Jill Trewhella

Summary

Affiliation: University of Sydney
Country: Australia

Publications

  1. pmc The different views from small angles
    Jill Trewhella
    School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia
    Proc Natl Acad Sci U S A 105:4967-8. 2008
  2. ncbi request reprint Structural themes and variations in protein kinase A as seen by small-angle scattering and neutron contrast variation
    Jill Trewhella
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia
    Eur Biophys J 35:585-9. 2006
  3. ncbi request reprint Protein kinase A targeting and activation as seen by small-angle solution scattering
    Jill Trewhella
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney 2006, NSW, Australia
    Eur J Cell Biol 85:655-62. 2006
  4. doi request reprint Histidine kinase regulation by a cyclophilin-like inhibitor
    David A Jacques
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, New South Wales 2006, Australia
    J Mol Biol 384:422-35. 2008
  5. doi request reprint A chemically modified alpha-amylase with a molten-globule state has entropically driven enhanced thermal stability
    Khawar Sohail Siddiqui
    School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia
    Protein Eng Des Sel 23:769-80. 2010
  6. doi request reprint The C0C1 fragment of human cardiac myosin binding protein C has common binding determinants for both actin and myosin
    Yanling Lu
    School of Molecular Bioscience, University of Sydney, NSW 2006, Australia
    J Mol Biol 413:908-13. 2011
  7. doi request reprint A novel structure of an antikinase and its inhibitor
    David A Jacques
    School of Molecular Bioscience, University of Sydney, NSW 2006, Australia
    J Mol Biol 405:214-26. 2011
  8. pmc Solution structure of the LIM-homeodomain transcription factor complex Lhx3/Ldb1 and the effects of a pituitary mutation on key Lhx3 interactions
    Mugdha Bhati
    School of Molecular Bioscience, University of Sydney, Sydney, New South Wales, Australia
    PLoS ONE 7:e40719. 2012
  9. doi request reprint Human cardiac myosin binding protein C: structural flexibility within an extended modular architecture
    Cy M Jeffries
    School of Molecular Bioscience, University of Sydney, New South Wales 2006, Australia
    J Mol Biol 414:735-48. 2011
  10. doi request reprint The structure of TTHA0988 from Thermus thermophilus, a KipI-KipA homologue incorrectly annotated as an allophanate hydrolase
    David A Jacques
    School of Molecular Bioscience, University of Sydney, Sydney, NSW 2006, Australia
    Acta Crystallogr D Biol Crystallogr 67:105-11. 2011

