S J Kent

Summary

Affiliation: University of Melbourne
Country: Australia

Publications

  1. pmc Complexity of the inoculum determines the rate of reversion of SIV Gag CD8 T cell mutant virus and outcome of infection
    Liyen Loh
    Department of Microbiology and Immunology, University of Melbourne, Melbourne, Victoria, Australia
    PLoS Pathog 5:e1000378. 2009
  2. pmc Control of viremia and prevention of AIDS following immunotherapy of SIV-infected macaques with peptide-pulsed blood
    Robert De Rose
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia
    PLoS Pathog 4:e1000055. 2008
  3. pmc Influence of cytokines on HIV-specific antibody-dependent cellular cytotoxicity activation profile of natural killer cells
    Leia Wren
    Department of Microbiology and Immunology, University of Melbourne, Victoria, Australia
    PLoS ONE 7:e38580. 2012
  4. pmc Comparison of influenza and SIV specific CD8 T cell responses in macaques
    Sinthujan Jegaskanda
    Department of Microbiology and Immunology, University of Melbourne, Melbourne, Victoria, Australia
    PLoS ONE 7:e32431. 2012
  5. pmc An "escape clock" for estimating the turnover of SIV DNA in resting CD4⁺ T cells
    Jeanette Reece
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia
    PLoS Pathog 8:e1002615. 2012
  6. pmc Antibody-Dependent Cellular Cytotoxicity and NK Cell-Driven Immune Escape in HIV Infection: Implications for HIV Vaccine Development
    Gamze Isitman
    Department of Microbiology and Immunology, University of Melbourne, Melbourne, VIC 3010, Australia
    Adv Virol 2012:637208. 2012
  7. pmc Measuring Turnover of SIV DNA in Resting CD4+ T Cells Using Pyrosequencing: Implications for the Timing of HIV Eradication Therapies
    Jeanette C Reece
    Department of Microbiology and Immunology, University of Melbourne, Victoria, Australia
    PLoS ONE 9:e93330. 2014
  8. ncbi HIV-specific antibody immunity mediated through NK cells and monocytes
    Marit Kramski
    Department of Microbiology and Immunology, University of Melbourne, Parkville, 3010, Australia
    Curr HIV Res 11:388-406. 2013
  9. doi The search for an HIV cure: tackling latent infection
    Stephen J Kent
    Department of Microbiology and Immunology, University of Melbourne, Vic, Australia
    Lancet Infect Dis 13:614-21. 2013
  10. pmc HIV infection abrogates the functional advantage of natural killer cells educated through KIR3DL1/HLA-Bw4 interactions to mediate anti-HIV antibody-dependent cellular cytotoxicity
    Matthew S Parsons
    Research Institute of the McGill University Health Centre, Montreal, Quebec, Canada
    J Virol 86:4488-95. 2012

Collaborators

Detail Information

Publications84

  1. pmc Complexity of the inoculum determines the rate of reversion of SIV Gag CD8 T cell mutant virus and outcome of infection
    Liyen Loh
    Department of Microbiology and Immunology, University of Melbourne, Melbourne, Victoria, Australia
    PLoS Pathog 5:e1000378. 2009
    ..The delay in reversion of fitness-reducing CD8 T cell escape mutations in some scenarios suggests opportunities to reduce the pathogenicity of HIV during very early infection...
  2. pmc Control of viremia and prevention of AIDS following immunotherapy of SIV-infected macaques with peptide-pulsed blood
    Robert De Rose
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia
    PLoS Pathog 4:e1000055. 2008
    ..Our results suggest Gag alone is an effective antigen for T-cell immunotherapy. Fresh blood cells pulsed with overlapping Gag peptides is proceeding into trials in HIV-infected humans...
  3. pmc Influence of cytokines on HIV-specific antibody-dependent cellular cytotoxicity activation profile of natural killer cells
    Leia Wren
    Department of Microbiology and Immunology, University of Melbourne, Victoria, Australia
    PLoS ONE 7:e38580. 2012
    ..Manipulating the immunological environment to enhance the potency of NK cell-mediated HIV-specific ADCC effector functions could be a promising immunotherapy or vaccine strategy...
  4. pmc Comparison of influenza and SIV specific CD8 T cell responses in macaques
    Sinthujan Jegaskanda
    Department of Microbiology and Immunology, University of Melbourne, Melbourne, Victoria, Australia
    PLoS ONE 7:e32431. 2012
    ..Our results suggest that strategies to maintain a more functional CD8(+) T-cell response, profile may assist in controlling HIV disease...
