David M Whiley

Summary

Affiliation: University of Queensland
Country: Australia

Publications

  1. ncbi request reprint Detection of Neisseria Meningitidis in clinical samples by a duplex real-time PCR targeting the porA and ctrA genes
    David M Whiley
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Herston, and Clinical Medical Virology Centre, University of Queensland, Brisbane, Queensland, Australia
    Mol Diagn 7:141-5. 2003
  2. pmc A comparison of two informative SNP-based strategies for typing Pseudomonas aeruginosa isolates from patients with cystic fibrosis
    Melanie W Syrmis
    Queensland Children s Medical Research Institute, The University of Queensland, Brisbane, Queensland 4029, Australia
    BMC Infect Dis 14:307. 2014
  3. doi request reprint Real-time PCR genotyping of Neisseria gonorrhoeae isolates using 14 informative single nucleotide polymorphisms on gonococcal housekeeping genes
    David M Whiley
    Queensland Paediatric Infectious Diseases Laboratory, Queensland Children s Medical Research Institute, Children s Health Service District, Queensland, Australia
    J Antimicrob Chemother 68:322-8. 2013
  4. doi request reprint The ticking time bomb: escalating antibiotic resistance in Neisseria gonorrhoeae is a public health disaster in waiting
    David M Whiley
    Queensland Paediatric Infectious Diseases Laboratory, Queensland Children s Medical Research Institute, Children s Health Service, Brisbane, Queensland, Australia
    J Antimicrob Chemother 67:2059-61. 2012
  5. doi request reprint Improved detection of genetic markers of antimicrobial resistance by hybridization probe-based melting curve analysis using primers to mask proximal mutations: examples include the influenza H275Y substitution
    David M Whiley
    Queensland Paediatric Infectious Diseases Laboratory, Queensland Children s Medical Research Institute, Children s Health Service District, Brisbane, Queensland, Australia
    J Antimicrob Chemother 67:1375-9. 2012
  6. pmc Viral-bacterial co-infection in Australian Indigenous children with acute otitis media
    Michael J Binks
    Ear and Respiratory Unit, Child Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, Australia
    BMC Infect Dis 11:161. 2011
  7. ncbi request reprint Sequence variation can affect the performance of minor groove binder TaqMan probes in viral diagnostic assays
    David M Whiley
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Herston Road, Herston, QLD 4029, Australia
    J Clin Virol 35:81-3. 2006
  8. ncbi request reprint A 5'-nuclease real-time reverse transcriptase-polymerase chain reaction assay for the detection of a broad range of influenza A subtypes, including H5N1
    David M Whiley
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Queensland, 4029 Australia
    Diagn Microbiol Infect Dis 53:335-7. 2005
  9. pmc Nucleic acid amplification testing for Neisseria gonorrhoeae: an ongoing challenge
    David M Whiley
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Herston Road, Herston, Queensland, Australia 4029
    J Mol Diagn 8:3-15. 2006
  10. ncbi request reprint False-negative results in nucleic acid amplification tests-do we need to routinely use two genetic targets in all assays to overcome problems caused by sequence variation?
    D M Whiley
    Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service Distric, Queensland, Australia
    Crit Rev Microbiol 34:71-6. 2008

Collaborators

Detail Information

Publications67

  1. ncbi request reprint Detection of Neisseria Meningitidis in clinical samples by a duplex real-time PCR targeting the porA and ctrA genes
    David M Whiley
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Herston, and Clinical Medical Virology Centre, University of Queensland, Brisbane, Queensland, Australia
    Mol Diagn 7:141-5. 2003
    ..In recent years PCR has proven to be a highly sensitive and specific method for the diagnosis of infections caused by Neisseria meningitidis...
  2. pmc A comparison of two informative SNP-based strategies for typing Pseudomonas aeruginosa isolates from patients with cystic fibrosis
    Melanie W Syrmis
    Queensland Children s Medical Research Institute, The University of Queensland, Brisbane, Queensland 4029, Australia
    BMC Infect Dis 14:307. 2014
    ..aeruginosa SNPs and using real-time polymerase chain reaction technology (HRM10SNP) and the other targeting 20 SNPs and based on the Sequenom MassARRAY platform (iPLEX20SNP)...
