Khawar Sohail Siddiqui

Summary

Affiliation: University of New South Wales
Location: Sydney, Australia
Summary:
Protein structure-function-stability relationship, Enzymology, Chemical modification of proteins, bioremediation, extremophilic enzymes, Industrial and environmental biotechnology, analytical protein chemistryand protein stability

Publications

  1. ncbi request reprint A novel thermodynamic relationship based on Kramers Theory for studying enzyme kinetics under high viscosity
    Khawar Sohail Siddiqui
    School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia
    IUBMB Life 56:403-7. 2004
  2. ncbi request reprint Improved activity and stability of alkaline phosphatases from psychrophilic and mesophilic organisms by chemically modifying aliphatic or amino groups using tetracarboxy-benzophenone derivatives
    K S Siddiqui
    School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia
    Cell Mol Biol (Noisy-le-grand) 50:657-67. 2004
  3. doi request reprint Chaperonins from an Antarctic archaeon are predominantly monomeric: crystal structure of an open state monomer
    Oliver Pilak
    School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, New South Wales 2052, Australia
    Environ Microbiol 13:2232-49. 2011
  4. doi request reprint The RNA polymerase subunits E/F from the Antarctic archaeon Methanococcoides burtonii bind to specific species of mRNA
    Davide De Francisci
    School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia
    Environ Microbiol 13:2039-55. 2011
  5. doi request reprint A novel prokaryotic L-arginine:glycine amidinotransferase is involved in cylindrospermopsin biosynthesis
    Julia Muenchhoff
    School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, Australia
    FEBS J 277:3844-60. 2010
  6. doi request reprint The genome sequence of the psychrophilic archaeon, Methanococcoides burtonii: the role of genome evolution in cold adaptation
    Michelle A Allen
    School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, New South Wales, Australia
    ISME J 3:1012-35. 2009
  7. pmc Structure and function of cold shock proteins in archaea
    Laura Giaquinto
    School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, New South Wales 2052, Australia
    J Bacteriol 189:5738-48. 2007
  8. ncbi request reprint Improved thermal stability and activity in the cold-adapted lipase B from Candida antarctica following chemical modification with oxidized polysaccharides
    Khawar Sohail Siddiqui
    School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, 2052, NSW, Australia
    Extremophiles 9:471-6. 2005
  9. pmc Role of disulfide bridges in the activity and stability of a cold-active alpha-amylase
    Khawar Sohail Siddiqui
    School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales 2052, Australia
    J Bacteriol 187:6206-12. 2005
  10. doi request reprint A novel approach for enhancing the catalytic efficiency of a protease at low temperature: reduction in substrate inhibition by chemical modification
    Khawar Sohail Siddiqui
    School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia
    Biotechnol Bioeng 103:676-86. 2009

