Franz X Heinz

Summary

Affiliation: Medical University of Vienna
Country: Austria

Publications

  1. pmc Vaccination and tick-borne encephalitis, central Europe
    Franz X Heinz
    Department of Virology, Medical University of Vienna, Vienna, Austria
    Emerg Infect Dis 19:69-76. 2013
  2. doi request reprint Flaviviruses and their antigenic structure
    F X Heinz
    Department of Virology, Medical University of Vienna, Kinderspitalgasse 15, A 1095 Vienna, Austria
    J Clin Virol 55:289-95. 2012
  3. doi request reprint Flaviviruses and flavivirus vaccines
    Franz X Heinz
    Department of Virology, Medical University of Vienna, Kinderspitalgasse 15, 1095 Vienna, Austria
    Vaccine 30:4301-6. 2012
  4. ncbi request reprint Field effectiveness of vaccination against tick-borne encephalitis
    Franz X Heinz
    Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, AT 1095 Vienna, Austria
    Vaccine 25:7559-67. 2007
  5. pmc Mimicking live flavivirus immunization with a noninfectious RNA vaccine
    Regina M Kofler
    Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, A 1095 Vienna, Austria
    Proc Natl Acad Sci U S A 101:1951-6. 2004
  6. pmc Characterization of a structural intermediate of flavivirus membrane fusion
    Karin Stiasny
    Institute of Virology, Medical University of Vienna, Vienna, Austria
    PLoS Pathog 3:e20. 2007
  7. pmc Identification of specific histidines as pH sensors in flavivirus membrane fusion
    Richard Fritz
    Institute of Virology, Medical University of Vienna, 1095 Vienna, Austria
    J Cell Biol 183:353-61. 2008
  8. pmc Involvement of lipids in different steps of the flavivirus fusion mechanism
    Karin Stiasny
    Institute of Virology, University of Vienna, Kinderspitalgasse 15, A 1095 Vienna, Austria
    J Virol 77:7856-62. 2003
  9. pmc Impact of quaternary organization on the antigenic structure of the tick-borne encephalitis virus envelope glycoprotein E
    Stefan Kiermayr
    Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, A 1095 Vienna, Austria
    J Virol 83:8482-91. 2009
  10. pmc Aluminum hydroxide influences not only the extent but also the fine specificity and functional activity of antibody responses to tick-borne encephalitis virus in mice
    Juergen Zlatkovic
    Department of Virology, Medical University of Vienna, Vienna, Austria
    J Virol 87:12187-95. 2013

Collaborators

Detail Information

Publications49

  1. pmc Vaccination and tick-borne encephalitis, central Europe
    Franz X Heinz
    Department of Virology, Medical University of Vienna, Vienna, Austria
    Emerg Infect Dis 19:69-76. 2013
    ..Incidence rates remained high for the nonvaccinated population. The vaccine was effective for persons in all age groups. During 2000-2011 in Austria, ≈4,000 cases of TBE were prevented by vaccination...
  2. doi request reprint Flaviviruses and their antigenic structure
    F X Heinz
    Department of Virology, Medical University of Vienna, Kinderspitalgasse 15, A 1095 Vienna, Austria
    J Clin Virol 55:289-95. 2012
    ....
  3. doi request reprint Flaviviruses and flavivirus vaccines
    Franz X Heinz
    Department of Virology, Medical University of Vienna, Kinderspitalgasse 15, 1095 Vienna, Austria
    Vaccine 30:4301-6. 2012
    ..Currently, several kinds of dengue vaccines are in development, but only one of these candidates (a chimeric dengue-yellow fever live attenuated vaccine) has reached the stage of phase 3 clinical trials...
  4. ncbi request reprint Field effectiveness of vaccination against tick-borne encephalitis
    Franz X Heinz
    Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, AT 1095 Vienna, Austria
    Vaccine 25:7559-67. 2007
    ..Our data confirm the excellent performance of TBE vaccine under field conditions and provide evidence that, in Austria, about 2800 cases were prevented by vaccination in the years 2000-2006...
  5. pmc Mimicking live flavivirus immunization with a noninfectious RNA vaccine
    Regina M Kofler
    Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, A 1095 Vienna, Austria
    Proc Natl Acad Sci U S A 101:1951-6. 2004
    ....
