Gert Lubec

Summary

Affiliation: University of Vienna
Country: Austria

Publications

  1. ncbi Proteomic characterization of the human cortical neuronal cell line HCN-2
    Andreas Peyrl
    Department of Pediatrics, University of Vienna, Waehringer Guertel 18 20, A 1090 Vienna, Austria
    J Chem Neuroanat 26:171-8. 2003
  2. ncbi Increased expression of human reduced folate carrier in fetal Down syndrome brain
    G Lubec
    Department of Pediatrics, University of Vienna, Vienna, Austria
    J Neural Transm Suppl . 2003
  3. ncbi Manifold reduction of moesin in fetal Down syndrome brain
    B Lubec
    Department of Neonatology, University of Vienna, Vienna, A 1090, Austria
    Biochem Biophys Res Commun 286:1191-4. 2001
  4. ncbi Proteomics in brain research: potentials and limitations
    Gert Lubec
    Department of Pediatrics, University of Vienna, Wahringer Gurtel 18, A 1090, Vienna, Austria
    Prog Neurobiol 69:193-211. 2003
  5. ncbi The brain in Down syndrome (TRISOMY 21)
    Gert Lubec
    Department of Pediatrics, University of Vienna, Wahringer Gurtel 18 20, 1090 Vienna, Austria
    J Neurol 249:1347-56. 2002
  6. doi Linkage of hippocampal proteins to spatial memory formation and strain-dependence in Apodemus sylvaticus, C57BL/6J and PWD/PhJ mice
    Sudarshan S Patil
    Department of Pediatrics, Division of Pediatric Neuroscience, Medical University of Vienna, Wahringer Gurtel 18 20, A 1090 Vienna, Austria
    Neurochem Int 56:522-7. 2010
  7. doi Mitosis-dependent protein expression in neuroblastoma cell line N1E-115
    Amedeo A Azizi
    Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, Wahringer Gurtel 18 20, Vienna, Austria
    J Proteome Res 7:3412-22. 2008
  8. doi Mice transgenic for reduced folate carrier: an animal model of Down syndrome?
    Joachim Höger
    Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, Waehringer Guertel 18, 1090 Vienna, Austria
    Amino Acids 36:349-57. 2009
  9. doi Hippocampal levels of phosphorylated protein kinase A (phosphor-S96) are linked to spatial memory enhancement by SGS742
    Berta Sunyer
    Department of Pediatrics, Medical University of Vienna, Vienna, Austria
    Hippocampus 19:90-8. 2009
  10. ncbi Proteome analysis in hippocampus of mice overexpressing human Cu/Zn-superoxide dismutase 1
    Joo Ho Shin
    Department of Pediatrics, Medical University of Vienna, Wahringer Gurtel 18 20, 1090 Vienna, Austria
    Neurochem Int 46:641-53. 2005

Detail Information

Publications111 found, 100 shown here

  1. ncbi Proteomic characterization of the human cortical neuronal cell line HCN-2
    Andreas Peyrl
    Department of Pediatrics, University of Vienna, Waehringer Guertel 18 20, A 1090 Vienna, Austria
    J Chem Neuroanat 26:171-8. 2003
    ..We could prove the existence of these 14 proteins in the human neuron, extending the list of proteins with neuronal expression and identify possible new candidate marker proteins...
  2. ncbi Increased expression of human reduced folate carrier in fetal Down syndrome brain
    G Lubec
    Department of Pediatrics, University of Vienna, Vienna, Austria
    J Neural Transm Suppl . 2003
    ....
  3. ncbi Manifold reduction of moesin in fetal Down syndrome brain
    B Lubec
    Department of Neonatology, University of Vienna, Vienna, A 1090, Austria
    Biochem Biophys Res Commun 286:1191-4. 2001
    ..Furthermore, decreased moesin is the second F-actin bundling protein, besides drebrin, that is manifold reduced in fetal DS brain...
  4. ncbi Proteomics in brain research: potentials and limitations
    Gert Lubec
    Department of Pediatrics, University of Vienna, Wahringer Gurtel 18, A 1090, Vienna, Austria
    Prog Neurobiol 69:193-211. 2003
    ..Several other analytical problems need to be overcome, but once the latter problem can be solved, there is nothing to stop the method for a large scale analysis of membrane proteins in neuroscience...
  5. ncbi The brain in Down syndrome (TRISOMY 21)
    Gert Lubec
    Department of Pediatrics, University of Vienna, Wahringer Gurtel 18 20, 1090 Vienna, Austria
    J Neurol 249:1347-56. 2002
    ..Therapeutic modalities that target these factors including antioxidants and caspase inhibitors might have some benefit in alleviating the symptoms of DS...
  6. doi Linkage of hippocampal proteins to spatial memory formation and strain-dependence in Apodemus sylvaticus, C57BL/6J and PWD/PhJ mice
    Sudarshan S Patil
    Department of Pediatrics, Division of Pediatric Neuroscience, Medical University of Vienna, Wahringer Gurtel 18 20, A 1090 Vienna, Austria
    Neurochem Int 56:522-7. 2010
    ..The finding that the neuronal protein NeuN is able to discriminate between good and bad performers in the MWM may be of major interest and may open a new area in the search for protein markers of spatial memory performance...
  7. doi Mitosis-dependent protein expression in neuroblastoma cell line N1E-115
    Amedeo A Azizi
    Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, Wahringer Gurtel 18 20, Vienna, Austria
    J Proteome Res 7:3412-22. 2008
    ..Identification of mitosis-dependent proteins is of importance for the interpretation of previous work and for designing future experiments...
  8. doi Mice transgenic for reduced folate carrier: an animal model of Down syndrome?
