Joseph W Lynch

Summary

Affiliation: University of Queensland
Country: Australia

Publications

  1. pmc Molecular mechanisms of Cys-loop ion channel receptor modulation by ivermectin
    Timothy Lynagh
    Queensland Brain Institute, The University of Queensland, Brisbane QLD, Australia
    Front Mol Neurosci 5:60. 2012
  2. pmc High Throughput Techniques for Discovering New Glycine Receptor Modulators and their Binding Sites
    Daniel F Gilbert
    Queensland Brain Institute, The University of Queensland Brisbane, QLD, Australia
    Front Mol Neurosci 2:17. 2009
  3. pmc The impact of human hyperekplexia mutations on glycine receptor structure and function
    Anna Bode
    Queensland Brain Institute and School of Biomedical Sciences, The University of Queensland, Queensland 4072, Australia
    Mol Brain 7:2. 2014
  4. ncbi request reprint Glycine receptors: a new therapeutic target in pain pathways
    Joseph W Lynch
    School of Biomedical Sciences, University of Queensland, Brisbane, QLD 4072, Australia
    Curr Opin Investig Drugs 7:48-53. 2006
  5. doi request reprint Native glycine receptor subtypes and their physiological roles
    Joseph W Lynch
    Queensland Brain Institute and School of Biomedical Sciences, University of Queensland, Brisbane QLD 4072, Australia
    Neuropharmacology 56:303-9. 2009
  6. ncbi request reprint Molecular structure and function of the glycine receptor chloride channel
    Joseph W Lynch
    School of Biomedical Sciences, Univ of Queensland, Brisbane QLD 4072, Australia
    Physiol Rev 84:1051-95. 2004
  7. doi request reprint Subunit-specific potentiation of recombinant glycine receptors by NV-31, a bilobalide-derived compound
    Joseph W Lynch
    School of Biomedical Sciences and Queensland Brain Institute, University of Queensland, Brisbane, QLD 4072, Australia
    Neurosci Lett 435:147-51. 2008
  8. doi request reprint Dihydropyridine inhibition of the glycine receptor: subunit selectivity and a molecular determinant of inhibition
    Xuebin Chen
    School of Biomedical Sciences and Queensland Brain Institute, University of Queensland, Brisbane, QLD 4072, Australia
    Neuropharmacology 56:318-27. 2009
  9. ncbi request reprint A picrotoxin-specific conformational change in the glycine receptor M2-M3 loop
    Rebecca Hawthorne
    School of Biomedical Sciences, University of Queensland, Brisbane, Queensland 4072, Australia
    J Biol Chem 280:35836-43. 2005
  10. ncbi request reprint A proposed structural basis for picrotoxinin and picrotin binding in the glycine receptor pore
    Zhe Yang
    School of Biomedical Sciences, University of Queensland, Brisbane, Queensland, Australia
    J Neurochem 103:580-9. 2007

Collaborators

Detail Information

Publications56

  1. pmc Molecular mechanisms of Cys-loop ion channel receptor modulation by ivermectin
    Timothy Lynagh
    Queensland Brain Institute, The University of Queensland, Brisbane QLD, Australia
    Front Mol Neurosci 5:60. 2012
    ..Together, this information provides new insights into the mechanisms of action of this important drug...
  2. pmc High Throughput Techniques for Discovering New Glycine Receptor Modulators and their Binding Sites
    Daniel F Gilbert
    Queensland Brain Institute, The University of Queensland Brisbane, QLD, Australia
    Front Mol Neurosci 2:17. 2009
    ..We also describe a reliable, cost effective method for transfecting HEK293 cells in single wells of a 384-well plate using nanogram quantities of plasmid DNA...
  3. pmc The impact of human hyperekplexia mutations on glycine receptor structure and function
    Anna Bode
    Queensland Brain Institute and School of Biomedical Sciences, The University of Queensland, Queensland 4072, Australia
    Mol Brain 7:2. 2014
    ..Focusing largely on this new information, this review seeks to summarise the effects of mutations on glycine receptor structure and function and how these functional alterations lead to hyperekplexia. ..
  4. ncbi request reprint Glycine receptors: a new therapeutic target in pain pathways
    Joseph W Lynch
    School of Biomedical Sciences, University of Queensland, Brisbane, QLD 4072, Australia
    Curr Opin Investig Drugs 7:48-53. 2006
    ..Future therapies aimed at specifically increasing current flux through alpha3-containing GlyRs may prove effective in providing analgesia...
