Alois Jungbauer

Summary

Affiliation: University of Natural Resources and Applied Life Sciences
Country: Austria

Publications

  1. ncbi Current status of technical protein refolding
    Alois Jungbauer
    Department of Biotechnology, Austrian Center of Biopharmaceutical Technology, University of Natural Resources and Applied Life Sciences Vienna, Muthgasse 18, A 1190 Vienna, Austria
    J Biotechnol 128:587-96. 2007
  2. doi Phytoestrogens and the metabolic syndrome
    Alois Jungbauer
    Christian Doppler Laboratory of Receptor Biotechnology, Department of Biotechnology, University of Natural Resources and Life Sciences Vienna, Vienna, Austria Electronic address
    J Steroid Biochem Mol Biol 139:277-89. 2014
  3. doi Continuous downstream processing of biopharmaceuticals
    Alois Jungbauer
    Department of Biotechnology, University of Natural Resources and Life Sciences Vienna BOKU, Vienna, Austria
    Trends Biotechnol 31:479-92. 2013
  4. doi Editorial: Biopharmaceuticals - discovery, development and manufacturing
    Alois Jungbauer
    University of Natural Resources and Life Sciences, Vienna, Austria
    Biotechnol J 7:1422-3. 2012
  5. doi Editorial: Biotechnology's impact on sustainable development
    Alois Jungbauer
    University of Natural Resources and Life Sciences, Vienna
    Biotechnol J 7:1317. 2012
  6. doi Anti-inflammatory properties of culinary herbs and spices that ameliorate the effects of metabolic syndrome
    Alois Jungbauer
    Department of Biotechnology and Christian Doppler Laboratory of Receptor Biotechnology, University of Natural Resources and Life Sciences Vienna, Muthgasse 18, 1190 Vienna, Austria
    Maturitas 71:227-39. 2012
  7. ncbi Hydrophobic interaction chromatography of proteins. III. Unfolding of proteins upon adsorption
    Alois Jungbauer
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
    J Chromatogr A 1079:221-8. 2005
  8. ncbi Monoliths for fast bioseparation and bioconversion and their applications in biotechnology
    Alois Jungbauer
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria
    J Sep Sci 27:767-78. 2004
  9. ncbi Engineering protein A affinity chromatography
    Alois Jungbauer
    University of Natural Resources and Applied Life Sciences and Austrian Center of Biopharmaceutical Technology, Department of Biotechnology, Muthgasse 18, 1190 Vienna, Austria
    Curr Opin Drug Discov Devel 7:248-56. 2004
  10. doi Polymethacrylate monoliths for preparative and industrial separation of biomolecular assemblies
    Alois Jungbauer
    Austrian Center of Biopharmaceutical Technology, University of Natural Resources and Applied Life Sciences Vienna, Vienna, Austria
    J Chromatogr A 1184:62-79. 2008

Collaborators

Detail Information

Publications90

  1. ncbi Current status of technical protein refolding
    Alois Jungbauer
    Department of Biotechnology, Austrian Center of Biopharmaceutical Technology, University of Natural Resources and Applied Life Sciences Vienna, Muthgasse 18, A 1190 Vienna, Austria
    J Biotechnol 128:587-96. 2007
    ..The major refolding operations such as dilution, matrix assisted refolding, pressure driven refolding or continuous refolding applications are discussed in view of industrial applicability...
  2. doi Phytoestrogens and the metabolic syndrome
    Alois Jungbauer
    Christian Doppler Laboratory of Receptor Biotechnology, Department of Biotechnology, University of Natural Resources and Life Sciences Vienna, Vienna, Austria Electronic address
    J Steroid Biochem Mol Biol 139:277-89. 2014
    ..Nevertheless, extracts rich in phytoestrogens may be an alternative treatment or may complement conventional treatment for diseases linked with metabolic syndrome. This article is part of a Special Issue entitled 'Phytoestrogens'. ..
  3. doi Continuous downstream processing of biopharmaceuticals
    Alois Jungbauer
    Department of Biotechnology, University of Natural Resources and Life Sciences Vienna BOKU, Vienna, Austria
    Trends Biotechnol 31:479-92. 2013
    ..Economic pressure, flexibility, and parametric release considerations will be the driving force to implement continuous manufacturing strategies in future. ..
  4. doi Editorial: Biopharmaceuticals - discovery, development and manufacturing
    Alois Jungbauer
    University of Natural Resources and Life Sciences, Vienna, Austria
    Biotechnol J 7:1422-3. 2012
    This Special issue, Biopharmaceuticals - discovery, development and manufacturing is edited by Alois Jungbauer and Klaus Graumann and includes articles on various aspects of the development of biopharmaceuticals...
