Karen L MacKenzie

Summary

Affiliation: University of New South Wales
Country: Australia

Publications

  1. pmc Modeling human endothelial cell transformation in vascular neoplasias
    Victoria W Wen
    Cancer Cell Development Group, Children s Cancer Institute Australia for Medical Research, Lowy Cancer Research Centre, University of New South Wales, Randwick, NSW, Australia
    Dis Model Mech 6:1066-79. 2013
  2. pmc Endothelial cell dysfunction and cytoskeletal changes associated with repression of p16(INK4a) during immortalization
    C Y Kan
    Cancer Cell Development Group, Children s Cancer Institute Australia, Lowy Cancer Research Centre, University of New South Wales, Randwick, NSW, Australia
    Oncogene 31:4815-27. 2012
  3. ncbi request reprint Multiple stages of malignant transformation of human endothelial cells modelled by co-expression of telomerase reverse transcriptase, SV40 T antigen and oncogenic N-ras
    Karen L MacKenzie
    James Ewing Laboratory of Developmental Haematopoiesis and Department of Human Genetics, Sloan Kettering Cancer Institute, New York, NY, USA
    Oncogene 21:4200-11. 2002
  4. ncbi request reprint Osteopetrotic mouse stroma with thrombopoietin, c-kit ligand, and flk-2 ligand supports long-term mobilized CD34+ hematopoiesis in vitro
    Pierre Feugier
    James Ewing Laboratory of Developmental Hematopoiesis, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Stem Cells Dev 14:505-16. 2005
  5. doi request reprint Mild hyperoxia limits hTR levels, telomerase activity, and telomere length maintenance in hTERT-transduced bone marrow endothelial cells
    Christine E Napier
    Lowy Cancer Research Centre, University of New South Wales, Randwick, NSW, Australia
    Biochim Biophys Acta 1803:1142-53. 2010
  6. ncbi request reprint Methotrexate selection of long-term culture initiating cells following transduction of CD34(+) cells with a retrovirus containing a mutated human dihydrofolate reductase gene
    Naoko Takebe
    Program of Molecular Pharmacology and Experimental Therapeutics, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Cancer Gene Ther 9:308-20. 2002
  7. ncbi request reprint Ex vivo expansion of stem and progenitor cells in co-culture of mobilized peripheral blood CD34+ cells on human endothelium transfected with adenovectors expressing thrombopoietin, c-kit ligand, and Flt-3 ligand
    Pierre Feugier
    James Ewing Laboratory of Developmental Hematopoiesis, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, Box 101, New York, NY 10021, USA
    J Hematother Stem Cell Res 11:127-38. 2002
  8. ncbi request reprint Telomere-driven karyotypic complexity concurs with p16INK4a inactivation in TP53-competent immortal endothelial cells
    Victoria W Wen
    Children s Cancer Institute Australia for Medical Research, Randwick, New South Wales, Australia
    Cancer Res 66:10691-700. 2006
  9. ncbi request reprint Limited proliferation and telomere dysfunction following telomerase inhibition in immortal murine fibroblasts
    Jessica Boklan
    James Ewing Laboratory of Developmental Hematopoiesis, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Cancer Res 62:2104-14. 2002
  10. ncbi request reprint Dexamethasone resistance in B-cell precursor childhood acute lymphoblastic leukemia occurs downstream of ligand-induced nuclear translocation of the glucocorticoid receptor
    Petra S Bachmann
    Children s Cancer Institute Australia for Medical Research, PO Box 81, High Street, Randwick, NSW 2031, Australia
    Blood 105:2519-26. 2005