Research Grants

Collaborators

Detail Information

Publications46

  1. pmc The different views from small angles
    Jill Trewhella
    School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia
    Proc Natl Acad Sci U S A 105:4967-8. 2008
  2. ncbi request reprint Structural themes and variations in protein kinase A as seen by small-angle scattering and neutron contrast variation
    Jill Trewhella
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia
    Eur Biophys J 35:585-9. 2006
    ..These studies demonstrate the power of neutron contrast variation to expand our knowledge of the dynamic supra-molecular structures that carry out biological function...
  3. ncbi request reprint Protein kinase A targeting and activation as seen by small-angle solution scattering
    Jill Trewhella
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney 2006, NSW, Australia
    Eur J Cell Biol 85:655-62. 2006
    ....
  4. doi request reprint Histidine kinase regulation by a cyclophilin-like inhibitor
    David A Jacques
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, New South Wales 2006, Australia
    J Mol Biol 384:422-35. 2008
    ..Given that both KinA and KipI homologues are widespread in the bacterial kingdom, this mechanism has broad significance in bacterial signal transduction...
  5. doi request reprint A chemically modified alpha-amylase with a molten-globule state has entropically driven enhanced thermal stability
    Khawar Sohail Siddiqui
    School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia
    Protein Eng Des Sel 23:769-80. 2010
    ..This study provides new insight into the intriguing properties of an MG state resulting from the chemical modification of TAA...
  6. doi request reprint The C0C1 fragment of human cardiac myosin binding protein C has common binding determinants for both actin and myosin
    Yanling Lu
    School of Molecular Bioscience, University of Sydney, NSW 2006, Australia
    J Mol Biol 413:908-13. 2011
    ..Our results suggest that C0 and C1 interact with myosin and actin using a common set of binding determinants and therefore support a cMyBP-C switching mechanism between myosin and actin...
  7. doi request reprint A novel structure of an antikinase and its inhibitor
    David A Jacques
    School of Molecular Bioscience, University of Sydney, NSW 2006, Australia
    J Mol Biol 405:214-26. 2011
    ..We discuss the sequence conservation and possible roles across species of this near-ubiquitous protein family, which is poorly understood in terms of function...
  8. pmc Solution structure of the LIM-homeodomain transcription factor complex Lhx3/Ldb1 and the effects of a pituitary mutation on key Lhx3 interactions
    Mugdha Bhati
    School of Molecular Bioscience, University of Sydney, Sydney, New South Wales, Australia
    PLoS ONE 7:e40719. 2012
    ..Thus the mutation would affect the formation of Lhx3-containing transcription factor complexes, particularly in the pituitary gland where these complexes are required for the production of multiple pituitary cell types and hormones...
  9. doi request reprint Human cardiac myosin binding protein C: structural flexibility within an extended modular architecture
    Cy M Jeffries
    School of Molecular Bioscience, University of Sydney, New South Wales 2006, Australia
    J Mol Biol 414:735-48. 2011
    ..These structural features of cMyBP-C could facilitate its putative role as a molecular switch between actin and myosin and may contribute to modulating the transverse pliancy of the C-zone of the A-band across muscle sarcomeres...
  10. doi request reprint The structure of TTHA0988 from Thermus thermophilus, a KipI-KipA homologue incorrectly annotated as an allophanate hydrolase
    David A Jacques
    School of Molecular Bioscience, University of Sydney, Sydney, NSW 2006, Australia
    Acta Crystallogr D Biol Crystallogr 67:105-11. 2011
    ..However, no such activity could be identified and genetic analysis across species with known allophanate hydrolases indicates that a misannotation has occurred...
  11. pmc Calmodulin disrupts the structure of the HIV-1 MA protein
    John Y H Chow
    School of Molecular Bioscience, University of Sydney, New South Wales 2006, Australia
    J Mol Biol 400:702-14. 2010
    ..Thus, CaM binding is expected to impact upon the accessibility of interaction sites within MA that are involved in its various functions...
  12. pmc Solution structure studies of monomeric human TIP47/perilipin-3 reveal a highly extended conformation
    Robert M G Hynson
    School of Molecular Bioscience, The University of Sydney, Sydney, New South Wales 2006, Australia
    Proteins 80:2046-55. 2012
    ..Moreover, we show that full-length TIP47/perilipin-3 adopts an extended conformation in solution, with considerable spatial separation of the N- and C-termini that would likely translate into a separation of functional domains...