  6. pmc Characterization of a structural intermediate of flavivirus membrane fusion
    Karin Stiasny
    Institute of Virology, Medical University of Vienna, Vienna, Austria
    PLoS Pathog 3:e20. 2007
    ..This sequence of events provides important new insights for understanding the dynamic process of viral membrane fusion...
  7. pmc Identification of specific histidines as pH sensors in flavivirus membrane fusion
    Richard Fritz
    Institute of Virology, Medical University of Vienna, 1095 Vienna, Austria
    J Cell Biol 183:353-61. 2008
    ..Conserved histidines located outside this critical interface were found to be completely dispensable for triggering fusion...
  8. pmc Involvement of lipids in different steps of the flavivirus fusion mechanism
    Karin Stiasny
    Institute of Virology, University of Vienna, Kinderspitalgasse 15, A 1095 Vienna, Austria
    J Virol 77:7856-62. 2003
    ....
  9. pmc Impact of quaternary organization on the antigenic structure of the tick-borne encephalitis virus envelope glycoprotein E
    Stefan Kiermayr
    Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, A 1095 Vienna, Austria
    J Virol 83:8482-91. 2009
    ..We have thus identified several important factors that influence the antigenicity of the flavivirus E protein and have an impact on the interaction with neutralizing antibodies...
  10. pmc Aluminum hydroxide influences not only the extent but also the fine specificity and functional activity of antibody responses to tick-borne encephalitis virus in mice
    Juergen Zlatkovic
    Department of Virology, Medical University of Vienna, Vienna, Austria
    J Virol 87:12187-95. 2013
    ..Thus, our data provide evidence that aluminum hydroxide can lead to functionally relevant modulations of antibody fine specificities in addition to its known overall immune enhancement effect. ..
  11. pmc Age affects quantity but not quality of antibody responses after vaccination with an inactivated flavivirus vaccine against tick-borne encephalitis
    Karin Stiasny
    Department of Virology, Medical University of Vienna, Vienna, Austria
    PLoS ONE 7:e34145. 2012
    ..Our work indicates that healthy elderly people are able to produce antibodies in response to vaccination with similar avidity and functional activity as young individuals, albeit at lower titers...
  12. pmc Mechanistic insights into the impairment of memory B cells and antibody production in the elderly
    Judith H Aberle
    Department of Virology, Medical University of Vienna, Vienna, Austria
    Age (Dordr) 35:371-81. 2013
    ..The data suggest that the dramatically lower antibody response in the elderly could only partially be accounted for by the reduced B cell numbers and was strongly correlated with profound functional defects in CD4 help...
  13. pmc Two distinct size classes of immature and mature subviral particles from tick-borne encephalitis virus
    Steven L Allison
    Institute of Virology, University of Vienna, A 1095 Vienna, Austria
    J Virol 77:11357-66. 2003
    ..These proteins thus appear to have the intrinsic ability to form alternative assembly products that could provide important clues about the role of lateral envelope protein interactions in flavivirus assembly...
  14. pmc Selection and analysis of mutations in an encephalomyocarditis virus internal ribosome entry site that improve the efficiency of a bicistronic flavivirus construct
    Klaus K Orlinger
    Clinical Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, A 1095, Vienna, Austria
    J Virol 81:12619-29. 2007
    ....
  15. pmc Characterization of a membrane-associated trimeric low-pH-induced Form of the class II viral fusion protein E from tick-borne encephalitis virus and its crystallization
    Karin Stiasny
    Institute of Virology, University of Vienna, A1095 Vienna, Austria
    J Virol 78:3178-83. 2004
    ..sE trimers, solubilized with nonionic detergents, yielded three-dimensional crystals suitable for X-ray diffraction analysis...
  16. doi request reprint Effect of pre-existing anti-tick-borne encephalitis virus immunity on neutralising antibody response to the Vero cell-derived, inactivated Japanese encephalitis virus vaccine candidate IC51
    Elisabeth Schuller
    Intercell AG, Vienna Biocenter 6, Vienna, Austria
    Vaccine 26:6151-6. 2008
    ..0001) but not after the second vaccination with IC51. Thus, pre-existing vaccine-induced TBE immunity enhances the neutralising JEV-specific antibody response after a single IC51 vaccination...