  5. pmc An "escape clock" for estimating the turnover of SIV DNA in resting CD4⁺ T cells
    Jeanette Reece
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia
    PLoS Pathog 8:e1002615. 2012
    ..Our results suggest that, in order to reduce the formation of a stable population of SIV DNA, it will be important either to intervene very early or intervene during active replication...
  6. pmc Antibody-Dependent Cellular Cytotoxicity and NK Cell-Driven Immune Escape in HIV Infection: Implications for HIV Vaccine Development
    Gamze Isitman
    Department of Microbiology and Immunology, University of Melbourne, Melbourne, VIC 3010, Australia
    Adv Virol 2012:637208. 2012
    ..These newer finding suggest NK cells and ADCC responses apply significant pressure to the virus. There is an opportunity to harness these immune responses in the design of more effective HIV vaccines...
  7. pmc Measuring Turnover of SIV DNA in Resting CD4+ T Cells Using Pyrosequencing: Implications for the Timing of HIV Eradication Therapies
    Jeanette C Reece
    Department of Microbiology and Immunology, University of Melbourne, Victoria, Australia
    PLoS ONE 9:e93330. 2014
    ..0084). Our results suggest viral DNA within resting CD4 T cells is more labile and may be more susceptible to reactivation/eradication treatments when there are higher levels of virus replication and during early/acute infection. ..
  8. ncbi HIV-specific antibody immunity mediated through NK cells and monocytes
    Marit Kramski
    Department of Microbiology and Immunology, University of Melbourne, Parkville, 3010, Australia
    Curr HIV Res 11:388-406. 2013
    ..In summary, this review highlights recent advances in HIV-specific antibody immunity mediated through NK cells and monocytes. ..
  9. doi The search for an HIV cure: tackling latent infection
    Stephen J Kent
    Department of Microbiology and Immunology, University of Melbourne, Vic, Australia
    Lancet Infect Dis 13:614-21. 2013
    ..Clinical studies that use a longer duration of drug dosing, alternative agents, combination approaches, gene therapy, and immune-modulation approaches are all underway. ..
  10. pmc HIV infection abrogates the functional advantage of natural killer cells educated through KIR3DL1/HLA-Bw4 interactions to mediate anti-HIV antibody-dependent cellular cytotoxicity
    Matthew S Parsons
    Research Institute of the McGill University Health Centre, Montreal, Quebec, Canada
    J Virol 86:4488-95. 2012
    ..The abrogation of the functional advantage of educated NK cells may enhance HIV disease progression. Strategies to enhance the potency of NK cell-mediated ADCC may improve HIV therapies and vaccines...
  11. pmc The testis and epididymis are productively infected by SIV and SHIV in juvenile macaques during the post-acute stage of infection
    Miranda Shehu-Xhilaga
    Infectious Diseases Unit, Alfred Hospital, Prahran, Australia
    Retrovirology 4:7. 2007
    ..Little is known about the progression and pathogenesis of HIV-1 infection within the male genital tract (MGT), particularly during the early stages of infection...
  12. ncbi Knowledge and commitment for action: the 14th International AIDS Conference, Barcelona, July 2002
    Stephen J Kent
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Vic
    Med J Aust 177:587-9. 2002
  13. pmc Vaccination with attenuated simian immunodeficiency virus by DNA inoculation
    S J Kent
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3010, Australia
    J Virol 75:11930-4. 2001
    ..Injection of SIV(mac239) DNA containing identical deletions in both the 5' LTR and 3' nef/LTR resulted in attenuated SIV infections and substantial protection against subsequent mucosal SIV(mac251) challenge...
  14. ncbi Determining the immune mechanisms of protection from AIDS: correlates of immunity and the development of syngeneic macaques
    S J Kent
    Dept of Microbiology and Immunology, University of Melbourne, Parkville, Vic, Australia
    Immunol Rev 183:94-108. 2001
    ..The review also focuses on the development of syngeneic macaques by twinning and cloning technologies...
  15. ncbi Drug evaluation: DNA/MVA prime-boost HIV vaccine
    Stephen Kent
    University of Melbourne, Department of Microbiology and Immunology, Melbourne, Victoria 3010, Australia
    Curr Opin Investig Drugs 8:159-67. 2007
    ..HIVA) then boosting with a MVA virus expressing HIVA (MVA.HIVA). Phase II clinical trials of the vaccine are underway in Kenya and the UK...
