Research Topics
Genomes and Genes | Sepp D KohlweinSummaryAffiliation: University of Graz Country: Austria Publications
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Publications
Cdk1/Cdc28-dependent activation of the major triacylglycerol lipase Tgl4 in yeast links lipolysis to cell-cycle progressionChristoph F Kurat
Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50 II, A8010 Graz, Austria
Mol Cell 33:53-63. 2009..Our data provide evidence for a direct link between cell-cycle-regulatory kinases and TG degradation and suggest a general mechanism for coordinating membrane synthesis with cell-cycle progression...
Good fat, essential cellular requirements for triacylglycerol synthesis to maintain membrane homeostasis in yeastJulia Petschnigg
Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50 II, A8010 Graz, Austria
J Biol Chem 284:30981-93. 2009....- Lipid droplets and peroxisomes: key players in cellular lipid homeostasis or a matter of fat--store 'em up or burn 'em downSepp D Kohlwein
Institute of Molecular Biosciences, University of Graz, 8010 Graz, Austria
Genetics 193:1-50. 2013..These questions are particularly burning in view of the pandemic development of lipid-associated disorders worldwide... 
SLipid-induced cell dysfunction and cell death: lessons from yeastSepp D Kohlwein
Institut für Molekulare Biowissenschaften, Universitat Graz, Humboldtstrasse 50, A8010 Graz, Austria
Curr Hypertens Rep 9:455-61. 2007..This article reviews recently uncovered aspects of fatty acid-associated malfunctions and lipotoxicity in yeast that may aid in understanding the molecular basis of lipid-associated disorders in mammals...- YPL.db2: the Yeast Protein Localization database, version 2.0Mathias Kals
SFB Biomembrane Research Center, Institute of Molecular Biosciences, University of Graz, Schubertstrasse 1, A8010 Graz, Austria
Yeast 22:213-8. 2005..Access to the Yeast Protein Localization database version 2.0 (YPL.db(2)) is available through http://YPL.uni-graz.at... - Triacylglycerol homeostasis: insights from yeastSepp D Kohlwein
Institute of Molecular Biosciences, University of Graz, A 8010 Graz, Austria
J Biol Chem 285:15663-7. 2010..Based on these similarities, studies performed in yeast may provide further significant mechanistic insight into the molecular basis of triacylglycerol homeostasis and its important physiological roles in healthy and diseased cells... - Obese and anorexic yeasts: experimental models to understand the metabolic syndrome and lipotoxicitySepp D Kohlwein
Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50 II, A8010 Graz, Austria
Biochim Biophys Acta 1801:222-9. 2010.... - Obese yeast: triglyceride lipolysis is functionally conserved from mammals to yeastChristoph F Kurat
Institute of Molecular Biosciences, University of Graz, Schubertstrasse 1, A8010 Graz, Austria
J Biol Chem 281:491-500. 2006..Our data identify yeast Tgl4 as a functional ortholog of mammalian adipose triglyceride lipase... - Imaging-based live cell yeast screen identifies novel factors involved in peroxisome assemblyHeimo Wolinski
Institute of Molecular Biosciences, University of Graz, Austria
J Proteome Res 8:20-7. 2009.... - S-adenosyl-L-homocysteine hydrolase, key enzyme of methylation metabolism, regulates phosphatidylcholine synthesis and triacylglycerol homeostasis in yeast: implications for homocysteine as a risk factor of atherosclerosisNermina Malanovic
Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50 II, Graz, Austria
J Biol Chem 283:23989-99. 2008..The addition of homocysteine, by the reversal of the Sah1-catalyzed reaction, also leads to TG accumulation in yeast, providing an attractive model for the role of homocysteine as a risk factor of atherosclerosis in humans... - Identification of Yju3p as functional orthologue of mammalian monoglyceride lipase in the yeast SaccharomycescerevisiaeChristoph Heier
Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50, A 8010 Graz, Austria
Biochim Biophys Acta 1801:1063-71. 2010..In conclusion, our studies suggest that catabolic and anabolic reactions affect cellular MAG levels. Yju3p is the functional orthologue of mammalian MGL and is required for efficient degradation of MAG in yeast... - The N-terminal region of comparative gene identification-58 (CGI-58) is important for lipid droplet binding and activation of adipose triglyceride lipaseAstrid Gruber
Institute of Molecular Biosciences, University of Graz, Humboldtstrasse, A 8010 Graz, Austria
