Research Topics
Genomes and Genes
| M H LittleSummaryAffiliation: University of Queensland Country: Australia Publications
Research Grants
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Detail Information
Publications
Tracing the life of the kidney tubule- re-establishing dogma and redirecting the optionsMelissa H Little
Australian Stem Cell Centre, The University of Queensland, Brisbane 4072, Australia
Cell Stem Cell 2:191-2. 2008..In this issue of Cell Stem Cell, Humphreys et al. (2008) use lineage tracing to genetically assess contribution of adult nontubular cells (potentially stem cells) to repair of damaged renal tubules...
Renal organogenesis: what can it tell us about renal repair and regeneration?Melissa H Little
Institute for Molecular Bioscience, The University of Queensland, St Lucia, Brisbane, Australia
Organogenesis 7:229-41. 2011....- Refining transcriptional programs in kidney development by integration of deep RNA-sequencing and array-based spatial profilingRathi D Thiagarajan
Institute for Molecular Bioscience, The University of Queensland, St, Lucia QLD 4072, Australia
BMC Genomics 12:441. 2011..These studies, however, fall short of capturing the transcriptional complexity arising from each locus due to the limited scope of microarray-based technology, which is largely based on "gene-centric" models... - A high-resolution anatomical ontology of the developing murine genitourinary tractMelissa H Little
Institute for Molecular Bioscience, University of Queensland, Brisbane 4072, Australia
Gene Expr Patterns 7:680-99. 2007..Visual examples of how terms appear in different specimen types are also provided... - Review article: Potential cellular therapies for renal disease: can we translate results from animal studies to the human condition?Melissa H Little
Institute for Molecular Bioscience, The University of Queensland, St Lucia, Queensland, Australia
Nephrology (Carlton) 14:544-53. 2009..In this review, we examine which animal models have been used to evaluate potential cellular therapies and how valid these are to human chronic kidney disease... 
Regrow or repair: potential regenerative therapies for the kidneyMelissa H Little
Institute for Molecular Bioscience, Queensland Bioscience Precinct, University of Queensland, St Lucia, Brisbane, QLD, 4072, Australia
J Am Soc Nephrol 17:2390-401. 2006....- Delivering on the promise of human stem-cell research. What are the real barriers?Melissa Little
Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia
EMBO Rep 7:1188-92. 2006 - Conserved modularity and potential for alternate splicing in mouse and human Slit genesMelissa Little
Institute for Molecular Bioscience and Centre for Functional and Applied Genomics, University of Queensland, St Lucia, Australia
Int J Dev Biol 46:385-91. 2002..This further implies the potential generation of multiple Slit protein isoforms varying in their number of repeat units. cDNA library screening and EST database searching verified that such alternate splicing does occur... - Dual trafficking of Slit3 to mitochondria and cell surface demonstrates novel localization for Slit proteinM H Little
Institute for Molecular Bioscience and Center for Functional and Applied Genomics, University of Queensland, St Lucia, 4072, Brisbane, Queensland, Australia
Am J Physiol Cell Physiol 281:C486-95. 2001..This is the first examination of Slit protein localization in nonneuronal cells, and this study implies that Slit3 has potentially unique functions not shared by other Slit proteins... - CRIM1, a novel gene encoding a cysteine-rich repeat protein, is developmentally regulated and implicated in vertebrate CNS development and organogenesisG Kolle
Centre for Molecular and Cellular Biology, The University of Queensland, Brisbane, Australia
Mech Dev 90:181-93. 2000..Our results suggest a role for CRIM1/Crim1 in CNS development possibly via growth factor binding... - Characterisation of Crim1 expression in the developing mouse urogenital tract reveals a sexually dimorphic gonadal expression patternK Georgas
Institute for Molecular Bioscience, incorporating the Centre for Molecular and Cellular Biology and the Special Research Centre for Functional and Applied Genomics, The University of Queensland, Brisbane, Australia
Dev Dyn 219:582-7. 2000..Crim1 also displays a striking male-specific expression pattern in the fetal gonads, its expression strongest in the Sertoli cells of the developing testis... - Distinct but overlapping expression patterns of two vertebrate slit homologs implies functional roles in CNS development and organogenesisG P Holmes
Centre for Molecular and Cellular Biology, University of Queensland, St Lucia, Brisbane, Australia
Mech Dev 79:57-72. 1998..Each gene shows additional but distinct sites of expression outside the CNS suggesting a variety of functions for these proteins... - Expression and functional analysis of Dkk1 during early gonadal developmentA N Combes
Division of Molecular Genetics and Development, Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD, Australia
Sex Dev 5:124-30. 2011..Nor did we find significant differences in expression of key markers of testis and ovarian development. We propose that DKK1 may play a protective role that is not unmasked by loss-of-function in the absence of other stressors... - Assignment of the human slit homologue SLIT2 to human chromosome band 4p15.2K Georgas
Centre for Molecular and Cellular Biology, University of Queensland, St. Lucia, Brisbane, Australia
