Karin Stiasny

Summary

Affiliation: Medical University of Vienna
Country: Austria

Publications

  1. pmc The membrane-proximal "stem" region increases the stability of the flavivirus E protein postfusion trimer and modulates its structure
    Karin Stiasny
    Department of Virology, Medical University of Vienna, Vienna, Austria
    J Virol 87:9933-8. 2013
  2. pmc Age affects quantity but not quality of antibody responses after vaccination with an inactivated flavivirus vaccine against tick-borne encephalitis
    Karin Stiasny
    Department of Virology, Medical University of Vienna, Vienna, Austria
    PLoS ONE 7:e34145. 2012
  3. doi request reprint Quantitative determination of IgM antibodies reduces the pitfalls in the serodiagnosis of tick-borne encephalitis
    K Stiasny
    Department of Virology, Medical University of Vienna, Vienna, Austria
    J Clin Virol 54:115-20. 2012
  4. pmc Probing the flavivirus membrane fusion mechanism by using monoclonal antibodies
    Karin Stiasny
    Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, AT 1095, Vienna, Austria
    J Virol 81:11526-31. 2007
  5. doi request reprint Characteristics of antibody responses in tick-borne encephalitis vaccination breakthroughs
    Karin Stiasny
    Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, AT 1095, Vienna, Austria
    Vaccine 27:7021-6. 2009
  6. pmc Differences in the postfusion conformations of full-length and truncated class II fusion protein E of tick-borne encephalitis virus
    Karin Stiasny
    Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, A 1095 Vienna, Austria
    J Virol 79:6511-5. 2005
  7. pmc Effect of membrane curvature-modifying lipids on membrane fusion by tick-borne encephalitis virus
    Karin Stiasny
    Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, A 1095 Vienna, Austria
    J Virol 78:8536-42. 2004
  8. doi request reprint Molecular mechanisms of flavivirus membrane fusion
    Karin Stiasny
    Institute of Virology, Medical University of Vienna, AT, Austria
    Amino Acids 41:1159-63. 2011
  9. pmc Involvement of lipids in different steps of the flavivirus fusion mechanism
    Karin Stiasny
    Institute of Virology, University of Vienna, Kinderspitalgasse 15, A 1095 Vienna, Austria
    J Virol 77:7856-62. 2003
  10. pmc Impact of quaternary organization on the antigenic structure of the tick-borne encephalitis virus envelope glycoprotein E
    Stefan Kiermayr
    Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, A 1095 Vienna, Austria
    J Virol 83:8482-91. 2009

