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Genomes and Genes | Heddy ZolaSummaryAffiliation: University of Adelaide Country: Australia Publications
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Publications
Medical applications of leukocyte surface molecules--the CD moleculesHeddy Zola
Child Health Research Institute, Women s and Children s Hospital, Adelaide, Australia
Mol Med 12:312-6. 2006..This short review provides a background to the CD molecules and antibodies against them, and summarizes research, diagnostic, and therapeutic applications of antibodies against these molecules...
CD molecules 2006--human cell differentiation moleculesHeddy Zola
Child Health Research Institute and Adelaide University Discipline of Paediatrics, 72 King William Road, North Adelaide, South Australia 5006, Australia
J Immunol Methods 319:1-5. 2007..At that meeting the Council, acting as Nomenclature Committee, approved a number of new CD designations and changes to some pre-existing CD designations, which are summarized in this report...- Human leukocyte differentiation antigens as therapeutic targets: the CD molecules and CD antibodiesH Zola
Child Health Research Institute, 72 King William Road, North Adelaide 5006, Australia
Expert Opin Biol Ther 1:375-83. 2001..The author is responsible for organising the next (8th) HLDA Workshop and invites readers to suggest ways in which the therapeutic relevance of the Workshop may be enhanced... - The human leucocyte differentiation antigens (HLDA) workshops: the evolving role of antibodies in research, diagnosis and therapyHeddy Zola
Leucocyte Biology Laboratory, Child Health Research Institute, North Adelaide, Australia
Cell Res 15:691-4. 2005..We consider what remains to be achieved (including an estimate of the number of leucocyte surface molecules still to be discovered), and how the field can best move forward... - 1D09C3, an mAb specific for MHC-IIHeddy Zola
Women s and Children s Health Research Institute, 72 King William Road, North Adelaide, SA 5006, South Australia
Curr Opin Mol Ther 10:68-74. 2008..In December 2006, positive safety data from two phase I clinical trials were reported. Final phase I data were expected in mid-2007; however, no additional data have been released at the time of publication... - The 8th Human Leucocyte Differentiation Antigens Workshop: potential new CD moleculesH Zola
Child Health Research Institute, Women's and Children's Hospital, and Co-operative Research Centre for Diagnostics, Adelaide, Australia
J Biol Regul Homeost Agents 16:119-24. 2002 - Human Leucocyte Differentiation Antigen nomenclature: update on CD nomenclature. Report of IUIS/WHO SubcommitteeHeddy Zola
Child Health Research Institute and Department of Paediatrics, Women s and Children s Hospital, Adelaide University, Adelaide 5006, Australia
J Immunol Methods 275:1-8. 2003..The major features of the cellular expression patterns are summarised, and a LocusLink accession number provided to enable the reader to access more detailed information through http://www.ncbi.nlm.nih.gov/LocusLink... - CD molecules 2005: human cell differentiation moleculesHeddy Zola
Child Health Research Institute, 72 King William Rd, North Adelaide 5006, South Australia, Australia
Blood 106:3123-6. 2005.... 
Genome-wide identification of human FOXP3 target genes in natural regulatory T cellsTimothy J Sadlon
Molecular Immunology Laboratory, Women s and Children s Health Research Institute, Women s and Children s Hospital, North Adelaide, South Australia, Australia
J Immunol 185:1071-81. 2010....- Expression of toll-like receptors on B lymphocytesPallave Dasari
Child Health Research Institute, 72 King William Road, North Adelaide, SA 5006, Australia
Cell Immunol 236:140-5. 2005..The higher expression of TLR1 and TLR9 on CD5+ B cells compared to CD5- B cells may reflect the role of B1 cells in more primitive, less specific antibody responses... - Monoclonal antibodies to human cell surface antigensAlice Beare
Women s and Children s Health Research Institute, Women s and Children s Hospital, Adelaide, Australia
Curr Protoc Immunol . 2008.... - Detection of low-abundance membrane markers by immunofluorescence--a comparison of alternative high-sensitivity methods and reagentsChristos Mavrangelos
Child Health Research Institute, Women's and Children's Hospital, North Adelaide SA, Australia
J Immunol Methods 289:169-78. 2004..Furthermore, the new fluorophores allow improved simultaneous detection of two low-abundance markers... - Comparison of blood and synovial fluid th17 and novel peptidase inhibitor 16 Treg cell subsets in juvenile idiopathic arthritisRandall H Grose
Women s and Children s Health Research Institute WCHRI, North Adelaide, and the Discipline of Paediatrics, University of Adelaide, Adelaide, Australia