    Joachim Höger
    Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, Waehringer Guertel 18, 1090 Vienna, Austria
    Amino Acids 36:349-57. 2009
    ..Increased crossings in the OF may indicate modulated anxiety-related behavior observed in patients with DS...
  9. doi Hippocampal levels of phosphorylated protein kinase A (phosphor-S96) are linked to spatial memory enhancement by SGS742
    Berta Sunyer
    Department of Pediatrics, Medical University of Vienna, Vienna, Austria
    Hippocampus 19:90-8. 2009
    ..The finding that somatostatin-14 was also induced in the SGS742-treated yoked controls points to a drug side effect, and therefore the role of somatostatin-14 for cognitive enhancement remains open...
  10. ncbi Proteome analysis in hippocampus of mice overexpressing human Cu/Zn-superoxide dismutase 1
    Joo Ho Shin
    Department of Pediatrics, Medical University of Vienna, Wahringer Gurtel 18 20, 1090 Vienna, Austria
    Neurochem Int 46:641-53. 2005
    ..Our findings suggest that overexpressed SOD1 directly or by generating reactive oxygen species may lead to aberrant protein expressional patterns that in turn may lead to or reflect neurodegeneration observed in this animal model...
  11. ncbi Proteins involved in neuronal differentiation of neuroblastoma cell line N1E-115
    Ji Eun Oh
    Department of Pediatrics, Medical University of Vienna, Vienna, Austria
    Electrophoresis 28:2009-17. 2007
    ..The study forms the molecular basis for further evaluation of the functional roles of the differentially expressed proteins in ND...
  12. doi Distinct set of kinases induced after retrieval of spatial memory discriminate memory modulation processes in the mouse hippocampus
    Lin Li
    Department of Pediatrics, Medical University of Vienna, Vienna, Austria
    Hippocampus 23:672-83. 2013
    ..Together with its recently observed genetic association to autism-spectrum disorders our data suggest a role of this kinase in balancing preservation and flexibility of learned behavior...
  13. ncbi Strain-dependent regulation of plasticity-related proteins in the mouse hippocampus
    Daniela D Pollak
    Department of Pediatrics, Division of Pediatric Neuroscience, Medical University of Vienna, Waehringer Guertel 18 20, A 1090 Vienna, Austria
    Behav Brain Res 165:240-6. 2005
    ..Data presented herein highlight the importance of careful selection of the mouse strain for studies of synaptic plasticity...
  14. ncbi Protein chemical identification and characterization of the human variants of far upstream element binding protein in medulloblastoma DAOY cell line
    Ephrem Engidawork
    Department of Pediatrics, Medical University of Vienna, A 1090, Vienna, Austria
    Int J Oncol 29:721-36. 2006
    ..These findings open up an exciting possibility for discerning the cellular roles of FAB in tumor biology...
  15. ncbi Fluctuations of hippocampal neuronal protein levels over the estrous cycle in the rat
    Wei Fei Diao
    Department of Pediatrics, Medical University of Vienna, Waehringer Guertel 18, A 1090 Vienna, Austria
    Neurochem Int 52:1002-11. 2008
    ....
  16. ncbi Strain-independent global effect of hippocampal proteins in mice trained in the Morris water maze
    Kongzhao Li
    Department of Pediatrics, Medical University of Vienna, Wahringer Gurtel 18 20, 1090, Vienna, Austria
    Amino Acids 43:1739-49. 2012
    ..Four proteins verified by immunoblotting could be shown to be involved in training in the MWM as a global effect, independent of the strain tested...
  17. doi Blocking mTORC1 activity by rapamycin leads to impairment of spatial memory retrieval but not acquisition in C57BL/6J mice
    Alev Deli
    Department of Pediatrics, Medical University of Vienna, Wahringer Gurtel 18, 1090 Vienna, Austria
    Behav Brain Res 229:320-4. 2012
    ..The current study extends knowledge on rapamycin in memory mechanisms and challenges work on deeper insights into the role of mTOR in different phases of memory formation and retrieval...
  18. doi Cognitive enhancement by SGS742 in OF1 mice is linked to specific hippocampal protein expression
    Berta Sunyer
    Department of Pediatrics, Medical University of Vienna, Vienna, Austria
    J Proteome Res 7:5237-53. 2008
    ..Significantly different protein spots were identified by two mass spectrometry principles, nano-LC-ESI-MS/MS and by nano-LC-ESI(CID/ETD)-MS/MS. Signaling, chaperone and metabolic enzyme proteins were linked to memory enhancement...
  19. ncbi Impaired cognitive performance in neuronal nitric oxide synthase knockout mice is associated with hippocampal protein derangements
    Liselotte Kirchner
    Department of Neonatology, University of Vienna, Wahringer Gurtel 18, A 1090 Vienna, Austria
    Nitric Oxide 11:316-30. 2004
    ..We conclude that nNOS knockout mice show impaired spatial performance in the MWM, a finding that may be either linked to direct effects of nNOS/NO and/or to specific hippocampal protein derangements...
  20. doi Complete sequencing of GABAA receptor subunit beta 3 by a rapid technique following in-gel digestion of the protein
    Sung Ung Kang
    Department of Pediatrics, Medical University of Vienna, Vienna, Austria
    Electrophoresis 30:2159-67. 2009
    ..It was observed that the use of trypsin, chymotrypsin and Asp-N with bioinformatic handling by the MASCOT and Modiro software is sufficient to obtain complete sequencing of a highly hydrophobic membrane protein...