Collaborators

Detail Information

Publications29

  1. pmc The membrane-proximal "stem" region increases the stability of the flavivirus E protein postfusion trimer and modulates its structure
    Karin Stiasny
    Department of Virology, Medical University of Vienna, Vienna, Austria
    J Virol 87:9933-8. 2013
    ....
  2. pmc Age affects quantity but not quality of antibody responses after vaccination with an inactivated flavivirus vaccine against tick-borne encephalitis
    Karin Stiasny
    Department of Virology, Medical University of Vienna, Vienna, Austria
    PLoS ONE 7:e34145. 2012
    ..Our work indicates that healthy elderly people are able to produce antibodies in response to vaccination with similar avidity and functional activity as young individuals, albeit at lower titers...
  3. doi request reprint Quantitative determination of IgM antibodies reduces the pitfalls in the serodiagnosis of tick-borne encephalitis
    K Stiasny
    Department of Virology, Medical University of Vienna, Vienna, Austria
    J Clin Virol 54:115-20. 2012
    ....
  4. pmc Probing the flavivirus membrane fusion mechanism by using monoclonal antibodies
    Karin Stiasny
    Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, AT 1095, Vienna, Austria
    J Virol 81:11526-31. 2007
    ..These data provide new insights into the mechanisms of flavivirus fusion inhibition by antibodies and their possible contribution to virus neutralization...
  5. doi request reprint Characteristics of antibody responses in tick-borne encephalitis vaccination breakthroughs
    Karin Stiasny
    Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, AT 1095, Vienna, Austria
    Vaccine 27:7021-6. 2009
    ..The antibody profile of these patients therefore had the characteristics of an anamnestic immune response. In the VBTs analyzed, immunological priming and memory were apparently not sufficient or fast enough to prevent the disease...
  6. pmc Differences in the postfusion conformations of full-length and truncated class II fusion protein E of tick-borne encephalitis virus
    Karin Stiasny
    Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, A 1095 Vienna, Austria
    J Virol 79:6511-5. 2005
    ..These data provide experimental evidence to support the model of a fusion process that leads to the interaction of the stem-anchor region with the fusion peptide loop in the postfusion trimer...
  7. pmc Effect of membrane curvature-modifying lipids on membrane fusion by tick-borne encephalitis virus
    Karin Stiasny
    Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, A 1095 Vienna, Austria
    J Virol 78:8536-42. 2004
    ..These data emphasize, however, the importance of the composition of the target membrane in its interactions with the fusion peptide that are crucial for the initiation of fusion...
  8. doi request reprint Molecular mechanisms of flavivirus membrane fusion
    Karin Stiasny
    Institute of Virology, Medical University of Vienna, AT, Austria
    Amino Acids 41:1159-63. 2011
    ..This includes the identification of a conserved histidine as being part of the pH sensor in the fusion protein that responds to the acidic pH and thus initiates the structural transitions driving fusion...
  9. pmc Involvement of lipids in different steps of the flavivirus fusion mechanism
    Karin Stiasny
    Institute of Virology, University of Vienna, Kinderspitalgasse 15, A 1095 Vienna, Austria
    J Virol 77:7856-62. 2003
    ....
  10. pmc Impact of quaternary organization on the antigenic structure of the tick-borne encephalitis virus envelope glycoprotein E
    Stefan Kiermayr
    Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, A 1095 Vienna, Austria
    J Virol 83:8482-91. 2009
    ..We have thus identified several important factors that influence the antigenicity of the flavivirus E protein and have an impact on the interaction with neutralizing antibodies...
  11. pmc Characterization of a structural intermediate of flavivirus membrane fusion
    Karin Stiasny
    Institute of Virology, Medical University of Vienna, Vienna, Austria
    PLoS Pathog 3:e20. 2007
    ..This sequence of events provides important new insights for understanding the dynamic process of viral membrane fusion...
  12. pmc Aluminum hydroxide influences not only the extent but also the fine specificity and functional activity of antibody responses to tick-borne encephalitis virus in mice
    Juergen Zlatkovic
    Department of Virology, Medical University of Vienna, Vienna, Austria
    J Virol 87:12187-95. 2013
    ..Thus, our data provide evidence that aluminum hydroxide can lead to functionally relevant modulations of antibody fine specificities in addition to its known overall immune enhancement effect. ..
  13. pmc Identification of specific histidines as pH sensors in flavivirus membrane fusion
    Richard Fritz
    Institute of Virology, Medical University of Vienna, 1095 Vienna, Austria
    J Cell Biol 183:353-61. 2008
    ..Conserved histidines located outside this critical interface were found to be completely dispensable for triggering fusion...
  14. pmc Characterization of a membrane-associated trimeric low-pH-induced Form of the class II viral fusion protein E from tick-borne encephalitis virus and its crystallization
    Karin Stiasny
    Institute of Virology, University of Vienna, A1095 Vienna, Austria
    J Virol 78:3178-83. 2004
    ..sE trimers, solubilized with nonionic detergents, yielded three-dimensional crystals suitable for X-ray diffraction analysis...