  17. doi request reprint Molecular mechanisms of flavivirus membrane fusion
    Karin Stiasny
    Institute of Virology, Medical University of Vienna, AT, Austria
    Amino Acids 41:1159-63. 2011
    ..This includes the identification of a conserved histidine as being part of the pH sensor in the fusion protein that responds to the acidic pH and thus initiates the structural transitions driving fusion...
  18. ncbi request reprint Entry functions and antigenic structure of flavivirus envelope proteins
    Karin Stiasny
    Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, A1095 Vienna, Austria
    Novartis Found Symp 277:57-65; discussion 65-73, 251-3. 2006
    ..Broadly flavivirus cross-reactive antibodies, however, primarily recognize a site involving residues of the highly conserved fusion peptide loop which is cryptic and largely inaccessible on the surface of native infectious virions...
  19. doi request reprint Characteristics of antibody responses in tick-borne encephalitis vaccination breakthroughs
    Karin Stiasny
    Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, AT 1095, Vienna, Austria
    Vaccine 27:7021-6. 2009
    ..The antibody profile of these patients therefore had the characteristics of an anamnestic immune response. In the VBTs analyzed, immunological priming and memory were apparently not sufficient or fast enough to prevent the disease...
  20. pmc Effect of membrane curvature-modifying lipids on membrane fusion by tick-borne encephalitis virus
    Karin Stiasny
    Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, A 1095 Vienna, Austria
    J Virol 78:8536-42. 2004
    ..These data emphasize, however, the importance of the composition of the target membrane in its interactions with the fusion peptide that are crucial for the initiation of fusion...
  21. ncbi request reprint Heterologous gene expression by infectious and replicon vectors derived from tick-borne encephalitis virus and direct comparison of this flavivirus system with an alphavirus replicon
    Rainer Gehrke
    Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, A 1095 Vienna, Austria
    J Gen Virol 86:1045-53. 2005
    ..The results indicate that the VEEV vector is superior with respect to achieving high expression levels, but the TBEV system may be advantageous for applications that require a moderate, but more enduring, gene expression...
  22. pmc Cryptic properties of a cluster of dominant flavivirus cross-reactive antigenic sites
    Karin Stiasny
    Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, A 1095 Vienna, Austria
    J Virol 80:9557-68. 2006
    ....
  23. pmc Isolation of capsid protein dimers from the tick-borne encephalitis flavivirus and in vitro assembly of capsid-like particles
    Stefan Kiermayr
    Institute of Virology, Medical University of Vienna, Vienna, Austria
    J Virol 78:8078-84. 2004
    ..Particulate structures could also be obtained when single-stranded DNA oligonucleotides were used. These data suggest that the dimeric capsid protein functions as a basic building block in the assembly process of flaviviruses...
  24. ncbi request reprint Flavivirus structure and membrane fusion
    Franz X Heinz
    Institute of Virology, University of Vienna, A 1095 Vienna, Austria
    Adv Virus Res 59:63-97. 2003
  25. pmc Tick-borne encephalitis from eating goat cheese in a mountain region of Austria
    Heidemarie Holzmann
    Clinical Institute of Virology, Medical University of Vienna, Vienna, Austria
    Emerg Infect Dis 15:1671-3. 2009
    ..This outbreak indicates the emergence of ticks and TBEV at increasing altitudes in central Europe and the efficiency of oral transmission of TBEV...
  26. pmc Spontaneous mutations restore the viability of tick-borne encephalitis virus mutants with large deletions in protein C
    Regina M Kofler
    Institute of Virology, University of Vienna, Austria
    J Virol 77:443-51. 2003
    ....
  27. pmc Differences in the postfusion conformations of full-length and truncated class II fusion protein E of tick-borne encephalitis virus
    Karin Stiasny
    Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, A 1095 Vienna, Austria
    J Virol 79:6511-5. 2005
    ..These data provide experimental evidence to support the model of a fusion process that leads to the interaction of the stem-anchor region with the fusion peptide loop in the postfusion trimer...
