Richard J Whittington

Summary

Affiliation: University of Sydney
Country: Australia

Publications

  1. ncbi In utero infection of cattle with Mycobacterium avium subsp. paratuberculosis: a critical review and meta-analysis
    Richard J Whittington
    Farm Animal and Veterinary Public Health, Faculty of Veterinary Science, University of Sydney, PMB 3 Camden, NSW 2570, Australia
    Vet J 179:60-9. 2009
  2. ncbi Factors affecting isolation and identification of Mycobacterium avium subsp. paratuberculosis from fecal and tissue samples in a liquid culture system
    Richard J Whittington
    Faculty of Veterinary Science, The University of Sydney, New South Wales 2006, Australia
    J Clin Microbiol 47:614-22. 2009
  3. ncbi Herpesvirus that caused epizootic mortality in 1995 and 1998 in pilchard, Sardinops sagax neopilchardus (Steindachner), in Australia is now endemic
    R J Whittington
    Faculty of Veterinary Science, University of Sydney, Sydney, NSW, Australia
    J Fish Dis 31:97-105. 2008
  4. ncbi Global trade in ornamental fish from an Australian perspective: the case for revised import risk analysis and management strategies
    R J Whittington
    Faculty of Veterinary Science, University of Sydney, PMB 3, Camden, NSW 2570, Australia
    Prev Vet Med 81:92-116. 2007
  5. ncbi Thermal inactivation profiles of Mycobacterium avium subsp. paratuberculosis in lamb skeletal muscle homogenate fluid
    Richard J Whittington
    Farm Animal and Veterinary Public Health, Faculty of Veterinary Science, University of Sydney, Australia
    Int J Food Microbiol 137:32-9. 2010
  6. ncbi Survival of Mycobacterium avium subsp. paratuberculosis in dam water and sediment
    Richard J Whittington
    Faculty of Veterinary Science, The University of Sydney, Private Bag 3, Camden, NSW 2570, Australia
    Appl Environ Microbiol 71:5304-8. 2005
  7. ncbi Survival and dormancy of Mycobacterium avium subsp. paratuberculosis in the environment
    Richard J Whittington
    Faculty of Veterinary Science, The University of Sydney, Sydney, Australia
    Appl Environ Microbiol 70:2989-3004. 2004
  8. ncbi Association of farm soil characteristics with ovine Johne's disease in Australia
    Navneet K Dhand
    The University of Sydney, 425 Werombi Road, Camden, NSW 2570, Australia
    Prev Vet Med 89:110-20. 2009
  9. ncbi Transmission of Mycobacterium avium subsp. paratuberculosis: dose-response and age-based susceptibility in a sheep model
    Helen McGregor
    The University of Sydney, 425 Werombi Road, Camden, 2570 NSW, Australia
    Prev Vet Med 107:76-84. 2012
  10. ncbi Optimization of a whole blood gamma interferon assay for the detection of sheep infected with Mycobacterium avium subspecies paratuberculosis
    Katrina L Bosward
    Faculty of Veterinary Science, The University of Sydney, J L Shute Building, 425 Werombi Road, Camden, NSW 2570, Australia
    J Vet Diagn Invest 22:210-7. 2010

Collaborators

Detail Information

Publications50

  1. ncbi In utero infection of cattle with Mycobacterium avium subsp. paratuberculosis: a critical review and meta-analysis
    Richard J Whittington
    Farm Animal and Veterinary Public Health, Faculty of Veterinary Science, University of Sydney, PMB 3 Camden, NSW 2570, Australia
    Vet J 179:60-9. 2009
    ..The consequences of fetal infection for the calves so infected are discussed in the context of diagnosis and vaccination together with recommendations for future research...
  2. ncbi Factors affecting isolation and identification of Mycobacterium avium subsp. paratuberculosis from fecal and tissue samples in a liquid culture system
    Richard J Whittington
    Faculty of Veterinary Science, The University of Sydney, New South Wales 2006, Australia
    J Clin Microbiol 47:614-22. 2009
    ..2%. Liquid medium was more sensitive than solid medium culture for M. avium subsp. paratuberculosis. The applicability of these findings for C strains is discussed...
