Pawel Listwan

Summary

Affiliation: University of Queensland
Country: Australia

Publications

  1. ncbi request reprint Modification of recombinatorial cloning for small affinity tag fusion protein construct generation
    Pawel Listwan
    School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland 4072, Australia
    Anal Biochem 346:327-9. 2005
  2. ncbi request reprint Overview of the pipeline for structural and functional characterization of macrophage proteins at the university of queensland
    Weining Meng
    School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Australia
    Methods Mol Biol 426:577-87. 2008
  3. ncbi request reprint A general target selection method for crystallographic proteomics
    Gautier Robin
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia
    Methods Mol Biol 426:27-35. 2008
  4. ncbi request reprint Focusing in on structural genomics: the University of Queensland structural biology pipeline
    Munish Puri
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland, Australia
    Biomol Eng 23:281-9. 2006
  5. ncbi request reprint Incorporating a TEV cleavage site reduces the solubility of nine recombinant mouse proteins
    Mareike Kurz
    Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia
    Protein Expr Purif 50:68-73. 2006
  6. ncbi request reprint An automatable screen for the rapid identification of proteins amenable to refolding
    Nathan P Cowieson
    Institute for Molecular Bioscience and ARC Special Research Centre for Functional and Applied Genomics, University of Queensland, Brisbane, Queensland, Australia
    Proteomics 6:1750-7. 2006
  7. ncbi request reprint An inflammatory role for the mammalian carboxypeptidase inhibitor latexin: relationship to cystatins and the tumor suppressor TIG1
    Anna Aagaard
    Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia
    Structure 13:309-17. 2005
  8. pmc Crystal structures of fusion proteins with large-affinity tags
    Douglas R Smyth
    Department of Biochemistry and Molecular Biology, Institute for Molecular Bioscience, and Special Research Centre for Functional and Applied Genomics, University of Queensland, St Lucia, Queensland 4072, Australia
    Protein Sci 12:1313-22. 2003
  9. ncbi request reprint Substrate specificity of protein kinases and computational prediction of substrates
    Bostjan Kobe
    School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Australia
    Biochim Biophys Acta 1754:200-9. 2005
  10. ncbi request reprint Pilot studies on the parallel production of soluble mouse proteins in a bacterial expression system
    Nathan P Cowieson
    Institute for Molecular Bioscience, Australia
    J Struct Funct Genomics 6:13-20. 2005

Collaborators

Detail Information

Publications10

  1. ncbi request reprint Modification of recombinatorial cloning for small affinity tag fusion protein construct generation
    Pawel Listwan
    School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland 4072, Australia
    Anal Biochem 346:327-9. 2005
  2. ncbi request reprint Overview of the pipeline for structural and functional characterization of macrophage proteins at the university of queensland
    Weining Meng
    School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Australia
    Methods Mol Biol 426:577-87. 2008
    ..Jointly, the data sheds light on the molecular and cellular functions of macrophage proteins...
  3. ncbi request reprint A general target selection method for crystallographic proteomics
    Gautier Robin
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia
    Methods Mol Biol 426:27-35. 2008
    ..This novel procedure was designed to maximize selection efficiency, and its prevailing criteria categories make it suitable for a broad range of structural proteomics projects...
  4. ncbi request reprint Focusing in on structural genomics: the University of Queensland structural biology pipeline
    Munish Puri
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland, Australia
    Biomol Eng 23:281-9. 2006
    ..The structures from this pipeline will provide insights into the function of previously uncharacterized macrophage proteins and could lead to the validation of new drug targets for chronic obstructive pulmonary disease and arthritis...
  5. ncbi request reprint Incorporating a TEV cleavage site reduces the solubility of nine recombinant mouse proteins
    Mareike Kurz
    Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia
    Protein Expr Purif 50:68-73. 2006
    ..Our work suggests that one needs to be very careful when making modifications to expression vectors and combining different affinity and fusion tags and cleavage sites...
  6. ncbi request reprint An automatable screen for the rapid identification of proteins amenable to refolding
    Nathan P Cowieson
    Institute for Molecular Bioscience and ARC Special Research Centre for Functional and Applied Genomics, University of Queensland, Brisbane, Queensland, Australia
    Proteomics 6:1750-7. 2006
    ..The efficacy of the screen is demonstrated on small-scale expression samples for 15 proteins. Refolding is then validated by large-scale expressions using SEC and circular dichroism...
  7. ncbi request reprint An inflammatory role for the mammalian carboxypeptidase inhibitor latexin: relationship to cystatins and the tumor suppressor TIG1
    Anna Aagaard
    Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia
    Structure 13:309-17. 2005
    ..The structure of the tumor suppressor protein TIG1 was modeled, revealing its putative membrane binding surface...
  8. pmc Crystal structures of fusion proteins with large-affinity tags
    Douglas R Smyth
    Department of Biochemistry and Molecular Biology, Institute for Molecular Bioscience, and Special Research Centre for Functional and Applied Genomics, University of Queensland, St Lucia, Queensland 4072, Australia
    Protein Sci 12:1313-22. 2003
    ..g., membrane proteins). Structural genomics initiatives may also benefit from this approach as a way to crystallize problematic proteins of significant interest...
  9. ncbi request reprint Substrate specificity of protein kinases and computational prediction of substrates
    Bostjan Kobe
    School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Australia
    Biochim Biophys Acta 1754:200-9. 2005
    ..We discuss the structural basis of substrate specificity of protein kinases and review the experimental and computational methods used to obtain specificity information...
  10. ncbi request reprint Pilot studies on the parallel production of soluble mouse proteins in a bacterial expression system
    Nathan P Cowieson
    Institute for Molecular Bioscience, Australia
    J Struct Funct Genomics 6:13-20. 2005
    ..This success rate compares favourably with other protein screening projects, particularly for eukaryotic proteins, and could be further improved by modifications at the cloning step...