  16. ncbi Evidence of recombination between 3' and 5' LTRs in macaques inoculated with SIV DNA
    Stephen J Kent
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Vic, 3010, Australia
    AIDS Res Hum Retroviruses 18:227-30. 2002
    ..This sequence analysis is the first demonstration of homologous recombination in vivo following administration of a single SIV strain...
  17. ncbi Reversion of immune escape HIV variants upon transmission: insights into effective viral immunity
    Stephen J Kent
    Department of Microbiology and Immunology, University of Melbourne, Victoria 3010, Australia
    Trends Microbiol 13:243-6. 2005
    ..A virus crippled by immune escape mutations would result in reduced viral load and delayed disease. Such knowledge could be used to rationally design more effective vaccines...
  18. ncbi Vaccines for HIV in the developing world
    S J Kent
    Department of Microbiology and Immunology, University of Melbourne, Victoria, Australia
    Intern Med J 32:136-7. 2002
  19. doi Evaluation of recombinant Kunjin replicon SIV vaccines for protective efficacy in macaques
    Stephen J Kent
    Department of Microbiology and Immunology, University of Melbourne, 3010, Australia
    Virology 374:528-34. 2008
    ..Refinements of this vector system and optimization of the immunization doses, routes, and schedules are required prior to clinical trials...
  20. ncbi Mucosally-administered human-simian immunodeficiency virus DNA and fowlpoxvirus-based recombinant vaccines reduce acute phase viral replication in macaques following vaginal challenge with CCR5-tropic SHIVSF162P3
    Stephen J Kent
    Department of Microbiology and Immunology, University of Melbourne, VIC 3010, Australia
    Vaccine 23:5009-21. 2005
    ..Our data suggest strategies for effective priming of partial immunity to mucosal HIV-1 exposure utilizing systemic prime and mucosal boost vaccination strategies...
  21. ncbi Attenuated and wild-type HIV-1 infections and long terminal repeat-mediated gene expression from plasmids delivered by gene gun to human skin ex vivo and macaques in vivo
    S J Kent
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia
    Virology 287:71-8. 2001
    ..Thus, although attenuated lentiviral vaccines might be deliverable as proviral DNA constructs in primate subjects, significant improvements are needed to enhance the efficiency of this method...
  22. pmc Enhanced T-cell immunogenicity and protective efficacy of a human immunodeficiency virus type 1 vaccine regimen consisting of consecutive priming with DNA and boosting with recombinant fowlpox virus
    S J Kent
    AIDS Pathogenesis Research Unit, Macfarlane Burnet Centre for Medical Research, Fairfield 3078, Victoria, Australia
    J Virol 72:10180-8. 1998
    ..These studies suggest a safe strategy for the enhanced generation of T-cell-mediated protective immunity to HIV-1...
  23. pmc Enhanced cellular immunity in macaques following a novel peptide immunotherapy
    S Chea
    Department of Microbiology and Immunology, University of Melbourne, Victoria 3010, Australia
    J Virol 79:3748-57. 2005
    ..De novo hepatitis C virus-specific CD4+- and CD8+-T-cell responses were generated in macaques by the same method. This simple technique holds promise for the immunotherapy of HIV and other chronic viral infections...
  24. pmc Efficacy of DNA and fowlpox virus priming/boosting vaccines for simian/human immunodeficiency virus
    C J Dale
    Department of Microbiology and Immunology, University of Melbourne, Victoria 3010, Australia
    J Virol 78:13819-28. 2004
    ..These studies suggest that priming of immunity with DNA and fowlpox virus vaccines could delay AIDS in humans...
  25. ncbi Subtype AE HIV-1 DNA and recombinant Fowlpoxvirus vaccines encoding five shared HIV-1 genes: safety and T cell immunogenicity in macaques
    Robert De Rose
    Department of Microbiology and Immunology, University of Melbourne, Royal Parade, VIC 3010, Australia
    Vaccine 23:1949-56. 2005
    ..In conclusion, these subtype AE HIV-1 DNA and rFPV vaccines were safe, induced broad T-cell immunity in macaques, and are suitable for progression into clinical trials...
  26. ncbi Evaluation in macaques of HIV-1 DNA vaccines containing primate CpG motifs and fowlpoxvirus vaccines co-expressing IFNgamma or IL-12
    C Jane Dale
    Department of Microbiology and Immunology, University of Melbourne, Royal Parade, VIC 3010, Australia
    Vaccine 23:188-97. 2004
    ..Non-sterilising protection from a non-pathogenic HIV-1 challenge was observed. This study provides evidence of a safe, optimised, strategy for the generation of T-cell mediated immunity to HIV-1...
