G D Straganz

Summary

Affiliation: Graz University of Technology
Country: Austria

Publications

  1. ncbi request reprint Reaction coordinate analysis for beta-diketone cleavage by the non-heme Fe2+-dependent dioxygenase Dke1
    Grit D Straganz
    Contribution from the Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Petersgasse 12, A 8010 Graz, Austria
    J Am Chem Soc 127:12306-14. 2005
  2. pmc Acetylacetone-cleaving enzyme Dke1: a novel C-C-bond-cleaving enzyme from Acinetobacter johnsonii
    Grit D Straganz
    Institute of Biotechnology, Graz University of Technology, Austria
    Biochem J 369:573-81. 2003
  3. ncbi request reprint Electronic substituent effects on the cleavage specificity of a non-heme Fe(2+)-dependent beta-diketone dioxygenase and their mechanistic implications
    Grit D Straganz
    Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Petersgasse 12, A 8010 Graz, Austria
    J Am Chem Soc 126:12202-3. 2004
  4. ncbi request reprint Variations of the 2-His-1-carboxylate theme in mononuclear non-heme FeII oxygenases
    G D Straganz
    Institute of Biotechnology and Biochemical Engineering Graz University of Technology Petersgasse 12 I, 8010 Graz, Austria
    Chembiochem 7:1536-48. 2006
  5. pmc Kinetic and CD/MCD spectroscopic studies of the atypical, three-His-ligated, non-heme Fe2+ center in diketone dioxygenase: the role of hydrophilic outer shell residues in catalysis
    Grit D Straganz
    Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Petersgasse 12, A 8010 Graz, Austria
    Biochemistry 49:996-1004. 2010
  6. doi request reprint Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves beta-diketones
    Stefan Leitgeb
    Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Austria
    FEBS J 276:5983-97. 2009
  7. doi request reprint Biochemical characterization and mutational analysis of the mononuclear non-haem Fe2+ site in Dke1, a cupin-type dioxygenase from Acinetobacter johnsonii
    Stefan Leitgeb
    Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Petersgasse 12 1, A 8010 Graz, Austria
    Biochem J 418:403-11. 2009

Detail Information

Publications7

  1. ncbi request reprint Reaction coordinate analysis for beta-diketone cleavage by the non-heme Fe2+-dependent dioxygenase Dke1
    Grit D Straganz
    Contribution from the Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Petersgasse 12, A 8010 Graz, Austria
    J Am Chem Soc 127:12306-14. 2005
    ....
  2. pmc Acetylacetone-cleaving enzyme Dke1: a novel C-C-bond-cleaving enzyme from Acinetobacter johnsonii
    Grit D Straganz
    Institute of Biotechnology, Graz University of Technology, Austria
    Biochem J 369:573-81. 2003
    ..1 microM and a k(cat) of 8.5 s(-1). A metabolic pathway for acetylacetone degradation and the putative relationship of this novel enzyme to previously described dioxygenases are discussed...
  3. ncbi request reprint Electronic substituent effects on the cleavage specificity of a non-heme Fe(2+)-dependent beta-diketone dioxygenase and their mechanistic implications
    Grit D Straganz
    Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Petersgasse 12, A 8010 Graz, Austria
    J Am Chem Soc 126:12202-3. 2004
    ..The structure-activity correlation for Dke1 strongly suggests that enzymatic bond cleavage takes place via nucleophilic attack to generate a dioxetane, which then decomposes into the carboxylate and alpha-keto-aldehyde products...
  4. ncbi request reprint Variations of the 2-His-1-carboxylate theme in mononuclear non-heme FeII oxygenases
    G D Straganz
    Institute of Biotechnology and Biochemical Engineering Graz University of Technology Petersgasse 12 I, 8010 Graz, Austria
    Chembiochem 7:1536-48. 2006
    ..These recently discovered metallocenters are discussed with respect to their metal-binding properties and the reaction coordinates of the O(2)-dependent conversions they catalyze...
  5. pmc Kinetic and CD/MCD spectroscopic studies of the atypical, three-His-ligated, non-heme Fe2+ center in diketone dioxygenase: the role of hydrophilic outer shell residues in catalysis
    Grit D Straganz
    Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Petersgasse 12, A 8010 Graz, Austria
    Biochemistry 49:996-1004. 2010
    ..Arg80 and Tyr70 are shown to promote O(2) reduction, while Thr107 stabilizes the Fe(II) cofactor...
  6. doi request reprint Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves beta-diketones
    Stefan Leitgeb
    Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Austria
    FEBS J 276:5983-97. 2009
    ....
  7. doi request reprint Biochemical characterization and mutational analysis of the mononuclear non-haem Fe2+ site in Dke1, a cupin-type dioxygenase from Acinetobacter johnsonii
    Stefan Leitgeb
    Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Petersgasse 12 1, A 8010 Graz, Austria
    Biochem J 418:403-11. 2009
    ..Oxidative inactivation of Dke1 was shown to involve conversion of enzyme-bound Fe2+ into Fe3+, which is then released from the metal centre...