Thomas Haller

Summary

Affiliation: Innsbruck Medical University
Location: Innsbruck, Austria

Publications

  1. pmc Interfacial sensing by alveolar type II cells: a new concept in lung physiology?
    Andrea Ravasio
    Dept of Physiology and Medical Physics, Division of Physiology, Innsbruck Medical University, Fritz Pregl Strasse 3, Innsbruck, Austria
    Am J Physiol Cell Physiol 300:C1456-65. 2011
  2. pmc Interfacial stress affects rat alveolar type II cell signaling and gene expression
    Nina Hobi
    Department of Physiology and Medical Physics, Division of Physiology, Innsbruck Medical University, Austria
    Am J Physiol Lung Cell Mol Physiol 303:L117-29. 2012
  3. ncbi request reprint A fluorescent microplate assay for exocytosis in alveolar type II cells
    A Wemhoner
    Department of Pediatrics, Division of Neonatology, Medical University of Innsbruck, Innsbruck, Austria
    J Biomol Screen 11:286-95. 2006
  4. pmc Optical measurement of surface tension in a miniaturized air-liquid interface and its application in lung physiology
    C Bertocchi
    Department of Physiology and Medical Physics, Medical University of Innsbruck, Austria
    Biophys J 89:1353-61. 2005
  5. ncbi request reprint Exocytosis of lung surfactant: from the secretory vesicle to the air-liquid interface
    Paul Dietl
    Department of General Physiology, University of Ulm, Ulm, D 89069, Germany
    Annu Rev Physiol 67:595-621. 2005
  6. ncbi request reprint Ca2+ induced surfactant secretion in alveolar type II cultures isolated from the H-2Kb-tsA58 transgenic mouse
    Paul Jennings
    Department of Physiology, Innsbruck Medical University, Innsbruck, Austria
    Cell Physiol Biochem 15:159-66. 2005
  7. ncbi request reprint Inhibition by cytoplasmic nucleotides of a new cation channel in freshly isolated human and rat type II pneumocytes
    Norbert Mair
    Department of Physiology, Medical University of Innsbruck, Fritz Pregl Str 3, A 6020 Innsbruck, Austria
    Am J Physiol Lung Cell Mol Physiol 287:L1284-92. 2004
  8. ncbi request reprint Pulmonary consequences of a deep breath revisited
    Paul Dietl
    Department of Physiology, Medical University of Innsbruck, Innsbruck, Austria
    Biol Neonate 85:299-304. 2004
  9. ncbi request reprint Tracing surfactant transformation from cellular release to insertion into an air-liquid interface
    T Haller
    Dept of Physiology, Univ of Innsbruck, Fritz Pregl Str 3, A 6020 Innsbruck, Austria
    Am J Physiol Lung Cell Mol Physiol 286:L1009-15. 2004
  10. ncbi request reprint Reduction of intracellular pH inhibits constitutive expression of cyclooxygenase-2 in human colon cancer cells
    Daniela Pirkebner
    Tyrolean Cancer Research Institute, Innrain 66, Innsbruck, Austria
    J Cell Physiol 198:295-301. 2004

