B E Kemp

Summary

Affiliation: University of Melbourne
Country: Australia

Publications

  1. ncbi Bateman domains and adenosine derivatives form a binding contract
    Bruce E Kemp
    St Vincent s Institute of Medical Research, Fitzroy, Victoria, Australia
    J Clin Invest 113:182-4. 2004
  2. ncbi AMP-activated protein kinase, super metabolic regulator
    B E Kemp
    St Vincent s Institute of Medical Research, and Department of Medicine, University of Melbourne, 41 Victoria Parade, Fitzroy, VIC 3065, Australia
    Biochem Soc Trans 31:162-8. 2003
  3. ncbi Regulation and crystallization of phosphorylated and dephosphorylated forms of truncated dimeric phenylalanine hydroxylase
    B Kobe
    St Vincent s Institute of Medical Research, Fitzroy, Victoria, Australia
    Protein Sci 6:1352-7. 1997
  4. ncbi Posttranslational modifications of the 5'-AMP-activated protein kinase beta1 subunit
    K I Mitchelhill
    St Vincent s Institute of Medical Research, 41 Victoria Parade, Fitzroy, Victoria 3065 Australia
    J Biol Chem 272:24475-9. 1997
  5. ncbi AMP-activated protein kinase isoenzyme family: subunit structure and chromosomal location
    D Stapleton
    St Vincent s Institute of Medical Research, St Vincent s Hospital, Fitzroy, Victoria, Australia
    FEBS Lett 409:452-6. 1997
  6. ncbi Expression of the AMP-activated protein kinase beta1 and beta2 subunits in skeletal muscle
    Z Chen
    St Vincent s Institute of Medical Research, St Vincent s Hospital, 41 Victoria Parade, Fitzroy, Vic, Australia
    FEBS Lett 460:343-8. 1999
  7. ncbi Crystallization of a trimeric human T cell leukemia virus type 1 gp21 ectodomain fragment as a chimera with maltose-binding protein
    R J Center
    St Vincent s Institute of Medical Research, Fitzroy, Australia
    Protein Sci 7:1612-9. 1998
  8. ncbi Dealing with energy demand: the AMP-activated protein kinase
    B E Kemp
    St Vincent s Institute of Medical Research, Fitzroy, Victoria
    Trends Biochem Sci 24:22-5. 1999
  9. ncbi AMPK signaling in contracting human skeletal muscle: acetyl-CoA carboxylase and NO synthase phosphorylation
    Z P Chen
    St Vincent s Institute of Medical Research, St Vincent s Hospital, Fitzroy, Victoria 3065, Australia
    Am J Physiol Endocrinol Metab 279:E1202-6. 2000
  10. ncbi Mammalian AMP-activated protein kinase subfamily
    D Stapleton
    St Vincent s Institute of Medical Research, Fitzroy, Victoria, Australia
    J Biol Chem 271:611-4. 1996

Collaborators

Detail Information

Publications95

  1. ncbi Bateman domains and adenosine derivatives form a binding contract
    Bruce E Kemp
    St Vincent s Institute of Medical Research, Fitzroy, Victoria, Australia
    J Clin Invest 113:182-4. 2004
    ....
  2. ncbi AMP-activated protein kinase, super metabolic regulator
    B E Kemp
    St Vincent s Institute of Medical Research, and Department of Medicine, University of Melbourne, 41 Victoria Parade, Fitzroy, VIC 3065, Australia
    Biochem Soc Trans 31:162-8. 2003
    ....
  3. ncbi Regulation and crystallization of phosphorylated and dephosphorylated forms of truncated dimeric phenylalanine hydroxylase
    B Kobe
    St Vincent s Institute of Medical Research, Fitzroy, Victoria, Australia
    Protein Sci 6:1352-7. 1997
    ..Truncation also facilitates crystallization of both phosphorylated and dephosphorylated forms of the enzyme...
  4. ncbi Posttranslational modifications of the 5'-AMP-activated protein kinase beta1 subunit
    K I Mitchelhill
    St Vincent s Institute of Medical Research, 41 Victoria Parade, Fitzroy, Victoria 3065 Australia
    J Biol Chem 272:24475-9. 1997
    ..Based on labeling studies in transfected cells, we conclude that alpha1 Thr172 is a major, although not exclusive, site of both basal and stimulated alpha1 phosphorylation by an upstream AMPK kinase...
  5. ncbi AMP-activated protein kinase isoenzyme family: subunit structure and chromosomal location
    D Stapleton
    St Vincent s Institute of Medical Research, St Vincent s Hospital, Fitzroy, Victoria, Australia
    FEBS Lett 409:452-6. 1997
    ..Human chromosomal localizations were determined for AMPK-alpha1 (5p11-p14), AMPK-beta1 (12q24.1-24.3) and AMPK-gamma1 (12q12-q14), respectively...
  6. ncbi Expression of the AMP-activated protein kinase beta1 and beta2 subunits in skeletal muscle
    Z Chen
    St Vincent s Institute of Medical Research, St Vincent s Hospital, 41 Victoria Parade, Fitzroy, Vic, Australia
    FEBS Lett 460:343-8. 1999
    ..Sequence analysis revealed that glycogen synthase, a known AMPK substrate, co-immunoprecipitated with the AMPK alpha2beta2gamma1 complex...
  7. ncbi Crystallization of a trimeric human T cell leukemia virus type 1 gp21 ectodomain fragment as a chimera with maltose-binding protein
    R J Center
    St Vincent s Institute of Medical Research, Fitzroy, Australia
    Protein Sci 7:1612-9. 1998
    ..The envelope protein precursor, gp62, is likewise trimeric when expressed in mammalian cells. Our results suggest that MBP may have a general application for the crystallization of proteins containing N-terminal alpha-helical sequences...