  5. doi request reprint Native glycine receptor subtypes and their physiological roles
    Joseph W Lynch
    Queensland Brain Institute and School of Biomedical Sciences, University of Queensland, Brisbane QLD 4072, Australia
    Neuropharmacology 56:303-9. 2009
    ..Finally, agents that selectively potentiate different GlyR isoforms may be useful as therapeutic lead compounds for peripheral inflammatory pain and movement disorders such as spasticity...
  6. ncbi request reprint Molecular structure and function of the glycine receptor chloride channel
    Joseph W Lynch
    School of Biomedical Sciences, Univ of Queensland, Brisbane QLD 4072, Australia
    Physiol Rev 84:1051-95. 2004
    ....
  7. doi request reprint Subunit-specific potentiation of recombinant glycine receptors by NV-31, a bilobalide-derived compound
    Joseph W Lynch
    School of Biomedical Sciences and Queensland Brain Institute, University of Queensland, Brisbane, QLD 4072, Australia
    Neurosci Lett 435:147-51. 2008
    ..GlyRs are potential therapeutic targets for chronic anti-inflammatory pain and movement disorders. NV-31, as a positive modulator of these receptors, thus remains viable as a therapeutic candidate for these disorders...
  8. doi request reprint Dihydropyridine inhibition of the glycine receptor: subunit selectivity and a molecular determinant of inhibition
    Xuebin Chen
    School of Biomedical Sciences and Queensland Brain Institute, University of Queensland, Brisbane, QLD 4072, Australia
    Neuropharmacology 56:318-27. 2009
    ..The differential sensitivities of nifedipine and nicardipine for different GlyR isoforms suggest that DHPs may be a useful resource to screen as pharmacological tools for selectively inhibiting different synaptic GlyR isoforms...
  9. ncbi request reprint A picrotoxin-specific conformational change in the glycine receptor M2-M3 loop
    Rebecca Hawthorne
    School of Biomedical Sciences, University of Queensland, Brisbane, Queensland 4072, Australia
    J Biol Chem 280:35836-43. 2005
    ..We thus conclude that the picrotoxin-specific effects on the M2-M3 loop are mediated allosterically. This suggests that the M2-M3 loop responds differently to the occupation of different binding sites...
  10. ncbi request reprint A proposed structural basis for picrotoxinin and picrotin binding in the glycine receptor pore
    Zhe Yang
    School of Biomedical Sciences, University of Queensland, Brisbane, Queensland, Australia
    J Neurochem 103:580-9. 2007
    ....
  11. pmc A cation-pi interaction in the binding site of the glycine receptor is mediated by a phenylalanine residue
    Stephan A Pless
    School of Biomedical Sciences and Queensland Brain Institute, University of Queensland, Brisbane, Queensland 4072, Australia
    J Neurosci 28:10937-42. 2008
    ..The data thus provide an anchor point for locating glycine in its binding site, and demonstrate for the first time a cation-pi interaction between Phe and a neurotransmitter...
  12. pmc Ligand-specific conformational changes in the alpha1 glycine receptor ligand-binding domain
    Stephan A Pless
    Queensland Brain Institute and School of Biomedical Sciences, The University of Queensland, Brisbane, Queensland 4072, Australia
    J Biol Chem 284:15847-56. 2009
    ..These results provide an important step toward mapping the domains crucial for ligand discrimination in the ligand-binding domain of glycine receptors and possibly other Cys loop receptors...
  13. ncbi request reprint Molecular pharmacology of the glycine receptor chloride channel
    Timothy I Webb
    School of Biomedical Sciences, University of Queensland, Brisbane, QLD, Australia
    Curr Pharm Des 13:2350-67. 2007
    ..These issues need to be addressed before GlyR-specific therapeutics can be developed...
  14. ncbi request reprint Tropisetron modulation of the glycine receptor: femtomolar potentiation and a molecular determinant of inhibition
    Zhe Yang
    School of Biomedical Sciences, University of Queensland, Brisbane, QLD, Australia
    J Neurochem 100:758-69. 2007
    ..To our knowledge, tropisetron is the most exquisitely sensitive modulator yet identified for a cys-loop receptor...
  15. doi request reprint Sesterterpene glycinyl-lactams: a new class of glycine receptor modulator from Australian marine sponges of the genus Psammocinia
    Walter Balansa
    Institute for Molecular Bioscience, The University of Queensland, St Lucia, QLD 4072, Australia
    Org Biomol Chem 11:4695-701. 2013
    ..These insights have the potential to inform and inspire the development of new molecular tools to probe GlyR distribution and function, and therapeutics to treat a wide array of GlyR mediated diseases and disorders. ..