  3. doi request reprint Real-time PCR genotyping of Neisseria gonorrhoeae isolates using 14 informative single nucleotide polymorphisms on gonococcal housekeeping genes
    David M Whiley
    Queensland Paediatric Infectious Diseases Laboratory, Queensland Children s Medical Research Institute, Children s Health Service District, Queensland, Australia
    J Antimicrob Chemother 68:322-8. 2013
    ..In this study, we investigated single nucleotide polymorphism (SNP)-based profiling as a means of circumventing these problems...
  4. doi request reprint The ticking time bomb: escalating antibiotic resistance in Neisseria gonorrhoeae is a public health disaster in waiting
    David M Whiley
    Queensland Paediatric Infectious Diseases Laboratory, Queensland Children s Medical Research Institute, Children s Health Service, Brisbane, Queensland, Australia
    J Antimicrob Chemother 67:2059-61. 2012
    ..Unless there is urgent action at international and local levels to combat the problem of N. gonorrhoeae antimicrobial resistance, we are in for gloomy times ahead in terms of gonorrhoea disease and control...
  5. doi request reprint Improved detection of genetic markers of antimicrobial resistance by hybridization probe-based melting curve analysis using primers to mask proximal mutations: examples include the influenza H275Y substitution
    David M Whiley
    Queensland Paediatric Infectious Diseases Laboratory, Queensland Children s Medical Research Institute, Children s Health Service District, Brisbane, Queensland, Australia
    J Antimicrob Chemother 67:1375-9. 2012
    ..However, the performance of these methods can be undermined by sequence variation in the regions flanking the codon of interest. This is a problem encountered more broadly in microbial diagnostics...
  6. pmc Viral-bacterial co-infection in Australian Indigenous children with acute otitis media
    Michael J Binks
    Ear and Respiratory Unit, Child Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, Australia
    BMC Infect Dis 11:161. 2011
    ..Our hypothesis is that antecedent respiratory viral infection increases bacterial density and progression to perforation...
  7. ncbi request reprint Sequence variation can affect the performance of minor groove binder TaqMan probes in viral diagnostic assays
    David M Whiley
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Herston Road, Herston, QLD 4029, Australia
    J Clin Virol 35:81-3. 2006
    ..Overall, these results highlight the usefulness of MGB TaqMan probes for the detection of mismatches, but suggest that MGB Taqman probes have limitations for routine screening for uncharacterised viral strains...
  8. ncbi request reprint A 5'-nuclease real-time reverse transcriptase-polymerase chain reaction assay for the detection of a broad range of influenza A subtypes, including H5N1
    David M Whiley
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Queensland, 4029 Australia
    Diagn Microbiol Infect Dis 53:335-7. 2005
    ..The results show the assay is suitable for screening for influenza A infections, particularly in regions where avian strains may be circulating...
  9. pmc Nucleic acid amplification testing for Neisseria gonorrhoeae: an ongoing challenge
    David M Whiley
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Herston Road, Herston, Queensland, Australia 4029
    J Mol Diagn 8:3-15. 2006
    ..The need to evaluate N. gonorrhoeae NAATs before their use in any new patient population and to educate physicians on the limitations of these tests is emphasized in this review...
  10. ncbi request reprint False-negative results in nucleic acid amplification tests-do we need to routinely use two genetic targets in all assays to overcome problems caused by sequence variation?
    D M Whiley
    Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service Distric, Queensland, Australia
    Crit Rev Microbiol 34:71-6. 2008
    ..In light of these ongoing problems, it may be time to consider the use of two genetic targets in NAAT methods to reduce the potential for sequence-related false-negative results...
  11. doi request reprint Reduced susceptibility to ceftriaxone in Neisseria gonorrhoeae is associated with mutations G542S, P551S and P551L in the gonococcal penicillin-binding protein 2
    David M Whiley
    Queensland Paediatric Infectious Diseases Laboratory, Queensland Children s Medical Research Institute, Children s Health Service District, Queensland, Australia
    J Antimicrob Chemother 65:1615-8. 2010
    ..In this study, we examined an association between reduced susceptibility to ceftriaxone and additional mutations in gonococcal PBP2...