  8. ncbi Dealing with energy demand: the AMP-activated protein kinase
    B E Kemp
    St Vincent s Institute of Medical Research, Fitzroy, Victoria
    Trends Biochem Sci 24:22-5. 1999
    ..The AMPK yeast homolog, Snf1p, plays a major role in adaption to glucose deprivation. In mammals, AMPK also has diverse roles that extend from energy metabolism through to transcriptional control...
  9. ncbi AMPK signaling in contracting human skeletal muscle: acetyl-CoA carboxylase and NO synthase phosphorylation
    Z P Chen
    St Vincent s Institute of Medical Research, St Vincent s Hospital, Fitzroy, Victoria 3065, Australia
    Am J Physiol Endocrinol Metab 279:E1202-6. 2000
    ....
  10. ncbi Mammalian AMP-activated protein kinase subfamily
    D Stapleton
    St Vincent s Institute of Medical Research, Fitzroy, Victoria, Australia
    J Biol Chem 271:611-4. 1996
    ..5-kilobase mRNA and is most prominent in skeletal muscle, heart, and liver. Isoforms of the beta and gamma subunits present in the human genome sequence reveal that the AMPK consists of a family of isoenzymes...
  11. ncbi Mammalian 5'-AMP-activated protein kinase non-catalytic subunits are homologs of proteins that interact with yeast Snf1 protein kinase
    D Stapleton
    St Vincent s Institute of Medical Research, Fitzroy, Victoria, Australia
    J Biol Chem 269:29343-6. 1994
    ....
  12. ncbi Reduced AMP-activated protein kinase activity in mouse skeletal muscle does not exacerbate the development of insulin resistance with obesity
    S Beck Jørgensen
    St Vincent s Institute of Medical Research and Department of Medicine, University of Melbourne, Fitzroy, Vic, Australia
    Diabetologia 52:2395-404. 2009
    ..We investigated whether reduced levels of muscle AMPK promote lipid accumulation and insulin resistance during high-fat feeding...
  13. ncbi Interaction of the recombinant S100A1 protein with twitchin kinase, and comparison with other Ca2+-binding proteins
    J Heierhorst
    St Vincent s Institute of Medical Research, Fitzroy, Victoria, Australia
    Eur J Biochem 249:127-33. 1997
    ....
  14. ncbi Substrate specificity and inhibitor sensitivity of Ca2+/S100-dependent twitchin kinases
    J Heierhorst
    St Vincent s Institute of Medical Research, Fitzroy, Victoria, Australia
    Eur J Biochem 242:454-9. 1996
    ..The twitchin kinase S100A1(2)-binding site can also bind Ca2+/calmodulin but neither kinase is activated by calmodulin. The data provide a functional basis for the ongoing crystallographic study of twitchin kinase fragments...
  15. ncbi Differential attenuation of AMPK activation during acute exercise following exercise training or AICAR treatment
    G K McConell
    Department of Physiology, The University of Melbourne, Melbourne, Victoria, Australia
    J Appl Physiol 105:1422-7. 2008
    ..In conclusion, 10 days of AICAR administration substantially mimics the effect of 10 days training on attenuating skeletal muscle AMPK activation in response to subsequent exercise...
  16. ncbi Coordinated control of endothelial nitric-oxide synthase phosphorylation by protein kinase C and the cAMP-dependent protein kinase
    B J Michell
    St. Vincent's Institute of Medical Research, St. Vincent's Hospital, 41 Victoria Parade, Fitzroy, Victoria 3065, Australia
    J Biol Chem 276:17625-8. 2001
    ..These results show that regulation of eNOS activity involves coordinated signaling through Ser-1177 and Thr-495 by multiple protein kinases and phosphatases...
  17. ncbi Effect of exercise intensity and hypoxia on skeletal muscle AMPK signaling and substrate metabolism in humans
    G D Wadley
    Department of Physiology, The University of Melbourne, Parkville, Victoria, Australia
    Am J Physiol Endocrinol Metab 290:E694-702. 2006
    ....
  18. ncbi Synapsins as major neuronal Ca2+/S100A1-interacting proteins
    J Heierhorst
    St Vincent s Institute of Medical Research, 41 Victoria Parade, Fitzroy, VIC 3065, Australia
    Biochem J 344:577-83. 1999
    ..Double-labelling confocal immunofluorescence microscopy demonstrated that synapsins and S100A1 are both present in the soma and neurites of PC12 cells, indicating their potential to interact in neurons in vivo...
  19. ncbi Crystal structure of human T cell leukemia virus type 1 gp21 ectodomain crystallized as a maltose-binding protein chimera reveals structural evolution of retroviral transmembrane proteins
    B Kobe
    St Vincent s Institute of Medical Research, 41 Victoria Parade, Fitzroy, Victoria 3065, Australia
    Proc Natl Acad Sci U S A 96:4319-24. 1999
    ..The structure of gp21 implies that the N-terminal fusion peptide is in close proximity to the C-terminal transmembrane domain and likely represents a postfusion conformation...
  20. ncbi Active site-directed protein regulation
    B Kobe
    St Vincent s Institute of Medical Research, Fitzroy, Victoria, Australia
    Nature 402:373-6. 1999
    ..Intrasteric regulation, directed at the active site and thus the counterpart of allosteric control, is now emerging as an important regulatory mechanism...
