Research Topics
| Khawar Sohail SiddiquiSummaryAffiliation: University of New South Wales Location: Sydney, Australia Summary: Protein structure-function-stability relationship, Enzymology, Chemical modification of proteins, bioremediation, extremophilic enzymes, Industrial and environmental biotechnology, analytical protein chemistryand protein stability Publications
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Detail Information
Publications
A novel thermodynamic relationship based on Kramers Theory for studying enzyme kinetics under high viscosityKhawar Sohail Siddiqui
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia
IUBMB Life 56:403-7. 2004..These could be very useful for determining the thermodynamics of enzymes catalyzing reactions at temperature extremes in the presence of substrate solutions of different compositions and viscosities...
Chaperonins from an Antarctic archaeon are predominantly monomeric: crystal structure of an open state monomerOliver Pilak
School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, New South Wales 2052, Australia
Environ Microbiol 13:2232-49. 2011..The predominance of monomeric form and the ability to adopt a fully open nucleotide site appear to be unique features of the M. burtonii group II chaperonins...- The RNA polymerase subunits E/F from the Antarctic archaeon Methanococcoides burtonii bind to specific species of mRNADavide De Francisci
School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia
Environ Microbiol 13:2039-55. 2011..By using MbRpoE/F to capture and characterize cellular RNA we have identified a previously unknown functional property of the MbRpoE/F heterodimer... - A novel prokaryotic L-arginine:glycine amidinotransferase is involved in cylindrospermopsin biosynthesisJulia Muenchhoff
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, Australia
FEBS J 277:3844-60. 2010.... - The genome sequence of the psychrophilic archaeon, Methanococcoides burtonii: the role of genome evolution in cold adaptationMichelle A Allen
School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, New South Wales, Australia
ISME J 3:1012-35. 2009.... 
Structure and function of cold shock proteins in archaeaLaura Giaquinto
School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, New South Wales 2052, Australia
J Bacteriol 189:5738-48. 2007..The initial biochemical analysis of M. frigidum Csp has developed a platform for further characterization and demonstrates the potential for expanding molecular studies of proteins from this important archaeal stenopsychrophile...- Improved activity and stability of alkaline phosphatases from psychrophilic and mesophilic organisms by chemically modifying aliphatic or amino groups using tetracarboxy-benzophenone derivativesK S Siddiqui
School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia
Cell Mol Biol (Noisy-le-grand) 50:657-67. 2004..The study demonstrates the applicability of benzophenone chemical modification for improving the thermal properties of enzymes from psychrophiles and mesophiles... - Improved thermal stability and activity in the cold-adapted lipase B from Candida antarctica following chemical modification with oxidized polysaccharidesKhawar Sohail Siddiqui
School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, 2052, NSW, Australia
Extremophiles 9:471-6. 2005.... - Role of disulfide bridges in the activity and stability of a cold-active alpha-amylaseKhawar Sohail Siddiqui
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales 2052, Australia
J Bacteriol 187:6206-12. 2005..Overall, the study demonstrated that none of the four disulfide bonds are important in stabilizing the native structure of enzyme, and instead, they appear to promote a localized destabilization to preserve activity... - A novel approach for enhancing the catalytic efficiency of a protease at low temperature: reduction in substrate inhibition by chemical modificationKhawar Sohail Siddiqui
School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia
Biotechnol Bioeng 103:676-86. 2009.... - Role of lysine versus arginine in enzyme cold-adaptation: modifying lysine to homo-arginine stabilizes the cold-adapted alpha-amylase from Pseudoalteramonas haloplanktisKhawar Sohail Siddiqui
School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW, Australia
Proteins 64:486-501. 2006..These data were consistent with computational analyses that show that AHA possesses a compositional bias that favors decreased conformational stability and increased flexibility... - Thermodynamic activation properties of elongation factor 2 (EF-2) proteins from psychrotolerant and thermophilic ArchaeaKhawar Sohail Siddiqui
Microbiology and Immunology, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia
Extremophiles 6:143-50. 2002.... - Cold-adapted enzymesKhawar Sohail Siddiqui
School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia
Annu Rev Biochem 75:403-33. 2006..Because of the unique properties of cold-adapted enzymes, they are not only an important focus in extremophile biology, but also represent a valuable model for fundamental research into protein folding and catalysis... - A chemically modified alpha-amylase with a molten-globule state has entropically driven enhanced thermal stabilityKhawar Sohail Siddiqui
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia
Protein Eng Des Sel 23:769-80. 2010..This study provides new insight into the intriguing properties of an MG state resulting from the chemical modification of TAA... - Kinetic and thermodynamic study of a chemically modified highly active xylanase from Scopulariopsis sp: existence of an essential amino groupAhmed Jawaard Afzal
Department of Molecular Biology, Microbiology and Biochemistry, Southern Illinois University at Carbondale, Carbondale, IL 62901, USA corrected
Appl Biochem Biotechnol 141:273-97. 2007..These results suggest that the altered properties of the modified enzymes were a result of the conformational changes brought about by chemical modification... - Innovative kinetic and thermodynamic analysis of a purified superactive xylanase from Scopulariopsis spAhmed Jawaad Afzal
Department of Molecular Biology, Southern Illinois University at Carbondale, Carbondale, IL 62901, USA
Appl Biochem Biotechnol 120:51-70. 2005..The simultaneously thermostable and highly active LAX could be utilized in biotechnological processes involving xylan hydrolysis...