  16. doi request reprint Australian marine sponge alkaloids as a new class of glycine-gated chloride channel receptor modulator
    Walter Balansa
    Institute for Molecular Bioscience, The University of Queensland, St Lucia, QLD 4072, Australia
    Bioorg Med Chem 21:4420-5. 2013
    ..Our investigations into the GlyR modulatory properties of 1-9 were further supported by the synthesis of a number of structurally related indole alkaloids...
  17. pmc A cation-π interaction at a phenylalanine residue in the glycine receptor binding site is conserved for different agonists
    Stephan A Pless
    School of Biomedical Sciences, University of Queensland, Brisbane, Queensland, Australia
    Mol Pharmacol 79:742-8. 2011
    ..These data therefore show that similar agonists can have similar but not identical orientations and interactions in the binding pocket and provide a possible explanation for the lower potencies of β-alanine and taurine...
  18. doi request reprint Distinct conformational changes in activated agonist-bound and agonist-free glycine receptor subunits
    Stephan A Pless
    Queensland Brain Institute and School of Biomedical Sciences, The University of Queensland, Brisbane, Australia
    J Neurochem 108:1585-94. 2009
    ....
  19. doi request reprint The M4 transmembrane segment contributes to agonist efficacy differences between alpha1 and alpha3 glycine receptors
    Xuebin Chen
    Queensland Brain Institute and School of Biomedical Sciences, University of Queensland, Brisbane, QLD 4072, Australia
    Mol Membr Biol 26:321-32. 2009
    ..This strong influence of M4 primary structure on partial agonist efficacy suggests that the relatively poorly conserved alpha3 GlyR M4 segment may be a promising domain to target in the search for alpha3 GlyR-specific modulators...
  20. pmc Phosphorylation of α3 glycine receptors induces a conformational change in the glycine-binding site
    Lu Han
    Queensland Brain Institute and School of Biomedical Sciences, The University of Queensland, Brisbane QLD 4072, Australia
    ACS Chem Neurosci 4:1361-70. 2013
    ..More importantly, by demonstrating that phosphorylation alters α3 GlyR glycine-binding site structure, they raise the possibility of developing analgesics that selectively target inflammation-modulated GlyRs. ..
  21. pmc Function of hyperekplexia-causing α1R271Q/L glycine receptors is restored by shifting the affected residue out of the allosteric signalling pathway
    Qiang Shan
    School of Biomedical Sciences, University of Queensland, Brisbane, Queensland, Australia
    Br J Pharmacol 165:2113-23. 2012
    ..Such a strategy makes it possible to design an ideal drug, which only corrects the function of the mutant or modified protein without affecting the WT or naive protein...
  22. ncbi request reprint Asymmetric contribution of alpha and beta subunits to the activation of alphabeta heteromeric glycine receptors
    Qiang Shan
    Department of Physiology and Pharmacology, School of Biomedical Sciences, University of Queensland, Brisbane, Queensland, Australia
    J Neurochem 86:498-507. 2003
    ..In conclusion, this study demonstrates that beta subunits contribute to the activation of the GlyR, but that their involvement in this process is significantly different to that of the alpha subunit...
  23. pmc Activation and desensitization induce distinct conformational changes at the extracellular-transmembrane domain interface of the glycine receptor
    Qian Wang
    Queensland Brain Institute and School of Biomedical Sciences, University of Queensland, Brisbane QLD 4072, Australia
    J Biol Chem 286:38814-24. 2011
    ....
  24. pmc Molecular determinants of ivermectin sensitivity at the glycine receptor chloride channel
    Timothy Lynagh
    Queensland Brain Institute, School of Biomedical Sciences, University of Queensland, Brisbane, Queensland, Australia
    J Biol Chem 286:43913-24. 2011
    ....
  25. ncbi request reprint Insights into the structural basis for zinc inhibition of the glycine receptor
    Simon T Nevin
    Department of Physiology and Pharmacology, School of Biomedical Sciences, University of Queensland, Brisbane, Queensland 4072, Australia
    J Biol Chem 278:28985-92. 2003
    ..The binding of zinc at the interface between adjacent alpha1 subunits could restrict intersubunit movements, providing a feasible mechanism for the inhibition of channel activation by zinc...