Collaborators

Detail Information

Publications25

  1. pmc Modeling human endothelial cell transformation in vascular neoplasias
    Victoria W Wen
    Cancer Cell Development Group, Children s Cancer Institute Australia for Medical Research, Lowy Cancer Research Centre, University of New South Wales, Randwick, NSW, Australia
    Dis Model Mech 6:1066-79. 2013
    ..Human EC lines, established through the introduction of defined genetic elements or by culture of primary tumor tissue, are catalogued and discussed in relation to their relevance as models of vascular neoplasia. ..
  2. pmc Endothelial cell dysfunction and cytoskeletal changes associated with repression of p16(INK4a) during immortalization
    C Y Kan
    Cancer Cell Development Group, Children s Cancer Institute Australia, Lowy Cancer Research Centre, University of New South Wales, Randwick, NSW, Australia
    Oncogene 31:4815-27. 2012
    ..These defects are likely to reflect alterations that occur during the development of EC-derived malignancies...
  3. ncbi request reprint Multiple stages of malignant transformation of human endothelial cells modelled by co-expression of telomerase reverse transcriptase, SV40 T antigen and oncogenic N-ras
    Karen L MacKenzie
    James Ewing Laboratory of Developmental Haematopoiesis and Department of Human Genetics, Sloan Kettering Cancer Institute, New York, NY, USA
    Oncogene 21:4200-11. 2002
    ..This model will be useful for understanding mechanisms underlying vascular and angiogenic neoplasias, as well as for testing drugs designed to curtail aberrant EC growth...
  4. ncbi request reprint Osteopetrotic mouse stroma with thrombopoietin, c-kit ligand, and flk-2 ligand supports long-term mobilized CD34+ hematopoiesis in vitro
    Pierre Feugier
    James Ewing Laboratory of Developmental Hematopoiesis, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Stem Cells Dev 14:505-16. 2005
    ....
  5. doi request reprint Mild hyperoxia limits hTR levels, telomerase activity, and telomere length maintenance in hTERT-transduced bone marrow endothelial cells
    Christine E Napier
    Lowy Cancer Research Centre, University of New South Wales, Randwick, NSW, Australia
    Biochim Biophys Acta 1803:1142-53. 2010
    ..Overall, these results demonstrate that hTR levels are reduced by mild hyperoxia and limit telomerase-mediated telomere lengthening in hTERT-transduced ECs...
  6. ncbi request reprint Methotrexate selection of long-term culture initiating cells following transduction of CD34(+) cells with a retrovirus containing a mutated human dihydrofolate reductase gene
    Naoko Takebe
    Program of Molecular Pharmacology and Experimental Therapeutics, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Cancer Gene Ther 9:308-20. 2002
    ..These are the first investigations to demonstrate selection for transduced long-term culture initiating cells using MTX. The DHFR/MTX system holds promise for improving selection of gene-transduced hematopoietic progenitor cells in vivo...
  7. ncbi request reprint Ex vivo expansion of stem and progenitor cells in co-culture of mobilized peripheral blood CD34+ cells on human endothelium transfected with adenovectors expressing thrombopoietin, c-kit ligand, and Flt-3 ligand
    Pierre Feugier
    James Ewing Laboratory of Developmental Hematopoiesis, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, Box 101, New York, NY 10021, USA
    J Hematother Stem Cell Res 11:127-38. 2002
    ....
  8. ncbi request reprint Telomere-driven karyotypic complexity concurs with p16INK4a inactivation in TP53-competent immortal endothelial cells
    Victoria W Wen
    Children s Cancer Institute Australia for Medical Research, Randwick, New South Wales, Australia
    Cancer Res 66:10691-700. 2006
    ..These investigations provide new insight to the role of p16(INK4a) as a tumor suppressor...
  9. ncbi request reprint Limited proliferation and telomere dysfunction following telomerase inhibition in immortal murine fibroblasts
    Jessica Boklan
    James Ewing Laboratory of Developmental Hematopoiesis, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Cancer Res 62:2104-14. 2002
    ..Moreover, the murine model used here should prove useful in further evaluating telomerase inhibition as an anticancer therapy...
  10. ncbi request reprint Dexamethasone resistance in B-cell precursor childhood acute lymphoblastic leukemia occurs downstream of ligand-induced nuclear translocation of the glucocorticoid receptor
    Petra S Bachmann
    Children s Cancer Institute Australia for Medical Research, PO Box 81, High Street, Randwick, NSW 2031, Australia
    Blood 105:2519-26. 2005
    ..These results show that dexamethasone resistance in BCP ALL xenografts occurs downstream of ligand-induced nuclear translocation of the GR, but upstream of Bim induction...
  11. ncbi request reprint A role for altered microtubule polymer levels in vincristine resistance of childhood acute lymphoblastic leukemia xenografts
    Vivienna Ong
    Children s Cancer Institute Australia, PO Box 81, High St, Randwick 2031, NSW, Australia
    J Pharmacol Exp Ther 324:434-42. 2008
    ..These results indicate that the balance between polymerized and nonpolymerized tubulin may be an important determinant of response to Vinca alkaloid-based chemotherapy regimens in childhood ALL...
  12. ncbi request reprint Inactivation of p16INK4a, with retention of pRB and p53/p21cip1 function, in human MRC5 fibroblasts that overcome a telomere-independent crisis during immortalization
    Lisa M Taylor
    Children s Cancer Institute Australia for Medical Research, Randwick, New South Wales 2031
