Research Topics
Genomes and Genes | Jill TrewhellaSummaryAffiliation: University of Sydney Country: Australia Publications
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Publications
Protein kinase A targeting and activation as seen by small-angle solution scatteringJill Trewhella
School of Molecular and Microbial Biosciences, University of Sydney, Sydney 2006, NSW, Australia
Eur J Cell Biol 85:655-62. 2006....- Structural themes and variations in protein kinase A as seen by small-angle scattering and neutron contrast variationJill Trewhella
School of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia
Eur Biophys J 35:585-9. 2006..These studies demonstrate the power of neutron contrast variation to expand our knowledge of the dynamic supra-molecular structures that carry out biological function... 
The different views from small anglesJill Trewhella
School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia
Proc Natl Acad Sci U S A 105:4967-8. 2008
Histidine kinase regulation by a cyclophilin-like inhibitorDavid A Jacques
School of Molecular and Microbial Biosciences, University of Sydney, Sydney, New South Wales 2006, Australia
J Mol Biol 384:422-35. 2008..Given that both KinA and KipI homologues are widespread in the bacterial kingdom, this mechanism has broad significance in bacterial signal transduction...- A chemically modified alpha-amylase with a molten-globule state has entropically driven enhanced thermal stabilityKhawar Sohail Siddiqui
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia
Protein Eng Des Sel 23:769-80. 2010..This study provides new insight into the intriguing properties of an MG state resulting from the chemical modification of TAA... - The C0C1 fragment of human cardiac myosin binding protein C has common binding determinants for both actin and myosinYanling Lu
School of Molecular Bioscience, University of Sydney, NSW 2006, Australia
J Mol Biol 413:908-13. 2011..Our results suggest that C0 and C1 interact with myosin and actin using a common set of binding determinants and therefore support a cMyBP-C switching mechanism between myosin and actin... - A novel structure of an antikinase and its inhibitorDavid A Jacques
School of Molecular Bioscience, University of Sydney, NSW 2006, Australia
J Mol Biol 405:214-26. 2011..We discuss the sequence conservation and possible roles across species of this near-ubiquitous protein family, which is poorly understood in terms of function... - Human cardiac myosin binding protein C: structural flexibility within an extended modular architectureCy M Jeffries
School of Molecular Bioscience, University of Sydney, New South Wales 2006, Australia
J Mol Biol 414:735-48. 2011..These structural features of cMyBP-C could facilitate its putative role as a molecular switch between actin and myosin and may contribute to modulating the transverse pliancy of the C-zone of the A-band across muscle sarcomeres... - The structure of TTHA0988 from Thermus thermophilus, a KipI-KipA homologue incorrectly annotated as an allophanate hydrolaseDavid A Jacques
School of Molecular Bioscience, University of Sydney, Sydney, NSW 2006, Australia
Acta Crystallogr D Biol Crystallogr 67:105-11. 2011..However, no such activity could be identified and genetic analysis across species with known allophanate hydrolases indicates that a misannotation has occurred... - Calmodulin disrupts the structure of the HIV-1 MA proteinJohn Y H Chow
School of Molecular Bioscience, University of Sydney, New South Wales 2006, Australia
J Mol Biol 400:702-14. 2010..Thus, CaM binding is expected to impact upon the accessibility of interaction sites within MA that are involved in its various functions... - Solution structure studies of monomeric human TIP47/perilipin-3 reveal a highly extended conformationRobert M G Hynson
School of Molecular Bioscience, The University of Sydney, Sydney, New South Wales 2006, Australia
Proteins 80:2046-55. 2012..Moreover, we show that full-length TIP47/perilipin-3 adopts an extended conformation in solution, with considerable spatial separation of the N- and C-termini that would likely translate into a separation of functional domains... - Invited review: probing the structures of muscle regulatory proteins using small-angle solution scatteringYanling Lu