  26. pmc Magnitude of a conformational change in the glycine receptor beta1-beta2 loop is correlated with agonist efficacy
    Stephan A Pless
    Queensland Brain Institute and School of Biomedical Sciences, University of Queensland, Brisbane QLD 4072, Australia
    J Biol Chem 284:27370-6. 2009
    ..Our results support the conclusion that a closed-flip conformation change, with a magnitude proportional to the agonist affinity increase from closed to flip states, occurs in the microenvironment of Ala-52...
  27. ncbi request reprint Comparison of taurine- and glycine-induced conformational changes in the M2-M3 domain of the glycine receptor
    Nian Lin R Han
    School of Biomedical Sciences, University of Queensland, Brisbane, Queensland 4072, Australia
    J Biol Chem 279:19559-65. 2004
    ..We therefore conclude that the higher efficacy of glycine is due to an increased ability to stabilize a common activated configuration...
  28. doi request reprint GABAA receptors containing gamma1 subunits contribute to inhibitory transmission in the central amygdala
    Abolghasem Esmaeili
    The Queensland Brain Institute, The University of Queensland, Brisbane, QLD 4072, Australia
    J Neurophysiol 101:341-9. 2009
    ..These inputs arise from the intercalated cells masses, thought to be responsible for mediating extinction of conditioned fear, raising the possibility of new targets for the treatment of anxiety-related disorders...
  29. ncbi request reprint Molecular determinants of ginkgolide binding in the glycine receptor pore
    Rebecca Hawthorne
    School of Biomedical Sciences, University of Queensland, Brisbane, Queensland, Australia
    J Neurochem 98:395-407. 2006
    ..This identified an interaction between the variable R2 position of the ginkgolides and the 2' residues of both alpha1 and beta subunits. These findings provide strong evidence for ginkgolides binding at the 2' pore-lining position...
  30. doi request reprint Molecular determinants of beta-carboline inhibition of the glycine receptor
    Xuebin Chen
    Queensland Brain Institute and School of Biomedical Sciences, University of Queensland, Brisbane, Queensland 4072, Australia
    J Neurochem 110:1685-94. 2009
    ..The information may also be relevant to understanding the mechanism beta-carboline of binding to GABA type A receptors where they are potent pharmacological probes...
  31. doi request reprint Structure-activity analysis of ginkgolide binding in the glycine receptor pore
    Judith A Heads
    School of Biomedical Sciences, University of Queensland, Brisbane, QLD, Australia
    J Neurochem 105:1418-27. 2008
    ..This property may be exploited to create improved pharmacological probes for discriminating among anionic Cys-loop receptor isoforms with 6' structural variations...
  32. pmc β Subunit M2-M3 loop conformational changes are uncoupled from α1 β glycine receptor channel gating: implications for human hereditary hyperekplexia
    Qiang Shan
    Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia
    PLoS ONE 6:e28105. 2011
    ..Our study provides a possible explanation of why hereditary hyperekplexia-causing mutations that modify α1 β GlyR channel function are almost exclusively located in the α1 to the exclusion of the β subunit...
  33. ncbi request reprint Conformational variability of the glycine receptor M2 domain in response to activation by different agonists
    Stephan A Pless
    School of Biomedical Sciences, University of Queensland, Brisbane, Queensland 4072, Australia
    J Biol Chem 282:36057-67. 2007
    ..Thus, results from two separate labeled residues support the conclusion that the glycine receptor M2 domain responds with distinct conformational changes to activation by different agonists...
  34. doi request reprint Subunit-specific modulation of glycine receptors by cannabinoids and N-arachidonyl-glycine
    Zhe Yang
    Queensland Brain Institute and School of Biomedical Sciences, University of Queensland, Brisbane, QLD 4072, Australia
    Biochem Pharmacol 76:1014-23. 2008
    ..We conclude that cannabinoid agonists may be useful as pharmacological tools for selectively inhibiting alpha2 and alpha3 GlyRs. Our results also establish GlyRs as potential novel targets for endogenous and exogenous cannabinoids...
  35. pmc Ligand- and subunit-specific conformational changes in the ligand-binding domain and the TM2-TM3 linker of {alpha}1 {beta}2 {gamma}2 GABAA receptors
    Qian Wang
    Queensland Brain Institute and School of Biomedical Sciences, University of Queensland, Brisbane, Queensland 4072, Australia
    J Biol Chem 285:40373-86. 2010
    ..This result implies unexpected conformational mobility in this crucial part of the gating machinery. Together, this information provides new insights into the activation mechanisms of Cys-loop receptors...
