Sepp D Kohlwein

Summary

Affiliation: University of Graz
Country: Austria

Publications

  1. doi request reprint Cdk1/Cdc28-dependent activation of the major triacylglycerol lipase Tgl4 in yeast links lipolysis to cell-cycle progression
    Christoph F Kurat
    Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50 II, A8010 Graz, Austria
    Mol Cell 33:53-63. 2009
  2. pmc Good fat, essential cellular requirements for triacylglycerol synthesis to maintain membrane homeostasis in yeast
    Julia Petschnigg
    Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50 II, A8010 Graz, Austria
    J Biol Chem 284:30981-93. 2009
  3. pmc Lipid droplets and peroxisomes: key players in cellular lipid homeostasis or a matter of fat--store 'em up or burn 'em down
    Sepp D Kohlwein
    Institute of Molecular Biosciences, University of Graz, 8010 Graz, Austria
    Genetics 193:1-50. 2013
  4. doi request reprint Obese and anorexic yeasts: experimental models to understand the metabolic syndrome and lipotoxicity
    Sepp D Kohlwein
    Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50 II, A8010 Graz, Austria
    Biochim Biophys Acta 1801:222-9. 2010
  5. ncbi request reprint SLipid-induced cell dysfunction and cell death: lessons from yeast
    Sepp D Kohlwein
    Institut für Molekulare Biowissenschaften, Universitat Graz, Humboldtstrasse 50, A8010 Graz, Austria
    Curr Hypertens Rep 9:455-61. 2007
  6. pmc Triacylglycerol homeostasis: insights from yeast
    Sepp D Kohlwein
    Institute of Molecular Biosciences, University of Graz, A 8010 Graz, Austria
    J Biol Chem 285:15663-7. 2010
  7. ncbi request reprint YPL.db2: the Yeast Protein Localization database, version 2.0
    Mathias Kals
    SFB Biomembrane Research Center, Institute of Molecular Biosciences, University of Graz, Schubertstrasse 1, A8010 Graz, Austria
    Yeast 22:213-8. 2005
  8. ncbi request reprint Obese yeast: triglyceride lipolysis is functionally conserved from mammals to yeast
    Christoph F Kurat
    Institute of Molecular Biosciences, University of Graz, Schubertstrasse 1, A8010 Graz, Austria
    J Biol Chem 281:491-500. 2006
  9. pmc S-adenosyl-L-homocysteine hydrolase, key enzyme of methylation metabolism, regulates phosphatidylcholine synthesis and triacylglycerol homeostasis in yeast: implications for homocysteine as a risk factor of atherosclerosis
    Nermina Malanovic
    Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50 II, Graz, Austria
    J Biol Chem 283:23989-99. 2008
  10. doi request reprint Imaging-based live cell yeast screen identifies novel factors involved in peroxisome assembly
    Heimo Wolinski
    Institute of Molecular Biosciences, University of Graz, Austria
    J Proteome Res 8:20-7. 2009

Collaborators

Detail Information

Publications49

  1. doi request reprint Cdk1/Cdc28-dependent activation of the major triacylglycerol lipase Tgl4 in yeast links lipolysis to cell-cycle progression
    Christoph F Kurat
    Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50 II, A8010 Graz, Austria
    Mol Cell 33:53-63. 2009
    ..Our data provide evidence for a direct link between cell-cycle-regulatory kinases and TG degradation and suggest a general mechanism for coordinating membrane synthesis with cell-cycle progression...
  2. pmc Good fat, essential cellular requirements for triacylglycerol synthesis to maintain membrane homeostasis in yeast
    Julia Petschnigg
    Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50 II, A8010 Graz, Austria
    J Biol Chem 284:30981-93. 2009
    ....
