Antonius C F Gorren

Summary

Affiliation: University of Graz
Country: Austria

Publications

  1. ncbi request reprint Bioactivation of pentaerythrityl tetranitrate by mitochondrial aldehyde dehydrogenase
    Martina Griesberger
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Graz, Austria
    Mol Pharmacol 79:541-8. 2011
  2. ncbi request reprint High-pressure studies of the reaction mechanism of nitric-oxide synthase
    Antonius C F Gorren
    Institut fur Pharmakologie und Toxikologie, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
    Biochim Biophys Acta 1764:578-85. 2006
  3. ncbi request reprint Nitric-oxide synthase: a cytochrome P450 family foster child
    Antonius C F Gorren
    Department of Pharmacology und Toxicology, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
    Biochim Biophys Acta 1770:432-45. 2007
  4. ncbi request reprint Site-directed mutagenesis of aldehyde dehydrogenase-2 suggests three distinct pathways of nitroglycerin biotransformation
    M Verena Wenzl
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010, Austria
    Mol Pharmacol 80:258-66. 2011
  5. pmc Characterization of the East Asian variant of aldehyde dehydrogenase-2: bioactivation of nitroglycerin and effects of Alda-1
    Matteo Beretta
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, 8010 Graz, Austria
    J Biol Chem 285:943-52. 2010
  6. pmc Role of the general base Glu-268 in nitroglycerin bioactivation and superoxide formation by aldehyde dehydrogenase-2
    M Verena Wenzl
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, A 8010 Graz, Austria
    J Biol Chem 284:19878-86. 2009
  7. ncbi request reprint Potent inhibition of aldehyde dehydrogenase-2 by diphenyleneiodonium: focus on nitroglycerin bioactivation
    Regina Neubauer
    Department of Pharmacology and Toxicology, Karl Franzens Universität Graz, Austria
    Mol Pharmacol 84:407-14. 2013
  8. ncbi request reprint Tetrahydrobiopterin protects soluble guanylate cyclase against oxidative inactivation
    Kurt Schmidt
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Graz, Austria
    Mol Pharmacol 82:420-7. 2012
  9. pmc Partially irreversible inactivation of mitochondrial aldehyde dehydrogenase by nitroglycerin
    Matteo Beretta
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, A 8010 Graz, Austria
    J Biol Chem 283:30735-44. 2008
  10. ncbi request reprint Bioactivation of nitroglycerin by ascorbate
    Alexander Kollau
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
    Mol Pharmacol 72:191-6. 2007

Collaborators

  • Bernd Mayer
  • Kurt Schmidt
  • Ernst R Werner
  • Reinhard Lange
  • D Koesling
  • K Kristoffer Andersson
  • Johannes Peter Stasch
  • Robert Weis
  • K Gruber
  • Richard C Venema
  • Claude Balny
  • Dennis Stuehr
  • Daniel Mansuy
  • R Heller
  • Matteo Beretta
  • M Verena Wenzl
  • Michael Russwurm
  • Bernd Kolesnik
  • Stephane Marchal
  • Astrid Schrammel
  • Christian L Heine
  • Martina Griesberger
  • Alexander Kollau
  • Renate Schmidt
  • Regina Neubauer
  • Barbara S Lang
  • Henrik Zakariassen
  • Marc Pignitter
  • Morten Sørlie
  • Toru Shimizu
  • Stéphane Marchal
  • Knut Teigen
  • Rüdiger Wippel
  • Regina Golser
  • Hans Jörg Habisch
  • Morten Sorlie
  • David Lefevre-Groboillot
  • Heike Stessel
  • Andrea Neubauer
  • Gerald Wölkart
  • Barbara Lang
  • Christine Schwarzenegger
  • Knut Palten
  • Cristina M Furdui
  • Leslie B Poole
  • Gustav Oberdorfer
  • Gerald Wölkart
  • Pravas K Baral
  • Andreas Zeller
  • Astrid Sottler
  • Espen Harbitz
  • F Henning Cederkvist
  • Saviana Nedeianu
  • Khanh K Dao
  • Jan Haavik
  • Nils Åge Frøystein
  • Jeffrey A McKinney
  • Morten Sørlie
  • Margit Rehn
  • Aurora Martinez
  • Jean Luc Boucher
  • Alain Desbois
  • Hazel Mary Girvan
  • Jean Luc Zimmermann
  • Silvia Pfeiffer
  • Haiying Liang
  • Yves Frapart
  • John F Parkinson
  • Andrew William Munro