  5. doi Editorial: Biotechnology's impact on sustainable development
    Alois Jungbauer
    University of Natural Resources and Life Sciences, Vienna
    Biotechnol J 7:1317. 2012
    ..It is clear that bioenergy and biofuel cannot solve a world energy crisis or reverse global warming, but from a local perspective it can contribute a lot...
  6. doi Anti-inflammatory properties of culinary herbs and spices that ameliorate the effects of metabolic syndrome
    Alois Jungbauer
    Department of Biotechnology and Christian Doppler Laboratory of Receptor Biotechnology, University of Natural Resources and Life Sciences Vienna, Muthgasse 18, 1190 Vienna, Austria
    Maturitas 71:227-39. 2012
    ..The effects of chronic inflammation caused by obesity are counteracted and, consequently, the progression of diseases associated with chronic inflammation slowed...
  7. ncbi Hydrophobic interaction chromatography of proteins. III. Unfolding of proteins upon adsorption
    Alois Jungbauer
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
    J Chromatogr A 1079:221-8. 2005
    ..Furthermore, we hypothesize that the surface acts as catalyst for partial unfolding, since the fraction of partial unfolded protein is increasing with length of the alkyl chain...
  8. ncbi Monoliths for fast bioseparation and bioconversion and their applications in biotechnology
    Alois Jungbauer
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria
    J Sep Sci 27:767-78. 2004
    ..In addition, monoliths will further substantially advance bioseparations of both small and large molecules in the future...
  9. ncbi Engineering protein A affinity chromatography
    Alois Jungbauer
    University of Natural Resources and Applied Life Sciences and Austrian Center of Biopharmaceutical Technology, Department of Biotechnology, Muthgasse 18, 1190 Vienna, Austria
    Curr Opin Drug Discov Devel 7:248-56. 2004
    ..Questions of scale-up, design of chromatographic conditions, clearance of adventitious agents and operational modes, such as continuous operation or purification of antibodies in expanded-bed mode, will be addressed in this review...
  10. doi Polymethacrylate monoliths for preparative and industrial separation of biomolecular assemblies
    Alois Jungbauer
    Austrian Center of Biopharmaceutical Technology, University of Natural Resources and Applied Life Sciences Vienna, Vienna, Austria
    J Chromatogr A 1184:62-79. 2008
    ..This review discusses the pressure drop characteristics, mass transfer properties, scale-up, and applications of monoliths in the context of conventional chromatography media...
  11. ncbi Chromatographic media for bioseparation
    Alois Jungbauer
    Department of Biotechnology, University of Natural Resources and Applied Life Science, A 1190 Vienna, Austria
    J Chromatogr A 1065:3-12. 2005
    ..Common to all modern chromatography media is the fast operation. There are examples where a residence time of less then 3 min, is sufficient to reach the full potential of the adsorbent...
  12. ncbi Folding and refolding of proteins in chromatographic beds
    Alois Jungbauer
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, 1190 Vienna, Austria
    Curr Opin Biotechnol 15:487-94. 2004
    ..Advances have also been made in the use of immobilized metal affinity chromatography and by mimicking the natural folding process with artificial chaperones...
  13. ncbi Performance and characterization of a nanophased porous hydroxyapatite for protein chromatography
    Alois Jungbauer
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria
    Biotechnol Bioeng 87:364-75. 2004
    ..This nanophased material comprises a novel stationary phase for IgG separation...
  14. doi Two-dimensional difference fluorescence gel electrophoresis to verify the scale-up of a non-affinity-based downstream process for isolation of a therapeutic recombinant antibody
    Julita K Grzeskowiak
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences Vienna, Vienna, Austria
    Electrophoresis 31:1862-72. 2010
    ..2-D DIGE is a helpful tool for monitoring of purification effects and for scale-up verification of downstream processes...
  15. doi Hydrophobic interaction chromatography of proteins: thermodynamic analysis of conformational changes
    Rene Ueberbacher
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences Vienna, Muthgasse 18, Vienna, Austria
    J Chromatogr A 1217:184-90. 2010
    ..Discrepancies between DeltaH(ads) determined by van't Hoff analysis and ITC were observed. We explain this with protein conformational changes upon adsorption which are not accounted for by van't Hoff analysis...
  16. doi Refolding of Npro fusion proteins
    Waltraud Kaar
    Austrian Center of Biopharmaceutical Technology, Vienna, Austria
    Biotechnol Bioeng 104:774-84. 2009
    ..At high protein concentrations, deviations from the model assumptions were observed and thus smaller rate constants were required to approximate the data...
