Diethard Mattanovich

Summary

Affiliation: University of Natural Resources and Applied Life Sciences
Country: Austria

Publications

  1. pmc Yeast biotechnology: teaching the old dog new tricks
    Diethard Mattanovich
    Department of Biotechnology, University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria
    Microb Cell Fact 13:34. 2014
  2. doi request reprint Editorial: metabolic modeling in biotechnology and medical research
    Diethard Mattanovich
    Department of Biotechnology, University of Natural Resources and Life Sciences Vienna, Austria Austrian Centre of Industrial Biotechnology, Vienna, Austria
    Biotechnol J 8:962-3. 2013
  3. pmc Repressible promoters - a novel tool to generate conditional mutants in Pichia pastoris
    Marizela Delic
    Department of Biotechnology, University of Natural Resources and Life Sciences Vienna BOKU, Muthgasse 18, 1190, Vienna, Austria
    Microb Cell Fact 12:6. 2013
  4. pmc Protein trafficking, ergosterol biosynthesis and membrane physics impact recombinant protein secretion in Pichia pastoris
    Kristin Baumann
    Department of Chemical Engineering, Universitat Autonoma de Barcelona, Bellaterra Cerdanyola del Valles, Spain
    Microb Cell Fact 10:93. 2011
  5. pmc Lactate production yield from engineered yeasts is dependent from the host background, the lactate dehydrogenase source and the lactate export
    Paola Branduardi
    Universita degli Studi di Milano Bicocca, Dipartimento di Biotecnologie e Bioscienze, P, zza della Scienza 2, 20126 Milano, Italy
    Microb Cell Fact 5:4. 2006
  6. pmc Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels
    Marc Carnicer
    Departament d Enginyeria Quimica, Escola d Enginyeria, Universitat Autonoma de Barcelona, Bellaterra, Spain
    Microb Cell Fact 8:65. 2009
  7. pmc A multi-level study of recombinant Pichia pastoris in different oxygen conditions
    Kristin Baumann
    Department of Chemical Engineering, Autonomous University of Barcelona, Spain
    BMC Syst Biol 4:141. 2010
  8. pmc The impact of oxygen on the transcriptome of recombinant S. cerevisiae and P. pastoris - a comparative analysis
    Kristin Baumann
    Department of Chemical Engineering, Autonomous University of Barcelona, Spain
    BMC Genomics 12:218. 2011
  9. pmc Overexpression of the riboflavin biosynthetic pathway in Pichia pastoris
    Hans Marx
    Institute of Applied Microbiology, Department of Biotechnology, BOKU University of Natural Resources and Applied Life Sciences, Muthgasse 18, 1190, Wien, Austria
    Microb Cell Fact 7:23. 2008
  10. pmc Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris
    Diethard Mattanovich
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria
    Microb Cell Fact 8:29. 2009

Collaborators

Detail Information

Publications68

  1. pmc Yeast biotechnology: teaching the old dog new tricks
    Diethard Mattanovich
    Department of Biotechnology, University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria
    Microb Cell Fact 13:34. 2014
    ..An overview of emerging and established substrates and products of yeast biotechnology is provided and discussed in the light of the recent literature. ..
  2. doi request reprint Editorial: metabolic modeling in biotechnology and medical research
    Diethard Mattanovich
    Department of Biotechnology, University of Natural Resources and Life Sciences Vienna, Austria Austrian Centre of Industrial Biotechnology, Vienna, Austria
    Biotechnol J 8:962-3. 2013
    Metabolic Modeling and Simulation: This special issue of Biotechnology Journal is edited by Diethard Mattanovich and Vassily Hatzimanikatis and covers the state-of-the-art in metabolic modeling, including the major themes of methods in ..
  3. pmc Repressible promoters - a novel tool to generate conditional mutants in Pichia pastoris
    Marizela Delic
    Department of Biotechnology, University of Natural Resources and Life Sciences Vienna BOKU, Muthgasse 18, 1190, Vienna, Austria
    Microb Cell Fact 12:6. 2013
    ..They are also popular in biotechnological processes, like heterologous protein production...