Collaborators

Detail Information

Publications16

  1. ncbi request reprint A novel thermodynamic relationship based on Kramers Theory for studying enzyme kinetics under high viscosity
    Khawar Sohail Siddiqui
    School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia
    IUBMB Life 56:403-7. 2004
    ..These could be very useful for determining the thermodynamics of enzymes catalyzing reactions at temperature extremes in the presence of substrate solutions of different compositions and viscosities...
  2. ncbi request reprint Improved activity and stability of alkaline phosphatases from psychrophilic and mesophilic organisms by chemically modifying aliphatic or amino groups using tetracarboxy-benzophenone derivatives
    K S Siddiqui
    School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia
    Cell Mol Biol (Noisy-le-grand) 50:657-67. 2004
    ..The study demonstrates the applicability of benzophenone chemical modification for improving the thermal properties of enzymes from psychrophiles and mesophiles...
  3. doi request reprint Chaperonins from an Antarctic archaeon are predominantly monomeric: crystal structure of an open state monomer
    Oliver Pilak
    School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, New South Wales 2052, Australia
    Environ Microbiol 13:2232-49. 2011
    ..The predominance of monomeric form and the ability to adopt a fully open nucleotide site appear to be unique features of the M. burtonii group II chaperonins...
  4. doi request reprint The RNA polymerase subunits E/F from the Antarctic archaeon Methanococcoides burtonii bind to specific species of mRNA
    Davide De Francisci
    School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia
    Environ Microbiol 13:2039-55. 2011
    ..By using MbRpoE/F to capture and characterize cellular RNA we have identified a previously unknown functional property of the MbRpoE/F heterodimer...
  5. doi request reprint A novel prokaryotic L-arginine:glycine amidinotransferase is involved in cylindrospermopsin biosynthesis
    Julia Muenchhoff
    School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, Australia
    FEBS J 277:3844-60. 2010
    ....
  6. doi request reprint The genome sequence of the psychrophilic archaeon, Methanococcoides burtonii: the role of genome evolution in cold adaptation
    Michelle A Allen
    School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, New South Wales, Australia
    ISME J 3:1012-35. 2009
    ....
  7. pmc Structure and function of cold shock proteins in archaea
    Laura Giaquinto
    School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, New South Wales 2052, Australia
    J Bacteriol 189:5738-48. 2007
    ..The initial biochemical analysis of M. frigidum Csp has developed a platform for further characterization and demonstrates the potential for expanding molecular studies of proteins from this important archaeal stenopsychrophile...
  8. ncbi request reprint Improved thermal stability and activity in the cold-adapted lipase B from Candida antarctica following chemical modification with oxidized polysaccharides
    Khawar Sohail Siddiqui
    School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, 2052, NSW, Australia
    Extremophiles 9:471-6. 2005
    ....
  9. pmc Role of disulfide bridges in the activity and stability of a cold-active alpha-amylase
    Khawar Sohail Siddiqui
    School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales 2052, Australia
    J Bacteriol 187:6206-12. 2005
    ..Overall, the study demonstrated that none of the four disulfide bonds are important in stabilizing the native structure of enzyme, and instead, they appear to promote a localized destabilization to preserve activity...
  10. doi request reprint A novel approach for enhancing the catalytic efficiency of a protease at low temperature: reduction in substrate inhibition by chemical modification
    Khawar Sohail Siddiqui
    School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia
    Biotechnol Bioeng 103:676-86. 2009
    ....
  11. ncbi request reprint Role of lysine versus arginine in enzyme cold-adaptation: modifying lysine to homo-arginine stabilizes the cold-adapted alpha-amylase from Pseudoalteramonas haloplanktis
    Khawar Sohail Siddiqui
    School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW, Australia
    Proteins 64:486-501. 2006
    ..These data were consistent with computational analyses that show that AHA possesses a compositional bias that favors decreased conformational stability and increased flexibility...
  12. ncbi request reprint Thermodynamic activation properties of elongation factor 2 (EF-2) proteins from psychrotolerant and thermophilic Archaea
    Khawar Sohail Siddiqui
    Microbiology and Immunology, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia
    Extremophiles 6:143-50. 2002
    ....
  13. ncbi request reprint Cold-adapted enzymes
    Khawar Sohail Siddiqui
    School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia
    Annu Rev Biochem 75:403-33. 2006
    ..Because of the unique properties of cold-adapted enzymes, they are not only an important focus in extremophile biology, but also represent a valuable model for fundamental research into protein folding and catalysis...
  14. doi request reprint A chemically modified alpha-amylase with a molten-globule state has entropically driven enhanced thermal stability
    Khawar Sohail Siddiqui
    School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia
    Protein Eng Des Sel 23:769-80. 2010
    ..This study provides new insight into the intriguing properties of an MG state resulting from the chemical modification of TAA...
  15. ncbi request reprint Kinetic and thermodynamic study of a chemically modified highly active xylanase from Scopulariopsis sp: existence of an essential amino group
    Ahmed Jawaard Afzal
    Department of Molecular Biology, Microbiology and Biochemistry, Southern Illinois University at Carbondale, Carbondale, IL 62901, USA corrected
    Appl Biochem Biotechnol 141:273-97. 2007
    ..These results suggest that the altered properties of the modified enzymes were a result of the conformational changes brought about by chemical modification...
  16. ncbi request reprint Innovative kinetic and thermodynamic analysis of a purified superactive xylanase from Scopulariopsis sp
    Ahmed Jawaad Afzal
    Department of Molecular Biology, Southern Illinois University at Carbondale, Carbondale, IL 62901, USA
    Appl Biochem Biotechnol 120:51-70. 2005
    ..The simultaneously thermostable and highly active LAX could be utilized in biotechnological processes involving xylan hydrolysis...