M H Little

Summary

Affiliation: University of Queensland
Country: Australia

Publications

  1. doi request reprint Tracing the life of the kidney tubule- re-establishing dogma and redirecting the options
    Melissa H Little
    Australian Stem Cell Centre, The University of Queensland, Brisbane 4072, Australia
    Cell Stem Cell 2:191-2. 2008
  2. pmc M2 macrophage polarisation is associated with alveolar formation during postnatal lung development
    Christina V Jones
    Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia
    Respir Res 14:41. 2013
  3. pmc Renal organogenesis: what can it tell us about renal repair and regeneration?
    Melissa H Little
    Institute for Molecular Bioscience, The University of Queensland, St Lucia, Brisbane, Australia
    Organogenesis 7:229-41. 2011
  4. pmc Refining transcriptional programs in kidney development by integration of deep RNA-sequencing and array-based spatial profiling
    Rathi D Thiagarajan
    Institute for Molecular Bioscience, The University of Queensland, St, Lucia QLD 4072, Australia
    BMC Genomics 12:441. 2011
  5. pmc A high-resolution anatomical ontology of the developing murine genitourinary tract
    Melissa H Little
    Institute for Molecular Bioscience, University of Queensland, Brisbane 4072, Australia
    Gene Expr Patterns 7:680-99. 2007
  6. pmc Delivering on the promise of human stem-cell research. What are the real barriers?
    Melissa Little
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia
    EMBO Rep 7:1188-92. 2006
  7. ncbi request reprint Regrow or repair: potential regenerative therapies for the kidney
    Melissa H Little
    Institute for Molecular Bioscience, Queensland Bioscience Precinct, University of Queensland, St Lucia, Brisbane, QLD, 4072, Australia
    J Am Soc Nephrol 17:2390-401. 2006
  8. ncbi request reprint Conserved modularity and potential for alternate splicing in mouse and human Slit genes
    Melissa Little
    Institute for Molecular Bioscience and Centre for Functional and Applied Genomics, University of Queensland, St Lucia, Australia
    Int J Dev Biol 46:385-91. 2002
  9. ncbi request reprint Dual trafficking of Slit3 to mitochondria and cell surface demonstrates novel localization for Slit protein
    M H Little
    Institute for Molecular Bioscience and Center for Functional and Applied Genomics, University of Queensland, St Lucia, 4072, Brisbane, Queensland, Australia
    Am J Physiol Cell Physiol 281:C486-95. 2001
  10. doi request reprint Review article: Potential cellular therapies for renal disease: can we translate results from animal studies to the human condition?
    Melissa H Little
    Institute for Molecular Bioscience, The University of Queensland, St Lucia, Queensland, Australia
    Nephrology (Carlton) 14:544-53. 2009

Research Grants

  1. Towards renal regeneration
    Melissa Little; Fiscal Year: 2004
  2. Murine Atlas of Genitourinary Development
    Melissa Little; Fiscal Year: 2007
  3. Murine Atlas of Genitourinary Development
    Melissa Little; Fiscal Year: 2007