  27. ncbi Chimeric human papilloma virus-simian/human immunodeficiency virus virus-like-particle vaccines: immunogenicity and protective efficacy in macaques
    C Jane Dale
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3010, Australia
    Virology 301:176-87. 2002
    ....
  28. ncbi Nucleic acid vaccines: tasks and tactics
    B S McKenzie
    The Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Parkville, Australia
    Immunol Res 24:225-44. 2001
    ..In this review, we have taken the liberty to discuss these enhancement methods, whenever possible, in the context of the underlying mechanisms that might argue for or against these strategies...
  29. pmc Evaluation of recombinant influenza virus-simian immunodeficiency virus vaccines in macaques
    Amy Sexton
    Department of Microbiology and Immunology, University of Melbourne, Victoria 3010, Australia
    J Virol 83:7619-28. 2009
    ..Although our results suggest that influenza virus-HIV vaccines hold promise for the induction of mucosal immunity to HIV, broader antigen cover will be needed to limit cytotoxic T-lymphocyte escape...
  30. pmc Comparative efficacy of subtype AE simian-human immunodeficiency virus priming and boosting vaccines in pigtail macaques
    Robert De Rose
    Department of Microbiology and Immunology, University of Melbourne, Victoria 3010, Australia
    J Virol 81:292-300. 2007
    ..These studies suggest priming of T-cell immunity to prevent AIDS in humans is possible, but differences in the immunogenicity of various subtype vaccines and broad cross-subtype protection are substantial hurdles...
  31. ncbi Prime-boost strategies in DNA vaccines
    C Jane Dale
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia
    Methods Mol Med 127:171-97. 2006
    ..Methods for the construction of the vaccines, the use of animal models, and the detection of immune responses are described...
  32. ncbi Genetically identical primate modelling systems for HIV vaccines
    S J Kent
    AIDS Pathogenesis Research Unit, Macfarlane Burnet Centre for Medical Research, Fairfield, Victoria, Australia
    Reprod Fertil Dev 10:651-7. 1998
    ..Despite the technical difficulties to be overcome, the potential gains in human health from the development of genetically identical non-human primates are worthy of careful consideration...
  33. ncbi Decreased neurotropism of nef long terminal repeat (nef/LTR)-deleted simian immunodeficiency virus
    Katherine A Thompson
    Department of Microbiology and Immunology, University of Melbourne, Melbourne, Australia
    J Neurovirol 9:442-51. 2003
    ....
  34. ncbi Phenotypic and kinetic analysis of effective simian-human immunodeficiency virus-specific T cell responses in DNA--and fowlpox virus-vaccinated macaques
    Ivan Stratov
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3010, Australia
    Virology 337:222-34. 2005
    ..These results shed light on some of the requirements for T cells in the control of pathogenic lentiviral infections...
  35. pmc In-vivo stimulation of macaque natural killer T cells with α-galactosylceramide
    C S Fernandez
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Vic, Australia
    Clin Exp Immunol 173:480-92. 2013
    ..Accordingly, although macaque peripheral NKT cells are modulated by α-GalCer in vivo, at least for the dosing regimens tested in this study, this does not appear to have a significant impact on anti-viral immunity in macaque models. ..
  36. doi Induction of HIV-1 subtype B and AE-specific neutralizing antibodies in mice and macaques with DNA prime and recombinant gp140 protein boost regimens
    Rob J Center
    Department of Microbiology and Immunology, University of Melbourne, Parkville, 3010 Vic, Australia
    Vaccine 27:6605-12. 2009
    ..Although the vaccines tested provide a platform for inducing robust humoral immunity, further refinements to broaden coverage against divergent strains and induce mucosal immunity are needed...
  37. pmc Induction of T-cell immunity to antiretroviral drug-resistant human immunodeficiency virus type 1
    Ivan Stratov
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3010, Australia
    J Virol 79:7728-37. 2005
    ..This study suggests feasible strategies to further evaluate the potential of limiting antiretroviral drug resistance through induction of T-cell immunity...
  38. pmc Inactivated simian immunodeficiency virus-pulsed autologous fresh blood cells as an immunotherapy strategy
    Rosemarie D Mason
    Department of Microbiology and Immunology, University of Melbourne, Melbourne 3010, Australia
    J Virol 83:1501-10. 2009
    ..Future studies of inactivated virus-pulsed blood cell immunotherapy during early infection of patients receiving antiretroviral therapy are warranted...