  3. pmc Lipid droplets and peroxisomes: key players in cellular lipid homeostasis or a matter of fat--store 'em up or burn 'em down
    Sepp D Kohlwein
    Institute of Molecular Biosciences, University of Graz, 8010 Graz, Austria
    Genetics 193:1-50. 2013
    ..These questions are particularly burning in view of the pandemic development of lipid-associated disorders worldwide...
  4. doi request reprint Obese and anorexic yeasts: experimental models to understand the metabolic syndrome and lipotoxicity
    Sepp D Kohlwein
    Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50 II, A8010 Graz, Austria
    Biochim Biophys Acta 1801:222-9. 2010
    ....
  5. ncbi request reprint SLipid-induced cell dysfunction and cell death: lessons from yeast
    Sepp D Kohlwein
    Institut für Molekulare Biowissenschaften, Universitat Graz, Humboldtstrasse 50, A8010 Graz, Austria
    Curr Hypertens Rep 9:455-61. 2007
    ..This article reviews recently uncovered aspects of fatty acid-associated malfunctions and lipotoxicity in yeast that may aid in understanding the molecular basis of lipid-associated disorders in mammals...
  6. pmc Triacylglycerol homeostasis: insights from yeast
    Sepp D Kohlwein
    Institute of Molecular Biosciences, University of Graz, A 8010 Graz, Austria
    J Biol Chem 285:15663-7. 2010
    ..Based on these similarities, studies performed in yeast may provide further significant mechanistic insight into the molecular basis of triacylglycerol homeostasis and its important physiological roles in healthy and diseased cells...
  7. ncbi request reprint YPL.db2: the Yeast Protein Localization database, version 2.0
    Mathias Kals
    SFB Biomembrane Research Center, Institute of Molecular Biosciences, University of Graz, Schubertstrasse 1, A8010 Graz, Austria
    Yeast 22:213-8. 2005
    ..Access to the Yeast Protein Localization database version 2.0 (YPL.db(2)) is available through http://YPL.uni-graz.at...
  8. ncbi request reprint Obese yeast: triglyceride lipolysis is functionally conserved from mammals to yeast
    Christoph F Kurat
    Institute of Molecular Biosciences, University of Graz, Schubertstrasse 1, A8010 Graz, Austria
    J Biol Chem 281:491-500. 2006
    ..Our data identify yeast Tgl4 as a functional ortholog of mammalian adipose triglyceride lipase...
  9. pmc S-adenosyl-L-homocysteine hydrolase, key enzyme of methylation metabolism, regulates phosphatidylcholine synthesis and triacylglycerol homeostasis in yeast: implications for homocysteine as a risk factor of atherosclerosis
    Nermina Malanovic
    Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50 II, Graz, Austria
    J Biol Chem 283:23989-99. 2008
    ..The addition of homocysteine, by the reversal of the Sah1-catalyzed reaction, also leads to TG accumulation in yeast, providing an attractive model for the role of homocysteine as a risk factor of atherosclerosis in humans...
  10. doi request reprint Imaging-based live cell yeast screen identifies novel factors involved in peroxisome assembly
    Heimo Wolinski
    Institute of Molecular Biosciences, University of Graz, Austria
    J Proteome Res 8:20-7. 2009
    ....
  11. pmc Identification of Yju3p as functional orthologue of mammalian monoglyceride lipase in the yeast Saccharomycescerevisiae
    Christoph Heier
    Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50, A 8010 Graz, Austria
    Biochim Biophys Acta 1801:1063-71. 2010
    ..In conclusion, our studies suggest that catabolic and anabolic reactions affect cellular MAG levels. Yju3p is the functional orthologue of mammalian MGL and is required for efficient degradation of MAG in yeast...
  12. pmc Identification of a cardiolipin-specific phospholipase encoded by the gene CLD1 (YGR110W) in yeast
    Andreas Beranek
    Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50 II, Graz A 8010, Austria
    J Biol Chem 284:11572-8. 2009
    ..We therefore rename the Ygr110wp as Cld1p (cardiolipin-specific deacylase 1)...
