Research Topics
Genomes and Genes
| Brett NixonSummaryAffiliation: University of Newcastle Country: Australia Publications
| Collaborators
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Detail Information
Publications
The biological significance of detergent-resistant membranes in spermatozoaBrett Nixon
Reproductive Science Group, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia
J Reprod Immunol 83:8-13. 2009....
Monotremes provide a key to understanding the evolutionary significance of epididymal sperm maturationBrett Nixon
School of Environmental and Life Sciences, Discipline of Biological Sciences, University of Newcastle, University Drive, Callaghan, NSW 2308, Australia
J Androl 32:665-71. 2011....
Composition and significance of detergent resistant membranes in mouse spermatozoaBrett Nixon
Reproductive Science Group, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales, Australia
J Cell Physiol 218:122-34. 2009....
Proteomic and functional analysis of human sperm detergent resistant membranesBrett Nixon
Reproductive Science Group, University of Newcastle, Callaghan, New South Wales, Australia
J Cell Physiol 226:2651-65. 2011..Collectively these data afford novel insights into the subcellular localization and potential functions of membrane rafts in human spermatozoa...
Molecular and functional characterization of the rabbit epididymal secretory protein 52, REP52Brett Nixon
School of Environmental and Life Sciences, Discipline of Biological Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
Biol Reprod 78:910-20. 2008....
Elucidation of the signaling pathways that underpin capacitation-associated surface phosphotyrosine expression in mouse spermatozoaBrett Nixon
Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, Australia
J Cell Physiol 224:71-83. 2010..Collectively, these data highlight a previously unappreciated role of the ERK module in the modification of the sperm surface during capacitation to render these cells functionally competent to engage in the process of fertilization...
Identification of the molecular chaperone, heat shock protein 1 (chaperonin 10), in the reproductive tract and in capacitating spermatozoa in the male mouseAndrew Walsh
Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
Biol Reprod 78:983-93. 2008..Collectively, these data invite speculation that a number of molecular chaperones are involved in modification of the sperm surface during capacitation to render these cells functionally competent to engage the process of fertilization...
Investigation of the role of SRC in capacitation-associated tyrosine phosphorylation of human spermatozoaLisa A Mitchell
Reproductive Science Group, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
Mol Hum Reprod 14:235-43. 2008..However, although the inhibition of PKA was also accompanied by a suppression of sperm motility, SRC inhibition did not induce a similar response...
Involvement of multimeric protein complexes in mediating the capacitation-dependent binding of human spermatozoa to homologous zonae pellucidaeKate A Redgrove
Reproductive Science Group, School of Environmental and Life Sciences, Discipline of Biological Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
Dev Biol 356:460-74. 2011..Overall, the strategies employed in this study have provided novel, causal insights into the molecular mechanisms that govern sperm-egg interaction...
Dynamin regulates specific membrane fusion events necessary for acrosomal exocytosis in mouse spermatozoaAndrew T Reid
School of Environmental and Life Sciences, Discipline of Biological Sciences, The University of Newcastle, University Drive, Callaghan, New South Wales 2308, Australia
J Biol Chem 287:37659-72. 2012..On the basis of our collective data, we propose that dynamin could regulate specific membrane fusion events necessary for acrosomal exocytosis in mouse spermatozoa...
The identification of mouse sperm-surface-associated proteins and characterization of their ability to act as decapacitation factorsBrett Nixon
Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
Biol Reprod 74:275-87. 2006..Of these proteins, PBP was identified as a primary candidate for a decapacitation factor...
The chaperonin containing TCP1 complex (CCT/TRiC) is involved in mediating sperm-oocyte interactionMatthew D Dun
Reproductive Science Group, School of Environmental and Life Sciences, Discipline of Biological Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
J Biol Chem 286:36875-87. 2011..Collectively, these data provide an important biochemical insight into the molecular basis of sperm-zona pellucida interaction and a plausible explanation for how spermatozoa gain their ability to fertilize...