Collaborators

Detail Information

Publications44

  1. doi request reprint Tracing the life of the kidney tubule- re-establishing dogma and redirecting the options
    Melissa H Little
    Australian Stem Cell Centre, The University of Queensland, Brisbane 4072, Australia
    Cell Stem Cell 2:191-2. 2008
    ..In this issue of Cell Stem Cell, Humphreys et al. (2008) use lineage tracing to genetically assess contribution of adult nontubular cells (potentially stem cells) to repair of damaged renal tubules...
  2. pmc M2 macrophage polarisation is associated with alveolar formation during postnatal lung development
    Christina V Jones
    Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia
    Respir Res 14:41. 2013
    ..However, the role of macrophages in lung development and the potential implications of macrophage modulation in the promotion of lung maturation have not yet been ascertained...
  3. pmc Renal organogenesis: what can it tell us about renal repair and regeneration?
    Melissa H Little
    Institute for Molecular Bioscience, The University of Queensland, St Lucia, Brisbane, Australia
    Organogenesis 7:229-41. 2011
    ....
  4. pmc Refining transcriptional programs in kidney development by integration of deep RNA-sequencing and array-based spatial profiling
    Rathi D Thiagarajan
    Institute for Molecular Bioscience, The University of Queensland, St, Lucia QLD 4072, Australia
    BMC Genomics 12:441. 2011
    ..These studies, however, fall short of capturing the transcriptional complexity arising from each locus due to the limited scope of microarray-based technology, which is largely based on "gene-centric" models...
  5. pmc A high-resolution anatomical ontology of the developing murine genitourinary tract
    Melissa H Little
    Institute for Molecular Bioscience, University of Queensland, Brisbane 4072, Australia
    Gene Expr Patterns 7:680-99. 2007
    ..Visual examples of how terms appear in different specimen types are also provided...
  6. pmc Delivering on the promise of human stem-cell research. What are the real barriers?
    Melissa Little
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia
    EMBO Rep 7:1188-92. 2006
  7. ncbi request reprint Regrow or repair: potential regenerative therapies for the kidney
    Melissa H Little
    Institute for Molecular Bioscience, Queensland Bioscience Precinct, University of Queensland, St Lucia, Brisbane, QLD, 4072, Australia
    J Am Soc Nephrol 17:2390-401. 2006
    ....
  8. ncbi request reprint Conserved modularity and potential for alternate splicing in mouse and human Slit genes
    Melissa Little
    Institute for Molecular Bioscience and Centre for Functional and Applied Genomics, University of Queensland, St Lucia, Australia
    Int J Dev Biol 46:385-91. 2002
    ..This further implies the potential generation of multiple Slit protein isoforms varying in their number of repeat units. cDNA library screening and EST database searching verified that such alternate splicing does occur...
  9. ncbi request reprint Dual trafficking of Slit3 to mitochondria and cell surface demonstrates novel localization for Slit protein
    M H Little
    Institute for Molecular Bioscience and Center for Functional and Applied Genomics, University of Queensland, St Lucia, 4072, Brisbane, Queensland, Australia
    Am J Physiol Cell Physiol 281:C486-95. 2001
    ..This is the first examination of Slit protein localization in nonneuronal cells, and this study implies that Slit3 has potentially unique functions not shared by other Slit proteins...
  10. doi request reprint Review article: Potential cellular therapies for renal disease: can we translate results from animal studies to the human condition?
    Melissa H Little
    Institute for Molecular Bioscience, The University of Queensland, St Lucia, Queensland, Australia
    Nephrology (Carlton) 14:544-53. 2009
    ..In this review, we examine which animal models have been used to evaluate potential cellular therapies and how valid these are to human chronic kidney disease...
  11. ncbi request reprint CRIM1, a novel gene encoding a cysteine-rich repeat protein, is developmentally regulated and implicated in vertebrate CNS development and organogenesis
    G Kolle
    Centre for Molecular and Cellular Biology, The University of Queensland, Brisbane, Australia
    Mech Dev 90:181-93. 2000
    ..Our results suggest a role for CRIM1/Crim1 in CNS development possibly via growth factor binding...
  12. ncbi request reprint Characterisation of Crim1 expression in the developing mouse urogenital tract reveals a sexually dimorphic gonadal expression pattern
    K Georgas