  13. pmc The N-terminal region of comparative gene identification-58 (CGI-58) is important for lipid droplet binding and activation of adipose triglyceride lipase
    Astrid Gruber
    Institute of Molecular Biosciences, University of Graz, Humboldtstrasse, A 8010 Graz, Austria
    J Biol Chem 285:12289-98. 2010
    ..In summary, our study shows that the N-terminal, Trp-rich region of CGI-58 is essential for correct localization and ATGL-activating function of CGI-58...
  14. ncbi request reprint The spatial organization of lipid synthesis in the yeast Saccharomyces cerevisiae derived from large scale green fluorescent protein tagging and high resolution microscopy
    Klaus Natter
    Institute of Molecular Biosciences, Spezialforschungsbereich Biomembrane Research Center, University of Graz, Schubertstr 1, A8010 Graz, Austria
    Mol Cell Proteomics 4:662-72. 2005
    ....
  15. doi request reprint Quantitative modeling of triacylglycerol homeostasis in yeast--metabolic requirement for lipolysis to promote membrane lipid synthesis and cellular growth
    Jürgen Zanghellini
    Institute of Chemistry, University of Graz, Austria
    FEBS J 275:5552-63. 2008
    ..Progress in triacylglycerol hydrolysis directly correlates with an increase in cell size, demonstrating the importance of lipolysis for supporting efficient growth initiation...
  16. ncbi request reprint The lipid droplet enzyme Tgl1p hydrolyzes both steryl esters and triglycerides in the yeast, Saccharomyces cerevisiae
    Anita Jandrositz
    Institute of Molecular Biosciences, SFB Biomembrane Research Center, University of Graz, Schubertstr 1, A8010 Graz, Austria
    Biochim Biophys Acta 1735:50-8. 2005
    ..cerevisiae. In a tgl1 deletion mutant, the mobilization of steryl esters in vivo was delayed, but not abolished, suggesting the existence of additional enzymes involved in steryl ester mobilization...
  17. doi request reprint The fidgety yeast: focus on high-resolution live yeast cell microscopy
    Heimo Wolinski
    Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50 II, A8010, Graz, Austria
    Methods Mol Biol 548:75-99. 2009
    ..If an image tells more than a thousand (perhaps misleading?) words, the ease of obtaining "images" thus rather suggests analyzing many thousands of images, to come up with one relevant and biologically significant conclusion...
  18. ncbi request reprint Fatty acid synthesis and elongation in yeast
    Oksana Tehlivets
    Institute of Molecular Biosciences, University of Graz, A8010 Graz, Austria
    Biochim Biophys Acta 1771:255-70. 2007
    ..In this review, we summarize what is currently known about enzymes involved in cellular fatty acid synthesis and elongation, and discuss potential links between fatty acid metabolism, physiology and cellular regulation...
  19. pmc YPL.db: the Yeast Protein Localization database
    Georg Habeler
    Institute of Biomedical Engineering, Graz University of Technology, Krenngasse 37, 8010 Graz, Austria
    Nucleic Acids Res 30:80-3. 2002
    ..Links to MIPS, YPD and SGD databases are provided to allow fast access to further information not contained in the localization database itself. YPL.db is available at http://ypl.tugraz.at...
  20. pmc FAT SIGNALS--lipases and lipolysis in lipid metabolism and signaling
    Rudolf Zechner
    Institute of Molecular Biosciences, University of Graz, Austria
    Cell Metab 15:279-91. 2012
    ....
  21. ncbi request reprint Microscopic analysis of lipid droplet metabolism and dynamics in yeast
    Heimo Wolinski
    Institute of Molecular Biosciences, University of Graz, Graz, Austria
    Methods Mol Biol 457:151-63. 2008
    ..The applications and pitfalls of such techniques in understanding lipid storage and degradation are discussed...