  21. doi Differential protein levels and post-translational modifications in spinal cord injury of the rat
    Leila Afjehi-Sadat
    Department of Pediatrics, Medical University of Vienna, Vienna, Austria
    J Proteome Res 9:1591-7. 2010
    ..The findings propose the involvement of the corresponding cascades and challenge further work into aberrant signaling and oxidative stress in SCI, which may form the basis for experimental intervention for spinal cord trauma...
  22. doi Serine/threonine-protein phosphatase 1 α levels are paralleling olfactory memory formation in the CD1 mouse
    Christiana Winding
    Division of Neuroproteomics, Department of Pediatrics, Medical University of Vienna, Vienna, Austria
    Electrophoresis 32:1675-83. 2011
    ..PP-1A is a key signalling element in synaptic plasticity and memory processes and is herein shown to be paralleling olfactory discrimination representing olfactory memory...
  23. doi Strain-dependent hippocampal protein levels of GABA(B)-receptor subunit 2 and NMDA-receptor subunit 1
    Berta Sunyer
    Department of Pediatrics, Medical University of Vienna, Wahringer Gurtel 18 20, A 1090 Vienna, Austria
    Neurochem Int 55:253-6. 2009
    ..Modification of receptor complex stoichiometry in the hippocampus in turn may well be responsible for cognitive differences observed in spatial memory between mouse strains...
  24. doi Evaluation of spatial memory of C57BL/6J and CD1 mice in the Barnes maze, the Multiple T-maze and in the Morris water maze
    Sudarshan S Patil
    Department of Pediatrics, Medical University of Vienna, Waehringer Guertel 18 20, A 1090 Vienna, Austria
    Behav Brain Res 198:58-68. 2009
    ....
  25. doi Neurotrophin 3/TrkC-regulated proteins in the human medulloblastoma cell line DAOY
    Mariella Gruber-Olipitz
    Department of Pediatrics, Medical University of Vienna, Vienna, Austria
    Electrophoresis 30:540-9. 2009
    ..Knowledge on effectors of TrkC signaling may represent a first useful step for the identification of marker candidates or reflecting probable pharmacological targets for specific treatment of MB...
  26. ncbi p27Kip1 localization depends on the tumor suppressor protein tuberin
    Margit Rosner
    Medical Genetics, Obstetrics and Gynecology, Medical University of Vienna, Wahringer Gurtel 18 20, 1090 Vienna, Austria
    Hum Mol Genet 16:1541-56. 2007
    ..These findings demonstrate p27 localization during the mammalian cell cycle to be under the control of the tumor suppressor tuberin...
  27. ncbi Mass spectrometrical analysis of the mitochondrial carrier Aralar1 from mouse hippocampus
    Seok Heo
    Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, Vienna, Austria
    Electrophoresis 31:1813-21. 2010
    ....
  28. doi Mass spectrometrical characterisation of mouse and rat synapsin isoforms 2a and 2b
    Sung Ung Kang
    Department of Pediatrics, Medical University of Vienna, Waehringer Guertel 18, 1090, Vienna, Austria
    Amino Acids 38:1131-43. 2010
    ..These findings are important for interpretation of previous results and design of future studies on synapsins...
  29. doi Proteins linked to extinction in contextual fear conditioning in the C57BL/6J mouse
    Lin Li
    Department of Pediatrics, Medical University of Vienna, Vienna, Austria
    Proteomics 11:3706-24. 2011
    ..Moreover, it is intriguing to demonstrate the differences between good and poor extinction performance at the protein level...
  30. ncbi Tuberin--a new molecular target in Alzheimer's disease?
    Rosa Ferrando-Miguel
    Medical Genetics, Obstetrics and Gynecology, Medical University of Vienna, General Hospital, 1090, Wahringer Gurtel 18 20, Vienna, 1090, Austria
    Neurochem Res 30:1413-9. 2005
    ..This first connection of tuberin to another common disease beside TSC stimulates new approaches to investigate the molecular development and to establish new therapeutic strategies...
  31. ncbi Bach2 is involved in neuronal differentiation of N1E-115 neuroblastoma cells
    Ki Shuk Shim
    Medical Genetics, Obstetrics and Gynecology, Medical University of Vienna, Wahringer Gurtel 18 20, A 1090 Vienna, Austria
    Exp Cell Res 312:2264-78. 2006
    ..It would have negative effects on proliferation and differentiation of these cells. In differentiated neuronal cells Bach2 expression is upregulated, which could allow Bach2 to function as a gatekeeper of the differentiated status...
  32. ncbi Large-scale identification of cytosolic mouse brain proteins by chromatographic prefractionation
    Joo Ho Shin
    Department of Pediatrics, Medical University of Vienna, Austria
    Electrophoresis 27:2799-813. 2006
    ..The results represent so far the largest database of mouse brain proteins and provide valuable information for the design of proteomic studies in the mouse...
  33. doi Hippocampal protein levels related to spatial memory are different in the Barnes maze and in the multiple T-maze
    Jun Fang Zheng
    Department of Pediatrics, Medical University of Vienna, Vienna, Austria
    J Proteome Res 8:4479-86. 2009
    ..Protein levels were linked to spatial memory specifically as yoked controls were used...
  34. ncbi Hippocampal synapsin isoform levels are linked to spatial memory enhancement by SGS742
    Julius Paul Pradeep John
    Department of Pediatrics, Medical University of Vienna, Vienna, Austria
    Hippocampus 19:731-8. 2009
    ....
  35. doi Complete sequencing of the recombinant granulocyte-colony stimulating factor (filgrastim) and detection of biotinylation by mass spectrometry
    Kareem Eldin A M Ahmed
    Department of Pediatrics, Medical University of Vienna, Waehringer Guertel 18, 1090, Vienna, Austria
    Amino Acids 38:1043-9. 2010
    ..Although there is some evidence that biotinylated G-CSF analogues are active, it remains open whether this modification may be responsible for the side effects observed or lead to changes of antigenicity...
