Renate Kunert

Summary

Affiliation: University of Natural Resources and Applied Life Sciences
Country: Austria

Publications

  1. ncbi request reprint Validated method for quantification of genetically modified organisms in samples of maize flour
    Renate Kunert
    Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
    J Agric Food Chem 54:678-81. 2006
  2. doi request reprint Strategies for efficient transfection of CHO-cells with plasmid DNA
    Renate Kunert
    Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria
    Methods Mol Biol 801:213-26. 2012
  3. doi request reprint CHO-recombinant human growth hormone as a protease sensitive reporter protein
    Renate Kunert
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, 1190, Vienna, Austria
    Appl Microbiol Biotechnol 84:693-9. 2009
  4. ncbi request reprint Process parameter shifting: Part II. Biphasic cultivation-A tool for enhancing the volumetric productivity of batch processes using Epo-Fc expressing CHO cells
    Evelyn Trummer
    Department of Biotechnology, Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences Vienna, Muthgasse 18, 1190 Vienna, Austria
    Biotechnol Bioeng 94:1045-52. 2006
  5. pmc Improved virus neutralization by plant-produced anti-HIV antibodies with a homogeneous beta1,4-galactosylated N-glycan profile
    Richard Strasser
    Department of Applied Genetics and Cell Biology, University of Natural Resources and Applied Life Sciences, 1190 Vienna, Austria
    J Biol Chem 284:20479-85. 2009
  6. ncbi request reprint Protein-free transfection of CHO host cells with an IgG-fusion protein: selection and characterization of stable high producers and comparison to conventionally transfected clones
    Christine Lattenmayer
    Austrian Center of Biopharmaceutical Technology, Muthgasse 18, 1190 Vienna, Austria
    Biotechnol Bioeng 96:1118-26. 2007
  7. doi request reprint Transcriptional profiling of phenotypically different Epo-Fc expressing CHO clones by cross-species microarray analysis
    Evelyn Trummer
    Department of Biotechnology, Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences Vienna, Vienna, Austria
    Biotechnol J 3:924-37. 2008
  8. doi request reprint Expression, purification, and in vivo administration of a promising anti-idiotypic HIV-1 vaccine
    Johannes Simon Gach
    Institute of Applied Microbiology, Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
    Mol Biotechnol 39:119-25. 2008
  9. pmc Expression of antibody fragments with a controlled N-glycosylation pattern and induction of endoplasmic reticulum-derived vesicles in seeds of Arabidopsis
    Andreas Loos
    Department of Applied Genetics and Cell Biology, University of Natural Resources and Applied Life Sciences, A 1190 Vienna, Austria
    Plant Physiol 155:2036-48. 2011
  10. ncbi request reprint Applicability of different fluorescent dyes for isoform quantification on linear IPG gels
    Kornelia Schriebl
    Austrian Center of Biopharmaceutical Technology, Vienna, Austria
    Electrophoresis 28:2100-7. 2007

Detail Information

Publications68

  1. ncbi request reprint Validated method for quantification of genetically modified organisms in samples of maize flour
    Renate Kunert
    Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
    J Agric Food Chem 54:678-81. 2006
    ..The results were evaluated with ValiData for homology of variance, linearity, accuracy of the standard curve, and standard deviation...
  2. doi request reprint Strategies for efficient transfection of CHO-cells with plasmid DNA
    Renate Kunert
    Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria
    Methods Mol Biol 801:213-26. 2012
    ..Here, we describe protocols for optimizing expression in transient expression experiments and isolating stable CHO cell lines using two types of self-made reagents, namely, lipoplexes and polyplexes...
  3. doi request reprint CHO-recombinant human growth hormone as a protease sensitive reporter protein
    Renate Kunert
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, 1190, Vienna, Austria
    Appl Microbiol Biotechnol 84:693-9. 2009
    ..Analysis of the digested molecule was then performed by convergent methods like SDS-PAGE, HPLC and mass spectroscopy, and the results indicate hGH to be an ideal candidate for media and component screening in mammalian cell culture...
