Dylan M Owen

Summary

Affiliation: University of New South Wales
Country: Australia

Publications

  1. ncbi Optimized time-gated generalized polarization imaging of Laurdan and di-4-ANEPPDHQ for membrane order image contrast enhancement
    Dylan M Owen
    Centre for Vascular Research, University of New South Wales, Sydney, Australia
    Microsc Res Tech 73:618-22. 2010
  2. doi Quantitative imaging of membrane lipid order in cells and organisms
    Dylan M Owen
    Centre for Vascular Research, University of New South Wales, Sydney, Australia
    Nat Protoc 7:24-35. 2012
  3. doi Super-resolution imaging by localization microscopy
    Dylan M Owen
    Lowy Cancer Research Centre, Centre for Vascular Research, University of New South Wales, Sydney, Australia
    Methods Mol Biol 950:81-93. 2013
  4. doi The lipid raft hypothesis revisited--new insights on raft composition and function from super-resolution fluorescence microscopy
    Dylan M Owen
    Centre for Vascular Research and Australian Centre for NanoMedicine, University of New South Wales, Sydney, Australia
    Bioessays 34:739-47. 2012
  5. doi PALM imaging and cluster analysis of protein heterogeneity at the cell surface
    Dylan M Owen
    Centre for Vascular Research, University of New South Wales, Sydney, New South Wales, Australia
    J Biophotonics 3:446-54. 2010
  6. doi Sub-resolution lipid domains exist in the plasma membrane and regulate protein diffusion and distribution
    Dylan M Owen
    Centre for Vascular Research and Australian Centre for NanoMedicine, University of New South Wales, High Street, Sydney, New South Wales 2052, Australia
    Nat Commun 3:1256. 2012
  7. doi Optical techniques for imaging membrane domains in live cells (live-cell palm of protein clustering)
    Dylan M Owen
    Centre for Vascular Research, University of New South Wales, Sydney, Australia
    Methods Enzymol 504:221-35. 2012
  8. pmc Characterization of a new series of fluorescent probes for imaging membrane order
    Joanna M Kwiatek
    Centre for Vascular Research and Australian Centre for NanoMedicine, University of New South Wales, Sydney, Australia
    PLoS ONE 8:e52960. 2013
  9. pmc Imaging membrane lipid order in whole, living vertebrate organisms
    Dylan M Owen
    Center for Vascular Research, University of New South Wales, Sydney, Australia
    Biophys J 99:L7-9. 2010
  10. ncbi Quantitative microscopy: protein dynamics and membrane organisation
    Dylan M Owen
    Centre for Vascular Research, University of New South Wales, and the Department of Haematology, Prince of Wales Hospital, Sydney, Australia
    Traffic 10:962-71. 2009

