Bernd H Kalinna

Summary

Affiliation: The University of Melbourne
Location: Melbourne, Australia
Grants:
Somatic gene trapping in Schistosoma mansoni, 2011, National Health & Medical Research Council of Australia

Targeting Bcl-2 pathways in parasites, 2011, National Health & Medical Research Council of Australia

Publications

  1. pmc Advances in mRNA silencing and transgene expression: a gateway to functional genomics in schistosomes
    Elissaveta Tchoubrieva
    Centre for Animal Biotechnology, Faculty of Veterinary Science, The University of Melbourne, Parkville, 3010 Vic, Australia
    Biotechnol Genet Eng Rev 26:261-80. 2010
  2. pmc Discovery and molecular characterization of a Bcl-2-regulated cell death pathway in schistosomes
    Erinna F Lee
    The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia
    Proc Natl Acad Sci U S A 108:6999-7003. 2011
  3. ncbi request reprint Genomic organization of the Schistosoma mansoni aspartic protease gene, a platyhelminth orthologue of mammalian lysosomal cathepsin D
    Maria E Morales
    Department of Tropical Medicine, School of Public Health and Tropical Medicine, Tulane University Health Sciences Center, New Orleans, LA 70112, USA
    Gene 338:99-109. 2004
  4. ncbi request reprint Structural and evolutionary analysis of the transcribed sequence of Boudicca, a Schistosoma mansoni retrotransposon
    Claudia S Copeland
    Department of Molecular Parasitology, Institute for Biology, Humboldt University Berlin, Berlin, Germany
    Gene 329:103-14. 2004
  5. ncbi request reprint Schistosoma mansoni miracidia transformed by particle bombardment infect Biomphalaria glabrata snails and develop into transgenic sporocysts
    Oliver Heyers
    Department of Molecular Parasitology, Institute for Biology, Humboldt University Berlin, 10115 Berlin, Germany
    Exp Parasitol 105:174-8. 2003
  6. ncbi request reprint Trans-species transfer of Wolbachia: microinjection of Wolbachia from litomosoides sigmodontis into Acanthocheilonema viteae
    N Hartmann
    Department of Molecular Parasitology, Institute of Biology, Humboldt University, Berlin, Germany
    Parasitology 126:503-11. 2003
  7. pmc Boudicca, a retrovirus-like long terminal repeat retrotransposon from the genome of the human blood fluke Schistosoma mansoni
    Claudia S Copeland
    Department of Tropical Medicine, School of Public Health and Tropical Medicine, Tulane University Health Sciences Center, New Orleans, Louisiana 70112, USA
    J Virol 77:6153-66. 2003
  8. ncbi request reprint [Expression and purification of recombinant Schistosoma japonicum paramyosin]
    J C Zhou
    Department of Parasitology, Hunan Medical University, Changsha 410078
    Hunan Yi Ke Da Xue Xue Bao 25:106-8. 2000
  9. ncbi request reprint Recombinant paramyosin (rec-Sj-97) tested for immunogenicity and vaccine efficacy against Schistosoma japonicum in mice and water buffaloes
    D P McManus
    Molecular Parasitology Laboratory, Australian Centre for International and Tropical Health and Nutrition, The Queensland Institute of Medical Research, 300 Herston Road, Queensland 4029, Brisbane, Australia
    Vaccine 20:870-8. 2001
  10. ncbi request reprint Immunisation with Salmonella typhimurium-delivered glyceraldehyde-3-phosphate dehydrogenase protects mice against challenge infection with Echinococcus multilocularis eggs
    V Müller-Schollenberger
    Division of Parasitology, Department of Zoology, University of Hohenheim, 70599 Stuttgart, Germany
    Int J Parasitol 31:1441-9. 2001

Collaborators

Detail Information

Publications42

  1. pmc Advances in mRNA silencing and transgene expression: a gateway to functional genomics in schistosomes
    Elissaveta Tchoubrieva
    Centre for Animal Biotechnology, Faculty of Veterinary Science, The University of Melbourne, Parkville, 3010 Vic, Australia
    Biotechnol Genet Eng Rev 26:261-80. 2010
    ..Until recently, gene manipulation approaches had not been seriously addressed. This situation is now changing and rapid advances have been made in gene silencing and transgenesis of schistosomes...
