I M Mackay

Summary

Affiliation: University of Queensland
Country: Australia

Publications

  1. ncbi request reprint Quantitative PCR-ELAHA for the determination of retroviral vector transduction efficiency
    I M Mackay
    Gene Therapy Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Herston Road, Herston, Queensland 4029, Australia
    Mol Ther 3:801-8. 2001
  2. pmc Real-time PCR in virology
    Ian M Mackay
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Brisbane, Australia
    Nucleic Acids Res 30:1292-305. 2002
  3. doi request reprint Molecular characterization and distinguishing features of a novel human rhinovirus (HRV) C, HRVC-QCE, detected in children with fever, cough and wheeze during 2003
    K E Arden
    Sir Albert Sakzewski Virus Research Centre, Queensland Children s Medical Research Institute, Royal Children s Hospital, Queensland, Australia
    J Clin Virol 47:219-23. 2010
  4. doi request reprint A novel duplex real-time PCR for HPIV-4 detects co-circulation of both viral subtypes among ill children during 2008
    C Y T Wang
    Queensland Paediatric Infectious Diseases Laboratory, Queensland Children s Medical Research Institute, Sir Albert Sakzewski Virus Research Centre, Children s Health Services District, University of Queensland, Australia
    J Clin Virol 54:83-5. 2012
  5. pmc Detection and differentiation of human polyomaviruses JC and BK by LightCycler PCR
    D M Whiley
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital and Health Service District, Queensland, Australia
    J Clin Microbiol 39:4357-61. 2001
  6. ncbi request reprint Real-time PCR in the microbiology laboratory
    I M Mackay
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre and Department of Paediatrics, Royal Children s Hospital, Brisbane, Queensland, Australia
    Clin Microbiol Infect 10:190-212. 2004
  7. ncbi request reprint Preliminary comparison of three LightCycler PCR assays for the detection of herpes simplex virus in swab specimens
    D M Whiley
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Herston Road, 4029 Herston, Queensland, Australia
    Eur J Clin Microbiol Infect Dis 22:764-7. 2003
  8. ncbi request reprint Characterisation of a newly identified human rhinovirus, HRV-QPM, discovered in infants with bronchiolitis
    P McErlean
    Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Queensland, Australia
    J Clin Virol 39:67-75. 2007
  9. ncbi request reprint Evidence of human metapneumovirus in Australian children
    Michael D Nissen
    Med J Aust 176:188. 2002
  10. ncbi request reprint Evidence of human coronavirus HKU1 and human bocavirus in Australian children
    Theo P Sloots
    Queensland Paediatric Infectious Diseases Laboratory, CVRU, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Herston Road, Herston, Queensland 4029, Australia
    J Clin Virol 35:99-102. 2006