  13. doi request reprint Invited review: probing the structures of muscle regulatory proteins using small-angle solution scattering
    Yanling Lu
    School of Molecular Bioscience, University of Sydney, NSW 2006, Australia
    Biopolymers 95:505-16. 2011
    ....
  14. pmc Small-angle scattering for structural biology--expanding the frontier while avoiding the pitfalls
    David A Jacques
    School of Molecular and Microbial Biosciences, The University of Sydney, Sydney, New South Wales 2006, Australia
    Protein Sci 19:642-57. 2010
    ..Examples of current best practice are given that also demonstrate the power of the technique to advance our understanding of protein structure and function...
  15. doi request reprint Report of the wwPDB Small-Angle Scattering Task Force: data requirements for biomolecular modeling and the PDB
    Jill Trewhella
    School of Molecular Bioscience, The University of Sydney, NSW 2006, Australia
    Structure 21:875-81. 2013
    ....
  16. pmc Reliable structural interpretation of small-angle scattering data from bio-molecules in solution--the importance of quality control and a standard reporting framework
    David A Jacques
    School of Molecular Bioscience Building, University of Sydney, G08 Butlin Avenue, Sydney, NSW 2006, Australia
    BMC Struct Biol 12:9. 2012
    ..Here we describe the rationale for these guidelines and the importance of standardising the way in which small-angle scattering data from bio-molecules and associated structural interpretations are reported...
  17. pmc The role of heme binding by DNA-protective protein from starved cells (Dps) in the Tolerance of Porphyromonas gingivalis to heme toxicity
    Jin Long Gao
    University of Sydney, New South Wales 2006, Australia
    J Biol Chem 287:42243-58. 2012
    ..gingivalis under excess heme as the only iron source. Conservation of the heme-coordinating cysteine among Dps isoforms from the Bacteroidales order suggests that this function may be a common feature within these anaerobic bacteria...
  18. doi request reprint LIM domain binding proteins 1 and 2 have different oligomeric states
    Arwen J Cross
    School of Molecular Bioscience, Building G08, The University of Sydney, NSW 2006, Australia
    J Mol Biol 399:133-44. 2010
    ..The abilities of Ldb1 and Ldb2 to form trimers and higher oligomers, respectively, should be considered in models of transcriptional regulation by Ldb1-containing complexes in a wide range of biological processes...
  19. doi request reprint Publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution
    David A Jacques
    School of Molecular Bioscience, The University of Sydney, NSW 2006, Australia
    Acta Crystallogr D Biol Crystallogr 68:620-6. 2012
    ..Here, these guidelines are outlined and the reasons for standardizing the way in which small-angle scattering data are presented...
  20. doi request reprint A novel approach for enhancing the catalytic efficiency of a protease at low temperature: reduction in substrate inhibition by chemical modification
    Khawar Sohail Siddiqui
    School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia
    Biotechnol Bioeng 103:676-86. 2009
    ....
  21. pmc Structure of the sporulation histidine kinase inhibitor Sda from Bacillus subtilis and insights into its solution state
    David A Jacques
    School of Molecular and Microbial Biosciences, University of Sydney, Australia
    Acta Crystallogr D Biol Crystallogr 65:574-81. 2009
    ..It also emphasizes the importance of understanding the physical principles behind these techniques and therefore their limitations...
  22. doi request reprint Small-angle X-ray scattering reveals the N-terminal domain organization of cardiac myosin binding protein C
    Cy M Jeffries
    School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia
    J Mol Biol 377:1186-99. 2008
    ....
  23. doi request reprint K7del is a common TPM2 gene mutation associated with nemaline myopathy and raised myofibre calcium sensitivity
    Nancy Mokbel
    Institute for Neuroscience and Muscle Research, Children s Hospital at Westmead, Sydney, NSW 2145, Australia
    Brain 136:494-507. 2013
    ..These results suggest that patients may benefit from therapies that reduce skeletal muscle calcium sensitivity, and we highlight late muscle decompensation as an important cause of morbidity...
  24. pmc Subdividing repressor function: DNA binding affinity, selectivity, and allostery can be altered by amino acid substitution of nonconserved residues in a LacI/GalR homologue
    Hongli Zhan
    Department of Biochemistry and Molecular Biology, MSN 3030, 3901 Rainbow Boulevard, The University of Kansas Medical Center, Kansas City, Kansas 66160, USA
    Biochemistry 47:8058-69. 2008
