Ursula R Kees

Summary

Affiliation: University of Western Australia
Country: Australia

Publications

  1. ncbi Silencing of TESTIN by dense biallelic promoter methylation is the most common molecular event in childhood acute lymphoblastic leukaemia
    Robert J Weeks
    Cancer Genetics Laboratory, Department of Biochemistry, University of Otago, PO Box 56, Dunedin 9054, New Zealand
    Mol Cancer 9:163. 2010
  2. ncbi Detection of hemizygous deletions in genomic DNA from leukaemia specimens for the diagnosis of patients
    Ursula R Kees
    Telethon Institute for Child Health Research, and Centre for Child Health Research, The University of Western Australia, Perth, Australia
    Leuk Res 29:165-71. 2005
  3. ncbi Gene expression signatures in lymphoid tumours
    Ursula R Kees
    Division of Children s Leukaemia and Cancer Research, Telethon Institute for Child Health Research and Centre for Child Health Research, The University of Western Australia, Perth, Australia
    Immunol Cell Biol 82:154-60. 2004
  4. ncbi Gene expression profiles in a panel of childhood leukemia cell lines mirror critical features of the disease
    Ursula R Kees
    Telethon Institute for Child Health Research, The University of Western Australia, Perth, Australia and National Human Genome Research Institute, NIH, Bethesda, Maryland 20892, USA
    Mol Cancer Ther 2:671-7. 2003
  5. ncbi Identification of novel molecular prognostic markers for paediatric T-cell acute lymphoblastic leukaemia
    Nicholas G Gottardo
    Division of Children s Leukaemia and Cancer Research, Telethon Institute for Child Health Research and Centre for Child Health Research, University of Western Australia, Perth, WA, Australia
    Br J Haematol 137:319-28. 2007
  6. ncbi Prediction of relapse in paediatric pre-B acute lymphoblastic leukaemia using a three-gene risk index
    Katrin Hoffmann
    Division of Children s Leukaemia and Cancer Research, Telethon Institute for Child Health Research, Centre for Child Health Research, Unviersity of Western Australia, Perth, WA, Australia
    Br J Haematol 140:656-64. 2008
  7. ncbi MEIS proteins as partners of the TLX1/HOX11 oncoprotein
    Nadia Milech
    Division of Children s Leukaemia and Cancer Research, Telethon Institute for Child Health Research and Centre for Child Health Research, University of Western Australia, Perth, WA 6008, Australia
    Leuk Res 34:358-63. 2010
  8. ncbi The gene expression signature of relapse in paediatric acute lymphoblastic leukaemia: implications for mechanisms of therapy failure
    Alex H Beesley
    Division of Children's Leukaemia and Cancer Research, Telethon Institute for Child Health Research, University of Western Australia, Perth, WA, Australia
    Br J Haematol 131:447-56. 2005
  9. ncbi TLX1/HOX11 transcription factor inhibits differentiation and promotes a non-haemopoietic phenotype in murine bone marrow cells
    Darcelle N Dixon
    School of Veterinary and Biomedical Sciences, Division of Health Sciences, Murdoch University, Murdoch, Western Australia, Australia
    Br J Haematol 138:54-67. 2007
  10. ncbi Refuelling of vehicles, the use of wood burners and the risk of acute lymphoblastic leukaemia in childhood
    Helen D Bailey
    Telethon Institute for Child Health Research, Centre for Child Health Research, University of Western Australia, Perth, Australia
    Paediatr Perinat Epidemiol 25:528-39. 2011