  12. doi request reprint Neisseria gonorrhoeae multi-antigen sequence typing using non-cultured clinical specimens
    David M Whiley
    Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Herston Road, Herston, Queensland 4029, Australia
    Sex Transm Infect 86:51-5. 2010
    ..This study sought to examine the performance of the NG-MAST system on a range of non-cultured samples...
  13. ncbi request reprint A simple approach for preparing real-time PCR positive reaction controls for rare or emerging viruses
    David M Whiley
    Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Queensland Children s Medical Research Institute, Children s Health Service District, Queensland, Australia
    J Clin Virol 48:193-7. 2010
    ..This is particularly problematic during outbreaks caused by emerging infectious diseases, when delays can impede the public health response...
  14. ncbi request reprint Detection of novel influenza A(H1N1) virus by real-time RT-PCR
    David M Whiley
    Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Queensland Children s Medical Research Institute, Children s Health Service District, Queensland, Australia
    J Clin Virol 45:203-4. 2009
    ..Overall, the results showed that the RT-PCR methods were suitable for sensitive and specific detection of novel influenza A(H1N1) RNA in human samples...
  15. ncbi request reprint Evidence that the gonococcal porA pseudogene is present in a broad range of Neisseria gonorrhoeae strains; suitability as a diagnostic target
    David M Whiley
    Microbiology Unit, Canterbury Health Laboratories, Christchurch, New Zealand
    Pathology 38:445-8. 2006
    ..The primary aim of the study was to determine if the gonococcal porA pseudogene is a stable sequence target for the detection of Neisseria gonorrhoeae by PCR...
  16. pmc Detection of novel polyomaviruses, TSPyV, HPyV6, HPyV7, HPyV9 and MWPyV in feces, urine, blood, respiratory swabs and cerebrospinal fluid
    Rebecca J Rockett
    Queensland Paediatric Infectious Diseases Laboratory, Queensland Children s Medical Research Institute, The University of Queensland, Brisbane, Queensland, Australia
    PLoS ONE 8:e62764. 2013
    ..The other novel polyomaviruses were also found in respiratory and fecal specimens, but at lower prevalence and most commonly in immunocompromised individuals...
  17. doi request reprint Evaluation of the cobas 4800 CT/NG test for detecting Chlamydia trachomatis and Neisseria gonorrhoeae
    Rebecca Rockett
    Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Queensland Children s Medical Research Institute, Children s Health Service District, Queensland, Australia
    Sex Transm Infect 86:470-3. 2010
    ..To investigate the performance of the fully automated cobas 4800 CT/NG test for detection of Chlamydia trachomatis and Neisseria gonorrhoeae...
  18. doi request reprint The influence of target population on nonculture-based detection of markers of Neisseria gonorrhoeae antimicrobial resistance
    Namraj Goire
    Queensland Paediatric Infectious Diseases Laboratory, Queensland Children s Medical Research Institute, Brisbane, Australia
    Sex Health 9:422-9. 2012
    ..With treatment options for gonorrhoea (Neisseria gonorrhoeae) diminishing, strengthening antimicrobial resistance (AMR) surveillance is paramount...
  19. doi request reprint Enhanced gonococcal antimicrobial surveillance in the era of ceftriaxone resistance: a real-time PCR assay for direct detection of the Neisseria gonorrhoeae H041 strain
    Namraj Goire
    Queensland Paediatric Infectious Diseases Laboratory, Queensland Children s Medical Research Institute, Children s Health Service District, Queensland, Australia
    J Antimicrob Chemother 67:902-5. 2012
    ..In this study we developed a real-time PCR assay for direct detection of the H041 strain...
  20. pmc Diversity of penA alterations and subtypes in Neisseria gonorrhoeae strains from Sydney, Australia, that are less susceptible to ceftriaxone
    David M Whiley
    Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Brisbane, Queensland, Australia
    Antimicrob Agents Chemother 51:3111-6. 2007
    ..Overall, the results of our study show that N. gonorrhoeae strains exhibiting reduced sensitivity to ceftriaxone are not of a particular subtype and that a number of different mutations in PBP 2 may contribute to this phenomenon...