Collaborators

Detail Information

Publications38

  1. ncbi request reprint Quantitative PCR-ELAHA for the determination of retroviral vector transduction efficiency
    I M Mackay
    Gene Therapy Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Herston Road, Herston, Queensland 4029, Australia
    Mol Ther 3:801-8. 2001
    ..The results revealed the number of copies of retrovector provirus present per stably transduced cell using vectors containing either one or both qPCR targets...
  2. pmc Real-time PCR in virology
    Ian M Mackay
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Brisbane, Australia
    Nucleic Acids Res 30:1292-305. 2002
    ..However, the technology discussed has been applied to other areas of microbiology as well as studies of gene expression and genetic disease...
  3. doi request reprint Molecular characterization and distinguishing features of a novel human rhinovirus (HRV) C, HRVC-QCE, detected in children with fever, cough and wheeze during 2003
    K E Arden
    Sir Albert Sakzewski Virus Research Centre, Queensland Children s Medical Research Institute, Royal Children s Hospital, Queensland, Australia
    J Clin Virol 47:219-23. 2010
    ....
  4. doi request reprint A novel duplex real-time PCR for HPIV-4 detects co-circulation of both viral subtypes among ill children during 2008
    C Y T Wang
    Queensland Paediatric Infectious Diseases Laboratory, Queensland Children s Medical Research Institute, Sir Albert Sakzewski Virus Research Centre, Children s Health Services District, University of Queensland, Australia
    J Clin Virol 54:83-5. 2012
    ..Our assay proved suitably sensitive and specific for inclusion in future studies seeking to better understand the role HPIV-4 and other respiratory viruses in children with ARI...
  5. pmc Detection and differentiation of human polyomaviruses JC and BK by LightCycler PCR
    D M Whiley
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital and Health Service District, Queensland, Australia
    J Clin Microbiol 39:4357-61. 2001
    ..These data, combined with its rapid turnaround time for results and decreased hands-on time, make the LightCycler PCR assay highly suitable for the rapid detection and differentiation of JCV and BKV in the clinical laboratory...
  6. ncbi request reprint Real-time PCR in the microbiology laboratory
    I M Mackay
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre and Department of Paediatrics, Royal Children s Hospital, Brisbane, Queensland, Australia
    Clin Microbiol Infect 10:190-212. 2004
    ..Additionally, the advantages, limitations and general background of real-time PCR technology will be reviewed in the context of the microbiology laboratory...
  7. ncbi request reprint Preliminary comparison of three LightCycler PCR assays for the detection of herpes simplex virus in swab specimens
    D M Whiley
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Herston Road, 4029 Herston, Queensland, Australia
    Eur J Clin Microbiol Infect Dis 22:764-7. 2003
    ..However, differentiation of herpes simplex virus types 1 and 2 by melting curve analysis was problematic in all assays. Overall, the results highlight the limitations of typing herpes simplex virus by melting curve analysis...
  8. ncbi request reprint Characterisation of a newly identified human rhinovirus, HRV-QPM, discovered in infants with bronchiolitis
    P McErlean
    Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Queensland, Australia
    J Clin Virol 39:67-75. 2007
    ....
  9. ncbi request reprint Evidence of human metapneumovirus in Australian children
    Michael D Nissen
    Med J Aust 176:188. 2002
  10. ncbi request reprint Evidence of human coronavirus HKU1 and human bocavirus in Australian children
    Theo P Sloots
    Queensland Paediatric Infectious Diseases Laboratory, CVRU, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Herston Road, Herston, Queensland 4029, Australia
    J Clin Virol 35:99-102. 2006
    ..Children under the age of 2 years were most at risk of infection by these viruses which contribute significantly to the microbial burden among patients with respiratory tract disease during the colder months...
  11. ncbi request reprint Detection and discrimination of herpes simplex viruses, Haemophilus ducreyi, Treponema pallidum, and Calymmatobacterium (Klebsiella) granulomatis from genital ulcers
    Ian M Mackay
    Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Queensland, Australia
    Clin Infect Dis 42:1431-8. 2006
    ..This collaborative project was undertaken to address the need for a rapid, economical, and sensitive approach to the detection and diagnosis of GUD using noninvasive techniques to sample genital ulcers...
  12. ncbi request reprint Genetic diversity of human metapneumovirus over 4 consecutive years in Australia
    Ian M Mackay
    Queensland Paediatric Infectious Disease Laboratory, Royal Children s Hospital, University of Queensland, Queensland, Australia
    J Infect Dis 193:1630-3. 2006
    ..Concurrent, annual circulation of all 4 hMPV subtypes in our study population was common, with a single, usually different hMPV subtype predominating in each year...
  13. ncbi request reprint Frequent detection of human rhinoviruses, paramyxoviruses, coronaviruses, and bocavirus during acute respiratory tract infections