  21. ncbi Phosphorylation at the cyclin-dependent kinases site (Thr85) of parathyroid hormone-related protein negatively regulates its nuclear localization
    M H Lam
    St Vincent s Institute of Medical Research and the Department of Medicine, The University of Melbourne, 41 Victoria Parade, Fitzroy, Victoria 3065, Australia
    J Biol Chem 274:18559-66. 1999
    ..Combined, the data demonstrate that the intracellular localization of PTHrP is phosphorylation- and cell cycle-dependent, and such control further supports a potential intracellular role (10,34,35) for PTHrP...
  22. ncbi A cardiolipin-activated protein kinase from rat liver structurally distinct from the protein kinases C
    N A Morrice
    Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia
    J Biol Chem 269:20040-6. 1994
    ..It was concluded that PAK-1 is a phospholipid-regulated protein kinase with a primary structure, substrate specificity, and mechanism of regulation in vitro distinct from those of any known member of the protein kinase C superfamily...
  23. ncbi Structural basis of autoregulation of phenylalanine hydroxylase
    B Kobe
    St Vincent s Institute of Medical Research, Fitzroy, Victoria, Australia
    Nat Struct Biol 6:442-8. 1999
    ....
  24. ncbi Casein kinase II beta-subunit inhibits the activity of the catalytic alpha-subunit in the absence of salt
    T Tiganis
    St Vincent s Institute of Medical Research, Fitzroy, Vic, Australia
    Biochim Biophys Acta 1203:282-9. 1993
    ..Since the kcat for the Drosophila alpha-subunit is approx. 3-fold greater than the Drosophila CKII measured in the absence of added salt these results indicate that the beta-subunit acts primarily as an inhibitory subunit...
  25. ncbi The protein-tyrosine phosphatase TCPTP regulates epidermal growth factor receptor-mediated and phosphatidylinositol 3-kinase-dependent signaling
    T Tiganis
    St Vincent s Institute of Medical Research, Fitzroy, Victoria 3065, Australia
    J Biol Chem 274:27768-75. 1999
    ..Thus, TC45 may serve as a negative regulator of growth factor or integrin-induced, EGF receptor-mediated PI 3-kinase signaling...
  26. ncbi Mammalian AMP-activated protein kinase shares structural and functional homology with the catalytic domain of yeast Snf1 protein kinase
    K I Mitchelhill
    St Vincent s Institute of Medical Research, Fitzroy, Victoria, Australia
    J Biol Chem 269:2361-4. 1994
    ....
  27. ncbi Examination of 'lipotoxicity' in skeletal muscle of high-fat fed and ob/ob mice
    S M Turpin
    St Vincent s Institute of Medical Research and the Department of Medicine, University of Melbourne, Fitzroy, Australia
    J Physiol 587:1593-605. 2009
    ..A broad transcriptional suppression of pro-apoptotic proteins may explain this resistance to lipid-induced cell death in skeletal muscle...
  28. ncbi Structure and function of AMP-activated protein kinase
    J S Oakhill
    St Vincent s Institute and Department of Medicine University of Melbourne, Fitzroy, Victoria, Australia
    Acta Physiol (Oxf) 196:3-14. 2009
    ..Two direct activating drugs have been reported for AMPK, the thienopyridone compound A769662 and PTI, which may activate by distinct mechanisms...
  29. ncbi Proteomic-based identification of haptoglobin-1 precursor as a novel circulating biomarker of ovarian cancer
    N Ahmed
    Gynaecological Cancer Research Centre, Royal Women s Hospital, 132 Grattan Street, Carlton, Victoria 3053, Australia
    Br J Cancer 91:129-40. 2004
    ..These data highlight the need to assess circulating concentration of HAP1 in the serum of ovarian cancer patients and evaluate its potential as a biomarker in the early diagnosis of ovarian cancer...
  30. ncbi Human immunodeficiency virus type 1 envelope glycoprotein oligomerization requires the gp41 amphipathic alpha-helical/leucine zipper-like sequence
    P Poumbourios
    St Vincent s Institute of Medical Research, Fitzroy, Victoria, Australia
    J Virol 71:2041-9. 1997
    ..These results indicate that the gp41 amphipathic alpha-helix 4-3 hydrophobic repeat sequence plays a central role in HIV-1 envelope glycoprotein oligomerization and fusion function...
  31. ncbi Calcitonin receptors of human osteoclastoma
    G C Nicholson
    University of Melbourne, Department of Medicine, Repatriation General Hospital, Heidelberg, Victoria, Australia
    Horm Metab Res 19:585-9. 1987
    ..The multinucleate giant cells (osteoclasts) of human osteoclastomas possess large number of CT receptors which exhibit the same binding kinetics and apparent Mr as those of other CT target cells...
  32. ncbi Cellular distribution and developmental expression of AMP-activated protein kinase isoforms in mouse central nervous system
    A M Turnley
    The Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia
    J Neurochem 72:1707-16. 1999
    ....
  33. ncbi Aminoguanidine and ramipril prevent diabetes-induced increases in protein kinase C activity in glomeruli, retina and mesenteric artery
    T M Osicka
    Endocrine Unit, Department of Medicine, University of Melbourne, Austin and Repatriation Medical Centre, Heidelberg, Victoria 3084, Australia
    Clin Sci (Lond) 100:249-57. 2001
    ..These results suggest that the organ-protective effects of insulin, aminoguanidine and ramipril in diabetes may be mediated, at least in part, through the differential inhibition of PKC activity in various tissues...
  34. ncbi N-terminal residues 105-117 of HIV-1 gp120 are not involved in CD4 binding
    W El Ahmar
    St Vincent s Institute of Medical Research, Fitzroy, Victoria, Australia
    AIDS Res Hum Retroviruses 7:855-8. 1991
    ..The reported loss of CD4 binding ability, resulting from the deletion in this region of gp120, is likely to be due to indirect structural changes in gp120 rather than representing an integral part of the CD4 binding domain...
