D Klein

Summary

Affiliation: University of Veterinary Medicine
Country: Austria

Publications

  1. pmc Influence of vector design and host cell on the mechanism of recombination and emergence of mutant subpopulations of replicating retroviral vectors
    Matthias Paar
    Department of Pathobiology, Institute of Virology, University of Veterinary Medicine, Vienna, Austria
    BMC Mol Biol 10:8. 2009
  2. ncbi request reprint Proviral load determination of different feline immunodeficiency virus isolates using real-time polymerase chain reaction: influence of mismatches on quantification
    D Klein
    Institute of Virology, University of Veterinary Sciences, Vienna, Austria
    Electrophoresis 20:291-9. 1999
  3. ncbi request reprint Strike against cancer
    Dieter Klein
    Trends Mol Med 8:7. 2002
  4. ncbi request reprint Quantification using real-time PCR technology: applications and limitations
    Dieter Klein
    Institute of Virology, University of Veterinary Medicine, Veterinarplatz 1, A 1210 Vienna, Austria
    Trends Mol Med 8:257-60. 2002
  5. doi request reprint Vascular endothelial (VEGF) and epithelial growth factor (EGF) as well as platelet-activating factor (PAF) and receptors are expressed in the early pregnant canine uterus
    S Schäfer-Somi
    Centre for Artificial Insemination and Embryo Transfer, University of Veterinary Medicine Vienna, Vienna, Austria
    Reprod Domest Anim 48:20-6. 2013
  6. doi request reprint Is apoptosis a regulatory mechanism during early canine pregnancy?
    S Schäfer-Somi
    Centre for Artificial Insemination and Embryo Transfer, University of Veterinary Medicine Vienna, Vienna, Austria
    Reprod Domest Anim 47:169-72. 2012
  7. ncbi request reprint Influence of preassay and sequence variations on viral load determination by a multiplex real-time reverse transcriptase-polymerase chain reaction for feline immunodeficiency virus
    D Klein
    Institute of Virology, University of Veterinary Sciences, Vienna, Austria
    J Acquir Immune Defic Syndr 26:8-20. 2001
  8. doi request reprint Expression of MHC-I and -II in uterine tissue from early pregnant bitches
    S Schäfer-Somi
    Centre for Artificial Insemination and Embryo Transfer, University of Veterinary Science, Vienna, Austria
    Reprod Domest Anim 44:103-8. 2009
  9. ncbi request reprint Composition of parental mitochondrial DNA in cloned bovine embryos
    R Steinborn
    Department of Animal Biotechnology at IFA, Tulln, Austria
    FEBS Lett 426:352-6. 1998
  10. ncbi request reprint Effects of semen extender and semen processing on motility and viability of frozen-thawed dog spermatozoa
    Sabine Schäfer-Somi
    Centre for Artificial Insemination and Embryo Transfer, University of Veterinary Medicine, Vienna 1210, Austria
    Theriogenology 66:173-82. 2006