J Biol Chem 285:12289-98. 2010..In summary, our study shows that the N-terminal, Trp-rich region of CGI-58 is essential for correct localization and ATGL-activating function of CGI-58... - Quantitative modeling of triacylglycerol homeostasis in yeast--metabolic requirement for lipolysis to promote membrane lipid synthesis and cellular growthJürgen Zanghellini
Institute of Chemistry, University of Graz, Austria
FEBS J 275:5552-63. 2008..Progress in triacylglycerol hydrolysis directly correlates with an increase in cell size, demonstrating the importance of lipolysis for supporting efficient growth initiation... - Identification of a cardiolipin-specific phospholipase encoded by the gene CLD1 (YGR110W) in yeastAndreas Beranek
Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50 II, Graz A 8010, Austria
J Biol Chem 284:11572-8. 2009..We therefore rename the Ygr110wp as Cld1p (cardiolipin-specific deacylase 1)... - The spatial organization of lipid synthesis in the yeast Saccharomyces cerevisiae derived from large scale green fluorescent protein tagging and high resolution microscopyKlaus Natter
Institute of Molecular Biosciences, Spezialforschungsbereich Biomembrane Research Center, University of Graz, Schubertstr. 1, A8010 Graz, Austria
Mol Cell Proteomics 4:662-72. 2005.... - The lipid droplet enzyme Tgl1p hydrolyzes both steryl esters and triglycerides in the yeast, Saccharomyces cerevisiaeAnita Jandrositz
Institute of Molecular Biosciences, SFB Biomembrane Research Center, University of Graz, Schubertstr 1, A8010 Graz, Austria
Biochim Biophys Acta 1735:50-8. 2005..cerevisiae. In a tgl1 deletion mutant, the mobilization of steryl esters in vivo was delayed, but not abolished, suggesting the existence of additional enzymes involved in steryl ester mobilization... - Fatty acid synthesis and elongation in yeastOksana Tehlivets
Institute of Molecular Biosciences, University of Graz, A8010 Graz, Austria
Biochim Biophys Acta 1771:255-70. 2007..In this review, we summarize what is currently known about enzymes involved in cellular fatty acid synthesis and elongation, and discuss potential links between fatty acid metabolism, physiology and cellular regulation... - The fidgety yeast: focus on high-resolution live yeast cell microscopyHeimo Wolinski
Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50 II, A8010, Graz, Austria
Methods Mol Biol 548:75-99. 2009..If an image tells more than a thousand (perhaps misleading?) words, the ease of obtaining "images" thus rather suggests analyzing many thousands of images, to come up with one relevant and biologically significant conclusion... - YPL.db: the Yeast Protein Localization databaseGeorg Habeler
Institute of Biomedical Engineering, Graz University of Technology, Krenngasse 37, 8010 Graz, Austria
Nucleic Acids Res 30:80-3. 2002..Links to MIPS, YPD and SGD databases are provided to allow fast access to further information not contained in the localization database itself. YPL.db is available at http://ypl.tugraz.at... - FAT SIGNALS--lipases and lipolysis in lipid metabolism and signalingRudolf Zechner
Institute of Molecular Biosciences, University of Graz, Austria
Cell Metab 15:279-91. 2012.... - Identification of an insulin-regulated lysophospholipase with homology to neuropathy target esterasePetra C Kienesberger
Institute of Molecular Biosciences, University of Graz 8010, Graz, Austria
J Biol Chem 283:5908-17. 2008..Regulation of enzyme expression by the nutritional status and insulin suggests a role of NRE in the catabolism of lipid precursors and/or mediators that affect energy metabolism in mammals... - Fatty acids trigger mitochondrion-dependent necrosisPatrick Rockenfeller
University of Graz, Institute for Molecular Biosciences, Austria
Cell Cycle 9:2836-42. 2010..We conclude that lipotoxicity is executed via a mitochondrial necrotic pathway, challenging the dogma that the adverse effects of lipid stress are exclusively apoptotic... - Microscopic analysis of lipid droplet metabolism and dynamics in yeastHeimo Wolinski
Institute of Molecular Biosciences, University of Graz, Graz, Austria
Methods Mol Biol 457:151-63. 2008..The applications and pitfalls of such techniques in understanding lipid storage and degradation are discussed... - Remodeling of lipid droplets during lipolysis and growth in adipocytesMargret Paar
Institute of Molecular Biosciences, Lipidomics Research Center LRC Graz, University of Graz, 8010 Graz, Austria