J Rheumatol 39:2021-31. 2012.... - Elevated serum cytokine levels using cytometric bead arrays predict culture-positive infections in childhood oncology patients with febrile neutropeniaShiraz Badurdeen
Child Health Research Institute and CRC for Diagnostics, SA Pathology at Women s and Children s Hospital, North Adelaide, South Australia
J Pediatr Hematol Oncol 34:e36-8. 2012..Furthermore, low risk of bacteraemia may be predicted by a combination of below threshold cytokines and negative clinical examination... - High-sensitivity immunofluorescence/flow cytometry: detection of cytokine receptors and other low-abundance membrane moleculesHeddy Zola
Child Health Research Institute, North Adelaide, Australia
Curr Protoc Cytom . 2004..This unit discusses the underlying principles of high-sensitivity immunofluorescence and provides an excellent series of protocols for the practical detection of as few as 100 target antigen molecules per cell... - Development of CD4+CD25+FoxP3+ regulatory T cells from cord blood hematopoietic progenitor cellsJonathon F Hutton
Molecular Immunology Laboratory, Discipline of Paediatrics, University of Adelaide, North Adelaide, South Australia 5006
J Leukoc Biol 85:445-51. 2009..This manipulable culture system, therefore, generates functional Tregs and provides a system to elucidate the mechanism of Treg development... - The HLDA8 blind panel: findings and conclusionsBernadette Swart
Child Health Research Institute, Women's and Children's Hospital and Department of Paediatrics, University of Adelaide, 72 King William Rd, North Adelaide, 5006, Australia
J Immunol Methods 305:75-83. 2005..Several antibody pairs among those in the blind panel were found to have very similar staining patterns and were therefore compared by immunohistochemical and/or Western blot analyses for identity... - PI16 is expressed by a subset of human memory Treg with enhanced migration to CCL17 and CCL20Ian C Nicholson
Women s and Children s Health Research Institute, 72 King William Road, North Adelaide, South Australia, 5006, Australia
Cell Immunol 275:12-8. 2012..We conclude that PI16 identifies a novel distinct subset of functional memory Treg which can migrate to sites of inflammation and regulate the pro-inflammatory response at those sites... - Cross-reactivity of anti-human chemokine receptor and anti-TNF family antibodies with common marmoset (Callithrix jacchus) leukocytesSvjetlana Kireta
Transplantation Immunology Laboratory and Department of Medicine, University of Adelaide, The Queen Elizabeth Hospital Campus, 28 Woodville Rd, Adelaide, SA 5011, Australia
Cell Immunol 236:115-22. 2005.... - Expression of Toll-like receptors by neonatal leukocytesPallave Dasari
Women s and Children s Health Research Institute, Women s and Children s Hospital, Adelaide, SA, Australia
Pediatr Allergy Immunol 22:221-8. 2011..TLR2, TLR3, TLR4, TLR8 and TLR9 expression by T lymphocytes was detected in adults and neonates. Monocytes and T lymphocytes from neonates are capable, like adults, of recognizing the presence of pathogens through TLR... - Maternal milk regulation of cell infiltration and interleukin 18 in the intestine of suckling rat pupsI A Penttila
Child Health Research Institute, North Adelaide, SA 5006, Australia
Gut 52:1579-86. 2003..Interleukin 18 (IL-18) is a cytokine that mediates early immune events, and drives T cell development. We assessed the role of TGF-beta in mediating mucosal immune development and specifically the effect on endogenous IL-18... - Toll-like receptorsP Dasari
Child Health Research Institute, Women s and Children s Hospital, Adelaide, Australia
J Biol Regul Homeost Agents 22:17-26. 2008..Some of ten toll-like receptors identified in humans are able to recognise several pathogen-associated molecular patterns... - The 8th Human Leucocyte Differentiation Antigens Workshop: conference, Adelaide 2004H Zola
Child Health Research Institute, Women's and Children's Hospital, Adelaide, Australia
J Biol Regul Homeost Agents 19:72. 2005 - Identification of soluble transforming growth factor-beta receptor III (sTbetaIII) in rat milkM Zhang
Cooperative Research Centre for Tissue Growth and Repair, Child Health Research Institute, North Adelaide, South Australia, Australia
Immunol Cell Biol 79:291-7. 2001..These findings suggest that milk sTbetaRIII is functional, and may modulate milk-derived TGF-beta function in the developing intestine... - In silico evaluation of two mass spectrometry-based approaches for the identification of novel human leukocyte cell-surface proteinsI C Nicholson
Co operative Research Centre for Diagnostics, Women s and Children s Hospital, Adelaide, South Australia, Australia
J Leukoc Biol 77:190-8. 2005..The implication of this for the use of these techniques in the discovery of new leukocyte cell-surface proteins is discussed... - Interleukin 2 receptor regulation and IL-2 function in the human infantH Zola