  2. pmc Discovery and molecular characterization of a Bcl-2-regulated cell death pathway in schistosomes
    Erinna F Lee
    The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia
    Proc Natl Acad Sci U S A 108:6999-7003. 2011
    ....
  3. ncbi request reprint Genomic organization of the Schistosoma mansoni aspartic protease gene, a platyhelminth orthologue of mammalian lysosomal cathepsin D
    Maria E Morales
    Department of Tropical Medicine, School of Public Health and Tropical Medicine, Tulane University Health Sciences Center, New Orleans, LA 70112, USA
    Gene 338:99-109. 2004
    ..Taken together, these features indicated that schistosome cathepsin D is a platyhelminth orthologue of mammalian lysosomal cathepsin D...
  4. ncbi request reprint Structural and evolutionary analysis of the transcribed sequence of Boudicca, a Schistosoma mansoni retrotransposon
    Claudia S Copeland
    Department of Molecular Parasitology, Institute for Biology, Humboldt University Berlin, Berlin, Germany
    Gene 329:103-14. 2004
    ..In addition, phylogenetic analysis targeting discrete domains of Boudicca revealed a generalized radiation in sequences among the multiple copies of Boudicca resident in the schistosome genome...
  5. ncbi request reprint Schistosoma mansoni miracidia transformed by particle bombardment infect Biomphalaria glabrata snails and develop into transgenic sporocysts
    Oliver Heyers
    Department of Molecular Parasitology, Institute for Biology, Humboldt University Berlin, 10115 Berlin, Germany
    Exp Parasitol 105:174-8. 2003
    ..Our findings indicated that it is feasible to return transgenic miracidia to the life cycle, a crucial step for the establishment of a transgenesis system for schistosomes...
  6. ncbi request reprint Trans-species transfer of Wolbachia: microinjection of Wolbachia from litomosoides sigmodontis into Acanthocheilonema viteae
    N Hartmann
    Department of Molecular Parasitology, Institute of Biology, Humboldt University, Berlin, Germany
    Parasitology 126:503-11. 2003
    ..The DNA of the microinjected worms recovered from jirds 8 weeks after implantation contained Wolbachia DNA as shown by PCR, suggesting that Wolbachia of L. sigmodontis can be horizontally transmitted to A. viteae...
  7. pmc Boudicca, a retrovirus-like long terminal repeat retrotransposon from the genome of the human blood fluke Schistosoma mansoni
    Claudia S Copeland
    Department of Tropical Medicine, School of Public Health and Tropical Medicine, Tulane University Health Sciences Center, New Orleans, Louisiana 70112, USA
    J Virol 77:6153-66. 2003
    ..By reverse transcription-PCR, mRNA transcripts were detected in the sporocyst, cercaria, and adult developmental stages of S. mansoni, indicating that Boudicca is actively transcribed in this trematode...
  8. ncbi request reprint [Expression and purification of recombinant Schistosoma japonicum paramyosin]
    J C Zhou
    Department of Parasitology, Hunan Medical University, Changsha 410078
    Hunan Yi Ke Da Xue Xue Bao 25:106-8. 2000
    ..To eliminate imidazole and residue of E. coli, the elution was further purified by ion-exchange chromatography. The purified protein will be used in water buffaloes in the study on protective immunity against Schistosoma japonicum...