  22. ncbi request reprint Fatty acids trigger mitochondrion-dependent necrosis
    Patrick Rockenfeller
    University of Graz, Institute for Molecular Biosciences, Austria
    Cell Cycle 9:2836-42. 2010
    ..We conclude that lipotoxicity is executed via a mitochondrial necrotic pathway, challenging the dogma that the adverse effects of lipid stress are exclusively apoptotic...
  23. ncbi request reprint Identification of an insulin-regulated lysophospholipase with homology to neuropathy target esterase
    Petra C Kienesberger
    Institute of Molecular Biosciences, University of Graz 8010, Graz, Austria
    J Biol Chem 283:5908-17. 2008
    ..Regulation of enzyme expression by the nutritional status and insulin suggests a role of NRE in the catabolism of lipid precursors and/or mediators that affect energy metabolism in mammals...
  24. pmc Yeast and cancer cells - common principles in lipid metabolism
    Klaus Natter
    University of Graz, Institute of Molecular Biosciences, Lipidomics Research Center Graz, Humboldtstrasse 50 II, 8010 Graz
    Biochim Biophys Acta 1831:314-26. 2013
    ..Thus, understanding lipid metabolism in S. cerevisiae during cell cycle progression and cell proliferation may complement recent efforts to understand the importance and fundamental regulatory mechanisms of these pathways in cancer...
  25. pmc Remodeling of lipid droplets during lipolysis and growth in adipocytes
    Margret Paar
    Institute of Molecular Biosciences, Lipidomics Research Center LRC Graz, University of Graz, 8010 Graz, Austria
    J Biol Chem 287:11164-73. 2012
    ..Furthermore, we propose that formation of large LDs requires a yet uncharacterized protein machinery mediating LD interaction and lipid transfer...
  26. doi request reprint A role for seipin in lipid droplet dynamics and inheritance in yeast
    Heimo Wolinski
    Institute of Molecular Biosciences, University of Graz, Humboldtstr 50 II, 8010 Graz, Austria
    J Cell Sci 124:3894-904. 2011
    ....
  27. pmc Esterase 22 and beta-glucuronidase hydrolyze retinoids in mouse liver
    Renate Schreiber
    Institute of Molecular Biosciences, University of Graz, 8010 Graz, Austria
    J Lipid Res 50:2514-23. 2009
    ..Interestingly, however, Gus was capable of hydrolyzing the naturally occurring vitamin A metabolite retinoyl beta-glucuronide. In conclusion, our observations implicate that both Es22 and Gus play a role in liver retinoid metabolism...
  28. doi request reprint Lipid droplet autophagy in the yeast Saccharomyces cerevisiae
    Tim van Zutphen
    Molecular Cell Biology, University of Groningen, 9747 AG Groningen, Netherlands Institute of Molecular Biosciences, University of Graz, 8010 Graz, Austria
    Mol Biol Cell 25:290-301. 2014
    ..Furthermore, none of the core autophagy proteins, including Atg1 and Atg8, is required for LD formation in yeast. ..
  29. doi request reprint Quantitative imaging of lipid metabolism in yeast: from 4D analysis to high content screens of mutant libraries
    Heimo Wolinski
    Institute of Molecular Biosciences, University of Graz, Graz, Austria
    Methods Cell Biol 108:345-65. 2012
    ..Also, implementation of coherent anti-Stokes Raman scattering (CARS) microscopy as an emerging tool for label-free lipid imaging in living cells will be discussed...
  30. doi request reprint Nutritional requirements of the BY series of Saccharomyces cerevisiae strains for optimum growth
    Michael Hanscho
    Institute of Molecular Biosciences, University Graz, Graz, Austria
    FEMS Yeast Res 12:796-808. 2012
    ..Thus, several of the existing growth media recipes need to be revised to achieve optimum growth conditions for BY-derived strains...