  35. ncbi A potent inhibitor of osteoclastic bone resorption within a highly conserved pentapeptide region of parathyroid hormone-related protein; PTHrP[107-111]
    A J Fenton
    Dept of Medicine, University of Melbourne, Geelong Hospital, Victoria, Australia
    Endocrinology 129:3424-6. 1991
    ..This data provides the basis for the development of further analogs with potential therapeutic use in disorders associated with increased osteoclastic bone resorption...
  36. ncbi Reduced glycogen availability is associated with increased AMPKalpha2 activity, nuclear AMPKalpha2 protein abundance, and GLUT4 mRNA expression in contracting human skeletal muscle
    Gregory R Steinberg
    Vincent s Institute and Department of Medicine, University of Melbourne, Fitzroy, Victoria, Australia
    Appl Physiol Nutr Metab 31:302-12. 2006
    ..We suggest that increased activation of AMPK alpha2 under conditions of low muscle glycogen enhances AMPK alpha2 nuclear translocation and increases GLUT4 mRNA expression in response to exercise in human skeletal muscle...
  37. ncbi Ciliary neurotrophic factor suppresses hypothalamic AMP-kinase signaling in leptin-resistant obese mice
    Gregory R Steinberg
    St Vincent s Institute, 9 Princes Street, Fitzroy, Victoria 3065, Australia
    Endocrinology 147:3906-14. 2006
    ..A HFD blunted leptin but not CNTF effects on AMPK signaling in the ARC and PVN. In summary, these data demonstrate that CNTF(Ax15) bypasses diet-induced leptin resistance to reduce hypothalamic AMPK activity...
  38. ncbi Carbohydrate ingestion does not alter skeletal muscle AMPK signaling during exercise in humans
    Robert S Lee-Young
    Dept of Physiology, The University of Melbourne, Carlton, Victoria, Australia
    Am J Physiol Endocrinol Metab 291:E566-73. 2006
    ....
  39. ncbi Fatty acids stimulate AMP-activated protein kinase and enhance fatty acid oxidation in L6 myotubes
    Matthew J Watt
    Cellular and Molecular Metabolism Laboratory, School of Medical Sciences, RMIT University, Victoria, Australia
    J Physiol 574:139-47. 2006
    ....
  40. ncbi CNTF reverses obesity-induced insulin resistance by activating skeletal muscle AMPK
    Matthew J Watt
    Cellular and Molecular Metabolism Laboratory, School of Medical Sciences, Royal Melbourne Institute of Technology, PO Box 71, Bundoora, 3083, Australia
    Nat Med 12:541-8. 2006
    ....
  41. ncbi Short-term exercise training in humans reduces AMPK signalling during prolonged exercise independent of muscle glycogen
    Glenn K McConell
    Department of Physiology, University of Melbourne, Parkville, Victoria, Australia
    J Physiol 568:665-76. 2005
    ..It appears that muscle glycogen is not an important regulator of AMPK activation during exercise in humans when exercise is begun with normal or high muscle glycogen levels...
  42. ncbi Phosphorylation of neuronal and endothelial nitric oxide synthase in the kidney with high and low salt diets
    Peter F Mount
    The Austin Research Institute, Austin Hospital, Heidelberg, Australia
    Nephron Physiol 102:p36-50. 2006
    ..We hypothesized that activating or inhibitory phosphorylation of neuronal and endothelial nitric oxide synthase (nNOS, eNOS) regulates renal NO production in response to altered dietary salt...
  43. ncbi Structural basis for glycogen recognition by AMP-activated protein kinase
    Galina Polekhina
    St. Vincent's Institute of Medical Research, 41 Victoria Parade, Fitzroy, Victoria 3065, Australia
    Structure 13:1453-62. 2005
    ..Modeling suggests that this binding pocket enables AMPK to interact with glycogen anywhere across the carbohydrate's helical surface...
  44. ncbi Chronic rosiglitazone treatment restores AMPKalpha2 activity in insulin-resistant rat skeletal muscle
    Sarah J Lessard
    School of Medical Sciences, RMIT University, Melbourne, Victoria, Australia
    Am J Physiol Endocrinol Metab 290:E251-7. 2006
    ..ACC phosphorylation was decreased with obesity (P < 0.05) but restored to the level of lean controls with RSG treatment. Our data demonstrate that RSG restores AMPK signaling in skeletal muscle of insulin-resistant obese Zucker rats...
  45. ncbi Regulation of HSL serine phosphorylation in skeletal muscle and adipose tissue
    Matthew J Watt
    Cellular and Molecular Metabolism Laboratory, School of Medical Sciences, RMIT University, PO Box 71, Bundoora 3083, Victoria, Australia
    Am J Physiol Endocrinol Metab 290:E500-8. 2006
    ..Taken together, these data indicate that HSL activity is maintained in the face of AMPK activation as a result of elevated HSL Ser(660) phosphorylation in adipose tissue but not skeletal muscle...
  46. ncbi The suppressor of cytokine signaling 3 inhibits leptin activation of AMP-kinase in cultured skeletal muscle of obese humans
    Gregory R Steinberg
    St Vincent s Institute, 9 Princes Street, Fitzroy, Victoria 3065, Australia
    J Clin Endocrinol Metab 91:3592-7. 2006
    ..Human obesity is associated with leptin resistance, and recent studies have demonstrated that hypothalamic expression of the suppressors of cytokine signaling 3 (SOCS3) regulates leptin sensitivity in rodents...