Localization and significance of molecular chaperones, heat shock protein 1, and tumor rejection antigen gp96 in the male reproductive tract and during capacitation and acrosome reactionKelly L Asquith
Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
Biol Reprod 72:328-37. 2005..These data reinforce the notion that these chaperones are intimately involved in the mechanisms by which mammalian spermatozoa both acquire and express their ability to recognize the zona pellucida...
Proteomic insights into the maturation and capacitation of mammalian spermatozoaMark A Baker
PRC in Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, NSW, Australia
Syst Biol Reprod Med 58:211-7. 2012..As a result of such advances, insights will be generated that should ultimately lead to significant improvements in our capacity to diagnose and treat the infertile male...
Tyrosine phosphorylation activates surface chaperones facilitating sperm-zona recognitionKelly L Asquith
Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, University Drive, Callaghan, NSW 2308, Australia
J Cell Sci 117:3645-57. 2004....
Chronic exposure to acrylamide induces DNA damage in male germ cells of miceBelinda J Nixon
Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
Toxicol Sci 129:135-45. 2012..01 µg/ml (0.001 mg/kg bodyweight/day). The results of this study are the first to demonstrate that chronic exposure to acrylamide, at doses equivalent to human exposures, generates DNA damage in male germ cells of mice...
Role of the epididymis in sperm competitionRussell C Jones
Discipline of Biological Sciences, The University of Newcastle, Callaghan 2308, Australia
Asian J Androl 9:493-9. 2007....
Proteomic changes in mammalian spermatozoa during epididymal maturationR John Aitken
The ARC Centre of Excellence in Biotechnology and Development, University of Newcastle, Newcastle, NSW 2308, Australia
Asian J Androl 9:554-64. 2007..At least in rodent species, activation of sperm mitochondria appears to be a particularly important part of epididymal maturation...
Evidence for the involvement of PECAM-1 in a receptor mediated signal-transduction pathway regulating capacitation-associated tyrosine phosphorylation in human spermatozoaBrett Nixon
Reproductive Science Group, University of Newcastle, Callaghan, NSW 2308, Australia
J Cell Sci 118:4865-77. 2005..Collectively, these data provide the first evidence that a receptor-mediated signal transduction pathway triggers human sperm capacitation and identifies PECAM-1 as the probable initiator of this second messenger cascade...
Investigation of the mechanisms by which the molecular chaperone HSPA2 regulates the expression of sperm surface receptors involved in human sperm-oocyte recognitionKate A Redgrove
Reproductive Science Group, School of Environmental and Life Sciences, Discipline of Biological Sciences, University of Newcastle, University Drive, Callaghan, NSW 2308, Australia
Mol Hum Reprod 19:120-35. 2013..Collectively these data afford novel insights into the sub-cellular localization and potential functions of multimeric protein complexes in human spermatozoa...
A unique combination of male germ cell miRNAs coordinates gonocyte differentiationSkye C McIver
ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales, Australia
PLoS ONE 7:e35553. 2012..These targets converge on the key downstream cell cycle regulator Cyclin D1, indicating that a unique combination of male germ cell miRNAs coordinate the differentiation and maintenance of pluripotency in germ cells...
Cellular mechanisms regulating sperm-zona pellucida interactionAndrew T Reid
Reproductive Science Group, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
Asian J Androl 13:88-96. 2011....
Staying alive: PI3K pathway promotes primordial follicle activation and survival in response to 3MC-induced ovotoxicityAlexander P Sobinoff
Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
Toxicol Sci 128:258-71. 2012....
Understanding the Villain: DMBA-induced preantral ovotoxicity involves selective follicular destruction and primordial follicle activation through PI3K/Akt and mTOR signalingAlexander P Sobinoff
Reproductive Science Group, School of Environmental and Life Sciences
Toxicol Sci 123:563-75. 2011..Our results reveal a novel mechanism of DMBA-induced preantral ovotoxicity involving selective immature follicle destruction and primordial follicle activation involving downstream members of the PI3K/Akt and mTOR signaling pathways...
