Nicole L La Gruta

Summary

Affiliation: University of Melbourne
Country: Australia

Publications

  1. doi request reprint Multiplexed combinatorial tetramer staining in a mouse model of virus infection
    Tania Cukalac
    Department of Microbiology and Immunology, University of Melbourne, Parkville 3010, Melbourne, Australia
    J Immunol Methods 360:157-61. 2010
  2. pmc Interrogating the relationship between naïve and immune antiviral T cell repertoires
    Nicole L La Gruta
    Department of Microbiology and Immunology, University of Melbourne, Parkville, VIC 3010, Australia
    Curr Opin Virol 3:447-51. 2013
  3. ncbi request reprint Hierarchies in cytokine expression profiles for acute and resolving influenza virus-specific CD8+ T cell responses: correlation of cytokine profile and TCR avidity
    Nicole L La Gruta
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Vic, Australia
    J Immunol 172:5553-60. 2004
  4. pmc Primary CTL response magnitude in mice is determined by the extent of naive T cell recruitment and subsequent clonal expansion
    Nicole L La Gruta
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia
    J Clin Invest 120:1885-94. 2010
  5. ncbi request reprint A correlation between function and selected measures of T cell avidity in influenza virus-specific CD8+ T cell responses
    Nicole L La Gruta
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Australia
    Eur J Immunol 36:2951-9. 2006
  6. ncbi request reprint A question of self-preservation: immunopathology in influenza virus infection
    Nicole L La Gruta
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia
    Immunol Cell Biol 85:85-92. 2007
  7. pmc A virus-specific CD8+ T cell immunodominance hierarchy determined by antigen dose and precursor frequencies
    Nicole L La Gruta
    Department of Microbiology and Immunology, University of Melbourne, Parkville 3010, Australia
    Proc Natl Acad Sci U S A 103:994-9. 2006
  8. pmc Protective efficacy of cross-reactive CD8+ T cells recognising mutant viral epitopes depends on peptide-MHC-I structural interactions and T cell activation threshold
    Sophie A Valkenburg
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Australia
    PLoS Pathog 6:e1001039. 2010
  9. pmc Epitope-specific TCRbeta repertoire diversity imparts no functional advantage on the CD8+ T cell response to cognate viral peptides
    Nicole L La Gruta
    Department of Microbiology and Immunology, University of Melbourne, Parkville 3010, Victoria, Australia
    Proc Natl Acad Sci U S A 105:2034-9. 2008
  10. pmc Structural basis for enabling T-cell receptor diversity within biased virus-specific CD8+ T-cell responses
    E Bridie Day
    Department of Microbiology and Immunology, Bio21, Department of Biochemistry, University of Melbourne, Parkville, VIC 3010, Australia
    Proc Natl Acad Sci U S A 108:9536-41. 2011