  17. doi Matrix-assisted refolding of autoprotease fusion proteins on an ion exchange column
    Elisabeth Schmoeger
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences Vienna, Muthgasse 18, 1190 Vienna, Austria
    J Chromatogr A 1216:8460-9. 2009
    ..This chromatographic refolding process allows processing of fusion peptides at a concentration range 10- to 100-fold higher than that observed for common refolding systems...
  18. ncbi Mapping of FVIII inhibitor epitopes using cellulose-bound synthetic peptide arrays
    Eva Maria Kopecky
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
    J Immunol Methods 308:90-100. 2006
    ..Here, we demonstrate that the SPOT method is very well suited for the precise location of epitopes in the core of the protein, which usually cannot be detected by other methods...
  19. ncbi 2-D DIGE to expedite downstream process development for human monoclonal antibody purification
    Julita K Grzeskowiak
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences Vienna, Muthgasse 18, Vienna 1190, Austria
    Protein Expr Purif 66:58-65. 2009
    ..After purification of the IgG from different culture supernatants, the protein patterns on 2-D DIGE were identical, indicating the validity of purification scheme...
  20. doi Peptide affinity chromatography media that bind N(pro) fusion proteins under chaotropic conditions
    Rainer Hahn
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences Vienna, Muthgasse 18, 1190 Vienna, Austria
    J Chromatogr A 1217:6203-13. 2010
    ..Binding capacities were in the range of 7-15 mg protein mL(-1) of medium, as could be shown for several EDDIE fusion proteins. An efficient protocol for autoproteolytic cleavage using an on-column refolding method was implemented...
  21. doi Receptor binding and transactivation activities of red clover isoflavones and their metabolites
    Angelika Pfitscher
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria
    J Steroid Biochem Mol Biol 112:87-94. 2008
    ..We propose that other metabolites can also contribute to these effects. However, more detailed information for the formation of these metabolites in humans and for bioavailability data are required to confirm our assumptions...
  22. doi Autoprotease N(pro): analysis of self-cleaving fusion protein
    Martin Wellhoefer
    Austrian Centre of Industrial Biotechnology ACIB, Muthgasse 11, 1190 Vienna, Austria
    J Chromatogr A 1304:92-100. 2013
    ..It was shown that the initial EDDIE-pep6His concentration in the refolding solution decreased from 194.3 to 83.8μg/ml over a refolding time of 385min resulting in a final refolding and cleavage yield of 50%. ..
  23. ncbi Dispersion effects in preparative polymethacrylate monoliths operated in radial-flow columns
    Rainer Hahn
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences Vienna, Muthgasse 18, 1190 Vienna, Austria
    J Biochem Biophys Methods 70:87-94. 2007
    ....
  24. doi Adsorption of plasmid DNA on ceramic hydroxyapatite chromatographic materials
    Elisabeth Schmoeger
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences Vienna, Vienna, Austria
    J Sep Sci 33:3125-36. 2010
    ..The maximal binding capacity shows that at least a part of the CHT II bead must be accessible for the plasmid, whereas CHT III can be saturated with the plasmid. In both cases, an extremely hindered transport takes place...
  25. doi Matrix-assisted refolding of autoprotease fusion proteins on an ion exchange column: a kinetic investigation
    Elisabeth Schmoeger
    Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Muthgasse 18, 1190 Vienna, Austria
    J Chromatogr A 1217:5950-6. 2010
    ..Processing the autoprotease fusion protein on Capto S at a high protein loading of 50 mg mL(-1) gel and short contact time (0.5h) yielded the highest productivity...
  26. doi Surface energies of hydrophobic interaction chromatography media by inverse liquid chromatography
    Ingeborg Bednar
    Austrian Centre of Industrial Biotechnology, Muthgasse 11, 1190 Vienna, Austria
    J Chromatogr A 1220:115-21. 2012
    ....
  27. ncbi Identification of a ligand for IgG-Fc derived from a soluble peptide library based on fusion proteins secreted by S. cerevisiae
    Christa Mersich
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences Vienna, Vienna, Austria
    Biotechnol J 2:672-7. 2007
    ..An affinity constant of 3.9 x 10(5) M(-1) was determined by a biosensor method. Handling and maintenance of this library is conceptually simple and highly applicable for automated high-throughput systems...
  28. doi Two-dimensional fluorescence difference gel electrophoresis for comparison of affinity and non-affinity based downstream processing of recombinant monoclonal antibody
    Julita K Grzeskowiak
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Muthgasse 18, A 1190 Vienna, Austria
    J Chromatogr A 1216:4902-12. 2009
    ..Thus, 2-D DIGE is a valuable tool for downstream process development...
