Research Topics
Genomes and Genes | Daniel KolarichSummaryAffiliation: University of Natural Resources and Applied Life Sciences Country: Austria Publications
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Detail Information
Publications
Molecular characterization and allergenic activity of Lyc e 2 (beta-fructofuranosidase), a glycosylated allergen of tomatoSandra Westphal
Department of Allergology, Paul Ehrlich Institut, Langen, Germany
Eur J Biochem 270:1327-37. 2003....
The N-glycans of yellow jacket venom hyaluronidases and the protein sequence of its major isoform in Vespula vulgarisDaniel Kolarich
Department of Chemistry, University of Natural Resources and Applied Life Sciences (BOKU, Vienna, Austria
FEBS J 272:5182-90. 2005..Close homologues of this new, putative hyaluronidase b (Ves v 2b) were also the major isoform in the other wasp venoms...
Comprehensive glyco-proteomic analysis of human alpha1-antitrypsin and its charge isoformsDaniel Kolarich
Department of Chemistry, Biochemistry Division, University of Natural Resources and Applied Life Sciences BOKU, Vienna, Austria
Proteomics 6:3369-80. 2006..Fucosylation of diantennary structures was marginal and of the core alpha1,6 type...
A proteomic study of the major allergens from yellow jacket venomsDaniel Kolarich
Biochemistry Division, Department of Chemistry, University of Natural Resources and Applied Life Sciences BOKU, Vienna, Austria
Proteomics 7:1615-23. 2007..vulgaris, germanica, maculifrons, pensylvanica, flavopilosa and squamosa revealed the latter as a taxonomic outlier and led to the discovery of several not previously reported amino acid differences...
Identification of four IgE-reactive proteins in raspberry (Rubus ideaeus L.)Gorji Marzban
Plant Biotechnology Unit, Department of Biotechnology, BOKU, Vienna, Austria
Mol Nutr Food Res 52:1497-506. 2008..Finally, MS/MS analysis revealed an IgE-reactive raspberry cyclophilin, homologous to Bet v 7. Results obtained suggest that the consumption of raspberries might be responsible for adverse reactions in sensitised individuals...
Biochemical, molecular characterization, and glycoproteomic analyses of alpha(1)-proteinase inhibitor products used for replacement therapyDaniel Kolarich
University of Natural Resources and Applied Life Sciences, Vienna, Austria
Transfusion 46:1959-77. 2006..Tri- and tetraantennary structures are responsible for the partitioning into IEF isoforms, with the migration shift of Aralast not being due to any difference in the N-glycosylation, but to the partial loss of the C-terminal lysine...
Molecular cloning and characterization of a plant alpha1,3/4-fucosidase based on sequence tags from almond fucosidase IReinhard Zeleny
Department of Chemistry, University of Natural Resources and Applied Biosciences Vienna, Muthgasse, A 1190 Vienna, Austria
Phytochemistry 67:641-8. 2006..The alpha1,3/4-fucosidase from Arabidopsis exhibited striking sequence similarity with an enzyme of similar substrate specificity from Streptomyces sp. (Q9Z4I9) and with putative proteins from rice...
Trichomonas vaginalis: metronidazole and other nitroimidazole drugs are reduced by the flavin enzyme thioredoxin reductase and disrupt the cellular redox system. Implications for nitroimidazole toxicity and resistanceDavid Leitsch
Department of Specific Prophylaxis and Tropical Medicine at the Center for Physiology, Pathophysiology and Immunology, Medical University of Vienna, Vienna, Austria
Mol Microbiol 72:518-36. 2009..Thus, taken together, our data suggest a flavin-based mechanism of metronidazole activation and thereby challenge the current model of hydrogenosomal activation of nitroimidazole drugs...
Glycoproteomic characterization of butyrylcholinesterase from human plasmaDaniel Kolarich
Biochemistry Division, Department of Chemistry, University of Natural Resources and Applied Life Sciences, Vienna, Austria
Proteomics 8:254-63. 2008..This first comprehensive glycoproteomic analysis of the important human plasma glycoprotein BChE did not give any indication of O-glycosylation or any other kind of PTMs as previously postulated...
Discovery and structural characterization of fucosylated oligomannosidic N-glycans in mushroomsJosephine Grass
Department of Chemistry, University of Natural Resources and Life Sciences, 1190 Vienna, Austria
J Biol Chem 286:5977-84. 2011..Although undiscovered so far, L-fucose appears to represent a prominent feature of protein-linked glycans in the fungal kingdom...
Two novel types of O-glycans on the mugwort pollen allergen Art v 1 and their role in antibody bindingRenaud Leonard
Division of Biochemistry, Department of Chemistry, Universitaet fuer Bodenkultur Wien, Muthgasse 18, A-1190 Vienna, Austria
J Biol Chem 280:7932-40. 2005..Mono-beta-arabinosylated hydroxyproline residues thus constitute a new, potentially cross-reactive, carbohydrate determinant in plant proteins...
Mapping of Malus domestica allergens by 2-D electrophoresis and IgE-reactivityAnita Herndl
Plant Biotechnology Unit, Department of Biotechnology, BOKU, Vienna, Austria
Electrophoresis 28:437-48. 2007....
Analysis of immunoglobulin glycosylation by LC-ESI-MS of glycopeptides and oligosaccharidesJohannes Stadlmann
Department of Chemistry, University of Natural Resources and Applied Life Sciences BOKU, Vienna, Austria
Proteomics 8:2858-71. 2008..Regarding fast and yet detailed structural assignment, LC-MS on graphitic carbon supersedes all other current methods...