Detail Information

Publications33

  1. ncbi request reprint Bioactivation of pentaerythrityl tetranitrate by mitochondrial aldehyde dehydrogenase
    Martina Griesberger
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Graz, Austria
    Mol Pharmacol 79:541-8. 2011
    ..The very low rate of ALDH2 inactivation, presumably as a result of low affinity of the denitration pathway, may at least partially explain why PETN does not induce vascular tolerance...
  2. ncbi request reprint High-pressure studies of the reaction mechanism of nitric-oxide synthase
    Antonius C F Gorren
    Institut fur Pharmakologie und Toxikologie, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
    Biochim Biophys Acta 1764:578-85. 2006
    ..This exposition is followed by a summary of the results obtained thus far in high-pressure studies and of the conclusions that can be drawn from them...
  3. ncbi request reprint Nitric-oxide synthase: a cytochrome P450 family foster child
    Antonius C F Gorren
    Department of Pharmacology und Toxicology, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
    Biochim Biophys Acta 1770:432-45. 2007
    ..In this review similarities and differences between NOS and cytochrome P450 are analyzed in an attempt to explain why NOS requires BH4 and why NO synthesis is not catalyzed by a member of the cytochrome P450 family...
  4. ncbi request reprint Site-directed mutagenesis of aldehyde dehydrogenase-2 suggests three distinct pathways of nitroglycerin biotransformation
    M Verena Wenzl
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010, Austria
    Mol Pharmacol 80:258-66. 2011
    ..Although the latter reaction accounts for less than 10% of total turnover of GTN metabolism catalyzed by wild-type ALDH2, it is most likely essential for vascular GTN bioactivation...
  5. pmc Characterization of the East Asian variant of aldehyde dehydrogenase-2: bioactivation of nitroglycerin and effects of Alda-1
    Matteo Beretta
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, 8010 Graz, Austria
    J Biol Chem 285:943-52. 2010
    ..In addition, our data revealed an unexpected discrepancy between GTN reductase activity and sGC activation, suggesting that GTN denitration and bioactivation may reflect independent pathways of ALDH2-catalyzed GTN biotransformation...
  6. pmc Role of the general base Glu-268 in nitroglycerin bioactivation and superoxide formation by aldehyde dehydrogenase-2
    M Verena Wenzl
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, A 8010 Graz, Austria
    J Biol Chem 284:19878-86. 2009
    ..ALDH2-catalyzed superoxide formation may essentially contribute to oxidative stress in GTN-exposed blood vessels...
  7. ncbi request reprint Potent inhibition of aldehyde dehydrogenase-2 by diphenyleneiodonium: focus on nitroglycerin bioactivation
    Regina Neubauer
    Department of Pharmacology and Toxicology, Karl Franzens Universität Graz, Austria
    Mol Pharmacol 84:407-14. 2013
    ..In addition, the data provide new evidence for the essential role of ALDH2 in GTN bioactivation and may have implications to other fields of ALDH2 research, such as hepatic ethanol metabolism and cardiac ischemia/reperfusion injury. ..
  8. ncbi request reprint Tetrahydrobiopterin protects soluble guanylate cyclase against oxidative inactivation
    Kurt Schmidt
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Graz, Austria
    Mol Pharmacol 82:420-7. 2012
    ..Protection of sGC against oxidative inactivation may contribute to the known beneficial effects of BH4 in cardiovascular disorders associated with oxidative stress...
  9. pmc Partially irreversible inactivation of mitochondrial aldehyde dehydrogenase by nitroglycerin
    Matteo Beretta
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, A 8010 Graz, Austria
    J Biol Chem 283:30735-44. 2008
    ..These observations are consistent with a significant role for irreversible inactivation of ALDH2 in the development of nitrate tolerance...
  10. ncbi request reprint Bioactivation of nitroglycerin by ascorbate
    Alexander Kollau
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
    Mol Pharmacol 72:191-6. 2007
    ..This reaction may contribute to GTN bioactivation in blood vessels under conditions of GTN tolerance and ascorbate supplementation...
  11. ncbi request reprint Tetrahydrobiopterin as combined electron/proton donor in nitric oxide biosynthesis: cryogenic UV-Vis and EPR detection of reaction intermediates
    Antonius C F Gorren
    Department of Pharmacology and Toxicology, Karl Franzens University Graz, Austria
    Methods Enzymol 396:456-66. 2005
    ..In this chapter, the suitability, drawbacks, and advantages of cryogenic methods are discussed...
  12. pmc Effects of nitroglycerin/L-cysteine on soluble guanylate cyclase: evidence for an activation/inactivation equilibrium controlled by nitric oxide binding and haem oxidation
    Antonius C F Gorren