  29. ncbi Effect of oriented or random PEGylation on bioactivity of a factor VIII inhibitor blocking peptide
    Eva Maria Kopecky
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences Vienna, Vienna, Austria
    Biotechnol Bioeng 93:647-55. 2006
    ..The peptide lost its neutralization properties when PEG was coupled to the N-terminus, again indicating that this part of the peptide is important for functionality...
  30. ncbi Directed immobilization of peptide ligands to accessible pore sites by conjugation with a placeholder molecule
    Rainer Hahn
    Institute for Applied Microbiology, University of Agricultural Sciences, A 1190 Vienna, Austria
    Anal Chem 75:543-8. 2003
    ..This novel immobilization technique substantially increased the binding capacity and the ligand utilization for the target protein, and site-specific immobilization could be demonstrated...
  31. doi Red clover extract: a source for substances that activate peroxisome proliferator-activated receptor alpha and ameliorate the cytokine secretion profile of lipopolysaccharide-stimulated macrophages
    Monika Mueller
    Christian Doppler Laboratory for Receptor Biotechnology, Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Vienna, Austria
    Menopause 17:379-87. 2010
    ..In this study, we determined the putative anti-inflammatory and PPARalpha stimulatory activities of red clover, an alternative to the classic hormone therapy used currently to treat menopausal symptoms...
  32. doi Adsorption of plasmid DNA on anion exchange chromatography media
    Christina Tarmann
    Department of Biotechnology, University of Applied Life Science and Natural Resources Vienna, Vienna, Austria
    J Sep Sci 31:2605-18. 2008
    ..Feed concentrations higher than 0.1 mg/mL pDNA pronounce this effect. Monolithic media showed the fastest adsorption rate and highest binding capacity with 13 mg pDNA per mL...
  33. doi Atomistic structure of monomolecular surface layer self-assemblies: toward functionalized nanostructures
    Christine Horejs
    Department for NanoBiotechnology, University of Natural Resources and Life Sciences, Vienna, Austria
    ACS Nano 5:2288-97. 2011
    ..To the best of our knowledge, this is the first structural model at an amino acid level of an S-layer unit cell that exhibits p4 lattice symmetry...
  34. ncbi Hydrophobic interaction chromatography of proteins IV. Kinetics of protein spreading
    Emmerich Haimer
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
    J Chromatogr A 1139:84-94. 2007
    ..Our findings suggest that proteins which are sensitive to conformational changes should be loaded at high salt concentrations in order to accelerate the adsorption reaction and to obtain steeper breakthrough curves...
  35. doi High-throughput system for determining dissolution kinetics of inclusion bodies
    Astrid Dürauer
    Austrian Center of Biopharmaceutical Technology, Department of Biotechnology, University of Natural Resources and Applied Life Sciences Vienna, Austria
    Biotechnol J 4:722-9. 2009
    ....
  36. doi Comparison of hormonal activity of isoflavone-containing supplements used to treat menopausal complaints
    Evelyne Reiter
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria
    Menopause 16:1049-60. 2009
    ..These preparations contain varying amounts of active ingredients, often without detailed specifications. Thus, it is difficult to derive a recommended daily dose, and the reliability of these products is rather low...
  37. doi PPARα activation by culinary herbs and spices
    Monika Mueller
    Christian Doppler Laboratory for Receptor Biotechnology, Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria
    Planta Med 77:497-504. 2011
    ..Resveratrol and apigenin only slightly transactivated PPARα. These results suggest that a diet rich in fruit, herbs, and spices provides a number of PPARα agonists that might contribute to an improved lipid profile...
  38. doi An immunoaffinity column with a monoclonal antibody as ligand for human follicle stimulating hormone
    Marlene Zandian
    Department of Biotechnology, University of Applied Life Sciences and Natural Resources, Vienna, Austria
    J Sep Sci 32:1585-91. 2009
    ..A great stability of the matrix was confirmed by performing 100 consecutive chromatographic runs without loss of capacity...
  39. ncbi Peptides derived from a secretory yeast library restore factor VIII activity in the presence of an inhibitory antibody
    Christa Mersich
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences Vienna, Vienna, Austria
    Biotechnol Bioeng 98:12-21. 2007
    ..This study demonstrates the potential of this system to identify peptides that inhibit the activity of potent inhibitory antibodies and also shows potential as a method for screening of bioactive peptides...