School of Molecular Bioscience, University of Sydney, NSW 2006, Australia
Biopolymers 95:505-16. 2011.... - Solution structure of the LIM-homeodomain transcription factor complex Lhx3/Ldb1 and the effects of a pituitary mutation on key Lhx3 interactionsMugdha Bhati
School of Molecular Bioscience, University of Sydney, Sydney, New South Wales, Australia
PLoS ONE 7:e40719. 2012..Thus the mutation would affect the formation of Lhx3-containing transcription factor complexes, particularly in the pituitary gland where these complexes are required for the production of multiple pituitary cell types and hormones... - Small-angle scattering for structural biology--expanding the frontier while avoiding the pitfallsDavid A Jacques
School of Molecular and Microbial Biosciences, The University of Sydney, Sydney, New South Wales 2006, Australia
Protein Sci 19:642-57. 2010..Examples of current best practice are given that also demonstrate the power of the technique to advance our understanding of protein structure and function... - The role of heme binding by DNA-protective protein from starved cells (Dps) in the Tolerance of Porphyromonas gingivalis to heme toxicityJin Long Gao
University of Sydney, New South Wales 2006, Australia
J Biol Chem 287:42243-58. 2012..gingivalis under excess heme as the only iron source. Conservation of the heme-coordinating cysteine among Dps isoforms from the Bacteroidales order suggests that this function may be a common feature within these anaerobic bacteria... - LIM domain binding proteins 1 and 2 have different oligomeric statesArwen J Cross
School of Molecular Bioscience, Building G08, The University of Sydney, NSW 2006, Australia
J Mol Biol 399:133-44. 2010..The abilities of Ldb1 and Ldb2 to form trimers and higher oligomers, respectively, should be considered in models of transcriptional regulation by Ldb1-containing complexes in a wide range of biological processes... - Publication guidelines for structural modelling of small-angle scattering data from biomolecules in solutionDavid A Jacques
School of Molecular Bioscience, The University of Sydney, NSW 2006, Australia
Acta Crystallogr D Biol Crystallogr 68:620-6. 2012..Here, these guidelines are outlined and the reasons for standardizing the way in which small-angle scattering data are presented... - Structure of the sporulation histidine kinase inhibitor Sda from Bacillus subtilis and insights into its solution stateDavid A Jacques
School of Molecular and Microbial Biosciences, University of Sydney, Australia
Acta Crystallogr D Biol Crystallogr 65:574-81. 2009..It also emphasizes the importance of understanding the physical principles behind these techniques and therefore their limitations... - A novel approach for enhancing the catalytic efficiency of a protease at low temperature: reduction in substrate inhibition by chemical modificationKhawar Sohail Siddiqui
School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia
Biotechnol Bioeng 103:676-86. 2009.... - Small-angle X-ray scattering reveals the N-terminal domain organization of cardiac myosin binding protein CCy M Jeffries
School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia
J Mol Biol 377:1186-99. 2008.... - Reliable structural interpretation of small-angle scattering data from bio-molecules in solution--the importance of quality control and a standard reporting frameworkDavid A Jacques
School of Molecular Bioscience Building, University of Sydney, G08 Butlin Avenue, Sydney, NSW 2006, Australia
BMC Struct Biol 12:9. 2012..Here we describe the rationale for these guidelines and the importance of standardising the way in which small-angle scattering data from bio-molecules and associated structural interpretations are reported... - A structural model of the catalytic subunit-regulatory subunit dimeric complex of the cAMP-dependent protein kinaseChang Shung Tung
Theoretical Biology and Biophysics Group and Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA
J Biol Chem 277:12423-31. 2002..This result is consistent with the known physiological events required for cAMP-dependent activation of the kinase... - Further insights into calmodulin-myosin light chain kinase interaction from solution scattering and shape restorationWilliam T Heller
Bioscience Division, MS M-888, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA
Biochemistry 42:10579-88. 2003..Our scattering data indicate that this step is completed only when all four calcium binding sites are loaded... - C subunits binding to the protein kinase A RI alpha dimer induce a large conformational changeWilliam T Heller
Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA
J Biol Chem 279:19084-90. 2004..In this model, the type I alpha holoenzyme forms a flattened V shape with the RI alpha dimerization domain at the point of the V and the cAMP-binding domains of the RI alpha subunits with their bound catalytic subunits at the ends... - Conformational differences among solution structures of the type Ialpha, IIalpha and IIbeta protein kinase A regulatory subunit homodimers: role of the linker regionsDominico Vigil
Department of Chemistry and Biochemistry and Howard Hughes Medical Institute, University of California, San Diego, La Jolla, CA 92037, USA
J Mol Biol 337:1183-94. 2004.... - Differential effects of substrate on type I and type II PKA holoenzyme dissociationDominico Vigil
Department of Chemistry and Biochemistry and Howard Hughes Medical Institute, University of California at San Diego, La Jolla, California 92037, USA
Biochemistry 43:5629-36. 2004..On the basis of these data and other recently published data, we propose a structural model of type I holoenzyme activation by cAMP... - Ca2+-induced structural changes in phosphorylase kinase detected by small-angle X-ray scatteringTimothy S Priddy
Department of Biochemistry and Molecular Biology, Mail Stop 3030, 3901 Rainbow Blvd, Kansas City, KS 66160, USA
Protein Sci 14:1039-48. 2005..Structural differences between the models representing the nonactivated and Ca(2+)-activated conformers of PhK are consistent with Ca(2+)-induced conformational changes in both the lobes and the interlobal bridges... - The conformationally dynamic C helix of the RIalpha subunit of protein kinase A mediates isoform-specific domain reorganization upon C subunit bindingDominico Vigil
Department of Chemistry and Biochemistry and Howard Hughes Medical Institute, University of California, San Diego, La Jolla, California 92037, USA
J Biol Chem 280:35521-7. 2005.... - Refinement of multidomain protein structures by combination of solution small-angle X-ray scattering and NMR dataAlexander Grishaev
Laboratory of Chemical Physics, NIDDK, National Institutes of Health, Bethesda, Maryland 20892 0520, USA
J Am Chem Soc 127:16621-8. 2005..07 A to 1.31 +/- 0.04 A. Combining SAXS data with NMR restraints can be accomplished at a moderate computational expense and is expected to become useful for multidomain proteins, multimeric assemblies, and tight macromolecular complexes... - Solution scattering reveals large differences in the global structures of type II protein kinase A isoformsDominico Vigil
Department of Chemistry and Biochemistry and Howard Hughes Medical Institute, University of California, San Diego, La Jolla, CA 92037, USA
J Mol Biol 357:880-9. 2006..The results provide an important structural foundation for understanding isoform-specific PKA localization and signaling... - The structure of the KinA-Sda complex suggests an allosteric mechanism of histidine kinase inhibitionAndrew E Whitten
Bragg Institute, Australian Nuclear Science and Technology Organisation, Lucas Heights 2234, Australia
J Mol Biol 368:407-20. 2007.... - Synaptic arrangement of the neuroligin/beta-neurexin complex revealed by X-ray and neutron scatteringDavide Comoletti
Department of Pharmacology, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA 92093, USA
Structure 15:693-705. 2007..As mutations of neurexin and neuroligin genes appear to be linked to autism, these models provide a structural framework for understanding altered recognition by these proteins in neurodevelopmental disorders... - Subdividing repressor function: DNA binding affinity, selectivity, and allostery can be altered by amino acid substitution of nonconserved residues in a LacI/GalR homologueHongli Zhan
Department of Biochemistry and Molecular Biology, MSN 3030, 3901 Rainbow Boulevard, The University of Kansas Medical Center, Kansas City, Kansas 66160, USA