  4. pmc Protein trafficking, ergosterol biosynthesis and membrane physics impact recombinant protein secretion in Pichia pastoris
    Kristin Baumann
    Department of Chemical Engineering, Universitat Autonoma de Barcelona, Bellaterra Cerdanyola del Valles, Spain
    Microb Cell Fact 10:93. 2011
    ..Transcriptional analysis and data mining allowed for the selection of potential targets for strain improvement. A first selection of these candidates has been evaluated as recombinant protein secretion enhancers...
  5. pmc Lactate production yield from engineered yeasts is dependent from the host background, the lactate dehydrogenase source and the lactate export
    Paola Branduardi
    Universita degli Studi di Milano Bicocca, Dipartimento di Biotecnologie e Bioscienze, P, zza della Scienza 2, 20126 Milano, Italy
    Microb Cell Fact 5:4. 2006
    ....
  6. pmc Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels
    Marc Carnicer
    Departament d Enginyeria Quimica, Escola d Enginyeria, Universitat Autonoma de Barcelona, Bellaterra, Spain
    Microb Cell Fact 8:65. 2009
    ..g. recombinant protein production vs. non production conditions) are compared...
  7. pmc A multi-level study of recombinant Pichia pastoris in different oxygen conditions
    Kristin Baumann
    Department of Chemical Engineering, Autonomous University of Barcelona, Spain
    BMC Syst Biol 4:141. 2010
    ..Gene expression profiling was combined with proteomic analyses and the 13C isotope labelling based experimental determination of metabolic fluxes in the central carbon metabolism...
  8. pmc The impact of oxygen on the transcriptome of recombinant S. cerevisiae and P. pastoris - a comparative analysis
    Kristin Baumann
    Department of Chemical Engineering, Autonomous University of Barcelona, Spain
    BMC Genomics 12:218. 2011
    ....
  9. pmc Overexpression of the riboflavin biosynthetic pathway in Pichia pastoris
    Hans Marx
    Institute of Applied Microbiology, Department of Biotechnology, BOKU University of Natural Resources and Applied Life Sciences, Muthgasse 18, 1190, Wien, Austria
    Microb Cell Fact 7:23. 2008
    ..abstract:..
  10. pmc Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris
    Diethard Mattanovich
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria
    Microb Cell Fact 8:29. 2009
    ..abstract:..
  11. pmc Open access to sequence: browsing the Pichia pastoris genome
    Diethard Mattanovich
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria
    Microb Cell Fact 8:53. 2009
    ..pastoris research, so that the Pichia community can benefit from exchange of knowledge, data and materials...
  12. pmc Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview
    Brigitte Gasser
    University of Natural Resources and Applied Life Sciences Vienna, Department of Biotechnology, Vienna, Austria
    Microb Cell Fact 7:11. 2008
    ....
  13. pmc The response to unfolded protein is involved in osmotolerance of Pichia pastoris
    Martin Dragosits
    Department of Biotechnology, BOKU University of Natural Resources and Applied Life Sciences, Vienna, Austria
    BMC Genomics 11:207. 2010
    ..While our main intention was to analyze the effect of different osmolarities on P. pastoris in general, this was complemented by studying it in context with recombinant protein production...
  14. doi request reprint The effect of temperature on the proteome of recombinant Pichia pastoris
    Martin Dragosits
    Department of Biotechnology, BOKU University of Natural Resources and Applied Life Sciences, Vienna, Austria
    J Proteome Res 8:1380-92. 2009
    ..These data indicate that folding stress is generally decreased at lower cultivation temperatures, enabling more efficient heterologous protein secretion in P. pastoris host cells...
  15. pmc Monitoring of transcriptional regulation in Pichia pastoris under protein production conditions
    Brigitte Gasser
    University of Natural Resources and Applied Life Sciences Vienna, Department of Biotechnology, Institute of Applied Microbiology, Vienna, Austria
    BMC Genomics 8:179. 2007
    ....
  16. pmc Transcriptomics-based identification of novel factors enhancing heterologous protein secretion in yeasts
    Brigitte Gasser
    Institute of Applied Microbiology, Department of Biotechnology, University of Natural Resources and Applied Life Sciences Vienna, Muthgasse 18, A 1190 Vienna, Austria
    Appl Environ Microbiol 73:6499-507. 2007
    ..5-fold in fed-batch fermentations of P. pastoris...