  40. doi Hydrophobic interaction chromatography of proteins V. Quantitative assessment of conformational changes
    Rene Ueberbacher
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences Vienna, Vienna, Austria
    J Chromatogr A 1198:154-63. 2008
    ..Lower temperatures and binding capacities significantly reduced protein unfolding; the activation energy for unfolding ranged from 47 to 125 kJ/mol...
  41. ncbi Thermodynamic stability and formation of aggregates of human immunoglobulin G characterised by differential scanning calorimetry and dynamic light scattering
    Karin Ahrer
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
    J Biochem Biophys Methods 66:73-86. 2006
    ....
  42. doi Engineering properties of a camelid antibody affinity sorbent for Immunoglobulin G purification
    Marlene Zandian
    Department of Biotechnology, University of Applied Life Sciences and Natural Resources Vienna, Muthgasse 18, A 1190 Vienna, Austria
    J Chromatogr A 1216:5548-56. 2009
    ..Determined engineering parameters were used to predict breakthrough behaviour in column mode considering film and pore resistances. The dynamic binding capacity at 10% breakthrough did not change when residence time was at least 6 min...
  43. doi Red clover extract: a putative source for simultaneous treatment of menopausal disorders and the metabolic syndrome
    Monika Mueller
    Christian Doppler Laboratory for Receptor Biotechnology, Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria
    Menopause 15:1120-31. 2008
    ..This study was designed to determine the peroxisome proliferator-activated receptor (PPAR) gamma activation by red clover extract...
  44. doi Yeast cell surface display system for determination of humoral response to active immunization with a monoclonal antibody against EpCAM
    Astrid Dürauer
    Department of Biotechnology, Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences Vienna, Muthgasse 18, 1190 Vienna, Austria
    J Biochem Biophys Methods 70:1109-15. 2008
    ..Though using this completely different method, experiments with pre-immune and immune sera of four monkeys exemplarily are comparable to the results obtained by analysis with synthetic peptide arrays...
  45. doi Technical refolding of proteins: Do we have freedom to operate?
    Maria K Eiberle
    Boehringer Ingelheim RCV and Co GmbH, Vienna, Austria
    Biotechnol J 5:547-59. 2010
    ..Concepts of continuous refolding have been introduced to reduce tank sizes and increase yields. Some of the patents covering refolding of proteins will soon expire or have already expired. This gives more freedom to operate...
  46. ncbi Hydrophobic interaction chromatography of proteins. II. Binding capacity, recovery and mass transfer properties
    Rainer Hahn
    Institute for Applied Microbiology, University of Agricultural Sciences, Muthgasse 18 A 1190, Vienna, Austria
    J Chromatogr B Analyt Technol Biomed Life Sci 790:99-114. 2003
    ..These sorbents exhibited a disproportional increase of binding capacity with increasing ammonium sulfate concentration. Recovery of proteins above 75% could be observed for all sorbents. Several sorbents showed a recovery close to 100%...
  47. ncbi Continuous matrix-assisted refolding of proteins
    Robert Schlegl
    Boehringer Ingelheim Austria GmbH, Vienna, Austria
    J Chromatogr A 1009:119-32. 2003
    ..The method can be also applied to other chromatographic principles suited for the separation of aggregates...
  48. ncbi N(pro) fusion technology to produce proteins with authentic N termini in E. coli
    Clemens Achmüller
    Austrian Center of Biopharmaceutical Technology, Muthgasse 18, A 1190 Vienna, Austria
    Nat Methods 4:1037-43. 2007
    ..This N(pro) expression system can be used as a generic tool for the high-level production of recombinant toxic peptides and proteins (up to 12 g/l) in Escherichia coli without the need for chemical or enzymatic removal of the fusion tag...
  49. ncbi Continuous matrix assisted refolding of alpha-lactalbumin by ion exchange chromatography with recycling of aggregates combined with ultradiafiltration
    Christine Machold
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
    J Chromatogr A 1080:29-42. 2005
    ..The operability of the system was demonstrated but limitations due to instability of the P-CAC, especially inhomogeneous flow and peak wobbling, have to be considered...
  50. doi Red clover and soy isoflavones--an in vitro safety assessment
    Evelyne Reiter
    Department of Biotechnology, University of Natural Resources and Life Sciences Vienna, Vienna, Austria
    Gynecol Endocrinol 27:1037-42. 2011
    ..These results indicate that isoflavones can be considered safe compounds...
  51. ncbi Combinatorial peptides directed to inhibitory antibodies against human blood clotting factor VIII
    Eva Maria Kopecky
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria
    Thromb Haemost 94:933-41. 2005
    ..Combinatorial peptides could be novel and highly effective drug candidates for alternative treatment in patients with factor VIII inhibitors...