  21. ncbi request reprint Exploring 'best practice' for nucleic acid detection of Neisseria gonorrhoeae
    David M Whiley
    Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Brisbane, QLD 4029, Australia
    Sex Health 5:17-23. 2008
    ..Further, we highlight the need to maintain culture-based testing for certain specimen sites as well as for antimicrobial resistance surveillance...
  22. pmc Merkel cell polyomavirus DNA in respiratory specimens from children and adults
    Seweryn Bialasiewicz
    Research Section, Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Brisbane, Queensland, Australia
    Emerg Infect Dis 15:492-4. 2009
    ..Partial T antigen and major capsid protein sequences of MCPyV identified in respiratory secretions showed high homology (99%-100%) to those found in Merkel cell carcinoma...
  23. doi request reprint A duplex Neisseria gonorrhoeae real-time polymerase chain reaction assay targeting the gonococcal porA pseudogene and multicopy opa genes
    Namraj Goire
    Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Queensland 4029, Australia
    Diagn Microbiol Infect Dis 61:6-12. 2008
    ..In addition, the 2-target system of the NGduplex assay decreases the potential for sequence-related false-negative results and can provide simultaneous confirmation of positive results...
  24. pmc Enhancing gonococcal antimicrobial resistance surveillance: a real-time PCR assay for detection of penicillinase-producing Neisseria gonorrhoeae by use of noncultured clinical samples
    Namraj Goire
    Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Herston Road, Herston, Queensland 4029, Australia
    J Clin Microbiol 49:513-8. 2011
    ..gonorrhoeae penicillin resistance and is of particular relevance to regions where penicillin is still used to treat gonorrhea...
  25. ncbi request reprint Detection of BK, JC, WU, or KI polyomaviruses in faecal, urine, blood, cerebrospinal fluid and respiratory samples
    Seweryn Bialasiewicz
    Queensland Pediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, QLD, Australia
    J Clin Virol 45:249-54. 2009
    ..The related human polyomaviruses JCV and BKV in contrast, have been detected in a wide range of sample types, leading to increased knowledge about their biology and pathogenesis...
  26. pmc Simple, rapid, and inexpensive detection of Neisseria gonorrhoeae resistance mechanisms using heat-denatured isolates and SYBR green-based real-time PCR
    Gayle Kugelman
    Queensland Children s Medical Research Institute, Infectious Diseases Laboratory, Royal Children s Hospital, Herston Road, Herston, QLD 4029, Queensland Australia
    Antimicrob Agents Chemother 53:4211-6. 2009
    ..These methods may have broader application in the detection of polymorphisms associated with phenotypes of interest...
  27. ncbi request reprint Screening for H7N9 influenza A by matrix gene-based real-time reverse-transcription PCR
    Hazel Hackett
    Queensland Paediatric Infectious Diseases Laboratory, Royal Children s Hospital, Brisbane, Queensland, Australia Queensland Children s Medical Research Institute, Royal Children s Hospital, The University of Queensland, Brisbane, Queensland, Australia
    J Virol Methods 195:123-5. 2014
    ..However, cross-reactions were observed with H5N3, H9N2 and H7N7. Overall, the results show that the methods are useful for front-line screening for H7N9. ..
  28. doi request reprint Molecular approaches to enhance surveillance of gonococcal antimicrobial resistance
    Namraj Goire
    Queensland Paediatric Infectious Diseases Laboratory and Queensland Children s Medical Research Institute, Royal Children s Hospital, The University of Queensland, Brisbane, Queensland 4029, Australia
    Nat Rev Microbiol 12:223-9. 2014
    ..gonorrhoeae AMR and propose the use of molecular methods on a large scale to systematically enhance surveillance. ..
  29. ncbi request reprint Simultaneous detection and differentiation of human polyomaviruses JC and BK by a rapid and sensitive PCR-ELAHA assay and a survey of the JCV subtypes within an Australian population
    David M Whiley
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Queensland, Australia
    J Med Virol 72:467-72. 2004
    ..The results of this study show that the VP1-PCR-ELAHA is a sensitive, specific and rapid method for detecting and differentiating human polyomaviruses JC and BK and is highly suitable for routine use in the clinical laboratory...