  39. ncbi Short communication: characteristics of effective immune control of simian/human immunodeficiency virus in pigtail macaques
    Ivan Stratov
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3010, Australia
    AIDS Res Hum Retroviruses 22:27-32. 2006
    ..These observations, although in a small subset of animals, suggest the hypothesis that combinations of primed T cell immunity and neutralizing antibodies can maintain control of chronic primate lentiviral infections...
  40. ncbi Comparative evaluation of simian, simian-human, and human immunodeficiency virus infections in the pigtail macaque (Macaca nemestrina) model
    C Jane Batten
    Department of Microbiology and Immunology, University of Melbourne, Victoria 3010, Australia
    AIDS Res Hum Retroviruses 22:580-8. 2006
    ..Our results provide a series of virologic models with which to evaluate potential AIDS vaccines in pigtail macaques...
  41. pmc Vaccine-induced T cells control reversion of AIDS virus immune escape mutants
    Caroline S Fernandez
    Department of Microbiology and Immunology, University of Melbourne, Melbourne, Victoria, and Department of Haematology, Prince of Wales Hospital, Kensington, NSW, Australia
    J Virol 81:4137-44. 2007
    ..These findings have important implications for the further development of T-cell-based HIV vaccines where exposure to escape mutant viruses is common...
  42. pmc Balancing reversion of cytotoxic T-lymphocyte and neutralizing antibody escape mutations within human immunodeficiency virus type 1 Env upon transmission
    Viv Peut
    Department of Microbiology and Immunology, University of Melbourne, Melbourne, Australia
    J Virol 83:8986-92. 2009
    ..At one site studied, both CTL and NLGS mutations were needed to confer NAb escape. We conclude that CTL and NAb escape within Env can be tightly linked, suggesting opportunities to induce effective multicomponent anti-Env immunity...
  43. ncbi Dose-response relationship of DNA and recombinant fowlpox virus prime-boost HIV vaccines: implications for future trials
    Robert De Rose
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia
    Hum Vaccin 2:134-6. 2006
    ..When adjusted for body surface area, the human dose studied was equivalent to a poorly immunogenic lower dose in monkeys. These data provide a rationale for guiding dosing in future trials of HIV vaccine technologies...
  44. ncbi Granulocyte contamination dramatically inhibits spot formation in AIDS virus-specific ELISpot assays: analysis and strategies to ameliorate
    Robert De Rose
    Department of Microbiology and Immunology, University of Melbourne, Royal Parade, Parkville, Victoria 3010, Australia
    J Immunol Methods 297:177-86. 2005
    ..Improving the standard ELISpot assay by using an F(ab)(2) capture antibody should make it more reliable for use in critical vaccine development studies...
  45. pmc Utility of human immunodeficiency virus type 1 envelope as a T-cell immunogen
    Viv Peut
    Department of Microbiology and Immunology, University of Melbourne, Parkville 3010, Australia
    J Virol 81:13125-34. 2007
    ..These results suggest that Env may have limited utility as a CD8 T-cell immunogen...
  46. ncbi Fitness constraints on immune escape from HIV: Implications of envelope as a target for both HIV-specific T cells and antibody
    Viv Peut
    Department of Microbiology and Immunology, University of Melbourne, 3010, Australia
    Curr HIV Res 4:191-7. 2006
    ..This could ultimately be exploited in HIV vaccine design...
  47. doi Substantial envelope-specific CD8 T-cell immunity fails to control SIV disease
    Viv Peut
    Department of Microbiology and Immunology, University of Melbourne, Parkville Victoria, Australia
    Virology 384:21-7. 2009
    ..We conclude Env-specific CTL may not be the most effective response to induce by vaccination...
  48. ncbi Role of monocytes in mediating HIV-specific antibody-dependent cellular cytotoxicity
    M Kramski
    Department of Microbiology and Immunology, University of Melbourne, Australia
    J Immunol Methods 384:51-61. 2012
    ..We conclude that the RFADCC assay primarily reflects Ab-mediated monocyte function. Further studies on the immunological importance of HIV-specific monocyte-mediated ADCC are warranted...
  49. pmc Peripheral NKT cells in simian immunodeficiency virus-infected macaques
    Caroline S Fernandez
    Department of Microbiology and Immunology, University of Melbourne, Australia
    J Virol 83:1617-24. 2009
    ..Further studies of the implications of the loss of NKT cell subsets in the pathogenesis of HIV disease are needed...
  50. doi Protection afforded by live attenuated SIV is associated with rapid killing kinetics of CTLs
    Erik Rollman
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Vic, Australia
    J Med Primatol 37:24-32. 2008
    ..We hypothesized that long-lived antigen exposure from live attenuated SIV may result in CD8+ cytotoxic T lymphocytes persistently capable of rapidly cytolytic potential...