  17. pmc Novel insights into the unfolded protein response using Pichia pastoris specific DNA microarrays
    Alexandra Graf
    Institute of Applied Microbiology, Department of Biotechnology, University of Natural Resources and Applied Life Sciences Vienna, Muthgasse 18, 1190 Vienna, Austria
    BMC Genomics 9:390. 2008
    ..pastoris and analyzed the unfolded protein response (UPR) in this yeast species, as compared to S. cerevisiae...
  18. doi request reprint The secretory pathway: exploring yeast diversity
    Marizela Delic
    Department of Biotechnology, University of Natural Resources and Life Sciences BOKU, Vienna, Austria Austrian Centre of Industrial Biotechnology ACIB GmbH, Vienna, Austria
    FEMS Microbiol Rev 37:872-914. 2013
    ..Recent research obviates that even where homologous genes are present, small differences in protein sequence and/or differences in the regulation of gene expression may lead to quite different protein secretion phenotypes...
  19. pmc Induction without methanol: novel regulated promoters enable high-level expression in Pichia pastoris
    Roland Prielhofer
    University of Natural Resources and Life Sciences, Department of Biotechnology, Muthgasse 18, Vienna 1190, Austria
    Microb Cell Fact 12:5. 2013
    ..g. glycerol, while heterologous gene expression should be active in the feed phase under carbon (e.g. glucose) limitation...
  20. doi request reprint Recombinant protein production in yeasts
    Diethard Mattanovich
    Department of Biotechnology, University of Natural Resources and Life Sciences, and Austrian Centre of Industrial Biotechnology, Vienna, Austria
    Methods Mol Biol 824:329-58. 2012
    ....
  21. doi request reprint Pichia pastoris: protein production host and model organism for biomedical research
    Brigitte Gasser
    University of Natural Resources and Life Sciences BOKU, Department of Biotechnology, 1190 Vienna, Austria
    Future Microbiol 8:191-208. 2013
    ..At the same time, it has become obvious that P. pastoris, as an evolutionarily more 'ancient' yeast, may in some cases be a better model for human cell biology and disease than Saccharomyces cerevisiae...
  22. doi request reprint Identification and characterisation of novel Pichia pastoris promoters for heterologous protein production
    Gerhard Stadlmayr
    Department of Biotechnology, BOKU University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria
    J Biotechnol 150:519-29. 2010
    ....
  23. doi request reprint Induction and measurement of UPR and osmotic stress in the yeast Pichia pastoris
    Martin Dragosits
    Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria
    Methods Enzymol 489:165-88. 2011
    ..pastoris directly without the hassle to modify standard protocols designed for the model organism S. cerevisiae first...
  24. ncbi request reprint Oxidative protein folding and unfolded protein response elicit differing redox regulation in endoplasmic reticulum and cytosol of yeast
    Marizela Delic
    Department of Biotechnology, University of Natural Resources and Life Sciences Vienna, 1190 Vienna, Austria
    Free Radic Biol Med 52:2000-12. 2012
    ..These data clearly demonstrate that protein folding and ER stress have a severe impact on the cytosolic redox balance, which may be a major factor during development of folding-related diseases...
  25. ncbi request reprint Engineering of Pichia pastoris for improved production of antibody fragments
    Brigitte Gasser
    Institute of Applied Microbiology, BOKU University of Natural Resources and Applied Life Sciences Vienna, Muthgasse 18, A 1190 Vienna, Austria
    Biotechnol Bioeng 94:353-61. 2006
    ..3-fold, PDI enabled an increase of the Fab level by 1.9-fold. Hence, the formation of interchain disulfide bonds can be seen as a major rate limiting factor to Fab assembly and subsequent secretion...
  26. doi request reprint Genome-scale analysis of library sorting (GALibSo): Isolation of secretion enhancing factors for recombinant protein production in Pichia pastoris
    Gerhard Stadlmayr
    Department of Biotechnology, BOKU University of Natural Resources and Applied Life Sciences, Vienna, Austria
    Biotechnol Bioeng 105:543-55. 2010
    ..The microarray based monitoring of the enrichment of genes causing enhanced protein secretory capacity led to novel insights into the limitation of protein secretion...
  27. ncbi request reprint Cloning, disruption and protein secretory phenotype of the GAS1 homologue of Pichia pastoris
    Hans Marx
    Institute of Applied Microbiology, Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria
    FEMS Microbiol Lett 264:40-7. 2006
    ..pastoris, human trypsinogen, human serum albumin and Rhizopus oryzae lipase, was evaluated. While the disruption had no effect on the secretion of trypsinogen and albumin, the amount of lipase released from the cells was doubled...