Research Grants

  1. Prognosis in childhood ALL
    URSULA KEES; Fiscal Year: 2006

Collaborators

Detail Information

Publications46

  1. ncbi Silencing of TESTIN by dense biallelic promoter methylation is the most common molecular event in childhood acute lymphoblastic leukaemia
    Robert J Weeks
    Cancer Genetics Laboratory, Department of Biochemistry, University of Otago, PO Box 56, Dunedin 9054, New Zealand
    Mol Cancer 9:163. 2010
    ..By screening candidate genes, we have detected frequent and dense methylation of the TESTIN (TES) promoter...
  2. ncbi Detection of hemizygous deletions in genomic DNA from leukaemia specimens for the diagnosis of patients
    Ursula R Kees
    Telethon Institute for Child Health Research, and Centre for Child Health Research, The University of Western Australia, Perth, Australia
    Leuk Res 29:165-71. 2005
    ..The qPCR method described is suitable for detection of hemizygous loss in primary patient material and the accuracy of the method was verified by three independent techniques...
  3. ncbi Gene expression signatures in lymphoid tumours
    Ursula R Kees
    Division of Children s Leukaemia and Cancer Research, Telethon Institute for Child Health Research and Centre for Child Health Research, The University of Western Australia, Perth, Australia
    Immunol Cell Biol 82:154-60. 2004
    ..Microarray technology has the potential to lead to more accurate prognostic assessment for patients and is expected to ultimately allow the clinician to select therapies optimally suited to each patient...
  4. ncbi Gene expression profiles in a panel of childhood leukemia cell lines mirror critical features of the disease
    Ursula R Kees
    Telethon Institute for Child Health Research, The University of Western Australia, Perth, Australia and National Human Genome Research Institute, NIH, Bethesda, Maryland 20892, USA
    Mol Cancer Ther 2:671-7. 2003
    ..Importantly, the panel of lines displayed the critical genetic features identified in clinically important acute lymphoblastic leukemia subtypes in childhood leukemia patients...
  5. ncbi Identification of novel molecular prognostic markers for paediatric T-cell acute lymphoblastic leukaemia
    Nicholas G Gottardo
    Division of Children s Leukaemia and Cancer Research, Telethon Institute for Child Health Research and Centre for Child Health Research, University of Western Australia, Perth, WA, Australia
    Br J Haematol 137:319-28. 2007
    ..The independent influence of the 3-gene predictor was confirmed by multivariate analysis. Our study provides proof of principle that genome-wide expression profiling can detect novel molecular prognostic markers in paediatric T-ALL...
  6. ncbi Prediction of relapse in paediatric pre-B acute lymphoblastic leukaemia using a three-gene risk index
    Katrin Hoffmann
    Division of Children s Leukaemia and Cancer Research, Telethon Institute for Child Health Research, Centre for Child Health Research, Unviersity of Western Australia, Perth, WA, Australia
    Br J Haematol 140:656-64. 2008
    ..This is particularly important for the identification of patients destined to relapse despite their current stratification as SR, as more intensive front-line treatment options for these individuals are already available...
  7. ncbi MEIS proteins as partners of the TLX1/HOX11 oncoprotein
    Nadia Milech
    Division of Children s Leukaemia and Cancer Research, Telethon Institute for Child Health Research and Centre for Child Health Research, University of Western Australia, Perth, WA 6008, Australia
    Leuk Res 34:358-63. 2010
    ..Here, we report that TLX1 and MEIS proteins both interact and are co-expressed in T-ALL, and suggest that co-operation between TLX1 and MEIS proteins may have a significant role in T-cell leukemogenesis...
  8. ncbi The gene expression signature of relapse in paediatric acute lymphoblastic leukaemia: implications for mechanisms of therapy failure
    Alex H Beesley
    Division of Children's Leukaemia and Cancer Research, Telethon Institute for Child Health Research, University of Western Australia, Perth, WA, Australia
    Br J Haematol 131:447-56. 2005
    ..Our results provide evidence for a link between drug resistance and the microenvironment that has previously only been considered in the context of solid tumour biology...
  9. ncbi TLX1/HOX11 transcription factor inhibits differentiation and promotes a non-haemopoietic phenotype in murine bone marrow cells
    Darcelle N Dixon
    School of Veterinary and Biomedical Sciences, Division of Health Sciences, Murdoch University, Murdoch, Western Australia, Australia