  4. ncbi request reprint Process parameter shifting: Part II. Biphasic cultivation-A tool for enhancing the volumetric productivity of batch processes using Epo-Fc expressing CHO cells
    Evelyn Trummer
    Department of Biotechnology, Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences Vienna, Muthgasse 18, 1190 Vienna, Austria
    Biotechnol Bioeng 94:1045-52. 2006
    ....
  5. pmc Improved virus neutralization by plant-produced anti-HIV antibodies with a homogeneous beta1,4-galactosylated N-glycan profile
    Richard Strasser
    Department of Applied Genetics and Cell Biology, University of Natural Resources and Applied Life Sciences, 1190 Vienna, Austria
    J Biol Chem 284:20479-85. 2009
    ....
  6. ncbi request reprint Protein-free transfection of CHO host cells with an IgG-fusion protein: selection and characterization of stable high producers and comparison to conventionally transfected clones
    Christine Lattenmayer
    Austrian Center of Biopharmaceutical Technology, Muthgasse 18, 1190 Vienna, Austria
    Biotechnol Bioeng 96:1118-26. 2007
    ....
  7. doi request reprint Transcriptional profiling of phenotypically different Epo-Fc expressing CHO clones by cross-species microarray analysis
    Evelyn Trummer
    Department of Biotechnology, Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences Vienna, Vienna, Austria
    Biotechnol J 3:924-37. 2008
    ..These promising results will further increase our efforts to develop CHO specific microarrays that deliver information about the suitability of a clone candidate for industrial production...
  8. doi request reprint Expression, purification, and in vivo administration of a promising anti-idiotypic HIV-1 vaccine
    Johannes Simon Gach
    Institute of Applied Microbiology, Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
    Mol Biotechnol 39:119-25. 2008
    ..The crude sera were screened for specific antibodies against the epitope of mAb 2F5 ELDKWA as well as for reactivity against HIV-1 gp41...
  9. pmc Expression of antibody fragments with a controlled N-glycosylation pattern and induction of endoplasmic reticulum-derived vesicles in seeds of Arabidopsis
    Andreas Loos
    Department of Applied Genetics and Cell Biology, University of Natural Resources and Applied Life Sciences, A 1190 Vienna, Austria
    Plant Physiol 155:2036-48. 2011
    ....
  10. ncbi request reprint Applicability of different fluorescent dyes for isoform quantification on linear IPG gels
    Kornelia Schriebl
    Austrian Center of Biopharmaceutical Technology, Vienna, Austria
    Electrophoresis 28:2100-7. 2007
    ..g., clone screening, process optimization, and purification monitoring...
  11. ncbi request reprint Biochemical characterization of rhEpo-Fc fusion protein expressed in CHO cells
    Kornelia Schriebl
    Austrian Center of Biopharmaceutical Technology, Muthgasse 18, A 1190 Vienna, Austria
    Protein Expr Purif 49:265-75. 2006
    ..The direct comparison with other rhEpo-Fc fusion proteins failed, because no appropriate data were found in the literature...
  12. doi request reprint IgM characterization directly performed in crude culture supernatants by a new simple electrophoretic method
    Karola Vorauer-Uhl
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
    J Immunol Methods 359:21-7. 2010
    ....
  13. pmc In planta protein sialylation through overexpression of the respective mammalian pathway
    Alexandra Castilho
    Department of Applied Genetics and Cell Biology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, 1190 Vienna, Austria
    J Biol Chem 285:15923-30. 2010
    ..Besides the biotechnological impact of the achievement, this work may serve as a general model for the manipulation of complex traits into plants...
  14. doi request reprint Growth, productivity and protein glycosylation in a CHO EpoFc producer cell line adapted to glutamine-free growth
    Michael Taschwer
    Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria
    J Biotechnol 157:295-303. 2012
    ..Except for this, the glycosylation, with respect to site occupancy, degree of sialylation and glycoform structure, was highly comparable, both for the Epo and the Fc part of the protein...