  52. pmc Potential health-modulating effects of isoflavones and metabolites via activation of PPAR and AhR
    Svjetlana Medjakovic
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences Vienna, Vienna, Austria
    Nutrients 2:241-79. 2010
    ..This review discusses effects mediated by the activation of AhR and PPARs and casts a light on the concerted action of isoflavones...
  53. ncbi Androgen receptor transactivation assay using green fluorescent protein as a reporter
    Verena Beck
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, 1190 Vienna, Austria
    Anal Biochem 373:263-71. 2008
    ..The newly developed androgen receptor transactivation assay is a useful tool for characterizing compounds with androgenic activity...
  54. ncbi Mass transfer characteristics of plasmids in monoliths
    Alfred Zöchling
    Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
    J Sep Sci 27:819-27. 2004
    ..These types of monoliths are considered to be the preferred adsorbents for plasmid separation...
  55. doi Isoflavones are safe compounds for therapeutical applications - evaluation of in vitro data
    Evelyne Reiter
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences Vienna, Muthgasse 18, Vienna, Austria
    Gynecol Endocrinol 25:554-80. 2009
    ..In light of the in vitro experiments, together with the epidemiological observations and the clinical experience, isoflavones can be considered as safe compounds and their consumption as food and food supplements has to be promoted...
  56. ncbi Kinetic analysis of estrogen receptor homo- and heterodimerization in vitro
    Elisabeth Jisa
    Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
    J Steroid Biochem Mol Biol 84:141-8. 2003
    ..2 x 10(-8) to 5.4 x 10(-8) and 1.8 x 10(-8) to 2.6 x 10(-8)M for the heterodimer formation. The homo- and heterodimer formation was characterized by a slow association kinetics and kinetic rate constants were within the same range...
  57. ncbi Hydrophobic interaction chromatography of proteins. I. Comparison of selectivity
    Christine Machold
    Institute for Applied Microbiology, University of Agricultural Sciences, Vienna, Austria
    J Chromatogr A 972:3-19. 2002
    ..A good agreement was achieved between the developed evaluation procedure and the separation behaviour of the real feed stock...
  58. doi Adsorption of pDNA on microparticulate charged surface
    Christina Paril
    Department of Biotechnology, University of Applied Life Science and Natural Resources Vienna, Vienna, Austria
    J Biotechnol 141:47-57. 2009
    ..The implementation of microparticles in a lab-scale capture step out of processed E. coli lysates demonstrated their potential as a high speed and high capacity material for pDNA purification...
  59. ncbi GFP-reporter for a high throughput assay to monitor estrogenic compounds
    Verena Beck
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, 1190 Vienna, Austria
    J Biochem Biophys Methods 64:19-37. 2005
    ..Thus, the newly developed yeast estrogen screen using GFP as a reporter can substitute the assay that has been used for a period of several years...
  60. ncbi Matrix assisted refolding of proteins by ion exchange chromatography
    Christine Machold
    Department of Biotechnology, Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, Vienna 1190, Austria
    J Biotechnol 117:83-97. 2005
    ..Refolding on the column is characterized by a low yield but high productivity due to significant reduction of refolding time...
  61. doi Monolith peptide affinity chromatography for quantification of immunoglobulin M
    Silke Neff
    Department of Biotechnology, University of Natural Resources and Life Sciences Vienna, Vienna, Austria
    J Chromatogr A 1218:2374-80. 2011
    ..The binding capacity was consistent within the period of the study which included more than 200 cycles. The analysis time of less than 2 min is an advantage over existing chromatographic methods that rely on pore diffusion...
  62. ncbi Comparison of protein A affinity sorbents III. Life time study
    Rainer Hahn
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
    J Chromatogr A 1102:224-31. 2006
    ..Host cell protein content of eluates from MabSelect Xtra and SuRe were between 300 and 700 ppm, whereas for ProSep-vA Ultra 3000-4000 ppm was achieved...
  63. doi Prediction of inclusion body solubilization from shaken to stirred reactors
    Cornelia Walther
    Austrian Centre of Industrial Biotechnology, Muthgasse 11, 1190, Vienna, Austria
    Biotechnol Bioeng 111:84-94. 2014
    ..The high-throughput system developed on the µ-scale can predict solubilization in stirred reactors up to a factor of 500 and can therefore be used to determine optimal solubilization conditions on laboratory and industrial scale...
  64. doi Pomegranate: a fruit that ameliorates metabolic syndrome
    Svjetlana Medjakovic
    Department of Biotechnology, Christian Doppler Laboratory of Receptor Biotechnology, University of Natural Resources and Life Sciences Vienna, Muthgasse 18, 1190 Vienna, Austria
    Food Funct 4:19-39. 2013
    ..Although the fruit is consumed in many countries, epidemiological and clinical studies are unavailable. Additional research is necessary to corroborate the promise of current in vivo and in vitro findings...