    Br J Haematol 138:54-67. 2007
    ..This may account for its ability to promote the pre-leukaemic state via perturbation of specific gene expression programs...
  10. ncbi Refuelling of vehicles, the use of wood burners and the risk of acute lymphoblastic leukaemia in childhood
    Helen D Bailey
    Telethon Institute for Child Health Research, Centre for Child Health Research, University of Western Australia, Perth, Australia
    Paediatr Perinat Epidemiol 25:528-39. 2011
    ..There was weak evidence that burning wood in a closed burner to heat the home increased the risk, but there was no dose-response relationship and chance could explain the finding...
  11. ncbi Gene-based outcome prediction in multiple cohorts of pediatric T-cell acute lymphoblastic leukemia: a Children's Oncology Group study
    Amanda L Cleaver
    Division of Children s Leukaemia and Cancer Research, Telethon Institute for Child Health Research, Perth, Australia
    Mol Cancer 9:105. 2010
    ..This study aimed to identify robust gene classifiers that could be used for the accurate prediction of relapse in independent cohorts and across different experimental platforms...
  12. ncbi Gene expression levels assessed by oligonucleotide microarray analysis and quantitative real-time RT-PCR -- how well do they correlate?
    Peter B Dallas
    Division of Children s Leukaemia and Cancer Research, Telethon Institute for Child Health Research and Centre for Child Health Research, The University of Western Australia, Perth, Australia
    BMC Genomics 6:59. 2005
    ..We have assessed the correlation between expression scores obtained for 48 human genes using oligonucleotide microarrays and the expression levels for the same genes measured by quantitative real-time RT-PCR (qRT-PCR)...
  13. ncbi A microarray model system identifies potential new target genes of the proto-oncogene HOX11
    Katrin Hoffmann
    Division of Children s Leukaemia and Cancer Research, Telethon Institute for Child Health Research and Centre for Child Health Research, University of Western Australia, P O Box 855, West Perth WA 6872 Australia
    Genes Chromosomes Cancer 41:309-20. 2004
    ....
  14. ncbi Interactions between acute lymphoblastic leukemia and bone marrow stromal cells influence response to therapy
    Yordanos Tesfai
    Division of Children s Leukaemia and Cancer Research, Telethon Institute for Child Health Research, Centre for Child Health Research, University of Western Australia, Perth, WA, Australia
    Leuk Res 36:299-306. 2012
    ..The data provide novel insight into the pathobiology of ALL and indicate new therapeutic targets for patients with ALL...
  15. ncbi The functional mapping of long-range transcription control elements of the HOX11 proto-oncogene
    Rachael L Brake
    Division of Children s Leukaemia and Cancer Research, Telethon Institute for Child Health Research and Centre for Child Health Research, The University of Western Australia, West Perth, WA 6872, Australia
    Biochem Biophys Res Commun 313:327-35. 2004
    ..This methodology can be applied to any gene cloned into a PAC or BAC vector and could also be useful in identifying appropriately sized deletion mutants for functional testing in transgenic models...
  16. ncbi Exposure to professional pest control treatments and the risk of childhood acute lymphoblastic leukemia
    Helen D Bailey
    Telethon Institute for Child Health Research, Centre for Child Health Research, University of Western Australia, Perth, Western Australia, Australia
    Int J Cancer 129:1678-88. 2011
    ..The analysis of pooled data from international collaborations may provide more certainty regarding these potentially important associations...
  17. ncbi High expression of connective tissue growth factor in pre-B acute lymphoblastic leukaemia
    Joanne M Boag
    Division of Children s Leukaemia and Cancer Research, Telethon Institute for Child Health Research, and Centre for Child Health Research, The University of Western Australia, West Perth, WA, Australia
    Br J Haematol 138:740-8. 2007
    ..Protein studies using Western blot analysis demonstrated the presence of CTGF in ALL cell-conditioned media. These findings indicate that CTGF is secreted by pre-B ALL cells and may play a role in the pathophysiology of this disease...
  18. ncbi Translating microarray data for diagnostic testing in childhood leukaemia
    Katrin Hoffmann
    Division of Children s Leukaemia and Cancer Research, Telethon Institute for Child Health Research and Centre for Child Health Research, The University of Western Australia, Perth, Australia