  15. doi request reprint Topological transformation of liposomes by a membrane-affecting domain of recombinant human erythropoietin
    Stefanie Strobach
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria
    J Liposome Res 20:24-30. 2010
    ..Since this helix B of rh-Epo has recently been supposed to be involved in the activation of neuroprotective pathways, we believe that the membrane-transforming capacity of rh-Epo participates in the proliferative activity of rh-Epo...
  16. ncbi request reprint High level expression of a promising anti-idiotypic antibody fragment vaccine against HIV-1 in Pichia pastoris
    Johannes S Gach
    Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
    J Biotechnol 128:735-46. 2007
    ..Biochemical analysis confirmed that the N-glycosylation does not contribute to the binding properties of 3H6...
  17. ncbi request reprint A novel strategy for quantitative isoform detection directly performed from culture supernatant
    Kornelia Schriebl
    Austrian Center of Biopharmaceutical Technology, Muthgasse 18, A 1190 Vienna, Austria
    J Pharm Biomed Anal 42:322-7. 2006
    ..A fundamental benefit of this technique is the ability to perform quantitative isoform pattern analysis directly from serum-free culture supernatant...
  18. ncbi request reprint Process parameter shifting: Part I. Effect of DOT, pH, and temperature on the performance of Epo-Fc expressing CHO cells cultivated in controlled batch bioreactors
    Evelyn Trummer
    Department of Biotechnology, Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences Vienna, Muthgasse 18, 1190 Vienna, Austria
    Biotechnol Bioeng 94:1033-44. 2006
    ..The well directed alteration of the process environment has emerged as a tool adequate for further process optimization applying a biphasic cultivation strategy...
  19. ncbi request reprint Characterisation of recombinant CHO cell lines by investigation of protein productivities and genetic parameters
    Christine Lattenmayer
    Austrian Center of Biopharmaceutical Technology, Muthgasse 18, 1190 Vienna, Austria
    J Biotechnol 128:716-25. 2007
    ..Nevertheless, a wider range of cell lines has to be investigated in order to implement genetic analyses into a screening process...
  20. doi request reprint A plant-derived human monoclonal antibody induces an anti-carbohydrate immune response in rabbits
    Chunsheng Jin
    Department of Chemistry, University of Natural Resources and Applied Life Sciences, Muthgasse 18, 1190 Vienna, Austria
    Glycobiology 18:235-41. 2008
    ..This emphasizes the need for the use of glyco-engineered plants lacking any potentially antigenic N-glycan structures for the production of plant-derived recombinant proteins intended for parenteral human application...
  21. doi request reprint A screening method to assess biological effects of microRNA overexpression in Chinese hamster ovary cells
    Vaibhav Jadhav
    Department of Biotechnology, University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria
    Biotechnol Bioeng 109:1376-85. 2012
    ..Thus our system represents a general platform to functionally screen candidates for rational cell factory design...
  22. ncbi request reprint Structural analysis and in vivo administration of an anti-idiotypic antibody against mAb 2F5
    Johannes S Gach
    Institute of Applied Microbiology, Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
    Mol Immunol 45:1027-34. 2008
    ..Moreover, we report about an immunisation study with Ab2/3H6 Fab variants, which elicited a specific 2F5-like humoral immune response in BALB/c mice...
  23. ncbi request reprint Characterization of molecular features, antigen-binding, and in vitro properties of IgG and IgM variants of 4E10, an anti-HIV type 1 neutralizing monoclonal antibody
    Renate Kunert
    Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, A 1190 Vienna, Austria
    AIDS Res Hum Retroviruses 20:755-62. 2004
    ..The antiviral activity could be greatly enhanced by change of IgG(3) to IgG(1). In contrast, the IgM isotype almost completely lost its neutralizing potential...