  51. doi Differential patterns of immune escape at Tat-specific cytotoxic T cell epitopes in pigtail macaques
    Rosemarie D Mason
    Department of Microbiology and Immunology, University of Melbourne 3010, Australia
    Virology 388:315-23. 2009
    ..Our results suggest that some regulatory or accessory CTL epitopes may be useful targets for vaccination against HIV...
  52. ncbi Killing kinetics of simian immunodeficiency virus-specific CD8+ T cells: implications for HIV vaccine strategies
    Erik Rollman
    Department of Microbiology and Immunology, University of Melbourne, Victoria, Australia
    J Immunol 179:4571-9. 2007
    ..The delayed cytolytic phenotype exhibited by standard vector-based vaccine-induced CTLs may limit the ability of T cell-based HIV vaccines to rapidly control acute infection following a pathogenic lentiviral exposure...
  53. ncbi Vaccines and vaccine strategies against HIV
    Ivan Stratov
    Department of Immunology and Microbiology, University of Melbourne, Victoria 3010, Australia
    Curr Drug Targets 5:71-88. 2004
    ..Although an HIV vaccine is still a long way off, there is reason to be optimistic that a vaccine to prevent AIDS will eventually be developed...
  54. doi Low pre-infection levels and loss of central memory CD4+ T cells may predict rapid progression in SIV-infected pigtail macaques
    Rosemarie D Mason
    Department of Microbiology and Immunology, University of Melbourne, 3010 Australia
    Virology 381:11-5. 2008
    ..If confirmed in larger studies, our results suggest stratifying macaques for baseline central memory CD4+ T cells would be useful in defining both the pathogenesis of SIV disease and SIV vaccine efficacy...
  55. ncbi Genetic influences on HIV infection: implications for vaccine development
    Miranda Z Smith
    Department of Microbiology and Immunology, University of Melbourne, Parkville, VIC 3010, Australia
    Sex Health 2:53-62. 2005
    ..This review summarises the important role of many host genetic factors on HIV infection and highlights important variables that will need to be taken into account in evaluating effective HIV vaccines...
  56. pmc Vaccination and timing influence SIV immune escape viral dynamics in vivo
    Liyen Loh
    Department of Microbiology and Immunology, University of Melbourne, Melbourne, Victoria, Australia
    PLoS Pathog 4:e12. 2008
    ....
  57. ncbi Quantification of simian immunodeficiency virus cytotoxic T lymphocyte escape mutant viruses
    Liyen Loh
    Department of Microbiology and Immunology, University of Melbourne, Australia
    AIDS Res Hum Retroviruses 24:1067-72. 2008
    ..These data help refine our understanding of how CTL escape mutant viruses evolve...
  58. pmc In vivo fitness costs of different Gag CD8 T-cell escape mutant simian-human immunodeficiency viruses for macaques
    Liyen Loh
    Department of Microbiology and Immunology, University of Melbourne, Parkville 3010, Australia
    J Virol 81:5418-22. 2007
    ..The fitness impact of these mutations is KP9 > AF9 > KW9. These data provide insights into the differential utility of CTL in controlling viremia...
  59. pmc Robust NK cell-mediated human immunodeficiency virus (HIV)-specific antibody-dependent responses in HIV-infected subjects
    Ivan Stratov
    Department of Microbiology and Immunology, University of Melbourne, Victoria 3010, Australia
    J Virol 82:5450-9. 2008
    ..Antibody-dependent, HIV-specific NK cell recognition, involving components of both innate and adaptive immune systems, represents a potentially effective immune response to induce by vaccination...
  60. doi Thrombocytopenia is strongly associated with simian AIDS in pigtail macaques
    Sheilajen Alcantara
    Department of Microbiology and Immunology, University of Melbourne, Melbourne, Australia
    J Acquir Immune Defic Syndr 51:374-9. 2009
    ..There was however no clear correlation between the development of TCP and immune activation as measured by plasma soluble CD14. We conclude that TCP is a useful end point to analyze SIV studies in pigtail macaques...
  61. doi Safety, immunogenicity and efficacy of peptide-pulsed cellular immunotherapy in macaques
    Robert De Rose
    Department of Microbiology and Immunology, University of Melbourne, Australia
    J Med Primatol 37:69-78. 2008
    ..We recently published on the effectiveness of using ex vivo pulsing of overlapping SIV Gag 15mer peptides onto fresh peripheral blood cells in 32 SIV(mac251)-infected pigtail macaques...