    BMC Cancer 6:229. 2006
    ..As a step towards clinical application of these findings, we have systematically analyzed a published ALL microarray data set using Robust Multi-array Analysis (RMA) and Random Forest (RF)...
  19. ncbi Influence of wild-type MLL on glucocorticoid sensitivity and response to DNA-damage in pediatric acute lymphoblastic leukemia
    Alex H Beesley
    Division of Children s Leukaemia and Cancer Research, Telethon Institute for Child Health Research, University of Western Australia Centre for Child Health Research, Perth, Australia
    Mol Cancer 9:284. 2010
    ..We have recently observed that GC resistance in T-ALL cell lines is associated with a proliferative metabolism and reduced expression of MLL. In this study we have further explored the relationship between MLL status and GC sensitivity...
  20. ncbi In vitro cytotoxicity of nelarabine, clofarabine and flavopiridol in paediatric acute lymphoblastic leukaemia
    Alex H Beesley
    Division of Children s Leukaemia and Cancer Research, Telethon Institute for Child Health Research, and Centre for Child Health Research, University of Western Australia, Perth, WA, Australia
    Br J Haematol 137:109-16. 2007
    ..FP should be widely effective in ALL if sufficient plasma levels can be achieved clinically...
  21. ncbi Specific alternative HOX11 transcripts are expressed in paediatric neural tumours and T-cell acute lymphoblastic leukaemia
    Paul M Watt
    Division of Children's Leukaemia and Cancer Research, Telethon Institute for Child Health Research and Centre for Child Health Research, The University of Western Australia, P O Box 855, Western Australia 6872, West Perth, Australia
    Gene 323:89-99. 2003
    ..These observations, combined with sequence data from several EST clones derived from medulloblastoma cDNA libraries, support a new hypothesis that HOX11 may also function as a neural oncogene or brain tumour marker...
  22. ncbi Classic and desmoplastic medulloblastoma: complete case reports and characterizations of two new cell lines
    David J Holthouse
    Telethon Institute for Child Health Research and Centre for Child Health Research, University of Western Australia, Perth, Australia
    Neuropathology 29:398-409. 2009
    ..These cell lines represent an additional resource for the analysis of diverse aspects of MB biology...
  23. ncbi Transcriptional regulation of FHL1 by TLX1/HOX11 is dosage, cell-type and promoter context-dependent
    Kim L Rice
    School of Veterinary and Biomedical Sciences, Division of Health Sciences, Murdoch University, Perth, WA 6150, Australia
    Biochem Biophys Res Commun 367:707-13. 2008
    ..Together, these results characterize TLX1 as a dual function regulator whose activity in respect to FHL1 is critically dependent upon its cellular concentration, as well as cell type and promoter context...
  24. ncbi Gene expression levels in small specimens from patients detected using oligonucleotide arrays
    Katrin Hoffmann
    Division of Children s Leukaemia and Cancer Research, Telethon Institute for Child Health Research, P O Box 855, West Perth WA 6872, Australia
    Mol Biotechnol 29:31-8. 2005
    ..Most importantly, there was no statistically significant difference when the concordance percentages for low abundance genes were compared, demonstrating that as little as 2 microg of total RNA is sufficient to perform GeneChip analysis...
  25. ncbi Receptor mutation is not a common mechanism of naturally occurring glucocorticoid resistance in leukaemia cell lines
    Alex H Beesley
    Division of Children s Leukaemia and Cancer Research, Telethon Institute for Child Health Research, University of Western Australia Centre for Child Health Research, Perth, Australia
    Leuk Res 33:321-5. 2009
    ..Resistance could not be attributed to mutations in GR or variations in levels of its expression. We conclude that this panel of cell lines provides a suitable in vitro model since it reflects GC resistance in primary ALL...
  26. ncbi Deletion of one copy of the p16INK4A tumor suppressor gene is implicated as a predisposing factor in pediatric leukemia
    Tina L Carter
    Division of Children s Leukaemia and Cancer Research, Telethon Institute for Child Health Research and Centre for Child Health Research, The University of Western Australia, Perth, Australia
    Biochem Biophys Res Commun 318:852-5. 2004
    ..This is particularly true for the emerging class of tumor suppressor genes where deletion of one allele is sufficient to confer cancer susceptibility or poor prognosis with standard treatment...