    J Biol Chem 279:43634-45. 2004
    ..Overall, these results implicate the inactivation of p16INK4a as a critical event for overcoming telomere-independent crisis, immortalizing MRC5 fibroblasts and overcoming ras-induced premature senescence...
  13. ncbi request reprint Mutant N-ras preferentially drives human CD34+ hematopoietic progenitor cells into myeloid differentiation and proliferation both in vitro and in the NOD/SCID mouse
    Sylvie W Shen
    Children s Cancer Institute Australia for Medical Research, Randwick, NSW, Australia
    Exp Hematol 32:852-60. 2004
    ..In the present study we investigated the effect of N-rasm in human primary hematopoietic progenitor cells (HPC)...
  14. doi request reprint Induction of vascular endothelial growth factor secretion by childhood acute lymphoblastic leukemia cells via the FLT-3 signaling pathway
    Ana Markovic
    Children s Cancer Institute Australia for Medical Research, Lowy Cancer Research Centre, University of New South Wales, Sydney, New South Wales, Australia
    Mol Cancer Ther 11:183-93. 2012
    ....
  15. pmc p53-independent epigenetic repression of the p21(WAF1) gene in T-cell acute lymphoblastic leukemia
    Carwyn Davies
    Children s Cancer Institute Australia for Medical Research, Lowy Cancer Research Centre, University of New South Wales, Sydney, New South Wales 2052, Australia
    J Biol Chem 286:37639-50. 2011
    ..Considering the known anti-apoptotic function of p21(WAF1), our findings have significant implications for the responses of T- versus BCP-ALL cells to chemotherapeutic drugs that induce p21(WAF1)...
  16. pmc Up-regulation of survivin during immortalization of human myofibroblasts is linked to repression of tumor suppressor p16(INK4a) protein and confers resistance to oxidative stress
    Chin Yi Kan
    Cancer Cell Development Group, Children s Cancer Institute Australia, Lowy Cancer Research Centre, University of New South Wales, Randwick, New South Wales 2031, Australia
    J Biol Chem 288:12032-41. 2013
    ..The up-regulation of survivin during immortalization likely contributes to the vulnerability of immortal cells to transformation by oncogenes that alter intracellular redox state...
  17. pmc Concentration- and schedule-dependent effects of chemotherapy on the angiogenic potential and drug sensitivity of vascular endothelial cells
    Eddy Pasquier
    Children s Cancer Institute Australia for Medical Research, Lowy Cancer Research Centre, UNSW, PO Box 81, Randwick, NSW, 2031, Australia
    Angiogenesis 16:373-86. 2013
    ..Furthermore, our study suggests that ╬▓III-tubulin represents an attractive therapeutic target to increase the anti-angiogenic effects of chemotherapy and overall anti-tumour efficacy...
  18. doi request reprint Assessment and quantification of telomerase enzyme activity
    Michelle F Maritz
    Cancer Cell Development Group, Children s Cancer Institute Australia, Randwick, Australia
    Methods Mol Biol 965:215-31. 2013
    ..The Q-TRAP method poses the additional advantages of exquisite sensitivity, rapidity, and potential for adoption to a high-throughput format...
  19. ncbi request reprint p53 determines multidrug sensitivity of childhood neuroblastoma
    Chengyuan Xue
    Children s Cancer Institute Australia for Medical Research, Randwick, New South Wales, Australia
    Cancer Res 67:10351-60. 2007
    ....
  20. pmc SIRT1 promotes N-Myc oncogenesis through a positive feedback loop involving the effects of MKP3 and ERK on N-Myc protein stability
    Glenn M Marshall
    Children s Cancer Institute Australia for Medical Research, Randwick, Australia
    PLoS Genet 7:e1002135. 2011
    ..Our data demonstrate the important roles of SIRT1 in N-Myc oncogenesis and SIRT1 inhibitors in the prevention and therapy of N-Myc-induced neuroblastoma...
  21. doi request reprint Targeting telomerase in hematologic malignancy
    Michelle F Maritz
    Children s Cancer Institute Australia for Medical Research, Lowy Cancer Research Centre, New South Wales, Australia
    Future Oncol 6:769-89. 2010
    ....
  22. ncbi request reprint Characterization of childhood acute lymphoblastic leukemia xenograft models for the preclinical evaluation of new therapies
    Natalia L M Liem
    Children s Cancer Institute Australia for Medical Research, Sydney, Australia
    Blood 103:3905-14. 2004
    ..As such, they provide powerful experimental models to prioritize new therapeutic strategies for future clinical trials...
  23. ncbi request reprint The AML1-ETO fusion protein promotes the expansion of human hematopoietic stem cells
    James C Mulloy
    Laboratory of Molecular Hematopoiesis, Sloan Kettering Institute, New York, NY, USA
    Blood 99:15-23. 2002
    ..Thus, AML1-ETO enhances the self-renewal of pluripotent stem cells, the physiological target of many acute myeloid leukemias...
  24. ncbi request reprint FLT-3: a new focus in the understanding of acute leukemia
    Ana Markovic
    Children s Cancer Institute Australia for Medical Research, PO Box 81, High Street, Randwick, NSW 2031, Australia
    Int J Biochem Cell Biol 37:1168-72. 2005
    ....
  25. doi request reprint ERG promotes T-acute lymphoblastic leukemia and is transcriptionally regulated in leukemic cells by a stem cell enhancer
    Julie A I Thoms
    Lowy Cancer Research Centre and the Prince of Wales Clinical School, University of New South Wales, Sydney, Australia
    Blood 117:7079-89. 2011
    ..Taken together, these data illustrate that ERG promotes T-ALL and that failure to extinguish activity of stem cell enhancers associated with regulatory transcription factors such as ERG can contribute to the development of leukemia...