  47. ncbi Adrenergic regulation of HSL serine phosphorylation and activity in human skeletal muscle during the onset of exercise
    Jason L Talanian
    Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1
    Am J Physiol Regul Integr Comp Physiol 291:R1094-9. 2006
    ..This adrenergic stimulation contributes to the increase in HSL activity that occurs in human skeletal muscle in the first minute of exercise at 65% and 90% VO2 peak...
  48. ncbi Metabolic remodeling in adipocytes promotes ciliary neurotrophic factor-mediated fat loss in obesity
    Seamus Crowe
    St Vincent s Institute of Medical Research and the Department of Medicine, The University of Melbourne, Fitzroy, Victoria 3065, Australia
    Endocrinology 149:2546-56. 2008
    ..We conclude that CNTF reprograms adipose tissue to promote mitochondrial biogenesis, enhancing oxidative capacity and reducing lipogenic capacity, thereby resulting in triglyceride loss...
  49. ncbi Increased glycogen stores due to gamma-AMPK overexpression protects against ischemia and reperfusion damage
    Michal Ofir
    The Cardiac Research Laboratory, Felsenstein Medical Research Center, Rabin Medical Center, Tel Aviv University, Petah Tikva 49100, Israel
    Biochem Pharmacol 75:1482-91. 2008
    ..These findings demonstrate the potential of heart protection from ischemia and reperfusion injury through metabolic manipulation increasing the level and utilization of myocardial glycogen...
  50. ncbi AMP-activated protein kinase regulates GLUT4 transcription by phosphorylating histone deacetylase 5
    Sean L McGee
    Department of Physiology, The University of Melbourne, 3010, Australia
    Diabetes 57:860-7. 2008
    ..The molecular mechanisms regulating skeletal muscle GLUT4 expression remain to be elucidated. The purpose of this study was to examine these mechanisms...
  51. ncbi AMP-activated protein kinase subunit interactions: beta1:gamma1 association requires beta1 Thr-263 and Tyr-267
    Tristan J Iseli
    St Vincent s Institute, Fitzroy, Victoria 3065, Australia
    J Biol Chem 283:4799-807. 2008
    ..Moreover, using alanine mutagenesis we show that beta1 Thr-263 and Tyr-267 are required for betagamma association but not alphabeta association...
  52. ncbi Regulation of the renal-specific Na+-K+-2Cl- co-transporter NKCC2 by AMP-activated protein kinase (AMPK)
    Scott A Fraser
    The Burnet Research Institute, Austin Health, Studley Road, Heidelberg, Victoria 3084, Australia
    Biochem J 405:85-93. 2007
    ..Moreover, the metabolic-sensing kinase, AMPK, is able to phosphorylate this site, potentially linking the cellular energy state with changes in co-transporter activity...
  53. ncbi Dysregulation of muscle lipid metabolism in rats selectively bred for low aerobic running capacity
    Fiona J Spargo
    Exercise Metabolism Group, School of Medical Sciences, Australia
    Am J Physiol Endocrinol Metab 292:E1631-6. 2007
    ..In conclusion, rats with low intrinsic aerobic capacity demonstrate abnormalities in lipid-handling capacity. These disruptions may, in part, be responsible for the increased risk of metabolic disorders observed in this phenotype...
  54. ncbi Differential regulation of adiponectin receptor gene expression by adiponectin and leptin in myotubes derived from obese and diabetic individuals
    Andrew J McAinch
    School of Exercise and Nutrition Sciences, Deakin University, 221 Burwood Highway, Burwood, Victoria 3125, Australia
    Obesity (Silver Spring) 14:1898-904. 2006
    ..The incapacity of skeletal muscle of obese and diabetic individuals to respond to exogenous adiponectin and leptin may be further suppressed as a result of impaired regulation of the AdipoR1 gene...
  55. ncbi Genetic model for the chronic activation of skeletal muscle AMP-activated protein kinase leads to glycogen accumulation
    Laura Barré
    Department of Medicine, Dartmouth Medical School, Dartmouth College, Hanover, NH 03755 3833, USA
    Am J Physiol Endocrinol Metab 292:E802-11. 2007
    ..Transgene expression of this mutant AMPKgamma1 subunit may provide a useful model for the chronic activation of AMPK in other tissues to clarify its multiple roles in the regulation of metabolism and other physiological processes...
  56. ncbi AMP-activated protein kinase impairs endothelial actin cytoskeleton assembly by phosphorylating vasodilator-stimulated phosphoprotein
    Constanze Blume
    Institute for Clinical Biochemistry and Pathobiochemistry, Julius Maximilians University Würzburg, Josef Schneider Strasse 2, D 97080 Wurzburg, Germany
    J Biol Chem 282:4601-12. 2007
    ..These findings suggest that VASP is a new AMPK substrate, that VASP Thr-278 phosphorylation translates metabolic signals into actin cytoskeleton rearrangements, and that this signaling system becomes down-regulated in diabetic vessels...
  57. ncbi Regulation of AMP-activated protein kinase by multisite phosphorylation in response to agents that elevate cellular cAMP
    Rebecca L Hurley
    Department of Medicine and Biochemistry, Dartmouth Medical School, Remsen 322, N College St, Hanover, NH 03755, USA
    J Biol Chem 281:36662-72. 2006
    ....
  58. ncbi Interleukin-6 increases insulin-stimulated glucose disposal in humans and glucose uptake and fatty acid oxidation in vitro via AMP-activated protein kinase
    Andrew L Carey
    Cellular and Molecular Metabolism Laboratory, School of Medical Sciences, Royal Melbourne Institute of Technology RMIT University, Australia
    Diabetes 55:2688-97. 2006
    ..Our results demonstrate that acute IL-6 treatment enhances insulin-stimulated glucose disposal in humans in vivo, while the effects of IL-6 on glucose and fatty acid metabolism in vitro appear to be mediated by AMPK...