  28. ncbi request reprint Cleavage of protein prM is necessary for infection of BHK-21 cells by tick-borne encephalitis virus
    Sigrid Elshuber
    Institute of Virology, University of Vienna, Kinderspitalgasse 15, A 1095 Vienna, Austria
    J Gen Virol 84:183-91. 2003
    ..Moreover, the results illustrate that mutation of the furin cleavage site is a convenient way to produce single-round infectious flavivirus particles, which may be useful in vaccine and vector development...
  29. pmc Probing the flavivirus membrane fusion mechanism by using monoclonal antibodies
    Karin Stiasny
    Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, AT 1095, Vienna, Austria
    J Virol 81:11526-31. 2007
    ..These data provide new insights into the mechanisms of flavivirus fusion inhibition by antibodies and their possible contribution to virus neutralization...
  30. pmc Dissection of antibody specificities induced by yellow fever vaccination
    Oksana Vratskikh
    Department of Virology, Medical University of Vienna, Vienna, Austria
    PLoS Pathog 9:e1003458. 2013
    ....
  31. doi request reprint Tick-borne encephalitis (TBE) and hepatitis B nonresponders feature different immunologic mechanisms in response to TBE and influenza vaccination with involvement of regulatory T and B cells and IL-10
    Erika Garner-Spitzer
    Institute for Specific Prophylaxis and Tropical Medicine, Medical University of Vienna, Vienna 1090, Austria
    J Immunol 191:2426-36. 2013
    ..We conclude that immunological pathways of nonresponsiveness follow different patterns depending both on vaccine Ag and genetic predisposition of the vaccinee. ..
  32. pmc The membrane-proximal "stem" region increases the stability of the flavivirus E protein postfusion trimer and modulates its structure
    Karin Stiasny
    Department of Virology, Medical University of Vienna, Vienna, Austria
    J Virol 87:9933-8. 2013
    ....
  33. ncbi request reprint Tick-borne encephalitis in childhood--consensus 2004
    Ursula Kunze
    Institute of Social Medicine, Medical University Vienna, Vienna, Austria
    Wien Med Wochenschr 154:242-5. 2004
    ..Therefore immunization should be offered to all children living in or traveling to endemic areas...
  34. ncbi request reprint Role of heparan sulfate for attachment and entry of tick-borne encephalitis virus
    Helga Kroschewski
    Institute of Virology, University of Vienna, A 1095 Vienna, Austria
    Virology 308:92-100. 2003
    ..Thus, even though HS is a major determinant of TBE virus attachment on HS-expressing cells, our findings suggest the existence of an alternative host cell receptor that is less abundant than HS...
  35. pmc Mutational analysis of the zippering reaction during flavivirus membrane fusion
    Karen Pangerl
    Department of Virology, Medical University of Vienna, Kinderspitalgasse 15, 1095 Vienna, Austria
    J Virol 85:8495-501. 2011
    ....
  36. pmc The unique transmembrane hairpin of flavivirus fusion protein E is essential for membrane fusion
    Richard Fritz
    Department of Virology, Medical University of Vienna, Kinderspitalgasse 15, 1095, Vienna, Austria
    J Virol 85:4377-85. 2011
    ..Our data provide evidence for both intratrimer and intertrimer interactions mediated by the TMDs of E and thus extend the existing models of flavivirus membrane fusion...
  37. ncbi request reprint Molecular aspects of TBE virus research
    Franz X Heinz
    Institute of Virology, University of Vienna, Kinderspitalgasse 15, A 1095 Vienna, Austria
    Vaccine 21:S3-S10. 2003
  38. pmc Incorporation of tick-borne encephalitis virus replicons into virus-like particles by a packaging cell line
    Rainer Gehrke
    Institute of Virology, University of Vienna, Vienna, Austria
    J Virol 77:8924-33. 2003
    ..CHO-ME cells can thus be used both as a source for mature TBE virus RSPs and as a safe and convenient replicon packaging cell line, providing the TBE virus surface proteins prM/M and E in trans...
  39. ncbi request reprint Flavivirus membrane fusion
    Karin Stiasny
    Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, A1095 Vienna, Austria
    J Gen Virol 87:2755-66. 2006
    ....