    ..Two more substitutions simultaneously diminish affinity, enhance allostery, and profoundly alter DNA ligand selectivity. Thus, positions within the linker can be varied to modulate different aspects of repressor function...
  25. pmc Ca2+-induced structural changes in phosphorylase kinase detected by small-angle X-ray scattering
    Timothy S Priddy
    Department of Biochemistry and Molecular Biology, Mail Stop 3030, 3901 Rainbow Blvd, Kansas City, KS 66160, USA
    Protein Sci 14:1039-48. 2005
    ..Structural differences between the models representing the nonactivated and Ca(2+)-activated conformers of PhK are consistent with Ca(2+)-induced conformational changes in both the lobes and the interlobal bridges...
  26. ncbi request reprint Differential effects of substrate on type I and type II PKA holoenzyme dissociation
    Dominico Vigil
    Department of Chemistry and Biochemistry and Howard Hughes Medical Institute, University of California at San Diego, La Jolla, California 92037, USA
    Biochemistry 43:5629-36. 2004
    ..On the basis of these data and other recently published data, we propose a structural model of type I holoenzyme activation by cAMP...
  27. ncbi request reprint Conformational differences among solution structures of the type Ialpha, IIalpha and IIbeta protein kinase A regulatory subunit homodimers: role of the linker regions
    Dominico Vigil
    Department of Chemistry and Biochemistry and Howard Hughes Medical Institute, University of California, San Diego, La Jolla, CA 92037, USA
    J Mol Biol 337:1183-94. 2004
    ....
  28. ncbi request reprint C subunits binding to the protein kinase A RI alpha dimer induce a large conformational change
    William T Heller
    Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA
    J Biol Chem 279:19084-90. 2004
    ..In this model, the type I alpha holoenzyme forms a flattened V shape with the RI alpha dimerization domain at the point of the V and the cAMP-binding domains of the RI alpha subunits with their bound catalytic subunits at the ends...
  29. ncbi request reprint Further insights into calmodulin-myosin light chain kinase interaction from solution scattering and shape restoration
    William T Heller
    Bioscience Division, MS M 888, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA
    Biochemistry 42:10579-88. 2003
    ..Our scattering data indicate that this step is completed only when all four calcium binding sites are loaded...
  30. pmc Mechanisms associated with cGMP binding and activation of cGMP-dependent protein kinase
    Michael E Wall
    Computer and Computational Sciences and Bioscience Divisions, Los Alamos National Laboratory, Los Alamos, NM 87545, USA
    Proc Natl Acad Sci U S A 100:2380-5. 2003
    ....
  31. ncbi request reprint The solution structure of a cardiac troponin C-troponin I-troponin T complex shows a somewhat compact troponin C interacting with an extended troponin I-troponin T component
    William T Heller
    Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA
    Biochemistry 41:15654-63. 2002
    ..A., and Trewhella, J. (1994) Biochemistry 33, 12800-12806]...
  32. ncbi request reprint Solution structure of heavy meromyosin by small-angle scattering
    Samantha P Harris
    Department of Physiology, University of Wisconsin Medical School, Madison, Wisconsin 53706, USA
    J Biol Chem 278:6034-40. 2003
    ....
  33. ncbi request reprint Small-angle solution scattering reveals information on conformational dynamics in calcium-binding proteins and in their interactions with regulatory targets
    Jill Trewhella
    Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM, USA
    Methods Mol Biol 173:137-59. 2002
  34. ncbi request reprint A structural model of the catalytic subunit-regulatory subunit dimeric complex of the cAMP-dependent protein kinase
    Chang Shung Tung
    Theoretical Biology and Biophysics Group and Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA
    J Biol Chem 277:12423-31. 2002
    ..This result is consistent with the known physiological events required for cAMP-dependent activation of the kinase...
  35. ncbi request reprint The conformationally dynamic C helix of the RIalpha subunit of protein kinase A mediates isoform-specific domain reorganization upon C subunit binding
    Dominico Vigil
    Department of Chemistry and Biochemistry and Howard Hughes Medical Institute, University of California, San Diego, La Jolla, California 92037, USA
    J Biol Chem 280:35521-7. 2005
    ....
  36. ncbi request reprint Refinement of multidomain protein structures by combination of solution small-angle X-ray scattering and NMR data
    Alexander Grishaev
    Laboratory of Chemical Physics, NIDDK, National Institutes of Health, Bethesda, Maryland 20892 0520, USA
    J Am Chem Soc 127:16621-8. 2005