  59. ncbi AMP-activated protein kinase--the fat controller of the energy railroad
    Gregory R Steinberg
    St. Vincent's Institute, 41 Victoria Pd, Fitzroy, 3065 Victoria, Australia
    Can J Physiol Pharmacol 84:655-65. 2006
    ..As such, it can be considered to be the fat controller of the energy railroad. It is thought that AMPK may be a major mediator of the health benefits of exercise in mitigating the development of obesity and age-onset diseases...
  60. ncbi The Ca2+/calmodulin-dependent protein kinase kinases are AMP-activated protein kinase kinases
    Rebecca L Hurley
    Departments of Medicine and Biochemistry, Dartmouth Medical School and the Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA
    J Biol Chem 280:29060-6. 2005
    ..These data indicate that the CaMKKs function in intact cells as AMPKKs, predicting wider roles for these kinases in regulating AMPK activity in vivo...
  61. ncbi Acute renal ischemia rapidly activates the energy sensor AMPK but does not increase phosphorylation of eNOS-Ser1177
    Peter F Mount
    Austin Research Institute, Austin Health, University of Melbourne, Heidelberg 3084, Victoria, Australia
    Am J Physiol Renal Physiol 289:F1103-15. 2005
    ..The substrates it phosphorylates, however, are different from those in other organs such as the heart...
  62. ncbi A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases
    Christopher Belham
    Department of Molecular Biology and Medical Services, Massachusetts General Hospital, and Harvard Medical School, Boston, Massachusetts 02114, USA
    J Biol Chem 278:34897-909. 2003
    ..These findings support the conclusion that Nercc1/Nek9 and Nek6 represent a novel cascade of mitotic NIMA family protein kinases whose combined function is important for mitotic progression...
  63. ncbi AMP-activated protein kinase (AMPK) regulates the insulin-induced activation of the nitric oxide synthase in human platelets
    Ingrid Fleming
    Institut für Kardiovaskuläre Physiologie, Klinikum der J W Goethe Universitat, Theodor Stern Kai 7, D 60596 Frankfurt Main, Germany
    Thromb Haemost 90:863-71. 2003
    ....
  64. ncbi Effect of exercise intensity on skeletal muscle AMPK signaling in humans
    Zhi Ping Chen
    St Vincent s Institute of Medical Research, University of Melbourne, Fitzroy, Victoria, Australia
    Diabetes 52:2205-12. 2003
    ..These results indicate that ACC beta phosphorylation is especially sensitive to exercise and tightly coupled to AMPK signaling and that AMPK activation does not depend on AMPK kinase activation during exercise...
  65. ncbi Identification of a parathyroid hormone in the fish Fugu rubripes
    Janine A Danks
    St Vincent s Institute of Medical Research, Fitzroy, Victoria, Australia
    J Bone Miner Res 18:1326-31. 2003
    ..1 cells. Rabbit polyclonal antisera specific for N-terminal human PTHrP and one rabbit polyclonal antiserum specific for N terminus hPTH were used to test the cross-reactivity with fPTH(1-34) in immunoblots...
  66. ncbi Shear stress stimulates phosphorylation of eNOS at Ser(635) by a protein kinase A-dependent mechanism
    Yong Chool Boo
    GA Inst Tech, Atlanta
    Am J Physiol Heart Circ Physiol 283:H1819-28. 2002
    ..Phosphorylation of Ser(635) may play an important role in chronic regulation of eNOS in response to mechanical and humoral stimuli...
  67. ncbi Skeletal muscle basal AMP-activated protein kinase activity is chronically elevated in alloxan-diabetic dogs: impact of exercise
    Michael J Christopher
    Dept of Endocrinology and Diabetes, St Vincent s Hospital Melbourne, 35 Victoria Parade, Fitzroy 3065, Victoria, Australia
    J Appl Physiol 95:1523-30. 2003
    ....
  68. ncbi AMPK beta subunit targets metabolic stress sensing to glycogen
    Galina Polekhina
    St. Vincent's Institute of Medical Research, University of Melbourne, 41 Victoria Parade, Fitzroy, Australia
    Curr Biol 13:867-71. 2003
    ..Glycogen binding provides an architectural link between AMPK and a major cellular energy store and juxtaposes AMPK to glycogen bound phosphatases...
  69. ncbi Exercise increases nuclear AMPK alpha2 in human skeletal muscle
    Sean L McGee
    Exercise, Muscle and Metabolism Unit, School of Health Sciences, Deakin University, Burwood, Australia
    Diabetes 52:926-8. 2003
    ..024). There was no change in whole-cell AMPK alpha(2) content or AMPK alpha(2) mRNA abundance. These results suggest that nuclear translocation of AMPK might mediate the effects of exercise on skeletal muscle gene and protein expression...
  70. ncbi Compensatory phosphorylation and protein-protein interactions revealed by loss of function and gain of function mutants of multiple serine phosphorylation sites in endothelial nitric-oxide synthase
    Philip M Bauer
    Department of Pharmacology and Vascular Cell Signaling and Therapeutics Program, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut 06536, USA
    J Biol Chem 278:14841-9. 2003
    ..Ser-617 may not be important for directly regulating NO release but is important as a modulator of phosphorylation at other sites and protein-protein interactions...