  9. ncbi request reprint Recombinant paramyosin (rec-Sj-97) tested for immunogenicity and vaccine efficacy against Schistosoma japonicum in mice and water buffaloes
    D P McManus
    Molecular Parasitology Laboratory, Australian Centre for International and Tropical Health and Nutrition, The Queensland Institute of Medical Research, 300 Herston Road, Queensland 4029, Brisbane, Australia
    Vaccine 20:870-8. 2001
    ....
  10. ncbi request reprint Immunisation with Salmonella typhimurium-delivered glyceraldehyde-3-phosphate dehydrogenase protects mice against challenge infection with Echinococcus multilocularis eggs
    V Müller-Schollenberger
    Division of Parasitology, Department of Zoology, University of Hohenheim, 70599 Stuttgart, Germany
    Int J Parasitol 31:1441-9. 2001
    ..These data emphasise that antigen delivery systems play a critical role in vaccination and the induction of protective immunity against helminth parasites...
  11. ncbi request reprint Organization and functional analysis of the Schistosoma mansoni cathepsin D-like aspartic protease gene promoter
    Alexandra Schulmeister
    Department of Molecular Parasitology, Institute of Biology, Humboldt University Berlin, Philippstrasse 13, 10115 Berlin, Germany
    Biochim Biophys Acta 1727:27-34. 2005
    ....
  12. pmc The Sinbad retrotransposon from the genome of the human blood fluke, Schistosoma mansoni, and the distribution of related Pao-like elements
    Claudia S Copeland
    Department of Tropical Medicine, Tulane University Health Sciences Center, New Orleans, USA
    BMC Evol Biol 5:20. 2005
    ..It is becoming apparent that numerous LTR retrotransposons and other mobile genetic elements have colonized the genome of the human blood fluke, Schistosoma mansoni...
  13. pmc Studies on Acanthocheilonema viteae cystatin: genomic organization, promoter studies and expression in Caenorhabditis elegans
    Smitha Pillai
    Department of Molecular Parasitology, Institute of Biology, Humboldt University Berlin, 10115 Berlin, Germany
    Filaria J 4:9. 2005
    ..Additionally, A. viteae cystatin was expressed in C. elegans to explore its potential as an expression system for filarial genes...
  14. ncbi request reprint A special issue on immunology and cell biology of protozoa
    Bernd H Kalinna
    Exp Parasitol 126:281-2. 2010
  15. doi request reprint Vector-based RNA interference of cathepsin B1 in Schistosoma mansoni
    Elissaveta B Tchoubrieva
    Centre for Animal Biotechnology, Faculty of Veterinary Science, The University of Melbourne, Parkville, Vic, 3052, Australia
    Cell Mol Life Sci 67:3739-48. 2010
    ..This is the first report using this technology in any helminth parasite. It will facilitate the evaluation of potential drug targets and biochemical pathways for novel interventions in schistosomes...
  16. doi request reprint Characterisation of recombinant immunoreactive antigens of the scab mite Sarcoptes scabiei
    C Kuhn
    Department of Molecular Parasitology, Institute of Biology, Humboldt University Berlin, Berlin, Germany
    Vet Parasitol 153:329-37. 2008
    ..Three of the proteins contain repetitive sequences which suggests that they might be involved in immune evasion. The application of these antigens in serodiagnosis and the suitability for diagnosis is discussed...
  17. ncbi request reprint piggyBac transposon mediated transgenesis of the human blood fluke, Schistosoma mansoni
    Maria E Morales
    Department of Tropical Medicine, Tulane University, Health Sciences Center, 1430 Tulane Ave, New Orleans, LA 70112, USA
    FASEB J 21:3479-89. 2007
    ..This is the first report of chromosomal integration of a transgene and somatic transgenesis of this important human pathogen, in this instance accomplished by mobilization of the piggyBac transposon...
  18. ncbi request reprint Manipulating the manipulators: advances in parasitic helminth transgenesis and RNAi
    Bernd H Kalinna
    Centre for Animal Biotechnology, Faculty of Veterinary Science, The University of Melbourne, Parkville, 3010 Vic, Australia
    Trends Parasitol 23:197-204. 2007
    ..This situation is now changing in response to the availability of genome sequences and other advances. In this article, we review advances in the transgenesis and gene silencing of parasitic worms...