  3. ncbi Herpesvirus that caused epizootic mortality in 1995 and 1998 in pilchard, Sardinops sagax neopilchardus (Steindachner), in Australia is now endemic
    R J Whittington
    Faculty of Veterinary Science, University of Sydney, Sydney, NSW, Australia
    J Fish Dis 31:97-105. 2008
    ..5), 31 (22-43), 42 (31-55) and 29 (20-41), respectively. PHV is now endemic in Australian populations of pilchard. Implications of the findings for fisheries management are discussed...
  4. ncbi Global trade in ornamental fish from an Australian perspective: the case for revised import risk analysis and management strategies
    R J Whittington
    Faculty of Veterinary Science, University of Sydney, PMB 3, Camden, NSW 2570, Australia
    Prev Vet Med 81:92-116. 2007
    ....
  5. ncbi Thermal inactivation profiles of Mycobacterium avium subsp. paratuberculosis in lamb skeletal muscle homogenate fluid
    Richard J Whittington
    Farm Animal and Veterinary Public Health, Faculty of Veterinary Science, University of Sydney, Australia
    Int J Food Microbiol 137:32-9. 2010
    ..Based on the models used in this study, there is a low probability of survival of MAP provided that red meat is cooked to recommended standards...
  6. ncbi Survival of Mycobacterium avium subsp. paratuberculosis in dam water and sediment
    Richard J Whittington
    Faculty of Veterinary Science, The University of Sydney, Private Bag 3, Camden, NSW 2570, Australia
    Appl Environ Microbiol 71:5304-8. 2005
    ..Survival of the organism in water destined for human consumption will need to be addressed if the organism is found to be involved in the etiology of Crohn's disease...
  7. ncbi Survival and dormancy of Mycobacterium avium subsp. paratuberculosis in the environment
    Richard J Whittington
    Faculty of Veterinary Science, The University of Sydney, Sydney, Australia
    Appl Environ Microbiol 70:2989-3004. 2004
    ..However, survival of M. avium subsp. paratuberculosis in the environment is finite, consistent with its taxonomic description as an obligate parasite of animals...
  8. ncbi Association of farm soil characteristics with ovine Johne's disease in Australia
    Navneet K Dhand
    The University of Sydney, 425 Werombi Road, Camden, NSW 2570, Australia
    Prev Vet Med 89:110-20. 2009
    ..We hypothesise that this is due to adsorption of MAP to clay and the consequent retention of the bacteria in the topsoil, thus making them available in higher numbers to grazing sheep...
  9. ncbi Transmission of Mycobacterium avium subsp. paratuberculosis: dose-response and age-based susceptibility in a sheep model
    Helen McGregor
    The University of Sydney, 425 Werombi Road, Camden, 2570 NSW, Australia
    Prev Vet Med 107:76-84. 2012
    ....
  10. ncbi Optimization of a whole blood gamma interferon assay for the detection of sheep infected with Mycobacterium avium subspecies paratuberculosis
    Katrina L Bosward
    Faculty of Veterinary Science, The University of Sydney, J L Shute Building, 425 Werombi Road, Camden, NSW 2570, Australia
    J Vet Diagn Invest 22:210-7. 2010
    ....
  11. ncbi Experimental infection model for Johne's disease using a lyophilised, pure culture, seedstock of Mycobacterium avium subspecies paratuberculosis
    Douglas J Begg
    Farm Animal and Veterinary Public Health, Faculty of Veterinary Science, University of Sydney, PMB3, Camden, NSW 2570, Australia
    Vet Microbiol 141:301-11. 2010
    ..It is recommended that an international bank of master seedstock be established, containing low passage isolates that are representative of the major strains of Map, S and C...
  12. ncbi A longitudinal study to evaluate the diagnostic potential of a direct faecal quantitative PCR test for Johne's disease in sheep
    Satoko Kawaji
    Faculty of Veterinary Science, The University of Sydney, 425 Werombi Road, Camden, New South Wales 2570, Australia
    Vet Microbiol 148:35-44. 2011
    ..Quantification of MAP DNA in faeces by QPCR may provide immediate information to estimate the stage of the infection as well as the risk of transmission from infected animals...
  13. ncbi Enzyme-linked immunospot: an alternative method for the detection of interferon gamma in Johne's disease
    Douglas J Begg
    Farm Animal and Veterinary Public Health, Faculty of Veterinary Science, University of Sydney, PMB3, Camden 2570, Australia
    J Vet Diagn Invest 21:187-96. 2009
    ....