  36. ncbi Protein dysregulation in mouse hippocampus polytransgenic for chromosome 21 structures in the Down Syndrome Critical Region
    Joo Ho Shin
    Department of Pediatrics, Division of Neuroproteomics, Medical University of Vienna, Austria
    J Proteome Res 5:44-53. 2006
    ....
  37. doi Changes of hippocampal signaling protein levels during postnatal brain development in the rat
    Rachel Weitzdörfer
    Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, Vienna, Austria
    Hippocampus 18:807-13. 2008
    ..These data contribute to the understanding that different time points may use different signaling cascades...
  38. ncbi Dysregulation of growth factor receptor-bound protein 2 and fascin in hippocampus of mice polytransgenic for chromosome 21 structures
    Joo Ho Shin
    Department of Pediatrics, Medical University of Vienna, Vienna, Austria
    Hippocampus 17:1180-92. 2007
    ..Fascin dysregulation is of relevance for actin bundling in vesicle trafficking and may represent or lead to impaired neurotransmission that, in turn, may lead to the cognitive defect observed in this mouse model of Down syndrome...
  39. ncbi Derangement of hypothetical proteins in fetal Down's syndrome brain
    Joo Ho Shin
    Department of Pediatrics, University of Vienna, Vienna, Austria
    Neurochem Res 29:1307-16. 2004
    ..05) in fetal DS brain, and domain analysis suggests involvement in cytoskeleton, signaling, and chaperone system abnormalities...
  40. doi Modulation of the hippocampal protein machinery in voluntary and treadmill exercising rats
    Wei Qiang Chen
    Medical University of Vienna, Department of Pediatrics, Waehringer Guertel 18, A 1090 Vienna, Austria
    Biochim Biophys Acta 1784:555-62. 2008
    ..005 was considered as statistically significant thus respecting multiple testing. The biological meaning of changed protein levels in hippocampus under different conditions of exercise is not known but warrants further investigation...
  41. ncbi Protein levels of alpha1-tubulin, protein disulfide isomerase, tropomyosins and vimentin are regulated by the tuberous sclerosis gene products
    Markus Hengstschlager
    Obstetrics and Gynecology, University of Vienna, Prenatal Diagnosis and Therapy, Wahringer Gurtel 18 20, A 1090 Vienna, Austria
    Cancer Lett 210:219-26. 2004
    ....
  42. ncbi Hippocampal signaling cascades are modulated in voluntary and treadmill exercise rats
    Wei Qiang Chen
    Department of Pediatrics, Medical University of Vienna, Vienna, Austria
    Electrophoresis 28:4392-400. 2007
    ..These results form the basis for functional studies elucidating mechanisms and links between exercise and hippocampal signaling and function...
  43. doi Alzheimer's disease risk factor lymphocyte-specific protein tyrosine kinase regulates long-term synaptic strengthening, spatial learning and memory
    Eun Jung Kim
    Department of Neurophysiology and Neuropharmacology, Center for Physiology and Pharmacology, Medical University of Vienna, Schwarzspanierstrasse 17, I, 1090, Vienna, Austria
    Cell Mol Life Sci 70:743-59. 2013
    ..These data provide the basis for the functional characterization of brain Lck, describing the importance of Lck as a critical regulator of both neuronal morphology and in vivo long-term memory...
  44. doi Mass spectrometrical analysis of bilin-binding protein from the wing of Hebomoia glaucippe (Linnaeus, 1758) (Lepidoptera: Pieridae)
    Narkhyun Bae
    Department of Pediatrics, Medical University of Vienna, Vienna, Austria
    Electrophoresis 33:1787-94. 2012
    ..The role of the PTMs remains elusive but nitration, in analogy to nitration effects reported in literature, proposes a role for mechanoelastic proteins and protein-protein interactions...
  45. doi Proteins linked to spatial memory formation of CD1 mice in the multiple T-maze
    Sudarshan S Patil
    Department of Pediatrics, Medical University of Vienna, Wahringer Gurtel 18, 1090 Vienna, Austria
    Hippocampus 22:1075-86. 2012
    ..Differential protein expression in trained vs. yoked mice and in good and no-performers may allow insight into spatial memory formation as well as represent tentative pharmacological targets...
  46. doi Complete sequencing and oxidative modification of manganese superoxide dismutase in medulloblastoma cells
    Julius Paul Pradeep John
    Department of Pediatrics, Medical University of Vienna, Vienna, Austria
    Electrophoresis 30:3006-16. 2009
    ..The findings of different PTMs in the same gel represent Mn-SOD oxidative states, while oxidative modification of His30 and 31 may even reflect decreased Mn-SOD activity...
  47. ncbi Life-long effects of perinatal asphyxia on stress-induced proteins and dynamin 1 in rat brain
    Erwin Kitzmueller
    Department of Pediatrics, University of Vienna, Vienna, Austria
    Neurochem Res 29:1767-77. 2004
    ..Decreased dynamin-1 levels may be considered as a long-term effect on the exocytotic system possibly reflecting or leading to impaired neuronal transport and vesicle-trafficking in PA of the rat of advanced age...
  48. ncbi Mass spectrometric identification of serine hydrolase OVCA2 in the medulloblastoma cell line DAOY
    Amedeo A Azizi
    Department of Pediatrics, Medical University of Vienna, Währinger Gürtel 19 21, A 1090 Vienna, Austria
    Cancer Lett 241:235-49. 2006
    ..In addition to the presence of OVCA2 in medulloblastoma, it was furthermore detectable in three out of 10 human tumour cell-lines as a high abundance protein probably suggesting a role in the tumour biology...