Cytogenet Cell Genet 86:246-7. 1999 - Crim1 has an essential role in glycogen trophoblast cell and sinusoidal-trophoblast giant cell development in the placentaD J Pennisi
Institute for Molecular Bioscience, The University of Queensland, Brisbane 4072, Australia
Placenta 33:175-82. 2012..Our findings show that Crim1 is required for placental development, and is necessary for the proper differentiation of sinusoidal-trophoblast giant cells and glycogen trophoblast cells... - Stem cell options for kidney diseaseC Hopkins
Institute for Molecular Bioscience, University of Queensland, St Lucia, Australia
J Pathol 217:265-81. 2009..It also examines the remaining challenges and asks the question of whether there is one solution for all forms of renal disease... - Isolation of clonogenic, long-term self renewing embryonic renal stem cellsM Lusis
Institute for Molecular Bioscience, University of Queensland, St Lucia, 4072, Australia Australian Stem Cell Centre, Australia
Stem Cell Res 5:23-39. 2010..This embryonic renal stem cell population was not able to be isolated from postnatal kidney confirming that while the embryonic MM represents a mulitpotent stem cell population, this does not persist after birth... - Exogenous Slit2 does not affect ureteric branching or nephron formation during kidney developmentMichael Piper
Institute for Molecular Bioscience, and Department of Biochemistry, University of Queensland, Brisbane, Australia
Int J Dev Biol 46:545-50. 2002..In situ analysis of the Slit receptors, Robo1 and Robo2, the vasculogenic markers VEGFA and Flk-1, and the stromal cell marker BF2 displayed no difference in comparison to controls... - N-terminal Slit2 promotes survival and neurite extension in cultured peripheral neuronsMichael Piper
Institute for Molecular Bioscience, University of Queensland, Brisbane 4067, Australia
Neuroreport 13:2375-8. 2002..These findings suggest the Slit proteins may play an important role during development of the nervous system, mediating cellular survival in addition to the well documented role these proteins play in axonal and neuronal chemorepulsion... - CRIM1 regulates the rate of processing and delivery of bone morphogenetic proteins to the cell surfaceLorine Wilkinson
Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia
J Biol Chem 278:34181-8. 2003..The presence of CRIM1 reduced the effective BMP4 concentration of the media, thereby acting as a BMP4 antagonist. Hence, CRIM1 modulates BMP activity by affecting its processing and delivery to the cell surface... - Kidney development: two tales of tubulogenesisMelissa Little
Institute for Molecular Bioscience, The University of Queensland, St Lucia, Australia
Curr Top Dev Biol 90:193-229. 2010..Here we review both what is known and remains to be understood in kidney tubulogenesis... - Molecular anatomy of the kidney: what have we learned from gene expression and functional genomics?Bree Rumballe
Institute for Molecular Bioscience, The University of Queensland, St Lucia, 4072, Australia
Pediatr Nephrol 25:1005-16. 2010..Here we discuss how the advent of new genetic and genomics approaches has added to our understanding of kidney development and paediatric renal disease, as well as identifying areas in which we are still lacking knowledge... - Movement through Slits: cellular migration via the Slit familyMichael Piper
Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia
Bioessays 25:32-8. 2003..This review focuses on the non-neuronal activities of Slit proteins, highlighting a common role for the Slit family in cellular migration... - In ovo electroporation of Crim1 in the developing chick spinal cordGabriel Kolle
Institute for Molecular Bioscience, The University of Queensland, Brisbane, Australia
Dev Dyn 226:107-11. 2003..However, ectodomain CRIM1 overexpression leads to an approximate 50% decrease in populations of specific ventral neuronal populations, including ISL-1(+) motor neurons, CHX-10(+) V1, and EN-1(+) V2 interneurons... - Angioblast-mesenchyme induction of early kidney development is mediated by Wt1 and VegfaXiaobo Gao
Department of Medicine, Children s Hospital, and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
Development 132:5437-49. 2005.... - Kidney side population reveals multilineage potential and renal functional capacity but also cellular heterogeneityGrant A Challen
Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, QLD, 4072, Australia
J Am Soc Nephrol 17:1896-912. 2006..The heterogeneity of the renal SP highlights the need for further fractionation to distinguish the cellular subpopulations that are responsible for the observed multilineage capacity and transdifferentiative and humoral activities... - Spatial gene expression in the T-stage mouse metanephrosGeorgina Caruana
Department of Anatomy and Cell Biology, Monash University, Clayton, Vic, Australia
Gene Expr Patterns 6:807-25. 2006..This study has identified genes spatially expressed in regions of the developing kidney involved in branching morphogenesis, nephrogenesis and the development of the collecting duct system, calyces, renal pelvis and ureter... - Wnt-4 regulation by the Wilms' tumour suppressor gene, WT1Edmund U-H Sim
Institute for Molecular Bioscience, The University of Queensland, St Lucia, Brisbane, QLD 4072, Australia