Collaborators

Detail Information

Publications45

  1. pmc Interfacial sensing by alveolar type II cells: a new concept in lung physiology?
    Andrea Ravasio
    Dept of Physiology and Medical Physics, Division of Physiology, Innsbruck Medical University, Fritz Pregl Strasse 3, Innsbruck, Austria
    Am J Physiol Cell Physiol 300:C1456-65. 2011
    ..Moreover, they also support a general new concept of mechanosensation in the lung...
  2. pmc Interfacial stress affects rat alveolar type II cell signaling and gene expression
    Nina Hobi
    Department of Physiology and Medical Physics, Division of Physiology, Innsbruck Medical University, Austria
    Am J Physiol Lung Cell Mol Physiol 303:L117-29. 2012
    ..We conclude that alveolar type II cells, due to their location and morphology, are specific sensors of the I(AL), but largely protected from interfacial stress by surfactant release...
  3. ncbi request reprint A fluorescent microplate assay for exocytosis in alveolar type II cells
    A Wemhoner
    Department of Pediatrics, Division of Neonatology, Medical University of Innsbruck, Innsbruck, Austria
    J Biomol Screen 11:286-95. 2006
    ..The authors conclude that the inhibitory effect exerted by phospholipids might be due to action on a later step in exocytosis, probably associated with exocytotic fusion pore expansion and content release out of fused vesicles...
  4. pmc Optical measurement of surface tension in a miniaturized air-liquid interface and its application in lung physiology
    C Bertocchi
    Department of Physiology and Medical Physics, Medical University of Innsbruck, Austria
    Biophys J 89:1353-61. 2005
    ..The present investigation indicates that surface film formation by adsorption of large surfactant aggregates is an important mechanism by which gamma is reduced in the lung...
  5. ncbi request reprint Exocytosis of lung surfactant: from the secretory vesicle to the air-liquid interface
    Paul Dietl
    Department of General Physiology, University of Ulm, Ulm, D 89069, Germany
    Annu Rev Physiol 67:595-621. 2005
    ..We also discuss the fate of secreted material until it is rearranged into units that finally function to reduce the surface tension in the lung...
  6. ncbi request reprint Ca2+ induced surfactant secretion in alveolar type II cultures isolated from the H-2Kb-tsA58 transgenic mouse
    Paul Jennings
    Department of Physiology, Innsbruck Medical University, Innsbruck, Austria
    Cell Physiol Biochem 15:159-66. 2005
    ..However at present very little is known about the regulation of surfactant exocytosis in murine alveolar type II (AT II) cells...
  7. ncbi request reprint Inhibition by cytoplasmic nucleotides of a new cation channel in freshly isolated human and rat type II pneumocytes
    Norbert Mair
    Department of Physiology, Medical University of Innsbruck, Fritz Pregl Str 3, A 6020 Innsbruck, Austria
    Am J Physiol Lung Cell Mol Physiol 287:L1284-92. 2004
    ..RT-PCR confirmed expression of TRPM4b, a channel with functional characteristics almost identical with NSC(Ca/AMP). Potential physiological roles are discussed...
  8. ncbi request reprint Pulmonary consequences of a deep breath revisited
    Paul Dietl
    Department of Physiology, Medical University of Innsbruck, Innsbruck, Austria
    Biol Neonate 85:299-304. 2004
    ..Here we discuss the implication of this additional pool for strain-induced surfactant secretion and propose a revised model for the sequence of events following a single deep breath...
  9. ncbi request reprint Tracing surfactant transformation from cellular release to insertion into an air-liquid interface
    T Haller
    Dept of Physiology, Univ of Innsbruck, Fritz Pregl Str 3, A 6020 Innsbruck, Austria
    Am J Physiol Lung Cell Mol Physiol 286:L1009-15. 2004
    ..They further suggest that transformation of LBPs is a self-regulated interfacial process that most likely does not require structural intermediates or enzymatic activation...
  10. ncbi request reprint Reduction of intracellular pH inhibits constitutive expression of cyclooxygenase-2 in human colon cancer cells
    Daniela Pirkebner
    Tyrolean Cancer Research Institute, Innrain 66, Innsbruck, Austria
    J Cell Physiol 198:295-301. 2004
    ..Defining pHi-mediated mechanisms that govern the constitutive COX-2 expression may help in developing new strategies to block COX-2 over-expression in cancer cells...
  11. ncbi request reprint Ca2+ entry is essential for cell strain-induced lamellar body fusion in isolated rat type II pneumocytes
    Manfred Frick
    Department of Physiology, University of Innsbruck, A 6020, Austria
    Am J Physiol Lung Cell Mol Physiol 286:L210-20. 2004
    ..Both mechanisms result in a graded response of fusions to strain. Our data also allow us to introduce the term "effective strain" as the physiologically relevant portion of the strain amplitude...
  12. doi request reprint High-throughput evaluation of pulmonary surfactant adsorption and surface film formation
    Andrea Ravasio