  30. doi request reprint Comparing nose-throat swabs and nasopharyngeal aspirates collected from children with symptoms for respiratory virus identification using real-time polymerase chain reaction
    Stephen B Lambert
    MBBS, Queensland Paediatric Infectious Diseases Laboratory, Royal Children s Hospital, Herston Queensland 4029, Australia
    Pediatrics 122:e615-20. 2008
    ..The objective of this study was to calculate sensitivity values for the detection of major respiratory viruses of childhood by using combined nose-throat swabs and nasopharyngeal aspirates...
  31. pmc A sensitive, specific, and cost-effective multiplex reverse transcriptase-PCR assay for the detection of seven common respiratory viruses in respiratory samples
    Melanie W Syrmis
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Queensland, Australia
    J Mol Diagn 6:125-31. 2004
    ....
  32. pmc Molecular assays for detection of human metapneumovirus
    Ian M Mackay
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Brisbane, Queensland, Australia
    J Clin Microbiol 41:100-5. 2003
    ..6%) tested during the comparison of the two diagnostic approaches. We found the real-time RT-PCR to be the test of choice for future investigation of samples for hMPV due to its speed, reproducibility, specificity, and sensitivity...
  33. doi request reprint High-throughput single-nucleotide polymorphism-based typing of shared Pseudomonas aeruginosa strains in cystic fibrosis patients using the Sequenom iPLEX platform
    Melanie W Syrmis
    Queensland Paediatric Infectious Diseases Laboratory, Royal Children s Hospital, Brisbane, Queensland, Australia
    J Med Microbiol 62:734-40. 2013
    ..SNP results for the 56 non-CF isolates were consistent with previous MLST data. Thus, the PA iPLEX format provides an attractive high-throughput alternative to ERIC-PCR for large-scale investigations of shared P. aeruginosa strains...
  34. ncbi request reprint Detection and differentiation of Plasmodium species by polymerase chain reaction and colorimetric detection in blood samples of patients with suspected malaria
    David M Whiley
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Clinical Medical Virology Centre, University of Queensland, Brisbane, Queensland, Australia
    Diagn Microbiol Infect Dis 49:25-9. 2004
    ..In summary, the sensitivity, specificity and simplicity of the PCR assay makes it particularly suitable for use in a diagnostic laboratory...
  35. doi request reprint Detection of human bocavirus in respiratory, fecal, and blood samples by real-time PCR
    Sarah J Tozer
    Queensland Pediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Herston Road, Herston, Queensland, Australia
    J Med Virol 81:488-93. 2009
    ..HBoV was found in three different specimen types: parent-collected combined nose-throat swabs, fecal samples collected from symptomatic individuals and whole blood from immunocompromised children...
  36. pmc Detection of human respiratory syncytial virus in respiratory samples by LightCycler reverse transcriptase PCR
    David M Whiley
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Herston, Queensland, Australia
    J Clin Microbiol 40:4418-22. 2002
    ..The sensitivity of LC-RT-PCR was 50 PFU/ml; and this, together with its high specificity and rapid turnaround time, makes the LC-RT-PCR suitable for the detection of hRSV in clinical specimens...
  37. doi request reprint Successful application of a simple specimen transport method for the conduct of respiratory virus surveillance in remote Indigenous communities in Australia
    Kerry Ann F O'Grady
    Queensland Children s Medical Research Institute, Herston, QLD, Australia
    Trop Med Int Health 16:766-72. 2011
    ..This study assessed the sensitivity of a simple method for transporting respiratory samples from a remote setting for viral PCR compared with frozen specimens...
  38. pmc A Novel Duplex Real-Time Reverse-Transcription PCR Assay for the Detection of Influenza A and the Novel Influenza A(H1N1) Strain
    Rebecca J Rockett
    Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Queensland Children s Medical Research Institute, Children s Health Service District, Queensland, Australia E Mails S B D M W C E F S B L M D N T P S
    Viruses 1:1204-8. 2009
    ..Overall, the results showed that the dFLU-TM RT-PCR method is suitable for detection of influenza A, including the novel H1N1 pandemic strain, in clinical samples...