  28. ncbi request reprint Interlaboratory comparison for quantitative primary metabolite profiling in Pichia pastoris
    Kristaps Klavins
    Austrian Centre of Industrial Biotechnology, Muthgasse 11, 1190 Vienna, Austria
    Anal Bioanal Chem 405:5159-69. 2013
    ..The application of isotope dilution upon extraction was an absolute prerequisite for the quantification of the redox-sensitive amino acid methionine, where no agreement between the two laboratories could be achieved...
  29. ncbi request reprint Effects of gene dosage, promoters, and substrates on unfolded protein stress of recombinant Pichia pastoris
    Hubertus Hohenblum
    Institute of Applied Microbiology, BOKU University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190, Vienna, Austria
    Biotechnol Bioeng 85:367-75. 2004
    ....
  30. doi request reprint Accurate quantification of the redox-sensitive GSH/GSSG ratios in the yeast Pichia pastoris by HILIC-MS/MS
    Christina Haberhauer-Troyer
    Department of Chemistry, Division of Analytical Chemistry, University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria
    Anal Bioanal Chem 405:2031-9. 2013
    ....
  31. ncbi request reprint U13C cell extract of Pichia pastoris--a powerful tool for evaluation of sample preparation in metabolomics
    Stefan Neubauer
    Department of Chemistry, Division of Analytical Chemistry, University of Natural Resources and Life Sciences BOKU, Vienna, Austria
    J Sep Sci 35:3091-105. 2012
    ..The optimum case would be represented by metabolites, easy to extract from yeast with high and precise recovery. In other cases the total combined uncertainty was significantly higher...
  32. doi request reprint Intracellular interactome of secreted antibody Fab fragment in Pichia pastoris reveals its routes of secretion and degradation
    Martin Pfeffer
    Department of Biotechnology, University of Natural Resources and Life Sciences BOKU, Vienna, Austria
    Appl Microbiol Biotechnol 93:2503-12. 2012
    ..This work will further facilitate our understanding how recombinant proteins behave in the secretory pathway...
  33. doi request reprint Engineering of biotin-prototrophy in Pichia pastoris for robust production processes
    Brigitte Gasser
    Department of Biotechnology, University of Natural Resources and Life Sciences BOKU, Muthgasse 18, 1190 Vienna, Austria
    Metab Eng 12:573-80. 2010
    ..Another field of application for these truly prototrophic hosts is the production of biochemicals and small metabolites, where defined mineral media leads to easier purification procedures...
  34. pmc Improvement of lactic acid production in Saccharomyces cerevisiae by cell sorting for high intracellular pH
    Minoska Valli
    Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
    Appl Environ Microbiol 72:5492-9. 2006
    ..The best lactic-acid-producing strain was identified within the screening of CEN.PK m850 mutants...
  35. ncbi request reprint High level expression of a promising anti-idiotypic antibody fragment vaccine against HIV-1 in Pichia pastoris
    Johannes S Gach
    Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
    J Biotechnol 128:735-46. 2007
    ..Biochemical analysis confirmed that the N-glycosylation does not contribute to the binding properties of 3H6...
  36. ncbi request reprint 16 years research on lactic acid production with yeast - ready for the market?
    Michael Sauer
    School of Bioengineering, University of Applied Sciences FH Campus Wien, Vienna, Austria
    Biotechnol Genet Eng Rev 27:229-56. 2010
    ..Now yeasts are very close to industrial exploitation - here we summarize the developments in this field...
  37. doi request reprint Influence of growth temperature on the production of antibody Fab fragments in different microbes: a host comparative analysis
    Martin Dragosits
    Dept of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria
    Biotechnol Prog 27:38-46. 2011
    ....
  38. doi request reprint Directed gene copy number amplification in Pichia pastoris by vector integration into the ribosomal DNA locus
    Hans Marx
    Department of Biotechnology, BOKU, University of Natural Resources and Applied Life Sciences, Vienna, Austria
    FEMS Yeast Res 9:1260-70. 2009
    ....