    Katherine E Arden
    Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Queensland, Australia
    J Med Virol 78:1232-40. 2006
    ....
  14. pmc Human metapneumovirus, Australia, 2001-2004
    Theo P Sloots
    Queensland Paediatric Infectious Diseases Laboratory, Royal Children s Hospital and Health Service District, Herston Road, Herston, Queensland 4029, Australia
    Emerg Infect Dis 12:1263-6. 2006
    ..1% average annual incidence of human metapneumovirus. The epidemic peak of infection was late winter to spring, and genotyping showed a change in predominant viral genotype in 3 of the 4 years...
  15. pmc Identification of a novel polyomavirus from patients with acute respiratory tract infections
    Anne M Gaynor
    Department of Molecular Microbiology, Washington University School of Medicine, St Louis, Missouri, United States of America
    PLoS Pathog 3:e64. 2007
    ..The presence of multiple instances of the virus in two continents suggests that this virus is geographically widespread in the human population and raises the possibility that the WU virus may be a human pathogen...
  16. ncbi request reprint Community epidemiology of human metapneumovirus, human coronavirus NL63, and other respiratory viruses in healthy preschool-aged children using parent-collected specimens
    Stephen B Lambert
    School of Population Health, University of Melbourne, Victoria, Australia
    Pediatrics 120:e929-37. 2007
    ..We also aimed to assess the use of parent collection to obtain an adequate respiratory specimen from acutely unwell children in the community...
  17. doi request reprint Human rhinoviruses: the cold wars resume
    Ian M Mackay
    Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Queensland, Australia
    J Clin Virol 42:297-320. 2008
    ..Early findings from infection studies and use of inefficient detection methods have shaped the way we think of 'common cold' viruses today...
  18. ncbi request reprint A real-time PCR assay for the detection of Neisseria gonorrhoeae by LightCycler
    David M Whiley
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Brisbane, Queensland, Australia
    Diagn Microbiol Infect Dis 42:85-9. 2002
    ..These features combined with the rapid turn-around time for results makes the LC-PCR particularly suitable for the detection of N. gonorrhoeae in a routine clinical laboratory...
  19. ncbi request reprint New human coronavirus, HCoV-NL63, associated with severe lower respiratory tract disease in Australia
    Katherine E Arden
    Clinical Virology and Molecular Microbiology Research Units, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Queensland, Australia
    J Med Virol 75:455-62. 2005
    ..We propose that HCoV-NL63 is a global and seasonal pathogen of both children and adults associated with severe LRT illness...
  20. ncbi request reprint Use of the P gene to genotype human metapneumovirus identifies 4 viral subtypes
    Ian M Mackay
    Clinical Virology and Molecular Microbiology Research Units, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and University of Queensland, Herston Road, Herston, Queensland 4029, Australia
    J Infect Dis 190:1913-8. 2004
    ..Establishing viral identity is essential for the linking of genotype and disease severity...
  21. ncbi request reprint Simultaneous detection and differentiation of human polyomaviruses JC and BK by a rapid and sensitive PCR-ELAHA assay and a survey of the JCV subtypes within an Australian population
    David M Whiley
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Queensland, Australia
    J Med Virol 72:467-72. 2004
    ..The results of this study show that the VP1-PCR-ELAHA is a sensitive, specific and rapid method for detecting and differentiating human polyomaviruses JC and BK and is highly suitable for routine use in the clinical laboratory...
  22. ncbi request reprint Co-detection and discrimination of six human herpesviruses by multiplex PCR-ELAHA
    Ian M Mackay
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Brisbane, Australia
    J Clin Virol 28:291-302. 2003
    ..Multiplex PCR (mPCR) is capable of simultaneously amplifying a range of targets from a single preparation of nucleic acids and when combined with a suitable detection assay, it is capable of discriminating each of the amplicons...
  23. ncbi request reprint Detection and differentiation of herpes simplex virus types 1 and 2 by a duplex LightCycler PCR that incorporates an internal control PCR reaction
    David M Whiley
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Herston Road, Herston, Queensland 4029, Australia
    J Clin Virol 30:32-8. 2004
    ..The advent of real-time HSV PCR protocols now enables rapid result turnaround times with minimal hands-on time...
  24. pmc A sensitive, specific, and cost-effective multiplex reverse transcriptase-PCR assay for the detection of seven common respiratory viruses in respiratory samples
    Melanie W Syrmis
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Queensland, Australia
    J Mol Diagn 6:125-31. 2004
    ....
  25. pmc Molecular assays for detection of human metapneumovirus
    Ian M Mackay
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Brisbane, Queensland, Australia
    J Clin Microbiol 41:100-5. 2003