  36. doi request reprint A comparison of glycine- and ivermectin-mediated conformational changes in the glycine receptor ligand-binding domain
    Qian Wang
    Queensland Brain Institute and School of Biomedical Sciences, University of Queensland, Brisbane, QLD 4072, Australia
    Int J Biochem Cell Biol 44:335-40. 2012
    ..This implies that conformational changes in these domains are important for activation. This result also provides a mechanism to explain how ivermectin potentiates glycine-induced channel activation...
  37. pmc An improved ivermectin-activated chloride channel receptor for inhibiting electrical activity in defined neuronal populations
    Timothy Lynagh
    Queensland Brain Institute and School of Biomedical Sciences, The University of Queensland, Brisbane QLD 4072, Australia
    J Biol Chem 285:14890-7. 2010
    ..As all known highly ivermectin-sensitive GluClRs contain an endogenous glycine residue at the corresponding location, this residue appears essential for exquisite ivermectin sensitivity...
  38. pmc New hyperekplexia mutations provide insight into glycine receptor assembly, trafficking, and activation mechanisms
    Anna Bode
    From the University of Queensland, Queensland Brain Institute and School of Biomedical Sciences, Queensland 4072, Australia
    J Biol Chem 288:33745-59. 2013
    ..To our knowledge, this is the first suggestion that subunits lacking TM4 domains might be incorporated into functional pentameric ligand-gated ion channel receptors. ..
  39. doi request reprint Ircinialactams: subunit-selective glycine receptor modulators from Australian sponges of the family Irciniidae
    Walter Balansa
    Institute for Molecular Bioscience, The University of Queensland, St Lucia, QLD 4072, Australia
    Bioorg Med Chem 18:2912-9. 2010
    ..Such GlyR modulators have potential application as pharmacological tools, and as leads for the development of GlyR targeting therapeutics to treat chronic inflammatory pain, epilepsy, spasticity and hyperekplexia...
  40. pmc GABAA receptor α and γ subunits shape synaptic currents via different mechanisms
    Christine Dixon
    From the Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia 4072
    J Biol Chem 289:5399-411. 2014
    ..Because this region determines post-synaptic localization, we hypothesize that GABAARs containing γ1 and γ2 use different mechanisms for synaptic clustering. ..
  41. ncbi request reprint Closed-state cross-linking of adjacent beta1 subunits in alpha1beta1 GABAa receptors via introduced 6' cysteines
    Zhe Yang
    School of Biomedical Sciences, University of Queensland, Brisbane QLD 4072, Australia
    J Biol Chem 282:16803-10. 2007
    ..Our results identify a novel feature of GABA(A)R gating that may be important for understanding its activation mechanism...
  42. pmc Fluorescence-based high-throughput functional profiling of ligand-gated ion channels at the level of single cells
    Sahil Talwar
    Queensland Brain Institute, The University of Queensland, Brisbane, Queensland, Australia
    PLoS ONE 8:e58479. 2013
    ....
  43. doi request reprint Optimizing the expression of recombinant alphabetagamma GABAA receptors in HEK293 cells for high-throughput screening
    Daniel Gilbert
    Queensland Brain Institute, University of Queensland, Brisbane, Australia, School of Biomedical Sciences, University of Queensland, Brisbane, Australia
    J Biomol Screen 14:86-91. 2009
    ..The authors conclude that this method of expressing and screening recombinant GABA(A)Rs provides an effective means of discovering novel GABA(A)R modulators for use as therapeutic lead compounds and pharmacological probes...
  44. doi request reprint A glycine residue essential for high ivermectin sensitivity in Cys-loop ion channel receptors
    Timothy Lynagh
    Queensland Brain Institute and School of Biomedical Sciences, The University of Queensland, Brisbane QLD, Australia
    Int J Parasitol 40:1477-81. 2010
    ..By providing a means of identifying ivermectin-sensitive receptors, this finding should help in characterising ivermectin-resistance mechanisms and identifying new anthelmintic targets...
  45. ncbi request reprint Comparative surface accessibility of a pore-lining threonine residue (T6') in the glycine and GABA(A) receptors
    Qiang Shan
    Department of Physiology and Pharmacology, School of Biomedical Sciences, University of Queensland, Brisbane, Queensland 4072, Australia
    J Biol Chem 277:44845-53. 2002
    ..The results also indicate that the GABA(A)R pore structure at the 6' level may vary between different expression systems...