  24. doi request reprint The absence of effect of gene copy number and mRNA level on the amount of mAb secretion from mammalian cells
    Hannes Reisinger
    Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences Vienna, Muthgasse 18, 1190, Vienna, Austria
    Appl Microbiol Biotechnol 81:701-10. 2008
    ..In our example, translational and post-translational limitations are responsible for decreased antibody secretion...
  25. doi request reprint A study on the temperature dependency and time course of the cold capture antibody secretion assay
    Johannes Pichler
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Institute of Applied Microbiology, Vienna, Austria
    J Biotechnol 141:80-3. 2009
    ..Our results indicate that the secreted product is trapped by the staining antibody on the cell surface at all temperatures. Subsequently these aggregates are endocytosed by the cells, a process which is slowed down at low temperatures...
  26. ncbi request reprint Partial humanization and characterization of an anti-idiotypic antibody against monoclonal antibody 2F5, a potential HIV vaccine?
    Johannes S Gach
    Institute of Applied Microbiology, Department of Biotechnology, University of Natural Resources and Applied Life Sciences, A 1190 Vienna, Austria
    AIDS Res Hum Retroviruses 23:1405-15. 2007
    ..Moreover, the inhibition potency of chimeric Ab2/3H6 analyzed by in vitro studies could be shown to be the same as that detected for the hybridoma-derived murine Ab2/3H6...
  27. pmc Identification of transgene integration loci of different highly expressing recombinant CHO cell lines by FISH
    Christine Lattenmayer
    Austrian Center of Biopharmaceutical Technology, Muthgasse 18, 1190, Vienna, Austria
    Cytotechnology 51:171-82. 2006
    ..Furthermore, except from two cell lines, the exogene was integrated at a single site. The dhfr selection marker was co-localised to the target gene...
  28. pmc Proline is not uniquely capable of providing the pivot point for domain swapping in 2G12, a broadly neutralizing antibody against HIV-1
    Johannes S Gach
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, 1190 Vienna, Austria
    J Biol Chem 285:1122-7. 2010
    ..Here, we further define the amino acids responsible for this extraordinary domain-swapping event in 2G12...
  29. ncbi request reprint Recombinant IgA production: single step affinity purification using camelid ligands and product characterization
    David Reinhart
    Department of Biotechnology, University of Natural Resources and Life Sciences, Muthgasse 11, 1190 Vienna, Austria
    J Immunol Methods 378:95-101. 2012
    ..Results were compared with a multistep purification process consisting of an affinity step based on immobilized jacalin followed by anion exchange and hydrophobic interaction chromatography...
  30. ncbi request reprint Screening for improved cell performance: selection of subclones with altered production kinetics or improved stability by cell sorting
    Ernst Böhm
    Institute of Applied Microbiology, University of Agricultural Sciences, Muthgasse 18, 1190 Vienna, Austria
    Biotechnol Bioeng 88:699-706. 2004
    ..The two desired cellular properties screened for were a non-growth associated production kinetic and improved stability in the absence of selective pressure...
  31. ncbi request reprint Effect of increased expression of protein disulfide isomerase and heavy chain binding protein on antibody secretion in a recombinant CHO cell line
    Nicole Borth
    Institute for Applied Microbiology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, 1190 Vienna, Austria
    Biotechnol Prog 21:106-11. 2005
    ..Overexpression of BIP, both alone and in combination with PDI, reduced the specific secretion rate, whereas PDI, when overexpressed alone, caused an increase of product secretion rate...
  32. doi request reprint Analysis of immunoglobulin glycosylation by LC-ESI-MS of glycopeptides and oligosaccharides
    Johannes Stadlmann
    Department of Chemistry, University of Natural Resources and Applied Life Sciences BOKU, Vienna, Austria
    Proteomics 8:2858-71. 2008
    ..Regarding fast and yet detailed structural assignment, LC-MS on graphitic carbon supersedes all other current methods...
  33. ncbi request reprint Improvement of the energy metabolism of recombinant CHO cells by cell sorting for reduced mitochondrial membrane potential
    Georg Hinterkörner
    Institute for Applied Microbiology, Department of Biotechnology, Universität für Bodenkultur, Muthgasse 18, Vienna, Austria
    J Biotechnol 129:651-7. 2007
    ..The potential of using flow cytometric cell sorting methods based on physiological activity for cell line optimisation is discussed...