  49. ncbi Tuberous sclerosis genes regulate cellular 14-3-3 protein levels
    Markus Hengstschlager
    Obstetrics and Gynecology, University of Vienna, Prenatal Diagnosis and Therapy, Wahringer Gurtel 18 20, Vienna A 1090, Austria
    Biochem Biophys Res Commun 312:676-83. 2003
    ..These data provide important new insights into the molecular development of this disease especially since both, the TSC genes and the 14-3-3 proteins, are known to be involved in mammalian cell cycle control...
  50. ncbi Hippocampal signaling protein levels are different in early and late metestrus in the rat
    Wei Fei Diao
    Department of Pediatrics, Medical University of Vienna, Waehringer Guertel 18, A 1090, Vienna, Austria
    Biochim Biophys Acta 1774:1044-51. 2007
    ..Moreover, signaling differences in hippocampus are challenging cognitive studies during these two metestrus phases probably revealing cognitive differences between early and late metestrus...
  51. ncbi Mass spectrometric studies on mouse hippocampal synapsins Ia, IIa, and IIb and identification of a novel phosphorylation site at serine-546
    Julius Paul Pradeep John
    Department of Pediatrics, Medical University of Vienna, Waehringer Guertel 18, 1090 Vienna, Austria
    J Proteome Res 6:2695-710. 2007
    ..Several serine phosphorylation sites were confirmed, and a novel phosphorylation site was observed at Ser-546 in synapsin IIa in all gels analyzed...
  52. ncbi Molecular diversity of rat brain proteins as revealed by proteomic analysis
    Jae Won Yang
    Department of Pediatrics, University of Vienna, Vienna, Austria
    Mol Divers 9:385-96. 2005
    ....
  53. doi Hippocampal levels and activity of the sodium/potassium transporting ATPase subunit α-3 (AT1A3) are paralleling memory training in the multiple T-maze in the C57BL/6J mouse
    Seok Heo
    Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, Wahringer Gurtel 18, A 1090 Vienna, Austria
    Neurochem Int 61:702-12. 2012
    ..Herein, we provide evidence for a possible role of AT1A3 in memory mechanisms and support previous findings using different animal models for memory formation...
  54. doi Focal adhesion kinase regulates neuronal growth, synaptic plasticity and hippocampus-dependent spatial learning and memory
    Francisco J Monje
    Department of Neurophysiology and Neuropharmacology, Center for Physiology and Pharmacology, Vienna, Austria
    Neurosignals 20:1-14. 2012
    ..Our data indicate that FAK has a growth-promoting effect, is importantly involved in the regulation of the synaptic function and mediates in vivo hippocampus-dependent spatial learning and memory...
  55. doi A serotonin receptor 1A containing complex in hippocampus of PWD/PhJ mice is linked to training effects in the Barnes maze
    Seok Heo
    Department of Pediatrics, Medical University of Vienna, Wahringer Gurtel 18 20, A 1090 Vienna, Austria
    Behav Brain Res 216:389-95. 2011
    ..This observation shows that a specific complex rather than a receptor subunit as previously shown is involved in memory process and the receptor protein was characterized...
  56. doi Mass spectrometrical identification of hippocampal NMDA receptor subunits NR1, NR2A-D and five novel phosphorylation sites on NR2A and NR2B
    Maryam Ghafari
    Department of Pediatrics, Medical University of Vienna, Währinger Gürtel 18 1090 Vienna, Austria
    J Proteome Res 11:1891-6. 2012
    ..Herein, findings of novel phosphorylation sites are extending knowledge on chemical characterization of the NMDA-R and warrant studying function of site-specific receptor phosphorylation in health and disease...
  57. doi Differences in hippocampal protein levels between C57Bl/6J, PWD/PhJ, and Apodemus sylvaticus are paralleled by differences in spatial memory
    Sudarshan S Patil
    Department of Pediatrics, Medical University of Vienna, Austria
    Hippocampus 21:714-23. 2011
    ..The experimental approach, however, does not allow discriminating between differences in protein levels a priori and different protein levels induced by the MWM testing. © 2010 Wiley-Liss, Inc...
  58. ncbi Extravasation of plasma proteins can confound interpretation of proteomic studies of brain: a lesson from apo A-I in mesial temporal lobe epilepsy
    Jae Won Yang
    Department of Pediatrics, Medical University of Vienna, Waehringer Guertel 18, A 1090 Vienna, Austria
    Brain Res Mol Brain Res 139:348-56. 2005
    ..Care has to be taken when protein expressional findings are to be interpreted in the presence of plasma proteins, including apo A-I, thus clearly representing a confounding factor...
  59. pmc Myelin-mediated inhibition of oligodendrocyte precursor differentiation can be overcome by pharmacological modulation of Fyn-RhoA and protein kinase C signalling
    Alexandra S Baer
    Department of Neurosurgery, Medical University Vienna, Vienna, Austria
    Brain 132:465-81. 2009
    ....
  60. ncbi Behavioral testing upregulates pCaMKII, BDNF, PSD-95 and egr-1 in hippocampus of FVB/N mice
    Daniela D Pollak
    Department of Pediatrics, Division of Pediatric Neuroscience, Medical University of Vienna, Waehringer Guertel 18 20, A 1090 Vienna, Austria
    Behav Brain Res 163:128-35. 2005
    ..Detection of significantly increased pCaMKII, BDNF, PSD-95 and egr-1 induced by behavioral training at the protein level per se is intriguing and supports the proposed importance of these molecules for neuronal information storage...