  65. doi Peroxisome proliferator-activated receptor gamma is constitutively activated in yeast
    Monika Mueller
    Christian Doppler Laboratory for Receptor Biotechnology, Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria
    Anal Biochem 385:365-7. 2009
    ..In yeast we identified oleic acid as a putative endogenous ligand. Furthermore yeasts seem to lack regulatory mechanisms present in mammalian cells. Mammalian systems are an alternative for screening PPARgamma agonists...
  66. ncbi Comparison of protein A affinity sorbents II. Mass transfer properties
    Rainer Hahn
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
    J Chromatogr A 1093:98-110. 2005
    ..The dynamic binding capacity of all three media did not change when residence time was at least 4 min. All three media are suited for capture of feed stocks with high antibody content...
  67. ncbi Chromatographic and electrophoretic characterization of protein variants
    Karin Ahrer
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences and Austrian Center of Biopharmaceutical Technology, Vienna, Muthgasse 18, A 1190 Vienna, Austria
    J Chromatogr B Analyt Technol Biomed Life Sci 841:110-22. 2006
    ..This review provides a comparison of chromatographic and electrophoretic separation methods to analyze and to prepare protein variants...
  68. ncbi In situ determination of adsorption kinetics of proteins in a finite bath
    Anne Tscheliessnig
    Austrian Center of Biopharmaceutical Technology, Muthgasse 18, A 1190 Vienna, Austria
    J Chromatogr A 1069:23-30. 2005
    ..1-3 g/l) on rProtein A Sepharose FF and MabSelect were used as model system. The effective diffusion coefficients were determined using a pore diffusion model. These values were in good agreement for all methods...
  69. ncbi Yeast reporter system for rapid determination of estrogenic activity
    Alois Jungbauer
    Institute for Applied Microbiology, University of Agricultural Sciences, Muthgasse 18 A 1190, Vienna, Austria
    J Chromatogr B Analyt Technol Biomed Life Sci 777:167-78. 2002
    ..The detection limit is 0.1 ng 17-beta estradiol or an equivalent activity per liter, if a sample can be concentrated 1000-fold. The system has been further characterized by selected compounds with known and unknown estrogenic activity...
  70. doi Ion-exchange chromatography
    Alois Jungbauer
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria
    Methods Enzymol 463:349-71. 2009
    ..In this chapter, the guidance will be provided for binding and elution conditions and selection of stationary phases...
  71. ncbi Generic method for quantification of FLAG-tagged fusion proteins by a real time biosensor
    Christa Mersich
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences Vienna, Muthgasse 18, Vienna, Austria
    J Biochem Biophys Methods 70:555-63. 2007
    ..Further benefits include the short assay time of approximately 5 min, the small amount of sample required (35 microl per injection) and the ability to monitor the binding event in real time...
  72. ncbi Generation of bioactive peptides by biological libraries
    Christa Mersich
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences Vienna, Muthgasse 18, Vienna, Austria
    J Chromatogr B Analyt Technol Biomed Life Sci 861:160-70. 2008
    ..Libraries in different hosts such as phage, bacteria, yeast, insect cells, mammalian cells exhibit higher biosynthetic capabilities. The latest library systems are compared and their strengths and limitations are reviewed...
  73. doi Red wine: a source of potent ligands for peroxisome proliferator-activated receptor γ
    Alfred Zoechling
    Christian Doppler Laboratory for Receptor Biotechnology, Muthgasse 18, A 1190, Vienna, Austria
    Food Funct 2:28-38. 2011
    ..The ameliorating effects of red wine on metabolic diseases may be partially explained by the presence of PPARγ ligands...
  74. doi Oregano: a source for peroxisome proliferator-activated receptor gamma antagonists
    Monika Mueller
    Christian Doppler Laboratory for Receptor Biotechnology, Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria
    J Agric Food Chem 56:11621-30. 2008
    ..The results warrant further investigation of oregano extract for its potential to prevent and ameliorate metabolic syndrome and its complications...
  75. ncbi Comparison of protein A affinity sorbents
    Rainer Hahn
    Institute for Applied Microbiology, University of Agricultural Sciences, Muthgasse 18, A 1190, Vienna, Austria
    J Chromatogr B Analyt Technol Biomed Life Sci 790:35-51. 2003
    ..Under the applied conditions several sorbents showed very tight binding of IgG and in some cases most of the sample remained on the sorbent. The study can be useful as a guide for optimization of large-scale purification processes...