Oncogene 21:2948-60. 2002..We propose that Wnt-4 regulation by WT1 occurs at a more distant promoter or enhancer site, or is indirect... - Involvement of Islet-2 in the Slit signaling for axonal branching and defasciculation of the sensory neurons in embryonic zebrafishSang Yeob Yeo
Laboratory for Developmental Gene Regulation, RIKEN Brain Science Institute, 2 1, Hirosawa, Wako, Saitama 351 0198, Japan
Mech Dev 121:315-24. 2004.... - PlexinA4 is necessary as a downstream target of Islet2 to mediate Slit signaling for promotion of sensory axon branchingToshio Miyashita
Laboratory for Developmental Gene Regulation, RIKEN Brain Science Institute, 2 1, Hirosawa, Wako, Saitama 351 0198, Japan
Development 131:3705-15. 2004.... - Differential gene expression in the developing mouse ureterEleanor K L Mitchell
Department of Anatomy and Cell Biology, School of Biomedical Sciences, Monash University, Clayton, Victoria 3800, Australia
Gene Expr Patterns 6:519-38. 2006..This study marks the first known report defining global gene expression of the developing mouse ureter and will provide insight into the molecular mechanisms underlying kidney and lower urinary tract malformations... - Identifying the molecular phenotype of renal progenitor cellsGrant A Challen
Institute for Molecular Bioscience, Queensland Bioscience Precinct, 306 Carmody Road, The University of Queensland, St Lucia, Brisbane, QLD, 4072, Australia
J Am Soc Nephrol 15:2344-57. 2004..These findings may assist in the isolation and characterization of potential renal stem cells for use in cellular therapies for kidney disease... - Establishment of metanephros transplantation in mice highlights contributions by both nephrectomy and pregnancy to developmental progressionShannon R Armstrong
Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Australia
Nephron Exp Nephrol 101:e155-64. 2005..This murine assay, when coupled with transgenically-tagged strains of mice, will allow the investigation of the relative contribution of donor and recipient cells to this process... - Renal structural and functional repair in a mouse model of reversal of ureteral obstructionAnita L Cochrane
Monash Immunology and Stem Cell Laboratories, Monash University, Victoria, Australia
J Am Soc Nephrol 16:3623-30. 2005..This study describes the regenerative potential of the kidney after the established interstitial matrix expansion and medullary ablation associated with UUO in the adult mouse... - Knockdown of zebrafish crim1 results in a bent tail phenotype with defects in somite and vascular developmentGenevieve Kinna
Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia
Mech Dev 123:277-87. 2006..Hence, the primary role of zebrafish crim1 is likely to be the regulation of somitic and vascular development... - Crim1KST264/KST264 mice implicate Crim1 in the regulation of vascular endothelial growth factor-A activity during glomerular vascular developmentLorine Wilkinson
Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia 4072
J Am Soc Nephrol 18:1697-708. 2007..This is the first in vivo demonstration of regulation of VEGF-A delivery and supports the hypothesis that Crim1 functions to regulate the release of growth factors from the cell of synthesis... - A side order of stem cells: the SP phenotypeGrant A Challen
Institute for Molecular Bioscience, Queensland Bioscience Precinct, 306 Carmody Road, The University of Queensland, St Lucia, Brisbane, QLD, 4072, Australia
Stem Cells 24:3-12. 2006.... - Crim1KST264/KST264 mice display a disruption of the Crim1 gene resulting in perinatal lethality with defects in multiple organ systemsDavid J Pennisi
Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland, Australia
Dev Dyn 236:502-11. 2007..The severe and complex phenotype observed in Crim1(KST264/KST264) mice highlights the importance of Crim1 in numerous aspects of organogenesis... - Neonatal calyceal dilation and renal fibrosis resulting from loss of Adamts-1 in mouse kidney is due to a developmental dysgenesisLaureane Mittaz
Monash Institute of Reproduction and Development, Monash University, Clayton 3168, Victoria, Australia
Nephrol Dial Transplant 20:419-23. 2005..Mice lacking this protein present with renal lesions comprising enlarged calyces, and reduced cortex and medulla layers. Our current findings are consistent with the defect occurring due to a developmental dysgenesis... - Definition and spatial annotation of the dynamic secretome during early kidney developmentGemma Martinez
Institute for Molecular Bioscience, The University of Queensland, Queensland, Australia
Dev Dyn 235:1709-19. 2006..A text-based anatomical ontology was used to spatially annotate the expression pattern of these genes in cultured metanephric explants... - Characterisation and trophic functions of murine embryonic macrophages based upon the use of a Csf1r-EGFP transgene reporterFiona Rae
Institute for Molecular Bioscience and ARC Special Research Centre for Functional and Applied Genomics, University of Queensland, Brisbane, Queensland 4072, Australia
Dev Biol 308:232-46. 2007..These findings suggest a trophic role of macrophages in embryonic kidney development, which may continue to play a similar role in postnatal repair...
Research Grants
- Towards renal regenerationMelissa Little; Fiscal Year: 2004..Human ES cell work will be performed using ES01, 02, 03, 04, 05 & 06 listed on the NIH ES cell line registry. ..
- Murine Atlas of Genitourinary DevelopmentMelissa Little; Fiscal Year: 2007....
- Murine Atlas of Genitourinary DevelopmentMelissa Little; Fiscal Year: 2007..abstract_text> ..