    Department of Physiology and Medical Physics, Innsbruck Medical University, Innsbruck, Austria
    J Lipid Res 49:2479-88. 2008
    ....
  13. doi request reprint Long-term exposure to LPS enhances the rate of stimulated exocytosis and surfactant secretion in alveolar type II cells and upregulates P2Y2 receptor expression
    Ignacio Garcia-Verdugo
    Department of Physiology and Medical Physics, Innsbruck Medical University, Innsbruck, Austria
    Am J Physiol Lung Cell Mol Physiol 295:L708-17. 2008
    ..We further conclude that chronic exposure to endotoxin sensitizes AT II to increase the extracellular surfactant pool, which aids in the pulmonary host defense mechanisms...
  14. doi request reprint Spatio-temporal aspects, pathways and actions of Ca(2+) in surfactant secreting pulmonary alveolar type II pneumocytes
    Paul Dietl
    Institute of General Physiology, University of Ulm, Albert Einstein Allee 11, 89081 Ulm, Germany
    Cell Calcium 52:296-302. 2012
    ....
  15. pmc Fusion-activated Ca2+ entry via vesicular P2X4 receptors promotes fusion pore opening and exocytotic content release in pneumocytes
    Pika Miklavc
    Institute of General Physiology, University of Ulm, 89081 Ulm, Germany
    Proc Natl Acad Sci U S A 108:14503-8. 2011
    ..Based on these findings, we propose a model for regulation of the exocytotic postfusion phase in nonexcitable cells in which Ca(2+) influx via vesicular Ca(2+) channels regulates fusion pore expansion and vesicle content release...
  16. pmc Effect of exogenous surfactants on viability and DNA synthesis in A549, immortalized mouse type II and isolated rat alveolar type II cells
    Andreas Wemhoner
    Innsbruck Medical University, Department for Pediatrics, Neonatology, Austria
    BMC Pulm Med 11:11. 2011
    ..However, the effect of exogenous surfactant has not been studied directly on alveolar type II cells (ATII cells), a key cell type responsible for alveolar function and repair...
  17. pmc Lamellar bodies form solid three-dimensional films at the respiratory air-liquid interface
    Andrea Ravasio
    Department of Physiology and Medical Physics, Medical University, 6020 Innsbruck, Austria
    J Biol Chem 285:28174-82. 2010
    ..We conclude that surface film formation from LBPs is a highly dynamic and complex process, leading to a more elaborated scenario than that observed and predicted by models using reconstituted, lavaged, or fractionated preparations...
  18. pmc Fusion-activated Ca(2+) entry: an "active zone" of elevated Ca(2+) during the postfusion stage of lamellar body exocytosis in rat type II pneumocytes
    Pika Miklavc
    Institute of General Physiology, University of Ulm, Ulm, Germany
    PLoS ONE 5:e10982. 2010
    ..However, in contrast to the well-known effects of a high cytoplasmic Ca(2+) concentration ([Ca(2+)](c)) in the prefusion phase, the occurrence and significance of Ca(2+) signals in the postfusion phase have not been described before...
  19. pmc Effects of perfluorocarbons on surfactant exocytosis and membrane properties in isolated alveolar type II cells
    Andreas Wemhoner
    University Hospital Dresden, Department for Pediatric Intensive Care and Neonatology, Technical University Dresden, Germany
    Respir Res 11:52. 2010
    ..PFC have been shown to affect various cell types. Thus, effects on alveolar type II (ATII) cells and surfactant metabolism can be expected, data, however, are controversial...
  20. doi request reprint Recent advances in alveolar biology: some new looks at the alveolar interface
    Fred Possmayer
    University of Western Ontario, Departments of Obstetrics Gynaecology and Biochemistry, London, Ontario, Canada
    Respir Physiol Neurobiol 173:S55-64. 2010
    ..This contribution attempts to emphasize how these new approaches have resulted in a fuller appreciation of events presumably occurring at the alveolar interface...
  21. doi request reprint Existence of exocytotic hemifusion intermediates with a lifetime of up to seconds in type II pneumocytes
    Pika Miklavc
    Institute of General Physiology, University of Ulm, Albert Einstein Allee 11, Ulm, D 89081, Germany
    Biochem J 424:7-14. 2009
    ..SLID is a new, non-invasive approach by which a prefusion phase, including hemifusion, can be continuously recorded and distinguished from fusion pore formation and postfusion vesicle swelling...
  22. pmc Mechanical forces impeding exocytotic surfactant release revealed by optical tweezers
    Wolfgang Singer
    Department of Medical Physics, University of Innsbruck, A 6020 Innsbruck, Austria
    Biophys J 84:1344-51. 2003
    ..Strain facilitated surfactant release from preexisting fused vesicles, consistent with the view of mechanical impediments during the release process, which can be overcome by cell strain...
  23. ncbi request reprint Simvastatin inhibits malignant transformation following expression of the Ha-ras oncogene in NIH 3T3 fibroblasts
    Johannes Furst
    Department of Physiology, University of Innsbruck, Fritz Pregl Strasse 3, A 6010 Innsbruck, Austria
    Cell Physiol Biochem 12:19-30. 2002