Child Health Research Institute, Adelaide, Australia
Hum Immunol 59:615-24. 1998.... - Markers of cell lineage, differentiation and activationH Zola
Child Health Research Institute, Women s and Children s Hospital, North Adelaide, Australia
J Biol Regul Homeost Agents 14:218-9. 2000..Lineage markers are the main focus in this commentary; the question of activation markers and markers of differentiation state will be considered in a separate paper... - Immune modulation in suckling rat pups by a growth factor extract derived from milk wheyI A Penttila
CRC for Tissue Growth and Repair, Child Health Research Institute, North Adelaide, South Australia
J Dairy Res 68:587-99. 2001..Immune modulation by extracts derived from milk whey could be of potential benefit for formula-fed and pre-term infants in reducing susceptibility to inappropriate activation to food antigens... - The development of antibody responses in the infantH Zola
Child Health Research Institute, Adelaide, South Australia
Immunol Cell Biol 75:587-90. 1997..Thus, the mechanisms required to make high-affinity antibody develop gradually during the first year of life. Possible evolutionary reasons for this are discussed... - Characterisation of the protein composition of peripheral blood mononuclear cell microsomes by SDS-PAGE and mass spectrometryIan C Nicholson
Child Health Research Institute, Adelaide, Australia Cooperative Research Centre for Diagnostics, Australia
J Immunol Methods 305:84-93. 2005..These results show that previously unidentified lymphocyte plasma membrane proteins can be identified using this approach... - Localization of transforming growth factor-beta receptor types I, II, and III in the postnatal rat small intestineM F Zhang
Cooperative Research Centre, for Tissue Growth and Repair, Child Health Research Institute, North Adelaide, South Australia
Pediatr Res 46:657-65. 1999..The pattern of TGF-beta receptor expression changes with age in a manner that may reflect the change in ligand from TGF-beta2 (milk-derived) to TGF-beta1 (endogenously produced)... - Conservation of leukocyte cell surface proteins: implications for the generation of monoclonal antibodies against newly identified leukocyte cell surface proteinsH Zola
Child Health Research Institute, Women's and Children's Hospital, 72 King William Road, North Adelaide 5006, Australia
Tissue Antigens 68:13-8. 2006..The results indicate that chicken antibodies have the potential to be used as an alternative to mouse reagents where human-mouse sequence conservation is high... - Cytoskeletal protein Flightless (Flii) is elevated in chronic and acute human wounds and wound fluid: neutralizing its activity in chronic but not acute wound fluid improves cellular proliferationNadira Ruzehaji
Women s and Children s Health Research Institute, 72 King William Road, North Adelaide, SA, Australia
Eur J Dermatol 22:740-50. 2012..Application of FnAbs to chronic wounds may therefore be a novel approach used to improve the local environment of non-healing wounds and potentially improve healing outcomes... - Isolation, propagation and characterization of cord blood derived CD4+ CD25+ regulatory T cellsSuzanne Bresatz
Discipline of Paediatrics, Adelaide, Australia
J Immunol Methods 327:53-62. 2007..Protocols are presented for propagation of these cells in culture (yielding >80% FOXP3 positive cells) and for their phenotypic and functional characterization... - Characterization of colonic and mesenteric lymph node dendritic cell subpopulations in a murine adoptive transfer model of inflammatory bowel diseaseJohn Karlis
Child Health Research Institute, Women's and Children's Hospital, North Adelaide, Australia
Inflamm Bowel Dis 10:834-47. 2004..Mature intestinal dendritic cells may form a nucleation site for a local T cell response and play an important role in the pathogenesis of IBD... - Reticulin fibres anchor leukaemic blasts in the marrow of patients with acute lymphoblastic leukaemiaShriram V Nath
Department of Haematology Oncology, SA Pathology at WCH, Adelaide, Australia
Med Hypotheses 77:333-5. 2011..Mobilizing the cells by breaking the 'anchoring ropes' could lead to greater exposure to apoptotic signals... - Detection of cytokine receptors by flow cytometryH Zola
Child Health Research Institute, Adelaide, Australia
Curr Protoc Immunol . 2001..Instructions are also provided for titrating MAbs and cytokines, as well as how to evaluate detection reagents such as anti-Ig conjugates and fluorophores... - The Fc receptor for IgG (Fc gamma RII; CD32) on human neonatal B lymphocytesC F Jessup
Child Health Research Institute, Womens and Childrens Hospital, 5006, North Adelaide SA, Australia
