Research Topics
Genomes and GenesSpecies | S D JohnstonSummaryAffiliation: University of Queensland Country: Australia Publications
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Publications
Testosterone secretion, testicular histology and the cryopreservation of cauda epididymidal spermatozoa in the common ringtail possum (Pseudocheirus peregrinus)D J Phillips
School of Animal Studies, The University of Queensland, Gatton, QLD 4343, Australia
Reprod Fertil Dev 20:391-401. 2008..Similar to other marsupial sperm, common ringtail possum cauda epididymidal spermatozoa required high levels of glycerol (10-16%) in order to maintain post-thaw viability...
The multiple sex chromosomes of platypus and echidna are not completely identical and several share homology with the avian ZWillem Rens
Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 OES, UK
Genome Biol 8:R243. 2007..In order to investigate monotreme sex chromosome evolution, we performed a comparative study of platypus and echidna by chromosome painting and comparative gene mapping...
An investigation into the similarities and differences governing the cryopreservation success of koala (Phascolarctos cinereus: goldfuss) and common wombat (Vombatus ursinus: shaw) spermatozoaS D Johnston
School of Animal Studies, University of Queensland, Gatton 4343, Australia
Cryobiology 53:218-28. 2006....
Studies of male reproduction in captive African wild dogs (Lycaon pictus)S D Johnston
School of Animal Studies, The University of Queensland, Gatton 4343, Australia
Anim Reprod Sci 100:338-55. 2007..8+/-5.8%; rate: 2.8+/-0.3; % intact plasma membranes: 33.4+/-5.3% and the % of damaged acrosomes: 4.4+/-1.5%) but following 2 h incubation at 37 degrees C, post-thaw survival declined markedly...
One-sided ejaculation of echidna sperm bundlesS D Johnston
School of Animal Studies, University of Queensland, Gatton, Queensland 4343, Australia
Am Nat 170:E162-4. 2007..Sperm bundling appears to confer increased sperm motility, which may provide the potential for sperm competition between males...
The development of a testosterone stimulation test in the Virginia opossum (Didelphis virginiana) and its use in evaluating deslorelin contraceptionS D Johnston
School of Animal Studies, The University of Queensland, Gatton, Queensland 4343, Australia
Reprod Fertil Dev 20:563-9. 2008....
Assessment of reproductive status in male echidnasS D Johnston
School of Animal Studies, The University of Queensland, Gatton, 4343 Qld, Australia
Anim Reprod Sci 97:114-27. 2007..Testicular ultrasonography and spermatorrhea are useful in assessing reproductive status and in this study were successfully used to determine seasonal reproduction in captive echidnas...
Non-invasive methods of oestrus detection in captive southern hairy-nosed wombats (Lasiorhinus latifrons)L A Hogan
School of Animal Studies, The University of Queensland, Gatton, 4343 Qld, Australia
Anim Reprod Sci 119:293-304. 2010..We therefore recommend the use of 24-h video surveillance as the most reliable method for oestrus detection in captive SHN wombats...
Osmotic stress and cryoinjury of koala sperm: an integrative study of the plasma membrane, chromatin stability and mitochondrial functionS D Johnston
School of Agriculture and Food Science, The University of Queensland, Gatton, Queensland 4343, Australia
Reproduction 143:787-97. 2012....
Monitoring male southern hairy-nosed wombat (Lasiorhinus latifrons) reproductive function and seasonality in a captive populationL A Hogan
School of Animal Studies, The University of Queensland, Gatton, 4343 Qld, Australia
Anim Reprod Sci 118:377-87. 2010....
Collection, seminal characteristics and chilled storage of spermatozoa from three species of free-range flying fox (Pteropus spp.)C E de Jong
The University of Queensland, St. Lucia, 4072 QLD, Australia
Theriogenology 64:1072-89. 2005..Tetracycline, penicillin, ciprofloxacin, and ceftazidime were the most effective antibiotics in preventing growth of all identified bacteria; however, their effects on sperm survival were not investigated...