  39. doi request reprint Targeting enzymes to the right compartment: metabolic engineering for itaconic acid production by Aspergillus niger
    Marzena L Blumhoff
    Department of Biotechnology, BOKU VIBT University of Natural Resources and Life Sciences, Vienna, Austria School of Bioengineering, University of Applied Sciences FH Campus Wien, Vienna, Austria Electronic address
    Metab Eng 19:26-32. 2013
    ..It is essential to target enzymes to the correct compartment in order to establish a proper flux through a compartmentalized pathway. ..
  40. ncbi request reprint Effect of increased expression of protein disulfide isomerase and heavy chain binding protein on antibody secretion in a recombinant CHO cell line
    Nicole Borth
    Institute for Applied Microbiology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, 1190 Vienna, Austria
    Biotechnol Prog 21:106-11. 2005
    ..Overexpression of BIP, both alone and in combination with PDI, reduced the specific secretion rate, whereas PDI, when overexpressed alone, caused an increase of product secretion rate...
  41. doi request reprint Identification and deletion of the major secreted protein of Pichia pastoris
    Silvia Heiss
    Department of Biotechnology, University of Natural Resources and Life Sciences BOKU, 1190, Vienna, Austria
    Appl Microbiol Biotechnol 97:1241-9. 2013
    ..Batch purification of a Fab fragment produced in the Δepx1 strain highlighted its superior purity due to the decreased host cell protein load...
  42. ncbi request reprint Reverse engineering of protein secretion by uncoupling of cell cycle phases from growth
    Markus Buchetics
    Department of Biotechnology, University of Natural Resources and Life Sciences Vienna, Muthgasse 18, 1190 Vienna, Austria telephone 43476546569 fax 4313697615 Austrian Centre of Industrial Biotechnology ACIB GmbH, Vienna, Austria
    Biotechnol Bioeng 108:2403-12. 2011
    ..This concept was verified with another secreted protein, human trypsinogen. Biotechnol. Bioeng. 2011;108: 2403-2412. © 2011 Wiley Periodicals, Inc. ..
  43. ncbi request reprint In Pichia pastoris, growth rate regulates protein synthesis and secretion, mating and stress response
    Corinna Rebnegger
    Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria
    Biotechnol J 9:511-25. 2014
    ..We conclude that a high μ positively affects specific protein secretion rates by acting on multiple cellular processes. ..
  44. doi request reprint Genetic engineering of Lactobacillus diolivorans
    Stefan Pflügl
    School of Bioengineering, FH Campus Wien University of Applied Sciences, Vienna, Austria
    FEMS Microbiol Lett 344:152-8. 2013
    ..Finally, the homologous overexpression of a putatively NADPH-dependent 1,3-propanediol oxidoreductase improved 1,3-propanediol production by 20% in batch cultures...
  45. pmc Intracellular pH distribution in Saccharomyces cerevisiae cell populations, analyzed by flow cytometry
    Minoska Valli
    Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
    Appl Environ Microbiol 71:1515-21. 2005
    ..Notably, a different behavior was observed for exponentially grown and stationary cells in terms of the appearance and development of these subpopulations as a response to a changing external pH...
  46. doi request reprint Recombinant protein production 6 (Vienna, February 2011)
    Brigitte Gasser
    Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria
    N Biotechnol 30:247-9. 2013
    ..Additionally, the development of novel secretion systems was presented, giving one example of how to combine industry's needs with highly ambitious fundamental research...
  47. doi request reprint Yeast systems biotechnology for the production of heterologous proteins
    Alexandra Graf
    Department of Biotechnology, Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, Vienna, Austria
    FEMS Yeast Res 9:335-48. 2009
    ..The development of next generation sequencing technology enables an unexpected revival of genomic approaches, providing new potential for evolutionary engineering and inverse metabolic engineering...
  48. ncbi request reprint Model based engineering of Pichia pastoris central metabolism enhances recombinant protein production
    Justyna Nocon
    Department of Biotechnology, University of Natural Resources and Life Sciences Vienna, Muthgasse 18, Vienna, Austria
    Metab Eng 24:129-38. 2014
    ..Genome scale metabolic modeling is shown to predict overexpression and deletion mutants which enhance recombinant protein production with high accuracy. ..
  49. doi request reprint 1,3-Propanediol production from glycerol with Lactobacillus diolivorans
    Stefan Pflügl
    School of Bioengineering, FH Campus Wien University of Applied Sciences, Muthgasse 62, 1190 Vienna, Austria
    Bioresour Technol 119:133-40. 2012
    ..Furthermore, the addition of Vitamin B(12) to the culture medium increased production by 15% to a final titer of 84.5 g/l 1,3-propanediol...