  76. ncbi Direct synthesis of peptides on convective interaction media monolithic columns for affinity chromatography
    Karin Pflegerl
    Institute of Applied Microbiology, University of Agricultural Sciences, Muthgasse 18, A 1190 Vienna, Austria
    J Comb Chem 4:33-7. 2002
    ..The functionality of the CIM monolithic supports was proven by affinity chromatography of factor VIII. The ammonia-functionalized support performed with low hydrophobicity and did not show unspecific adsorption of proteins...
  77. ncbi Complex formation of a calcium-dependent antibody: a thermodynamical consideration
    Adelheid Einhauer
    Institute for Applied Microbiology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, 1190 Vienna, Austria
    J Chromatogr A 1009:81-7. 2003
    ..Therefore, complex dissociation with EDTA must be effected during a transition state of complex formation and dissociation...
  78. ncbi Comparison of reversed-phase liquid chromatography and hydrophilic interaction/cation-exchange chromatography for the separation of amphipathic alpha-helical peptides with L- and D-amino acid substitutions in the hydrophilic face
    Eva Hartmann
    Institute of Applied Microbiology, University of Agricultural Sciences, 1190 Vienna, Austria
    J Chromatogr A 1009:61-71. 2003
    ..Our results again highlight the potential of HILIC/CEX as a peptide separation mode in its own right as well as an excellent complement to RP-HPLC...
  79. ncbi Recombinant autofluorescent landmarks for standardization of electrophoretic migration of proteins
    Adelheid Einhauer
    Institute of Applied Microbiology, University of Agricultural Sciences, Vienna, Austria
    Electrophoresis 23:1146-52. 2002
    ..The quality and applicability was tested in 1-D and 2-D electrophoresis. Sharp bands or symmetric spots were obtained. The proteins are considered as a new generation of reference proteins for electrokinetic separation methods...
  80. doi Construction and development of a new single-column simulated moving bed system on the laboratory scale
    Kerstin Buhlert
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences Vienna, Muthgasse 18, A 1190 Vienna, Austria
    J Chromatogr A 1216:8778-86. 2009
    ..Furthermore, the SC-SMB unit is operated with a modified AKTA Explorer workstation, which has been specifically developed for the handling of biological fluids...
  81. ncbi Estrogenic activity in white and red wine extracts
    Carolyn M Klinge
    Department of Biochemistry and Molecular Biology, University of Louisville School of Medicine, Louisville, Kentucky 40292, USA
    J Agric Food Chem 51:1850-7. 2003
    ..Chemical analysis clearly showed that the trans-resveratrol content of the red wine extracts was 1 order of magnitude below the detection limit for YES assay...
  82. ncbi Editorial: biopharmaceutical technologies
    Alois Jungbauer
    Biotechnol J 2:647. 2007
  83. ncbi About the cover: The Z basic protein tag
    Alois Jungbauer
    Biotechnol J 1:113. 2006
  84. ncbi Whey proteins as a model system for chromatographic separation of proteins
    Linda Pedersen
    Engineering Research Centre IVC SEP, Department of Chemical Engineering, Technical University of Denmark, Building 229, DK 2800, Lyngby, Denmark
    J Chromatogr B Analyt Technol Biomed Life Sci 790:161-73. 2003
    ....
  85. doi Waste biotech
    Alois Jungbauer
    Biotechnol J 3:831. 2008
  86. ncbi Application of monoliths for plasmid DNA purification development and transfer to production
    Jochen Urthaler
    Boehringer Ingelheim Austria GmbH, Dr Boehringer Gasse 5 11, A 1121 Vienna, Austria
    J Chromatogr A 1065:93-106. 2005
    ..The analytical results obtained from these runs confirmed suitability for pharmaceutical applications...
  87. doi Editorial: between molecular and macroscopic protein engineering
    Alois Jungbauer
    Biotechnol J 3:143. 2008
  88. ncbi Alois Jungbauer: protein bioengineering
    Alois Jungbauer
    Biotechnol J 1:26-7. 2006
  89. ncbi The art of bioprocessing
    Alois Jungbauer
    Biotechnol J 1:111. 2006
  90. ncbi Retention studies of DNA on anion-exchange monolith chromatography Binding site and elution behavior
    Shuichi Yamamoto
    Department of Chemical Engineering, School of Engineering, Applied Medical Engineering Science Division, Graduate School of Medicine, Yamaguchi University, Tokiwadai, Ube 755 8611, Japan
    J Chromatogr A 1144:155-60. 2007
    ..5M) and a high flow-velocity (2.7 cm/min)...