Identification of RARhoGAP, a novel putative RhoGAP gene expressed in male germ cellsBenjamin J Curry
Reproductive Science Group, Discipline of Biological Science, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
Genomics 84:406-18. 2004..We postulate that RARhoGAP may be involved in rearrangements of the cytoskeleton and cell signaling events that occur during spermatogenesis...
Rabbit epididymal secretory proteins. I. Characterization and hormonal regulationBrett Nixon
Department of Biological Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
Biol Reprod 67:133-9. 2002..Seven of the proteins gained were about the same molecular weight as proteins secreted by the epididymis (M(r) 94 000, 52 000, 38 000, 36 000, 22 000, 20 000, and 13 000) and were analyzed using N-terminal amino acid microsequencing...
The role of molecular chaperones in mouse sperm-egg interactionsBrett Nixon
Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia
Mol Cell Endocrinol 240:1-10. 2005....
DNA damage in human spermatozoa is highly correlated with the efficiency of chromatin remodeling and the formation of 8-hydroxy-2'-deoxyguanosine, a marker of oxidative stressGeoffry N De Iuliis
ARC Centre of Excellence in Biotechnology and Development and Hunter Medical Research Institute, Discipline of Biological Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
Biol Reprod 81:517-24. 2009..These results clearly highlight the importance of oxidative stress in the induction of sperm DNA damage and carry significant implications for the clinical management of this condition...
The molecular chaperone HSPA2 plays a key role in regulating the expression of sperm surface receptors that mediate sperm-egg recognitionKate A Redgrove
Priority Research Centre in Reproductive Science, School of Environmental and Life Sciences, Discipline of Biological Sciences, The University of Newcastle, Callaghan, New South Wales, Australia
PLoS ONE 7:e50851. 2012....
Adding insult to injury: effects of xenobiotic-induced preantral ovotoxicity on ovarian development and oocyte fusibilityAlexander P Sobinoff
Reproductive Science Group, Callaghan, New South Wales 2308, Australia
Toxicol Sci 118:653-66. 2010..5-15 mg/kg/day) neonatal exposure to xenobiotics causing long-term reactive oxygen species-induced oocyte dysfunction...
The role of molecular chaperones in spermatogenesis and the post-testicular maturation of mammalian spermatozoaMatthew D Dun
School of Environmental and Life Sciences, Discipline of Biological Sciences, University of Newcastle, University Drive, Callaghan, NSW 2308, Australia
Hum Reprod Update 18:420-35. 2012....
Rabbit epididymal secretory proteins. III. Molecular cloning and characterization of the complementary DNA for REP38Brett Nixon
Pest Animal Control Cooperative Research Centre, CSIRO Sustainable Ecosystems, Canberra, ACT 2601, Australia
Biol Reprod 67:147-53. 2002..Although REP38-c1 mRNA was detected only in the rabbit epididymis, database searches indicated homology with two rat testis specific cDNAs, KTT4 and odf2, which encode sperm outer dense fiber proteins...
Rabbit epididymal secretory proteins. II. Immunolocalization and sperm association of REP38Brett Nixon
Pest Animal Control Cooperative Research Centre, CSIRO Sustainable Ecosystems, Canberra, ACT 2601, Australia
Biol Reprod 67:140-6. 2002..When tested in vitro, anti-REP38 IgG reduced the percentage of ova fertilized in a concentration-dependent manner, apparently by blocking sperm-egg fusion...
Genome analysis of the platypus reveals unique signatures of evolutionWesley C Warren
Genome Sequencing Center, Washington University School of Medicine, Campus Box 8501, 4444 Forest Park Avenue, St Louis, Missouri 63108, USA
Nature 453:175-83. 2008..Sequencing of this genome now provides a valuable resource for deep mammalian comparative analyses, as well as for monotreme biology and conservation...