  15. pmc Cryptic properties of a cluster of dominant flavivirus cross-reactive antigenic sites
    Karin Stiasny
    Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, A 1095 Vienna, Austria
    J Virol 80:9557-68. 2006
    ....
  16. pmc Mechanistic insights into the impairment of memory B cells and antibody production in the elderly
    Judith H Aberle
    Department of Virology, Medical University of Vienna, Vienna, Austria
    Age (Dordr) 35:371-81. 2013
    ..The data suggest that the dramatically lower antibody response in the elderly could only partially be accounted for by the reduced B cell numbers and was strongly correlated with profound functional defects in CD4 help...
  17. ncbi request reprint Entry functions and antigenic structure of flavivirus envelope proteins
    Karin Stiasny
    Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, A1095 Vienna, Austria
    Novartis Found Symp 277:57-65; discussion 65-73, 251-3. 2006
    ..Broadly flavivirus cross-reactive antibodies, however, primarily recognize a site involving residues of the highly conserved fusion peptide loop which is cryptic and largely inaccessible on the surface of native infectious virions...
  18. pmc Membrane interactions of the tick-borne encephalitis virus fusion protein E at low pH
    Karin Stiasny
    Institute of Virology, University of Vienna, A 1095 Vienna, Austria
    J Virol 76:3784-90. 2002
    ..Its stability is apparently maintained by contacts between the ectodomains only and is not dependent on sequence elements in the stem-anchor region as previously assumed...
  19. doi request reprint Flaviviruses and flavivirus vaccines
    Franz X Heinz
    Department of Virology, Medical University of Vienna, Kinderspitalgasse 15, 1095 Vienna, Austria
    Vaccine 30:4301-6. 2012
    ..Currently, several kinds of dengue vaccines are in development, but only one of these candidates (a chimeric dengue-yellow fever live attenuated vaccine) has reached the stage of phase 3 clinical trials...
  20. pmc The unique transmembrane hairpin of flavivirus fusion protein E is essential for membrane fusion
    Richard Fritz
    Department of Virology, Medical University of Vienna, Kinderspitalgasse 15, 1095, Vienna, Austria
    J Virol 85:4377-85. 2011
    ..Our data provide evidence for both intratrimer and intertrimer interactions mediated by the TMDs of E and thus extend the existing models of flavivirus membrane fusion...
  21. pmc Dissection of antibody specificities induced by yellow fever vaccination
    Oksana Vratskikh
    Department of Virology, Medical University of Vienna, Vienna, Austria
    PLoS Pathog 9:e1003458. 2013
    ....
  22. pmc Vaccination and tick-borne encephalitis, central Europe
    Franz X Heinz
    Department of Virology, Medical University of Vienna, Vienna, Austria
    Emerg Infect Dis 19:69-76. 2013
    ..Incidence rates remained high for the nonvaccinated population. The vaccine was effective for persons in all age groups. During 2000-2011 in Austria, ≈4,000 cases of TBE were prevented by vaccination...
  23. pmc Mutational analysis of the zippering reaction during flavivirus membrane fusion
    Karen Pangerl
    Department of Virology, Medical University of Vienna, Kinderspitalgasse 15, 1095 Vienna, Austria
    J Virol 85:8495-501. 2011
    ....
  24. ncbi request reprint Flavivirus membrane fusion
    Karin Stiasny
    Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, A1095 Vienna, Austria
    J Gen Virol 87:2755-66. 2006
    ....
  25. doi request reprint Tick-borne encephalitis (TBE) and hepatitis B nonresponders feature different immunologic mechanisms in response to TBE and influenza vaccination with involvement of regulatory T and B cells and IL-10
    Erika Garner-Spitzer
    Institute for Specific Prophylaxis and Tropical Medicine, Medical University of Vienna, Vienna 1090, Austria
    J Immunol 191:2426-36. 2013
    ..We conclude that immunological pathways of nonresponsiveness follow different patterns depending both on vaccine Ag and genetic predisposition of the vaccinee. ..
  26. pmc Tick-borne encephalitis from eating goat cheese in a mountain region of Austria
    Heidemarie Holzmann
    Clinical Institute of Virology, Medical University of Vienna, Vienna, Austria
    Emerg Infect Dis 15:1671-3. 2009
    ..This outbreak indicates the emergence of ticks and TBEV at increasing altitudes in central Europe and the efficiency of oral transmission of TBEV...
  27. pmc Immunodominance and functional activities of antibody responses to inactivated West Nile virus and recombinant subunit vaccines in mice
    Juergen Zlatkovic
    Department of Virology, Medical University of Vienna, A 1095 Vienna, Austria
    J Virol 85:1994-2003. 2011
    ..Despite its low potency for priming, DIII was an excellent boosting antigen, suggesting novel vaccination strategies that strengthen and focus the antibody response to critical neutralizing sites in DIII...
  28. doi request reprint Flaviviruses and their antigenic structure
    F X Heinz
    Department of Virology, Medical University of Vienna, Kinderspitalgasse 15, A 1095 Vienna, Austria
    J Clin Virol 55:289-95. 2012
    ....
  29. pmc Structure of a flavivirus envelope glycoprotein in its low-pH-induced membrane fusion conformation
    Stéphane Bressanelli
    Virologie Moléculaire and Structurale, CNRS UMR 2472 INRA UMR 1157, IFR 115 Gif sur Yvette, France
    EMBO J 23:728-38. 2004
    ....