  61. doi The cognitive enhancer SGS742 does not involve major known signaling cascades in OF1 mice
    Berta Sunyer
    Department of Pediatrics, Medical University of Vienna, Wahringer Gurtel 18 20, Vienna 1090, Austria
    Neurochem Res 33:1384-92. 2008
    ....
  62. doi Synthesis, chaperoning, and metabolism of proteins are regulated by NT-3/TrkC signaling in the medulloblastoma cell line DAOY
    Mariella Gruber-Olipitz
    Department of Pediatrics, Medical University of Vienna, Vienna, Austria
    J Proteome Res 7:1932-44. 2008
    ..Protein levels of translational, splicing, processing, chaperone, protein handling, and metabolism machineries were shown to depend on neurotrophin-3-induced TrkC activation probably representing pharmacological targets...
  63. doi Inhibition of oligodendrocyte precursor cell differentiation by myelin-associated proteins
    Yasir A Syed
    Department of Pediatrics, Medical University of Vienna, Austria
    Neurosurg Focus 24:E5. 2008
    ..The aim of the present study was to characterize the nature of the inhibitory molecules present in myelin...
  64. ncbi Mass spectrometrical characterization of NDRG2 protein (N-myc-downstream regulated gene 2) and description of two novel phosphorylation sites
    Wei Qiang Chen
    Department of Pediatrics, Medical University of Vienna, Waehringer Guertel 18, 1090 Vienna, Austria
    Neurochem Res 32:1969-77. 2007
    ....
  65. pmc Serotonin(1A)-receptor-dependent signaling proteins in mouse hippocampus
    Lin Li
    Department of Pediatrics, Medical University of Vienna, Wahringer Gurtel 18 20, A 1090 Vienna, Austria
    Neuropharmacology 57:556-66. 2009
    ..Reduced SP levels along with findings of two novel phosphorylation sites may be relevant for interpretation of previous and the design of future studies on this receptor system...
  66. doi Contribution of human amniotic fluid stem cells to renal tissue formation depends on mTOR
    Nicol Siegel
    Institute of Medical Genetics, Medical University of Vienna, Währinger Strasse 10, 1090 Vienna, Austria
    Hum Mol Genet 19:3320-31. 2010
    ....
  67. ncbi Cerebellar protein expression in three different mouse strains and their relevance for motor performance
    Daniela Pollak
    Department of Pediatrics, Division of Basic Science, Medical University of Vienna, Wahringer Gurtel 18, A 1090 Vienna, Austria
    Neurochem Int 46:19-29. 2005
    ....
  68. doi A gel-based proteomic method reveals several protein pathway abnormalities in fetal Down syndrome brain
    Yanwei Sun
    Department of Pediatrics, Neuroproteomics Division, Medical University of Vienna, Vienna, Austria
    J Proteomics 74:547-57. 2011
    ..Taken together, aberrant protein levels from several pathways in fetal DS are challenging as well as fertilizing the area of research and providing the basis for additional neurochemical and functional studies...
  69. ncbi Involvement of individual hippocampal signaling protein levels in spatial memory formation is strain-dependent
    Sudarshan S Patil
    Division of Pediatric Neuroscience, Department of Pediatrics, Medical University of Vienna, Wahringer Gurtel 18 20, 1090, Vienna, Austria
    Amino Acids 39:75-87. 2010
    ..It is intriguing that individual mouse strains, laboratory or wild caught, may use different signaling pathways for spatial memory in the Morris water maze...
  70. ncbi Estrous-cycle-dependent hippocampal levels of signaling proteins
    Wei Fei Diao
    Department of Pediatrics, Medical University of Vienna, Waehringer Guertel 18, A 1090, Vienna, Austria
    Hippocampus 17:563-76. 2007
    ..Results from this study, although increasing complexity of protein knowledge, may help to design further investigations at the protein level and may assist to interpret literature on protein expression and brain protein levels...
  71. doi Gel-based mass spectrometric analysis of a strongly hydrophobic GABAA-receptor subunit containing four transmembrane domains
    Sung Ung Kang
    Department of Pediatrics, Medical University of Vienna, Vienna, Austria
    Nat Protoc 4:1093-102. 2009
    ..The protocol takes approximately 10 d to complete and may represent a step forward in the complex analysis of other membrane or hydrophobic proteins...
  72. ncbi Increased RNA levels of the 25 kDa synaptosomal associated protein in brain samples of adult patients with Down Syndrome
    Susanne Greber-Platzer
    Department of Pediatrics, University of Vienna, Wahringer Gurtel 18 20, 1090 Vienna, Austria
    Neurosci Lett 336:77-80. 2003
    ..We suggest compensatory mechanisms for the upregulation at the transcriptional level. We propose that SNAP-25 as many other brain proteins are regulated by protein stability rather than at the mRNA level...
  73. ncbi Proteome profiling in the rat Harderian gland
    Jae Won Yang
    Department of Pediatrics, Medical University of Vienna, Vienna, Austria
    J Proteome Res 5:1751-62. 2006
    ....
  74. ncbi Hypothetical proteins with putative enzyme activity in human amnion, lymphocyte, bronchial epithelial and kidney cell lines
    Leila Afjehi-Sadat
    Department of Pediatrics, Division of Basic Science, University of Vienna, Wahringer Gurtel 18, A 1090 Vienna, Austria
    Biochim Biophys Acta 1700:65-74. 2004
    ..Furthermore, analytical data given are of major relevance as pIs, a prerequisite to find proteins in a map, cannot be predicted from nucleic acid sequences...