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, A 8010 Graz, Austria
    Biochem J 390:625-31. 2005
    ..These results suggest that the effect of GTN/Cys is the result of an activation/inactivation equilibrium that is controlled by the rate of NO release and haem oxidation...
  13. pmc Efficient nitrosation of glutathione by nitric oxide
    Bernd Kolesnik
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, A 8010 Graz, Austria
    Free Radic Biol Med 63:51-64. 2013
    ..These observations suggest that direct aerobic nitrosation may represent a physiologically relevant pathway of nitrosothiol formation...
  14. pmc Interaction between neuronal nitric-oxide synthase and tetrahydrobiopterin revisited: studies on the nature and mechanism of tight pterin binding
    Christian L Heine
    Department of Pharmacology and Toxicology, Institute of Pharmaceutical Sciences, Karl Franzens University Graz, A 8010, Graz, Austria
    Biochemistry 53:1284-95. 2014
    ..We propose that incubation of pterin-free nNOS with BH4 creates one tight pterin-binding site per dimer, leaving the other site unaffected, in a reaction that involves redox chemistry. ..
  15. ncbi request reprint Redox role for tetrahydrobiopterin in nitric oxide synthase catalysis: low-temperature optical absorption spectral detection
    Antonius C F Gorren
    Institute for Pharmacology and Toxicology, Karl Franzens Universitat Graz, A 8010 Graz, Austria
    Methods Enzymol 353:114-21. 2002
  16. ncbi request reprint Inefficient spin trapping of superoxide in the presence of nitric-oxide: implications for studies on nitric-oxide synthase uncoupling
    Marc Pignitter
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, A 8010 Graz, Austria
    Free Radic Biol Med 41:455-63. 2006
    ..In addition, the similar behavior of nNOS and SIN-1 provides further evidence for NO as initial product of the NOS reaction...
  17. pmc Vascular bioactivation of nitroglycerin by aldehyde dehydrogenase-2: reaction intermediates revealed by crystallography and mass spectrometry
    Barbara S Lang
    Department of Pharmacology and Toxicology, University of Graz, 8010 Graz, Austria
    J Biol Chem 287:38124-34. 2012
    ..Our results shed light on the molecular mechanism of the GTN denitration reaction and provide useful information on the structural requirements for high affinity binding of organic nitrates to the catalytic site of ALDH2...
  18. ncbi request reprint Interference of the polyphenol epicatechin with the biological chemistry of nitric oxide- and peroxynitrite-mediated reactions
    Rüdiger Wippel
    Institut für Pharmakologie and Toxikologie, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
    Biochem Pharmacol 67:1285-95. 2004
    ..8 and 0.63 microM, respectively. Our data suggest that at up to 0.1 mM (-)-epicatechin preferentially inhibits NO-related nitration and oxidation reactions without affecting NO synthesis and cyclic GMP signaling...
  19. ncbi request reprint Aerobic nitric oxide-induced thiol nitrosation in the presence and absence of magnesium cations
    Bernd Kolesnik
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, A 8010 Graz, Austria
    Free Radic Biol Med 76:286-98. 2014
    ..We propose that in cells Mg(2+)-stimulated NO/O2-induced nitrosothiol formation may be a physiologically relevant reaction. ..
  20. ncbi request reprint Functional characterization of Glu298Asp mutant human endothelial nitric oxide synthase purified from a yeast expression system
    Regina Golser
    Institut fur Pharmakologie und Toxikologie, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
    Nitric Oxide 8:7-14. 2003
    ..To summarize, we obtained no evidence for altered enzyme function of the eNOS mutant that could explain endothelial dysfunction associated with the E298D polymorphism...
  21. ncbi request reprint S-nitrosation of glutathione by nitric oxide, peroxynitrite, and (*)NO/O(2)(*-)
    Astrid Schrammel
    Institut fur Pharmakologie und Toxikologie, Karl Franzens Universitat Graz, Graz, Austria
    Free Radic Biol Med 34:1078-88. 2003
    ..Our results indicate that concerted generation of (*)NO and O(2)(*)(-) may essentially contribute to nitrosative stress in inflammatory diseases...
  22. pmc Cell type-specific recycling of tetrahydrobiopterin by dihydrofolate reductase explains differential effects of 7,8-dihydrobiopterin on endothelial nitric oxide synthase uncoupling
    Kurt Schmidt
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, A 8010 Graz, Austria Electronic address
    Biochem Pharmacol 90:246-53. 2014
    ..Thus, the differential regulation of eNOS uncoupling in different types of endothelial cells may be explained by striking differences in the apparent BH2 affinity of dihydrofolate reductase...
  23. ncbi request reprint Pharmacological interference with dimerization of human neuronal nitric-oxide synthase expressed in adenovirus-infected DLD-1 cells
    Hans Jörg Habisch
