Hongyuan Yang


Affiliation: University of New South Wales
Country: Australia


  1. Dong J, Du X, Wang H, Wang J, Lu C, Chen X, et al. Allosteric enhancement of ORP1-mediated cholesterol transport by PI(4,5)P2/PI(3,4)P2. Nat Commun. 2019;10:829 pubmed publisher
    ..Thus, our work unveils a distinct mechanism by which PIPs may allosterically enhance OSBP/ORPs-mediated transport of major lipid species such as cholesterol. ..
  2. Wang H, Ma Q, Qi Y, Dong J, Du X, Rae J, et al. ORP2 Delivers Cholesterol to the Plasma Membrane in Exchange for Phosphatidylinositol 4, 5-Bisphosphate (PI(4,5)P2). Mol Cell. 2019;73:458-473.e7 pubmed publisher
    ..Our results identify a novel pathway for cholesterol delivery to the PM and establish ORP2 as a key regulator of both cholesterol and PI(4,5)P2 of the PM. ..
  3. Yan R, Qian H, Lukmantara I, Gao M, Du X, Yan N, et al. Human SEIPIN Binds Anionic Phospholipids. Dev Cell. 2018;47:248-256.e4 pubmed publisher
    ..Both full-length SEIPIN and its lumenal domain can bind anionic phospholipids including phosphatidic acid. Our results suggest that SEIPIN forms a scaffold that helps maintain phospholipid homeostasis and surface tension of the ER. ..
  4. Gao M, Yang H. VPS13: A lipid transfer protein making contacts at multiple cellular locations. J Cell Biol. 2018;217:3322-3324 pubmed publisher
    ..2018. J. Cell Biol. https://doi.org/10.1083/jcb.201807019) provide biochemical and structural evidence for VPS13 proteins in the nonvesicular transport of phospholipids. ..
  5. Yang H, Galea A, Sytnyk V, Crossley M. Controlling the size of lipid droplets: lipid and protein factors. Curr Opin Cell Biol. 2012;24:509-16 pubmed publisher
    ..Phospholipids appear to have an evolutionarily conserved role in LD growth. Protein factors may regulate LD expansion directly and/or indirectly through modulating the level and composition of phospholipids on LD surface. ..
  6. Du X, Brown A, Yang H. Novel mechanisms of intracellular cholesterol transport: oxysterol-binding proteins and membrane contact sites. Curr Opin Cell Biol. 2015;35:37-42 pubmed publisher
    ..These discoveries highlight the crucial role of MCSs, or junctions, in facilitating lipid movement, and provide mechanistic insights into how cholesterol is sorted in cells. ..
  7. request reprint
    Liu X, Xie B, Qi Y, Du X, Wang S, Zhang Y, et al. The expression of SEIPIN in the mouse central nervous system. Brain Struct Funct. 2016;221:4111-4127 pubmed
    ..The results of this study help to explain the clinical manifestations of patients with Berardinelli-Seip congenital lipodystrophy (Bscl2) gene mutations. ..
  8. Wang M, Gao M, Liao J, Qi Y, Du X, Wang Y, et al. Adipose tissue deficiency results in severe hyperlipidemia and atherosclerosis in the low-density lipoprotein receptor knockout mice. Biochim Biophys Acta. 2016;1861:410-8 pubmed publisher
    ..Our results also reveal a previously unappreciated strong link between adipose tissue and LDLR in plasma cholesterol metabolism. ..
  9. Du X, Zadoorian A, Lukmantara I, Qi Y, Brown A, Yang H. Oxysterol-binding protein-related protein 5 (ORP5) promotes cell proliferation by activation of mTORC1 signaling. J Biol Chem. 2018;293:3806-3818 pubmed publisher
    ..Together, our results suggest that ORP5 expression is positively correlated with mTORC1 signaling and that ORP5 stimulates cell proliferation, at least in part, by activating mTORC1. ..

More Information


  1. Qi Y, Kapterian T, Du X, Ma Q, Fei W, Zhang Y, et al. CDP-diacylglycerol synthases regulate the growth of lipid droplets and adipocyte development. J Lipid Res. 2016;57:767-80 pubmed publisher
    ..We have therefore identified CDS1 and CDS2 as important novel regulators of lipid storage, and these results highlight the crucial role of phospholipids in mammalian lipid storage. ..
  2. Du X, Lukmantara I, Yang H. CRISPR/Cas9-Mediated Generation of Niemann-Pick C1 Knockout Cell Line. Methods Mol Biol. 2017;1583:73-83 pubmed publisher
    ..The protocol details the steps for single guide RNA oligo cloning, cell colony selection, and cell line verification by filipin staining and immunoblotting. ..
  3. Qi Y, Sun L, Yang H. Lipid droplet growth and adipocyte development: mechanistically distinct processes connected by phospholipids. Biochim Biophys Acta Mol Cell Biol Lipids. 2017;1862:1273-1283 pubmed publisher
    ..This article is part of a Special Issue entitled: Recent Advances in Lipid Droplet Biology edited by Rosalind Coleman and Matthijs Hesselink. ..
  4. Du X, Turner N, Yang H. The role of oxysterol-binding protein and its related proteins in cancer. Semin Cell Dev Biol. 2018;81:149-153 pubmed publisher
    ..Here, we summarize recent progress in understanding the role of OSBP/ORPs in cancer development, and discuss how the lipid transfer function of OSBP/ORPs may underpin their role in tumorigenesis. ..
  5. Ghai R, Du X, Wang H, Dong J, Ferguson C, Brown A, et al. ORP5 and ORP8 bind phosphatidylinositol-4, 5-biphosphate (PtdIns(4,5)P 2) and regulate its level at the plasma membrane. Nat Commun. 2017;8:757 pubmed publisher
    ..Here the authors show that PtdIns(4,5)P 2 plays a critical role in the targeting and function of ORP5/8 at the PM. ..
  6. Pagac M, Cooper D, Qi Y, Lukmantara I, Mak H, Wu Z, et al. SEIPIN Regulates Lipid Droplet Expansion and Adipocyte Development by Modulating the Activity of Glycerol-3-phosphate Acyltransferase. Cell Rep. 2016;17:1546-1559 pubmed publisher
    ..These data identify SEIPIN as an evolutionarily conserved regulator of microsomal GPAT and suggest that GPAT inhibitors might be useful for the treatment of human BSCL2 patients. ..