  14. ncbi Optimisation of culture of Mycobacterium avium subspecies paratuberculosis from blood samples
    Katrina Bower
    Farm Animal and Veterinary Public Health, Faculty of Veterinary Science, University of Sydney, PMB 4003, Narellan, NSW 2567, Australia
    J Microbiol Methods 80:93-9. 2010
    ..The optimised method has an analytical sensitivity of at least 10(1) MAP per ml of spiked whole blood and will enable trials to determine the incidence, duration and magnitude of mycobacteraemia in infected animals and humans...
  15. ncbi Candidate gene and genome-wide association studies of Mycobacterium avium subsp. paratuberculosis infection in cattle and sheep: a review
    Auriol C Purdie
    Farm Animal and Veterinary Public Health, Faculty of Veterinary Sciences University of Sydney, Australia
    Comp Immunol Microbiol Infect Dis 34:197-208. 2011
    ..Here we review the current published evidence for a genetic association with paratuberculosis susceptibility, technological advances that have progressed the field and potential avenues for future research...
  16. ncbi Proteomic profiling of ovine serum by SELDI-TOF MS: optimisation, reproducibility and feasibility of biomarker discovery using routinely collected samples
    Ling Zhong
    Faculty of Veterinary Science, University of Sydney, Camden, NSW 2570, Australia
    Comp Immunol Microbiol Infect Dis 33:47-63. 2010
    ..This information will inform future studies on serum proteome profiling in livestock, but independent assessments are recommended for each species...
  17. ncbi Indoleamine 2,3-dioxygenase, tryptophan catabolism, and Mycobacterium avium subsp. paratuberculosis: a model for chronic mycobacterial infections
    Karren M Plain
    Farm Animal and Veterinary Public Health, University of Sydney, Faculty of Veterinary Science, Camden, NSW 2570, Australia
    Infect Immun 79:3821-32. 2011
    ..These findings raise new questions about the host-mycobacterium interactions in the progression from latent to clinical disease...
  18. ncbi Detection of Mycobacterium avium subsp. paratuberculosis in ovine faeces by direct quantitative PCR has similar or greater sensitivity compared to radiometric culture
    Satoko Kawaji
    Faculty of Veterinary Science, University of Sydney, Camden, NSW, Australia
    Vet Microbiol 125:36-48. 2007
    ..None of the faecal samples from 176 sheep that were not exposed to MAP were positive in QPCR. This is the first report of a direct faecal QPCR assay that has similar sensitivity to a gold standard radiometric culture assay...
  19. ncbi Enhancement of the interferon gamma assay to detect paratuberculosis using interleukin-7 and interleukin-12 potentiation
    Karren M Plain
    Farm Animal and Veterinary Public Health, Faculty of Veterinary Science, University of Sydney, Camden, New South Wales 2570, Australia
    Vet Immunol Immunopathol 149:28-37. 2012
    ..The potentiation protocol allowed for practical shipment of blood samples from farm to laboratory, extending permissible transit times and applicability of IFN-γ testing to detect Johne's disease...
  20. ncbi Expression of genes associated with the antigen presentation and processing pathway are consistently regulated in early Mycobacterium avium subsp. paratuberculosis infection
    Auriol C Purdie
    Farm Animal and Veterinary Public Health, University of Sydney, Camden, Australia
    Comp Immunol Microbiol Infect Dis 35:151-62. 2012
    ..This requires further in-depth analysis since it exposes a hitherto unconfirmed association between MAP exposure and in vivo MHC gene modulation...
  21. ncbi Analysis of the growth pattern, survival and proteome of Mycobacteriumavium subsp. paratuberculosis following exposure to heat
    Sanjeev Gumber
    The University of Sydney, Camden 2570, NSW, Australia
    Vet Microbiol 136:82-90. 2009
    ..These proteins represented a range of metabolic pathways, including 12 previously identified in M. tuberculosis during heat stress. These proteins may be required for the survival of MAP both in the environment and within the host...