Comparison of fluorescent labels for oligosaccharides and introduction of a new postlabeling purification methodMartin Pabst
Department of Chemistry, University of Natural Resources and Applied Life Sciences BOKU, 1190 Vienna, Austria
Anal Biochem 384:263-73. 2009....
Structural analysis of the glycoprotein allergen Hev b 4 from natural rubber latex by mass spectrometryDaniel Kolarich
Division of Biochemistry, Department of Chemistry, University of Natural Resources and Applied Life Sciences, Vienna, Austria
Biochim Biophys Acta 1760:715-20. 2006..This study once more demonstrates the outstanding analytical potential of electrospray ionization-mass spectrometry coupled with liquid chromatographic separation...
Plant-based heterologous expression of Mal d 2, a thaumatin-like protein and allergen of apple (Malus domestica), and its characterization as an antifungal proteinMonika Krebitz
Department of Pathophysiology, University of Vienna, AKH-EBO-3Q, Waehringer Guertel 18-20, 1090, Vienna, Austria
J Mol Biol 329:721-30. 2003..In addition, the recombinant thaumatin-like Mal d 2 exhibited antifungal activity against Fusarium oxysporum and Penicillium expansum, implying a function in plant defense against fungal pathogens...
Nitroimidazole action in Entamoeba histolytica: a central role for thioredoxin reductaseDavid Leitsch
Department of Specific Prophylaxis and Tropical Medicine, Center for Physiology and Pathophysiology, Medical University of Vienna, Vienna, Austria
PLoS Biol 5:e211. 2007..Because thioredoxin reductase is a ubiquitous enzyme, similar processes could occur in other eukaryotic or prokaryotic organisms...
Sialic acid concentrations in plants are in the range of inadvertent contaminationReinhard Zeleny
Department of Chemistry, Institute of Biochemistry, , Muthgasse 18, 1190, Vienna, Austria
Planta 224:222-7. 2006....
Carbohydrate moieties can induce mediator release: a detailed characterization of two major timothy grass pollen allergensDaniel Wicklein
Forschungszentrum Borstel, Parkallee 22, D 23845 Borstel, Germany
Biol Chem 385:397-407. 2004..In contrast, the carbohydrate moiety of Phl p 13, which carries four of the same N-glycans (like Phl p 1), can cross-link IgE-receptors via carbohydrate chains and elicits IL-4 release from basophils...
Hazelnut (Corylus avellana) vicilin Cor a 11: molecular characterization of a glycoprotein and its allergenic activityIris Lauer
Department of Allergology, Paul Ehrlich Institut, Langen, Germany
Biochem J 383:327-34. 2004..In conclusion, the hazelnut vicilin Cor a 11 is a minor allergen both in regard to prevalence and allergenic potency, whereas its glycan does not contribute to its allergenic activity...
A genetic and structural analysis of the N-glycosylation capabilitiesRenaud Leonard
Department für Chemie, Universität für Bodenkultur, Muthgasse 18, A 1190 Vienna, Austria
Plant Mol Biol 55:631-44. 2004..2.38) and successfully expressed them in Pichia pastoris. Rice GlcNAc-TI, FucTA and xylosyltransferase are therefore the first monocotyledon glycosyltransferases involved in N-glycan biosynthesis to be characterised in a recombinant form...
N-acetylmuramic acid as capping element of alpha-D-fucose-containing S-layer glycoprotein glycans from Geobacillus tepidamans GS5-97THanspeter Kählig
Department of Organic Chemistry, University of Vienna, Austria
J Biol Chem 280:20292-9. 2005..tepidamans GS5-97(T)...
Unexpected deposition patterns of recombinant proteins in post-endoplasmic reticulum compartments of wheat endospermElsa Arcalis
Institute for Molecular Biotechnology, , 52074 Aachen, Germany
Plant Physiol 136:3457-66. 2004..Our data also confirm that recombinant proteins are invaluable tools for the analysis of protein trafficking in cereals...
Comparative characterisation of recombinant invertebrate and vertebrate peptide O-XylosyltransferasesAndrea Brunner
Department für Chemie der, Universität für Bodenkultur, Muthgasse 18, A 1190, Wien, Austria
Glycoconj J 23:543-54. 2006..Using this substrate, we could for the first time follow, by mass spectrometry, the xylosylation of a peptide with multiple xylosyltransferase acceptor motifs...
Distal side tryptophan, tyrosine and methionine in catalase-peroxidases are covalently linked in solutionChrista Jakopitsch
Institute of Chemistry, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
FEBS Lett 552:135-40. 2003..It is proposed that the covalent bond between Trp and Tyr may form before that between Tyr and Met. The findings are discussed with respect to the mechanism of cross-linking and its role in KatG catalysis...
Arabidopsis thaliana beta1,2-xylosyltransferase: an unusual glycosyltransferase with the potential to act at multiple stages of the plant N-glycosylation pathwayPeter Bencur
Department für Angewandte Pflanzenwissenschaften und Pflanzenbiotechnologie, Institut für Angewandte Genetik und Zellbiologie, Universitat fur Bodenkultur Wien, Muthgasse 18, A 1190 Wien, Austria
Biochem J 388:515-25. 2005..These experiments revealed that the substrate specificity of XylT permits the enzyme to act at multiple stages of the plant N-glycosylation pathway...