Collaborators

Detail Information

Publications17

  1. ncbi Optimized time-gated generalized polarization imaging of Laurdan and di-4-ANEPPDHQ for membrane order image contrast enhancement
    Dylan M Owen
    Centre for Vascular Research, University of New South Wales, Sydney, Australia
    Microsc Res Tech 73:618-22. 2010
    ..This improvement in image contrast enhancement will be invaluable when studying the role of lipid rafts in fixed and live cell systems...
  2. doi Quantitative imaging of membrane lipid order in cells and organisms
    Dylan M Owen
    Centre for Vascular Research, University of New South Wales, Sydney, Australia
    Nat Protoc 7:24-35. 2012
    ..This approach, which takes around 4 h, directly examines the organization of cellular membranes and is distinct from alternative approaches that infer membrane order by measuring probe partitioning or dynamics...
  3. doi Super-resolution imaging by localization microscopy
    Dylan M Owen
    Lowy Cancer Research Centre, Centre for Vascular Research, University of New South Wales, Sydney, Australia
    Methods Mol Biol 950:81-93. 2013
    ..In this example, the system under investigation will be fixed HeLa cells transfected with the photo-switchable fluorescent protein PS-CFP2 targeted to the plasma membrane by fusion to the N-terminus of the protein kinase Lck...
  4. doi The lipid raft hypothesis revisited--new insights on raft composition and function from super-resolution fluorescence microscopy
    Dylan M Owen
    Centre for Vascular Research and Australian Centre for NanoMedicine, University of New South Wales, Sydney, Australia
    Bioessays 34:739-47. 2012
    ..This review describes such techniques, particularly as applied to the study of membrane organisation, synthesising newly emerging facets of lipid raft biology into a state-of-the art model...
  5. doi PALM imaging and cluster analysis of protein heterogeneity at the cell surface
    Dylan M Owen
    Centre for Vascular Research, University of New South Wales, Sydney, New South Wales, Australia
    J Biophotonics 3:446-54. 2010
    ..These methods are generally applicable to other cell and fluorophore systems to quantify 2-D molecular clustering at nanometer scales...
  6. doi Sub-resolution lipid domains exist in the plasma membrane and regulate protein diffusion and distribution
    Dylan M Owen
    Centre for Vascular Research and Australian Centre for NanoMedicine, University of New South Wales, High Street, Sydney, New South Wales 2052, Australia
    Nat Commun 3:1256. 2012
    ..Our data therefore propose a membrane model in which the majority of the plasma membrane is covered by cholesterol-dependent, ordered lipid domains that contribute to the non-random distribution and diffusion of membrane constituents...
  7. doi Optical techniques for imaging membrane domains in live cells (live-cell palm of protein clustering)
    Dylan M Owen
    Centre for Vascular Research, University of New South Wales, Sydney, Australia
    Methods Enzymol 504:221-35. 2012
    ..J., Owen, D. M., Rossy, J., Magenau, A., Wehrmann, M., Gooding, J. J. , and Gaus, K. (2011). Pre-existing clusters of the adaptor Lat do not participate in early T cell signaling events. Nat. Immunol.12, 655-662.]...
  8. pmc Characterization of a new series of fluorescent probes for imaging membrane order
    Joanna M Kwiatek
    Centre for Vascular Research and Australian Centre for NanoMedicine, University of New South Wales, Sydney, Australia
    PLoS ONE 8:e52960. 2013
    ..These probes expand the current palate of lipid order-sensing fluorophores affording greater flexibility in the excitation/emission wavelengths and possibly new opportunities in membrane biology...
  9. pmc Imaging membrane lipid order in whole, living vertebrate organisms
    Dylan M Owen
    Center for Vascular Research, University of New South Wales, Sydney, Australia
    Biophys J 99:L7-9. 2010
    ..The transition of membrane order imaging from cultured cell lines to living organisms is an important step forward in understanding the physiological relevance of membrane microdomains including lipid rafts...
  10. ncbi Quantitative microscopy: protein dynamics and membrane organisation
    Dylan M Owen
    Centre for Vascular Research, University of New South Wales, and the Department of Haematology, Prince of Wales Hospital, Sydney, Australia
    Traffic 10:962-71. 2009
    ..New multi-dimensional microscopy approaches may afford greater temporal and spatial resolution resulting in more accurate quantification of protein and membrane dynamics in live cells...
  11. doi Pre-existing clusters of the adaptor Lat do not participate in early T cell signaling events
    David J Williamson
    Centre for Vascular Research, University of New South Wales, Sydney, Australia
    Nat Immunol 12:655-62. 2011
    ..Our data suggest that TCR ligation preconditions the membrane for vesicle recruitment and bulk activation of the Lat signaling network...
  12. pmc Quantitative analysis of three-dimensional fluorescence localization microscopy data
    Dylan M Owen
    Centre for Vascular Research and Australian Centre for NanoMedicine, University of New South Wales, Sydney, Australia
    Biophys J 105:L05-7. 2013
    ..This approach is particularly useful to map the topography of the plasma membrane and measure protein distribution within an undulating membrane...
  13. doi Conformational states of the kinase Lck regulate clustering in early T cell signaling
    Jérémie Rossy
    Centre for Vascular Research and Australian Centre for NanoMedicine, University of New South Wales, Sydney, Australia
    Nat Immunol 14:82-9. 2013
    ..Our data suggest that Lck conformational states represent an intrinsic mechanism for the intermolecular organization of early T cell signaling...
  14. pmc Dynamic organization of lymphocyte plasma membrane: lessons from advanced imaging methods
    Dylan M Owen
    Centre for Vascular Research, University of New South Wales, Sydney, NSW, Australia
    Immunology 131:1-8. 2010
    ..We provide a comprehensive up-to-date view on the existence of membrane lipid and protein clusters such as lipid rafts and suggest research directions to better understand these highly dynamic entities on the surface of immune cells...
  15. ncbi Method for co-cluster analysis in multichannel single-molecule localisation data
    Jérémie Rossy
    Centre for Vascular Research and Australian Centre for NanoMedicine, University of New South Wales, Sydney, Australia
    Histochem Cell Biol 141:605-12. 2014
    ..Analysing co-clustering in this manner is generalizable to higher numbers of fluorescent species and to three-dimensional or live cell data sets. ..
  16. pmc HIV taken by STORM: super-resolution fluorescence microscopy of a viral infection
    Candida F Pereira
    Centre for Virology, Burnet Institute, Melbourne, Australia
    Virol J 9:84. 2012
    ....
  17. pmc Protein kinase C controls vesicular transport and secretion of apolipoprotein E from primary human macrophages
    Denuja Karunakaran
    Centre for Vascular Research, School of Medical Sciences, University of New South Wales, Sydney, Australia
    J Biol Chem 288:5186-97. 2013
    ..In conclusion, PKC regulates the secretion of apoE from primary human macrophages...