Oestrus in the Julia Creek dunnart (Sminthopsis douglasi) is associated with wheel running behaviour but not necessarily changes in body weight, food consumption or pouch morphologyK Pollock
School of Animal Studies, The University of Queensland, Gatton 4343, Australia
Anim Reprod Sci 117:135-46. 2010..The majority of the pouch variables examined showed maximum development during the inter-oestrus period but as there was considerable variation between animals, the pouch was not considered a useful index of oestrus...
Testosterone secretion and pharmacological spermatozoal recovery in the cane toad (Bufo marinus)E Iimori
School of Animal Studies, The University of Queensland, Gatton, Australia
Anim Reprod Sci 90:163-73. 2005..The hCG protocols developed in this study will have application in studies on the reproductive biology of rare and endangered male anurans...
Flying-fox (Pteropus spp.) sperm membrane fatty acid composition, its relationship to cold shock injury and implications for cryopreservation successD F Melville
School of Agriculture and Food Science, University of Queensland, Gatton, QLD 4343, Australia
Cryobiology 65:224-9. 2012..5). Membranes containing more PUFAs are more fluid, so the use of cryogenic media which improves membrane fluidity should improve Pteropus spp. spermatozoal viability post-thaw...
Studies on the cryopreservation of common wombat (Vombatus ursinus) spermatozoaC MacCallum
School of Animal Studies, The University of Queensland, Gatton, Australia
Reprod Fertil Dev 17:727-32. 2005..The present study also reports, for the first time, a successful pellet method of freezing wombat spermatozoa on dry ice; volumes of 0.25 and 0.5 mL resulted in higher post-thaw survival compared with 0.1-mL pellets...
Relationship between thirty post-thaw spermatozoal characteristics and the field fertility of 11 high-use Australian dairy AI siresN J Phillips
School of Veterinary Science, The University of Queensland, Brisbane, QLD 4072, Australia
Anim Reprod Sci 81:47-61. 2004....
Expression of FGF2 and TGFalpha and testis morphology during testicular hypertrophy subsequent to hemicastration in the neonatal boarR Wells
School of Animal Studies, The University of Queensland, Gatton, Australia
Mol Reprod Dev 75:961-6. 2008..It was concluded that upregulation of TGFalpha or FGF2 expression is not a pre-requisite for enhanced testicular growth and increased Sertoli cell proliferation that occurs subsequent to hemicastration in the neonatal boar...
Cryopreservation of spermatozoa of black marlin, Makaira indica (Teleostei: Istiophoridae)K M van der Straten
School of Animal Studies, The University of Queensland, Gatton, Australia
Cryo Letters 27:203-9. 2006..001) but spermatozoa frozen in 2.5% DMSO showed higher motility than those frozen in 5.0% DMSO (P = 0.014). Pellet size had no effect on post-thaw motility (P = 0.179)...
Directional mapping of DNA nicking in ejaculated and cauda epididymidal spermatozoa of the short-beaked echidna (Tachyglossus aculeatus: Monotremata)S D Johnston
School of Animal Studies, The University of Queensland, Gatton, QLD 4343, Australia
Reprod Fertil Dev 21:1008-14. 2009....
Reproductive seasonality and the effect of the GnRH agonist deslorelin as a contraceptive in captive male Black Flying-foxes (Pteropus alecto)D F Melville
University of Queensland, School of Agriculture and Food Science, Gatton, Queensland, Australia
Theriogenology 77:652-61. 2012....
Using a GnRH agonist to obtain an index of testosterone secretory capacity in the cockatiel (Nymphicus hollandicus) and sulphur-crested cockatoo (Cacatua galerita)E M Lovas
School of Veterinary Science, The University of Queensland, St Lucia, Queensland, Australia
Aust Vet J 88:52-6. 2010..The major aim was to characterise testosterone secretion after injection of a gonadotropin-releasing hormone agonist (GnRHa), then use the test to investigate seasonal reproduction in the male cockatiel...