  22. ncbi Use of growth indices from radiometric culture for quantification of sheep strains of Mycobacterium avium subsp. paratuberculosis
    Leslie A Reddacliff
    Faculty of Veterinary Science, University of Sydney, Sydney, New South Wales 2570, Australia
    Appl Environ Microbiol 69:3510-6. 2003
    ..Organisms from feces or contaminated soil grew more slowly than those from cultures or tissues, and separate equations were developed for enumeration from these sources...
  23. ncbi Toll-like receptor genes are differentially expressed at the sites of infection during the progression of Johne's disease in outbred sheep
    Deborah L Taylor
    Faculty of Veterinary Science, University of Sydney, PMB3, Camden, NSW 2570, Australia
    Vet Immunol Immunopathol 124:132-51. 2008
    ..Further study of TLR expression in Johne's disease in ruminants is warranted as these observed differences may help explain pathogenesis and may be useful in the future diagnosis of M. ptb infection...
  24. ncbi Estimation of sensitivity and flock-sensitivity of pooled faecal culture for Mycobacterium avium subsp. paratuberculosis in sheep
    Navneet K Dhand
    Faculty of Veterinary Science, The University of Sydney, 425 Werombi Road, Camden, NSW 2570, Australia
    Prev Vet Med 95:248-57. 2010
    ....
  25. ncbi Genomic comparison of Mycobacterium avium subsp. paratuberculosis sheep and cattle strains by microarray hybridization
    Ian B Marsh
    Faculty of Veterinary Science, University of Sydney, Camden, NSW, Australia
    J Bacteriol 188:2290-3. 2006
    ..These deletions may help explain some of the differences in pathogenicity and host specificity observed between the cattle and sheep strains of Mycobacterium avium subsp. paratuberculosis...
  26. ncbi Optimization of Betanodavirus culture and enumeration in striped snakehead fish cells
    Paul Hick
    Faculty of Veterinary Science, University of Sydney, 425 Werrombi Road, Camden NSW 2570 Australia
    J Vet Diagn Invest 23:465-75. 2011
    ..3 logs higher when cells were inoculated less than 36 hr after subculture at less than 80% confluence compared to cells inoculated at greater than 80% confluence and more than 36 hr after subculture (P < 0.05)...
  27. ncbi Evaluation of the immunogenicity of Mycobacterium avium subsp. paratuberculosis (MAP) stress-associated recombinant proteins
    Satoko Kawaji
    Faculty of Veterinary Science, The University of Sydney, 425 Werombi Road, Camden, New South Wales 2570, Australia
    Vet Microbiol 155:298-309. 2012
    ..This is consistent with the hypothesis of latency or dormancy in subclinical mycobacterial infection...
  28. ncbi An evaluation of mycophage therapy, chemotherapy and vaccination for control of Mycobacterium avium subsp. paratuberculosis infection
    David L Emery
    Faculty of Veterinary Science, University of Sydney Locked Bag 3, Camden, NSW 2570, Australia
    Vet Microbiol 104:143-55. 2004
    ..An appraisal of recent experimental results suggests that neonatal vaccination with a defined dose of M. paratuberculosis offers the best prospects for the induction of protective Th1-type immunity...
  29. ncbi Evaluation of radiometric faecal culture and direct PCR on pooled faeces for detection of Mycobacterium avium subsp. paratuberculosis in cattle
    Graeme J Eamens
    Elizabeth Macarthur Agricultural Institute, NSW Department of Primary Industries, Menangle, NSW, Australia
    Vet Microbiol 125:22-35. 2007
    ..The results suggest that for cattle shedding relatively high concentrations of Map in faeces (>2 x 10(5) viable Map/g), maximal dilutions of 1 in 30 for PFC and 1 in 10 for D-PCR would be applicable...
  30. ncbi Growth pattern and partial proteome of Mycobacterium avium subsp. paratuberculosis during the stress response to hypoxia and nutrient starvation
    Sanjeev Gumber
    The University of Sydney, Camden, NSW 2570, Australia
    Vet Microbiol 133:344-57. 2009
    ..These proteins are potential screening targets for future diagnosis, prevention and control of M. a. paratuberculosis infection and their identification will assist understanding the pathogenesis of diseases caused by this organism...
  31. ncbi Evaluation of the immunogenicity of recombinant stress-associated proteins during Mycobacterium avium subsp. paratuberculosis infection: Implications for pathogenesis and diagnosis
    Sanjeev Gumber
    Faculty of Veterinary Science, The University of Sydney, Private Bag 3, Camden 2570, NSW, Australia
    Vet Microbiol 137:290-6. 2009
    ..These data provide fundamental information on host-mycobacterial interactions and have conceptual implications for the development of future diagnostic tests for early immune responses in animals infected with mycobacteria...