  50. doi request reprint Microbial production of organic acids: expanding the markets
    Michael Sauer
    School of Bioengineering, FH Campus Wien University of Applied Sciences, Muthgasse 18, 1190 Wien, Austria
    Trends Biotechnol 26:100-8. 2008
    ..In this article, through analysis of the current advances in production of these acids, we present guidelines for future developments in this fast-moving field...
  51. ncbi request reprint Stress in recombinant protein producing yeasts
    Diethard Mattanovich
    Institute of Applied Microbiology, BOKU University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
    J Biotechnol 113:121-35. 2004
    ..Apart from discussing well-documented stress reactions of yeasts in the context of heterologous protein production, some more speculative topics like quorum sensing and apoptosis are addressed...
  52. doi request reprint Six novel constitutive promoters for metabolic engineering of Aspergillus niger
    Marzena Blumhoff
    School of Bioengineering, University of Applied Sciences FH Campus Wien, Muthgasse 56, 1190, Vienna, Austria
    Appl Microbiol Biotechnol 97:259-67. 2013
    ..Product titers from the detection limit to up to 570 mg/L proved that the set of constitutive promoters is a powerful tool for the fine-tuning of metabolic pathways for the improvement of industrial production processes...
  53. ncbi request reprint Bacterial expression and refolding of human trypsinogen
    Hubertus Hohenblum
    Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, Vienna, Austria
    J Biotechnol 109:3-11. 2004
    ..The refolded trypsinogen was converted to trypsin by autocatalytic activation, and the utility for the detachment of mammalian cells in culture was proven...
  54. ncbi request reprint Microbial production of 1,3-propanediol
    Michael Sauer
    School of Bioengineering, FH Campus Wien University of Applied Sciences, Muthgasse 18, 1190 Vienna, Austria
    Recent Pat Biotechnol 2:191-7. 2008
    ..Similar questions arise for a variety of products if they are to be produced bio-based in large scale. However, special emphasis is given to 1,3-propanediol production...
  55. ncbi request reprint Sample preparation workflow for the liquid chromatography tandem mass spectrometry based analysis of nicotinamide adenine dinucleotide phosphate cofactors in yeast(†)
    Karin Ortmayr
    Department of Chemistry, University of Natural Resources and Life Sciences Vienna, Vienna, Austria
    J Sep Sci 37:2185-91. 2014
    ..Interestingly, a difference was also observed in the nicotinamide adenine dinucleotide phosphate pool size, which was significantly higher in the wildtype than in the modified strain. ..
  56. ncbi request reprint Pichia pastoris secretes recombinant proteins less efficiently than Chinese hamster ovary cells but allows higher space-time yields for less complex proteins
    Andreas Maccani
    Austrian Centre of Industrial Biotechnology ACIB GmbH, Vienna, Austria Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria
    Biotechnol J 9:526-37. 2014
    ..pastoris is much less efficient and the secretion rate strongly depends on the complexity of the recombinant protein. However, process efficiency of the yeast system allows higher STYs for less complex proteins. ..
  57. doi request reprint Monitoring intracellular redox conditions in the endoplasmic reticulum of living yeasts
    Marizela Delic
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences BOKU, Vienna, Austria
    FEMS Microbiol Lett 306:61-6. 2010
    ..Contrary to previous results with roGFP, the optimized roGFP1_iE and roGFP1_iL constructs were not completely oxidized, and are therefore useful sensors for monitoring the ER under conditions when it is even more oxidized...
  58. pmc Modeling and measuring intracellular fluxes of secreted recombinant protein in Pichia pastoris with a novel 34S labeling procedure
    Martin Pfeffer
    University of Natural Resources and Life Sciences, Department of Biotechnology, Muthgasse 18, Vienna, Austria
    Microb Cell Fact 10:47. 2011
    ..Thus labeling procedures must be compatible with minimal production media and the usage of bioreactors. The inorganic and non-radioactive 34S labeled sodium sulfate meets both demands...
  59. ncbi request reprint Antibody production with yeasts and filamentous fungi: on the road to large scale?