  19. ncbi request reprint Transduction of Schistosoma mansoni by vesicular stomatitis virus glycoprotein-pseudotyped Moloney murine leukemia retrovirus
    Kristine J Kines
    Department of Tropical Medicine and Center for Infectious Diseases, Tulane University, Health Sciences Center, New Orleans, LA 70112, USA
    Exp Parasitol 112:209-20. 2006
    ..Together these results indicated productive transduction by VSVG pseudotyped MMLV of cultured schistosomes, and suggest a tractable route forward towards heritable schistosome transgenesis...
  20. ncbi request reprint A nematode allergen elicits protection against challenge infection under specific conditions
    Susanne Hartmann
    Department of Molecular Parasitology Institute of Biology, Humboldt University Berlin, Philippstr 13, D 10115 Berlin, Germany
    Vaccine 24:3581-90. 2006
    ..Our data suggest that vaccination with tropomyosin under Th1 conditions, which are atypical for nematode infections, induces protection via an antibody independent effector mechanism...
  21. ncbi request reprint Proteolysis of human hemoglobin by schistosome cathepsin D
    P J Brindley
    Molecular Parasitology Unit, Queensland Institute of Medical Research, University of Queensland, Brisbane, Australia
    Mol Biochem Parasitol 112:103-12. 2001
    ....
  22. pmc Differential antigen-stimulated proliferation of human mononuclear cells by recombinant Schistosoma japonicum antigens in a Chinese population
    G J Waine
    Molecular Parasitology Unit, The Queensland Institute of Medical Research, PO Royal Brisbane Hospital, Australia
    Clin Exp Immunol 112:69-73. 1998
    ..The identification of differential cellular proliferative responses to specific schistosome antigens within an infected human population may have important practical implications for vaccine development against schistosomiasis japonica...
  23. ncbi request reprint Codon usage in Echinococcus
    B H Kalinna
    Molecular Parasitology Unit, Queensland Institute of Medical Research, Bancroft Centre, Brisbane, Australia
    Exp Parasitol 79:72-6. 1994
  24. ncbi request reprint Gene cloning, overproduction and purification of a functionally active cytoplasmic fatty acid-binding protein (Sj-FABPC) from the human blood fluke Schistosoma japonicum
    M M Becker
    Molecular Parasitology Unit, Queensland Institute of Medical Research, Brisbane, Australia
    Gene 148:321-5. 1994
    ..The purified re-Sj-FABPC was also shown to bind to palmitic acid with high affinity. The functional expression of Sj-FABPC will facilitate studies on re-Sj-FABPC to assess its potential as a drug and/or vaccine candidate...
  25. ncbi request reprint Gene cloning and complete nucleotide sequence of philippine Schistosoma japonicum paramyosin
    M M Becker
    Molecular Parasitology Unit, Queensland Institute of Medical Research, Bancroft Centre, Brisbane, Australia
    Acta Trop 59:143-7. 1995
    ..1991; Dahmen et al., 1993; Landa et al., 1993; Mühlschlegel et al., 1993, Nara et al., 1994). The cloning and nucleotide sequence of S. Japonicum paramyosin is described...
  26. ncbi request reprint Schistosoma japonicum: heterogeneity in paramyosin genes
    C W Hooker
    Molecular Parasitology Unit, Queensland Institute of Medical Research, Brisbane, Australia
    Acta Trop 59:131-41. 1995
    ..japonicum...
  27. ncbi request reprint Cloning and characterization of the Schistosoma japonicum aspartic proteinase involved in hemoglobin degradation
    M M Becker
    Molecular Parasitology Unit, Queensland Institute of Medical Research, Royal Brisbane Hospital, Australia
    J Biol Chem 270:24496-501. 1995
    ..The schistosome aspartic proteinase may play a pivotal role in the catabolism of hemoglobin obtained from host erythrocytes...