    ..6%) tested during the comparison of the two diagnostic approaches. We found the real-time RT-PCR to be the test of choice for future investigation of samples for hMPV due to its speed, reproducibility, specificity, and sensitivity...
  26. pmc Detection of human respiratory syncytial virus in respiratory samples by LightCycler reverse transcriptase PCR
    David M Whiley
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Herston, Queensland, Australia
    J Clin Microbiol 40:4418-22. 2002
    ..The sensitivity of LC-RT-PCR was 50 PFU/ml; and this, together with its high specificity and rapid turnaround time, makes the LC-RT-PCR suitable for the detection of hRSV in clinical specimens...
  27. pmc Distinguishing molecular features and clinical characteristics of a putative new rhinovirus species, human rhinovirus C (HRV C)
    Peter McErlean
    Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Brisbane, Queensland, Australia
    PLoS ONE 3:e1847. 2008
    ....
  28. pmc Orthopoxvirus detection in environmental specimens during suspected bioterror attacks: inhibitory influences of common household products
    Andreas Kurth
    Robert Koch Institute, Center for Biological Safety 1, Nordufer 20, 13353 Berlin, Germany
    Appl Environ Microbiol 74:32-7. 2008
    ..Confirmatory methods include virus isolation followed by an agent-specific immunofluorescence assay to confirm the presence of replication-competent particles...
  29. ncbi request reprint Detection of Neisseria meningitidis by LightCycler PCR
    David M Whiley
    Pathology 35:347-9. 2003
  30. pmc Human bocavirus: passenger or pathogen in acute respiratory tract infections?
    Oliver Schildgen
    Institute for Virology, University of Bonn, Bonn, Germany
    Clin Microbiol Rev 21:291-304, table of contents. 2008
    ..The ability to detect primary infection through the development of improved diagnostic methods will be of great importance for future studies seeking to assign a role for HBoV in causing respiratory illnesses...
  31. ncbi request reprint Human bocavirus: multisystem detection raises questions about infection
    Ian M Mackay
    J Infect Dis 196:968-70. 2007
  32. ncbi request reprint Guideline to reference gene selection for quantitative real-time PCR
    Aleksandar Radonic
    Charité Campus Charité Mitte, II Medizinische Klinik mit Schwerpunkt Onkologie und Hämatologie, Humboldt Universitat, Berlin, Germany
    Biochem Biophys Res Commun 313:856-62. 2004
    ..Our results show that "Classical" reference genes are indeed unsuitable, whereas the RNA polymerase II gene was the gene with the most constant expression in different tissues and following stimulation in CCRF-HSB-2 cells...
  33. pmc Real-time PCR assays for detection of bocavirus in human specimens
    Xiaoyan Lu
    Respiratory and Gastroenteritis Viruses Branch, Centers for Disease Control and Prevention, 1600 Clifton Road, N E, Mailstop G04, Atlanta, GA 30333, USA
    J Clin Microbiol 44:3231-5. 2006
    ..5%) were reproducibly positive for HBoV by one or both targets. Our data confirm the possible association of HBoV infection with pneumonia and demonstrate the utility of these real-time PCR assays for HBoV detection...
  34. ncbi request reprint Detection of Neisseria Meningitidis in clinical samples by a duplex real-time PCR targeting the porA and ctrA genes
    David M Whiley
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Herston, and Clinical Medical Virology Centre, University of Queensland, Brisbane, Queensland, Australia
    Mol Diagn 7:141-5. 2003
    ..In recent years PCR has proven to be a highly sensitive and specific method for the diagnosis of infections caused by Neisseria meningitidis...
  35. ncbi request reprint Human coronavirus nomenclature
    Stephen Lambert
    Pediatr Infect Dis J 25:662. 2006
  36. ncbi request reprint Detection and differentiation of Plasmodium species by polymerase chain reaction and colorimetric detection in blood samples of patients with suspected malaria
    David M Whiley
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Clinical Medical Virology Centre, University of Queensland, Brisbane, Queensland, Australia
    Diagn Microbiol Infect Dis 49:25-9. 2004
    ..In summary, the sensitivity, specificity and simplicity of the PCR assay makes it particularly suitable for use in a diagnostic laboratory...
  37. pmc Cytotoxic T-lymphocyte epitope vaccination protects against human metapneumovirus infection and disease in mice
    Karen A Herd
    Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Herston Road, Herston QLD 4029, Australia
    J Virol 80:2034-44. 2006
    ..These results demonstrate for the first time the efficacy of an hMPV CTL epitope vaccine in the control of hMPV infection in a murine model...
  38. ncbi request reprint Specific detection of enterovirus 71 directly from clinical specimens using real-time RT-PCR hybridization probe assay
    Eng Lee Tan
    Department of Microbiology, Faculty of Medicine, National University of Singapore, Singapore
    Mol Cell Probes 20:135-40. 2006
    ..This study showed that the single tube real-time RT-PCR assay developed in this study can be applied as a rapid and sensitive method for specific detection of EV71 directly from clinical specimens...