Collaborators

Detail Information

Publications37

  1. doi request reprint Multiplexed combinatorial tetramer staining in a mouse model of virus infection
    Tania Cukalac
    Department of Microbiology and Immunology, University of Melbourne, Parkville 3010, Melbourne, Australia
    J Immunol Methods 360:157-61. 2010
    ....
  2. pmc Interrogating the relationship between naïve and immune antiviral T cell repertoires
    Nicole L La Gruta
    Department of Microbiology and Immunology, University of Melbourne, Parkville, VIC 3010, Australia
    Curr Opin Virol 3:447-51. 2013
    ..Finally, we describe how TCR sequencing has delineated the relationship between naïve and immune T cell repertoires. ..
  3. ncbi request reprint Hierarchies in cytokine expression profiles for acute and resolving influenza virus-specific CD8+ T cell responses: correlation of cytokine profile and TCR avidity
    Nicole L La Gruta
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Vic, Australia
    J Immunol 172:5553-60. 2004
    ....
  4. pmc Primary CTL response magnitude in mice is determined by the extent of naive T cell recruitment and subsequent clonal expansion
    Nicole L La Gruta
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia
    J Clin Invest 120:1885-94. 2010
    ..These findings suggest possibilities for enhancing protective immune memory by maximizing both the size and diversity of typically subdominant T cell responses through rational vaccine design...
  5. ncbi request reprint A correlation between function and selected measures of T cell avidity in influenza virus-specific CD8+ T cell responses
    Nicole L La Gruta
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Australia
    Eur J Immunol 36:2951-9. 2006
    ..Furthermore, this study shows that different indicators of avidity do not necessarily provide similar information and should be used in combination to obtain an overall picture of the characteristics of TCR binding...
  6. ncbi request reprint A question of self-preservation: immunopathology in influenza virus infection
    Nicole L La Gruta
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia
    Immunol Cell Biol 85:85-92. 2007
    ....
  7. pmc A virus-specific CD8+ T cell immunodominance hierarchy determined by antigen dose and precursor frequencies
    Nicole L La Gruta
    Department of Microbiology and Immunology, University of Melbourne, Parkville 3010, Australia
    Proc Natl Acad Sci U S A 103:994-9. 2006
    ..Thus, this immunodominance hierarchy is a direct function of antigen dose and T cell numbers...
  8. pmc Protective efficacy of cross-reactive CD8+ T cells recognising mutant viral epitopes depends on peptide-MHC-I structural interactions and T cell activation threshold
    Sophie A Valkenburg
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Australia
    PLoS Pathog 6:e1001039. 2010
    ..Our study does not support vaccine strategies that include immunization against commonly selected cross-reactive variants with mutations at partially-solvent exposed residues that have characteristics comparable to NPN3A...
  9. pmc Epitope-specific TCRbeta repertoire diversity imparts no functional advantage on the CD8+ T cell response to cognate viral peptides
    Nicole L La Gruta
    Department of Microbiology and Immunology, University of Melbourne, Parkville 3010, Victoria, Australia
    Proc Natl Acad Sci U S A 105:2034-9. 2008
    ..Thus, whereas a diverse repertoire may be important for recognition of epitope variants, its effect on the response to cognate pMHC recognition appears minimal...
  10. pmc Structural basis for enabling T-cell receptor diversity within biased virus-specific CD8+ T-cell responses
    E Bridie Day
    Department of Microbiology and Immunology, Bio21, Department of Biochemistry, University of Melbourne, Parkville, VIC 3010, Australia
    Proc Natl Acad Sci U S A 108:9536-41. 2011
    ..Overall, these data provide an explanation for the combination of TCRV region bias and diversity within selected repertoires, even as they maintain exquisite pMHCI specificity...
  11. ncbi request reprint The influenza virus-specific CTL immunodominance hierarchy in mice is determined by the relative frequency of high-avidity T cells
    Tania Cukalac
    Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia
    J Immunol 192:4061-8. 2014
    ..Our study strongly indicates that the quality, and not simply the quantity, of antiviral CTLs dictate response magnitude. ..
  12. ncbi request reprint Cell cycle-related acquisition of cytotoxic mediators defines the progressive differentiation to effector status for virus-specific CD8+ T cells
    Misty R Jenkins
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia
    J Immunol 181:3818-22. 2008
    ..Thus, while effector gene expression can be acquired early, maturation of cytotoxic capacity requires extended differentiation...
  13. pmc Terminal deoxynucleotidyltransferase is required for the establishment of private virus-specific CD8+ TCR repertoires and facilitates optimal CTL responses
    Katherine Kedzierska
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Australia
    J Immunol 181:2556-62. 2008