    Institut fur Pharmakologie und Toxikologie, Karl Franzens Universitat Graz, Graz, Austria
    Mol Pharmacol 63:682-9. 2003
    ..As time-dependent inhibitors of NOS dimerization, BBS-1 and related compounds provide a promising strategy to develop a new class of selective and clinically useful NOS inhibitors...
  24. ncbi request reprint Binding of L-arginine and imidazole suggests heterogeneity of rat brain neuronal nitric oxide synthase
    Antonius C F Gorren
    Institut fur Pharmakologie und Toxikologie, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
    Biochemistry 41:7819-29. 2002
    ..We propose that this heterogeneous response of nNOS toward Arg and imidazole is underlying the apparently conflicting results reported in the literature...
  25. ncbi request reprint Tetrahydrobiopterin and nitric oxide: mechanistic and pharmacological aspects
    Ernst R Werner
    Institute for Medical Chemistry and Biochemistry, University of Innsbruck, A 6020 Innsbruck, Austria
    Exp Biol Med (Maywood) 228:1291-302. 2003
    ..In this review, we summarize the current view of how tetrahydrobiopterin functions in the generation of NO and focus on pharmacological aspects of tetrahydrobiopterin availability with emphasis on endothelial function...
  26. ncbi request reprint Evidence of two distinct oxygen complexes of reduced endothelial nitric oxide synthase
    Stéphane Marchal
    INSERM U431, Département Biologie Santé, Université Montpellier II, IFR 122, Place Eugène Bataillon, 34095 Montpellier Cedex 5, France
    J Biol Chem 279:19824-31. 2004
    ..Heme-oxy II, which is the oxygen complex competent for product formation, appears to represent a distinct conformation in which the electronic configuration is essentially locked in the ferric superoxide complex...
  27. ncbi request reprint Single-turnover of nitric-oxide synthase in the presence of 4-amino-tetrahydrobiopterin: proposed role for tetrahydrobiopterin as a proton donor
    Morten Sorlie
    Department of Chemistry and Biotechnology, Agricultural University of Norway, PO Box 5040, N 1432 As, Norway
    J Biol Chem 278:48602-10. 2003
    ..NO and reduced 4-amino-BH4. These results suggest that BH4 donates a proton to Fe(II).O2- during catalysis and that inhibition by 4-amino-BH4 may be due to its inability to support this essential protonation step...
  28. ncbi request reprint CO exchange of the oxyferrous complexes of endothelial nitric-oxide synthase oxygenase domain in the presence of 4-amino-tetrahydrobiopterin
    Stephane Marchal
    INSERM U431, Département Biologie Santé, Universite Montpellier II, IFR 122, Montpellier, France
    J Inorg Biochem 98:1217-22. 2004
    ....
  29. ncbi request reprint Two modes of binding of N-hydroxyguanidines to NO synthases: first evidence for the formation of iron-N-hydroxyguanidine complexes and key role of tetrahydrobiopterin in determining the binding mode
    David Lefevre-Groboillot
    Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques, UMR 8601 CNRS, Universite Paris 5, France
    Biochemistry 42:3858-67. 2003
    ..They suggest that N-hydroxyguanidines could constitute a new class of good ligands of heme proteins...
  30. ncbi request reprint Thermodynamic analysis of L-arginine and N omega-hydroxy-L-arginine binding to nitric oxide synthase
    Henrik Zakariassen
    Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, P O Box 5003, N 1432 Aas, Norway
    Biochim Biophys Acta 1784:806-10. 2008
    ..The changes in heat capacity (DeltaC(p)) are relatively small for binding of both NHA and L-Arg (-53+/-18 and -95+/-23 cal/L mol, respectively), which indicates that hydrophobic interactions contribute little to binding...
  31. pmc Formation of transient oxygen complexes of cytochrome p450 BM3 and nitric oxide synthase under high pressure
    Stephane Marchal
    Institut National de la Sante et de la Recherche Medicale, Montpellier, France
    Biophys J 85:3303-9. 2003
    ..Altogether, high pressure appears to be a useful tool to characterize elementary steps in the reaction cycle of heme-thiolate proteins...
  32. ncbi request reprint Tetrahydrobiopterin binding to aromatic amino acid hydroxylases. Ligand recognition and specificity
    Knut Teigen
    Department of Biomedicine, University of Bergen, Jonas Lies vei 91, 5009 Bergen, Norway
    J Med Chem 47:5962-71. 2004
    ..On the basis of analysis of molecular interaction fields, we discuss the selectivity determinants for each hydroxylase and explain the high-affinity inhibitory effect of 7-tetrahydrobiopterin specifically for PAH...
  33. ncbi request reprint Hunting oxygen complexes of nitric oxide synthase at low temperature and high pressure
    Stephane Marchal
    INSERM U710, Place Eugene Bataillon, Universite Montpellier II, Montpellier, France
    Biochem Biophys Res Commun 338:529-35. 2005
    ..Finally, work in the presence of a BH4 analog indicated that proton transfer to the activated oxygen complex is a second essential function of BH4...