Hum Immunol 62:679-85. 2001..This quantitative difference in FcgammaRIIb expression may explain the reduced susceptibility of cord B cells to antibody-mediated inhibition observed in these experiments... - FcgammaRIIb expression on human germinal center B lymphocytesPeter J Macardle
Department of Immunology, Allergy and Arthritis, Flinders Medical Centre and Flinders University of South Australia, Bedford Park, South Australia, Australia
Eur J Immunol 32:3736-44. 2002..Our results cast some doubt on the value of anti-IgG as a model for antigen-antibody complexes in studying human B cell regulation... - Tolerization as a tool for generating novel monoclonal antibodiesFelicity L Holbrook
Department of Paediatrics and CRC for Diagnostics, Child Health Research Institute, Adelaide University, Adelaide, South Australia, Australia
Immunol Cell Biol 80:319-22. 2002..This review details a number of strategies using either complex protein mixtures or purified proteins as tolerogens and subsequent immunization with a closely related immunogen... - An economical 20 litre bench-top fermenterMichael A Thiel
Department of Ophthalmology, Flinders University of South Australia, Adelaide, Australia
Protein Expr Purif 26:14-8. 2002..5mg/litre/day achieved in shake flasks. The fermenter may be particularly suited for research purposes... - Isolation of antigen-specific B cellsAruna P Kodituwakku
Department of Paediatrics, University of Adelaide, Women's and Children's Hospital, North Adelaide, South Australia, Australia
Immunol Cell Biol 81:163-70. 2003..Particular emphasis has been placed on yield and purity; the two most important parameters of any purification method. The most promising isolation methods have used immunomagnetic sorting and multiparametric flow cytometric analysis... - Cytokine receptor expression by solid tumoursJ Ridings
Child Health Research Institute, Women s and Children s Hospital, Adelaide, South Australia
Ther Immunol 2:67-76. 1995..The broad expression of cytokine receptors suggests that the receptor profile of individual tumours should be determined before the application of therapy that involves the administration of cytokines... - Histamine receptors on T lymphocytesN L Perry
Child Health Research Institute, Women's and Children's Hospital, North Adelaide, Australia
J Biol Regul Homeost Agents 19:78-83. 2005 - Direct demonstration of membrane IL-1 alpha on the surface of circulating B lymphocytes and monocytesH Zola
Department of Clinical Immunology, Flinders Medical Centre, Adelaide, South Australia
J Immunol 150:1755-62. 1993..Activation of tonsil B cells with anti-IgM, IL-4, IL-2, and mixtures of these reagents led to small increases in IL-1 alpha expression, as did activation of unfractionated tonsil cells with PHA... - Rapid simultaneous measurement of multiple cytokines using 100 microl sample volumes--association with neonatal sepsisG Hodge
Department of Haematology, Women s and Children s Hospital, North Adelaide, South Australia
Clin Exp Immunol 137:402-7. 2004..In conclusion, neonatal sepsis is associated with the elevation of multiple plasma cytokines. The use of CBA kits is a rapid, easy, low sample volume and sensitive method to measure multiple plasma cytokines... - A comparison of Verotoxin B-subunit (Stx1B) and CD77 antibody to define germinal centre populationsS Bailey
Department of Immunology, Allergy and Arthritis, Flinders Medical Centre and Flinders University, Adelaide, SA, Australia
Cell Immunol 236:167-70. 2005..The strong staining patterns of Stx1B suggest that it may be useful in defining germinal center B cell populations... - Antibody against CD20 in patients with B cell malignancyP J Adamson
Child Health Research Institute, Women's and Children's Hospital, University of Adelaide, 72 King William Road, Adelaide 5006, Australia
Leuk Res 25:1047-50. 2001..To identify a good donor for library construction, it would be advisable to screen donors for antibody against the antigens of interest... - CD antigens 2002David Mason
Blood 99:3877-80. 2002 - Human leucocyte differentiation antigensHeddy Zola
Trends Immunol 24:353-4. 2003 - Reference: CD Antigens 2002David Mason
Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, UK
J Immunol 168:2083-6. 2002 - Development of a cluster of differentiation antibody-based protein microarrayMichael Abdo
CSIRO Division of Health Sciences and Nutrition, 343 Royal Parade, Parkville, Victoria 3052, Australia
J Immunol Methods 305:3-9. 2005.... - CD Antigens 2001David Mason
Haematology Department, John Radcliffe Hospital, Oxford, OX3 9DU, United Kingdom
Mod Pathol 15:71-6. 2002 - Generation of murine monoclonal antibodies to Haemophilus influenzae type b capsular polysaccharide by in vivo immunizationAruna P Kodituwakku
Department of Paediatrics, University of Adelaide
Hybrid Hybridomics 23:160-7. 2004..The anti-PRP antibodies from one hybridoma clone (B10) are further characterized and potential applications are discussed...