    ..The key finding is thus that the role of TdT in ensuring enhanced diversity and the selection of private TCR repertoires promotes optimal CD8(+) T cell immunity, both within individuals and across the species as a whole...
  14. pmc Tracking phenotypically and functionally distinct T cell subsets via T cell repertoire diversity
    Katherine Kedzierska
    Department of Microbiology and Immunology, University of Melbourne, Parkville 3010, Melbourne, Australia
    Mol Immunol 45:607-18. 2008
    ....
  15. ncbi request reprint Reproducible selection of high avidity CD8+ T-cell clones following secondary acute virus infection
    Tania Cukalac
    Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, VIC 3010, Australia
    Proc Natl Acad Sci U S A 111:1485-90. 2014
    ..Despite this shift in clonotype representation indicative of differential selection, there was no evidence of overall repertoire narrowing, suggesting a strategy to optimize CTL responses while safeguarding TCR diversity. ..
  16. doi request reprint Unlike CD4+ T-cell help, CD28 costimulation is necessary for effective primary CD8+ T-cell influenza-specific immunity
    Shirley G K Seah
    Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Australia
    Eur J Immunol 42:1744-54. 2012
    ..We suggest that direct CD28 costimulation of CD8(+) T cells is more critical in their priming during primary influenza infection than previously appreciated...
  17. pmc Functional implications of T cell receptor diversity
    Stephen J Turner
    Department of Microbiology and Immunology, The University of Melbourne, Victoria, Australia
    Curr Opin Immunol 21:286-90. 2009
    ..Here, we review recent advances demonstrating a link between T cell repertoire diversity and immunity to infection, and consider the potential mechanisms at play...
  18. ncbi request reprint Lack of prominent peptide-major histocompatibility complex features limits repertoire diversity in virus-specific CD8+ T cell populations
    Stephen J Turner
    Department of Microbiology and Immunology, The University of Melbourne, Parkville, Victoria 3010, Australia
    Nat Immunol 6:382-9. 2005
    ..Thus, the lack of prominent features in an antigenic complex of peptide and major histocompatibility complex class I is associated with a diminished spectrum of TCR usage...
  19. ncbi request reprint Characterization of CD8+ T cell repertoire diversity and persistence in the influenza A virus model of localized, transient infection
    Stephen J Turner
    Department of Microbiology and Immunology, University of Melbourne, Parkville, VIC 3010, Australia
    Semin Immunol 16:179-84. 2004
    ..The approach offers novel insights into the factors determining the selection of immune repertoires, and their functional consequences for CD8(+) T cell-mediated immunity...
  20. ncbi request reprint Establishment and recall of CD8+ T-cell memory in a model of localized transient infection
    Katherine Kedzierska
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Melbourne, Australia
    Immunol Rev 211:133-45. 2006
    ....
  21. pmc Contribution of T cell receptor affinity to overall avidity for virus-specific CD8+ T cell responses
    Katherine Kedzierska
    Department of Microbiology and Immunology, University of Melbourne, Parkville 3010, Melbourne, Australia
    Proc Natl Acad Sci U S A 102:11432-7. 2005
    ..Thus, whereas TCR sequence (or affinity) appears to contribute substantially to the avidity profile of diverse virus-specific CD8+ populations, other mechanisms may be prominent where the TCR spectrum is more limited...
  22. doi request reprint Influenza epitope-specific CD8+ T cell avidity, but not cytokine polyfunctionality, can be determined by TCRβ clonotype
    Jessica M Moffat
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia
    J Immunol 185:6850-6. 2010
    ..These data suggest that factors other than TCRβ sequence influence cytokine profiles and demonstrate no link between differential avidity and polyfunctionality...
  23. ncbi request reprint IL-18, but not IL-12, is required for optimal cytokine production by influenza virus-specific CD8+ T cells
    Alice E Denton
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Australia
    Eur J Immunol 37:368-75. 2007
    ..Together, our results suggest that IL-18, but not IL-12, induces optimal, antigen-specific production of key cytokines by CD8+ T cells for the efficient clearance of influenza virus from the lungs of infected mice...
  24. doi request reprint Killer T cells in influenza
    John Stambas
    Department of Microbiology and Immunology, University of Melbourne, Royal Parade, Parkville, Victoria 3010, Australia
    Pharmacol Ther 120:186-96. 2008
    ..Understanding the complexities of CD8+ T cell mediated immunity and memory has the potential for improving vaccine design, particularly to combat pandemics caused by newly emerging influenza viruses...
  25. pmc Dendritic cell preactivation impairs MHC class II presentation of vaccines and endogenous viral antigens
    Louise J Young
    The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia
    Proc Natl Acad Sci U S A 104:17753-8. 2007
    ..These observations have important implications for the design of prophylactic and therapeutic DC vaccines and contribute to the understanding of the mechanisms causing immunosuppression during systemic blood infections...