    Institute for Molecular Bioscience, incorporating the Centre for Molecular and Cellular Biology and the Special Research Centre for Functional and Applied Genomics, The University of Queensland, Brisbane, Australia
    Dev Dyn 219:582-7. 2000
    ..Crim1 also displays a striking male-specific expression pattern in the fetal gonads, its expression strongest in the Sertoli cells of the developing testis...
  13. ncbi request reprint Distinct but overlapping expression patterns of two vertebrate slit homologs implies functional roles in CNS development and organogenesis
    G P Holmes
    Centre for Molecular and Cellular Biology, University of Queensland, St Lucia, Brisbane, Australia
    Mech Dev 79:57-72. 1998
    ..Each gene shows additional but distinct sites of expression outside the CNS suggesting a variety of functions for these proteins...
  14. pmc Expression and functional analysis of Dkk1 during early gonadal development
    A N Combes
    Division of Molecular Genetics and Development, Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD, Australia
    Sex Dev 5:124-30. 2011
    ..Nor did we find significant differences in expression of key markers of testis and ovarian development. We propose that DKK1 may play a protective role that is not unmasked by loss-of-function in the absence of other stressors...
  15. ncbi request reprint Assignment of the human slit homologue SLIT2 to human chromosome band 4p15.2
    K Georgas
    Centre for Molecular and Cellular Biology, University of Queensland, St Lucia, Brisbane, Australia
    Cytogenet Cell Genet 86:246-7. 1999
  16. doi request reprint Directing human embryonic stem cell differentiation towards a renal lineage generates a self-organizing kidney
    M Takasato
    Institute for Molecular Bioscience, The University of Queensland, St Lucia 4072, Queensland, Australia
    Nat Cell Biol 16:118-26. 2014
    ..Such hESC-derived components show broad renal potential ex vivo, illustrating the potential for pluripotent-stem-cell-based renal regeneration. ..
  17. doi request reprint Crim1 has an essential role in glycogen trophoblast cell and sinusoidal-trophoblast giant cell development in the placenta
    D J Pennisi
    Institute for Molecular Bioscience, The University of Queensland, Brisbane 4072, Australia
    Placenta 33:175-82. 2012
    ..Our findings show that Crim1 is required for placental development, and is necessary for the proper differentiation of sinusoidal-trophoblast giant cells and glycogen trophoblast cells...
  18. doi request reprint Stem cell options for kidney disease
    C Hopkins
    Institute for Molecular Bioscience, University of Queensland, St Lucia, Australia
    J Pathol 217:265-81. 2009
    ..It also examines the remaining challenges and asks the question of whether there is one solution for all forms of renal disease...
  19. doi request reprint Isolation of clonogenic, long-term self renewing embryonic renal stem cells
    M Lusis
    Institute for Molecular Bioscience, University of Queensland, St Lucia, 4072, Australia Australian Stem Cell Centre, Australia
    Stem Cell Res 5:23-39. 2010
    ..This embryonic renal stem cell population was not able to be isolated from postnatal kidney confirming that while the embryonic MM represents a mulitpotent stem cell population, this does not persist after birth...
  20. ncbi request reprint N-terminal Slit2 promotes survival and neurite extension in cultured peripheral neurons
    Michael Piper
    Institute for Molecular Bioscience, University of Queensland, Brisbane 4067, Australia
    Neuroreport 13:2375-8. 2002
    ..These findings suggest the Slit proteins may play an important role during development of the nervous system, mediating cellular survival in addition to the well documented role these proteins play in axonal and neuronal chemorepulsion...
  21. ncbi request reprint Exogenous Slit2 does not affect ureteric branching or nephron formation during kidney development
    Michael Piper
    Institute for Molecular Bioscience, and Department of Biochemistry, University of Queensland, Brisbane, Australia
    Int J Dev Biol 46:545-50. 2002
    ..In situ analysis of the Slit receptors, Robo1 and Robo2, the vasculogenic markers VEGFA and Flk-1, and the stromal cell marker BF2 displayed no difference in comparison to controls...
  22. doi request reprint Modelling cell turnover in a complex tissue during development
    J Lefevre
    Institute for Molecular Bioscience, The University of Queensland, St Lucia, Brisbane, QLD 4072, Australia
    J Theor Biol 338:66-79. 2013
    ....
  23. ncbi request reprint CRIM1 regulates the rate of processing and delivery of bone morphogenetic proteins to the cell surface
    Lorine Wilkinson
    Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia
    J Biol Chem 278:34181-8. 2003