  39. ncbi request reprint High-throughput informative single nucleotide polymorphism-based typing of Neisseria gonorrhoeae using the Sequenom MassARRAY iPLEX platform
    Ella Trembizki
    Queensland Paediatric Infectious Diseases Laboratory, Royal Children s Hospital, Brisbane, Queensland 4029, Australia Queensland Children s Medical Research Institute, Royal Children s Hospital, The University of Queensland, Brisbane, Queensland 4029, Australia
    J Antimicrob Chemother 69:1526-32. 2014
    ..gonorrhoeae AMR. We report on the development, validation and testing of a Sequenom MassARRAY iPLEX method for multilocus sequence typing (MLST)-style genotyping of N. gonorrhoeae isolates...
  40. ncbi request reprint Co-detection and discrimination of six human herpesviruses by multiplex PCR-ELAHA
    Ian M Mackay
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Brisbane, Australia
    J Clin Virol 28:291-302. 2003
    ..Multiplex PCR (mPCR) is capable of simultaneously amplifying a range of targets from a single preparation of nucleic acids and when combined with a suitable detection assay, it is capable of discriminating each of the amplicons...
  41. doi request reprint Alterations of the pilQ gene in Neisseria gonorrhoeae are unlikely contributors to decreased susceptibility to ceftriaxone and cefixime in clinical gonococcal strains
    David M Whiley
    Queensland Children s Medical Research Institute, Children s Health Service District, Brisbane, Queensland, Australia
    J Antimicrob Chemother 65:2543-7. 2010
    ..In this study, we investigated whether pilQ polymorphisms were associated with decreased susceptibility to extended-spectrum cephalosporins (ESCs) in clinical gonococcal strains...
  42. ncbi request reprint A newly reported human polyomavirus, KI virus, is present in the respiratory tract of Australian children
    Seweryn Bialasiewicz
    Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Brisbane, Queensland, Australia
    J Clin Virol 40:15-8. 2007
    ..Recently, Allander and co-workers reported the discovery of a new human polyomavirus, KI virus, in respiratory secretions from patients with acute respiratory tract infection (ARTI)...
  43. ncbi request reprint A real-time PCR assay for the detection of Neisseria gonorrhoeae in genital and extragenital specimens
    David M Whiley
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Queensland 4029, Australia
    Diagn Microbiol Infect Dis 52:1-5. 2005
    ..For the bacterial panel, only N. gonorrhoeae isolates provided positive results. The results show the NGpapLC assay is suitable for use on a range of clinical specimens and could improve detection of pharyngeal N. gonorrhoeae...
  44. doi request reprint Protocol for the use of enzyme-linked hybridization assays for genital ulcer disease
    Seweryn Bialasiewicz
    Sir Albert Sakzewski Virus Research Centre, Herston, QLD, Australia
    Methods Mol Biol 903:225-33. 2012
    ..In this protocol, we describe the simultaneous detection of these five pathogens and an internal control using a single-tube multiplex PCR and colorimetric enzyme-linked amplicon hybridization assay...
  45. pmc A real-time, quantitative PCR method using hydrolysis probes for the monitoring of Plasmodium falciparum load in experimentally infected human volunteers
    Rebecca J Rockett
    Queensland Paediatric Infectious Diseases Laboratory, Queensland Children s Medical Research Institute, Queensland Children s Health Service and The University of Queensland, Queensland, Australia
    Malar J 10:48. 2011
    ..The accurate quantification of Plasmodium falciparum parasite numbers by PCR is an important tool for monitoring growth kinetics in subjects infected and subsequently treated with anti-malarial agents...
  46. ncbi request reprint Detection and differentiation of herpes simplex virus types 1 and 2 by a duplex LightCycler PCR that incorporates an internal control PCR reaction
    David M Whiley
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Herston Road, Herston, Queensland 4029, Australia
    J Clin Virol 30:32-8. 2004
    ..The advent of real-time HSV PCR protocols now enables rapid result turnaround times with minimal hands-on time...
  47. pmc Impact of competitive inhibition and sequence variation upon the sensitivity of malaria PCR
    Seweryn Bialasiewicz
    Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Building C28, Back Road, Royal Children s Hospital and Health Service District, Herston, Queensland, Australia 4029
    J Clin Microbiol 45:1621-3. 2007
    ..Sequence variation in oligomer targets and competitive inhibition of dual-species templates in universal-primer mixes were found to decrease assay sensitivity...