  34. doi request reprint A close look at human IgG sialylation and subclass distribution after lectin fractionation
    Johannes Stadlmann
    Department of Chemistry, University of Natural Resources and Applied Life Sciences BOKU, Vienna, Austria
    Proteomics 9:4143-53. 2009
    ..This observation may be relevant when studying the effect of human Fc in murine models of inflammation because mouse IgG subclasses differ substantially in their interaction with receptors...
  35. ncbi request reprint Establishment of a strategy for the rapid generation of a monoclonal antibody against the human protein SNEV (hNMP200) by flow-cytometric cell sorting
    Ernst Böhm
    Institute of Applied Microbiology, Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Wien Vienna, Austria
    J Immunol Methods 307:13-23. 2005
    ....
  36. pmc Evaluation of the potency of the anti-idiotypic antibody Ab2/3H6 mimicking gp41 as an HIV-1 vaccine in a rabbit prime/boost study
    Alexander Mader
    Department of Biotechnology, VIBT BOKU University of Natural Resources and Life Sciences Vienna, Vienna, Austria
    PLoS ONE 7:e39063. 2012
    ..Despite specificity for the linear epitope and the truncated HIV-1 envelope protein these antibodies were not able to exhibit virus neutralization activities. These results suggest that Ab2/3H6 alone might not be suitable as a vaccine...
  37. ncbi request reprint HIV-1 mutants escaping neutralization by the human antibodies 2F5, 2G12, and 4E10: in vitro experiments versus clinical studies
    Sabine Nakowitsch
    Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, Vienna, Austria
    AIDS 19:1957-66. 2005
    ..Although they have been shown to prevent de novo infection in vivo, their potential for treatment of chronic infection is less clear. One major obstacle may be the emergence of resistant viruses during mAb treatment...
  38. doi request reprint Production of monoclonal antibodies with a controlled N-glycosylation pattern in seeds of Arabidopsis thaliana
    Andreas Loos
    Department of Applied Genetics and Cell Biology, University of Natural Resources and Applied Life Sciences, Vienna, Austria
    Plant Biotechnol J 9:179-92. 2011
    ....
  39. ncbi request reprint Reassessment of autoreactivity of the broadly neutralizing HIV antibodies 4E10 and 2F5 and retrospective analysis of clinical safety data
    Brigitta Vcelar
    Polymun Scientific, Vienna bImmunology Outpatient Clinic, Vienna, Austria
    AIDS 21:2161-70. 2007
    ..The broadly neutralizing recombinant human HIV-1 antibodies 4E10, 2F5 and Igh1b12 are reported to have autoreactive potential, which is significant for HIV-1 vaccine development and passive immunotherapy using these antibodies...
  40. doi request reprint Exploration of BAC versus plasmid expression vectors in recombinant CHO cells
    Alexander Mader
    Department of Biotechnology, VIBT, BOKU University of Natural Resources and Life Sciences Vienna, Muthgasse 11, 1190 Vienna, Austria
    Appl Microbiol Biotechnol 97:4049-54. 2013
    ..This study indicated comparable increase of transcription level for both scFc proteins when using the BAC system, but translation, maturation, and secretion of individual proteins seem to be protein specific...
  41. pmc Serum-free transfection of CHO-cells with tailor-made unilamellar vesicles
    Hannes Reisinger
    Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences Vienna, Muthgasse 18, 1190, Vienna, Austria
    Cytotechnology 54:157-68. 2007
    ....
  42. ncbi request reprint Engineering of Pichia pastoris for improved production of antibody fragments
    Brigitte Gasser
    Institute of Applied Microbiology, BOKU University of Natural Resources and Applied Life Sciences Vienna, Muthgasse 18, A 1190 Vienna, Austria
    Biotechnol Bioeng 94:353-61. 2006
    ..3-fold, PDI enabled an increase of the Fab level by 1.9-fold. Hence, the formation of interchain disulfide bonds can be seen as a major rate limiting factor to Fab assembly and subsequent secretion...