  40. pmc Immunodominance and functional activities of antibody responses to inactivated West Nile virus and recombinant subunit vaccines in mice
    Juergen Zlatkovic
    Department of Virology, Medical University of Vienna, A 1095 Vienna, Austria
    J Virol 85:1994-2003. 2011
    ..Despite its low potency for priming, DIII was an excellent boosting antigen, suggesting novel vaccination strategies that strengthen and focus the antibody response to critical neutralizing sites in DIII...
  41. ncbi request reprint Dynamics of antigenic and genetic changes in the hemagglutinins of influenza A/H3N2 viruses of three consecutive seasons (2002/2003 to 2004/2005) in Austria
    Monika Redlberger
    Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, A 1095 Vienna, Austria
    Vaccine 25:6061-9. 2007
    ..Our results underscore the value of monitoring seasonal influenza strain dynamics by sequence analysis as an instrument that can provide important and timely information on the appearance of strains with epidemiologic significance...
  42. pmc Capsid protein C of tick-borne encephalitis virus tolerates large internal deletions and is a favorable target for attenuation of virulence
    Regina M Kofler
    Institute of Virology, University of Vienna, Vienna, Austria
    J Virol 76:3534-43. 2002
    ....
  43. pmc Membrane interactions of the tick-borne encephalitis virus fusion protein E at low pH
    Karin Stiasny
    Institute of Virology, University of Vienna, A 1095 Vienna, Austria
    J Virol 76:3784-90. 2002
    ..Its stability is apparently maintained by contacts between the ectodomains only and is not dependent on sequence elements in the stem-anchor region as previously assumed...
  44. ncbi request reprint Dengue virus: molecular basis of cell entry and pathogenesis, 25-27 June 2003, Vienna, Austria
    Scott B Halstead
    Pediatric Dengue Vaccine Initiative, 5824 Edson Lane, Rockville, MD 20852, USA
    Vaccine 23:849-56. 2005
    ..Achievement of this aim will require a better understanding of the basic mechanisms by which dengue viruses recognize, attach, enter and infect relevant human cells and how antibodies protect against dengue infections...
  45. pmc Structure of a flavivirus envelope glycoprotein in its low-pH-induced membrane fusion conformation
    Stéphane Bressanelli
    Virologie Moléculaire and Structurale, CNRS UMR 2472 INRA UMR 1157, IFR 115 Gif sur Yvette, France
    EMBO J 23:728-38. 2004
    ....
  46. pmc Folding and dimerization of tick-borne encephalitis virus envelope proteins prM and E in the endoplasmic reticulum
    Ivo C Lorenz
    Institute of Biochemistry, Swiss Federal Institute of Technology, CH 8093 Zurich, Switzerland
    J Virol 76:5480-91. 2002
    ....
  47. ncbi request reprint Report of the Meningitis Program of the International Scientific Working Group on TBE. Serological screening of patients with viral CNS-infection of unknown etiology in search of undiagnosed TBE cases
    Mats Haglund
    Department of Infectious Diseases, Kalmar County Hospital, SE 391 85 Kalmar, Sweden
    Vaccine 21:S66-72. 2003
    ..At least four of those patients were vaccinated against yellow fever. The probable reason for the reactivity seen in these individuals is the well-known cross-reactivity existing among flaviviruses...
  48. ncbi request reprint Serological evidence for tick-borne encephalitis, borreliosis, and human granulocytic anaplasmosis in Mongolia
    Gernot Walder
    Department of Hygiene, Microbiology and Social Medicine, Innsbruck Medical University, Fritz Pregl Str 3, A 6020 Innsbruck, Austria
    Int J Med Microbiol 296:69-75. 2006
    ..Seroprevalence against A. phagocytophilum was 2.3% in Selenge province, 5.6% in Bulgan province, 2.8% in Dornogov province, and 3.0% in Tov province and Ulaanbaatar. We conclude that all three pathogens are endemic in Mongolia...
  49. pmc Intracellular assembly and secretion of recombinant subviral particles from tick-borne encephalitis virus
    Ivo C Lorenz
    Institute of Biochemistry, Swiss Federal Institute of Technology, CH 8093 Zurich, Switzerland
    J Virol 77:4370-82. 2003
    ..Moreover, the carbohydrate side chain of E is involved in at least one assembly or transport step...