    ..07 A to 1.31 +/- 0.04 A. Combining SAXS data with NMR restraints can be accomplished at a moderate computational expense and is expected to become useful for multidomain proteins, multimeric assemblies, and tight macromolecular complexes...
  37. ncbi request reprint Solution scattering reveals large differences in the global structures of type II protein kinase A isoforms
    Dominico Vigil
    Department of Chemistry and Biochemistry and Howard Hughes Medical Institute, University of California, San Diego, La Jolla, CA 92037, USA
    J Mol Biol 357:880-9. 2006
    ..The results provide an important structural foundation for understanding isoform-specific PKA localization and signaling...
  38. ncbi request reprint The structure of the KinA-Sda complex suggests an allosteric mechanism of histidine kinase inhibition
    Andrew E Whitten
    Bragg Institute, Australian Nuclear Science and Technology Organisation, Lucas Heights 2234, Australia
    J Mol Biol 368:407-20. 2007
    ....
  39. pmc Synaptic arrangement of the neuroligin/beta-neurexin complex revealed by X-ray and neutron scattering
    Davide Comoletti
    Department of Pharmacology, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA 92093, USA
    Structure 15:693-705. 2007
    ..As mutations of neurexin and neuroligin genes appear to be linked to autism, these models provide a structural framework for understanding altered recognition by these proteins in neurodevelopmental disorders...
  40. pmc Ligand-induced conformational changes and conformational dynamics in the solution structure of the lactose repressor protein
    Marc Taraban
    Department of Chemistry, University of Utah, Salt Lake City, UT 84112, USA
    J Mol Biol 376:466-81. 2008
    ..In gene regulation, such a flexible LacI would be beneficial for the interaction of its two DNA-binding domains, positioned at the tips of the V, with the required two of three LacI operators needed for full repression...
  41. doi request reprint Evolution of quaternary structure in a homotetrameric enzyme
    Michael D W Griffin
    School of Biological Sciences, University of Canterbury, Christchurch 8140, New Zealand
    J Mol Biol 380:691-703. 2008
    ....
  42. ncbi request reprint Refined solution structure of the 82-kDa enzyme malate synthase G from joint NMR and synchrotron SAXS restraints
    Alexander Grishaev
    Laboratory of Chemical Physics, NIDDK, National Institutes of Health, Building 5, Bethesda, MD 20892 0520, USA
    J Biomol NMR 40:95-106. 2008
    ....
  43. ncbi request reprint Phosphorylation-dependent conformational transition of the cardiac specific N-extension of troponin I in cardiac troponin
    Jack W Howarth
    Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, Ohio, 45267, USA
    J Mol Biol 373:706-22. 2007
    ..This model provides a molecular mechanism for the observed changes in cross-bridge kinetics upon TnI phosphorylation...
  44. pmc Small-angle X-ray scattering of reduced ribonuclease A: effects of solution conditions and comparisons with a computational model of unfolded proteins
    Yuanyuan Wang
    Department of Chemistry, University of Utah, 315 South 1400 East, Salt Lake City, UT 84112 0850, USA
    J Mol Biol 377:1576-92. 2008
    ..This type of collapse, therefore, is unlikely to greatly reduce the conformational search for the native state...
  45. ncbi request reprint Ligand-induced conformational changes via flexible linkers in the amino-terminal region of the inositol 1,4,5-trisphosphate receptor
    Jenny Chan
    Division of Signaling Biology, Ontario Cancer Institute, Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada M5G 1L7
    J Mol Biol 373:1269-80. 2007
    ..IP(3) binding drives the conformational equilibrium toward more compact structures, while the presence of Ca2+ drives it to a more extended set...
  46. ncbi request reprint Small-angle neutron scattering with contrast variation reveals spatial relationships between the three subunits in the ternary cardiac troponin complex and the effects of troponin I phosphorylation
    William T Heller
    Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA
    Biochemistry 42:7790-800. 2003
    ..The rodlike cTnI bends sharply at the end interacting with the cTnC/cTnT(198-298) component, which reorients so as to maintain its contacts with cTnI while undergoing only a relatively small change in shape...

Research Grants13

  1. STRUCTURAL CHANGES AND SIGNALLING IN CALCIUM REGULATORS
    Jill Trewhella; Fiscal Year: 1992
    ..The long term goal is to understand the molecular mechanism for calcium regulation...
  2. STRUCTURAL CHANGES IN SIGNALING IN CALCIUM REGULATIONS
    Jill Trewhella; Fiscal Year: 2001
    ..Understanding the underlying molecular mechanisms of regulation is thus of fundamental importance in health research. ..