  43. pmc Next-generation sequencing of the Chinese hamster ovary microRNA transcriptome: Identification, annotation and profiling of microRNAs as targets for cellular engineering
    Matthias Hackl
    Department of Biotechnology, University of Natural Resources and Life Sciences Vienna, Muthgasse 19, A 1190 Vienna, Austria
    J Biotechnol 153:62-75. 2011
    ....
  44. ncbi request reprint Antiviral activity of the neutralizing antibodies 2F5 and 2G12 in asymptomatic HIV-1-infected humans: a phase I evaluation
    Gabriela Stiegler
    Institute of Applied Microbiology, University of Agricultural Sciences, Muthgasse, Vienna, Austria
    AIDS 16:2019-25. 2002
    ..However, the antiviral effects of antibody treatment have not been fully analyzed in this first clinical trial...
  45. pmc Recent advances in recombinant protein production: BAC-based expression vectors, the bigger the better
    Renate Kunert
    Department of Biotechnology, Vienna Institute of BioTechnology, University of Natural Resources and Life Sciences, Vienna, Vienna, Austria
    Bioengineered 4:258-61. 2013
    ..These properties make BAC-based expression vectors a very attractive tool applied to the recombinant protein production field. ..
  46. pmc Serum-free transfection of CHO cells with chemically defined transfection systems and investigation of their potential for transient and stable transfection
    Hannes Reisinger
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences Vienna, Muthgasse 18, Vienna, 1190, Austria
    Cytotechnology 60:115-23. 2009
    ..These experiments serve to demonstrate the potential of stable cell lines even in case where the transient systems did not show satisfying results...
  47. doi request reprint Comparison of hybridization methods and real-time PCR: their value in animal cell line characterization
    Helga Böhm-Hofstätter
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Muthgasse 11, 1190, Vienna, Austria
    Appl Microbiol Biotechnol 87:419-25. 2010
    ..A comparative review of the literature as well as an overview of findings from our own group will be given...
  48. pmc Characterization of human class-switched polymeric (immunoglobulin M [IgM] and IgA) anti-human immunodeficiency virus type 1 antibodies 2F5 and 2G12
    Susanne Wolbank
    Institute of Applied Microbiology, University of Agriculture, A 1190 Vienna, Austria
    J Virol 77:4095-103. 2003
    ....
  49. pmc One-step triplex high-resolution melting analysis for rapid identification and simultaneous subtyping of frequently isolated Salmonella serovars
    Josef Zeinzinger
    Austrian Agency for Health and Food Safety, Institute of Medical Microbiology and Hygiene, Vienna, Austria
    Appl Environ Microbiol 78:3352-60. 2012
    ..These 39 serotypes represent more than 94% of all human and more than 85% of all nonhuman Salmonella isolates (including isolates from veterinary, food, and environmental samples) obtained in the years 2008 and 2009 in Austria...
  50. pmc The long third complementarity-determining region of the heavy chain is important in the activity of the broadly neutralizing anti-human immunodeficiency virus type 1 antibody 2F5
    Michael B Zwick
    Department of Immunology, The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Virol 78:3155-61. 2004
    ..These observations present a challenge to vaccine strategies based on peptide mimics of the linear epitope...
  51. pmc Comprehensive cross-clade neutralization analysis of a panel of anti-human immunodeficiency virus type 1 monoclonal antibodies
    James M Binley
    IMM2, Department of Immunology, The Scripps Research Institute, 10550 N Torrey Pines Rd, La Jolla, CA 92037, USA
    J Virol 78:13232-52. 2004
    ..As well as the significance for vaccine design, our data have implications for passive-immunization studies in countries where clade C viruses are common, given that only MAbs b12 and 4E10 were effective against viruses from this clade...