Collaborators

Detail Information

Publications23

  1. pmc Influence of vector design and host cell on the mechanism of recombination and emergence of mutant subpopulations of replicating retroviral vectors
    Matthias Paar
    Department of Pathobiology, Institute of Virology, University of Veterinary Medicine, Vienna, Austria
    BMC Mol Biol 10:8. 2009
    ..This necessitates efforts to analyse the rate and mechanism of recombinant emergence during the replication of such vectors to provide data which should contribute to improvements in RCR vector design...
  2. ncbi request reprint Proviral load determination of different feline immunodeficiency virus isolates using real-time polymerase chain reaction: influence of mismatches on quantification
    D Klein
    Institute of Virology, University of Veterinary Sciences, Vienna, Austria
    Electrophoresis 20:291-9. 1999
    ..Based on these results, it will be possible to design real-time PCR systems allowing the quantification of a broad range of isolates, which is a prerequisite for the investigation of the impact of viral load in tumorigenesis...
  3. ncbi request reprint Strike against cancer
    Dieter Klein
    Trends Mol Med 8:7. 2002
  4. ncbi request reprint Quantification using real-time PCR technology: applications and limitations
    Dieter Klein
    Institute of Virology, University of Veterinary Medicine, Veterinarplatz 1, A 1210 Vienna, Austria
    Trends Mol Med 8:257-60. 2002
    ..However, the wide use of real-time PCR methods has also highlighted some of the critical points and limitations of these assays. These aspects must be considered to increase the reliability of the obtained data...
  5. doi request reprint Vascular endothelial (VEGF) and epithelial growth factor (EGF) as well as platelet-activating factor (PAF) and receptors are expressed in the early pregnant canine uterus
    S Schäfer-Somi
    Centre for Artificial Insemination and Embryo Transfer, University of Veterinary Medicine Vienna, Vienna, Austria
    Reprod Domest Anim 48:20-6. 2013
    ..The course of EGF was in diametrical opposition to the course of the receptor. These results point towards an increased demand for VEGF, EGF and PAF during the earliest stages of canine pregnancy...
  6. doi request reprint Is apoptosis a regulatory mechanism during early canine pregnancy?
    S Schäfer-Somi
    Centre for Artificial Insemination and Embryo Transfer, University of Veterinary Medicine Vienna, Vienna, Austria
    Reprod Domest Anim 47:169-72. 2012
    ..These preliminary results point towards a regulatory function of the Fas/FasL system during early canine pregnancy...
  7. ncbi request reprint Influence of preassay and sequence variations on viral load determination by a multiplex real-time reverse transcriptase-polymerase chain reaction for feline immunodeficiency virus
    D Klein
    Institute of Virology, University of Veterinary Sciences, Vienna, Austria
    J Acquir Immune Defic Syndr 26:8-20. 2001
    ..In view of the inherent variability of retroviral sequences, these results underline the necessity to check reaction efficiencies before determining viral load...
  8. doi request reprint Expression of MHC-I and -II in uterine tissue from early pregnant bitches
    S Schäfer-Somi
    Centre for Artificial Insemination and Embryo Transfer, University of Veterinary Science, Vienna, Austria
    Reprod Domest Anim 44:103-8. 2009
    ..05). It is proposed that the increase in MHC-II is pregnancy-related, even though the impact on maintenance of canine pregnancy is still unclear...
  9. ncbi request reprint Composition of parental mitochondrial DNA in cloned bovine embryos
    R Steinborn
    Department of Animal Biotechnology at IFA, Tulln, Austria
    FEBS Lett 426:352-6. 1998
    ..The ratio of parental mtDNA was in accordance with the estimated quantitative participation of mtDNA from the fusion partners...
  10. ncbi request reprint Effects of semen extender and semen processing on motility and viability of frozen-thawed dog spermatozoa
    Sabine Schäfer-Somi
    Centre for Artificial Insemination and Embryo Transfer, University of Veterinary Medicine, Vienna 1210, Austria
    Theriogenology 66:173-82. 2006
    ..This was shown to be partly caused by less pronounced fluctuations of temperature inside the straws when compared to box-freezing...
  11. ncbi request reprint Increased interleukin-1beta mRNA expression in skin biopsies of horses with Culicoides hypersensitivity following challenge with Culicoides nubeculosus extract
    Gabriela Kolm
    Department for Animal Breeding and Reproduction, Veterinary University Vienna, Veterinaerplatz 1, A 1210 Vienna, Austria
    Vet Immunol Immunopathol 113:90-8. 2006
    ..C. nubeculosus stimulates local IL-1beta transcription in all horses independent from disease, but the extent of upregulation from basal levels only reaches statistical significance in horses affected by CHS and active stage of disease...
  12. ncbi request reprint Lactoferrin expression in the horse endometrium: relevance in persisting mating-induced endometritis
    Gabriela Kolm