  27. ncbi FusionFinder: a software tool to identify expressed gene fusion candidates from RNA-Seq data
    Richard W Francis
    Division of Bioinformatics and Biostatistics, Telethon Institute for Child Health Research, Centre for Child Health Research, The University of Western Australia, Perth, Australia
    PLoS ONE 7:e39987. 2012
    ..FusionFinder is made freely available for non-commercial use and can be downloaded from the project website (http://bioinformatics.childhealthresearch.org.au/software/fusionfinder/)...
  28. ncbi Aberrant over-expression of a forkhead family member, FOXO1A, in a brain tumor cell line
    Peter B Dallas
    Division of Children s Leukemia and Cancer Research, Telethon Institute for Child Health Research and Centre for Child Health Research, University of Western Australia, Perth, Australia
    BMC Cancer 7:67. 2007
    ..We have addressed the mechanisms underlying the high level of expression of the FOXO1A gene in a cell line, PER-453, derived from a primitive neuroectodermal tumor of the central nervous system (CNS-PNET)...
  29. ncbi Effective adenovirus-mediated gene transfer into neural stem cells derived from human embryonic stem cells
    Cornelia M Bertram
    Brain Tumour Research Program, Telethon Institute for Child Health Research and Centre for Child Health Research, University of Western Australia, Perth, Australia
    Stem Cells Dev 19:569-78. 2010
    ..This model system provides a practical new approach to study human NSC function in the context of neurodegenerative and neoplastic disorders...
  30. ncbi Exposure to diagnostic radiological procedures and the risk of childhood acute lymphoblastic leukemia
    Helen D Bailey
    Telethon Institute for Child Health Research, Centre for Child Health Research, The University of Western Australia, Perth, WA, Australia
    Cancer Epidemiol Biomarkers Prev 19:2897-909. 2010
    ..The aim of this analysis is to investigate whether diagnostic X-rays of the mother before birth, of the father before conception, or of the child increased the risk of childhood ALL...
  31. ncbi Validation of a mouse xenograft model system for gene expression analysis of human acute lymphoblastic leukaemia
    Amy L Samuels
    Division of Children s Leukaemia and Cancer Research, Telethon Institute for Child Health Research, Perth, Western Australia
    BMC Genomics 11:256. 2010
    ..However, for this model to be effective for studying engraftment and therapy responses at the whole genome level, careful molecular characterisation is essential...
  32. ncbi Enforced expression of HOX11 is associated with an immature phenotype in J2E erythroid cells
    Wayne K Greene
    Division of Children s Leukaemia and Cancer Research, TVW Telethon Institute for Child Health Research and Centre for Child Health Research, University of Western Australia, West Perth, Australia
    Br J Haematol 118:909-17. 2002
    ..This supports the notion that disrupted haematopoietic cell differentiation is responsible for pre-leukaemic immortalization by the HOX11 oncoprotein...
  33. ncbi Genomic deletions in cell lines derived from primitive neuroectodermal tumors of the central nervous system
    Peter B Dallas
    Division of Children s Leukemia and Cancer Research, Telethon Institute for Child Health Research and Center for Child Health Research, The University of Western Australia, P O Box 855, West Perth, WA 6872, Australia
    Cancer Genet Cytogenet 159:105-13. 2005
    ..The identification of specific genes and signaling pathways that are compromised in CNS-PNET cells is crucial for development of more efficacious and less invasive treatments, as are urgently needed...
  34. ncbi Is there a folate-related gene-environment interaction in the etiology of childhood acute lymphoblastic leukemia?
    Elizabeth Milne
    Telethon Institute for Child Health Research, Centre for Child Health Research, The University of Western Australia, WA, Australia
    Int J Cancer 119:229-32. 2006
    ..Further research into the interaction of folate intake and genotype in causing ALL and other cancers is needed. We are specifically studying it in an Australian national case-control study of genetic and environmental causes of ALL...
  35. ncbi The diphtheria toxin/urokinase fusion protein (DTAT) is selectively toxic to CD87 expressing leukemic cells
    Jason G Ramage
    Department of Cancer Biology, Wake Forest University School of Medicine, Medical Center Boulevard, 27157, Winston Salem, NC, USA