  52. pmc Binding of the 2F5 monoclonal antibody to native and fusion-intermediate forms of human immunodeficiency virus type 1 gp41: implications for fusion-inducing conformational changes
    Eve de Rosny
    Center for Biologics Evaluation and Research, U S Food and Drug Administration, Bethesda, Maryland 20892 4555, USA
    J Virol 78:2627-31. 2004
    ..We also demonstrate conformational changes in the C heptad of gp41...
  53. ncbi request reprint Antibody domain exchange is an immunological solution to carbohydrate cluster recognition
    Daniel A Calarese
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 300:2065-71. 2003
    ..The unique interdigitation of Fab domains within an antibody uncovers a previously unappreciated mechanism for high-affinity recognition of carbohydrate or other repeating epitopes on cell or microbial surfaces...
  54. pmc Anti-human immunodeficiency virus type 1 (HIV-1) antibodies 2F5 and 4E10 require surprisingly few crucial residues in the membrane-proximal external region of glycoprotein gp41 to neutralize HIV-1
    Michael B Zwick
    Department of Immunology IMM 2, The Scripps Research Institute, 10550 North Torrey Pines Rd, La Jolla, CA 92037, USA
    J Virol 79:1252-61. 2005
    ..Neutralization experiments showing synergy between and T20 and 4E10 against HIV-1 are also presented. The data presented may aid in the design of antigens that better present the MPER of gp41 to the immune system...
  55. ncbi request reprint Broadly neutralizing anti-HIV antibody 4E10 recognizes a helical conformation of a highly conserved fusion-associated motif in gp41
    Rosa M F Cardoso
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Immunity 22:163-73. 2005
    ..These structural insights should assist in the design of immunogens to elicit 4E10-like neutralizing responses...
  56. ncbi request reprint Recombinant antibody 2G12 produced in maize endosperm efficiently neutralizes HIV-1 and contains predominantly single-GlcNAc N-glycans
    Thomas Rademacher
    Institute for Molecular Biotechnology, Biology VII, RWTH Aachen, Worringerweg 1, 52074 Aachen, Germany
    Plant Biotechnol J 6:189-201. 2008
    ....
  57. ncbi request reprint Cardiolipin polyspecific autoreactivity in two broadly neutralizing HIV-1 antibodies
    Barton F Haynes
    Duke University School of Medicine, Durham, NC 27710, USA
    Science 308:1906-8. 2005
    ..These results may have important implications for generating effective neutralizing antibody responses by using HIV-1 vaccines...
  58. ncbi request reprint Membrane association and epitope recognition by HIV-1 neutralizing anti-gp41 2F5 and 4E10 antibodies
    Silvia Sanchez-Martinez
    Biophysics Unit CSIC UPV EHU and Biochemistry Department, University of Basque Country, 48080 Bilbao, Spain
    AIDS Res Hum Retroviruses 22:998-1006. 2006
    ..We discuss the relevance of these observations for the design of immunogens aimed at eliciting 2F5- and 4E10- like humoral responses...
  59. doi request reprint Crystal structure of the complex between the F(ab)' fragment of the cross-neutralizing anti-HIV-1 antibody 2F5 and the F(ab) fragment of its anti-idiotypic antibody 3H6
    Steve Bryson
    Department of Biochemistry, University of Toronto, 1 King s College Circle, Toronto, Ontario, Canada M5S 1A8
    J Mol Biol 382:910-9. 2008
    ..This defines Ab2/3H6 as an anti-idiotypic antibody of the Ab2gamma class, i.e., an antigen-inhibitable idiotype that does not carry the internal image of the linear primary gp41 (662)ELDKWAS(668) epitope...
  60. ncbi request reprint Production of a monoclonal antibody in plants with a humanized N-glycosylation pattern
    Matthias Schähs
    Institute of Applied Genetics and Cell Biology, BOKU Wien, Austria
    Plant Biotechnol J 5:657-63. 2007
    ..Our results demonstrate the feasibility of a knockout strategy for N-glycan engineering of plants towards mammalian-like structures, thus providing a significant improvement in the use of plants as an expression platform...