Venipuncture in the short-beaked echidnaS D Johnston
School of Animal Studies, The University of Queensland, Gatton, Queensland 4343
Aust Vet J 84:66-7. 2006
Identifying the sex of short-beaked echidnasS D Johnston
School of Animal Studies, The University of Queensland, 4343
Aust Vet J 84:63-5. 2006
Reproductive physiology of the female greater bilby (Macrotis lagotis Thylacomyidae): evidence for a male-induced luteal phaseK Ballantyne
School of Animal Studies, University of Queensland, Gatton, QLD 4343, Australia
Reprod Fertil Dev 21:274-82. 2009..Similar to female reproductive physiology in the Peramelidae, elevated progestogen concentration in the greater bilby was extended into lactation...
Non-invasive monitoring of male and female numbat (Myrmecobius fasciatus: Myrmecobiidae) reproductive activityL A Hogan
Perth Zoo, South Perth, WA, Australia
Anim Reprod Sci 133:237-45. 2012..Collectively, results of the present study confirm that PM and TM can be reliably used to index numbat reproductive activity...
Semen-induced luteal phase and identification of a LH surge in the koala (Phascolarctos cinereus)S D Johnston
School of Animal Studies, The University of Queensland, 4072, Australia
Reproduction 128:629-34. 2004..It was concluded that semen must be involved in the induction of a luteal phase in the koala. The results presented in this study will serve to improve optimal timing and induction of ovulation for artificial insemination in the koala...
Biology of sperm chromatin structure and relationship to male fertility and embryonic survivalM J D'Occhio
School of Animal Studies, Faculty of Natural Resources, Agriculture and Veterinary Science, The University of Queensland, Gatton Campus, Gatton, QLD 4343, Australia
Anim Reprod Sci 101:1-17. 2007..In this regard, it is possible that 'male factor' infertility may emerge as an even more important component in embryonic development...
Birth of a Banteng (Bos javanicus) calf at Western Plains Zoo after fixed time artificial inseminationS D Johnston
School of Animal Studies, The University of Queensland, Gatton
Aust Vet J 80:94-5. 2002
Dynamics of sperm DNA fragmentation in domestic animals II. The stallionC Lopez Fernandez
Departamento de Biologia, Unidad de Genetica, Universidad Autonoma de Madrid UAM, 20849 Madrid, Spain
Theriogenology 68:1240-50. 2007..We suggest that the SCD test can be used as a routine assessment tool for the development and refinement of preservation protocols designed to reduce stallion sperm DNA damage...
Sperm membrane fatty acid composition in the Eastern grey kangaroo (Macropus giganteus), koala (Phascolarctos cinereus), and common wombat (Vombatus ursinus) and its relationship to cold shock injury and cryopreservation successR R Miller
Biology Department, Hillsdale College, Hillsdale, MI, USA
Cryobiology 49:137-48. 2004..Instead, cryogenic tolerance appears related to DHA levels...
Persistent penile frenulum in a nilgai (Boselaphus tragocamelus)B Bryant
Western Plains Zoo, Dubbo, New South Wales 2830
Aust Vet J 82:419-20. 2004
Development of the pulmonary surfactant system in non-mammalian amniotesS D Johnston
Department of Physiology, University of Adelaide, SA 5005, Adelaide, Australia
Comp Biochem Physiol A Mol Integr Physiol 129:49-63. 2001..Thus, despite temporal differences and vastly different lung morphologies, birth strategies and phylogenies, the overall development and maturation of the PS system is highly conserved amongst the amniotes...
Effects of finasteride on size of the prostate gland and semen quality in dogs with benign prostatic hypertrophyK Sirinarumitr
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman 99164, USA
J Am Vet Med Assoc 218:1275-80. 2001..CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that finasteride can be used to reduce prostatic size in dogs with BPH without adversely affecting semen quality or serum testosterone concentration...