  48. ncbi request reprint Comparison of three in-house multiplex PCR assays for the detection of Neisseria gonorrhoeae and Chlamydia trachomatis using real-time and conventional detection methodologies
    David M Whiley
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Brisbane, Queensland, Australia
    Pathology 37:364-70. 2005
    ..To develop and evaluate multiplex PCR assays for Chlamydia trachomatis and Neisseria gonorrhoeae, using real-time and conventional PCR detection methodologies...
  49. pmc Nasal swab samples and real-time polymerase chain reaction assays in community-based, longitudinal studies of respiratory viruses: the importance of sample integrity and quality control
    Asma N Alsaleh
    Queensland Children s Medical Research Institute, The University of Queensland, Brisbane Queensland 4029, Australia
    BMC Infect Dis 14:15. 2014
    ..We therefore investigated the impact of sample collection quality and the presence of visible mould in samples upon respiratory virus detection by real-time polymerase chain reaction (PCR) assays...
  50. ncbi request reprint Sequence variation in primer targets affects the accuracy of viral quantitative PCR
    David M Whiley
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Herston, QLD 4029, Australia
    J Clin Virol 34:104-7. 2005
    ..When using these primers in standard Taqman two-step cycling conditions, as few as two mismatches in a single primer increased cycle threshold values and significantly influenced the calculation of viral load...
  51. pmc Mailed versus frozen transport of nasal swabs for surveillance of respiratory bacteria in remote Indigenous communities in Australia
    Kerry Ann F O'Grady
    Queensland Children s Medical Research Institute, Queensland University of Technology, Herston Road HERSTON QLD, 4029 Herston, Australia
    BMC Infect Dis 13:543. 2013
    ..This study assessed the sensitivity of a simple method for transporting nasal swabs from a remote setting for bacterial polymerase chain reaction (PCR) testing...
  52. doi request reprint Direct urine polymerase chain reaction for chlamydia and gonorrhoea: a simple means of bringing high-throughput rapid testing to remote settings?
    Frashta Rahimi
    Queensland Paediatric Infectious Diseases Laboratory, Queensland Children s Medical Research Institute, Children s Health Service District, Brisbane, QLD 4029, Australia
    Sex Health 10:299-304. 2013
    ..gonorrhoeae-positive samples. Conclusions: The results of this study show that the simplified PCR strategy may be a feasible approach for rapid screening and improving chlamydia and gonorrhoea treatment in remote settings. ..
  53. doi request reprint Protocol for the molecular detection of antibiotic resistance mechanisms in Neisseria gonorrhoeae
    Namraj Goire
    Queensland Paediatric Infectious Diseases Laboratory, Queensland Children s Medical Research Institute, Sir Albert Sakzewski Virus Research Centre, Children s Health Services, The University of Queensland, Herston, Brisbane, QLD, Australia
    Methods Mol Biol 903:319-28. 2012
    ..In this chapter, we discuss nucleic acid amplification-based detection of AMR in gonorrhoea with a particular emphasis on chromosomal-mediated resistance to beta-lactam antibiotics...
  54. ncbi request reprint A real-time PCR assay for the detection of Neisseria gonorrhoeae by LightCycler
    David M Whiley
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Brisbane, Queensland, Australia
    Diagn Microbiol Infect Dis 42:85-9. 2002
    ..These features combined with the rapid turn-around time for results makes the LC-PCR particularly suitable for the detection of N. gonorrhoeae in a routine clinical laboratory...
  55. doi request reprint A novel gel-based method for self-collection and ambient temperature postal transport of urine for PCR detection of Chlamydia trachomatis
    S Bialasiewicz
    Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, University of Queensland, Clinical Medical Virology Centre, Queensland, Australia
    Sex Transm Infect 85:102-5. 2009
    ..The method needed to be suitable for C trachomatis PCR detection, be economical and suitable for transport by standard envelope mailing...
  56. ncbi request reprint Emerging respiratory agents: new viruses for old diseases?
    T P Sloots
    Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Queensland, Australia
    J Clin Virol 42:233-43. 2008
    ..We review the new viral agents that have been detected in respiratory samples since 2001, and examine their contribution as agents of human disease...