J Biol Chem 287:11164-73. 2012..Furthermore, we propose that formation of large LDs requires a yet uncharacterized protein machinery mediating LD interaction and lipid transfer... - A role for seipin in lipid droplet dynamics and inheritance in yeastHeimo Wolinski
Institute of Molecular Biosciences, University of Graz, Humboldtstr 50 II, 8010 Graz, Austria
J Cell Sci 124:3894-904. 2011.... - Esterase 22 and beta-glucuronidase hydrolyze retinoids in mouse liverRenate Schreiber
Institute of Molecular Biosciences, University of Graz, 8010 Graz, Austria
J Lipid Res 50:2514-23. 2009..Interestingly, however, Gus was capable of hydrolyzing the naturally occurring vitamin A metabolite retinoyl beta-glucuronide. In conclusion, our observations implicate that both Es22 and Gus play a role in liver retinoid metabolism... - Quantitative imaging of lipid metabolism in yeast: from 4D analysis to high content screens of mutant librariesHeimo Wolinski
Institute of Molecular Biosciences, University of Graz, Graz, Austria
Methods Cell Biol 108:345-65. 2012..Also, implementation of coherent anti-Stokes Raman scattering (CARS) microscopy as an emerging tool for label-free lipid imaging in living cells will be discussed... - Nanopatterned polymer substrates promote endothelial proliferation by initiation of β-catenin transcriptional signalingMichaela Schernthaner
Department of Pharmacology and Toxicology, University of Graz, A 8010 Graz, Austria
Acta Biomater 8:2953-62. 2012..In summary, we present a novel mechanism of surface topology-dependent control of transcriptional programs in mature endothelium and endothelial progenitor cells... - Lipid-dependent subcellular relocalization of the acyl chain desaturase in yeastVerena Tatzer
Department of Biochemistry, Graz University of Technology, A 8010 Graz, Austria
Mol Biol Cell 13:4429-42. 2002..A model is discussed in which the accumulation of saturated lipids in a microdomain around the desaturase could induce the observed segregation and relocalization of the enzyme... - Extracellular nucleases and extracellular DNA play important roles in Vibrio cholerae biofilm formationAndrea Seper
Institut fuer Molekulare Biowissenschaften, Karl Franzens Universitaet Graz, Humboldtstrasse 50, 8010 Graz, Austria
Mol Microbiol 82:1015-37. 2011..This study provides new insights into biofilm development and transmission of biofilm-derived V. cholerae... - S-adenosyl-L-homocysteine hydrolase in yeast: key enzyme of methylation metabolism and coordinated regulation with phospholipid synthesisOksana Tehlivets
Institute of Molecular Biosciences, SFB Biomembrane Research Center, University of Graz, Schubertstrasse 1, 8010 Graz, Austria
FEBS Lett 577:501-6. 2004..Profound changes in cellular lipid composition upon depletion of Sah1p support the notion of a tight interaction between lipid metabolism and Sah1p function... - Cellular cholesterol controls TRPC3 function: evidence from a novel dominant-negative knockdown strategyAnnarita Graziani
Institute of Pharmaceutical Sciences, Pharmacology and Toxicology, Karl Franzens University of Graz, Universitaetsplatz 2, A 8010 Graz, Austria
Biochem J 396:147-55. 2006..We suggest the cholesterol content of the plasma membrane as a determinant of cellular TRPC3 activity and provide evidence for cholesterol dependence of TRPC3 surface expression... - An automated approach for three-dimensional quantification of fibrillar structures in optically cleared soft biological tissuesAndreas J Schriefl
Institute of Biomechanics, Center of Biomedical Engineering, Graz University of Technology, Kronesgasse 5 I, 8010 Graz, Austria
J R Soc Interface 10:20120760. 2013.... - Yeast and cancer cells - common principles in lipid metabolismKlaus Natter
University of Graz, Institute of Molecular Biosciences, Lipidomics Research Center Graz, Humboldtstrasse 50 II, 8010 Graz
Biochim Biophys Acta 1831:314-26. 2013..Thus, understanding lipid metabolism in S. cerevisiae during cell cycle progression and cell proliferation may complement recent efforts to understand the importance and fundamental regulatory mechanisms of these pathways in cancer... - Nutritional requirements of the BY series of Saccharomyces cerevisiae strains for optimum growthMichael Hanscho
Institute of Molecular Biosciences, University Graz, Graz, Austria
FEMS Yeast Res 12:796-808. 2012..Thus, several of the existing growth media recipes need to be revised to achieve optimum growth conditions for BY-derived strains... - Vascular bioactivation of nitroglycerin is catalyzed by cytosolic aldehyde dehydrogenase-2Matteo Beretta
Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Austria
Circ Res 110:385-93. 2012..Despite having wide implications to GTN pharmacology and raising many questions that are still unresolved, mitochondrial bioactivation of GTN in blood vessels is still lacking experimental support... - Yeast oxidosqualene cyclase (Erg7p) is a major component of lipid particlesPaola Milla
Dipartimento di Scienza e Tecnologia del Farmaco, , , Corso Raffaello 31, I-10125 Torino, Italy
J Biol Chem 277:2406-12. 2002.... - The Saccharomyces cerevisiae YBR159w gene encodes the 3-ketoreductase of the microsomal fatty acid elongaseGongshe Han
Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20184, USA
J Biol Chem 277:35440-9. 2002..By disrupting the orthologs of Ybr159w in the ybr159Delta mutant we found that the ybr159Deltaayr1Delta double mutant was inviable, suggesting that Ayr1p is responsible for the residual 3-ketoreductase activity... - Mitochondrial phosphate-carrier deficiency: a novel disorder of oxidative phosphorylationJohannes A Mayr
Department of Pediatrics, Paracelsus Private Medical University, Salzburg, Austria
Am J Hum Genet 80:478-84. 2007..The enzyme defect was confirmed by complementation analysis in yeast. This is the first report of patients with mitochondrial phosphate-carrier deficiency... - A block in endoplasmic reticulum-to-Golgi trafficking inhibits phospholipid synthesis and induces neutral lipid accumulationMaria L Gaspar
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
J Biol Chem 283:25735-51. 2008..We conclude that this metabolic switch provides a degree of protection to cells during secretory stress... - Saccharomyces cerevisiae strain expressing a plant fatty acid desaturase produces polyunsaturated fatty acids and is susceptible to oxidative stress induced by lipid peroxidationAna Cipak
Institute Rudjer Boskovic, Zagreb, Croatia
Free Radic Biol Med 40:897-906. 2006..Its potential to investigate the molecular basis of responses to oxidative stress, in particular the involvement of reactive aldehydes derived from fatty acid peroxidation, especially 4-hydroxy-2-nonenal, will be addressed... - Sterol binding assay using surface plasmon fluorescence spectroscopyBirgit Wiltschi
Department of Membrane Biochemistry, Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
Anal Chem 78:547-55. 2006..With the design of our novel in vitro oxysterol binding assay, we have solved the technically challenging difficulty of presenting hydrophobic ligands to hydrophilic proteins in aqueous media... - Subcellular localization of yeast Sec14 homologues and their involvement in regulation of phospholipid turnoverMartina Schnabl
Department of Biochemistry, University of Technology, Graz, Austria
Eur J Biochem 270:3133-45. 2003..In summary, Sfh2p and Sfh4p are the Sec14p homologues with the most pronounced functional similarity to Sec14p, whereas the other Sfh proteins appear to be functionally less related to Sec14p... - Interaction of Pik1p and Sjl proteins in membrane traffickingPeter H Nguyen
Department of Biological Sciences, St John s University, 8000 Utopia Parkway, Jamaica, NY 11439, USA
FEMS Yeast Res 5:363-71. 2005.... - The role of respiration, reactive oxygen species and oxidative stress in mother cell-specific ageing of yeast strains defective in the RAS signalling pathwayGino Heeren
Department of Cell Biology, University of Salzburg, Hellbrunnerstrasse 34, A-5020 Salzburg, Austria
FEMS Yeast Res 5:157-67. 2004..Our results indicate that oxidative stress resulting from a disturbance of redox balance can play a major role in mother cell-specific lifespan determination of yeast cells... - Characterization, localization and functional analysis of Gpr1p, a protein affecting sensitivity to acetic acid in the yeast Yarrowia lipolyticaAntje Augstein
Institute of Microbiology, Dresden University of Technology, Mommsenstrasse 13, D-01062 Dresden, Germany
Microbiology 149:589-600. 2003..Fluorescence microscopy confirmed a localization to the plasma membrane. A model is presented describing a hypothetical function of Gpr1p during adaptation to acetic acid... - Adaptation to oxidative stress induced by polyunsaturated fatty acids in yeastAna Cipak
Rudjer Boskovic Institute, Zagreb, Croatia
Biochim Biophys Acta 1781:283-7. 2008..These data demonstrate that the cellular synthesis of PUFA induces endogenous oxidative stress which is overcome by cellular adaptation based on the catalase activity...