  46. pmc The relative orientation of the TM3 and TM4 domains varies between α1 and α3 glycine receptors
    Lu Han
    Queensland Brain Institute, The University of Queensland, Brisbane QLD 4072, Australia
    ACS Chem Neurosci 4:248-54. 2013
    ..We conclude that the α1 and α3 GlyR TM4 domains are orientated differently relative to their TM3 domains. This may underlie their differential ability to influence glycine efficacy...
  47. ncbi request reprint Stoichiometry and subunit arrangement of α1β glycine receptors as determined by atomic force microscopy
    Zhe Yang
    Queensland Brain Institute, University of Queensland, Brisbane, QLD 4072, Australia
    Biochemistry 51:5229-31. 2012
    ..This permitted us to infer the number and relative locations of the respective subunits in functional pentamers. Our results indicate an invariant 2α1:3β stoichiometry with a β-α-β-α-β subunit arrangement...
  48. pmc Exon-skipping splice variants of excitatory amino acid transporter-2 (EAAT2) form heteromeric complexes with full-length EAAT2
    Florian M Gebhardt
    School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane 4072, Australia
    J Biol Chem 285:31313-24. 2010
    ..This could allow glutamate to accumulate extracellularly and promote excitotoxicity...
  49. doi request reprint Illuminating the structure and function of Cys-loop receptors
    Stephan A Pless
    School of Biomedical Sciences and Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia
    Clin Exp Pharmacol Physiol 35:1137-42. 2008
    ..They have shed new light on the conformational mobility of both the ligand-binding and the transmembrane domain of Cys-loop receptors...
  50. ncbi request reprint A yellow fluorescent protein-based assay for high-throughput screening of glycine and GABAA receptor chloride channels
    Wade Kruger
    School of Biomedical Sciences, University of Queensland, Brisbane, QLD 4072, Australia
    Neurosci Lett 380:340-5. 2005
    ..The robustness, sensitivity and low cost of this assay render it suited for high-throughput screening of transiently expressed anionic ligand-gated channels...
  51. doi request reprint Multiplexed labeling of viable cells for high-throughput analysis of glycine receptor function using flow cytometry
    Daniel F Gilbert
    Queensland Brain Institute, University of Queensland, Brisbane QLD 4072, Australia
    Cytometry A 75:440-9. 2009
    ..The present study demonstrates multiplexed labeling of live cells, to enable cell populations to be subject to further cell culture and experimentation, and compares the results with those obtained using live cell microscopy...
  52. pmc Mechanism of action of the insecticides, lindane and fipronil, on glycine receptor chloride channels
    Robiul Islam
    Queensland Brain Institute, The University of Queensland, Brisbane, Queensland, Australia
    Br J Pharmacol 165:2707-20. 2012
    ..We also investigated the mechanisms by which lindane and fipronil inhibit α1 glycine receptors...
  53. doi request reprint An outline of desensitization in pentameric ligand-gated ion channel receptors
    Angelo Keramidas
    Queensland Brain Institute, The University of Queensland, Brisbane, QLD, 4072, Australia
    Cell Mol Life Sci 70:1241-53. 2013
    ..However, major questions remain unresolved, including the structural basis of the desensitization-induced agonist affinity increase and the mechanism of pore closure during desensitization...
  54. ncbi request reprint Effects of polyunsaturated fatty acids on voltage-gated K+ and Na+ channels in rat olfactory receptor neurons
    Benchamaporn Seebungkert
    Department of Physiology and Pharmacology, School of Biomedical Sciences, University of Queensland, Brisbane, QLD, 4072, Australia
    Eur J Neurosci 16:2085-94. 2002
    ..These observations suggest that polyunsaturated fatty acids have the potential to strongly influence the coding of odorant information by olfactory receptor neurons...
  55. doi request reprint Chimera construction using multiple-template-based sequential PCRs
    Qiang Shan
    Queensland Brain Institute, University of Queensland, Brisbane, QLD, Australia
    J Neurosci Methods 193:86-9. 2010
    ..By introducing the "multiple-template" concept, this protocol only requires the use of two or three simple PCRs followed by general subcloning steps. Most importantly, the success rate is nearly 100%...
  56. ncbi request reprint Ligand-gated channels
    Peter H Barry
    School of Medical Sciences at the University of New South Wales, Sydney, NSW 2052, Australia
    IEEE Trans Nanobioscience 4:70-80. 2005
    ..The superfamily will mainly be exemplified by the excitatory nicotinic acetylcholine receptor (nAChR) and the inhibitory glycine receptor (GlyR) channels...