  26. pmc Effect of MHC class I diversification on influenza epitope-specific CD8+ T cell precursor frequency and subsequent effector function
    E Bridie Day
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3010, Australia
    J Immunol 186:6319-28. 2011
    ..Such findings illustrate the difficulties of predicting and defining the effects of MHC class I diversification on epitope-specific responses...
  27. pmc A trans-Golgi network golgin is required for the regulated secretion of TNF in activated macrophages in vivo
    Zi Zhao Lieu
    Department of Biochemistry and Molecular Biology and Bio21 Molecular Science and Biotechnology Institute and Department of Microbiology and Immunology, University of Melbourne, Victoria 3010, Australia
    Proc Natl Acad Sci U S A 105:3351-6. 2008
    ..Also, we have demonstrated a previously undescribed approach to control cytokine secretion by the specific silencing of trafficking machinery...
  28. doi request reprint Precursor frequency and competition dictate the HLA-A2-restricted CD8+ T cell responses to influenza A infection and vaccination in HLA-A2.1 transgenic mice
    Amabel C L Tan
    Department of Microbiology and Immunology, University of Melbourne, Parkville 3010, Victoria, Australia
    J Immunol 187:1895-902. 2011
    ....
  29. doi request reprint The linear range for accurately quantifying antigen-specific T-cell frequencies by tetramer staining during natural immune responses
    Shirley G K Seah
    The Walter and Eliza Hall Institute of Medical Research, Parkville, Australia
    Eur J Immunol 41:1499-500. 2011
  30. ncbi request reprint Thymic expression of a gastritogenic epitope results in positive selection of self-reactive pathogenic T cells
    Karen L Laurie
    Department of Biochemistry and Molecular Biology, University of Melbourne, Parkville, Australia Division of Immunobiology, University of Bonn, Bonn, Germany
    J Immunol 172:5994-6002. 2004
    ..These findings also suggest that the precise level of tissue-specific Ags in the thymus may be an important consideration in protection against autoimmune disease and that perturbation of the levels of self-Ags may be detrimental...
  31. ncbi request reprint Immunopathogenesis, loss of T cell tolerance and genetics of autoimmune gastritis
    Ian R van Driel
    The Russell Grimwade School of Biochemistry and Molecular Biology, The University of Melbourne, Parkville, Victoria 3010, Australia
    Autoimmun Rev 1:290-7. 2002
    ..The highlights of these recent advances are the subject of this review...
  32. ncbi request reprint Reliable generation and use of MHC class II:gamma2aFc multimers for the identification of antigen-specific CD4(+) T cells
    Paula Y Arnold
    Department of Immunology, St Jude Children s Research Hospital, 332 N Lauderdale, Memphis, TN 38105, USA
    J Immunol Methods 271:137-51. 2002
    ..Furthermore, we have successfully used these reagents to stain T cells generated following viral infection. Thus, MHC class II:gamma2aFc multimers are robust and reliable reagents for the analysis of MHC class II-restricted T cells...
  33. pmc Differential tumor necrosis factor receptor 2-mediated editing of virus-specific CD8+ effector T cells
    Stephen J Turner
    Department of Immunology, St Jude Children s Research Hospital, 332 North Lauderdale Street, Memphis, TN 38105, USA
    Proc Natl Acad Sci U S A 101:3545-50. 2004
    ..Therefore, the massive difference in magnitude for the secondary, although not the primary, response to these D(b)NP(366) and D(b)PA(224) epitopes cannot be considered to reflect differential TNFR2-mediated T cell editing...
  34. pmc Effector CD8+ T cells recovered from an influenza pneumonia differentiate to a state of focused gene expression
    Dana R Marshall
    Department of Immunology, Hartwell Center for Bioinformatics, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Proc Natl Acad Sci U S A 102:6074-9. 2005
    ....
  35. ncbi request reprint Differential regulation of gastric tumor growth by cytokines that signal exclusively through the coreceptor gp130
    Meegan Howlett
    Department of Medicine, University of Melbourne at Western Hospital, Footscray, Australia
    Gastroenterology 129:1005-18. 2005
    ..This model recapitulates many characteristics of intestinal-type gastric cancer in humans...
  36. ncbi request reprint The majority of immunogenic epitopes generate CD4+ T cells that are dependent on MHC class II-bound peptide-flanking residues
    Paula Y Arnold
    Department of Immunology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    J Immunol 169:739-49. 2002
    ..This key feature, which is not shared by MHC class I-restricted responses, may underlie the broad functional diversity displayed by MHC class II-restricted T cells...
  37. ncbi request reprint Architectural changes in the TCR:CD3 complex induced by MHC:peptide ligation
    Nicole L La Gruta
    Department of Immunology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    J Immunol 172:3662-9. 2004
    ..These data suggest a mechanism by which ligated TCR may be differentiated from unligated TCR and selectively down-modulated...