  28. ncbi request reprint Comparison of the patterns of codon usage and bias between Brugia, Echinococcus, Onchocerca and Schistosoma species
    J Ellis
    Faculty of Science, University of Technology Sydney, New South Wales, Australia
    Parasitol Res 81:388-93. 1995
    ..The codon usage of Echinococcus was distinct from that of the other taxa. Furthermore, the pattern of codon usage detected by the average codon usage summed across all genes for each taxon was not shown by all genes from that taxon...
  29. ncbi request reprint Taenia crassiceps metacestode vesicular fluid antigens shared with the Taenia solium larval stage and reactive with serum antibodies from patients with neurocysticercosis
    J Kunz
    Fachbereich Biologie, Philipps Universitat, Marburg
    Zentralbl Bakteriol 271:510-20. 1989
    ..Altogether ten 125I-labelled proteins (Mr range from about 20,000 to 200,000) were recognized by neurocysticerosis antibodies. Four proteins (Mr about 22,000, 25,000, 32,000 and 45,000) were detected by all sera...
  30. ncbi request reprint Immunoelectrophoretic analyses of antigens shared by the vesicular fluid and cyst wall of Taenia crassiceps and Taenia saginata metacestodes
    B Kalinna
    Fachbereich Biologie, , Marburg, Federal Republic of Germany
    Parasitol Res 75:568-74. 1989
    ..saginata metacestodes from heavily and moderately infected calves (n = 6) by ELISA, VF and CWE antigens of both Taenia species were found to be potent reagents; TsVF was the most sensitive antigen...
  31. ncbi request reprint Purification of a recombinant Schistosoma japonicum antigen homologous to the 22-kDa membrane-associated antigen of S. mansoni, a putative vaccine candidate against schistosomiasis
    G J Waine
    Molecular Parasitology Unit, Queensland Institute of Medical Research, Brisbane, Australia
    Gene 142:259-63. 1994
    ..The recombinant protein was purified under non-denaturing conditions using nickel-chelate affinity chromatography...
  32. ncbi request reprint Cloning and functional expression of a Schistosoma japonicum cDNA homologous to the enolase gene family
    G J Waine
    Molecular Parasitology Unit, Queensland Institute of Medical Research, Bancroft Centre, Brisbane, Australia
    Biochem Biophys Res Commun 195:1211-7. 1993
    ..After subcloning and expression, the recombinant protein was purified by affinity chromatography under non-denaturing conditions and shown to exhibit functional enolase enzymatic activity...
  33. pmc Cloning, molecular characterization, and functional activity of Schistosoma japonicum glyceraldehyde-3-phosphate dehydrogenase, a putative vaccine candidate against schistosomiasis japonica
    G J Waine
    Molecular Parasitology Unit, Queensland Institute of Medical Research, Bancroft Centre, Brisbane, Australia
    Infect Immun 61:4716-23. 1993
    ..After subcloning in the QIAexpress vector pQE-10 and subsequent expression, the recombinant protein was purified under nondenaturing conditions and shown to exhibit functional GAPDH enzymatic activity...
  34. ncbi request reprint A vaccine against the Asian schistosome, Schistosoma japonicum: an update on paramyosin as a target of protective immunity
    B H Kalinna
    Molecular Parasitology Unit, Australian Centre for International and Tropical Health and Nutrition, Queensland Institute of Medical Research, Bancroft Centre, Royal Brisbane Hospital, Australia
    Int J Parasitol 27:1213-9. 1997
    ..Here we discuss the literature of the past decade and report on different recombinant paramyosin constructs we are using in our laboratory to develop a vaccine against the Asian schistosoma, Schistosoma japonicum...