    ..The presence of CRIM1 reduced the effective BMP4 concentration of the media, thereby acting as a BMP4 antagonist. Hence, CRIM1 modulates BMP activity by affecting its processing and delivery to the cell surface...
  24. doi request reprint Kidney development: two tales of tubulogenesis
    Melissa Little
    Institute for Molecular Bioscience, The University of Queensland, St Lucia, Australia
    Curr Top Dev Biol 90:193-229. 2010
    ..Here we review both what is known and remains to be understood in kidney tubulogenesis...
  25. pmc Molecular anatomy of the kidney: what have we learned from gene expression and functional genomics?
    Bree Rumballe
    Institute for Molecular Bioscience, The University of Queensland, St Lucia, 4072, Australia
    Pediatr Nephrol 25:1005-16. 2010
    ..Here we discuss how the advent of new genetic and genomics approaches has added to our understanding of kidney development and paediatric renal disease, as well as identifying areas in which we are still lacking knowledge...
  26. ncbi request reprint In ovo electroporation of Crim1 in the developing chick spinal cord
    Gabriel Kolle
    Institute for Molecular Bioscience, The University of Queensland, Brisbane, Australia
    Dev Dyn 226:107-11. 2003
    ..However, ectodomain CRIM1 overexpression leads to an approximate 50% decrease in populations of specific ventral neuronal populations, including ISL-1(+) motor neurons, CHX-10(+) V1, and EN-1(+) V2 interneurons...
  27. ncbi request reprint Movement through Slits: cellular migration via the Slit family
    Michael Piper
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia
    Bioessays 25:32-8. 2003
    ..This review focuses on the non-neuronal activities of Slit proteins, highlighting a common role for the Slit family in cellular migration...
  28. ncbi request reprint Angioblast-mesenchyme induction of early kidney development is mediated by Wt1 and Vegfa
    Xiaobo Gao
    Department of Medicine, Children s Hospital, and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
    Development 132:5437-49. 2005
    ....
  29. ncbi request reprint Kidney side population reveals multilineage potential and renal functional capacity but also cellular heterogeneity
    Grant A Challen
    Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, QLD, 4072, Australia
    J Am Soc Nephrol 17:1896-912. 2006
    ..The heterogeneity of the renal SP highlights the need for further fractionation to distinguish the cellular subpopulations that are responsible for the observed multilineage capacity and transdifferentiative and humoral activities...
  30. ncbi request reprint Spatial gene expression in the T-stage mouse metanephros
    Georgina Caruana
    Department of Anatomy and Cell Biology, Monash University, Clayton, Vic, Australia
    Gene Expr Patterns 6:807-25. 2006
    ..This study has identified genes spatially expressed in regions of the developing kidney involved in branching morphogenesis, nephrogenesis and the development of the collecting duct system, calyces, renal pelvis and ureter...
  31. ncbi request reprint Crim1KST264/KST264 mice implicate Crim1 in the regulation of vascular endothelial growth factor-A activity during glomerular vascular development
    Lorine Wilkinson
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia 4072
    J Am Soc Nephrol 18:1697-708. 2007
    ..This is the first in vivo demonstration of regulation of VEGF-A delivery and supports the hypothesis that Crim1 functions to regulate the release of growth factors from the cell of synthesis...
  32. ncbi request reprint Wnt-4 regulation by the Wilms' tumour suppressor gene, WT1
    Edmund U H Sim
    Institute for Molecular Bioscience, The University of Queensland, St Lucia, Brisbane, QLD 4072, Australia
    Oncogene 21:2948-60. 2002
    ..We propose that Wnt-4 regulation by WT1 occurs at a more distant promoter or enhancer site, or is indirect...
  33. ncbi request reprint Involvement of Islet-2 in the Slit signaling for axonal branching and defasciculation of the sensory neurons in embryonic zebrafish
    Sang Yeob Yeo
    Laboratory for Developmental Gene Regulation, RIKEN Brain Science Institute, 2 1, Hirosawa, Wako, Saitama 351 0198, Japan
    Mech Dev 121:315-24. 2004
    ....
  34. ncbi request reprint PlexinA4 is necessary as a downstream target of Islet2 to mediate Slit signaling for promotion of sensory axon branching
    Toshio Miyashita
    Laboratory for Developmental Gene Regulation, RIKEN Brain Science Institute, 2 1, Hirosawa, Wako, Saitama 351 0198, Japan
    Development 131:3705-15. 2004
    ....
  35. ncbi request reprint Identifying the molecular phenotype of renal progenitor cells
    Grant A Challen