  71. ncbi Regulation of 5'AMP-activated protein kinase activity and substrate utilization in exercising human skeletal muscle
    Jorgen F P Wojtaszewski
    Department of Human Physiology, Institute of Exercise and Sport Sciences, University of Copenhagen, 2100 Copenhagen, Denmark
    Am J Physiol Endocrinol Metab 284:E813-22. 2003
    ..It is concluded that muscle AMPK activity and ACCbeta Ser(221) phosphorylation at rest and during exercise are sensitive to the fuel status of the muscle. During exercise, this dependence may in part be mediated by humoral factors...
  72. ncbi A possible linkage between AMP-activated protein kinase (AMPK) and mammalian target of rapamycin (mTOR) signalling pathway
    Naoki Kimura
    Biosignal Research Center, Kobe University, Kobe 657 8501, Japan
    Genes Cells 8:65-79. 2003
    ..AMP-activated protein kinase (AMPK) modulates metabolism in response to energy demand by responding to changes in AMP...
  73. ncbi Intrasteric control of AMPK via the gamma1 subunit AMP allosteric regulatory site
    Julian Adams
    St. Vincent's Institute of Medical Research, Fitzroy, Victoria 3065, Australia
    Protein Sci 13:155-65. 2004
    ..We propose that ATP acts as an intrasteric inhibitor by bridging the alpha and gamma subunits and that AMP functions to derepress AMPK activity...
  74. ncbi Reduced NOS3 phosphorylation mediates reduced NO/cGMP signaling in mesenteric arteries of deoxycorticosterone acetate-salt hypertensive rats
    Jennifer M Sasser
    Department of Pharmacology, Medical College of Georgia, Augusta 30912, USA
    Hypertension 43:1080-5. 2004
    ..These findings indicate that diminished NO/cGMP signaling in mesenteric arteries from DOCA-salt rats is caused by reduced phosphorylation of NOS3 at serine 1179 and serine 635, rather than NO scavenging by superoxide...
  75. ncbi The T-cell protein tyrosine phosphatase is phosphorylated on Ser-304 by cyclin-dependent protein kinases in mitosis
    Patricia Bukczynska
    Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Victoria 3800, Australia
    Biochem J 380:939-49. 2004
    ..In addition, recombinant CDKs, but not the Polo-like kinase Plk1, phosphorylated Ser-304 in vitro. Our studies identify Ser-304 as a major phosphorylation site in human TCPTP, and the TC45 variant as a novel mitotic CDK substrate...
  76. ncbi Role of 5'AMP-activated protein kinase in glycogen synthase activity and glucose utilization: insights from patients with McArdle's disease
    Jakob N Nielsen
    Copenhagen Muscle Research Centre, Department of Human Physiology, Institute of Exercise and Sport Sciences, University of Copenhagen, Denmark
    J Physiol 541:979-89. 2002
    ....
  77. ncbi Impaired activation of AMP-kinase and fatty acid oxidation by globular adiponectin in cultured human skeletal muscle of obese type 2 diabetics
    Michael B Chen
    St Vincent s Institute, 9 Princes Street, Fitzroy, Victoria 3065, Australia
    J Clin Endocrinol Metab 90:3665-72. 2005
    ..These data suggest that reduced activation of AMPK by gAD in obese and obese type 2 diabetic subjects is not caused by reduced adiponectin receptor expression but that aspects downstream of the receptor may inhibit AMPK signaling...
  78. ncbi Prevailing hyperglycemia is critical in the regulation of glucose metabolism during exercise in poorly controlled alloxan-diabetic dogs
    Michael J Christopher
    Department of Endocrinology and Diabetes, St. Vincent's Hospital Melbourne, 35 Victoria Parade, Fitzroy 3065, Victoria, Australia
    J Appl Physiol 98:930-9. 2005
    ..During phlorizin-induced NGD, increments in both Rd(tissue) and GF are impaired due to a diminished fuel supply from plasma glucose and a sustained reduction in increments of MCRg...
  79. ncbi AMP-activated protein kinase beta subunit tethers alpha and gamma subunits via its C-terminal sequence (186-270)
    Tristan J Iseli
    St Vincent s Institute, University of Melbourne, Victoria, Australia
    J Biol Chem 280:13395-400. 2005
    ..The conserved C-terminal 85-residue sequence of the beta subunit (90% between beta1 and beta2) is the primary alphagamma binding sequence responsible for the formation of the AMPK alphabetagamma heterotrimer...
  80. ncbi Protein kinase C beta inhibition attenuates the progression of experimental diabetic nephropathy in the presence of continued hypertension
    Darren J Kelly
    Department of Medicine, University of Melbourne, St Vincent s Hospital, Fitzroy, Victoria, Australia
    Diabetes 52:512-8. 2003
    ..In vivo inhibition of PKC beta with LY333531 led to a reduction in albuminuria, structural injury, and TGF-beta expression, despite continued hypertension and hyperglycemia...
  81. ncbi Effect of exercise on protein kinase C activity and localization in human skeletal muscle
    Adam J Rose
    School of Exercise and Nutrition Sciences, Deakin University, Burwood, Victoria 3125, Australia
    J Physiol 561:861-70. 2004
    ..These data indicate that aPKC, but not cPKC or PKC, are activated by exercise in contracting muscle suggesting a potential role for aPKC in the regulation of skeletal muscle function and metabolism during exercise in humans...
  82. ncbi Regulation of the energy sensor AMP-activated protein kinase in the kidney by dietary salt intake and osmolality
    Scott Fraser
    The Austin Research Institute, Victoria, Australia
    Am J Physiol Renal Physiol 288:F578-86. 2005
    ..This study, therefore, demonstrates regulation of AMPK by both a high- and a low-salt intake in vivo and suggests a role for the kinase in the response to changes in osmolality within the kidney...