    Brigitte Gasser
    Institute of Applied Microbiology, Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria
    Biotechnol Lett 29:201-12. 2007
    ..Following aspects are addressed: folding, assembly and secretion of antibody related proteins, process optimization to improve productivity and quality, proteolysis, and, as a major point of interest, glycosylation...
  60. doi request reprint Heading for an economic industrial upgrading of crude glycerol from biodiesel production to 1,3-propanediol by Lactobacillus diolivorans
    Stefan Pflügl
    School of Bioengineering, FH Campus Wien University of Applied Sciences, Muthgasse 62, 1190 Vienna, Austria Department of Biotechnology, BOKU VIBT University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria
    Bioresour Technol 152:499-504. 2014
    ..7 and 0.3 g/l, respectively. Addition of furfural and 5-hydroxymethylfurfural to batch cultures in said concentrations did not show inhibitory effects on growth or 1,3-propanediol production...
  61. pmc From rumen to industry
    Michael Sauer
    Department of Biotechnology, BOKU VIBT University of Natural Resources and Life Sciences, Muthgasse 18, Vienna 1190, Austria
    Microb Cell Fact 11:121. 2012
    ..We suggest to intensify the studies of the ruminal microbial ecosystem from an industrial microbiologists point of view in order to make use of this rich source of organisms and enzymes...
  62. ncbi request reprint Mass spectrometry based analysis of nucleotides, nucleosides, and nucleobases--application to feed supplements
    Stefan Neubauer
    Department of Chemistry, Division of Analytical Chemistry, University of Natural Resources and Life Sciences BOKU, Vienna, Austria
    Anal Bioanal Chem 404:799-808. 2012
    ..Sensitivity of LC-ICP-MS was 1-2 orders of magnitude lower than that of LC-MS-MS. Quantitative analysis of yeast products using complementary MS detection furnished values in good agreement...
  63. ncbi request reprint Engineering of protein folding and secretion-strategies to overcome bottlenecks for efficient production of recombinant proteins
    Marizela Delic
    1 Department of Biotechnology, University of Natural Resources and Life Sciences BOKU, Vienna, Austria
    Antioxid Redox Signal 21:414-37. 2014
    ..In addition, systems biology-based whole cell analysis that also takes energy and redox metabolism into consideration will broaden the knowledge base for future rational engineering strategies. Antioxid. Redox Signal. 21, 414-437. ..
  64. pmc Adaptive laboratory evolution -- principles and applications for biotechnology
    Martin Dragosits
    Department of Chemistry, University of Natural Resources and Life Sciences, Muthgasse 11, A 1190 Vienna, Austria
    Microb Cell Fact 12:64. 2013
    ..Altogether the current status and its future perspectives highlight the importance and potential of adaptive laboratory evolution as approach in biotechnological engineering. ..
  65. pmc Biochemistry of microbial itaconic acid production
    Matthias G Steiger
    Austrian Centre of Industrial Biotechnology ACIB GmbH Vienna, Austria Department of Biotechnology, BOKU Vienna Institute of BioTechnology, University of Natural Resources and Life Sciences Vienna, Austria
    Front Microbiol 4:23. 2013
    ..This review will describe the current status and recent advances in the understanding of the molecular processes leading to the biotechnological production of itaconic acid...
  66. pmc Genome sequence of the ruminal bacterium Megasphaera elsdenii
    Hans Marx
    Department of Biotechnology, BOKU University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria
    J Bacteriol 193:5578-9. 2011
    ..Furthermore, it is of biotechnological interest due to its capability of producing various volatile fatty acids. Here we report the complete genome sequence of M. elsdenii DSM 20460, the type strain for the species...
  67. ncbi request reprint Assessing viability and cell-associated product of recombinant protein producing Pichia pastoris with flow cytometry
    Hubertus Hohenblum
    Institute of Applied Microbiology, BOKU University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
    J Biotechnol 102:281-90. 2003
    ..pastoris...
  68. ncbi request reprint Optimization of recombinant protein expression level in Escherichia coli by flow cytometry and cell sorting
    Elena Soriano
    Institute of Applied Microbiology, University of Agricultural Sciences, Muthgasse 18, A 1190 Vienna, Austria
    Biotechnol Bioeng 80:93-9. 2002
    ..The clones obtained had a similar SOD production compared to the original strain, but were able to reach higher densities in a batch culture, which resulted in a higher total yield...