  75. ncbi Structural and functional analysis of hypothetical proteins in mouse hippocampus from two-dimensional gel electrophoresis
    Leila Afjehi-Sadat
    Department of Pediatrics, Medical University of Vienna, Waehringer Guertel 18 20, A 1090 Vienna, Austria
    J Proteome Res 6:711-23. 2007
    ..Three identified HPs were extracted from gels and renatured, and pyridoxal phosphate phosphatase, inorganic pyrophosphate phosphatase, and antioxidant activities were revealed, findings in agreement with functional predictions...
  76. ncbi Long-term effect of moderate and profound hypothermia on morphology, neurological, cognitive and behavioural functions in a rat model of perinatal asphyxia
    H Hoeger
    Institute for Animal Breeding, Medical University of Vienna, Vienna, Austria
    Amino Acids 31:385-96. 2006
    ..Perinatal asphyxia is a frequent cause of neurological handicap with no known therapy. However, hypothermic therapy has recently attracted attention owing to its neuroprotective property in brain of immature organisms...
  77. ncbi Using the quantitative competitive RT-PCR technique to analyze minute amounts of different mRNAs in small tissue samples
    Susanne Greber-Platzer
    Division of Pediatric Cardiology, Department of Pediatrics, University of Vienna, Austria
    Methods Mol Biol 193:29-58. 2002
  78. ncbi Aberrant expression of centractin and capping proteins, integral constituents of the dynactin complex, in fetal down syndrome brain
    Talin Gulesserian
    Department of Pediatrics, University of Vienna, Waehringer Guertel 18, Vienna, A 1090, Austria
    Biochem Biophys Res Commun 291:62-7. 2002
    ..Deranged centractins and F-actin capping proteins may represent or induce deficient axonal transport and may well contribute to deterioration of the cytoskeleton's mitotic functions in trisomy 21...
  79. ncbi Proteomic evaluation of intermediary metabolism enzyme proteins in fetal Down's syndrome cerebral cortex
    Michal Bajo
    University of Vienna, Department of Pediatrics, Vienna, Austria
    Proteomics 2:1539-46. 2002
    ..These results suggest derangement of intermediary metabolism during prenatal development of DS individuals...
  80. ncbi Mass spectrometrical identification of brain proteins including highly insoluble and transmembrane proteins
    Anna Bierczynska-Krzysik
    Department of Pediatrics, Medical University of Vienna, Waehringer Guertel 18, A 1090 Vienna, Austria
    Neurochem Int 49:245-55. 2006
    ..The fact that transmembrane, basic, acidic as well as hydrophobic proteins with a positive Gravy Index can be resolved warrants work on further improvement of this analytical tool...
  81. ncbi Proteome analysis of primary neurons and astrocytes from rat cerebellum
    Jae Won Yang
    Department of Pediatrics, Medical University of Vienna, Waehringer Guertel 18 20, A 1090 Vienna, Austria
    J Proteome Res 4:768-88. 2005
    ..The concomitant expressional patterns of several protein classes are given and potential neuronal and astrocytic marker candidates are presented...
  82. ncbi An integrated map of the murine hippocampal proteome based upon five mouse strains
    Daniela D Pollak
    Department of Pediatrics, Division of Pediatric Neuroscience, Medical University of Vienna, Austria
    Electrophoresis 27:2787-98. 2006
    ..Proteomic profiling of the hippocampus, a brain region with a pivotal role for neuronal information processing and storage may provide insight into the characteristics of proteins serving this highly sophisticated function...
  83. ncbi Proteomic analysis of rat cerebral cortex, hippocampus and striatum after exposure to morphine
    Anna Bierczynska-Krzysik
    Department of Chemistry, Neurobiochemistry Unit, Jagiellonian University, Krakow, Poland
    Int J Mol Med 18:775-84. 2006
    ..Moreover, the study reveals morphine-regulated proteins in different brain areas and indicates the pathways involved following morphine administration in the rat, the main species for pharmacological studies in the field...
  84. ncbi Proteomic analysis of the mouse brain following protein enrichment by preparative electrophoresis
    Elena Xixi
    Academy of Athens, Foundation for Biomedical Research, Division of Biotechnology, Athens, Greece
    Electrophoresis 27:1424-31. 2006
    ..Preparative electrophoresis is an efficient method for the enrichment of proteins of low molecular mass and may be useful in the investigation of disorders of the central nervous system...
  85. ncbi Proteomic analysis of the fetal brain
    Michael Fountoulakis
    Center for Medical Genomics, F Hoffmann La Roche, Basel, Switzerland
    Proteomics 2:1547-76. 2002
    ..It may be a useful tool in the investigation of protein changes in neurodegenerative diseases early in life...
  86. doi Gel-based mass spectrometric analysis of recombinant GABA(A) receptor subunits representing strongly hydrophobic transmembrane proteins
    Sung Ung Kang
    Department of Pediatrics, Division of Biochemistry and Molecular Biology, Center for Brain Research, Medical University of Vienna, Austria
    J Proteome Res 7:3498-506. 2008
    ..Proteins were cleaved by multienzyme proteolysis and subjected to nano-ESI-LC-MS/MS. Both GABA(A) receptor subunits were well-separated and unambiguously identified by sequence coverage of 99.1% (alpha1) and 92.9% (beta3)...
  87. ncbi Expression patterns of antioxidant proteins in brains of patients with sporadic Creutzfeldt-Jacob disease
    Kurt Krapfenbauer
    F Hoffmann La Roche Ltd, Pharmaceuticals Division, Basel, Switzerland
    Electrophoresis 23:2541-7. 2002
    ....