    Leuk Res 27:79-84. 2003
    ....
  36. ncbi Regions syntenic to human 17q are gained in mouse and rat neuroblastoma
    Maria Łastowska
    Institute of Human Genetics, International Centre for Life, University of Newcastle upon Tyne, Newcastle upon Tyne, United Kingdom
    Genes Chromosomes Cancer 40:158-63. 2004
    ..2 Mb from the previously defined region of 17q gain in humans as a likely location of the candidate gene or genes, and strongly suggests that the molecular etiology of neuroblastoma is similar in all three species...
  37. ncbi Expression and purification of the human homeodomain oncoprotein HOX11
    Mansour Heidari
    Division of Veterinary and Biomedical Sciences, Western Australian Biomedical Research Institute, Murdoch University, Murdoch, WA 6150, Australia
    Protein Expr Purif 25:313-8. 2002
    ..The biological activity of the recombinant protein was verified by the specific binding of GST-HOX11, but not GST-HOX11 Delta H3, to DNA containing consensus HOX11 recognition sites...
  38. ncbi Cell lines from MYCN transgenic murine tumours reflect the molecular and biological characteristics of human neuroblastoma
    Andy J Cheng
    Children s Cancer Institute Australia for Medical Research, P O Box 81, Randwick, 2031 Sydney, Australia
    Eur J Cancer 43:1467-75. 2007
    ..These isogenic lines together with the transgenic mice thus represent valuable models for investigating the biological characteristics of aggressive neuroblastoma...
  39. ncbi Divergent mechanisms of glucocorticoid resistance in experimental models of pediatric acute lymphoblastic leukemia
    Petra S Bachmann
    Children s Cancer Institute Australia for Medical Research, The University of New South Wales, Sydney, Australia
    Cancer Res 67:4482-90. 2007
    ....
  40. ncbi The role of MYCN in the failure of MYCN amplified neuroblastoma cell lines to G1 arrest after DNA damage
    Emma Bell
    Northern Institute for Cancer Research, University of Newcastle upon Tyne, Newcastle upon Tyne, UK
    Cell Cycle 5:2639-47. 2006
    ..This study is the first to determine that MNA correlates with a failure to G1 arrest and attenuated p21(WAF1) induction; however MYCN expression alone is not causally responsible...
  41. ncbi Increased frequency of aberrations in the p53/MDM2/p14(ARF) pathway in neuroblastoma cell lines established at relapse
    Jane Carr
    Northern Institute for Cancer Research, University of Newcastle upon Tyne, United Kingdom
    Cancer Res 66:2138-45. 2006
    ..If these data are confirmed in neuroblastoma tumors, this suggests that p53-independent therapy and reactivation of inactive p53 approaches would be useful in the management of relapsed neuroblastoma...
  42. ncbi A promoter with bidirectional activity is located between TLX1/HOX11 and a divergently transcribed novel human gene
    Wayne K Greene
    School of Veterinary and Biomedical Sciences, Division of Health Sciences, Murdoch University, Perth WA 6150, Australia
    Gene 391:223-32. 2007
    ..Our finding that TDI is transcriptionally co-activated in leukemic cells that aberrantly express TLX1, additionally suggests that it may have the potential to act as a co-operating oncogene in leukemogenesis...
  43. ncbi Overexpression of stem cell associated ALDH1A1, a target of the leukemogenic transcription factor TLX1/HOX11, inhibits lymphopoiesis and promotes myelopoiesis in murine hematopoietic progenitors
    Kim L Rice
    School of Veterinary and Biomedical Sciences, Division of Health Sciences, Murdoch University, South Street, Murdoch, Perth WA 6150, Australia
    Leuk Res 32:873-83. 2008
    ....
  44. ncbi Relapse in children with acute lymphoblastic leukemia involving selection of a preexisting drug-resistant subclone
    Seoyeon Choi
    Children s Cancer Institute Australia for Medical Research, Sydney, Australia
    Blood 110:632-9. 2007
    ..Relapse prediction may be improved with strategies to detect minor potentially resistant subclones early during treatment, hence allowing intensification of therapy...
  45. ncbi Mechanism of relapse in pediatric acute lymphoblastic leukemia
    Michelle J Henderson
    Children s Cancer Institute Australiafor Medical Research, Experimental Therapeutics Program, PO Box 81 HighSt, Randwick, Sydney, NSW 2031 Australia
    Cell Cycle 7:1315-20. 2008
    ....
  46. ncbi A xenograft model of infant leukaemia reveals a complex MLL translocation
    Michelle J Henderson
    Br J Haematol 140:716-9. 2008

Research Grants1

  1. Prognosis in childhood ALL
    URSULA KEES; Fiscal Year: 2006
    ....