    ..Thus simvastatin is able to prevent crucial cellular events following expression of the transforming Ha-ras oncogene...
  24. pmc Fusion pore expansion is a slow, discontinuous, and Ca2+-dependent process regulating secretion from alveolar type II cells
    T Haller
    Department of Physiology, University of Innsbruck, A 6020 Innsbruck, Austria
    J Cell Biol 155:279-89. 2001
    ..This study supports a new concept in exocytosis, implicating fusion pores in the regulation of content release for extended periods after initial formation...
  25. ncbi request reprint Effects of bradykinin on NIH 3T3 fibroblasts pretreated with lithium. Mimicking events of Ha-ras oncogene expression
    M Ritter
    Department of Internal Medicine, University of Innsbruck, Austria
    Biochim Biophys Acta 1358:23-30. 1997
    ..However, unlike in cells expressing the Ha-ras oncogene, Li+ pretreatment alone does not allow for growth factor-independent proliferation of the cells...
  26. ncbi request reprint Two different store-operated Ca2+ entry pathways in MDCK cells
    P Dietl
    Department of Physiology, University of Innsbruck, Austria
    Cell Calcium 20:11-9. 1996
    ..We conclude that two Ca2+ entry pathways with different pharmacological and biophysical properties are involved in 'store-operated' Ca2+ entry in MDCK cells...
  27. ncbi request reprint Secretion in alveolar type II cells at the interface of constitutive and regulated exocytosis
    M Frick
    Department of Physiology, University of Innsbruck, Innsbruck, Austria
    Am J Respir Cell Mol Biol 25:306-15. 2001
    ..We propose a common mechanistic model for all types of fusion in this slow secretory cell, in which constitutive and regulated forms of exocytosis are subject to the same principles of regulation...
  28. ncbi request reprint Mechanisms of surfactant exocytosis in alveolar type II cells in vitro and in vivo
    P Dietl
    Department of Physiology, University of Innsbruck, A-6020 Innsbruck, Austria
    News Physiol Sci 16:239-43. 2001
    ..In vitro studies reveal a slow, unique exocytotic process at the interface of stimulated and constitutive exocytosis. The exocytotic mechanisms and sites of regulation in vivo, however, are still poorly understood...
  29. ncbi request reprint Signal transduction pathways in directed migration of human monocytes induced by human growth hormone in vitro
    C M Kahler
    Pneumology Service, Department of General Internal Medicine, Faculty of Medicine, University of Innsbruck, Anichstrasse, 35, 6020 Innsbruck, Austria
    Int Immunopharmacol 1:1351-61. 2001
    ..Additionally, phosphatidylinositol 3-kinase and mitogen-activated protein kinase (MAPK) activation seems to be required. This study revealed signalling pathways in monocyte movement toward GH in vitro...
  30. ncbi request reprint A subpopulation of mitochondria prevents cytosolic calcium overload in endothelial cells after cold ischemia/reperfusion
    A Amberger
    D. Swarovski Research Laboratory, Department of Transplant and General Surgery, University Hospital Innsbruck, Anichstrasse 35, A-6020 Innsbruck, Austria
    Transplantation 71:1821-7. 2001
    ..These mitochondria protect cells against cytosolic calcium overload and probably against subsequent cell injury...
  31. ncbi request reprint The conception of fusion pores as rate-limiting structures for surfactant secretion
    T Haller
    Department of Physiology, University of Innsbruck, Fritz Pregl Str 3, A 6020, Innsbruck, Austria
    Comp Biochem Physiol A Mol Integr Physiol 129:227-31. 2001
    ..In this mini-review, we have compiled published data shedding light on a possibly important role of fusion pores during the secretory process in alveolar type II cells...
  32. ncbi request reprint Inhibition of ATP-induced surfactant exocytosis by dihydropyridine (DHP) derivatives: a non-stereospecific, photoactivated effect and independent of L-type Ca2+ channels
    M Frick
    Department of Physiology, University of Innsbruck, Fritz-Pregl-Strasse 3, A-6020 Innsbruck, Austria
    Biochem Pharmacol 61:1161-7. 2001
    ..We conclude that impairment of ATP-induced surfactant secretion by all tested DHPs and alterations of Ca2+ homeostasis by isradipine are photoactivated effects, independent of L-type Ca2+ channels...
  33. ncbi request reprint Persistent fusion pores but transient fusion in alveolar type II cells
    P Dietl
    Department of Physiology, University of Innsbruck, Innsbruck, A 6020, Austria
    Cell Biol Int 24:803-7. 2000
    ..These data suggest that postfusion mechanisms may regulate the amount of secreted surfactant...
  34. ncbi request reprint Threshold calcium levels for lamellar body exocytosis in type II pneumocytes
    T Haller
    Department of Physiology, University of Innsbruck, A 6020 Innsbruck, Austria
    Am J Physiol 277:L893-900. 1999
    ..This was further demonstrated by the fact that exocytosis is significantly blocked by suppression of the ATP-induced Ca(2+) signal below approximately 300 nmol/l. Our results suggest a highly Ca(2+)-sensitive step in LB exocytosis...