  57. doi request reprint A retrospective performance evaluation of an adenovirus real-time PCR assay
    Asma N Alsaleh
    Queensland Children s Medical Research Institute, Royal Children s Hospital, The University of Queensland, Brisbane, Queensland, Australia Queensland Paediatric Infectious Disease Laboratory, Royal Children s Hospital, Brisbane, Queensland, Australia Department of Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia
    J Med Virol 86:795-801. 2014
    ..Reassuringly, the results show that despite the high level of sequence variation in the AdV-PCR assay oligonucleotide targets, the assay remains suitable for routine detection of human AdV strains...
  58. ncbi request reprint A new confirmatory Neisseria gonorrhoeae real-time PCR assay targeting the porA pseudogene
    D M Whiley
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Clinical Medical Virology Centre, University of Queensland, Herston, Brisbane, Australia
    Eur J Clin Microbiol Infect Dis 23:705-10. 2004
    ..gonorrhoeae DNA. The results of this study show the NGpapLC assay is a suitable alternative to the cppB-LC assay for confirmation of N. gonorrhoeae-positive results obtained with Cobas Amplicor...
  59. ncbi request reprint Preliminary comparison of three LightCycler PCR assays for the detection of herpes simplex virus in swab specimens
    D M Whiley
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Herston Road, 4029 Herston, Queensland, Australia
    Eur J Clin Microbiol Infect Dis 22:764-7. 2003
    ..However, differentiation of herpes simplex virus types 1 and 2 by melting curve analysis was problematic in all assays. Overall, the results highlight the limitations of typing herpes simplex virus by melting curve analysis...
  60. pmc Detection and differentiation of human polyomaviruses JC and BK by LightCycler PCR
    D M Whiley
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital and Health Service District, Queensland, Australia
    J Clin Microbiol 39:4357-61. 2001
    ..These data, combined with its rapid turnaround time for results and decreased hands-on time, make the LightCycler PCR assay highly suitable for the rapid detection and differentiation of JCV and BKV in the clinical laboratory...
  61. ncbi request reprint Presence of the newly discovered human polyomaviruses KI and WU in Australian patients with acute respiratory tract infection
    S Bialasiewicz
    Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Herston Road, Herston, Queensland 4029, Australia
    J Clin Virol 41:63-8. 2008
    ..Currently, the role of the novel human polyomaviruses, KI (KIV) and WU (WUV) as agents of human disease remains uncertain...
  62. ncbi request reprint Low positive predictive value of a nucleic acid amplification test for nongenital Neisseria gonorrhoeae infection in homosexual men
    Leon P McNally
    Molecular Diagnostic Medicine Laboratory, SydPath, St Vincent s Hospital, Darlinghurst, NSW
    Clin Infect Dis 47:e25-7. 2008
    ..Only 30.4% of oropharyngeal samples and 73.7% of anorectal samples were positive for N. gonorrhoeae by the porA assay. The accuracy of nucleic acid amplification tests in this context is compromised without supplemental testing...
  63. doi request reprint Identification of Australian human respiratory syncytial virus strains containing a 60-nucleotide duplication within the G glycoprotein gene
    Sarah Tozer
    Pathology 40:632-5. 2008
  64. ncbi request reprint Detection of Neisseria meningitidis by LightCycler PCR
    David M Whiley
    Pathology 35:347-9. 2003
  65. pmc Identification of a novel polyomavirus from patients with acute respiratory tract infections
    Anne M Gaynor
    Department of Molecular Microbiology, Washington University School of Medicine, St Louis, Missouri, United States of America
    PLoS Pathog 3:e64. 2007
    ..The presence of multiple instances of the virus in two continents suggests that this virus is geographically widespread in the human population and raises the possibility that the WU virus may be a human pathogen...
  66. pmc Further questions regarding the role of mosaic penA sequences in conferring reduced susceptibility to ceftriaxone in Neisseria gonorrhoeae
    David M Whiley
    Antimicrob Agents Chemother 51:802-3. 2007
  67. ncbi request reprint Melting curve analysis using hybridisation probes: limitations in microbial molecular diagnostics
    David M Whiley
    Pathology 37:254-6. 2005