  62. ncbi Anti-SIV cytolytic molecules in pigtail macaques
    Erik Rollman
    Department of Microbiology and Immunology, University of Melbourne, Melbourne 3010, Australia
    AIDS Res Hum Retroviruses 24:1127-31. 2008
    ..We also successfully studied the release of perforin and granzyme B from deregulating SIV-specific CD8 T cells by flow cytometry. These sequences and tools enable further study of the cytolytic control of SIV in pigtail macaques...
  63. pmc Analysis of pigtail macaque major histocompatibility complex class I molecules presenting immunodominant simian immunodeficiency virus epitopes
    Miranda Z Smith
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3010, Australia
    J Virol 79:684-95. 2005
    ..nemestrina MHC class I allele restricting a functionally important immunodominant SIV Gag epitope establishes a basis for studying CD8+ T-cell responses against AIDS in an important, widely available nonhuman primate species...
  64. pmc Impaired quality of the hepatitis B virus (HBV)-specific T-cell response in human immunodeficiency virus type 1-HBV coinfection
    J Judy Chang
    Department of Microbiology and Immunology, University of Melbourne, Melbourne, Australia
    J Virol 83:7649-58. 2009
    ..The quality of the HBV-specific T-cell response is impaired in the setting of HIV-1-HBV coinfection and is related to the CD4+ T-cell count...
  65. pmc Limited maintenance of vaccine-induced simian immunodeficiency virus-specific CD8 T-cell receptor clonotypes after virus challenge
    Miranda Z Smith
    Department of Microbiology and Immunology, University of Melbourne, Melbourne 3010, Australia
    J Virol 82:7357-68. 2008
    ..These findings have implications for future AIDS virus vaccine studies, which should consider the "fitness" of vaccine-induced T cells in order to generate robust responses in the face of virus exposure...
  66. ncbi The pigtail macaque MHC class I allele Mane-A*10 presents an immundominant SIV Gag epitope: identification, tetramer development and implications of immune escape and reversion
    Miranda Z Smith
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Vic, Australia
    J Med Primatol 34:282-93. 2005
    ..Mane-A*10(+) animals have lower set point SIV levels than Mane-A*10(-) animals, suggesting a significant fitness cost of escape. These studies pave the way for a more robust understanding of HIV vaccines in pigtail macaques...
  67. ncbi Rapid degranulation of NK cells following activation by HIV-specific antibodies
    Amy W Chung
    Department of Microbiology and Immunology, University of Melbourne, Melbourne, Australia
    J Immunol 182:1202-10. 2009
    ..Our studies highlight the complexity of ab-mediated NK cell activation in HIV infection, and suggest new avenues toward studying the utility of ADCC in controlling HIV infection...
  68. pmc Virus-specific T-cell immunity correlates with control of GB virus B infection in marmosets
    David J Woollard
    The Burnet Institute, G P O Box 2284, Melbourne, Victoria, Australia
    J Virol 82:3054-60. 2008
    ..These results indicate that virus-specific T-cell responses are detectable in the liver and blood of GBV-B-infected marmosets and that the clearance of GBV-B is associated with the appearance of these responses...
  69. doi A protective vaccine delivery system for in vivo T cell stimulation using nanoengineered polymer hydrogel capsules
    Amy Sexton
    Department of Microbiology and Immunology, The University of Melbourne, Parkville, Victoria, Australia
    ACS Nano 3:3391-400. 2009
    ..These results highlight the potential of nanoengineered hydrogel capsules for vaccine delivery...
  70. ncbi The utility of ADCC responses in HIV infection
    Amy Chung
    Department of Microbiology and Immunology, University of Melbourne, Victoria, 3010, Australia
    Curr HIV Res 6:515-9. 2008
    ..Nonetheless the utility of ADCC responses in preventing HIV infection requires urgent consideration...
  71. ncbi Delivery of immunotherapy with peptide-pulsed blood in macaques
    Robert De Rose
    Department of Microbiology and Immunology, University of Melbourne, 3010, Australia
    Virology 378:201-4. 2008
    ..Delivery of overlapping Gag peptides via either whole blood or PBMC is suitable for clinical evaluation...
  72. pmc Rapid viral escape at an immunodominant simian-human immunodeficiency virus cytotoxic T-lymphocyte epitope exacts a dramatic fitness cost
    Caroline S Fernandez
    Department of Microbiology and Immunology, University of Melbourne, Victoria 3010, Australia
    J Virol 79:5721-31. 2005
    ..Quantifying both the selection pressure exerted by CTL and the fitness costs of escape mutation has important implications for the development of CTL-based vaccine strategies...