  83. ncbi AMP-activated protein kinase is not down-regulated in human skeletal muscle of obese females
    Gregory R Steinberg
    St Vincent s Institute, 9 Princes Street, Fitzroy, Victoria, Australia 3065
    J Clin Endocrinol Metab 89:4575-80. 2004
    ....
  84. ncbi Beta-adrenergic stimulation of skeletal muscle HSL can be overridden by AMPK signaling
    Matthew J Watt
    Skeletal Muscle Research Laboratory, School of Medical Sciences, Royal Melbourne Institute of Technology, Bundoora, Victoria, Australia
    FASEB J 18:1445-6. 2004
    ..However, the increased IMTG degradation in LG suggests factors other than HSL activity are important for IMTG degradation...
  85. ncbi Identification of regulatory sites of phosphorylation of the bovine endothelial nitric-oxide synthase at serine 617 and serine 635
    Belinda J Michell
    St. Vincent's Institute of Medical Research, Fitzroy, Victoria 3065, Australia
    J Biol Chem 277:42344-51. 2002
    ..These data show that phosphorylation of both Ser(617) and Ser(635) regulates eNOS activity and contributes to the agonist-stimulated eNOS activation process...
  86. ncbi High-density lipoprotein and apolipoprotein AI increase endothelial NO synthase activity by protein association and multisite phosphorylation
    Brian G Drew
    Baker Heart Research Institute, P.O. Box 6492 St. Kilda Road Central, Melbourne, Victoria 8008, Australia
    Proc Natl Acad Sci U S A 101:6999-7004. 2004
    ..Collectively, the results indicate that HDL and ApoAI increase eNOS activity by multisite phosphorylation changes, involving AMPK activation after protein association between ApoAI and eNOS...
  87. ncbi Regulation of channel gating by AMP-activated protein kinase modulates cystic fibrosis transmembrane conductance regulator activity in lung submucosal cells
    Kenneth R Hallows
    Renal Electrolyte Division, Department of Medicine, University of Pittsburgh School of Medicine, Pennsylvania 15261, USA
    J Biol Chem 278:998-1004. 2003
    ..As a metabolic sensor in cells, AMPK may be important in tuning CFTR activity to cellular energy charge, thereby linking transepithelial transport and the maintenance of cellular ion gradients to cellular metabolism...
  88. ncbi Glutathionyl haemoglobin is not increased in diabetes nor related to glycaemia, complications, dyslipidaemia, inflammation or other measures of oxidative stress
    Peter Hoffmann
    St Vincent s Institute of Medical Research, Fitzroy, Melbourne, Australia
    Diabetes Res Clin Pract 80:e1-3. 2008
    ..Glycated haemoglobin levels were increased in diabetes but glutathionyl haemoglobin (HbSSG) levels did not differ between groups, nor by complications; nor correlate with haemoglobin glycation or vascular risk factors...
  89. ncbi AMPK structure and regulation from three angles
    Bruce E Kemp
    Structure 15:1161-3. 2007
  90. ncbi Adiponectin: starving for attention
    Gregory R Steinberg
    St Vincent s Institute, Department of Medicine, University of Melbourne, 41 Victoria Parade, Fitzroy, Victoria 3065, Australia
    Cell Metab 6:3-4. 2007
    ..A new study from the Kadowaki laboratory (Kubota et al., 2007) shows that the adipocyte-derived hormone adiponectin increases appetite and reduces energy expenditure by stimulating AMPK in the hypothalamus...
  91. ncbi Tumor necrosis factor alpha-induced skeletal muscle insulin resistance involves suppression of AMP-kinase signaling
    Gregory R Steinberg
    St Vincent s Institute and Department of Medicine, University of Melbourne, Fitzroy, Vic, 3065, Australia
    Cell Metab 4:465-74. 2006
    ..Our data demonstrate that AMPK is an important TNFalpha signaling target and is a contributing factor to the suppression of fatty-acid oxidation and the development of lipid-induced insulin resistance in obesity...
  92. ncbi Kinetic properties of nuclear transport conferred by the retinoblastoma (Rb) NLS
    Wei Hu
    Nuclear Signalling Laboratory, Division for Biochemistry and Molecular Biology, John Curtin School of Medical Research, Australian Capital Territory, Canberra, Australia
    J Cell Biochem 95:782-93. 2005
    ....
  93. ncbi Expression and biochemical analysis of the entire HIV-2 gp41 ectodomain: determinants of stability map to N- and C-terminal sequences outside the 6-helix bundle core
    Chan-Sien Lay
    St. Vincent's Institute of Medical Research, and Department of Microbiology and Immunology, University of Melbourne, Victoria, Australia
    FEBS Lett 567:183-8. 2004
    ..A functional interaction between sequences outside the gp41 core may contribute energy to membrane fusion...
  94. ncbi Protein tyrosine phosphatase hPTPN20a is targeted to sites of actin polymerization
    Michelle T Fodero-Tavoletti
    Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Victoria 3800, Australia
    Biochem J 389:343-54. 2005
    ..The present study identifies hPTPN20a as a novel and widely expressed phosphatase with a dynamic subcellular distribution that is targeted to sites of actin polymerization...
  95. ncbi Localization of endothelial nitric-oxide synthase phosphorylated on serine 1179 and nitric oxide in Golgi and plasma membrane defines the existence of two pools of active enzyme
    David Fulton
    Department of Pharmacology and Molecular Cardiobiology Program, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut 06536, USA
    J Biol Chem 277:4277-84. 2002
    ..Thus, activated, phosphorylated eNOS resides in two cellular compartments and both pools are VEGF-regulated to produce NO...