres at meiosis I...
  31. ncbi request reprint Kinetochore recruitment of two nucleolar proteins is required for homolog segregation in meiosis I
    Kirsten P Rabitsch
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Dev Cell 4:535-48. 2003
    ..Csm1 and Pcs1 might clamp together microtubule binding sites on the same (Pcs1) or sister (Csm1) kinetochores...
  32. ncbi request reprint Chromosomal cohesin forms a ring
    Stephan Gruber
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, 1030 Vienna, Austria
    Cell 112:765-77. 2003
    ..Cleavage of the Smc3 coiled coil is sufficient to trigger cohesin release from chromosomes and loss of sister cohesion, consistent with a topological association with chromatin...
  33. ncbi request reprint Un ménage à quatre: the molecular biology of chromosome segregation in meiosis
    Mark Petronczki
    Research Institute of Molecular Pathology, Dr Bohrgasse 7, 1030 Vienna, Austria
    Cell 112:423-40. 2003
    ....
  34. ncbi request reprint Evolution of eukaryotic cell cycle regulation: stepwise addition of regulatory kinases and late advent of the CDKs
    Dmitri M Krylov
    National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, 20894, Bethesda, MD, USA
    Curr Biol 13:173-7. 2003
    ..During subsequent evolution, a series of duplications of kinase genes occurred, gradually adding more kinases to the CCC system, the CDKs being among the last major additions...
  35. ncbi request reprint Segregating sister genomes: the molecular biology of chromosome separation
    Kim Nasmyth
    IMP, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Science 297:559-65. 2002
    ....
  36. ncbi request reprint Molecular architecture of SMC proteins and the yeast cohesin complex
    Christian H Haering
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Mol Cell 9:773-88. 2002
    ..The two heads of the V-shaped dimer are connected by different ends of the cleavable Scc1 subunit. Cohesin therefore forms a large proteinaceous loop within which sister chromatids might be entrapped after DNA replication...
  37. pmc Loss of the anaphase-promoting complex in quiescent cells causes unscheduled hepatocyte proliferation
    Karin G Wirth
    Research Institute of Molecular Pathology IMP, A 1030 Vienna, Austria
    Genes Dev 18:88-98. 2004
    ..Re-entry into the cell cycle either occurred without any proliferative stimulus or could be easily induced. We demonstrate that the APC has an additional function to prevent hepatocytes from unscheduled re-entry into the cell cycle...
  38. ncbi request reprint Tension between two kinetochores suffices for their bi-orientation on the mitotic spindle
    Hilary Dewar
    School of Life Sciences, University of Dundee, Wellcome Trust Biocentre, Dundee DD1 5EH, UK
    Nature 428:93-7. 2004
    ..We also show that Ipl1 facilitates bi-orientation by promoting the turnover of kinetochore-spindle pole connections in a tension-dependent manner...
  39. ncbi request reprint Two fission yeast homologs of Drosophila Mei-S332 are required for chromosome segregation during meiosis I and II
    Kirsten P Rabitsch
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Curr Biol 14:287-301. 2004
    ..Segregation during the second division depends on retention of sister chromatid cohesion between centromeres until the onset of anaphase II, which in Drosophila melanogaster depends on a protein called Mei-S332 that binds to centromeres...
  40. ncbi request reprint Structure and stability of cohesin's Smc1-kleisin interaction
    Christian H Haering
    Research Institute of Molecular Pathology IMP, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Mol Cell 15:951-64. 2004
    ..There is little or no turnover of Smc1-Scc1 interactions within cohesin complexes in vivo because expression of noncleavable Scc1 after DNA replication does not hinder anaphase...
  41. ncbi request reprint The Drosophila meiotic kleisin C(2)M functions before the meiotic divisions
    Doris Heidmann
    Department of Genetics, University of Bayreuth, 95440 Bayreuth, Germany
    Chromosoma 113:177-87. 2004
    ..Therefore, C(2)M appears to function within the synaptonemal complex during prophase I but possibly not thereafter. This suggests that C(2)M may not confer sister chromatid cohesion needed for meiosis I and II chromosome segregation...
  42. ncbi request reprint Evidence that the Ipl1-Sli15 (Aurora kinase-INCENP) complex promotes chromosome bi-orientation by altering kinetochore-spindle pole connections
    Tomoyuki U Tanaka
    School of Life Sciences, University of Dundee, MSI WTB complex, DD1 5EH, Dundee, United Kingdom
    Cell 108:317-29. 2002
    ..This raises the possibility that Ipl1-Sli15 facilitates bi-orientation by promoting turnover of kinetochore-SPB connections until traction of sister kinetochores toward opposite spindle poles creates tension in the surrounding chromatin...
  43. ncbi request reprint Phosphorylation by cyclin B-Cdk underlies release of mitotic exit activator Cdc14 from the nucleolus
    Ramzi Azzam
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Science 305:516-9. 2004
    ..Thus, a regulatory circuit exists to ensure that the arbiter of the mitotic state, Cdk, sets in motion events that culminate in exit from mitosis...
  44. ncbi request reprint Sister-chromatid cohesion mediated by the alternative RF-CCtf18/Dcc1/Ctf8, the helicase Chl1 and the polymerase-alpha-associated protein Ctf4 is essential for chromatid disjunction during meiosis II
    Mark Petronczki
    Research Institute of Molecular Pathology, Dr Bohrgasse 7, A 1030 Vienna, Austria
    J Cell Sci 117:3547-59. 2004
    ..Our finding that mutants in fission yeast ctf18 and dcc1 have similar defects suggests that the involvement of the alternative RF-C(Ctf18/Dcc1/Ctf8) complex in sister chromatid cohesion might be highly conserved...
  45. ncbi request reprint Maintenance of cohesin at centromeres after meiosis I in budding yeast requires a kinetochore-associated protein related to MEI-S332
    Vittorio L Katis
    Research Institute of Molecular Pathology, Dr Bohrgasse 7, A 1030 Vienna, Austria
    Curr Biol 14:560-72. 2004
    ..Third, cohesion between sister centromeres must persist after the first meiotic division to enable their biorientation at the second...
  46. ncbi request reprint Changing places. Interview by Graham Tebb
    Kim Nasmyth
    Curr Biol 14:R452-3. 2004
    b>Kim Nasmyth is set to move from the Institute of Molecular Pathology in Vienna to take up a new post in Oxford. He talks to Graham Tebb about his reasons for returning to the UK and plans for the future.
  47. ncbi request reprint A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II
    Henri Marc Bourbon
    Mol Cell 14:553-7. 2004
  48. pmc Introduction: dissection and simplification of chromosome segregation
    Mitsuhiro Yanagida
    Department of Gene Mechanism, Graduate School of Biostudies, Kyoto University, Yoshida Honmachi, Kyoto 606 8501, Japan
    Philos Trans R Soc Lond B Biol Sci 360:481. 2005
  49. pmc BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals
    Ina Poser
    Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D 01307 Dresden, Germany
    Nat Methods 5:409-15. 2008
    ..The same high-throughput approach will be generally applicable to other model systems...
  50. ncbi request reprint Is chromatin remodeling required to build sister-chromatid cohesion?
    Christian G Riedel
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Trends Biochem Sci 29:389-92. 2004
    ..It remains unclear whether they do so by facilitating the recruitment of cohesin to specific chromosomal sequences or by modifying an event at replication forks giving rise to cohesion between sister DNAs...