Doug W Smith
Affiliation: University of Newcastle
- Purity and enrichment of laser-microdissected midbrain dopamine neuronsAmanda L Brown
Neurobiology of Ageing Laboratory, School of Biomedical Sciences and Pharmacy, Centre for Translational Neuroscience and Mental Health Research, University of Newcastle, Newcastle, NSW 2308, Australia
Biomed Res Int 2013:747938. 2013..This study demonstrates that it is possible to laser-microdissect dopamine neurons to a high degree of cell purity. Therefore gene expression profiles can be precisely attributed to the targeted microdissected cells. ..
- Characterisation of mitochondrial DNA deletions by long-PCR in central nervous system regions of young, middle- and old-aged ratsAshley Cahif
School of Biomedical Sciences and Pharmacy, Medical Sciences Building, Room 306C, University of Newcastle, Callaghan, NSW 2308, Australia
Curr Aging Sci 6:232-8. 2013..These findings are remarkably consistent with previous human brain data and further underscore the value of the rat model for investigation of ageing-related changes in the mitochondrial genome. ..
- Role of neuronal nitric oxide in the dopamine deficit of HPRT-deficient miceDoug W Smith
Center for Molecular Genetics, Department of Pediatrics, University of California, San Diego, CA, USA
Metab Brain Dis 22:39-43. 2007..These results indicate that NOS1 produced nitric oxide does not contribute to the dopamine deficit seen in HPRT deficiency...
- Down-regulated striatal gene expression for synaptic plasticity-associated proteins in addiction and relapse vulnerable animalsAmanda L Brown
Neurobiology of Addiction Laboratory, School of Biomedical Sciences and Pharmacy, Centre for Brain and Mental Health Research, University of Newcastle and the Hunter Medical Research Institute, Newcastle, NSW, Australia
Int J Neuropsychopharmacol 14:1099-110. 2011....
- mTORC1 inhibition in the nucleus accumbens 'protects' against the expression of drug seeking and 'relapse' and is associated with reductions in GluA1 AMPAR and CAMKIIα levelsMorgan H James
Neurobiology of Addiction Laboratory, School of Biomedical Sciences and Pharmacy and the Centre for Translational Neuroscience and Mental Health Research, University of Newcastle and the Hunter Medical Research Institute, Newcastle, NSW, Australia
Neuropsychopharmacology 39:1694-702. 2014..These effects appear to involve a role for mTORC1 in the regulation of GluA1 AMPARs and CAMKIIα in the NACsh. ..
- Altered expression of histone and synaptic plasticity associated genes in the hippocampus of streptozotocin-induced diabetic miceJency Thomas
University of Newcastle, Callaghan, NSW, Australia
Metab Brain Dis 28:613-8. 2013..The present study will improve our understanding of the neural mechanisms that might underpin diabetes-related cognitive dysfunction. ..
- Improved RNA preservation for immunolabeling and laser microdissectionAmanda L Brown
Faculty of Health, School of Biomedical Sciences and Pharmacy, University of Newcastle, Newcastle NSW 2308, Australia
RNA 15:2364-74. 2009..Our described method yields high quality RNA from immunolabeled microdissected cells, an essential requirement for meaningful genomics investigations of normal and pathological cells isolated from complex tissues...
- Fluoxetine prevents development of an early stress-related molecular signature in the rat infralimbic medial prefrontal cortex. Implications for depression?Rafael A Barreto
School of Biomedical Sciences and Pharmacy Faculty of Health, University of Newcastle, Callaghan, NSW, 2308, Australia
BMC Neurosci 13:125. 2012..The ILmPFC is thought to be involved in the processing of emotionally contextual information and in orchestrating the related autonomic responses, and it is one of the brain regions implicated in both stress responses and depression...
- Orexin-1 receptor signalling within the ventral tegmental area, but not the paraventricular thalamus, is critical to regulating cue-induced reinstatement of cocaine-seekingMorgan H James
Neurobiology of Addiction Laboratory, School of Biomedical Sciences and Pharmacy and the Centre for Brain and Mental Health Research, University of Newcastle and the Hunter Medical Research Institute, Newcastle, NSW, Australia
Int J Neuropsychopharmacol 14:684-90. 2011..We hypothesize that blockade of VTA OrxR1 signalling may reduce nucleus accumbens dopamine in response to drug cue presentation...
- Discrepant effects of culture conditions on survival and function of dopaminergic neuronsDoug W Smith
Center for Molecular Genetics, Department of Pediatrics, School of Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
Neuroreport 15:1025-8. 2004..These results demonstrate that dopaminergic neurons can be grown in serum-free conditions but that the choice of culture conditions has a marked influence on cell survival and function...
- Region-specific changes in mitochondrial D-loop in aged rat CNSSimone C McInerny
Discipline of Anatomy, School of Biomedical Sciences, Faculty of Health and The Center for Brain and Mental Health Research, University of Newcastle, Callaghan, NSW 2308, Australia
Mech Ageing Dev 130:343-9. 2009..Mitochondrial genome copy number varied between regions, in addition to an overall 18% decrease with age across the whole brain. These results suggest the age-related decline in OXPHOS may be related to a reduction in D-loop function...
- An emerging role for the Mammalian target of rapamycin in "pathological" protein translation: relevance to cocaine addictionChristopher V Dayas
School of Biomedical Sciences and Pharmacy, Centre for Translational Neuroscience and Mental Health Research, Hunter Medical Research Institute, University of Newcastle Callaghan, NSW, Australia
Front Pharmacol 3:13. 2012....
- Oxidative stress and dopamine deficiency in a genetic mouse model of Lesch-Nyhan diseaseJasper E Visser
Department of Neurology, Meyer 6 181, Johns Hopkins Hospital, Baltimore, MD 21287, USA
Brain Res Dev Brain Res 133:127-39. 2002..Overall, these data provide evidence for increased oxidative stress, but the failure to protect the knockout mice by over-expressing SOD1 argues that oxidative stress is not the sole process responsible for the loss of striatal dopamine...