  32. ncbi Comparative immunological and microbiological aspects of paratuberculosis as a model mycobacterial infection
    Richard J Whittington
    The University of Sydney, Faculty of Veterinary Science, Camden, NSW 2570, Australia
    Vet Immunol Immunopathol 148:29-47. 2012
    ....
  33. ncbi In silico identification of epitopes in Mycobacterium avium subsp. paratuberculosis proteins that were upregulated under stress conditions
    Ratna B Gurung
    Faculty of Veterinary Science, University of Sydney, Camden, NSW, Australia
    Clin Vaccine Immunol 19:855-64. 2012
    ....
  34. ncbi Experimental animal infection models for Johne's disease, an infectious enteropathy caused by Mycobacterium avium subsp. paratuberculosis
    Douglas J Begg
    Farm Animal and Veterinary Public Health, Faculty of Veterinary Science, University of Sydney, Australia
    Vet J 176:129-45. 2008
    ....
  35. ncbi Evidence for age susceptibility of cattle to Johne's disease
    Peter A Windsor
    Faculty of Veterinary Science, University of Sydney, Camden, NSW 2570, Australia
    Vet J 184:37-44. 2010
    ..However the studies did show that if exposure to infection occurs at birth, then the risk of infection progressing to BJD is high, particularly in a highly contaminated environment or if the dam is infected...
  36. ncbi Culture of Mycobacterium avium subspecies paratuberculosis (MAP) from blood and extra-intestinal tissues in experimentally infected sheep
    Katrina L Bower
    Farm Animal and Veterinary Public Health, Faculty of Veterinary Science, University of Sydney, 425 Werombi Rd, Camden, NSW 2570, Australia
    Vet Microbiol 147:127-32. 2011
    ..Prolonged incubation periods prior to growth in BACTEC were consistent with inhibition of growth or dormancy in some blood cultures...
  37. ncbi Toll-like receptor (TLR)6 and TLR1 differentiation in gene expression studies of Johne's disease
    Karren M Plain
    Farm Animal and Veterinary Public Health, Faculty of Veterinary Science, University of Sydney, Camden 2570, Australia
    Vet Immunol Immunopathol 137:142-8. 2010
    ..A likely role for TLR6/TLR2 heterodimers in the pathogenesis of JD was identified. TLR6 may be a potential marker of exposure and could aid in the development of a gene signature for sheep resistant to MAP infection...
  38. ncbi Risk factors for ovine Johne's disease in infected sheep flocks in Australia
    Navneet K Dhand
    Faculty of Veterinary Science, The University of Sydney, 425 Werombi Road, Camden, NSW 2570, Australia
    Prev Vet Med 82:51-71. 2007
    ..Of the confounders investigated, infection was higher in flocks experiencing high mortalities; in wethers compared to ewes; and in 3-year-old sheep compared to 4-year-old sheep...
  39. ncbi Experimental infection of Australian freshwater fish with epizootic haematopoietic necrosis virus (EHNV)
    Joy A Becker
    University of Sydney, Camden, New South Wales, Australia
    J Aquat Anim Health 25:66-76. 2013
    ..Overall, it appeared that EHNV was less virulent in the present experimental model than in previous studies, but the reasons for this were not identified. Received May 21, 2012; accepted November 1, 2012...
  40. ncbi Culture phenotypes of genomically and geographically diverse Mycobacterium avium subsp. paratuberculosis isolates from different hosts
    Richard J Whittington
    Faculty of Veterinary Science, The University of Sydney, Camden, NSW, Australia
    J Clin Microbiol 49:1822-30. 2011
    ..avium subsp. paratuberculosis was observed. This optimal medium is recommended to remove bias in the isolation and cultivation of M. avium subsp. paratuberculosis...
  41. ncbi Modulation of inter-vaccination interval to avoid antigenic competition in multivalent footrot (Dichelobacter nodosus) vaccines in sheep
    Om P Dhungyel
    Faculty of Veterinary Science, The University of Sydney, Camden NSW 2570, Australia
    Vaccine 28:470-3. 2009
    ..nodosus. These results could have wider applications in vaccination against diseases caused by multivalent or multistrain microbes...
