Diethard Mattanovich

..They are also popular in biotechnological processes, like heterologous protein production...

..Transcriptional analysis and data mining allowed for the selection of potential targets for strain improvement. A first selection of these candidates has been evaluated as recombinant protein secretion enhancers...

....

..g. recombinant protein production vs. non production conditions) are compared...

..Gene expression profiling was combined with proteomic analyses and the 13C isotope labelling based experimental determination of metabolic fluxes in the central carbon metabolism...

....

..abstract:..

....

..pastoris research, so that the Pichia community can benefit from exchange of knowledge, data and materials...

..abstract:..

..While our main intention was to analyze the effect of different osmolarities on P. pastoris in general, this was complemented by studying it in context with recombinant protein production...

..These data indicate that folding stress is generally decreased at lower cultivation temperatures, enabling more efficient heterologous protein secretion in P. pastoris host cells...

..5-fold in fed-batch fermentations of P. pastoris...

..pastoris and analyzed the unfolded protein response (UPR) in this yeast species, as compared to S. cerevisiae...

....

....

....

..pastoris directly without the hassle to modify standard protocols designed for the model organism S. cerevisiae first...

..These data clearly demonstrate that protein folding and ER stress have a severe impact on the cytosolic redox balance, which may be a major factor during development of folding-related diseases...

..3-fold, PDI enabled an increase of the Fab level by 1.9-fold. Hence, the formation of interchain disulfide bonds can be seen as a major rate limiting factor to Fab assembly and subsequent secretion...

..The microarray based monitoring of the enrichment of genes causing enhanced protein secretory capacity led to novel insights into the limitation of protein secretion...

..pastoris, human trypsinogen, human serum albumin and Rhizopus oryzae lipase, was evaluated. While the disruption had no effect on the secretion of trypsinogen and albumin, the amount of lipase released from the cells was doubled...

..This work will further facilitate our understanding how recombinant proteins behave in the secretory pathway...

..Biochemical analysis confirmed that the N-glycosylation does not contribute to the binding properties of 3H6...

....

....

..Now yeasts are very close to industrial exploitation - here we summarize the developments in this field...

..The best lactic-acid-producing strain was identified within the screening of CEN.PK m850 mutants...

..Another field of application for these truly prototrophic hosts is the production of biochemicals and small metabolites, where defined mineral media leads to easier purification procedures...

..The optimum case would be represented by metabolites, easy to extract from yeast with high and precise recovery. In other cases the total combined uncertainty was significantly higher...

..Overexpression of BIP, both alone and in combination with PDI, reduced the specific secretion rate, whereas PDI, when overexpressed alone, caused an increase of product secretion rate...

..The development of next generation sequencing technology enables an unexpected revival of genomic approaches, providing new potential for evolutionary engineering and inverse metabolic engineering...

..At the same time, it has become obvious that P. pastoris, as an evolutionarily more 'ancient' yeast, may in some cases be a better model for human cell biology and disease than Saccharomyces cerevisiae...

....

..The refolded trypsinogen was converted to trypsin by autocatalytic activation, and the utility for the detachment of mammalian cells in culture was proven...

..Similar questions arise for a variety of products if they are to be produced bio-based in large scale. However, special emphasis is given to 1,3-propanediol production...

..Notably, a different behavior was observed for exponentially grown and stationary cells in terms of the appearance and development of these subpopulations as a response to a changing external pH...

..Thus labeling procedures must be compatible with minimal production media and the usage of bioreactors. The inorganic and non-radioactive 34S labeled sodium sulfate meets both demands...

..Following aspects are addressed: folding, assembly and secretion of antibody related proteins, process optimization to improve productivity and quality, proteolysis, and, as a major point of interest, glycosylation...

..In this article, through analysis of the current advances in production of these acids, we present guidelines for future developments in this fast-moving field...

..Apart from discussing well-documented stress reactions of yeasts in the context of heterologous protein production, some more speculative topics like quorum sensing and apoptosis are addressed...

..We suggest to intensify the studies of the ruminal microbial ecosystem from an industrial microbiologists point of view in order to make use of this rich source of organisms and enzymes...

..g. glycerol, while heterologous gene expression should be active in the feed phase under carbon (e.g. glucose) limitation...

..Batch purification of a Fab fragment produced in the Δepx1 strain highlighted its superior purity due to the decreased host cell protein load...

..Additionally, the development of novel secretion systems was presented, giving one example of how to combine industry's needs with highly ambitious fundamental research...

..Contrary to previous results with roGFP, the optimized roGFP1_iE and roGFP1_iL constructs were not completely oxidized, and are therefore useful sensors for monitoring the ER under conditions when it is even more oxidized...

..Product titers from the detection limit to up to 570 mg/L proved that the set of constitutive promoters is a powerful tool for the fine-tuning of metabolic pathways for the improvement of industrial production processes...

..Sensitivity of LC-ICP-MS was 1-2 orders of magnitude lower than that of LC-MS-MS. Quantitative analysis of yeast products using complementary MS detection furnished values in good agreement...

..Furthermore, the addition of Vitamin B(12) to the culture medium increased production by 15% to a final titer of 84.5 g/l 1,3-propanediol...

....

..Furthermore, it is of biotechnological interest due to its capability of producing various volatile fatty acids. Here we report the complete genome sequence of M. elsdenii DSM 20460, the type strain for the species...

..pastoris...

..The clones obtained had a similar SOD production compared to the original strain, but were able to reach higher densities in a batch culture, which resulted in a higher total yield...