  61. ncbi request reprint Functional analysis of the broadly neutralizing human anti-HIV-1 antibody 2F5 produced in transgenic BY-2 suspension cultures
    Markus Sack
    Institute of Molecular Biotechnology, RTWH Aachen University, Worringerweg 1, 52074 Aachen, Germany
    FASEB J 21:1655-64. 2007
    ..This highlights important issues raised by the use of plant systems to produce human biologics...
  62. ncbi request reprint Specific phospholipid recognition by human immunodeficiency virus type-1 neutralizing anti-gp41 2F5 antibody
    Silvia Sanchez-Martinez
    Biofisika Unitatea CSIC UPV EHU and Biokimika Saila, Euskal Herriko Unibertsitatea, Posta Kutxa 644, 48080 Bilbao, Spain
    FEBS Lett 580:2395-99. 2006
    ..These findings might be of relevance for understanding the molecular basis of HIV-1 immune evasion...
  63. ncbi request reprint Anti-neuroblastoma effect of ch14.18 antibody produced in CHO cells is mediated by NK-cells in mice
    Yan Zeng
    Charite Children s Hospital, University Medicine, 13353 Berlin, Germany
    Mol Immunol 42:1311-9. 2005
    ..In vivo depletion of NK-cells completely abrogated this effect, suggesting that the mechanism involved in the ch14.18/CHO induced anti-neuroblastoma effect is mediated by NK-dependent ADCC...
  64. pmc The broadly neutralizing anti-human immunodeficiency virus type 1 4E10 monoclonal antibody is better adapted to membrane-bound epitope recognition and blocking than 2F5
    Nerea Huarte
    Unidad de Biofisica CSIC UPV EHU, Universidad del Pais Vasco, Aptdo 644, 48080 Bilbao, Spain
    J Virol 82:8986-96. 2008
    ..We conclude that the capacity to interfere with the membrane activity of conserved MPER sequences is best correlated with the broad neutralization of the 4E10 MAb...
  65. ncbi request reprint Electron microscopic and immunochemical analysis of the broadly neutralizing HIV-1-specific, anti-carbohydrate antibody, 2G12
    K H Roux
    Department of Biological Science, 2nd Institute of Molecular Biophysics, Florida State University, Tallahassee, FL 32306 4307, USA
    Mol Immunol 41:1001-11. 2004
    ..Efforts to identify IgG molecules with a similar intertwined Fab dimer structure in a large IgG pool were unsuccessful...
  66. ncbi request reprint Recognition and blocking of HIV-1 gp41 pre-transmembrane sequence by monoclonal 4E10 antibody in a Raft-like membrane environment
    Maier Lorizate
    Biophysics Unit Consejo Superior de Investigaciones Científicas UPV EHU and Biochemistry Department, University of the Basque Country, P O Box 644, 48080 Bilbao, Spain
    J Biol Chem 281:39598-606. 2006
    ..This work comprises the first experimental report on a lipid-modulated antibody capacity to bind a membrane-bound epitope sequence and arrest its restructuring activity...
  67. pmc A peptide inhibitor of HIV-1 neutralizing antibody 2G12 is not a structural mimic of the natural carbohydrate epitope on gp120
    Alfredo Menendez
    Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, V5A 1S6, Canada
    FASEB J 22:1380-92. 2008
    ..Moreover, 2G12.1 peptide is not an immunogenic mimic of the 2G12 epitope, since antisera produced against it did not bind gp120...
  68. pmc Inhibition of human immunodeficiency virus type 1 entry in cells expressing gp41-derived peptides
    Marc Egelhofer
    Institute for Biomedical Research Georg Speyer Haus, Frankfurt a M Medizinische Hochschule Hannover, Hannover, Germany
    J Virol 78:568-75. 2004
    ..Preclinical toxicity and efficacy studies of this antiviral vector have been completed, and clinical trials are in preparation...