  42. ncbi Adsorption of Mycobacterium avium subsp. paratuberculosis to soil particles
    Navneet K Dhand
    Faculty of Veterinary Science, The University of Sydney, Camden, New South Wales 2570, Australia
    Appl Environ Microbiol 75:5581-5. 2009
    ..paratuberculosis to soil particles at an acidic pH (P < 0.05). The results suggest that under experimental conditions M. avium subsp. paratuberculosis adsorbs to a range of soil particles, and this attachment is influenced by soil pH...
  43. ncbi Partial proteome of Mycobacterium avium subsp. paratuberculosis under oxidative and nitrosative stress
    Satoko Kawaji
    Faculty of Veterinary Science, The University of Sydney, 425 Werombi Road, Camden, New South Wales 2570, Australia
    Vet Microbiol 145:252-64. 2010
    ....
  44. ncbi In silico screened Mycobacterium avium subsp. paratuberculosis (MAP) recombinant proteins upregulated under stress conditions are immunogenic in sheep
    Ratna B Gurung
    Faculty of Veterinary Science, University of Sydney, Camden, NSW, Australia
    Vet Immunol Immunopathol 149:186-96. 2012
    ..At 91% diagnostic specificity, the diagnostic sensitivity of the recombinant antigen ELISA ranged from 24% to 42% and AUC(ROC) from 0.7015 to 0.8405...
  45. ncbi Validation of endogenous reference genes for expression profiling of RAW264.7 cells infected with Mycobacterium avium subsp. paratuberculosis by quantitative PCR
    Deborah L Taylor
    Faculty of Veterinary Science, J L Shute Building, University of Sydney, Camden, NSW 2570, Australia
    Vet Immunol Immunopathol 115:43-55. 2007
    ....
  46. ncbi Comparative study of the commonly used virulence tests for laboratory diagnosis of ovine footrot caused by Dichelobacter nodosus in Australia
    Om P Dhungyel
    Farm Animal and Veterinary Public Health, Faculty of Veterinary Science, The University of Sydney, 425 Werombi Rd, Camden, NSW 2570, Australia
    Vet Microbiol 162:756-60. 2013
    ..Only about 21% of the isolates were virulent in all 3 tests. Therefore these tests on their own may not provide standard and reliable results and are likely to remain as supplementary tests for clinical diagnosis of the disease...
  47. ncbi Tissue localisation of Mycobacterium avium subspecies paratuberculosis following artificially induced intracellular and naked bacteraemia
    Katrina L Bower
    Farm Animal and Veterinary Public Health, Faculty of Veterinary Science, University of Sydney, 425 Werombi Rd, Camden, NSW 2570, Australia
    Vet Microbiol 162:112-8. 2013
    ..The rapidity of localisation of MAP to tissues after onset of induced bacteraemia suggests that intermittent seeding into blood in natural infection would be sufficient to account for dissemination...
  48. ncbi Protein extraction from Mycobacterium avium subsp. paratuberculosis: Comparison of methods for analysis by sodium dodecyl sulphate polyacrylamide gel electrophoresis, native PAGE and surface enhanced laser desorption/ionization time of flight mass spectro
    Sanjeev Gumber
    Faculty of Veterinary Science, The University of Sydney, Private Bag 3, Camden 2570, NSW Australia
    J Microbiol Methods 68:115-27. 2007
    ..All buffers were suitable for protein extraction for SDS-PAGE, while Tween20 was best for native PAGE...
  49. ncbi An innovative approach to post-graduate education in veterinary public health
    Jenny Ann L M L Toribio
    Faculty of Veterinary Science C01, Camden Campus, The University of Sydney, Australia
    J Vet Med Educ 36:114-21. 2009
    ....
  50. ncbi Optimisation and validation of a real-time reverse transcriptase-polymerase chain reaction assay for detection of betanodavirus
    Paul Hick
    Farm Animal and Veterinary Public Health, Faculty of Veterinary Science, University of Sydney, 425 Werombi Road Camden, NSW 2570, Australia
    J Virol Methods 163:368-77. 2010
    ..This is the first study to report the diagnostic performance of a RT-qPCR assay for detection of betanodavirus...