  35. ncbi request reprint DNA vaccines for parasitic infections
    B H Kalinna
    Molecular Parasitology Unit, Australian Centre for International and Tropical Health and Nutrition, Queensland Institute of Medical Research, Bancroft Centre, Brisbane, Australia
    Immunol Cell Biol 75:370-5. 1997
    ..In the future, nucleic acid vaccines will be a useful tool to help control these and other parasitic infections...
  36. ncbi request reprint Proteolytic degradation of host hemoglobin by schistosomes
    P J Brindley
    Molecular Parasitology Unit, Queensland Institute of Medical Research, Royal Brisbane Hospital, Australia
    Mol Biochem Parasitol 89:1-9. 1997
    ..More specifically, the review concentrates on five proteolytic enzymes, and their associated genes, which have been ascribed key roles in the pathway of hemoglobin degradation...
  37. ncbi request reprint Cloning and expression of a cDNA encoding a nonintegrin laminin-binding protein from Echinococcus granulosus with localization of the laminin-binding domain
    L Zhang
    Molecular Parasitology Unit, Australian Centre for International and Tropical Health and Nutrition, Bancroft Centre, Brisbane QLD, Australia
    Mol Biochem Parasitol 87:183-92. 1997
    ..As such, the egmo3 protein may be multifunctional in E. granulosus, acting as a laminin-binding molecule but also playing a role in cell division and growth...
  38. ncbi request reprint DNA-based vaccination using Schistosoma japonicum (Asian blood-fluke) genes
    G J Waine
    Molecular Parasitology Unit, Australian Centre for International and Tropical Health and Nutrition, Queensland Institute of Medical Research, Royal Brisbane Hospital, Herston, Australia
    Vaccine 15:846-8. 1997
    ..In contrast, none of the other schistosome cDNAs tested were able to induce detectable antibody responses. The anti-paramyosin antibodies did not protect mice challenged with cercariae of S. japonicum...
  39. ncbi request reprint Engineering and expression of a full length cDNA encoding Schistosoma japonicum paramyosin. Purification of the recombinant protein and its recognition by infected patient sera
    B H Kalinna
    Molecular Parasitology Unit, Queensland Institute of Medical Research, Bancroft Centre, Royal Brisbane Hospital, Australia
    Acta Trop 65:111-5. 1997
    ..The engineering of the full-length cDNA encoding Schistosoma japonicum paramyosin, its bacterial expression and purification will facilitate future studies aimed at determining its efficacy as an anti-schistosomiasis vaccine...
  40. ncbi request reprint Cloning and characterization of a ribosomal P protein from Taenia solium, the aetiological agent of human cysticercosis
    B H Kalinna
    Molecular Parasitology Unit, Queensland Institute of Medical Research, Bancroft Centre, Brisbane, Australia
    Biochem Biophys Res Commun 219:231-7. 1996
    ..5 kDa arpP2 plus an additional 2 kDa for the N-terminal fusion peptide incorporating the six histidine residues required for purification...
  41. ncbi request reprint An IgG (Fc gamma)-binding protein of Taenia crassiceps (Cestoda) exhibits sequence homology and antigenic similarity with schistosome paramyosin
    B Kalinna
    Molecular Helminthology Laboratory, Queensland Institute of Medical Research, Brisbane, Australia
    Parasitology 106:289-96. 1993
    ..crassiceps molecule suggests that this class of proteins may be multifunctional, fulfilling not only a structural role but also as components interacting with the host immune system to increase parasite survival...
  42. ncbi request reprint Purification of an antigen from the Taenia crassiceps metacestode vesicular fluid highly sensitive in detecting IgG-antibodies (ELISA) in calves with experimental cysticercosis
    B Kalinna
    Fachbereich Biologie, Philipps Universitat, Marburg Lahn
    Zentralbl Veterinarmed B 37:213-21. 1990
    ..As shown by immunodiffusion and ELISA the antigen is also common to the T. saginata adult stage and obviously to other taeniid metacestodes where it is accumulated in the VF or hydatid fluid...