    Department for Animal Breeding and Reproduction, Veterinary University Vienna, Veterinaerplatz 1, A 1210 Vienna, Austria
    Vet Immunol Immunopathol 114:159-67. 2006
    ..Enhanced LF transcription within the endometrial epithelium might therefore be a response to recurrent persisting inflammation following insemination in mares with delayed uterine clearance...
  13. ncbi request reprint Lactoferrin, a glycoprotein with immunomodulatory and mast cell stabilising properties, in skin of horses suffering from Culicoides hypersensitivity
    Gabriela Kolm
    Department for Animal Breeding and Reproduction, University of Veterinary Medicine Vienna, Veterinaerplatz 1, A 1210 Vienna, Austria
    Res Vet Sci 83:165-70. 2007
    ..In contrast to human skin allergen challenged sites, LF mRNA levels in horses affected by CHS are not significantly different to that of control sites at 4h post-injection of C. nubeculosus extract...
  14. ncbi request reprint Detection and quantification of the iap gene of Listeria monocytogenes and Listeria innocua by a new real-time quantitative PCR assay
    I Hein
    Institute of Milk Hygiene, Milk Technology and Food Science, University of Veterinary Medicine, Vienna, Austria
    Res Microbiol 152:37-46. 2001
    ....
  15. ncbi request reprint The murine whey acidic protein promoter directs expression to human mammary tumors after retroviral transduction
    Feride Oztürk-Winder
    Institute of Virology, University of Veterinary Sciences, A 1210 Vienna, Austria
    Cancer Gene Ther 9:421-31. 2002
    ....
  16. pmc Multiple modifications allow high-titer production of retroviral vectors carrying heterologous regulatory elements
    Juraj Hlavaty
    Institute of Virology, University of Veterinary Medicine, A 1210 Vienna, Austria
    J Virol 78:1384-92. 2004
    ..The combination of all three modifications resulted in a 2- to 3-log increase in the virus titer. These modifications result in expression targeted ProCon vectors with titers similar to those of nonmodified MLV-based vectors...
  17. ncbi request reprint Genetic reshuffling reconstitutes functional expression cassettes in retroviral vectors
    W Tabotta
    Institute of Virology, University of Veterinary Sciences, Vienna, Austria
    J Gene Med 3:418-26. 2001
    ....
  18. doi request reprint Uterine progesterone receptor and leukaemia inhibitory factor mRNA expression in canine pregnancy
    S Schäfer-Somi
    Centre for Artificial Insemination and Embryo Transfer, University of Veterinary Medicine, Vienna, Austria
    Reprod Domest Anim 44:109-14. 2009
    ..We additionally propose that canine embryo secretory proteins have a regulatory effect on both PR and LIF before as well as at and after implantation...
  19. ncbi request reprint Detection of bovine torovirus in neonatal calf diarrhoea in Lower Austria and Styria (Austria)
    B Haschek
    Clinic for Ruminants, Department for Farm Animals and Herd Management, University of Veterinary Medicine, Veterinaerplatz 1, A 1210 Vienna, Austria
    J Vet Med B Infect Dis Vet Public Health 53:160-5. 2006
    ..7%; Escherichia coli, 17%; Cryptosporidium spp., 11.7%; Eimeria spp., 10.4%; Rotavirus, 9.1%; Clostridium perfringens, 9.1% and Giardia spp., 6.1%. Salmonella spp. was not detected...
  20. ncbi request reprint Mutations in the catalytic core or the C-terminus of murine leukemia virus (MLV) integrase disrupt virion infectivity and exert diverse effects on reverse transcription
    Adolf Steinrigl
    Research Institute for Virology and Biomedicine, University of Veterinary Medicine, A 1210 Vienna, Austria
    Virology 362:50-9. 2007
    ..Deletion of either the IN C-terminus alone, or in addition to part of the catalytic domain exerted contrasting effects on intracellular viral DNA levels, implying that IN influences reverse transcription in more than one direction...
  21. ncbi request reprint Phylogenetic analysis of feline immunodeficiency virus in Central Europe: a prerequisite for vaccination and molecular diagnostics
    Adolf Steinrigl
    Institute of Virology, University of Veterinary Medicine, Veterinarplatz 1, A 1210 Vienna, Austria
    J Gen Virol 84:1301-7. 2003
    ..The highly coincident results point to the existence of extensive virus variation with the presence of at least two highly divergent subtypes of FIV in Austria and Germany...
  22. doi request reprint Phylogenetic analysis suggests independent introduction of feline immunodeficiency virus clades A and B to Central Europe and identifies diverse variants of clade B
    Adolf Steinrigl
    Institute of Virology, Department of Pathobiology, University of Veterinary Medicine, A 1210 Vienna, Austria
    Vet Immunol Immunopathol 134:82-9. 2010
    ....
  23. ncbi request reprint Genetic variability of PRRS virus in Austria: consequences for molecular diagnostics and viral quantification
    Stanislav Indik
    Research Institute of Virology and Biomedicine, University of Veterinary Medicine, Veterinarplatz 1, A 1210 Vienna, Austria
    Vet Microbiol 107:171-8. 2005
    ..The study highlights the importance of a detailed study on the sequence variations occurring in the region of interest before the development of a reliable PCR-based assay...