Biochemistry 47:8058-69. 2008..Two more substitutions simultaneously diminish affinity, enhance allostery, and profoundly alter DNA ligand selectivity. Thus, positions within the linker can be varied to modulate different aspects of repressor function... - The solution structure of a cardiac troponin C-troponin I-troponin T complex shows a somewhat compact troponin C interacting with an extended troponin I-troponin T componentWilliam T Heller
Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA
Biochemistry 41:15654-63. 2002..A., and Trewhella, J. (1994) Biochemistry 33, 12800-12806]... - Solution structure of heavy meromyosin by small-angle scatteringSamantha P Harris
Department of Physiology, University of Wisconsin Medical School, Madison, Wisconsin 53706, USA
J Biol Chem 278:6034-40. 2003.... - Small-angle solution scattering reveals information on conformational dynamics in calcium-binding proteins and in their interactions with regulatory targetsJill Trewhella
Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM, USA
Methods Mol Biol 173:137-59. 2002 - Ligand-induced conformational changes and conformational dynamics in the solution structure of the lactose repressor proteinMarc Taraban
Department of Chemistry, University of Utah, Salt Lake City, UT 84112, USA
J Mol Biol 376:466-81. 2008..In gene regulation, such a flexible LacI would be beneficial for the interaction of its two DNA-binding domains, positioned at the tips of the V, with the required two of three LacI operators needed for full repression... - Mechanisms associated with cGMP binding and activation of cGMP-dependent protein kinaseMichael E Wall
Computer and Computational Sciences and Bioscience Divisions, Los Alamos National Laboratory, Los Alamos, NM 87545, USA
Proc Natl Acad Sci U S A 100:2380-5. 2003.... - Small-angle neutron scattering with contrast variation reveals spatial relationships between the three subunits in the ternary cardiac troponin complex and the effects of troponin I phosphorylationWilliam T Heller
Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA
Biochemistry 42:7790-800. 2003..The rodlike cTnI bends sharply at the end interacting with the cTnC/cTnT(198-298) component, which reorients so as to maintain its contacts with cTnI while undergoing only a relatively small change in shape... - Evolution of quaternary structure in a homotetrameric enzymeMichael D W Griffin
School of Biological Sciences, University of Canterbury, Christchurch 8140, New Zealand
J Mol Biol 380:691-703. 2008.... - Phosphorylation-dependent conformational transition of the cardiac specific N-extension of troponin I in cardiac troponinJack W Howarth
Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, Ohio, 45267, USA
J Mol Biol 373:706-22. 2007..This model provides a molecular mechanism for the observed changes in cross-bridge kinetics upon TnI phosphorylation... - Small-angle X-ray scattering of reduced ribonuclease A: effects of solution conditions and comparisons with a computational model of unfolded proteinsYuanyuan Wang
Department of Chemistry, University of Utah, 315 South 1400 East, Salt Lake City, UT 84112 0850, USA
J Mol Biol 377:1576-92. 2008..This type of collapse, therefore, is unlikely to greatly reduce the conformational search for the native state... - Ligand-induced conformational changes via flexible linkers in the amino-terminal region of the inositol 1,4,5-trisphosphate receptorJenny Chan
Division of Signaling Biology, Ontario Cancer Institute, Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada M5G 1L7
J Mol Biol 373:1269-80. 2007..IP(3) binding drives the conformational equilibrium toward more compact structures, while the presence of Ca2+ drives it to a more extended set... - Refined solution structure of the 82-kDa enzyme malate synthase G from joint NMR and synchrotron SAXS restraintsAlexander Grishaev
Laboratory of Chemical Physics, NIDDK, National Institutes of Health, Building 5, Bethesda, MD 20892 0520, USA
J Biomol NMR 40:95-106. 2008....
Research Grants
- STRUCTURAL CHANGES AND SIGNALLING IN CALCIUM REGULATORSJill Trewhella; Fiscal Year: 1992..The long term goal is to understand the molecular mechanism for calcium regulation...
- STRUCTURAL CHANGES IN SIGNALING IN CALCIUM REGULATIONSJill Trewhella; Fiscal Year: 2001..Understanding the underlying molecular mechanisms of regulation is thus of fundamental importance in health research. ..