  31. doi request reprint Nanopatterned polymer substrates promote endothelial proliferation by initiation of β-catenin transcriptional signaling
    Michaela Schernthaner
    Department of Pharmacology and Toxicology, University of Graz, A 8010 Graz, Austria
    Acta Biomater 8:2953-62. 2012
    ..In summary, we present a novel mechanism of surface topology-dependent control of transcriptional programs in mature endothelium and endothelial progenitor cells...
  32. pmc The emergence of lipid droplets in yeast: current status and experimental approaches
    Maja Radulovic
    Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50 II, 8010, Graz, Austria
    Curr Genet 59:231-42. 2013
    ....
  33. pmc Lipid-dependent subcellular relocalization of the acyl chain desaturase in yeast
    Verena Tatzer
    Department of Biochemistry, Graz University of Technology, A 8010 Graz, Austria
    Mol Biol Cell 13:4429-42. 2002
    ..A model is discussed in which the accumulation of saturated lipids in a microdomain around the desaturase could induce the observed segregation and relocalization of the enzyme...
  34. pmc Extracellular nucleases and extracellular DNA play important roles in Vibrio cholerae biofilm formation
    Andrea Seper
    Institut fuer Molekulare Biowissenschaften, Karl Franzens Universitaet Graz, Humboldtstrasse 50, 8010 Graz, Austria
    Mol Microbiol 82:1015-37. 2011
    ..This study provides new insights into biofilm development and transmission of biofilm-derived V. cholerae...
  35. pmc Cellular cholesterol controls TRPC3 function: evidence from a novel dominant-negative knockdown strategy
    Annarita Graziani
    Institute of Pharmaceutical Sciences, Pharmacology and Toxicology, Karl Franzens University of Graz, Universitaetsplatz 2, A 8010 Graz, Austria
    Biochem J 396:147-55. 2006
    ..We suggest the cholesterol content of the plasma membrane as a determinant of cellular TRPC3 activity and provide evidence for cholesterol dependence of TRPC3 surface expression...
  36. ncbi request reprint S-adenosyl-L-homocysteine hydrolase in yeast: key enzyme of methylation metabolism and coordinated regulation with phospholipid synthesis
    Oksana Tehlivets
    Institute of Molecular Biosciences, SFB Biomembrane Research Center, University of Graz, Schubertstrasse 1, 8010 Graz, Austria
    FEBS Lett 577:501-6. 2004
    ..Profound changes in cellular lipid composition upon depletion of Sah1p support the notion of a tight interaction between lipid metabolism and Sah1p function...
  37. pmc An automated approach for three-dimensional quantification of fibrillar structures in optically cleared soft biological tissues
    Andreas J Schriefl
    Institute of Biomechanics, Center of Biomedical Engineering, Graz University of Technology, Kronesgasse 5 I, 8010 Graz, Austria
    J R Soc Interface 10:20120760. 2013
    ....
  38. doi request reprint Vascular bioactivation of nitroglycerin is catalyzed by cytosolic aldehyde dehydrogenase-2
    Matteo Beretta
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Austria
    Circ Res 110:385-93. 2012
    ..Despite having wide implications to GTN pharmacology and raising many questions that are still unresolved, mitochondrial bioactivation of GTN in blood vessels is still lacking experimental support...
  39. ncbi request reprint Yeast oxidosqualene cyclase (Erg7p) is a major component of lipid particles
    Paola Milla
    Dipartimento di Scienza e Tecnologia del Farmaco, Facolta di Farmacia, Universita degli Studi di Torino, Corso Raffaello 31, I 10125 Torino, Italy
    J Biol Chem 277:2406-12. 2002
    ....
  40. ncbi request reprint Saccharomyces cerevisiae strain expressing a plant fatty acid desaturase produces polyunsaturated fatty acids and is susceptible to oxidative stress induced by lipid peroxidation
    Ana Cipak
    Institute Rudjer Boskovic, Zagreb, Croatia
    Free Radic Biol Med 40:897-906. 2006
    ..Its potential to investigate the molecular basis of responses to oxidative stress, in particular the involvement of reactive aldehydes derived from fatty acid peroxidation, especially 4-hydroxy-2-nonenal, will be addressed...