  88. ncbi Components of the protein quality control system are expressed in a strain-dependent manner in the mouse hippocampus
    Daniela D Pollak
    Department of Pediatrics, Division of Pediatric Neuroscience, Medical University of Vienna, Wahringer Gurtel 18, A 1090 Vienna, Austria
    Neurochem Int 49:500-7. 2006
    ..The findings are useful for designing future studies on these cascades and interpretation of results show that data on brain protein levels cannot be simply extrapolated among different mouse strains...
  89. ncbi Human brain nascent polypeptide-associated complex alpha subunit is decreased in patients with Alzheimer' s disease and Down syndrome
    Seong Hwan Kim
    Department of Pediatrics, University of Vienna, Austria
    J Investig Med 50:293-301. 2002
    ..Decreased alpha-NAC may result in the mistargeting, mistranslation, and proteolysis of proteins by affecting overall NAC function...
  90. ncbi Use of solution-IEF-fractionation leads to separation of 2673 mouse brain proteins including 255 hydrophobic structures
    Jae Kyung Myung
    Department of Pediatrics, Medical University of Vienna, Vienna, Austria
    J Proteome Res 5:1267-75. 2006
    ..This nonsophisticated approach to increase protein identification of a brain protein extract is a step forward in neurochemistry...
  91. ncbi Drebrin, a dendritic spine protein, is manifold decreased in brains of patients with Alzheimer's disease and Down syndrome
    Ki Shuk Shim
    Department of Pediatrics, University of Vienna, Waehringer Guertel 18, A 1090 Vienna, Austria
    Neurosci Lett 324:209-12. 2002
    ..Reduced drebrin could be responsible for or representing the loss of spine plasticity in DS and may be a useful indicator for the impaired arborization in neurodegenerative disorders...
  92. ncbi Human brain cytosolic histamine-N-methyltransferase is decreased in Down syndrome and increased in Pick's disease
    Seong Hwan Kim
    Department of Pediatrics, University of Vienna, Waehringer Guertel 18, A 1090 Vienna, Austria
    Neurosci Lett 321:169-72. 2002
    ..Decreased HMT in DS would be compatible with findings of decreased histamine synthesis, thus reflecting a compensation mechanism to antagonize reduced synthesis by decreased degradation...
  93. ncbi Mass spectrometrical verification of stomatin-like protein 2 (SLP-2) primary structure
    Julius Paul Pradeep John
    Department of Pediatrics, Medical University of Vienna, Vienna, Austria
    Proteins 64:543-51. 2006
    ..SLP-2 is a high abundance protein in several tissues and cells and may play an important biological role and therefore characterization of its primary structure is of importance...
  94. ncbi Changes of hippocampal protein levels during postnatal brain development in the rat
    Rachel Weitzdörfer
    Medical University of Vienna, Department of Neonatology, Vienna, Austria
    J Proteome Res 5:3205-12. 2006
    ..g., antioxidant, metabolic, cytoskeleton, proteasomal, and chaperone pathways were developmentally regulated, which is relevant for design and interpretation of protein chemical studies in the mammalian brain...
  95. ncbi Reduced levels of DEAD-box proteins DBP-RB and p72 in fetal Down syndrome brains
    Susanne G Kircher
    Institute of Medical Chemistry, University of Vienna, Austria
    Neurochem Res 27:1141-6. 2002
    ....
  96. ncbi Metalloproteinase ADAMTS-1 but not ADAMTS-5 is manifold overexpressed in neurodegenerative disorders as Down syndrome, Alzheimer's and Pick's disease
    Rosa Ferrando Miguel
    CChem, FRSC UK, Medical University of Vienna, Department of Pediatrics, Wahringer Gurtel 18, A 1090 Vienna, Austria
    Brain Res Mol Brain Res 133:1-5. 2005
    ..The manifold overexpression of ADAMTS-1 may be used as marker protein for neurodegeneration...
  97. ncbi The rat brain hippocampus proteome
    Michael Fountoulakis
    F Hoffmann La Roche Ltd, Center for Medical Genomics, Basel, Switzerland
    J Chromatogr B Analyt Technol Biomed Life Sci 819:115-29. 2005
    ..It also includes 39 neuron specific gene products. The database may be useful in animal model studies of neurological disorders...
  98. ncbi Molecular changes in fetal Down syndrome brain
    Ephrem Engidawork
    Department of Pediatrics, University of Vienna, Wahringer Gurtel 18 20, A 1090 Vienna, Austria
    J Neurochem 84:895-904. 2003
    ..We propose that these biochemical alterations represent and/or precede morphological changes. This review attempts to dissect these molecular changes and to explain how they may lead to mental retardation...
  99. ncbi Protein profile of the HeLa cell line
    Michael Fountoulakis
    F Hoffmann La Roche Ltd, Center for Medical Genomics, Building 93 444, Basel CH 4070, Switzerland
    J Chromatogr A 1038:247-65. 2004
    ....
  100. ncbi Proteome analysis of mouse primary astrocytes
    Jae Won Yang
    Department of Pediatrics, Medical University of Vienna, Waehringer Guertel 18 20, A 1090 Vienna, Austria
    Neurochem Int 47:159-72. 2005
    ..A reliable analytical tool independent of antibody availability and specificity along with tentative astrocytic marker proteins is described...
  101. ncbi Fetal Down syndrome brains exhibit aberrant levels of neurotransmitters critical for normal brain development
    Nigel Whittle
    Department of Pharmacology and Toxicology, Institute of Pharmacy and Center for Molecular Biosciences, University of Innsbruck, Innsbruck, Austria
    Pediatrics 120:e1465-71. 2007
    ..However, mechanisms that underlie the observed dysfunctional brain development in Down syndrome are not clear...