    Institute for Molecular Bioscience, Queensland Bioscience Precinct, 306 Carmody Road, The University of Queensland, St Lucia, Brisbane, QLD, 4072, Australia
    J Am Soc Nephrol 15:2344-57. 2004
    ..These findings may assist in the isolation and characterization of potential renal stem cells for use in cellular therapies for kidney disease...
  36. ncbi request reprint Definition and spatial annotation of the dynamic secretome during early kidney development
    Gemma Martinez
    Institute for Molecular Bioscience, The University of Queensland, Queensland, Australia
    Dev Dyn 235:1709-19. 2006
    ..A text-based anatomical ontology was used to spatially annotate the expression pattern of these genes in cultured metanephric explants...
  37. ncbi request reprint Neonatal calyceal dilation and renal fibrosis resulting from loss of Adamts-1 in mouse kidney is due to a developmental dysgenesis
    Laureane Mittaz
    Monash Institute of Reproduction and Development, Monash University, Clayton 3168, Victoria, Australia
    Nephrol Dial Transplant 20:419-23. 2005
    ..Mice lacking this protein present with renal lesions comprising enlarged calyces, and reduced cortex and medulla layers. Our current findings are consistent with the defect occurring due to a developmental dysgenesis...
  38. ncbi request reprint Establishment of metanephros transplantation in mice highlights contributions by both nephrectomy and pregnancy to developmental progression
    Shannon R Armstrong
    Institute for Molecular Bioscience, The University of Queensland, St Lucia, Australia
    Nephron Exp Nephrol 101:e155-64. 2005
    ..The aim of this study was to adapt this approach for use in mice and examine the parameters affecting successful onward development in this species...
  39. ncbi request reprint Crim1KST264/KST264 mice display a disruption of the Crim1 gene resulting in perinatal lethality with defects in multiple organ systems
    David J Pennisi
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland, Australia
    Dev Dyn 236:502-11. 2007
    ..The severe and complex phenotype observed in Crim1(KST264/KST264) mice highlights the importance of Crim1 in numerous aspects of organogenesis...
  40. ncbi request reprint Differential gene expression in the developing mouse ureter
    Eleanor K L Mitchell
    Department of Anatomy and Cell Biology, School of Biomedical Sciences, Monash University, Clayton, Victoria 3800, Australia
    Gene Expr Patterns 6:519-38. 2006
    ..This study marks the first known report defining global gene expression of the developing mouse ureter and will provide insight into the molecular mechanisms underlying kidney and lower urinary tract malformations...
  41. ncbi request reprint Knockdown of zebrafish crim1 results in a bent tail phenotype with defects in somite and vascular development
    Genevieve Kinna
    Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia
    Mech Dev 123:277-87. 2006
    ..Hence, the primary role of zebrafish crim1 is likely to be the regulation of somitic and vascular development...
  42. ncbi request reprint Renal structural and functional repair in a mouse model of reversal of ureteral obstruction
    Anita L Cochrane
    Monash Immunology and Stem Cell Laboratories, Monash University, Victoria, Australia
    J Am Soc Nephrol 16:3623-30. 2005
    ..This study describes the regenerative potential of the kidney after the established interstitial matrix expansion and medullary ablation associated with UUO in the adult mouse...
  43. ncbi request reprint Characterisation and trophic functions of murine embryonic macrophages based upon the use of a Csf1r-EGFP transgene reporter
    Fiona Rae
    Institute for Molecular Bioscience and ARC Special Research Centre for Functional and Applied Genomics, University of Queensland, Brisbane, Queensland 4072, Australia
    Dev Biol 308:232-46. 2007
    ..These findings suggest a trophic role of macrophages in embryonic kidney development, which may continue to play a similar role in postnatal repair...
  44. ncbi request reprint A side order of stem cells: the SP phenotype
    Grant A Challen
    Institute for Molecular Bioscience, Queensland Bioscience Precinct, 306 Carmody Road, The University of Queensland, St Lucia, Brisbane, QLD, 4072, Australia
    Stem Cells 24:3-12. 2006
    ....

Research Grants5

  1. Towards renal regeneration
    Melissa Little; Fiscal Year: 2004
    ..Human ES cell work will be performed using ES01, 02, 03, 04, 05 & 06 listed on the NIH ES cell line registry. ..
  2. Murine Atlas of Genitourinary Development
    Melissa Little; Fiscal Year: 2007
    ....
  3. Murine Atlas of Genitourinary Development
    Melissa Little; Fiscal Year: 2007
    ..abstract_text> ..