  41. pmc A block in endoplasmic reticulum-to-Golgi trafficking inhibits phospholipid synthesis and induces neutral lipid accumulation
    Maria L Gaspar
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    J Biol Chem 283:25735-51. 2008
    ..We conclude that this metabolic switch provides a degree of protection to cells during secretory stress...
  42. ncbi request reprint The Saccharomyces cerevisiae YBR159w gene encodes the 3-ketoreductase of the microsomal fatty acid elongase
    Gongshe Han
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20184, USA
    J Biol Chem 277:35440-9. 2002
    ..By disrupting the orthologs of Ybr159w in the ybr159Delta mutant we found that the ybr159Deltaayr1Delta double mutant was inviable, suggesting that Ayr1p is responsible for the residual 3-ketoreductase activity...
  43. doi request reprint Adaptation to oxidative stress induced by polyunsaturated fatty acids in yeast
    Ana Cipak
    Rudjer Boskovic Institute, Zagreb, Croatia
    Biochim Biophys Acta 1781:283-7. 2008
    ..These data demonstrate that the cellular synthesis of PUFA induces endogenous oxidative stress which is overcome by cellular adaptation based on the catalase activity...
  44. ncbi request reprint Characterization, localization and functional analysis of Gpr1p, a protein affecting sensitivity to acetic acid in the yeast Yarrowia lipolytica
    Antje Augstein
    Institute of Microbiology, Dresden University of Technology, Mommsenstrasse 13, D 01062 Dresden, Germany
    Microbiology 149:589-600. 2003
    ..Fluorescence microscopy confirmed a localization to the plasma membrane. A model is presented describing a hypothetical function of Gpr1p during adaptation to acetic acid...
  45. ncbi request reprint Subcellular localization of yeast Sec14 homologues and their involvement in regulation of phospholipid turnover
    Martina Schnabl
    Department of Biochemistry, University of Technology, Graz, Austria
    Eur J Biochem 270:3133-45. 2003
    ..In summary, Sfh2p and Sfh4p are the Sec14p homologues with the most pronounced functional similarity to Sec14p, whereas the other Sfh proteins appear to be functionally less related to Sec14p...
  46. pmc Mitochondrial phosphate-carrier deficiency: a novel disorder of oxidative phosphorylation
    Johannes A Mayr
    Department of Pediatrics, Paracelsus Private Medical University, Salzburg, Austria
    Am J Hum Genet 80:478-84. 2007
    ..The enzyme defect was confirmed by complementation analysis in yeast. This is the first report of patients with mitochondrial phosphate-carrier deficiency...
  47. ncbi request reprint The role of respiration, reactive oxygen species and oxidative stress in mother cell-specific ageing of yeast strains defective in the RAS signalling pathway
    Gino Heeren
    Department of Cell Biology, University of Salzburg, Hellbrunnerstrasse 34, A 5020 Salzburg, Austria
    FEMS Yeast Res 5:157-67. 2004
    ..Our results indicate that oxidative stress resulting from a disturbance of redox balance can play a major role in mother cell-specific lifespan determination of yeast cells...
  48. ncbi request reprint Sterol binding assay using surface plasmon fluorescence spectroscopy
    Birgit Wiltschi
    Department of Membrane Biochemistry, Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    Anal Chem 78:547-55. 2006
    ..With the design of our novel in vitro oxysterol binding assay, we have solved the technically challenging difficulty of presenting hydrophobic ligands to hydrophilic proteins in aqueous media...
  49. ncbi request reprint Interaction of Pik1p and Sjl proteins in membrane trafficking
    Peter H Nguyen
    Department of Biological Sciences, St John s University, 8000 Utopia Parkway, Jamaica, NY 11439, USA
    FEMS Yeast Res 5:363-71. 2005
    ....