  73. ncbi A paradigm for peptide vaccine delivery using viral epitopes encapsulated in degradable polymer hydrogel capsules
    Siow Feng Chong
    Centre for Nanoscience and Nanotechnology, Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia
    Biomaterials 30:5178-86. 2009
    ..The reported approach represents a novel paradigm in the delivery of peptide vaccines and other therapeutic agents...
  74. ncbi MHC class I allele frequencies in pigtail macaques of diverse origin
    Bridget F Pratt
    Department of Microbiology and Immunology, University of Melbourne, Melbourne, Victoria, 3010, Australia
    Immunogenetics 58:995-1001. 2006
    ..This broad characterization of common MHC class I alleles in more than 100 pigtail macaques further develops this animal model for the study of virus-specific CD8(+) T cell responses...
  75. pmc Reduced hepatitis B virus (HBV)-specific CD4+ T-cell responses in human immunodeficiency virus type 1-HBV-coinfected individuals receiving HBV-active antiretroviral therapy
    J Judy Chang
    Infectious Diseases Unit, Alfred Hospital, Commercial Rd, Melbourne, Victoria 3004, Australia
    J Virol 79:3038-51. 2005
    ..Therefore, HIV-1 infection has a significant and specific effect on HBV-specific T-cell immunity...
  76. ncbi The phenotype of hepatitis B virus-specific T cells differ in the liver and blood in chronic hepatitis B virus infection
    J Judy Chang
    Department of Microbiology and Immunology, University of Melbourne, Australia
    Hepatology 46:1332-40. 2007
    ....
  77. ncbi A randomized, placebo-controlled phase I trial of DNA prime, recombinant fowlpox virus boost prophylactic vaccine for HIV-1
    Anthony D Kelleher
    AIDS 20:294-7. 2006
    ..The vaccine regimen was safe, but there was no difference between vaccine (n = 18) and placebo recipients (n = 6) for Gag or Pol-specific T-cell immune responses at week 9...
  78. ncbi CD4+ T-cell subsets: what really counts in preventing HIV disease?
    Rosemarie D Mason
    Expert Rev Vaccines 7:155-8. 2008
  79. pmc CD127+CCR5+CD38+++ CD4+ Th1 effector cells are an early component of the primary immune response to vaccinia virus and precede development of interleukin-2+ memory CD4+ T cells
    John J Zaunders
    Centre for Immunology, St Vincent s Hospital, Victoria Street, Darlinghurst, NSW 2010, Australia
    J Virol 80:10151-61. 2006
    ..The current study provides important information on the differentiation of IL-2+ vaccinia virus-specific memory cells, allowing further study of antiviral effector CD4+ T cells in healthy adults and their dysfunction in HIV-1 infection...
  80. ncbi Fowlpox virus vaccines for HIV and SHIV clinical and pre-clinical trials
    Barbara E H Coupar
    CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, VIC 3220, Australia
    Vaccine 24:1378-88. 2006
    ..These data establish a preliminary safety and efficacy profile for these multigenic vaccines suggesting they are suitable for advanced development as candidate HIV vaccines...
  81. ncbi Development of a synthetic consensus sequence scrambled antigen HIV-1 vaccine designed for global use
    Scott A Thomson
    Division of Immunology and Genetics, John Curtin School of Medical Research JCSMR, Australian National University, P O Box 334, Canberra, ACT 2601, Australia
    Vaccine 23:4647-57. 2005
    ..This platform strategy could be used for other infections and cancers where T cell responses are important for protection...
  82. ncbi Comparison of whole gene and whole virus scrambled antigen approaches for DNA prime and fowlpox virus boost HIV type 1 vaccine regimens in macaques
    Joko Pamungkas
    Primate Research Center, Bogor Agricultural University, PSSP IPB, Bogor, Indonesia 16151
    AIDS Res Hum Retroviruses 21:292-300. 2005
    ..Further refinements are planned with alternative vector strategies to evaluate the potential of the SAVINE technology...
  83. pmc CD4+ target cell availability determines the dynamics of immune escape and reversion in vivo
    Janka Petravic
    Complex Systems in Biology Group, Centre for Vascular Research, University of New South Wales, Kensington, NSW 2052, Australia
    J Virol 82:4091-101. 2008
    ..This has important implications for comparative studies of immune escape and reversion in different infections and for identifying epitopes with high fitness cost for use as vaccine targets...